Annotated checklist of chigger mites (Trombidiformes: Leeuwenhoekiidae, Trombiculidae and Walchiidae) in Sri Lanka

Chigger mites (Leeuwenhoekiidae, Trombiculidae, and Walchiidae) are parasites of any terrestrial vertebrate. The present study updates the list of chigger mite (Trombiculidae, Leeuwenhoekiidae, and Walchiidae) species recorded from Sri Lanka along with taxonomic classification based on previous investigations since 1946 to date and our observations in western and southern parts of the country. This checklist listed 15 species in 9 genera within the three chigger families. For now, murid species were the host most sampledparasitized by chiggers in Sri Lanka. However, further studies are required to increase the diversity of chigger mites in Sri Lanka and probably the description of new species, which will be possible with morphological and molecular characterization.

Estimation of sex from the osteometric measurements of the femur in a contemporary Sri Lankan population / Estimación del sexo a partir de las medidas osteométricas del fémur en una población contemporánea de Sri Lanka

SUMMARY: Sex estimation from human skeletal remains is of vital importance in the buildup of a biological profile of an individual in medico-legal and bioarchaeological studies. The present study is focused on the estimation of sex from osteometric measurements of the complete femur and its fragmentary parts, and the development of a web based application related to this. Fifteen osteometric measurements were taken from 78 dry cadaveric femurs from the Faculty of Medicine, University of Kelaniya. Using R software, linear discriminant analysis and logistic regression methods were applied to build classification models with the help of the application of a stepwise procedure, to identify the best combination of measurements to estimate the sex of the femur. A cross-validation method was applied to estimate the predictive accuracy of each model. Since the linear discriminant analysis model gave more predictive accuracy than the regression model, we suggest using linear discriminant analysis to estimate the sex using osteometric measurements of the femur. From the whole femur measurements, a formula to determine sex was developed with highest total accuracy of 83 % using four parameters; epicondylar breadth, anteroposterior mid-shaft diameter, bi-trochanter length, and maximum shaft diameter. Similarly, measurements of transverse head diameter and bi-trochanter length with a total accuracy of 76 % for the proximal part of the femur, measurements of anteroposterior mid-shaft diameter with a total accuracy of 77 % for the mid-shaft, and measurements of epicondylar breadth and maximum length of the lateral condyle with a total accuracy of 70 % for the distal part of the femur were identified as significant discriminants to determine sex, and formulae were written accordingly. Average accuracy ranged from 83 % to 70 %, with male accuracy slightly higher than that of females. A web application to estimate the sex of femur using these formulae was developed and this will be of great importance for forensic medicine and bio-archaeological research in Sri Lanka.

RESUMEN: La estimación del sexo a partir de restos óseos humanos en los estudios médico-legales y bioarqueológicos es de vital importancia en la construcción de un perfil biológico de un individuo. El objetivo de este estudio fue la estimación del sexo a partir de medidas osteométricas del fémur completo y sus partes fragmentarias, y el desarrollo de una aplicación web relacionada con esto. Se tomaron quince medidas osteométricas de 78 fémures cadavéricos secos de la Facultad de Medicina de la Universidad de Kelaniya. Utilizando el software R, se aplicaron métodos de análisis discriminante lineal y regresión logística para construir modelos de clasificación con la aplicación de un procedimiento por pasos, para identificar la mejor combinación de medidas y estimar el sexo a partir del fémur. Se aplicó un método de validación cruzada para estimar la precisión predictiva de cada modelo. Dado que el modelo de análisis discriminante lineal proporcionó una mayor precisión predictiva que el modelo de regresión, sugerimos su utilización para estimar el sexo mediante mediciones osteométricas del fémur. A partir de las mediciones del fémur completo, se desarrolló una fórmula para determinar el sexo con la mayor precisión total del 83 % utilizando cuatro parámetros; anchura del epicóndilo, diámetro anteroposterior del tercio medio de la diáfisis, longitud bitrocánter y diámetro máximo de la diáfisis. De manera similar, utilizamos las mediciones del diámetro transversal de la cabeza del fémur y la longitud del bitrocánter con una precisión del 76 % para la parte proximal del hueso, las mediciones del diámetro anteroposterior del tercio medio de la diáfisis se obtuvo con una precisión del 77 %. El ancho del epicóndilo y la longitud máxima del cóndilo lateral con una precisión del 70 % para la parte distal del fémur se identificaron como discriminantes significativos para determinar el sexo y se escribieron fórmulas. La precisión promedio osciló entre el 83 % y el 70 %, siendo la precisión en los hombres ligeramente superior al de las mujeres. Se desarrolló una aplicación web para estimar el sexo del fémur utilizando estas fórmulas y creemos será importante para la medicina forense y la investigación bioarqueológica en Sri Lanka.

Human skeletal remains analysis rom pallemalala shell midden in Southern Sri Lanka / Análisis de restos óseos humanos del vertedero de conchas de pallemalala en el sur de Sri Lanka

SUMMARY: Sri Lanka, an island off the tip of the Southeast of the Indian subcontinent processes the earliest skeletal evidence of anatomically modern Homo sapiens (37,000 B.P.) and the best human skeletal record sequence in the South Asian region. Adding another to the list, the skeletal remains, which belong to Mesolithic culture were found at Pallemalala shell midden in Southern Sri Lanka during scientific archaeological exploration by Postgraduate Institute of Archaeology, University of Kelaniya. Sri Lanka. The aim of the study was to determine the minimum number of human individuals, age, sex, and pathological conditions related to the ancient Pallemalala commiunity. For the primary analysis, 426 bone fragments were available. Out of those, 233 bones were identified as human bones which represent 7 minimum number of individuals. The rest of the collection comprises some animal bones and shell species. The community was predominated by the female population. The identified age categories were around 20 years, between 35-45 years, and over 45 years. The encountered pathological lesions were bone thickening, alveolar resorption, dental abscesses, dental caries, antemorterm tooth loss, calculus deposits and brown colour stains on teeth. Regarding the dietary pattern, it was evident that their diet may have consisted of coarse foodstuffs with an extremely basic dietary chemistry.

RESUMEN: Sri Lanka es una isla en la punta del sureste del subcontinente indio que procesa la evidencia esquelética más antigua del Homo sapiens anatómicamente moderno (37.000 AP) y la mejor secuencia de registros esqueléticos humanos en la región del sur de Asia. Agregando otro elemento a la lista, los restos óseos, que pertenecen a la cultura mesolítica, se encontraron en el vertedero de conchas de Pallemalala en el sur de Sri Lanka durante la exploración arqueológica científica realizada por el Instituto de Postgrado de Arqueología de la Universidad de Kelaniya, Sri Lanka. El objetivo del estudio fue determinar el número mínimo de individuos humanos, edad, sexo y condiciones patológicas relacionadas con la antigua comunidad Pallemalala. Para el análisis se dispuso de 426 fragmentos óseos. De esos, 233 huesos fueron identificados como huesos humanos que representan un número mínimo de 7 individuos. El resto de la colección se componía de algunos huesos de animales y especies de conchas. La comunidad estaba dominada por la población femenina. Las categorías de edad identificadas fueron alrededor de 20 años, entre 35-45 años y mayores de 45 años. Las lesiones patológicas encontradas fueron engrosamiento óseo, reabsorción alveolar, abscesos dentales, caries dental, pérdida de dientes antemortem, depósitos de cálculo y manchas de color marrón en los dientes. En cuanto al patrón dietético, era evidente que su dieta pudo haber consistido en alimentos toscos con una química dietética extremadamente básica.

Purpura fulminans following hump-nosed pit viper (Hypnale hypnale) envenoming: A rare complication of snakebites.

Hump-nosed pit vipers of the genus Hypnale are highly venomous and reputed for the commonest venomous snakebites in Sri Lanka. They frequently cause local manifestations and less commonly cause systemic effects such as acute kidney injury and coagulopathy. There is no antivenom currently available in Sri Lanka for their envenoming. However, more and more complications of Hypnale bites are being recently described. Purpura fulminans, one of the rare complications of snakebites that we report following authentic Hypnale hypnale bite. A 58-year-old female was bitten by a hump-nosed viper and developed bilateral toe gangrenes, ultimately ended up with amputations. She got recovered with loss of toes in both feet for 46 days treatment at hospital.

Snakebite envenoming in different national contexts: Costa Rica, Sri Lanka, and Nigeria.

Snakebite envenoming is a neglected tropical disease that predominantly affects impoverished rural communities in sub-Saharan Africa, Asia, and Latin America. The global efforts to reduce the impact of this disease must consider the local national contexts and, therefore, comparative studies on envenomings in different countries are necessary to identify strengths, weaknesses and needs. This work presents a comparative analysis of snakebite envenomings in Costa Rica, Sri Lanka, and Nigeria. The comparison included the following aspects: (a) burden of envenomings, (b) historical background of national efforts to confront envenomings, (c) national health systems, (d) antivenom availability and accessibility including local production, (e) training of physicians and nurses in the diagnosis and management of envenomings, (f) prevention campaigns and community-based work, (g) scientific and technological platforms in these topics, and (h) international cooperation programs. Strengths and weaknesses were identified in the three contexts and several urgent tasks to improve the management of this disease in these countries are highlighted. This comparative analysis could be of benefit for similar studies in other national and regional contexts.

Leptospirosis: Increasing importance in developing countries.

Leptospirosis is a zoonotic disease caused by the pathogenic helical spirochetes, Leptospira. Symptoms include sudden-onset fever, severe headaches, muscle pain, nausea and chills. Leptospirosis is endemic in developing countries such as Malaysia, India, Sri Lanka, and Brazil where thousands of cases are reported annually. The disease risk factors include the high population of reservoirs, environmental factors, recreational factors, and occupational factors. To end the endemicity of leptospirosis, these factors need to be tackled. The management of leptospirosis needs to be refined. Early diagnosis remains a challenge due to a lack of clinical suspicion among physicians, its non-specific symptoms and a limited availability of rapid point-of-care diagnostic tests. The purpose of this review is to provide insight into the status of leptospirosis in developing countries focusing on the risk factors and to propose methods for the improved management of the disease.

Human Flourishing in Cross Cultural Settings. Evidence From the United States, China, Sri Lanka, Cambodia, and Mexico.

This paper investigates human flourishing in five culturally distinct populations. Empirical differences in human flourishing were examined using the recently proposed Flourish Index (FI) and Secure Flourish Index (SFI). Five domains for human flourishing are proposed for FI: (D1) happiness and life satisfaction; (D2) physical and mental health; (D3) meaning and purpose; (D4) character and virtue; and (D5) close social relationships. Specification of SFI was augmented by an additional financial and material stability domain (D6). Psychometric properties of FI and SFI were examined using data from the SHINE Well-Being Survey. Between June 2017 and March 2018, a total of 8,873 respondents participated in the study - in the US (4083 participants), Sri Lanka (1284 participants), Cambodia (587 participants), China (419 participants), and Mexico (2500 participants). US participants were customers of a financial institution, while non-US participants were clothing industry workers in the supply chain of a global brand. Exploratory and confirmatory factor models were used to validate the proposed indices. An exploratory approach informed analysis for item groupings. Confirmatory factor models were used to investigate the hierarchical structure of the indices. Configural, metric, and partial scalar measurement invariance were established, which not only supported the universal character of the indices but also validated use of the indices for culturally distinct populations. Findings from our study enrich our knowledge about human flourishing in five culturally distinct populations. With the exception of happiness and life satisfaction, respondents in the US, despite enjoying the highest financial and material stability, scored the lowest in all other domains of human flourishing. Respondents in China excelled in close social relationship and health domains. In addition to life satisfaction and happiness, character and virtue were relatively high in Cambodia. Respondents in Mexico, despite having the lowest scores in financial and material stability, had the greatest meaning and purpose to their lives. Respondents in Sri Lanka were the least happy and satisfied with life.

Cleaning old cabinets: revealing the taxonomy of Sri Lankan wolf spiders (Araneae, Udubidae and Zoropsidae).

The fauna of Udubidae and Zoropsidae of Sri Lanka is reviewed, the families are diagnosed and a distribution map is provided. The udubid genus Campostichomma Karsch, 1892 is diagnosed and redescribed, C. manicatum Karsch, 1892 is redescribed, and we propose three new species, based on females: C. harasbedda sp. nov., C. mudduk sp. nov. and C. alawala sp. nov. The zoropsid genus Devendra Lehtinen, 1967 is diagnosed and redescribed, D. seriatus (Simon, 1898), D. pumilus (Simon, 1898) and D. pardalis (Simon, 1898), are redescribed, and two new species are proposed: D. saama sp. nov. and D. amaiti sp. nov.

Occipito-frontal circumference as a personal stature predictor in a group of Sri Lankan adults / Circunferencia occipito-frontal como predicción de la estatura en un grupo de adultos de Sri Lanka

SUMMARY: Accurate estimation of the personal stature is important in calculating body mass index, assessing nutritional status and the risk of cardiovascular disease, prediction of energy needs and in adaptation of cytostatic drug doses. However, various situations such as, patients in emergency or critical care settings or immobilized patients who are bed ridden or in wheelchairs make it difficult or even impossible to obtain the personal stature using standardized methods. Thus, the need for an alternate method of personal stature prediction arises. Despite its potential practical utility, little is known concerning the relationship between occipito-frontal circumference (OFC) of the head and personal stature among the adult Sri Lankans. Therefore, the goal of this study was to propose population and sex specific regression formula for personal stature estimation using the OFC. Personal stature and OFC was recorded in a total of 156 cadavers belonging to 55 - 89 years of age: (male: n=74; female: n=82). Subjects with any craniofacial deformities or history of neurological disorders were excluded from the study. The mean stature (cm) (male:162.08 ± 9.02 ; female:157.04 ± 9.59) and OFC (cm) (male: 54.09 ± 3.28 ; female: 52.88 ± 2.83) of the study subjects were found to be significantly different (P< 0.01) between the sexes. Correlation coefficient between the OFC and personal stature was statistically significant and positive in both males (0.62) and females (0.86) indicating a strong relationship between the two parameters. Linear regression formulae for the prediction of personal stature using the OFC were derived as follows: male: 70.52 + 1.69 (OFC); female: 3.48 + 2.92 (OFC). Stature prediction equations that require a measuring tape as the only tool to record the OFC provides a practical alternative for the height estimation of patients who are unable to stand due to various reasons. It is a simple, cost effective, non invasive and objective method for personal stature prediction. Accurate prediction of a patient's stature will in turn, reduce potential errors that may occur when stature is estimated only by visual observation in emergency or clinical situations. Complementary studies are necessary to evaluate the applicability of these equations in other age groups too.

RESUMEN: La estimación precisa de la estatura es importante para calcular el índice de masa corporal, evaluar el estado nutricional, como también el riesgo de enfermedad cardiovascular, las necesidades energéticas y la adaptación de las dosis de los fármacos citostáticos. Sin embargo, varias situaciones, por ejemplo en situaciones de emergencias médicas o cuidados intensivos, los pacientes inmovilizados en cama o en silla de ruedas, se hace difícil o incluso imposible obtener la estatura usando métodos estandarizados. Por lo tanto, surge la necesidad de un método alternativo de predicción personal de la estatura. A pesar de su potencial utilidad práctica, se sabe poco sobre la relación entre la circunferencia occipito-frontal (COF) de la cabeza y la estatura entre los adultos de Sri Lanka. El objetivo de este estudio fue proponer una fórmula específica para la estimación de la estatura de acuerdo a población y sexo utilizando la COF. La estatura individual y la COF se registraron en un total de 156 cadáveres pertenecientes a individuos de 55-89 años de edad (hombre: n = 74, mujer: n = 82). Los sujetos con deformidades craneofaciales o antecedentes de trastornos neurológicos fueron excluidos del estudio. Se encontró que la estatura media (cm) (varón: 162,08 ± 9,02, mujer: 157,04 ± 9,59) y OFC (cm) (hombre: 54,09 ± 3,28, mujer: 52,88 ± 2,83) era significativamente diferente (P < 0,01) entre los sexos. El coeficiente de correlación entre la COF y la estatura fue estadísticamente significativo y positivo tanto en varones (0,62) como en mujeres (0,86), lo que indica una fuerte relación entre los dos parámetros. Las fórmulas de regresión lineal para la predicción de la estatura utilizando la COF se obtuvieron de la siguiente manera: hombres: 70,52 + 1,69 (COF); mujeres: 3,48 + 2,92 (OFC). Las ecuaciones de predicción de la estatura que requieren una cinta métrica como la única herramienta para registrar la COF proporcionan una alternativa práctica para estimar la altura en aquellos pacientes que no pueden ponerse de pie por diversos motivos. Es un método sencillo, rentable, no invasivo y objetivo para la predicción personal de la estatura. La predicción exacta de la estatura del paciente, a su vez, reducirá los errores potenciales que pueden ocurrir cuando la estatura se estima sólo por observación visual en situaciones de emergencias o clínicas. Estudios complementarios son necesarios para evaluar la aplicabilidad de estas ecuaciones en otros grupos de edad.

Unveiling Members of Colletotrichum acutatum Species Complex Causing Colletotrichum Leaf Disease of Hevea brasiliensis in Sri Lanka.

Colletotrichum is an important fungal genus with great diversity, which causes anthracnose of a variety of crop plants including rubber trees. Colletotrichum acutatum and Colletotrichum gloeosporioides have been identified as the major causative agents of Colletotrichum leaf disease of rubber trees in Sri Lanka based on morphology, pathogenicity, and the analysis of internally transcribed spacer sequences of the nuclear ribosomal DNA. This study has been conducted to investigate the members of the C. acutatum species complex causing rubber leaf disease using a morphological and multi gene approach. For the first time in Sri Lanka, Colletotrichum simmondsii, Colletotrichum laticiphilum, Colletotrichum nymphaeae, and Colletotrichum citri have been identified as causative agents of Colletotrichum leaf disease in addition to C. acutatum s. str. Among them, C. simmondsii has been recognized as the major causative agent.

Causal mechanism and component causes in Mesoamerican-Sri Lankan nephropathy: the moderator's view.

Inspired by Conan Doyle's novels, the pro contender of this Polar View undertakes an elegant exercise in territory common to epidemiology and police investigation and concludes that cyclic episodes of dehydration in individuals exposed to heavy work in very hot climates is the cause of Mesoamerican and Sri Lankan nephropathy. The contender in the opposite camp accepts the idea that dehydration may play a key role in this condition but highlights a different explanation, that it is contaminated water used for rehydration that is the eventual cause of the disease.Causal mechanisms result from the combination of many components, i.e. conditions or events that are needed for the occurrence of the disease. If we adopt a global, extended approach to the problem, it is unlikely that cyclic dehydration is a key component in all cases. While credible in most cases in Central America, the cyclic dehydration hypothesis fails to explain most cases in Sri Lanka, where agrochemicals have been implicated as the most likely cause of this disease. The experience with Balkan nephropathy is an enduring lesson that full clarification of the causal mechanisms behind endemic nephropathies can be a decades-long process. Therefore, action to control Mesoamerican and Sri Lankan nephropathy should not be deferred. Deployable interventions include the provision of uncontaminated water sources, prevention of dehydration at work sites and the application of safety procedures for agrochemicals. The joint application of these interventions will most likely benefit the populations plagued by this endemic disease.

Development of a new polyspecific antivenom for snakebite envenoming in Sri Lanka: Analysis of its preclinical efficacy as compared to a currently available antivenom.

A new whole IgG, freeze-dried, polyspecific antivenom was prepared from the plasma of horses immunized with the venoms of the snakes Daboia russelii, Echis carinatus, Hypnale hypnale, and Naja naja from Sri Lanka. The preclinical neutralizing ability of this antivenom against several toxic and enzymatic activities of these four venoms was analyzed, and compared with that of a batch of VINS antivenom (India) being currently used in Sri Lanka. The activities tested were: lethality, hemorrhagic, in vitro coagulant, proteinase and phospholipase A2. Both antivenoms neutralized, to a different extent, these activities of the venom of D. russelii, E. carinatus, and N. naja. In general, the polyspecific Sri Lankan antivenom was more effective than the Indian antivenom in the neutralization of the venoms of D. russelii and E. carinatus, whereas the Indian antivenom showed a higher efficacy against the venom of N. naja. Regarding H. hypnale, the new Sri Lankan antivenom was effective in the neutralization of all activities tested, whereas the Indian antivenom neutralized lethality but not hemorrhagic, coagulant, proteinase and PLA2 activities, in agreement with the fact that this venom is not included in the immunization mixture for this antivenom. Results suggest that the new polyspecific Sri Lankan antivenom has a satisfactory preclinical neutralizing profile and compares favorably with the Indian antivenom. This is ready to be tested in a clinical trial to evaluate its efficacy and safety in human victims of snakebite envenomings by D. russelii, E. carinatus and H. hypnale in Sri Lanka.

Polymerase chain reaction detection of LeishmaniaDNA in skin biopsy samples in Sri Lanka where the causative agent of cutaneous leishmaniasis is Leishmania donovani

Leishmania donovani is the known causative agent of both cutaneous (CL) and visceral leishmaniasis in Sri Lanka. CL is considered to be under-reported partly due to relatively poor sensitivity and specificity of microscopic diagnosis. We compared robustness of three previously described polymerase chain reaction (PCR) based methods to detectLeishmania DNA in 38 punch biopsy samples from patients presented with suspected lesions in 2010. Both, Leishmaniagenus-specific JW11/JW12 KDNA and LITSR/L5.8S internal transcribed spacer (ITS)1 PCR assays detected 92% (35/38) of the samples whereas a KDNA assay specific forL. donovani (LdF/LdR) detected only 71% (27/38) of samples. All positive samples showed a L. donovani banding pattern upon HaeIII ITS1 PCR-restriction fragment length polymorphism analysis. PCR assay specificity was evaluated in samples containing Mycobacterium tuberculosis, Mycobacterium leprae, and human DNA, and there was no cross-amplification in JW11/JW12 and LITSR/L5.8S PCR assays. The LdF/LdR PCR assay did not amplify M. leprae or human DNA although 500 bp and 700 bp bands were observed in M. tuberculosis samples. In conclusion, it was successfully shown in this study that it is possible to diagnose Sri Lankan CL with high accuracy, to genus and species identification, using Leishmania DNA PCR assays.

Molecular characterisation and disease severity of leptospirosis in Sri Lanka

Leptospirosis is a re-emerging zoonotic disease all over the world, important in tropical and subtropical areas. A majority of leptospirosis infected patients present as subclinical or mild disease while 5-10% may develop severe infection requiring hospitalisation and critical care. It is possible that several factors, such as the infecting serovar, level of leptospiraemia, host genetic factors and host immune response, may be important in predisposition towards severe disease. Different Leptospira strains circulate in different geographical regions contributing to variable disease severity. Therefore, it is important to investigate the circulating strains at geographical locations during each outbreak for epidemiological studies and to support the clinical management of the patients. In this study immunochromatography, microscopic agglutination test and polymerase chain reaction were used to diagnose leptospirosis. Further restriction fragment length polymorphism and DNA sequencing methods were used to identify the circulating strains in two selected geographical regions of Sri Lanka. Leptospira interrogans, Leptospira borgpetersenii and Leptospira kirschneri strains were identified to be circulating in western and southern provinces. L. interrogans was the predominant species circulating in western and southern provinces in 2013 and its presence was mainly associated with renal failure.

Human CD8+ T-Cell Responses Against the 4 Dengue Virus Serotypes Are Associated With Distinct Patterns of Protein Targets.

BACKGROUND: All 4 dengue virus (DENV) serotypes are now simultaneously circulating worldwide and responsible for up to 400 million human infections each year. Previous studies of CD8(+) T-cell responses in HLA-transgenic mice and human vaccinees demonstrated that the hierarchy of immunodominance among structural versus nonstructural proteins differs as a function of the infecting serotype. This led to the hypothesis that there are intrinsic differences in the serotype-specific reactivity of CD8(+) T-cell responses. METHODS: We tested this hypothesis by analyzing serotype-specific CD8(+) T-cell reactivity in naturally infected human donors from Sri Lanka and Nicaragua, using ex vivo interferon γ-specific enzyme-linked immunosorbent spot assays. RESULTS: Remarkably similar and clear serotype-specific patterns of immunodominance in both cohorts were identified. Pooling of epitopes that accounted for 90% of the interferon γ response in both cohorts resulted in a global epitope pool. Its reactivity was confirmed in naturally infected donors from Brazil, demonstrating its global applicability. CONCLUSIONS: This study provides new insight into differential serotype-specific immunogenicity of DENV proteins. It further provides a potentially valuable tool for future investigations of CD8(+) T-cell responses in the typically small sample volumes available from patients with acute fever and children without requiring prior knowledge of either infecting DENV serotype or HLA type.

Morphometric evaluation of the greater palatine foramen in adult Sri Lankan skulls / Evaluación morfométrica del foramen palatino mayor en craneos adultos de Sri Lanka

Evidence supports a clear racial variation in the position of the greater palatine foramen. Therefore detailed knowledge of the population specific data on biometric features of the greater palatine foramen will facilitate therapeutic, local anesthetic and surgical manipulations in the maxillo-facial region. The goal of this study was to elucidate the morphological features and precise anatomical position of the greater palatine foramen with reference to surrounding anatomical landmarks in an adult Sri Lankan population. A total of one hundred and thirty six adult dry skulls were assessed to determine the number, shape, direction of opening of the greater palatine foramen and straight distance from it to the palatine midline, posterior margin of the hard palate and incisive fossa. The position of the greater palatine foramen was determined in relation to the maxillary molars. The results indicated that 82.35% of the greater palatine foramina had an oval outline and located in line with the long axis of the upper third molar (77.20%). The greater palatine foramen was located 15.24 mm lateral to the median sagittal plane of the hard palate and 4.51 mm anterior to the posterior border of the hard palate. In 50% of the cases the greater palatine foramen opened in an antero-medial direction. The results of the current study further highlight the racial differences in the position of the greater palatine foramen and emphasize the need for meticulous preoperative evaluation of the greater palatine foramen in patients who are candidates for maxillo-facial surgeries and regional block anesthesia.

La evidencia apoya una variación racial evidente en la posición del foramen palatino mayor. Así, el conocimiento de datos específicos de la población sobre las características biométricas de las aperturas palatinas facilitará la realización de tratamientos terapéuticos, anestésicos locales y manipulaciones quirúrgicas en la región maxilofacial. El objetivo de este estudio fue determinar las características morfológicas y la posición anatómica precisa del foramen palatino mayor con referencia a estructuras anatómicas circundantes en una población adulta de Sri Lanka. Un total de 136 cráneos secos, adultos, fueron evaluados para determinar el número, la forma, la dirección de apertura del foramen palatino mayor y la distancia recta a la línea mediana palatina, al margen posterior del paladar duro y la fosa incisiva. La posición del foramen palatino mayor se determinó en relación con los molares superiores. Los resultados indicaron que 82,35% de los forámenes palatinos mayores tenían un contorno ovalado y situado en línea con el eje largo del tercer molar superior (77,20%). El foramen palatino mayor se encontró 15,24 mm lateral del plano sagital del paladar duro y 4,51 mm por delante del margen posterior del paladar duro. En el 50% de los casos la apertura de los forámenes fue en dirección antero-medial. Los resultados señalan las diferencias raciales en la posición del foramen palatino mayor y apuntan a la necesidad de una evaluación preoperatoria minuciosa en los pacientes candidatos a cirugías maxilofaciales y anestesia de bloque regional.

Supraorbital notch/foramen in Sri Lankan skulls: morphometry and surgical relevance / Escotadura/foramen supraorbitario en cráneos de Sri Lanka: morfometría y relevancia quirúrgica

Evidence supports the ethnic and sex variation in the form and position of the supraorbital foramen. Therefore, detailed knowledge of the population specific data on biometric features of the supraorbital foramen will facilitate diagnostic, local anesthetic and surgical manipulations in the maxillo-facial region. The goal of this study was to elucidate the morphological features and precise anatomical position of the supraorbital foramen with reference to surrounding surgically encountered anatomical landmarks in an adult Sri Lankan population. A total of one hundred and eight adult dry skulls of known sex were assessed to determine the number, shape, orientation, vertical and transverse diameters of the supraorbital foramen, transverse distance from the supraorbital foramen to the nasal midline and the zygomatico-maxillary suture and the vertical distance from the supraorbital foramen to the supraorbital rim and infraorbital foramen. The position of the supraorbital foramen was determined in relation to the infraorbital foramen. Data were evaluated between sides and sex. The supraorbital notch (64.81%) was found more frequently than the supraorbital foramen (35.19%). Of the skulls investigated, 55.56% displayed bilateral supraorbital notches, whereas 20.37% had bilateral supraorbital foramina and 24.07% had a notch on one side and a foramen on the contralateral side. The incidence of multiple supraorbital foramina was 6.48%. Sex variations were observed in the relative position of supraorbital notch/foramen from nasal midline (male: 26.12±3.89; female: 24.40±2.76), temporal crest of the frontal bone (male: 32.74±3.94; female: 30.87±4.18) and infraorbital foramen (male: 44.86±3.35; female: 43.26±3.63). The modal position for the infraorbital foramen was lateral to the lateral margin of the supraorbital notch/foramen (68.52%) and supraorbital and infraorbital foramina were lying in the same sagittal plane only in 24.07% of the skulls. The results of this study highlight the racial and sex differences and emphasize the need for meticulous preoperative evaluation of the supraorbital foramen to define the optimal locations in patients who are candidates for maxillo-facial surgeries and regional block anesthesia.

La evidencia señala que existe dimorfismo étnico y sexual en la forma y posición del foramen supraorbitario. Por lo tanto, el conocimiento detallado de los datos específicos de una población sobre las características biométricas del foramen supraorbitario facilitará el diagnóstico, anestesia local y procedimientos quirúrgicos en la región maxilofacial. El objetivo fue determinar las características morfológicas y posición anatómica exacta del foramen supraorbitario con referencia a los referencias anatómicas circundantes encontradas quirúrgicamente en una población adulta de Sri Lanka. Ciento ocho cráneos adultos secos de sexo conocido se evaluaron para determinar el número, forma, orientación, diámetros vertical y transversal del foramen supraorbitario, distancia transversal desde el foramen supraorbitario a la línea mediana nasal y sutura cigomático-maxilar y distancia vertical desde el foramen supraorbitario hasta el margen supraorbitario y foramen infraorbitario. La posición del foramen supraorbitario se determinó en relación al foramen infraorbitario. Los datos fueron evaluados según lado y sexo. La incisura supraorbitaria (64,81%) se encontró con mayor frecuencia que el foramen supraorbitario (35,19%). El 55,56% de las incisuras supraorbitarias y 20,37% de los forámenes supraorbitarios fueron bilaterales; mientras que el 24,07% de las incisuras fueron unilaterales con un foramen en el lado contralateral. La incidencia de los forámenes supraorbitarios múltiples fue del 6,48%. Se observaron variaciones sexuales en la posición relativa de la incisura/foramen supraorbitario respecto a la línea mediana nasal (hombres= 26,12±3,89; mujeres: 24,40±2,76), cresta temporal del hueso frontal (hombres= 32,74±3,94; mujeres: 30,87±4,18) y foramen infraorbitario (hombres= 44,86±3,35; mujeres= 43,26±3,63). La posición modal para el foramen infraorbitario fue lateral al margen lateral de la incisura/foramen supraorbitario (68,52 %), y los forámenes supraorbitario e infraorbitario se ubicaron en el mismo plano sagital sólo en el 24,07% de los cráneos. Los resultados muestran las diferencias raciales y sexuales y enfatizan la necesidad de una evaluación preoperatoria minuciosa del foramen supraorbitario para definir su posición en pacientes que son candidatos a cirugías maxilofaciales y bloqueo anestésico regional.

A morphologically distinct Phlebotomus argentipes population from active cutaneous leishmaniasis foci in central Sri Lanka

Although the reported aetiological agent of cutaneous leishmaniasis (CL) in Sri Lanka is Leishmania donovani, the sandfly vector remains unknown. Ninety-five sandflies, 60 females and 35 males, collected in six localities in the district of Matale, central Sri Lanka, close to current active transmission foci of CL were examined for taxonomically relevant characteristics. Eleven diagnostic morphological characters for female sandflies were compared with measurements described for Indian and Sri Lankan sandflies, including the now recognised Phlebotomus argentipes sensu lato species complex. The mean morphometric measurements of collected female sandflies differed significantly from published values for P. argentipes morphospecies B, now re-identified as Phlebotomus annandalei from Delft Island and northern Sri Lanka, from recently re-identified P. argentipes s.s. sibling species and from Phlebotomus glaucus. Furthermore, analysis of underlying variation in the morphometric data through principal component analysis also illustrated differences between the population described herein and previously recognised members of the P. argentipes species complex. Collectively, these results suggest that a morphologically distinct population, perhaps most closely related to P. glaucus of the P. argentipess. I. species complex, exists in areas of active CL transmission. Thus, research is required to determine the ability of this population of flies to transmit cutaneous leishmaniasis.

Sexual differences in the diameter of coronary arteries in an adult Sri Lankan population / Diferencias sexuales en el diámetro de las arterias coronarias en una población adulta de Sri Lanka

Coronary artery disease is a major cause of human mortality. It is stated that females have worse outcomes than men following myocardial infarction and coronary revascularization. Sexual differences in the coronary artery diameters have also been speculated as one of the reasons for the above outcome. However, because of possible confounding effects, such as the body size and heart weight, it is unclear if there is a true sex-specific effect on coronary arterial size. The present study was undertaken to investigate the sexual differences in the diameter of coronary arteries in a group of adult Sri Lankan population. The diameters of the coronary arteries and their branches were measured at predetermined sites in a total of one hundred and two apparently healthy hearts obtained from cadavers during routine gross anatomy dissections. All measurements were taken using a digital sliding caliper capable of measuring to the nearest 0.01mm. The mean coronary arterial diameters were significantly smaller in females than in males. These differences persisted even after the diameters of coronary arteries were corrected for heart weight and body surface areas. Precise knowledge of the expected normal coronary arterial diameter at a given anatomic location is the first step towards developing a quantitative estimate of the severity of the coronary artery disease. This study provides a reference data set for adult Sri Lankans against which to compare the diameters of coronary arteries in various pathological conditions.

La enfermedad coronaria es la mayor causa de mortalidad humana. Se afirma que las mujeres tienen peores resultados que los hombres después de un infarto de miocardio y revascularización coronaria. Diferencias sexuales en los diámetros de las arterias coronarias han sido especuladas como una de las razones para los resultados anteriores. Sin embargo, debido a posibles efectos de confusión, tales como talla corporal y peso del corazón, no está claro si hay un verdadero efecto sexo-específico sobre el tamaño arterial coronario. El presente estudio se realizó para investigar las diferencias sexuales en el diámetro de las arterias coronarias en un grupo de adultos de la población de Sri Lanka. Los diámetros de las arterias coronarias y sus ramas se midieron en lugares predeterminados en un total de 102 corazones aparentemente sanos obtenidos de cadáveres durante las disecciones anatómicas de rutina. Todas las medidas fueron tomadas con un caliper digital deslizante (precisión 0,01 mm). La media del diámetro arterial coronario fue significativamente menor en mujeres que en hombres. Estas diferencias persisten aún después de que los diámetros de las arterias coronarias fueron corregidos por el peso del corazón y talla corporal. El conocimiento preciso del diámetro de la arteria coronaria normal esperado en un determinado lugar anatómico es el primer paso hacia el desarrollo de una estimación cuantitativa de la gravedad de la enfermedad de las arterias coronarias. Este estudio proporciona un conjunto de datos de referencia para adultos de Sri Lanka con el cual comparar los diámetros de las arterias coronarias en diversas condiciones patológicas.

Cranial Capacity in an Adult Sri Lankan Population: Sexual Dimorphism and Ethnic Diversity / Capacidad Craneal en la Población Cingalés Adulta: Dimorfismo Sexual y Diversidad Étnica

Cranial capacity exerts a close correlation with the volume of the brain. A large body of evidence shows a clear racial variation in cranial dimensions. Therefore detailed knowledge of the population specific data on biometric features of the cranial cavity is important in the study and comparison of the crania of populations from different racial backgrounds, assessing growth and development of an individual and in the diagnosis of any abnormalities of cranial size and shape. Despite its significance, little is known concerning the cranial capacity in Sri Lankans. Hence, the present study was undertaken to establish the cranial capacity in an adult Sri Lankan population. A total of 210 subjects with an age span of 20-23 years were included in the study. The cranial length, breadth and auricular head height of the subjects were recorded using a digital sliding caliper and Todd's head spanner capable of measuring to the nearest 0.01mm. The cranial capacity was calculated using external dimensions of the skulls (Lee & Pearson, 1901; Williams et al., 2000). The findings of the study indicated significant sexual differences in the mean cranial capacity (male: 1421.12 ± 171.69, female: 1300.95 ± 158.18). Positive correlations between cranial capacity and cranial dimensions were observed. Linear regression models for the prediction of cranial capacity were formulated for both sexes. The results of this study highlight the racial and sexual differences in cranial capacity in an adult Sri Lankan population. The data will be of immense use in clinical, medico-legal, anthropological and archeological studies.

La capacidad craneal indica una estrecha relación con el volumen cerebral. Existen numerosas evidencias que muestran una variacion racial en las dimensiones craneanas. Así, un conocimiento detallado de datos biométricos de la cavidad craneana en poblaciones y una comparación de cráneos en individuos de diferentes etnias permiten evaluar el crecimiento y desarrollo en un individuo y el diagnóstico de anomolías de la forma y tamaño de la cabeza. A pesar de lo anterior, no existe mucha información de la capacidad craneana en individuos cingaleses. El objetivo de este estudio fue conocer la capacidad craneana en una población adulta de Sri Lanka, para ello fueron incluidos en el estudio 210 sujetos con edades entre 20 y 23 años. La longitud craneal y ancho de la cabeza a nivel auricular fue medido con un caliper digital Todd, con una precisión de 0,01 mm. La capacidad craneana fue calculada utilizando la dimensión externa del cráneo (Lee & Pearson, 1901; Williams et al., 2000). Los resultados de este estudio indicaron diferencias sexuales significativas en la capacidad craneana media (hombre: 1421.12 ± 171.69, mujer: 1300.95 ± 158.18). Se observaron correlaciones positivas entre la capacidad craneana y las dimensiones craneanas. Fueron desarrollos modelos de regresión lineal de predicción de la capacidad craneal para ambos sexos. Los resultados indicaron diferencias tanto raciales y sexuales en la población adulta de Sri Lanka. Estos resultados serán de utilidad en los estudios clínicos, médico-legales, antropológicos y arqueológicos.