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The ammonium exceedance discharge from sewage treatment plants has a great risk to the stable operation of subsequent constructed wetlands (CWs). The effects of high ammonium shocks on submerged macrophytes and epiphytic biofilms on the leaves of submerged macrophytes in CWs were rarely mentioned in previous studies. In this paper, the 16S rRNA sequencing method was used to investigate the variation of the microbial communities in biofilms on the leaves of Vallisneria natans plants while the growth characteristics of V. natans plants were measured at different initial ammonium concentrations. The results demonstrated that the total chlorophyll and soluble sugar synthesis of V. natans plants decreased by 51.45% and 57.16%, respectively, and malondialdehyde content increased threefold after 8 days if the initial NH4+-N concentration was more than 5 mg/L. Algal density, bacterial quantity, dissolved oxygen, and pH increased with high ammonium shocks. The average removal efficiencies of total nitrogen and NH4+-N reached 73.26% and 83.94%, respectively. The heat map and relative abundance analysis represented that the relative abundances of phyla Proteobacteria, Cyanobacteria, and Bacteroidetes increased. The numbers of autotrophic nitrifiers and heterotrophic nitrification aerobic denitrification (HNAD) bacteria expanded in biofilms. In particular, HNAD bacteria of Flavobacterium, Hydrogenophaga, Acidovorax, Acinetobacter, Pseudomonas, Aeromonas, and Azospira had higher abundances than autotrophic nitrifiers because there were organic matters secreted from declining leaves of V. natans plants. The analysis of the nitrogen metabolic pathway showed aerobic denitrification was the main nitrogen removal pathway. Thus, the nitrification and denitrification bacterial communities increased in epiphytic biofilms on submerged macrophytes in constructed wetlands while submerged macrophytes declined under ammonium shock loading.
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Compuestos de Amonio , Cianobacterias , Desnitrificación , Nitrógeno/análisis , Humedales , ARN Ribosómico 16S , Nitrificación , BiopelículasRESUMEN
In this paper, the experimentally observed significant increase in yield stress for strain rates beyond 104 s-1 (viscous regime) is explicitly considered in laser shock processing (LSP) simulations. First, a detailed review of the most common high-strain-rate deformation models is presented, highlighting the expected strain rates in materials subject to LSP for a wide range of treatment conditions. Second, the abrupt yield stress increase presented beyond 104 s-1 is explicitly considered in the material model of a titanium alloy subject to LSP. A combined numerical-analytical approach is used to predict the time evolution of the plastic strain. Finally, extended areas are irradiated covering a squared area of 25 × 25 mm2 for numerical-experimental validation. The in-depth experimental residual stress profiles are obtained by means of the hole drilling method. Near-surface-temperature gradients are explicitly considered in simulations. In summary, the conventionally accepted strain rate range in LSP (106-107 s-1) is challenged in this paper. Results show that the conventional high-strain-rate hardening models widely used in LSP simulations (i.e., Johnson Cook model) clearly overestimate the induced compressive residual stresses. Additionally, pressure decay, whose importance is usually neglected, has been found to play a significant role in the total plastic strain achieved by LSP treatments.
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Wastewater containing excess phenolic compounds is considered a major environmental concern due to its adverse impacts on the ecosystem. In this work, an effort has been given to treat the p-cresol from wastewater using Recirculating Packed Bed Biofilm Reactor (RPBBR). The process parameters, namely inoculum dose, pH, and NaCl (w/v) concentration were optimized to enhance the specific growth and obtained to be 14 ml, 7.0, and 1% NaCl (w/100 ml), respectively. Maximum p-cresol removal efficiency of 99.36±0.2% was achieved at 100 mg L-1 of p-cresol. First-order rate constants were found to be 0.70 day-1 and 0.96 day-1 for batch and continuous mode, respectively. The intermediates were analysed using FT-IR and GC-MS analysis. Pseudomonas fluorescens was used to assess bacterial toxicity and observed that the toxicity was reduced in case of treated wastewater. Finally, the performance of continuous RPBBR was better than the batch mode.
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Reactores Biológicos , Aguas Residuales , Reactores Biológicos/microbiología , Ecosistema , Cloruro de Sodio , Espectroscopía Infrarroja por Transformada de Fourier , Biodegradación Ambiental , BiopelículasRESUMEN
The performance, microbial enzymatic activity and microbial community of a sequencing batch reactor (SBR) were explored under instantaneous Cd(II) shock loading. After a 24-h Cd(II) shock loading of 100 mg/L, the chemical oxygen demand and NH4+-N removal efficiencies decreased significantly from 92.73% and 99.56% on day 22 to 32.73% and 43% on day 24, respectively, and then recovered to the normal values gradually. The specific oxygen utilization rate (SOUR), specific ammonia oxidation rate (SAOR), specific nitrite oxidation rate (SNOR), specific nitrite reduction rate (SNIRR) and specific nitrate reduction rate (SNRR) decreased by 64.81%, 73.28%, 77.77%, 56.84% and 52.46% on day 23 in comparison with the absence of Cd(II) shock loading, respectively, and they gradually returned to the normal levels. The changing trends of their associated microbial enzymatic activities including dehydrogenase, ammonia monooxygenase, nitrite oxidoreductase, nitrite reductase and nitrate reductase were in accordance with SOUR, SAOR, SNOR, SNIRR and SNRR, respectively. Cd(II) shock loading promoted the microbial reactive oxygen species production and lactate dehydrogenase release, indicating that instantaneous shock caused oxidative stress and damaged to cell membranes of the activated sludge. The microbial richness and diversity, and the relative abundance of Nitrosomonas and Thauera obviously decreased under the stress of Cd(II) shock loading. PICRUSt prediction showed that Cd (II) shock loading significantly affected Amino acid biosynthesis, Nucleoside and nucleotide biosynthesis. The present results are conducive to take adequate precautions to reduce the adverse effect on bioreactor performance in wastewater treatment systems.
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Cadmio , Microbiota , Nitritos , Aguas del Alcantarillado , Nitrificación , Oxígeno , Reactores Biológicos , Nitrógeno/análisis , Eliminación de Residuos Líquidos/métodosRESUMEN
The sudden change of ammonia loading in high-strength ammonium wastewater treatment can directly affect the system stability by altering microbial community dynamics. To maintain the system stability, the effects of ammonia shock loading on microbial community dynamics must be studied. Two sets of sequencing batch reactors were operated with 6 shock cycles (maximum volumetric loading rate of 1928 mg N/(L·d)). CN system contained both organic carbon and ammonia and N system contained only ammonia. Comparing with N system, CN system operated more stably and had higher nitrite accumulation rate. Free ammonia (FA) was the select stress for the turnover of CN microbial communities, while the N communities didn t shift much. The increase of Nitrosomonas and the appearance of heterotrophic nitrification-aerobic denitrification bacteria in CN system presented its resistance and redundancy against FA impact, while the increase of functional genes exhibited functional genes redundancy which maintained the system stability.
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Compuestos de Amonio , Nitrificación , Aguas Residuales , Amoníaco , Reactores Biológicos/microbiología , Nitritos , Bacterias Aerobias , Desnitrificación , NitrógenoRESUMEN
Volatile fatty acids (VFAs) accumulation caused by shock loading severely hampers the performance and stability of anaerobic digestion. An upflow anaerobic sludge blanket (UASB) was operated to examine its performance, sludge properties, and microbial community behavior during shock loading and recovery with exogenous N-hexanoyl-L-homoserine lactone (C6-HSL). After shock loading, chemical oxygen demand (COD) removal was significantly reduced from 79.09% to 65.63%. The abundance of Methanosarcinales also significantly decreased, which resulted in acetate accumulation (1,163.55 mg/L). Sludge granules disintegrated along with the decrease in extracellular polymeric substances (EPS). After supplying 1 µg/L C6-HSL, COD removal resumed to 75.10%. Furthermore, C6-HSL enhanced the abundance and metabolic activity of aceticlastic methanogens, decreased acetate concentration to 146.87 mg/L, improved EPS secretion, and caused the re-assembly of disintegrated sludges to form large granules. These results advanced our understanding of microbial community behavior and provided an attractive strategy for restoration of UASB recovered from shock loading.
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Matriz Extracelular de Sustancias Poliméricas , Aguas del Alcantarillado , 4-Butirolactona/análogos & derivados , Acetatos , Anaerobiosis , Reactores Biológicos , Eliminación de Residuos LíquidosRESUMEN
To investigate the quasi-shear wave behavior and the underlying microscopic mechanism of an anisotropic solid under dynamic deformation beyond its Hugoniot elastic limit, LiF single crystals are shock-compressed along the [310] low-symmetry crystallographic orientation via normal plate-impact method. Interfacial velocity profiles are measured with a Doppler pin system. Peak normal stresses in samples vary between 1.91 GPa and 3.23 GPa. Under the lowest stress in this study, the resultant wave profile shows typical elastoplastic two-wave structures. In the second lowest stress experiment, an irregularity of the plastic wave or the inelastic deformation wave appears in the wave profile. At two higher stresses, a third wave is found following the elastoplastic two waves propagating along the normal direction. Our observations of three-wave structures in the [310] LiF are in excellent agreement with the simulation result of literature. This fact confirms that the immobilization of dislocations and rotation of slip planes are responsible for the microscopic mechanism of the three-wave propagations in the [310] LiF under uniaxial shock loading. The mechanism of the elastoplastic two-wave to anomalous three-wave structures evolution of material under different peak normal stresses will also be discussed.
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To understand the microbial social behaviors regulated by acyl-homoserine lactones (AHLs) in the upflow anaerobic sludge blanket (UASB) during the restored process after a shock loading, the correlation analyses of AHLs and components of extracellular polymeric substances (EPS), AHLs genes and microbes, and AHLs and microbes were investigated. The results showed that the performance could be restored by regulating influent organic loading rate stage-by-stage. A variation in microbial community and endogenous AHLs was also found during the restoration process. It was found that C14-HSL had improved the synthesis of protein in EPS and resulted in better aggregation of microbes. C4-HSL, as well as C8-HSL and 3-oxoC14-HSL, could prompt the metabolism of acidogenic fermentation bacteria. While 3-oxoC6-HSL was identified as the key signal molecule in enhancing methanogenesis. The present work advanced the understanding of microbial social behaviors and provided an attractive strategy for the restoration of anaerobic digestion after shock loadings.
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Percepción de Quorum , Aguas del Alcantarillado , Acil-Butirolactonas , Anaerobiosis , Conducta SocialRESUMEN
The perineuronal net (PNN) region of the brain's extracellular matrix (ECM) surrounds the neural networks within the brain tissue. The PNN is a protective net-like structure regulating neuronal activity such as neurotransmission, charge balance, and action potential generation. Shock-induced damage of this essential component may lead to neuronal cell death and neurodegenerations. The shock generated during a vehicle accident, fall, or improvised device explosion may produce sufficient energy to damage the structure of the PNN. The goal is to investigate the mechanics of the PNN in reaction to shock loading and to understand the mechanical properties of different PNN components such as glycan, GAG, and protein. In this study, we evaluated the mechanical strength of PNN molecules and the interfacial strength between the PNN components. Afterward, we assessed the PNN molecules' damage efficiency under various conditions such as shock speed, preexisting bubble, and boundary conditions. The secondary structure altercation of the protein molecules of the PNN was analyzed to evaluate damage intensity under varying shock speeds. At a higher shock speed, damage intensity is more elevated, and hyaluronan (glycan molecule) is most likely to break at the rigid junction. The primary structure of the protein molecules is least likely to fail. Instead, the molecules' secondary bonds will be altered. Our study suggests that the number of hydrogen bonds during the shock wave propagation is reduced, which leads to the change in protein conformations and damage within the PNN structure. As such, we found a direct connection between shock wave intensity and PNN damage.
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Matriz Extracelular/metabolismo , Modelos Neurológicos , Red Nerviosa/metabolismo , Enfermedades Neurodegenerativas/metabolismo , Neuronas/metabolismo , Animales , HumanosRESUMEN
Groundwater was a desired alternative for decentralized water supply. However, the presence of iron, manganese and ammonia significantly limited its extensive adoptions. In this study, an innovative gravity-driven membrane (GDM) process has been developed to address such problems. The results indicated that GDM process can efficiently diminish the concentrations of iron, manganese and ammonia, with average removal efficiencies of 97%, 95% and 70%, respectively, since the bio-cake layer on the membrane surface can serve as a dynamic barrier for the foulants rejection. In GDM filtration, the manganese removal was mainly attributed to the synergistic effects between the chemically auto-catalytic oxidation by manganese oxides (MnOx) and biological activity by manganese-oxidizing bacteria (MnOB). Pre-addition of MnOx particles into GDM system could significantly enhance the manganese removal and shorten its ripening time by approximately 50%. During long-term filtration, the fluxes of GDM remained stabilized (4-5 L m-2 h-1), and MnOx particles pre-additions could improve the stable fluxes by 23%-37%. The flux stabilization of GDM process was mainly determined by the heterogeneous structures of bio-cake layer, and the generated iron and manganese oxides would improve its heterogeneities. Furthermore, MnOx assisted GDM process conferred robust capacities in resisting the shock loading of manganese and ammonia in the feed water, and the highest concentrations of manganese and ammonia were suggested to be less than 2.96 mg/L and 0.9 mg/L, respectively. Therefore, these findings are full of relevance to develop new strategies to treat the iron- and manganese-containing groundwater and promote the extensive application of UF technology for decentralized water supply.
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Agua Subterránea , Purificación del Agua , Filtración , Hierro , Manganeso , UltrafiltraciónRESUMEN
The study aimed at evaluating the nitrogen removal performance of the immobilized denitrification filler, and the influence of shock loading on the high-rate denitrification process. A pilot scale reactor was operated for treatment of aniline production wastewater. The nitrogen removal activity significantly increased in the continuous feed experiments, reaching 5.23 kg N m-3 day-1 on day 31 (30 °C) at Hydraulic Retention Time (HRT) = 10 h. In the impact experiment, the denitrification filler was inhibited by Free Nitrite Acid (FNA) when the shock load flowed 1.5 times into the bioreactor and recovered after the load was restored for 20 h. The high-throughput results demonstrated that the dominant position of the denitrifying bacteria further enhanced in a micro toxic and high-salinity environment, providing a basis for the dominance of the composite denitrifying bacteria and the efficacy of the immobilization technology.
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Desnitrificación , Aguas Residuales , Bacterias , Reactores Biológicos , Nitrógeno , Aguas del AlcantarilladoRESUMEN
In the present work, acute impact of heavy metals on activated sludge was investigated, specifically the release of biopolymers and nitrogenous soluble microbiological products (N-SMP) that significantly impact tertiary effluent quality. Based on the previously reported studies, Hg2+ and Ag+ were selected as representative "non-essential" heavy metals, while Cu2+ was selected as the "essential" heavy metal. Stress tests show that under the present experimental conditions, adding a higher concentration of heavy metals to the activated sludge increases the concentration of biopolymers and SMP in the supernatant; N-SMP increased more significantly than carbonaceous products, implying a greater risk of formation of toxic nitrogenous disinfection by-products or membrane fouling in relevant tertiary treatment processes. The severity of the release of SMP into the supernatant depended on the heavy metal, with an order of Hg2+ > Ag+ > Cu2+ ("non-essential" > "essential") under identical molar concentrations. The mass balance of typical organics (e.g., biopolymers) in SMP and extracellular polymeric substances (EPS) in activated sludge was analyzed, and a negative correlation between the organics in the SMP and tightly bound EPS was observed, implying that a significant fraction of the SMP could be quickly released from the tightly bound EPS under heavy metal shock conditions and could be related to cell response or damage.
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Mercurio , Aguas del Alcantarillado , Biopolímeros , Reactores Biológicos , Nitrógeno , Plata , Aguas ResidualesRESUMEN
To understand the denitrification efficiency and microbial community shift with increasing salinity in salinity adaptation and shock loading process, nitrate (NO3--N), nitrite (NO2--N) and chemical oxygen demand (COD) removal efficiencies were monitored feeding acetate and primary sludge fermentation liquid. During adaptation process, salinity had little effect on NO3--N removal efficiency (>99.0%) with acetate-fed, while for fermentation liquid-fed, it decreased to around 97% at high salinity (>2.5%). Effluent NO2--N was lower than 0.1 mg/L, though obvious fluctuation of NO2--N was observed with fermentation liquid-fed when salinity change. During shock loading process, denitrification process all had slight decrease when the salinity abruptly increased to 5.0%. Traditional denitrifier of Thauera was the dominant genus, and a specialized microbial community of Azoarcus in salinity adaptation and Paracoccus in shock loading for denitrification showed high salinity tolerant. Meanwhile, microbial diversity was enriched with fermentation liquid-fed at high salinity condition.
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Chloroanilines from industrial wastewater can produce adverse effects on biological wastewater treatment systems due to their potential biotoxicity. The performance, nitrogen removal rate, microbial community and enzymatic activity of a sequencing batch reactor (SBR) were evaluated under transient 3-chloroaniline shock loading. After 40 mg/L 3-chloroaniline shock loading of 24 h on day 9, the chemical oxygen demand (COD) removal efficiency decreased from 90.71% on day 8 to 80.57% on day 11, and the NH4+-N removal efficiency reduced from 98.96% on day 8 to 35.51% on day 12. Subsequently, the COD and NH4+-N removal efficiencies gradually recovered to normal value. Compared with the absence of 3-chloroaniline shock loading, the ammonia-oxidizing rate (SAOR), nitrite-oxidizing rate (SNOR), nitrite-reducing rate (SNIRR) and nitrate-reducing rate (SNRR) decreased by 66.19%, 14.49%, 16.20% and 49.38% on day 11, respectively, and then they gradually recovered to normal value. The SAOR, SNOR, SNIRR and SNRR displayed the similar varying trends to the activities of ammonia monooxygenase, nitrite oxidoreductase, nitrite reductase and nitrate reductase, respectively. The appearance of 3-chloroaniline promoted the microbial reactive oxygen species production and lactate dehydrogenase release. The transient 3-chloroaniline shock loading distinctly impacted the microbial richness and diversity. The present research results can provide theoretical basis and technical support for evaluating the effects of transient 3-chloroaniline shock on biological wastewater treatment systems, which is beneficial to take reasonable preventable measures to decrease the adverse effects on the bioreactor performance.
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Microbiota , Aguas del Alcantarillado , Compuestos de Anilina , Reactores Biológicos , Nitrógeno , Eliminación de Residuos LíquidosRESUMEN
Marine anammox bacteria (MAB) were used to treat nitrogen-rich saline wastewater with Fe(III) addition under nitrogen shock loading. Ammonia loading rate (ALR) and nitrite loading rate (NLR) gradually increased from 0.033 and 0.039 to 0.68 and 0.89â¯kg/(m3·d), respectively. With 5â¯mg/L Fe(III) addition, ammonia removal rate (ARR) and nitrite removal rate (NRR) reached maximal values of 0.56 and 0.60â¯kg/(m3·d), respectively. The value of ΔNO2--N/ΔNH4+-N was lower than theoretical ratio due to existing marine Feammox process. The growth rate of MAB was accelerated by Fe(III) and it dominated the reactor (27.70%). Besides, MAB were synergized with Marinicella and Caldithrix to achieve higher total nitrogen removal. Haldane model was proper to analyze and predict the effect resulting from nitrite on the activity of MAB under nitrogen shock loading. Overall, this study provides novel insights into the effect of Fe(III) on MAB treating nitrogen-rich wastewater.
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Bacterias/metabolismo , Compuestos Férricos/química , Nitrógeno/aislamiento & purificación , Aguas Salinas/química , Aguas Residuales/química , Amoníaco/aislamiento & purificación , Amoníaco/metabolismo , Desnitrificación , Nitritos/aislamiento & purificación , Nitritos/metabolismo , Nitrógeno/metabolismoRESUMEN
Martensitic transformation, reverse martensitic transformation, twinning, and detwinning of equiatomic nickelâ»titanium shape-memory alloy (NiTi SMA) under the action of a shock wave are studied using a molecular-dynamics simulation. In the loading process of a shock wave, B2 austenite is transformed into B19' martensite, whereas in the unloading process of the shock wave, B19' martensite is transformed into B2 austenite. With repeated loading and unloading of the shock wave, martensitic transformation occurs along with twinning, but reverse martensitic transformation appears along with detwinning. The mechanisms for the twinning and detwinning of NiTi SMA subjected to a shock wave are revealed in order to lay the theoretical foundation to investigate the shape-memory effect and superelasticity.
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The decentralized bioreactor is a promising process for landfill leachate (LL) treatment, however, it is often confronted with various forms of shock loadings. To explore the robustness of bioreactors to the long-lasting substrate shocks, a long-term study of over 90 days was carried out to investigate the effects of nitrogen (mainly ammonium nitrogen, NH4-N) and organic (in terms of chemical oxygen demand, COD) shock loading on an engineered zeolite-based biofilter with alternative soil-mixed block (SMB) (EZS-biofilter) for treating matured LL. The low-, mid-, and high-strength intensity of matured LL was theoretical defined mainly according to the content of total nitrogen (TN) and COD. The experiment proved that the EZS-biofilter could effectively absorb the substrate shocks in a range of 104, 408, and 1357 mg/L as TN and 178, 590, and 1050 mg/L as COD, corresponding to the low-, medium-, and high-strength LL, respectively. A modified sensitivity index reflected that the nitrogen shock loadings exerted much more predominant influence than COD shock due to the great variation of nitrification/denitrification. The provided information in this study are beneficial for the practical engineered operation of biofilters for treating matured LL.
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Compuestos de Amonio/metabolismo , Nitrógeno/metabolismo , Eliminación de Residuos Líquidos/métodos , Contaminantes Químicos del Agua/metabolismo , Análisis de la Demanda Biológica de Oxígeno , Reactores Biológicos , Filtración , NitrificaciónRESUMEN
Wastewater treatment ecotechnologies such as constructed wetlands and denitrifying bioreactors are commonly perceived as robust and resilient to shock loading, but this has proved difficult to quantify, particularly when comparing different systems. This study proposes a method of quantifying and comparing performance resilience in response to a standard disturbance. In a side-by-side study we compare the treatment performance of four different configurations of wetlands and denitrifying bioreactors subjected to hydraulic shock loads of five times the standard inflow rate of primary treated sewage for five days. The systems consist of: horizontal-flow gravel-bed wetlands (HG); single pass vertical-flow sand or gravel media wetlands followed by carbonaceous denitrifying bioreactors (VS + C and VG + C respectively); and a recirculating anoxic attached-growth bioreactor and vertical sand media wetland followed by carbonaceous denitrifying bioreactors (R(A + VS)+C). Resilience was quantified for Total Suspended Solids (TSS), Five-day Biochemical Oxygen Demand (BOD5) and Total Nitrogen (TN) by time integration of Relative Disturbance in Performance relative to pre-shock loading performance (days equivalent Performance Reduction), and by the Recovery Time after shock loading ceased. The quantification method allowed an unbiased comparison of the four different systems. It highlighted important differences in the resilience for different removal mechanisms associated with the configuration of the wetlands/bioreactor systems. Relative Disturbances in Performance were expressed in comparison to percent daily removal under standard loading, and, for the different pollutants were equivalent to loss of between 0.08 and 2.51 days of removal capacity. Average Recovery Times ranged from zero to three days, with all systems exhibiting substantial recovery even during the five-day shock loading period. This study demonstrated that both the horizontal gravel wetland and the vertical flow wetland systems combined with carbonaceous bioreactors tested are generally resilient to shock loading of five times hydraulic and organic loading for periods of up to five days. Standard quantification of performance resilience to shock loadings or other perturbations has potential application across a wide range of technologies and research fields.
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Reactores Biológicos , Eliminación de Residuos Líquidos/métodos , Humedales , Análisis de la Demanda Biológica de Oxígeno , Desnitrificación , Nitrógeno/análisis , Aguas Residuales/análisisRESUMEN
Heavy metal contamination of source water frequently occurred in developing countries as a result of accidents. To address the problems, most of the previous studies have focused on engineering countermeasures. In this study, we investigated the effects of heavy metals, particularly copper, on the development of antibiotic resistance by establishing a copper shock loading test. Results revealed that co-selection occurred rapidly within 6 h. Copper, at the levels of 10 and 100 mg/L, significantly increased bacterial resistance to the antibiotics tested, including rifampin, erythromycin, kanamycin, and a few others. A total of 117 antimicrobial-resistance genes were detected from 12 types of genes, and the relative abundance of most genes (particularly mobile genetic elements intâ and transposons) was markedly enriched by at least one fold. Furthermore, the copper shock loading altered the bacterial community. Numerous heavy metal and antibiotic resistant strains were screened out and enriched. These strains are expected to enhance the overall level of resistance. More noticeably, the majority of the co-selected antibiotic resistance could sustain for at least 20 h in the absence of copper and antimicrobial drugs. Resistance to vancomycin, erythromycin and lincomycin even could remain for 7 days. The prominent selection pressure by the copper shock loading implies that a real accident most likely poses similar impacts on the water environment. An accidental release of heavy metals would not only cause harm to the ecological environment, but also contribute to the development of bacterial antibiotic resistance. Broader concerns should be raised about the biological risks caused by sudden releases of pollutants by accidents.