Migration Rules and Mechanisms of Nano-Biochar in Soil Columns under Various Transport Conditions.

Compared to traditional biochar (BC), nano-biochar (NBC) boasts superior physicochemical properties, promising extensive applications in agriculture, ecological environments, and beyond. Due to its strong adsorption and migration properties, NBC may carry nutrients or pollutants to deeper soil layers or even groundwater, causing serious environmental risks. Nevertheless, the migration rules and mechanisms of NBC in soil are still unclear. Therefore, this study employed soil column migration experiments to systematically explore the migration rules and mechanisms of NBC under various flow rates, initial soil water contents, soil depths, and soil textures. The results showed that regulated by smaller particle size differences and greater surface charges, NBC exhibited a stronger migration ability compared with traditional BC. As the soil texture transitioned from fine to coarse, the migration capability of NBC significantly improved, driven by both pore structure and interaction forces as described by the DLVO theory. The migration ability of NBC was also greatly boosted as the soil transitioned from saturated to unsaturated conditions, primarily because of preferential flow. When the flow rate increased from 70% KS to 100% KS and 130% KS, the migration ability of NBC also increased accordingly, as changes in injection flow rates altered the velocity distribution of pore water. NBC in 25 cm soil columns was more prone to shallow retention compared with 10 cm soil columns, resulting in weaker overall migration ability. In addition, through fitting of the two-site kinetic model and related parameters, the penetration curves of NBC under various variable conditions were effectively characterized. These findings could offer valuable insights for NBC's future efficient, rational, and sustainable utilization, facilitating the evaluation and mitigation of its potential environmental risks.

Recent advancement of nano-biochar for the remediation of heavy metals and emerging contaminants: Mechanism, adsorption kinetic model, plant growth and development.

Even though researches have shown that biochar can improve soil-health and plant-growth even in harsh environments and get rid of harmful heavy metals and new contaminants, it is still not sustainable, affordable, or effective enough. Therefore, scientists are required to develop nanomaterials in order to preserve numerous aquatic and terrestrial species. The carbonaceous chemical known as nano-biochar (N-BC) can be used to get rid of metal contamination and emerging contaminants. However, techniques to reduce hetero-aggregation and agglomeration of nano-biochar are needed that lead to the emergence of emerging nano-biochar (EN-BC) in order to maximise its capacity for adsorption of nano-biochar. To address concerns in regards to the expanding human population and sustain a healthy community, it is imperative to address the problems associated with toxic heavy metals, emerging contaminants, and other abiotic stressors that are threatening agricultural development. Nano-biochar can provide an effective solution for removal of emerging contaminants, toxic heavy metals, and non-degradable substance. This review provides the detailed functional mechanistic and kinetics of nano-biochar, its effectiveness in promoting plant growth, and soil health under abiotic stress. Nonetheless, this review paper has comprehensively illustrated various adsorption study models that will be employed in future research.

Green iron oxide nanoparticles and magnetic nanobiochar: enhancing tomato performance, phytochemicals, and root-knot nematode resistance.

BACKGROUND: Green nanoparticles are considered to be an effective strategy for improving phytochemicals and raising productivity in soil infected by root-knot nematodes. This work aims to understand the characteristics of certain nanomaterials, including non-iron (nFe), green non-iron (GnFe), and green magnetic nanobiochar (GMnB), and the effect of adding them at 3 and 6 mg kg- 1 on phytochemicals and tomato (Solanum lycopersicum) plant growth in soils infected by root-knot nematodes. RESULTS: Spectroscopic characterization of nanomaterials showed that nFe, GnFe, and GMnB contained functional groups (e.g., Fe-O, S-H, C-H, OH, and C = C) and possessed a large surface area. Application of GMB at 6 mg kg- 1 was the most efficient treatment for increasing the phytochemicals of the tomato plant, with a rise of 123.2% in total phenolic, 194.7% in total flavonoids, 89.7% in total carbohydrate, 185.2% in total free amino acids, and 165.1% in total tannin compared to the untreated soil. Tomato plant growth and attributes increased with increasing levels of soil nano-amendment in this investigation. The addition of GnFe3 and GnFe6 increased the reduction of root galls of root-knot nematodes by 22.44% and 17.76% compared with nFe3 and nFe6, respectively. The inclusion of the examined soil nano-amendments increased phytochemicals and reduced the total number of root-knot nematodes on tomato plants at varying rates, which played a significant role in enhancing tomato growth. CONCLUSIONS: In conclusion, treating tomato plants with GnFe or GMnB can be used as a promising green nanomaterial to eliminate root-knot nematodes and increase tomato yield in sandy clay loam soil.

Recovery of biochar particles laden with lead in saturated porous media by DC electric field.

The co-transport behavior of environmental pollutants with biochar particles has aroused great interests from researchers due to the concerns about pollutant diffusion and environmental exposure after biochar is applied to soil. In this work, the recovery and co-transport behavior of biochar micron-/nano-particles (BCMP and BCNP) and lead (Pb2+) in saturated porous media were investigated under different ionic strength conditions (IS = 1, 5 and 10 mM) under a direct current electric field. The results showed that the electric field could significantly enhance the mobility of Pb adsorbed biochar particles, particularly BCNP. The recovery of Pb laden biochar particles was improved by 1.8 folds, reaching 78.8% at maximum under favorable condition at +0.5 V cm-1. According to the CDE (Convection-Dispersion-Equation) model and DLVO (Derjaguin-Landau-Verwey-Overbeek) theory analysis, the electric field facilitated the transport of Pb carried biochar mainly by increasing the negative charges on biochar surface and improving the repulsive force between biochar and porous media. High IS was favorable for biochar transport under the electric field, but inhibited desorbing Pb2+ from biochar (18% by maximum at IS = 10 mM). By switching the electric field power, a two-stage strategy was established to maximize the recovery of both biochar particles and Pb, where BCNP and Pb recovery were higher than electric field free case by 90% and 35%, respectively. The findings of this study can help build a biochar recovery approach to prevent potential risks from biochar application in heavy metal contaminated soil remediation.

Biochar and nano biochar: Enhancing salt resilience in plants and soil while mitigating greenhouse gas emissions: A comprehensive review.

Salinity stress poses a significant challenge to agriculture, impacting soil health, plant growth and contributing to greenhouse gas (GHG) emissions. In response to these intertwined challenges, the use of biochar and its nanoscale counterpart, nano-biochar, has gained increasing attention. This comprehensive review explores the heterogeneous role of biochar and nano-biochar in enhancing salt resilience in plants and soil while concurrently mitigating GHG emissions. The review discusses the effects of these amendments on soil physicochemical properties, improved water and nutrient uptake, reduced oxidative damage, enhanced growth and the alternation of soil microbial communities, enhance soil fertility and resilience. Furthermore, it examines their impact on plant growth, ion homeostasis, osmotic adjustment and plant stress tolerance, promoting plant development under salinity stress conditions. Emphasis is placed on the potential of biochar and nano-biochar to influence soil microbial activities, leading to altered emissions of GHG emissions, particularly nitrous oxide(N2O) and methane(CH4), contributing to climate change mitigation. The comprehensive synthesis of current research findings in this review provides insights into the multifunctional applications of biochar and nano-biochar, highlighting their potential to address salinity stress in agriculture and their role in sustainable soil and environmental management. Moreover, it identifies areas for further investigation, aiming to enhance our understanding of the intricate interplay between biochar, nano-biochar, soil, plants, and greenhouse gas emissions.

Physiological and biochemical effects of biochar nanoparticles on spinach exposed to salinity and drought stresses.

The utilization of nanobiochar in agricultural practices has garnered substantial interest owing to its promising potential. Its nano-size particles possess an enhanced ability to infiltrate plant cells, potentially instigating biochemical and physiological responses that augment stress tolerance. In our study, we aimed to assess the impact and extent of exogenously applied nanobiochar on the growth dynamics and antioxidative responses in Spinacia oleracea L. (spinach) plants subjected to salt stress (50 mM NaCl) and drought stress (maintained at 60% field capacity) compared with respective controls (0 mM NaCl and 100% field capacity). Following a 15-day exposure to stress conditions, nanobiochar solution (at concentrations of 0, 1, 3, and 5% w/v) was sprayed on spinach plants at weekly intervals (at 14, 21, and 28 days after sowing). The foliar application of nanobiochar markedly improved biomass, net assimilation rate, leaf area, and various other growth parameters under drought and salinity stress conditions. Notably, the application of 3% nanobiochar caused the most significant enhancement in growth traits, photosynthetic pigments, and nutrient content, indicating its efficiency in directly supplying nutrients to the foliage. Furthermore, under drought stress conditions, the application of 3% nanobiochar elicited a notable 62% increase in catalase activity, a two-fold rise in peroxidase activity, and a 128% increase in superoxide dismutase activity compared to the control (without nanobiochar). Additionally, nanobiochar application enhanced membrane stability, evidenced by reduced lipid peroxidation and electrolyte leakage. The foliar application of 3% nanobiochar was found as a promising strategy to significantly enhance spinach growth parameters, nutrient assimilation, and antioxidative defense mechanisms, particularly under conditions of drought and salinity stress.

Nano-biochar as a potential amendment for metal(loid) remediation: Implications for soil quality improvement and stress alleviation.

Metal(loid) contamination of agricultural soils has become an alarming issue due to its detrimental impacts on soil health and global agricultural production. Therefore, environmentally sustainable and cost-effective solutions are urgently required for soil remediation. Biochar, particularly nano-biochar, exhibits superior and high-performance capabilities in the remediation of metal(loid)-contaminated soil, owing to its unique structure and large surface area. Current researches on nano-biochar mainly focus on safety design and property improvement, with limited information available regarding the impact of nano-biochar on soil ecosystems and crop defense mechanisms in metal(loid)-contaminated soils. In this review, we systematically summarized recent progress in the application of nano-biochar for remediation of metal(loid)-contaminated soil, with a focus on possible factors influencing metal(loid) uptake and translocation in soil-crop systems. Additionally, we conducted the potential/related mechanisms by which nano-biochar can mitigate the toxic impacts of metal(loid) on crop production and security. Furthermore, the application of nano-biochar in field trials and existing challenges were also outlined. Future studies should integrate agricultural sustainability and ecosystem health targets into biochar design/selection. This review highlighted the potential of nano-biochar as a promising soil amendment for enhancing the remediation of metal(loid)-contaminated agricultural soils, thereby promoting the synthesis and development of highly efficient nano-biochar towards achieving environmental sustainability.

Antifungal efficacy of biogenic waste derived colloidal/nanobiochar against Colletotrichum gloeosporioides species complex.

Anthracnose caused by Colletotrichum spp. usually resulting in significant postharvest losses in the banana production chain. This study investigated the inhibitory effect of corn cob colloidal/nanobiochar (CCN) and Gliricidia sepium wood colloidal/nanobiochar (GCN) on the Colletotrichum gloeosporioides species complex. The CCN and GCN materials were synthesized and thoroughly characterized using various techniques, including UV-Vis and Fluorescence spectroscopy. Then after the fungal growth was examined on Potato Dextrose Agar (PDA) media supplemented with different CCN and GCN concentrations of 0.4 - 20 g/L and CCN and GCN with zeolite at various weight percentages of 10% to 50% w/w. Results from the characterization revealed that CCN exhibited a strong UV absorbance peak value of 0.630 at 203 nm, while GCN had a value of 0.305 at 204 nm. In terms of fluorescence emission, CCN displayed a strong peak intensity of 16,371 at 412 nm, whereas GCN exhibited a strong peak intensity of 32,691 at 411 nm. Both CCN and GCN, at concentrations ranging from 1 to 8 and 0.4 - 20 g/L, respectively, displayed notable reductions in mycelial densities and inhibited fungal growth compared to the control. Zeolite incorporation further enhanced the antifungal effect. To the best of our knowledge, this is the first study to demonstrate the promising potential of colloidal/nanobiochar in effectively controlling anthracnose disease. The synthesized CCN and GCN demonstrate promising antifungal potential against Colletotrichum gloeosporioides species complex, offering the potential for the development of novel and effective antifungal strategies for controlling anthracnose disease in Musa spp.

Waste-derived nanobiochar: A new avenue towards sustainable agriculture, environment, and circular bioeconomy.

The greatest challenge for the agriculture sector in the twenty-first century is to increase agricultural production to feed the burgeoning global population while maintaining soil health and the integrity of the agroecosystem. Currently, the application of biochar is widely implemented as an effective means for boosting sustainable agriculture while having a negligible influence on ecosystems and the environment. In comparison to traditional biochar, nano-biochar (nano-BC) boasts enhanced specific surface area, adsorption capacity, and mobility properties within soil, allowing it to promote soil properties, crop growth, and environmental remediation. Additionally, carbon sequestration and reduction of methane and nitrous oxide emissions from agriculture can be achieved with nano-BC applications, contributing to climate change mitigation. Nonetheless, due to cost-effectiveness, sustainability, and environmental friendliness, waste-derived nano-BC may emerge as the most viable alternative to conventional waste management strategies, contributing to the circular bioeconomy and the broader goal of achieving the Sustainable Development Goals (SDGs). However, it's important to note that research on nano-BC is still in its nascent stages. Potential risks, including toxicity in aquatic and terrestrial environments, necessitate extensive field investigations. This review delineates the potential of waste-derived nano-BC for sustainable agriculture and environmental applications, outlining current advancements, challenges, and possibilities in the realms from a sustainability and circular bioeconomy standpoint.

Comprehensive review on recent production trends and applications of biochar for greener environment.

The suitability of biochar as a supplement for environmental restoration varies significantly based on the type of feedstocks used and the parameters of the pyrolysis process. This study comprehensively examines several aspects of biochar's potential benefits, its capacity to enhance crop yields, improve nutrient availability, support the co-composting, water restoration and enhance overall usage efficiency. The supporting mechanistic evidence for these claims is also evaluated. Additionally, the analysis identifies various gaps in research and proposes potential directions for further exploration to enhance the understanding of biochar application. As a mutually advantageous approach, the integration of biochar into agricultural contexts not only contributes to environmental restoration but also advances ecological sustainability. The in-depth review underscores the diverse suitability of biochar as a supplement for environmental restoration, contingent upon the specific feedstock sources and pyrolysis conditions used. However, concerns have been raised regarding potential impacts on human health within agricultural sectors.

Nano-biochar: recent progress, challenges, and opportunities for sustainable environmental remediation.

Biochar is a carbonaceous by-product of lignocellulosic biomass developed by various thermochemical processes. Biochar can be transformed into "nano-biochar" by size reduction to nano-meters level. Nano-biochar presents remarkable physico-chemical behavior in comparison to macro-biochar including; higher stability, unique nanostructure, higher catalytic ability, larger specific surface area, higher porosity, improved surface functionality, and surface active sites. Nano-biochar efficiently regulates the transport and absorption of vital micro-and macro-nutrients, in addition to toxic contaminants (heavy metals, pesticides, antibiotics). However an extensive understanding of the recent nano-biochar studies is essential for large scale implementations, including development, physico-chemical properties and targeted use. Nano-biochar toxicity on different organisms and its in-direct effect on humans is an important issue of concern and needs to be extensively evaluated for large scale applications. This review provides a detailed insight on nanobiochar research for (1) development methodologies, (2) compositions and properties, (3) characterization methods, (4) potentiality as emerging sorbent, photocatalyst, enzyme carrier for environmental application, and (5) environmental concerns.

Physiological effects of some engineered nanomaterials on radish (Raphanus sativus L.) intercropped with pea (Pisum sativum L.).

Intercropping leguminous plant species with non-legume crops could be an effective strategy to maintain soil fertility. Additionally, the application of nano-Zn and Fe in trace amounts can substantially improve the bioavailable fraction of Zn and Fe. We studied the effect of foliar application of some nanomaterials on the agronomic, physio-biochemical attributes under a radish/pea intercropping system. The radish and pea were sprayed with different nanomaterials (Zn-Fe nanocomposite, nZnO, and nanobiochar) at 0 and 50 mg L-1 concentrations. Results indicated that the growth parameters of radish were higher in intercropping than in monocropping, while pea growth was inhibited in intercropping compared with monocropping. The shoot and root length, fresh weight, and dry matter of radish were increased by 28-50%, 60-70%, and 50-56% by intercropping than monocropping. Foliar spray of nano-materials further increased the growth traits of intercropped radish, such as shoot and root length, fresh weight, and dry matter, by 7-8%, 27-41%, and 50-60%, respectively. Similarly, pigments such as chlorophyll a, b, and carotenoids and the concentration of free amino acids, soluble sugars, flavonoids, and phenolics were differentially affected by intercropping and nanomaterials. The yield of the non-legume crop was increased by intercropping, whereas the legume crop exhibited significant growth inhibition due to competitive interactions. In conclusion, both intercropping and foliar spray of nanomaterials could be used as a combined approach to benefit plant growth and enhance the bioavailable Fe and Zn fractions of both crops.

Mechanism for combined application of biochar and Bacillus cereus to reduce antibiotic resistance genes in copper contaminated soil and lettuce.

The remediation of agricultural soil contaminated by antibiotic resistance genes (ARGs) is of great significance for protecting food safety and human health. Reducing the availability of copper in soil may control coresistance to ARGs. However, the feasibility of applying nano-biochar and Bacillus cereus to mitigate the spread of ARGs in Cu contaminated soil remains unclear. Therefore, this study investigated the use of biochar with different particle sizes (2 % apple branch biochar and 0.5 % nano-biochar) and 3 g m-2B. cereus in a 60-day pot experiment with growing lettuce. The effects of single and combined application on the abundances of ARGs in Cu-contaminated soil (Cu = 200 mg kg-1) were compared, and the related mechanisms were explored. Studies have shown that the addition of biochar alone is detrimental to mitigating ARGs in soil-lettuce systems. The combined application of 3 g m-2B. cereus and 0.5 % nano-biochar effectively inhibited the proliferation of ARGs in Cu-contaminated soil, and 3 g m-2B. cereus effectively inhibited the proliferation of ARGs in lettuce. Partial least squares-path modeling and network analysis showed that bacterial communities and mobile genetic elements were the key factors that affected the abundances of ARGs in rhizosphere soil, and Cu resistance genes and bioavailable copper (acid extractable state Cu (F1) + reducing state Cu (F2)) had less direct impacts. The bacterial community was the key factor that affected the abundances of ARGs in lettuce. Rhodobacter (Proteobacteria), Corynebacterium (Actinobacteria), and Methylobacterium (Proteobacteria) may have been hosts of ARGs in lettuce plants. B. cereus and nano-biochar affected the abundances of ARGs by improving the soil properties and reducing the soil bioavailability of Cu, as well as directly or indirectly changing the bacterial community composition in soil and lettuce, thereby impeding the transport of ARGs to aboveground plant parts.

Effect of different aging treatments on the transport of nano-biochar in saturated porous media.

Widely used for soil amendment, carbon sequestration, and remediation of contaminated soils, biochars (BCs) inevitably produce a large number of nanoparticles with relatively high mobility. Geochemical aging alters chemical structure of these nanoparticles and thus affect their colloidal aggregation and transport behavior. In this study, the transport of ramie derived nano-BCs (after ball-milling) was investigated by different aging treatments (i.e., photo (PBC) and chemical aging (NBC)) as well as the managing BC under different physicochemical factors (i.e., flow rates, ionic strengths (IS), pH, and coexisting cations). Consequences of the column experiments indicated aging promoted the mobility of the nano-BCs. Compared to the nonaging BC, consequences of spectroscopic analysis demonstrated the aging BCs exhibited a number of tiny corrosion pores. Both of these aging treatments contribute to a more negative zeta potential and a higher dispersion stability of the nano-BCs, which is caused by the abundance of O-functional groups. Also the specific surface area and mesoporous volume of both aging BCs increased significantly, with the increase being more pronounced for NBC. The breakthrough curves (BTCs) obtained for the three nano-BCs were modelled by the advection-dispersion equation (ADE), which included first-order deposition and release terms. The ADE revealed high mobility of aging BCs, which meant their retention in saturated porous media was reduced. This work contributes to a comprehensive understanding of the transport of aging nano-BCs in the environment.

Effect of nanobiochar (nBC) on morpho-physio-biochemical responses of black cumin (Nigella sativa L.) in Cr-spiked soil.

Chromium is a highly toxic heavy metal. High concentrations of Cr (III) can affect metabolic processes in plants, resulting in different morphological, physiological, and biochemical defects. Agricultural practices such as sewage irrigation, over-fertilization, and sewage sludge application contribute significantly to Cr contamination. It can reduce the growth of plants by affecting the activity of antioxidant enzymes. The materials in nano form play an important role in nano-remediation and heavy metals absorption due to their high surface area and micropores. This research was conducted to study the potential of foliar application of nanobiochar/nBC (100 mg/L-1 and 150 mg/L-1) for mitigation of Cr (III) stress (200 mg/kg and 300 mg/kg) in black cumin (Nigella sativa) plants. The results showed that increased Cr stress (300 mg/kg) decreased the plant growth parameters, chlorophyll content, total soluble sugars, and proteins. However, increased the level of hydrogen peroxide (H2O2) and malondialdehyde acetate (MDA) as a result of the activity of antioxidant enzymes (Catalase, Superoxide dismutase, peroxidase dismutase, and ascorbic peroxidase) increased in Nigella sativa seedlings. Foliar application of the nBC (100 mg/L-1) increased plant growth parameters, chlorophyll content, and osmoprotectants, while decreasing the levels of oxidative stress markers (H2O2 and MDA). Furthermore, with the application of nBC, the antioxidant enzyme activity considerably improved. Improved antioxidant activity shows that nBC helped to decrease oxidative stress, which in return improved the growth of Nigella sativa seedlings. Overall, present study findings concluded that foliar application of nBC in Nigella sativa seedlings improved growth, chlorophyll, and antioxidant enzymes. The nBC treatment of 100 mg/L-1 showed better results compared to 150 mg/L-1 under chromium stress.

Orange-Peel-Derived Nanobiochar for Targeted Cancer Therapy.

Cancer-targeted drug delivery systems (DDS) based on carbon nanostructures have shown great promise in cancer therapy due to their ability to selectively recognize specific receptors overexpressed in cancer cells. In this paper, we have explored a green route to synthesize nanobiochar (NBC) endowed with graphene structure from the hydrothermal carbonization (HTC) of orange peels and evaluated the suitability of this nanomaterial as a nanoplatform for cancer therapy. In order to compare the cancer-targeting ability of different widely used targeting ligands (TL), we have conjugated NBC with biotin, riboflavin, folic acid and hyaluronic acid and have tested, in vitro, their biocompatibility and uptake ability towards a human alveolar cancer cell line (A549 cells). The nanosystems which showed the best biological performances-namely, the biotin- and riboflavin- conjugated systems-have been loaded with the poorly water-soluble drug DHF (5,5-dimethyl-6a-phenyl-3-(trimethylsilyl)-6,6a-dihydrofuro[3,2-b]furan-2(5H)-one) and tested for their anticancer activity. The in vitro biological tests demonstrated the ability of both systems to internalize the drug in A549 cells. In particular, the biotin-functionalized NBC caused cell death percentages to more than double with respect to the drug alone. The reported results also highlight the positive effect of the presence of oxygen-containing functional groups, present on the NBC surface, to improve the water dispersion stability of the DDS and thus make the approach of using this nanomaterial as nanocarrier for poorly water-soluble drugs effective.

High-Energy Ball Milling for High Productivity of Nanobiochar from Oil Palm Biomass.

The current production method of nanobiochar (NBC), an emerging, environmentally friendly nanocarbon material, is tedious and lengthy. Therefore, in this study we aimed to improve the productivity of NBC via high-energy ball milling by manipulating the grinding media and processing time. The particle size distribution of the resulting NBC measured using dynamic light scattering showed that grinding media with steel balls of different sizes were more effective at producing NBC than small uniform steel balls, which failed to produce NBC even after 90 min of milling. Average NBC particles of around 95 nm were achieved after only 30 min of ball milling, and the size was further reduced to about 30 nm when the milling was prolonged to 150 min. Further prolonging the milling duration led to agglomeration, which increased the size of the biochar nanoparticles. The thermogravimetric analysis (TGA) data showed that the duration of milling and particle size did not cause noticeable differences in the thermal stability of the NBC. Based on the FTIR analysis, the chemical structure of the NBC was not affected by the ball milling. The results showed that 60 min of high-energy ball milling is sufficient to produce NBC particles of 75 nm, with a large surface area and high thermal stability. This could prove beneficial in a myriad of applications, ranging from agriculture to composite fabrication.

Novel Insights into the Impact of Nano-Biochar on Composition and Structural Transformation of Mineral/Nano-Biochar Heteroaggregates in the Presence of Root Exudates.

Multiple lines of existing evidence indicate that natural organic matter (NOM) could protect poorly crystalline Fe(III) (oxyhydr)oxides from Fe(II)-catalyzed mineral transformation. Conversely, we find that nano-sized biochar (nano-BC), a pyrogenic form of NOM, promotes the phase transformation of ferrihydrite (Fh) in nano-BC/Fh heteroaggregates in the presence of aqueous Fe(II) and rice root exudates. The nano-BC/Fh heteroaggregates are composed of a core-shell like structure where the inner-layered nano-BC is more compacted and plays the dominant role in accelerating the phase transformation of Fh relative to that in the outer sphere. The extent of phase transformation is more regulated by the reversible redox reactions between quinone and hydroquinone in nano-BC than the electron transfer via its condensed aromatic structures. Furthermore, the reductive organic acids in root exudates contribute to the mineral transformation of nano-BC/Fh associations by donating electrons to Fe(III) through nano-BC. Our results suggest that heteroaggregates between nano-BC and Fe minerals are subjected to partial dissociation during their co-transport, and the stably attached nano-BC is favorable to the phase transformation of poorly crystalline Fe minerals (e.g., Fh), which might have profound implications on biogeochemical cycles of carbon and Fe in the prevailing redox environments.

Synthesis of tri-metallic surface engineered nanobiochar from cynodon dactylon residues in a single step - Batch and column studies for the removal of copper and lead ions.

Superparamagnetic nanocomposites integrated with multiple metals, and surface engineered nanoparticles play a vital role in the removal of heavy metals. In the present study, amino-functional silica-coated magnetic nanocomposites with biochar synthesised from Cynodon dactylon plant residues are prepared in a single step reaction process. The synthesised nanocomposites are characterized using various analytical techniques such as FTIR to determine their functional entities, SEM, TEM, EDX and VSM to analyse the size (~50 nm), elements and magnetic nature of the nanocomposites. Characterization reveals that the prepared nanobiochar was coated with silica and a specific amine group. The magnetic saturation value of 50 emu/g confirms the prepared sorbent was superparamagnetic. Kinetics, isotherm and thermodynamics parameters are evaluated to study the metal interaction mechanism with the nanocomposites where the system follows pseudo-second-order kinetics and the four-parameter Fritz Schlunder model for both metal ions. The nanocomposites showed the enhanced adsorption capacity of copper (Cu(II)) ions with 220.4 mg/g and 185.4 mg/g for lead (Pb(II)) ions. The nanocomposites also showed the excessive reusing ability of 15 times with the maximum removal efficiency for Cu(II) and Pb(II) metal ions. Column studies are evaluated to demonstrate the vital performance in the removal of Cu(II) ions and the breakthrough point was inferred for the parameters such as concentration (100-300 mg/L), bed height (1-3 cm) and flow rate (2-4 mL/min). The breakthrough point was attained at 1400 min and the removal efficiency of about 64.58% was obtained.

Sustainable production and co-immobilization of cold-active enzymes from Pseudomonas sp. for BTEX biodegradation.

Toluene/o-Xylene Monooxygenase (ToMO) is equipped with a broad spectrum of aromatic substrate specificity (such as BTEX; benzene, toluene, ethylbenzene, and isomers of xylenes). TOMO has can hydroxylate more than a single position of aromatic rings in two consecutive monooxygenation reactions. Catechol 1,2-dioxygenase (C1,2D) is an iron-containing enzyme able to cleave the ring of catechol (the converted product from ToMO) for complete detoxification of BTEX. In this study, cold-active ToMO and C1,2D were produced using newly isolated psychrophilic Pseudomonas S2TR-14 in the minimal salt medium supplemented with crustacean waste and different concentrations of used motor oil (0.2-2% (v/v)). Crude ToMO and C1,2D were immobilized into micro/nano biochar-chitosan matrices and used for BTEX biodegradation. The results showed that the highest enzyme production (12 U/mg for ToMO and 22 U/mg for C1,2D) was achieved at the presence of 0.5% v/v used motor oil compared to the control group without motor oil (0.07 and 0.06 U/mg). High immobilization yield was achieved due to covalent bonding of ToMO (92.26% for micro matrix and 77.20% for nano matrix) and C1,2D (87.57% for micro matrix and 74.79% for nano matrix) with matrices. FTIR spectra confirmed the immobilization of enzymes on the surface of microbiochar and nanobiochar-chitosan matrices as proper support. The immobilization increased the storage stability of the enzymes with more than 50% residual activity after 30 days at 4 ± 1 °C, while the free form of enzymes had less than 10% of its activity. Immobilized enzymes degraded more than 80% of BTEX (~200 mg/L in groundwater and ~10,000 mg/kg in soil) at 10 ± 1 °C in groundwater and soil. Therefore, integrated use of microbiochar and nanobiochar with chitosan for co-immobilization of ToMO and C1,2D can be a potential way to remove petroleum hydrocarbons with higher efficiency from contaminated groundwater and soil.