Facing the solid waste of cotton straw and plastic mulch film mixture in China: Centralized or decentralized pyrolysis facility?

The widespread use of plastic mulch film (PMF) has led to significant environmental pollution, with PMF residues dispersed and mixed with straw and soil, posing challenges for recycling. Here, we proposed the mobile pyrolysis facility for the cotton straw and mulch film mixture (CMM) to mitigate the collection, storage, and transportation costs, while the application of co-pyrolysis technology for CMM conversion could improve the added value of products. Additionally, centralized combustion power generation and centralized pyrolysis systems were also established to evaluate and compare their sustainability from economic and environmental perspectives. Results showed that mobile pyrolysis has better economic performance than the centralized scenarios, due to its high internal rate of return (31 %) and significant net present value (29.21 M USD). Meanwhile, the mobile pyrolysis facility achieved a GWP of -1.298 kgCO2-eq/kg, reducing emissions by 70.79 % and 38.82 % compared to the two centralized scenarios. In conclusion, mobile pyrolysis technology provides a promising solution for PMF residue recycling because of its economically competitive approach with a lower carbon footprint.

Exploring the potential of biochar for the remediation of microbial communities and element cycling in microplastic-contaminated soil.

The detrimental effects of microplastics (MPs) on soil microbial and elemental raise significant environmental concerns. The potential of remediation with biochar to mitigate these negative impacts remains an open question. The remediation effects of biochar derived from corn and cotton straw on MPs concerning soil microorganisms and element cycling were investigated. Specifically, biochar induced substantial remediations in microbial community structure following MP exposure, restoring and fortifying the symbiotic network while exerting dominance over microbial community changes. A combined treatment of biochar and MPs exhibited a noteworthy increase in the abundance of NH4+, NO3-, and available phosphorous by 0.46-2.1 times, reversing the declining trend of dissolved organic carbon, showing a remarkable increase by 0.36 times. This combined treatment also led to a reduction in the abundance of the nitrogen fixation gene nifH by 0.46 times, while significantly increasing the expression of nitrification genes (amoA and amoB) and denitrification genes (nirS and nirK) by 22.5 times and 1.7 times, respectively. Additionally, the carbon cycle cbbLG gene showed a 2.3-fold increase, and the phosphorus cycle gene phoD increased by 0.1-fold. The mixed treatment enriched element-cycling microorganisms by 4.8-9.6 times. In summary, the addition of biochar repaired the negative effects of MPs in terms of microbial community dynamics, element content, gene expression, and functional microbiota. These findings underscore the crucial role of biochar in alleviating the adverse effects of MPs on microbial communities and elemental cycling, providing valuable insights into sustainable environmental remediation strategies.

Combined effects of mulch film-derived microplastics and pesticides on soil microbial communities and element cycling.

Pesticides and microplastics (MPs) derived from mulch film in agricultural soil can independently impact soil ecology, yet the consequences of their combined exposure remain unclear. Therefore, the effects of simultaneous exposure to commonly used pesticides (imidacloprid and flumioxazin) and aged mulch film-derived MPs on soil microorganisms and element cycles in cotton fields were investigated. The combined exposure influenced soil microorganisms, alongside processes related to carbon, nitrogen, and phosphorus cycles, exhibiting effects that were either neutralized or enhanced compared to individual exposures. The impact of pesticides in combined exposure was notably more significant and played a dominant role than that of MPs. Specifically, combined exposure intensified changes in soil bacterial community and symbiotic networks. The combined exposure neutralized NH4+, NO3-, DOC, and A-P contents, shifting from 0.33 % and 40.23 % increase in MPs and pesticides individually to a 40.24 % increase. Moreover, combined exposure resulted in the neutralization or amplification of the nitrogen-fixing gene nifH, nitrifying genes (amoA and amoB), and denitrifying genes (nirS and nirK), the carbon cycle gene cbbLG and the phosphorus cycle gene phoD from 0.48 and 2.57-fold increase to a 2.99-fold increase. The combined exposure also led to the neutralization or enhancement of carbon and nitrogen cycle functional microorganisms, shifting from a 1.53-fold inhibition and 10.52-fold increase to a 6.39-fold increase. These findings provide additional insights into the potential risks associated with combined pesticide exposure and MPs, particularly concerning soil microbial communities and elemental cycling processes.

Enhanced biodegradable polyester film degradation in soil by sequential cooperation of yeast-derived esterase and microbial community.

The degradation of biodegradable plastics poses a significant environmental challenge and requires effective solutions. In this study, an esterase derived from a phyllosphere yeast Pseudozyma antarctica (PaE) enhanced the degradation and mineralization of poly(butylene succinate-co-adipate) (PBSA) film in soil. PaE was found to substitute for esterases from initial degraders and activate sequential esterase production from soil microbes. The PBSA film pretreated with PaE (PBSA-E) rapidly diminished and was mineralized in soil until day 55 with high CO2 production. Soil with PBSA-E maintained higher esterase activities with enhancement of microbial abundance, whereas soil with inactivated PaE-treated PBSA film (PBSA-inact E) showed gradual degradation and time-lagged esterase activity increases. The fungal genera Arthrobotrys and Tetracladium, as possible contributors to PBSA-film degradation, increased in abundance in soil with PBSA-inact E but were less abundant in soil with PBSA-E. The dominance of the fungal genus Fusarium and the bacterial genera Arthrobacter and Azotobacter in soil with PBSA-E further supported PBSA degradation. Our study highlights the potential of PaE in addressing concerns associated with biodegradable plastic persistence in agricultural and environmental contexts.

Occurrence and characteristics of microplastics in soils from greenhouse and open-field cultivation using plastic mulch film.

There is a major knowledge gap concerning the extent of microplastic pollution in agronomic regions of China, which represent a plastic use hotspot. In order to clarify the amendment of agronomic region and plastic film mulching mode to microplastics distribution, the characteristics of microplastics distributed in agricultural soils from three typical regions (Beijing (BJ), Shandong (SD), and Xinjiang (XJ)) with two plastic film mulching modes (greenhouse (G) and conventional field-based film mulching (M)) in China were investigated. Microplastics weight and their response to planting regions were also evaluated in this study. The result showed that the average abundance of microplastics in soils from BJ, SD, and XJ was 1.83 × 104 items kg-1, 4.02 × 104 items kg-1, and 3.39 × 104 items kg-1, and the estimated weight of microplastics per kg of dry soils was 3.12 mg kg-1, 5.63 mg kg-1, and 7.99 mg kg-1, respectively. Microplastics in farmland were mainly of small particle size (50 to 250 µm), with their abundance decreasing with increasing particle size. Among the microplastics detected, polyethylene and polypropylene were the two dominant types present, accounting for 50.0% and 19.7%, respectively. The standard total effect of planting regions on microplastic number and weight was 31.8% and 32.3%, and plastic film mulching modes (G vs. M) could explain 34.4% of the total variation of microplastic compositions with a contribution rate of 65.6% in this study. This research provides key data for an assessment of the environmental risk of microplastics and supports the development of guidelines for the sustainable use of agricultural plastic film. Further, it is necessary to quantify and assess the contribution of other different plastic sources to microplastics in soil. Big data technologies or isotope tracer techniques may be promising approaches.

Soil Microclimate and Persistence of Foodborne Pathogens Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella enterica Newport in Soil Affected by Mulch Type.

Mulching is a common agricultural practice that benefits crop production through soil moisture retention, weed suppression, and soil temperature regulation. However, little is known about the effect of mulch on foodborne pathogens present in soil. In this study, the influence of polyethylene plastic, biodegradable corn-based plastic, paper, and straw mulches on Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella enterica Newport populations in soil was investigated. Silt loam soil in troughs was inoculated with a cocktail of the pathogens and covered with mulch or left bare, then incubated for 21 days, during which bacteria were enumerated and environmental parameters monitored. Bacterial counts declined in all treatments over time (p < 0.001) but persisted at 21 days at 0.8-0.95 log CFU/g. Pathogens also declined as a factor of mulch cover (p < 0.01). An exponential decay with asymptote model fit to the data revealed slower rates of decline in soil under mulches for all pathogens (p < 0.05) relative to bare soil. Compared to the average for all treatments, rates of decay in bare soil were 0.60 (p < 0.001), 0.45 (p < 0.05), and 0.63 (p < 0.001) log CFU/g/d for E. coli O157:H7, L. monocytogenes, and Salmonella, respectively. Linear multiple regression revealed that soil hydrological parameters were positively correlated (p < 0.05) with bacterial counts, while day soil temperatures were negatively correlated (p < 0.001), suggesting that higher day temperatures and lower moisture content of bare soil contributed to the faster decline of pathogens compared to mulched soil. A microcosm experiment using field soil from lettuce cultivation suggested no influence of prior mulch treatment on pathogens. In summary, pathogen decline in soil was modified by the soil microclimate created under mulch covers, but the effect appeared was restricted to the time of soil cover. Slower decline rates of pathogens in mulched soil may pose a risk for contamination of fresh market produce crops.

Integrated Effect of Plastic Mulches and Biorational Insecticides in Managing Tomato Chlorotic Spot Virus (TCSV) and Its Vector Thrips in Tomatoes.

In the USA, tomato chlorotic spot virus (TCSV) was first identified in Miami-Dade County of Florida in 2012. This viral disease is transmitted by thrips (Thysanoptera: Thripidae) of different species, imposing a serious threat to the entire tomato production in the state. Both cultural and chemical control techniques could be essential tools to combat this vector-borne disease. In the present two-year-long study, we determined the effect of different types of plastic mulches and biorational insecticides on managing thrips and TCSV. Results from the leaf and flower samples showed a significantly lower adult thrips population in Entrust®SC treated tomatoes than in other treated and untreated tomatoes in 2018. Silver on black and silver on white reflective plastic mulches significantly reduced the adult thrips population in 2018. In both study years, marketable yield was significantly higher in tomatoes treated with Entrust®SC and reflective plastic mulches than in other treatments. The incidence of TCSV was significantly reduced in tomatoes treated with Entrust®SC and reflective plastic mulches than the untreated control in 2018. Marketable yield was negatively correlated with the thrips population, as observed from the Pearson correlation coefficient analysis. This research describes a potentially viable management program for thrips and thrips-transmitted TCSV.

Plastic mulching, and occurrence, incorporation, degradation, and impacts of polyethylene microplastics in agroecosystems.

Polyethylene microplastics have been detected in farmland soil, irrigation water, and soil organisms in agroecosystems, while plastic mulching is suggested as a crucial source of microplastic pollution in the agroecosystem. Plastic mulch can be broken down from plastic mulch debris to microplastics through environmental aging and degradation process in farmlands, and the colonization of polyethylene-degrading microorganisms on polyethylene microplastics can eventually enzymatically depolymerize the polyethylene molecular chains with CO2 release through the tricarboxylic acid cycle. The selective colonization of microplastics by soil microorganisms can cause changes in soil microbial community composition, and it can consequently elicit changes in enzyme activities and nutrient element content in the soil. The biological uptake of polyethylene microplastics and the associated disturbance of energy investment are the main mechanisms impacting soil-dwelling animal development and behavior. As polyethylene microplastics are highly hydrophobic, their presence among soil particles can contribute to soil water repellency and influence soil water availability. Polyethylene microplastics have been shown to cause impacts on crop plant growth, as manifested by the effects of polyethylene microplastics on soil properties and soil biota in the agroecosystems. This review reveals the degradation process, biological impacts, and associated mechanisms of polyethylene microplastics in agroecosystems and could be a critical reference for their risk assessment and management.

The effect of layers of high tunnel covering and soil mulching on tomato fruit quality.

BACKGROUND: High tunnels (HT) and plastic mulch can increase yield, extend growing seasons, protect plants from harsh weather conditions, and produce more marketable and attractive tomatoes. However, plastic covering reduces solar radiation, which may affect the quality of tomatoes. This study investigated the effects of single-layer versus double-layer HT and plastic mulch versus bare soil on the quality of fully ripe tomato fruits. The study was conducted over 2 years (2021 and 2022). The fruit color (L*, a*, and b*), vitamin C, pH, total titratable acid (TTA), total soluble solids (TSS), and lycopene content of the two tomato cultivars (Brandywine and Rebelski) were quantified. RESULTS: The results showed that quality parameters varied with the plastic layers of HT, plastic mulch, and planting year. The double-layer HT only showed a slight but significant influence on the color of tomatoes of both cultivars in the same year (P < 0.05). Brandywine tomatoes grown in the double-layer HT had significantly lower pH and lycopene content than those grown in single-layer HT, regardless of mulching. The effect of plastic layers on TTA, TSS, and lycopene depended on whether the soil was mulched. Tomatoes grown on bare soil had higher TTA and TSS values than those grown on mulched soil in double-layer HT. Tomatoes grown in single-layer HTs had significantly higher vitamin C content than those in the double-layer HT for both cultivars regardless of mulching. CONCLUSION: This study demonstrates that double-layer HT is unnecessary for the improvement of the overall quality of tomatoes. © 2023 Society of Chemical Industry.

Effects of long-term microplastic pollution on soil heavy metals and metal resistance genes: Distribution patterns and synergistic effects.

Heavy metals (HMs) and microplastics (MPs) are two emerging factors threatening global food security. Whether long-term MPs pollution will affect the distribution of HMs and their resistance genes (MRGs) in soil is unknown. Here, metagenomic approach was used to decipher the fate of MRGs in cropland soils with long-term film MPs residues. Similar distribution pattern of MRGs was formed in long-term film MPs contaminated soil. A total of 202 MRG subtypes were detected, with resistance genes for Multimetal, Cu, and As being the most prevalent type of MRGs. MRGs formed a modular distribution of five clusters centered on MRGs including ruvB in long-term film MPs contaminated soil. MRGs also formed tight co-occurrence networks with mobile genetic elements (MGEs: integrons, insertions and plasmids). Redundancy analysis showed that HMs together with microbial communities and MGEs affected the distribution of MRGs in soil. Thirteen genera including Pseudomonas were identified as potential hosts for MRGs and MGEs. The research provides preliminary progress on the synergistic effect of HMs and MPs in affecting soil ecological security. The synergistic effect of MPs and HMs needs to be considered in the remediation of contaminated soils.

Intensive vegetable production under plastic mulch: A field study on soil plastic and pesticide residues and their effects on the soil microbiome.

Intensive agriculture relies on external inputs to reach high productivity and profitability. Plastic mulch, mainly in the form of Low-Density Polyethylene (LDPE), is widely used in agriculture to decrease evaporation, increase soil temperature and prevent weeds. The incomplete removal of LDPE mulch after use causes plastic contamination in agricultural soils. In conventional agriculture, the use of pesticides also leaves residues accumulating in soils. Thus, the objective of this study was to measure plastic and pesticide residues in agricultural soils and their effects on the soil microbiome. For this, we sampled soil (0-10 cm and 10-30 cm) from 18 parcels from 6 vegetable farms in SE Spain. The farms were under either organic or conventional management, where plastic mulch had been used for >25 years. We measured the macro- and micro-light density plastic debris contents, the pesticide residue levels, and a range of physiochemical properties. We also carried out DNA sequencing on the soil fungal and bacterial communities. Plastic debris (>100 µm) was found in all samples with an average number of 2 × 103 particles kg-1 and area of 60 cm2 kg-1. We found 4-10 different pesticide residues in all conventional soils, for an average of 140 µg kg-1. Overall, pesticide content was ∼100 times lower in organic farms. The soil microbiomes were farm-specific and related to different soil physicochemical parameters and contaminants. Regarding contaminants, bacterial communities responded to the total pesticide residues, the fungicide Azoxystrobin and the insecticide Chlorantraniliprole as well as the plastic area. The fungicide Boscalid was the only contaminant to influence the fungal community. The wide spread of plastic and pesticide residues in agricultural soil and their effects on soil microbial communities may impact crop production and other environmental services. More studies are required to evaluate the total costs of intensive agriculture.

Integrated approach using insecticides in combination with reflective plastic mulch for the management of pepper weevil, Anthonomus eugenii (Coleoptera: Curculionidae).

Pepper weevil, Anthonomus eugenii Cano, is a serious pest of peppers (Capsicum annuum) in the United States and worldwide. This study evaluated the combined effect of insecticides (thiamethoxam and oxamyl) and reflective mulches (reflective silver on white and reflective silver on black) on pepper weevil suppression and yield increase. This study was conducted under field conditions in Homestead, FL, during the fall 2020, spring 2021, and 2022 growing seasons. The study consists of 2 mulch treatments as the main plots (reflective "silver on white," "white on black") and a bare ground with "no mulch" treatment in fall 2020 and spring 2021, and in spring 2022 with an additional treatment of reflective "silver on black" mulch. We hypothesized that jalapeño pepper grown on either reflective "silver on black" or "silver on white" plastic mulches would have fewer pepper weevil adults, fewer pepper weevil infested fruits, and higher yield than those grown on "white on black" plastic mulch or bare soil. Overall, the number of pepper weevil adults on plants and infested fruits was lower in the fall season compared to the spring season. In the fall 2020 season, the number of adults was not significantly different regardless of insecticide treatment and mulch type. However, infested fruits were higher on the untreated control plots than the treated plots in Fall 2020. Marketable yield was not significantly different among insecticide treatment on the "white on black" and reflective "silver on white" mulches. However, thiamethoxam treated plots had significantly higher yield than on the rest of the treatment on the bare soil with "no mulch." The number of adults was higher in the untreated control plot than the treated plots in spring 2021. The number of infested fallen fruits was not significantly different. However, marketable yield was significantly different among treatments on the "white on black" and reflective "silver on white" mulches in 2021. The treated plots on the reflective "silver on white" mulch produced significantly higher yield than the untreated control plots in 2022. The result of this study suggests that growing jalapeño peppers on reflective plastic mulch ("silver on white") in integration with thiamethoxam or oxamyl has the potential in reducing pepper weevil adult population and increasing yield of jalapeño pepper.

The Effect of Wool Mulch on Plant Development in the Context of the Physical and Biological Conditions in Soil.

Mulching techniques can comprise a solution that better utilizes precipitation and irrigation water in such a manner that mitigates soil degradation and drought damage; however, there are still gaps in the literature with regard to the effect of the use of mulch materials on the development of plant-soil-microbe interactions. Waste fibers, as alternative biodegradable mulch materials, are becoming increasingly prominent. The effect of wool mulch (WM) on water use efficiency, with regard to pepper seedlings, was investigated in different soil types (sand, clay loam, peat) in a pot experiment. Two semi-field experiments were also set up to investigate the effect of WM-plant interactions on sweet pepper yields, as compared with agro textiles and straw mulches. Soil parameters (moisture, temperature, DHA, ß-glucosidase enzymes, permanganate-oxidizable carbon) were measured during the growing season. The effect of WM on yield and biomass was more significant with the less frequent irrigation and the greater water-holding capacity of soils. Microbiological activity was significantly higher in the presence of plants, and because of the water retention of WM, the metabolic products of roots and the more balanced soil temperature were caused by plants. In the sandy soil, the straw mulch had a significantly better effect on microbiological parameters and yields than the agro textiles and WM. In soils with a higher water capacity, WM is a sustainable practice for improving the biological parameters and water use efficiency of soil. The effect of WM on yields cannot solely be explained by the water retention of the mulch; indeed, the development of biological activity and plant-soil-microbe interactions in the soil are also contributing factors.

Microplastics in agricultural soils in China: Sources, impacts and solutions.

The detection of microplastics (MPs) in agricultural soils has raised alarms on their potential impacts on agricultural production, particularly in China where agriculture has great importance for domestic consumption and export. This review aims to present the abundance, sources and impacts of MPs in the agricultural soils of China. It has the novelty of synthesizing sustainable agronomic practices to reduce MPs pollution of agricultural soils based on the sources identified. According to the extant study, the abundance of MPs in the agricultural soils in China ranged from 4.94 items/kg in the lower reaches of Yangtze River to 40,800 items/kg in Yunnan Province. The MPs were predominantly ≤1 mm and were mainly composed of fragments, films and fibers. Polyethylene and polypropylene MPs were most reported. Plastic mulching films were the most significant source of MPs in agricultural soils, followed by abandoned greenhouses and the use of organic fertilizers containing fugitive MPs or whose sources were often MPs-polluted. MPs were found to alter soil physicochemical properties for instance, water flow, water-stable aggregates, soil aggregation, soil pH, bulk density and nutrient contents. MPs also affect soil biota through changing the richness and diversity of soil microbial community while retarding growth and disrupting physiological functions of soil macrofauna. The effects of MPs on crops vary and range from alteration of biomass, metabolism and nutrient demands to impacted photosynthesis. Sustainable solutions include the use of grass clippings - straw mix as organic mulches, the use of compost as soil amendment in conjunction with grass-straw mix and incorporation of weed-suppressing biomass into compost, the use of jute and biodegradable plastics for greenhouses, proper decommissioning of abandoned greenhouses as well as setting standards for allowable MPs contents in organic fertilizers and irrigation water.

Impact of plastic mulching as a major source of microplastics in agroecosystems.

The contamination of agroecosystems by microplastics (MPs) has raised great concerns recently. Plastic mulching has contributed a lot in the building of MP pollution in farmlands. This technique has been in use for decades worldwide because of its immense advantages, preferably in drier and colder regions. The physical extraction of plastic mulches at the end of the growing season is very laborious and ineffective, and thus small pieces of mulches are left in the field which later convert into MP particles after aging, weathering, or on exposure to solar radiation. MPs not only influence physical, chemical, or biological properties of soils but also reduce crop productivity which could be a threat to our food security. They also interact with and accumulate other environmental contaminants such as microbial pathogens, heavy metals, and persistent organic pollutants on their surfaces which increase their risk of toxicity in the environment. MPs also transfer from one trophic level to the other in the food chain and ultimately may impact human health. Because of the ineffectiveness of the recovery of plastic film fragments from fields, researchers are now mainly focusing on alternative solutions to conventional plastic mulch films such as the use of biodegradable mulches. In this review, we have discussed the issue of plastic mulch films in agroecosystems and tried to link already existing knowledge to the current limitations in research on this topic from cropland soils and future prospects have been identified and proposed.

Field application of biodegradable microplastics has no significant effect on plant and soil health in the short term.

Bioplastics (biodegradable plastics) potentially offer an encouraging alternative to conventional (petroleum-based) plastics. In practice, bioplastics inevitably generate a large number of bio-microplastics (bio-MPs, diameter <5 mm) during the degradation progress. However, the impact of bio-MPs on plant and soil health within agroecosystems remains incomplete. Here, a field study was conducted to investigate the effect of two shapes (fiber and powder) of pure polylactic acid (PLA) bio-MPs on oat (Avena sativa L.) and soybean (Glycinemax (L.) Merr.) growth and soil health. Our results showed that PLA application at a representative soil loading rate of 0.2% (w/w) had no significant effect on soil enzyme activities, soil physicochemical properties (soil water content, pH, etc.), root characteristics, plant biomass, and crop yield. Thus, we conclude that soil quality, plant health, and ecosystem multifunctionality were not affected by PLA over one growing season (5 months) in the presence of either bio-MP shape (fiber and powder) for either crop species (oat and soybean). Overall, PLA based bio-MPs may not pose a significant threat to agroecosystem functions in the short term (days to months) in the field, thus may provide a viable environmentally benign solution to replace traditional non-biodegradable plastics in agroecosystems.

Tracking antibiotic resistance genes in microplastic-contaminated soil.

Agricultural soils and microplastics (MPs) are hotspots for antibiotic resistance genes (ARGs). Plastic mulch is the most important source of MPs in agricultural soil. ARGs, mobile genetic elements (MGEs), and their host profiles in long-term mulch MP-exposed soils remain unclear. In the present study, metagenomics was used to investigate the distribution patterns of ARGs and MGEs in eight Chinese provinces with a long history of plastic mulch use. A total of 204 subtypes of ARGs and thousands of MGEs (14 integrons, 28 insertions, and 2993 plasmids) were identified. A similar diversity of ARGs was found among MPs film-contaminated sites. The types of ARGs with a high abundance were more concentrated, and multidrug resistance genes were the dominant ARGs. Soils from regions with a longer history of plastic film use (such as Xinjiang province) had a higher abundance of ARGs and MGEs. The distribution of ARGs and MGEs exhibited a modular network distribution pattern. A total of 27 ARG subtypes and 29 MGEs showed co-occurrence network relationships. More than 10 common hosts of ARGs and MGEs, such as Pseudomonas, were found, and their abundances were highest in three provinces, including Xinjiang. This study may help elucidate the impact mechanism of long-term MP residues on the occurrence and spread of ARGs in soil.

Weed Management Strategies for Tomato Plasticulture Production in Florida.

Florida is the top producer of fresh market tomatoes in the U.S., with an average production of 0.4 million metric tons. Tomatoes are commercially grown on plastic mulched raised beds in Southwest Florida, the primary production region in the state. Low tomato yield in plasticulture production is often associated with the poor control of nutsedge species. Nutsedge management, therefore, remains a critical production challenge for tomato growers in Florida. Sandy soil in this region promotes herbicide movement after heavy rainfall or irrigation, affecting weed suppression. This will also potentially impact the timely establishment of new tomato transplants and, consequently, the crop vigor if the herbicides get into the root zone. This review aims to present and discuss an overview of available options to safely manage major weeds of tomatoes, including nutsedge species, in plasticulture production. In addition, this review seeks to discuss an approach for utilizing herbicide adjuvants, such as spray deposition agents or oil binding agents, to improve herbicides' efficacy and tomato crop safety by enhancing their retention in plastic mulched raised beds.

[Response Characteristics of Soil Microbial Community Under Long-term Film Mulching].

Film mulching is an important practice to increase the yield and income in agricultural production. Soil samples were collected from four farmland sites with different mulching years to reveal the effect of long-term plastic mulching on characteristics of soil microbial community structure. In order to explore the long-term effect of soil microbial community change and its effect on the microbial ecological environment, high-throughput sequencing technology was used to analyze the changes in soil bacterial and fungal community structure. The results showed that long-term film mulching had no significant effect on soil bacterial diversity but decreased fungal diversity. Long-term film mulching decreased the abundance of Acidobacteriota and Mortierellomycetes and increased the abundance of Actinobacteriota. Long-term film mulching enriched the beneficial microbial communities such as Bacillus, Nocardioidaceae, Aspergillus, and Hypocreales in soil. However, long-term film mulching indued a simple and fragile soil fungal co-occurrence network pattern. The unidentified Sordariales under Ascomycota was the only key species in the fungal co-occurrence network, which resulted in potential risks to the ecological environment of the farmland soil. This study provided a theoretical basis for further understanding the effects of long-term film mulching on the ecological and environmental effects of microorganisms in farmland.

Microplastics from agricultural plastic mulch films: A mini-review of their impacts on the animal reproductive system.

Plastic mulch films (PMFs) are widely used to improve crop quality and quantity. Although they provide a range of benefits, they degrade into widespread microplastics (MPs), which can cause an unavoidable risk of environmental problems. The residue of PMFs is a significant source of MPs in soils, which can then spread into various ecosystems and be easily absorbed by organisms due to their small size, and subsequently transported through food chain. Notably, MPs have been found in the human placenta, stool and blood, raising an urgent reminder of the potential dangers of MPs to human health. This review summarizes recent studies concerning the effects of MPs on the reproductive system in soil invertebrates, aquatic animals and rodents of both sexes and the mechanisms by which MPs affect the animal reproductive system. The studies on females demonstrated that MPs decrease oocyte quantity and quality, and induce ovary fibrosis, pyroptosis and apoptosis of granulosa cells. In addition, disrupted integrity of the blood-testis barrier, damaged spermatogenesis and compromised sperm quality have been shown in most studies on male animals. The studies on the mechanisms of these effects have provided evidence that MPs act on the animal reproductive system through reactive oxygen species-related mechanisms by initiating the Wnt/ß-Catenin and NLRP3/Caspase-1 pathways in females, and the Nrf2/HO-1/NF-κB, p38 MAPK and MAPK/Nrf2 pathways in males. Taken together, these studies reveal the reproductive toxicity of MPs from PMF on animals and serve as a reminder to properly dispose of PMF waste.