Optimizing the Production of Bacterial Cellulose Nanofibers and Nanocrystals Through Strategic Fiber Pretreatment.

Bacterial cellulose (BC) has unique properties such as high tensile strength, high crystallinity, and high purity. The fiber length of BC causes different attributes. Therefore, the degradation of BC has been studied extensively. In this study, the fibers of BC were rearranged via a DMAc-LiCl solvent and BC was degraded in the wet state. Two different degradation methods were applied: milling with liquid nitrogen and autoclave treatment. The degraded BCs were characterized by FTIR, TEM, AFM, TGA, and XRD. The solvent helps to align the fibers, making them more crystalline. The degraded BCs had a lower crystalline ratio than untreated BC, due to increased hydrogen bonding during degradation in the wet state. Degradation with an autoclave produced two different degraded BCs: nanofibrils and spherical nanocrystals, with and without solvent pretreatment, respectively. The nanofibril lengths were between 312 and 700 nm depending on the applied method, and the spherical nanocrystal size was 56 nm. The rearrangement via solvent causes an important difference in the degradation of BC. Nanofibrils and nanocrystals can be obtained, depending on the rearrangement of fibers before the degradation process.

Crosslinkable Ligands for High-Density Photo-Patterning of Perovskite Nanocrystals.

Perovskite nanocrystals (PNCs) are promising luminescent materials for electronic color displays due to their high luminescence efficiency, widely-tunable emission wavelengths, and narrow emission linewidth. Their application in emerging display technologies necessitates precise micron-scale patterning while maintaining good optical performance. Although photolithography is a well-established micro-patterning technique in the industry, conventional processes are incompatible with PNCs as the use of polar solvents can damage the ionic PNCs, causing severe luminescence quenching. Here, we report the rational design and synthesis of a new bidentate photo-crosslinkable ligand for the direct photo-patterning of PNCs. Each ligand contains two photosensitive acrylate groups and two carboxylate groups, and is introduced to the PNCs via an entropy-driven ligand exchange process. In a close-packed thin film, the acrylate ligands photo-polymerize and crosslink under ultraviolet light, rendering the PNCs insoluble in developing solvents. A high-density crosslinked PNC film with an optical density of 1.1 is attained at 1.4 µm thickness, surpassing industry requirements on the absorption coefficient. Micron-scale patterning is further demonstrated using direct laser writing, producing well-defined 20 µm features. This study thus offers an effective and versatile approach for micro-patterning PNCs, and may also be broadly applicable to other nanomaterial systems.

Effect of Chrysin and Chrysin Nanocrystals on Chlorpyrifos-Induced Dysfunction of the Hypothalamic-Pituitary-Testicular Axis in Rats.

AIMS AND BACKGROUND: The escalating global concerns regarding reproductive health underscore the urgency of investigating the impact of environmental pollutants on fertility. This study aims to focus on Chlorpyrifos (CPF), a widely-used organophosphate insecticide, and explores its adverse influence on the hypothalamic-pituitary-testicular axis in Wistar male rats. This study explores the potential protective effects of chrysin nanocrystal (CHN), a flavonoid with known antioxidant and anti-inflammatory properties, against CPF-induced impairments in male Wistar rats. METHODS: Chrysin nanocrystals were prepared using a solvent precipitation method. Six sets of male Wistar rats were subjected to 30 days of treatment, comprising a control group, a group treated solely with CPF, groups treated with CHN at doses of 5 mg/kg and 10 mg/kg, and groups co-treated with CPF and CHN. Serum levels of reproductive hormones, enzyme biomarkers of testicular function, oxidative stress, and inflammatory biomarkers were assessed. Additionally, histological examinations were conducted on the hypothalamus, testes, and epididymis. RESULTS: CHN exhibited antioxidant and anti-inflammatory properties, effectively counteracting CPF-induced reductions in Luteinizing Hormone (LH), serum testosterone, Follicle-Stimulating Hormone (FSH), and testicular enzyme biomarkers. Moreover, CHN enhanced antioxidant defenses, as evidenced by decreased malondialdehyde (MDA) and increased glutathione (GSH) levels in the hypothalamus, and testes, epididymis. Inflammatory markers, including nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), were significantly reduced in CHN co-treated groups compared to the CPF-only group. Histopathological analyses confirmed the protective effects of CHN on tissue integrity. CONCLUSION: Chrysin nanocrystal demonstrated promising potential in mitigating CPF-induced reproductive deficits in male rats through its anti-inflammatory and antioxidant properties. This study provides valuable insights into therapeutic interventions against environmental toxin-induced reproductive toxicity, emphasizing the potential of chrysin nanocrystals as a protective agent in the context of CPF exposure.

Bioequivalence of Meloxicam Nanocrystal Injection in Healthy Chinese Volunteers.

This single-center, randomized, open, two-preparation, single-dose, two-period, self-crossover trial aimed to assess the bioequivalence and safety of the test (T) preparation compared to the reference (R) preparation following intravenous injection in healthy subjects under fasting conditions. Twenty-four healthy subjects were enrolled in the study and subjects were randomly divided into two groups at a 1:1 ratio and were administered once per period, with an 8-day washout period. During each period, serum drug concentrations were detected for pharmacokinetic analysis and adverse events were recorded for safety analysis. The 90% confidence intervals for the geometric mean ratios (T:R) of maximum serum concentration, area under the serum concentration-time curve from time zero to the last measurable concentration, and area under the serum concentration-time curve from time zero to infinite time fell within the predefined bioequivalence range of 80%-125%, indicating bioequivalence between the T and R preparation under fasting conditions. Additionally, four subjects (16.7%) experienced five instances of adverse events in the T group, while five subjects (21.7%) experienced five instances of adverse events in the R group. This trial indicated the potential bioequivalence between the T and R products under fasting conditions, based on pharmacokinetic and safety profile.

Clustering characteristic diffraction vectors in 4-D STEM data sets from overlapping structures in nanocrystalline and amorphous materials.

We describe a method for identifying and clustering diffraction vectors in four-dimensional (4-D) scanning transmission electron microscopy data to determine characteristic diffraction patterns from overlapping structures in projection. First, the data is convolved with a 4-D kernel, then diffraction vectors are identified and clustered using both density-based clustering and a metric that emphasizes rotational symmetries. The method works well for both crystalline and amorphous samples and in high- and low-dose experiments. A simulated dataset of overlapping aluminum nanocrystals provides performance metrics as a function of Poisson noise and the number of overlapping structures. Experimental data from an aluminum nanocrystal sample shows similar performance. For an amorphous Pd77.5Cu6Si16.5 thin film, experiments measuring glassy structure show strong evidence of 4- and 6-fold symmetry structures. A significant background arises from the diffraction of overlapping structures. Quantifying this background helps to separate contributions from single, rotationally symmetric structures vs. apparent symmetries arising from overlapping structures in projection.

Click-Chemistry-Enabled Functionalization of Cellulose Nanocrystals with Single-Stranded DNA for Directed Assembly.

Controlling the self-assembly of cellulose nanocrystals (CNCs) requires precise control over their surface chemistry for the directed assembly of advanced nanocomposites with tailored mechanical, thermal, and optical properties. In this work, in contrast to traditional chemistries, we conducted highly selective click-chemistry functionalization of cellulose nanocrystals with complementary DNA strands via a three-step hybridization-guided process. By grafting terminally functionalized oligonucleotides through copper-free click chemistry, we successfully facilitated the assembly of brushlike DNA-modified CNCs into bundled nanostructures with distinct chiral optical dichroism in thin films. The complexation behavior of grafted DNA chains during the evaporation-driven formation of ultrathin films demonstrates the potential for mediating chiral interactions between the DNA-branched nanocrystals and their assembly into chiral bundles. Furthermore, we discuss the future directions and challenges that include new avenues for the development of functional, responsive, and bioderived nanostructures capable of dynamic reconfiguration via selective complexation, further surface modification strategies, mitigating diverse CNC aggregation, and exploring environmental conditions for the CNC-DNA assembly.

Effective removal of textile dye via synergy of adsorption and photocatalysis over ZnS nanoparticles: Synthesis, modeling, and mechanism.

In this work, we prepared sulfur-zinc nanoparticles (ZnS-TGA) functionalized with thioglycolic acid by a hydrothermal method and tested their photodegradation ability by solar irradiation. ZnS-TGA were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), high-resolution transmission electron microscope (HR-TEM), UV-Vis spectrophotometer and photoluminescence spectroscopy. In the characterization of these nanoparticles, thioglycolic acid proved to be a strong capping ligand, with a specific surface area of 36.82 m2/g and an average size of 7.15 nm. To test the photocatalytic degradability of the product, methylene blue (MB) was used as a model pollutant. Various operational variables were investigated, including pH, amount of nanoparticles, dye concentration, contact time and temperature. The equilibrium adsorption tests, and the statistical physical calculations allowed the analysis of the energetic and steric variables of the adsorption of MB dye molecules on the surface of these nanoparticles. The equilibrium data were well fitted with Langmuir-Freundlich (L-F) and the adsorption kinetics with pseudo-first order. The maximum adsorption capacity of the MB dye removal process was 30.92 mg g-1 at pH 7 and 298 K, and this process was spontaneous and exothermic. The dye molecules and the surface of the nanoparticles exhibited physical interactions with adsorption energies of 23.31-25.92 kJ/mol. The photocatalytic activity of these nanoparticles resulted in a dye degradation efficiency of 91.1 % in 180 min. The photocatalytic efficiency remained almost unchanged after five consecutive degradation cycles, resulting in a methylene blue degradation of 85 %. According to these results, these environmentally friendly nanoparticles have the potential to purify industrial and urban liquids contaminated with harmful organic compounds such as dye molecules.

Twist elastic constant of chiral nematic cellulose nanocrystals determined by tactoid reconfiguration in electric field.

Lyotropic chiral nematic cellulose nanocrystals (CNCs) have attracted significant attention and great progress has been made. Investigating their physical parameters, especially the twist elastic constant (K22), is pivotal for advancing our comprehension of fundamental viscoelastic property of chiral nematic phase. In this study, we demonstrate a straightforward method to simultaneously estimate K22 and helical twisting power (Kt) of chiral nematic CNCs. This method involves analyzing rheology properties and electro-response of CNCs, focusing on the rotational dynamics and structural reconfiguration of CNC tactoids under an electric field. By examining the rotation dynamics of CNC tactoids under an electric field, together with the viscosity characterization, the anisotropic dielectric susceptibility (∆χ) of chiral nematic CNC along the helix axis was determined. Subsequently, K22/∆χn was extracted by analyzing CNC tactoid pitch evolution under an electric field, employing the de Gennes model. The K22 for different concentrated CNCs is finally estimated by integrating experimental results and theory. It is shown that the chiral nematic CNCs present concentration-dependent K22, ranging from 0.05 to 0.14 pN, while Kt spans from 0.06 to 0.14 pN/µm. This study offers a comprehensive understanding of the CNC fundamental viscoelastic property and opens up new avenues for K22 measurement in other lyotropic liquid crystals.

Enhancement of therapeutic efficacy of Brinzolamide for Glaucoma by nanocrystallization and tyloxapol addition.

BACKGROUND: Brinzolamide (BRI) suspensions are used for the treatment of glaucoma; however, sufficient drug delivery to the target tissue after eye drop administration is hampered by poor solubility. To address this issue, we focused on nanocrystal technology, which is expected to improve the bioavailability of poor-solubility drugs, and investigated the effect of BRI nanocrystal formulations on corneal permeability and intraocular pressure (IOP)-reducing effect. METHODS: BRI nanocrystal formulations were prepared by the wet-milling method with beads and additives. The particle size was measured by NANOSIGHT LM10, and the morphology was determined using a scanning probe microscope (SPM-9700) and a scanning electron microscope (SEM). Corneal permeability was evaluated in vitro using a Franz diffusion cell with rat corneas and in vivo using rabbits, and the IOP-reducing effect was investigated using a rabbit hypertensive model. RESULTS: The particle size range for prepared BRI nanocrystal formulation was from 50 to 300 nm and the mean particle size was 135 ± 4 nm. The morphology was crystalline, and the nanoparticles were uniformly dispersed. In the corneal permeability study, BRI nanocrystallization exhibited higher corneal permeability than non-milled formulations. This result may be attributed to the increased solubility of BRI by nanocrystallization and the induction of energy-dependent endocytosis by the attachment of BRI nanoparticles to the cell membrane. Furthermore, the addition of tyloxapol to BRI nanocrystal formulation further improved the intraocular penetration of BRI and showed a stronger IOP-reducing effect than the commercial product. CONCLUSIONS: The combination of BRI nanocrystallization and tyloxapol is expected to be highly effective in glaucoma treatment and a useful tool for new ophthalmic drug delivery.

Drug Nanocrystals in Oral Absorption: Factors That Influence Pharmacokinetics.

Despite the safety and convenience of oral administration, poorly water-soluble drugs compromise absorption and bioavailability. These drugs can exhibit low dissolution rates, variability between fed and fasted states, difficulty permeating the mucus layer, and P-glycoprotein efflux. Drug nanocrystals offer a promising strategy to address these challenges. This review focuses on the opportunities to develop orally administered nanocrystals based on pharmacokinetic outcomes. The impacts of the drug particle size, morphology, dissolution rate, crystalline state on oral bioavailability are discussed. The potential of the improved dissolution rate to eliminate food effects during absorption is also addressed. This review also explores whether permeation or dissolution drives nanocrystal absorption. Additionally, it addresses the functional roles of stabilizers. Drug nanocrystals may result in prolonged concentrations in the bloodstream in some cases. Therefore, nanocrystals represent a promising strategy to overcome the challenges of poorly water-soluble drugs, thus encouraging further investigation into unclear mechanisms during oral administration.

Graphene-Metal Nanocrystal Hybrid Materials for Bioapplications.

The development of functional nanomaterials is crucial for advancing personalized and precision medicine. Graphene-metal nanocrystal hybrid materials not only possess the intrinsic advantages of graphene-based materials but also exhibit additional optical, magnetic, and catalytic properties of various metal nanocrystals, showing great synergies in bioapplications, including biosensing, bioimaging, and disease treatments. In this Perspective, we discuss the advantages and design principles of graphene-metal nanocrystal hybrid materials and provide an overview of their applications in biological fields. Finally, we highlight the challenges and future directions for their practical implementation.

3D electron diffraction studies of synthetic rhabdophane (DyPO4·nH2O).

In this study, we report the results of continuous rotation electron diffraction studies of single DyPO4·nH2O (rhabdophane) nanocrystals. The diffraction patterns can be fit to a trigonal lattice (P3121) with lattice parameters a = 7.019 (5) and c = 6.417 (5) Å. However, there is also a set of diffuse background scattering features present that are associated with a disordered superstructure that is double these lattice parameters and fits with an arrangement of water molecules present in the structure pore. Pair distribution function (PDF) maps based on the diffuse background allowed the extent of the water correlation to be estimated, with 2-3 nm correlation along the c axis and ∼5 nm along the a/b axis.

Extraction and Physicochemical Characterization of Hydroxyapatites From Horse Humerus Bones of Different Ages (1, 3, 6, and 8 Years old) Calcined at Low Temperature.

The aim of this work is to investigate the changes in the physicochemical properties of hydroxyapatite (HAp) extracted from horse humerus bones of different ages (1, 3, 6, and 8 years) subjected to low temperature calcination (600°C). Thermal analysis revealed significant mass loss due to water, collagen, organic compounds, carbonates, and age-related magnesium out-diffusion. Higher fat content in older bones contributed to increased mass loss. Phosphorus content remained constant across age groups, while calcium and sodium showed age-related fluctuations. Magnesium levels decreased with age, emphasizing its importance for early bone development. The Ca/P ratio deviated from the stoichiometric values due to additional ions from biogenic sources. Infrared spectroscopy identified functional groups in carbonated HAp, with changes observed before and after calcination. The full width at half maximum (FWHM) of the 961 cm-1 band decreased with age, indicating improved crystalline quality. The molar absorption coefficients provided information on the changes in molecular concentration and emphasized the differences between the age groups. X-ray analysis revealed nanocrystalline HAp in all samples, with crystallite size increasing with age. Rietveld analysis showed that the lattice parameters were affected by the presence of organic material, but the lattice constants remained stable, confirming high crystallinity independent of age. TEM analysis confirmed nanocrystalline structures, with crystallite size increasing with age. SEM images showed the characteristic porosity of calcined HAp, with particle size correlating positively with age. Calcination at 600°C preserved the nanoscale properties and microcrystal formation. Raman spectroscopy confirmed the identity of HAp, with FWHM variations indicating age-related changes in crystalline quality. EHAp1 showed increased FWHM, indicating lower crystalline quality and increased trace element content.

Liquid crystal phase behavior of oxalated cellulose nanocrystal and optical films with controllable structural color induced by centripetal force.

Cellulose nanocrystal (CNC) is a sustainable bio-nanomaterial. The distinctive left-handed polarization properties render cellulose nanocrystal a promising candidate for optical film. Due to eco-friendliness, reliability, mildness and simplicity, the oxalate hydrolysis method stands out among various preparation methods for CNC. This study delved into the liquid crystal phase behavior of oxalated cellulose nanocrystal derived from pulp, and discovered the influences of CNC concentration and pH on suspension stability and phase transition, and evaluated its optical properties. The results demonstrated that oxalated CNC presented two different liquid crystal phases, the nematic phase and the cholesteric phase. The stability mechanism of CNC suspension and the regulatory principle of the liquid crystal phase transition were revealed. A novel CNC film-forming technology, the multilayer spin-coating technique, was developed for cellulose nanocrystal optical films. Driven by centrifugal force, cellulose nanocrystals were induced to self-assembly and formed the optical film with circular dichroism and structural color. This simple and efficient film-forming technology promised rapid processing (1 h) and controllable film structure and optical properties compared to traditional technologies. This work provided a theoretical understanding and practical prospects for integrating oxalated cellulose nanocrystal into sustainable advanced optical film materials.

Nanocrystal hydroxyapatite carrying traditional Chinese medicine for osteogenic differentiation.

Developing biomaterials with high osteogenic properties is crucial for achieving rapid bone repair and regeneration. This study focuses on the application of nanocrystal hydroxyapatite (nHAp) as a drug carrier to load Fu Yuan Huo Xue Decoction (FYHXD), a traditional Chinese medicine derived from Angelica sinensis, aiming to achieve improved efficacy in treating bone diseases such as osteoporosis. Through a facile physical adsorption approach, the FTIR result emerges new characteristic absorption peaks in the range of 1200-950 cm-1, proving the successful absorption of FYHXD onto the nHAp with a loading efficiency of 39.76 %. The modified nHAp exhibits a similar shape to the bone-derived hydroxyapatite nanocrystals, and their diameter increases slightly after modification. The drug release assay implies the rapid release of FYHXD in the first 10 h, followed by a continuously slow release within 70 h. The developed nHAp effectively enhances the adhesion, spreading, and proliferation of MC3T3-E1 cells in vitro, and significantly promotes their osteogenic differentiation, as indicated by increased alkaline phosphatase activity. Overall, the biocomposites hold great promise as active ingredients for integration into bone-associated biomaterials, offering the potential to stimulate spontaneous osteogenesis without requiring exogenous osteogenic factors.

Hierarchical Interfacial Construction by Grafting Cellulose Nanocrystals onto Carbon Fiber for Improving the Mechanical Performance of Epoxy Composites.

Carbon fiber-reinforced composites have been widely used in the aerospace industry because of their superior comprehensive performance, including high strength, low density, fatigue resistance, long service life, etc. The interface between the fiber reinforcement and the matrix is one of the key factors that determines the performance of the composites. The construction of covalent bonding connections between the components has proven to be an effective strategy for improving the interfacial bonding strength but always reduces the toughness. In this work, dual silane coupling agents are applied to covalently connect cellulose nanocrystals (CNCs) onto carbon fibers, constructing hierarchical interfacial connections between the fibers and the epoxy matrix and significantly improving the interfacial bonding strength. As a result, the tensile strength of the epoxy composites increased from 519 MPa to nearly 900 MPa, which provides a potential approach for significantly improving the mechanical performance of composites.

High performances of cellulose nanocrystal based bicomponent supramolecular hydrogel lubricant.

To improve the limitations of water-based lubricants, a novel cellulose nanocrystal based supramolecular hydrogel (CNC/x-DG/y) was prepared by mixing cellulose nanocrystal (CNC) and diglycerol (DG) into deionized water (DW). The hydrogel was characterized to determine its material ratio and gelation mechanism. When DW was fixed at 1 mL, CNC content should be no <2.4 wt% and DG content 0.1-1.3 mL. The gelification was driven by the multiple H-bond network between CNC and DG, which immobilized water molecules. The rheological performances, the anti-rust property and the volatilization behaviour of the hydrogel were further studied. The results showed that the hydrogel had satisfactory viscoelasticity, excellent thermal stability, strong creep recovery, high anti-rust performance and low volatilization rate, which were exactly its advantages for use as lubricant. A typical representative of the hydrogel, namely CNC/2.4-DG/0.1, was selected to evaluate the tribological performances, and the resulting worn surfaces were analyzed. CNC/2.4-DG/0.1 exhibited a lower friction coefficient of 0.059 and a smaller wear volume of 0.81 × 10-3 mm3, compared to DW(1 mL) + CNC(2.4 wt%) and DW(1 mL) + DG(0.1 mL). The outstanding tribological performances of CNC/2.4-DG/0.1 were reasonably attributed to the synergistic mending effect of CNC and DG and the dissipative effect of H-bonds between the two.

Nanocrystals: Versatile Platform for Traditional Chinese Medicine Delivery.

The medicinal value of Chinese medicines has been recognized since ancient times, and they have also been used to treat various diseases. However, in-depth studies on the active ingredients of Chinese medicines have shown that many of them suffer from poor water-solubility, stability, and bioavailability, which has severely limited their further development. The advent of nanomedicine represents a novel direction and paradigm for addressing these challenges. Particularly, within the framework of nanocrystal technology, enhancements in the water solubility, stability, and bioavailability of Chinese medicines are expected to significantly improve the therapeutic efficiency. This advancement also holds promise for unlocking new therapeutic capabilities. Nanocrystals offer significant advantages in oral, intravenous, intranasal and targeted delivery. The drug loading principle is "all in one", with hydrophobic-drug-in and hydrophilic-drug-out and stabilization by amphiphilic agents. Nanocrystal technology in traditional Chinese medicine (TCM) holds extensive application potential. Continuous refinement of preparation techniques, sound safety assessments, and the promotion of large-scale production are anticipated to augment its pivotal role in TCM formulations, thereby creating novel opportunities for clinical drug therapy.

Fundamental Aspects of Stretchable Mechanochromic Materials: Fabrication and Characterization.

Mechanochromic materials provide optical changes in response to mechanical stress and are of interest in a wide range of potential applications such as strain sensing, structural health monitoring, and encryption. Advanced manufacturing such as 3D printing enables the fabrication of complex patterns and geometries. In this work, classes of stretchable mechanochromic materials that provide visual color changes when tension is applied, namely, dyes, polymer dispersed liquid crystals, liquid crystal elastomers, cellulose nanocrystals, photonic nanostructures, hydrogels, and hybrid systems (combinations of other classes) are reviewed. For each class, synthesis and processing, as well as the mechanism of color change are discussed. To enable materials selection across the classes, the mechanochromic sensitivity of the different classes of materials are compared. Photonic systems demonstrate high mechanochromic sensitivity (Δnm/% strain), large dynamic color range, and rapid reversibility. Further, the mechanochromic behavior can be predicted using a simple mechanical model. Photonic systems with a wide range of mechanical properties (elastic modulus) have been achieved. The addition of dyes to photonic systems has broadened the dynamic range, i.e., the strain over which there is an optical change. For applications in which irreversible color change is desired, dye-based systems or liquid crystal elastomer systems can be formulated. While many promising applications have been demonstrated, manufacturing uniform color on a large scale remains a challenge. Standardized characterization methods are needed to translate materials to practical applications. The sustainability of mechanochromic materials is also an important consideration.

Self-powered sensing platform for monitoring uric acid in sweat using cobalt nanocrystal-graphene quantum dot-Ti3C2TX monolithic film electrode with excellent supercapacitor and sensing behavior.

The synthesis of cobalt nanocrystal-graphene quantum dot-Ti3C2TX monolithic film electrode (Co-GQD-Ti3C2TX) is reported via self-assembly of Ti3C2TX nanosheets induced by protonated arginine-functionalized graphene quantum dot and subsequent reduction of cobalt (III). The resulting Co-GQD-Ti3C2TX shows good monolithic architecture, mechanical property, dispersibility and conductivity. The structure achieves excellent supercapacitor and sensing behavior. The self-charging supercapacitor produced by printing viscous Co-GQD-Ti3C2TX hydrogel on the back of flexible solar cell surface provides high specific capacitance (296 F g-1 at 1 A g-1), high-rate capacity (153 F g-1 at 20 A g-1), capacity retention (98.1% over 10,000-cycle) and energy density (29.6 W h kg-1 at 299.9 W kg-1). The electrochemical chip produced by printing Co-GQD-Ti3C2TX hydrogel on paper exhibits sensitive electrochemical response towards uric acid. The increase of uric acid between 0.01 and 800 µM causes a linear increase in differential pulse voltammetry signal with a detection limit of 0.0032 µM. The self-powered sensing platform integrating self-charging supercapacitor, electrochemical chip and micro electrochemical workstation was contentedly applied to monitoring uric acid in sweats and shows one broad application prospect in wearable electronic health monitoring device.