The surgical outcome of sutureless skin closures using Octyl-2-cyanoacrylate (Dermabond™) versus Steri-Strip™.

Introduction: Cosmesis is the primary concern for the patient undergoing facial surgery and there are numbers of well proven materials that are available such as adhesive tapes, subcuticular suture, skin adhesive or glue to achieve better cosmesis. The objective of our study was to assess the surgical outcome of sutureless skin closures using Octyl-2-cyanoacrylate (Dermabond™) versus Steri-Strip™. Method: The present prospective study was conducted in 20 patients. Patients were divided into two groups. After subcutaneous closure of wounds, either Dermabond™ or Steri-Strip™ was placed. The patients were assessed for wound complication (erythema, tenderness, dehiscence or any discharge), scar hypertrophy and cosmetic appearance also time consumed in surgical skin closure was evaluated. Wound assessment, scar hypertrophy and cosmetic appearance were assessed by using Chi-square test. Time closure was assessed by using Mann-whitney U test. Result: Twenty patients belonging to all age group were included in study. Ten patients undergone closure with Dermabond™ and ten with Steri-Strips™. Assessment of wound complications, cosmetic appearance and scar hypertrophy was done. There was no significant difference found between both the groups, but 2 patients had fair cosmetic outcomes at one month and 1 patient had fair scar hypertrophy at 6 month. However, excellent cosmetic outcome in terms of scar hypertrophy at 6 month was significantly more among group II. Conclusion: Octyl-2-cyanoacrylate (Dermabond™) and Steri-Strip™ provide similar outcomes in terms of wound complications. Cosmetic outcomes in terms of scar hypertrophy with steri-strip wound closure seem to be better and more economical.

Lateral flow chromatography strip system for rapid fluorescence determination of phycocyanin in water samples.

Phycocyanin (PC) is of great significance to biomedicine and water environmental safety. Hence, it is indispensable to develop facile and rapid method for PC determination. In this investigation, a system containing lateral flow chromatography (LFC) strip (which was deposited with molecularly imprinted polymer (MIP) capped CdTe quantum dots (QDs) based mesoporous structured coated silica nanoparticles, SiO2@QDs@ms-MIP NPs) and miniaturized fluorimeter was first fabricated. In detail, a two-step strategy was utilized for preparation of SiO2@QDs@ms-MIP NPs, which consisted of modification of CdTe QDs onto the silica NPs first, and synthesis of mesoporous imprinting shell by using PC as template molecule and cetyltrimethylammonium bromide (CTAB) as surfactant. After that, novel fluorescence NPs possessing specific recognition and sensitivity toward PC in seawater and lake water were acquired. The resulting fluorescent sensing system exhibited outstanding performances, which included excellent sensitivity (4.5 nmol/L), satisfactory specificity (imprinting factor, 2.31), appropriate linearity range (0.01-5 µmol/L), good recovery (96.0-101.7 %), excellent stability (relative standard deviation, RSD<1.1 %), wonderful reproducibility (RSD<1.1 %), and excellent anti-interference ability. The results of the fluorescent sensing system were superior to those of the commonly used ultraviolet (UV) method. The proposed strategy showed great potential for fast (<10 min) and convenient fluorescence detection of PC in real samples.

Time-series satellite remote sensing reveals gradually increasing war damage in the Gaza Strip.

War-related urban destruction is a significant global concern, impacting national security, social stability, people's survival and economic development. The effects of urban geomorphology and complex geological contexts during conflicts, characterized by different levels of structural damage, are not yet fully understood globally. Here we report how integrating deep learning with data from the independently developed LuoJia3-01 satellite enables near real-time detection of explosions and assessment of different building damage levels in the Israel-Palestine conflict. We found that the damage continually increased from 17 October 2023 to 2 March 2024. We found 3747 missile craters with precision positions and sizes, and timing on vital infrastructure across five governorates in the Gaza Strip on 2 March 2024, providing accurate estimates of potential unexploded ordnance locations and assisting in demining and chemical decontamination. Our findings reveal a significant increase in damage to residential and educational structures, accounting for 58.4% of the total-15.4% destroyed, 18.7% severely damaged, 11.8% moderately damaged and 12.5% slightly damaged-which exacerbates the housing crisis and potential population displacement. Additionally, there is a 34.1% decline in the cultivated area of agricultural land, posing a risk to food security. The LuoJia3-01 satellite data are crucial for impartial conflict monitoring, and our innovative methodology offers a cost-effective, scalable approach to assess future conflicts in various global contexts. These first-time findings highlight the urgent need for an immediate ceasefire to prevent further damage and support the release of hostages and subsequent reconstruction efforts.

Effect of super-high water materials backfilling on stress decrease and energy release during strip coal pillar mining: A case study.

Mining strip coal pillars left due to strip mining is important to resource-exhausted coal mines in eastern China. Backfilling mining is an effective means to mine strip coal pillars, which could decrease high stress and high energy release. However, materials with super-high water content were not widely applied in deeply isolated coal pillar mining due to lower strength characteristics and durability. In the paper, taking panel c8301 as engineering background, theoretical analysis, numerical simulation, and field monitoring were used to study the feasibility and application effect of the super-high-water backfilling technology in deep isolated coal pillar mining. The results showed that: (1) Panel c8301 is a strip-filling working face with insufficient mining on both sides, and the mining of the panel will trigger the rebalancing of the overlying rock structure on both sides, which increases the rock burst risk. (2) Simulation results indicate that the super-high-water filling method, in comparison to the traditional caving method, significantly reduces peak stress and elastic energy from 112.3 MPa to 4.6 × 106 J to 79.2 MPa and 2.23 × 106 J, respectively. This represents reductions of 29.6 % and 51.5 %, effectively mitigating the impact of mining activities on overburden movement. (3) On-site measurement data confirmed that the measured equivalent mining height was 1.25 m. The total number of microseisms and the amount of released energy decreased significantly, More specifically, three big energy release instances (>1.8 × 105 J) were recorded during the first roof weighting stage and panel in square meters. Super-high-water filling technology has achieved remarkable results in the mining of strip coal pillars and has significant application prospects.

Fentanyl and Sudden Death-A Postmortem Perspective for Diagnosing and Predicting Risk.

Sudden, unexpected deaths are extremely difficult for families, especially when the victim is a child. Most sudden deaths occur due to cardiovascular issues, and a smaller number (approximately one-quarter) are attributed to other causes, such as epilepsy. The medicinal and non-medicinal use of the synthetic opioid fentanyl, which can cause breathing problems, is frequently involved in these deaths. It is also being found more often in autopsies of sudden death cases, and the number of overdose deaths from illicit drugs containing fentanyl is increasing. There are cases in which it is mixed with other drugs. A gene known as the KCNH2 gene or human ether-a-go-go-related gene (hERG), involved in the heart's electrical activity, can be related to abnormal heart rhythms. This gene, along with others, may play a role in sudden deaths related to fentanyl use. In response, we have examined the scientific literature on genetic variations in the KCNH2 gene that can cause sudden death, the impact of fentanyl on this process, and the potential benefits of genetic testing for the victims to offer genetic counseling for their family members.

A novel peptide pair-based rapid fluorescent diagnostic system for malaria Plasmodium falciparum detection.

Advanced diagnostic materials, such as aptamers, are required due to the scarcity of efficient diagnostic antibodies and the low sensitivity of rapid diagnostic kits at detecting the malaria parasite, Plasmodium falciparum. METHODS: Two peptides M2.9 [(KPTAEQTESPELQSAPEN) and M2.17 (KILFNVYSPLGCTCECWV)] were designed using simple epitope prediction tools and modified against the merozoite surface antigen 2 of P. falciparum (Pf.MSP2) by 3-dimensional modeling based on binding affinity. Based on five prediction tools for hydropathy, M2.17 was selected as an appropriate capture peptide. A peptide-based fluorescence-linked immunosorbent assay (FLISA) and a peptide pair-based fluorescent immunochromatographic test strip (FICT) were developed to detect P. falciparum 3D7 (drug-sensitive) and P. falciparum K1 (multi drugs-resistant) strains. RESULTS: Bioinformatic analysis of two peptides demonstrated the potential binding affinity with the merozoite surface protein 2 of P. falciparum (Pf.MSP2) with a positive hydropathy value. The limit of detection (LOD) of FLISA was 10 parasites/µL and of a peptide pair-linked rapid FICT system was 5 and 200 parasites/µL for P. falciparum 3D7 and K1, respectively. Compared to commercial rapid detection systems (RDTs), a peptide pair-linked FICT system exhibited a 20-fold greater efficiency in detecting P. falciparum 3D7 and specifically discriminated another protozoan spp. CONCLUSION: A peptide pair-linked rapid diagnostic strip could be an alternative to conventional RDTs for monitoring wild-type and drug-resistant malaria parasites.

Effects of diversified volatile profiles on olfactory orientation of flea beetles Phyllotreta spp. and the diamondback moth Plutella xylostella.

This study investigated the effect of mixing volatile organic compounds (VOC) emitted by host and non-host plants on the orientation of key pests of Brassicaceae. The study aimed to understand how these mixed VOCs influence pest behaviour, which could help in tailoring pest management strategies. The orientations of flea beetles, Phyllotreta spp., and the diamondback moth (DBM), Plutella xylostella, towards cabbage VOCs mixed with faba bean VOCs were assessed using Y-tube olfactometry. The pests' preferences were measured to determine if the presence of faba bean alongside cabbage altered their olfactory orientation compared to cabbage alone. Flea beetles showed a preference for cabbage VOCs alone over the cabbage-faba bean VOC mix. For DBM, no significant preference was observed between cabbage alone and the cabbage-faba bean mix. Previous findings indicated that faba bean attracts DBM, and in this study the mixture of cabbage and faba bean appeared to be more attractive than cabbage alone. The results indicate that faba bean VOCs can deter flea beetles from cabbage, potentially offering a pest management strategy. However, the effect on DBM was inconclusive, with no clear preference observed. This suggests that while faba bean VOCs may influence pest orientation, their effectiveness varies among different pest species. Additionally, herbivore damage to cabbage leaves did not appear to influence the odour-guided orientation of either pest, irrespective of the presence or absence of faba bean.

Stability evaluation method of gasification coal pillar under thermal coupling condition for prevention of environment secondary pollution.

The instability of gasification coal pillars can easily induce the further development of water-conducting fractures, which leads to the connection of gasification combustion space and aquifer, and then causes groundwater pollution. Therefore, it is crucial to assess the stability of gasification coal pillar to forestall the potential risk of environmental contamination resulting from underground coal gasification. In this paper, based on the shape of the combustion space area of underground coal gasification, considering the influence of the mechanical properties of the coal pillar and the temperature field under the condition of thermal coupling, the calculation method of the yield zone width of gasification coal pillar is proposed. Considering the destabilizing factors affecting the coal pillar and the relationship between actual and ultimate bearing capacities after stripping and yielding, a stability evaluation method for the 'hyperbolic' coal pillar is proposed. Additionally, the effects of various factors on the stripping, yielding, and safety factor of the coal pillar are analyzed. The new method was applied to the Ulanqab underground coal gasification test site, which proved its effectiveness. The research results are of great practical significance for designing underground coal gasification production and preventing environmental pollution.

Evaluating the Success of Preformed Resin Bonded Composite Strip Crowns for the Primary Central Incisor in Restoring Carious Primary Canine Teeth: A Retrospective Analysis.

Background Restoration of primary canine teeth in pediatric dentistry requires a balance of functional, biological, and esthetic factors. Stainless steel crowns, while effective for posterior restorations, often have patient acceptance issues due to esthetic limitations. Resin-bonded composite strip crowns have gained traction for anterior restorations due to their superior esthetic qualities and repairability. However, their long-term performance in primary canines characterized by unique morphological and functional demands has not been thoroughly evaluated. This study investigates the efficacy of resin-bonded incisor strip crowns in primary canines over three years, assessing their functional durability, biological integrity, and esthetic performance to provide a comprehensive evaluation of their long-term success in this application. Methods This retrospective observational study, conducted from September 2023 to December 2023 at the Department of Pediatric and Preventive Dentistry, Saveetha Dental College and Hospitals, Chennai, Tamil Nadu, India, received ethical approval from the Institutional Human Ethical Committee (IHEC/SDC/PEDO-2103/23/131). Dental records from January 2020 to December 2020 were reviewed by two investigators to identify children aged six years or younger who had undergone pulpectomy in primary canine teeth and were restored with resin-bonded incisor strip crowns. Exclusions included records with missing contact details or post-treatment radiographs. The purpose was to ensure a three-year review period when contacting patients in 2023. Eligible parents were contacted by phone, informed about the study, and invited for a review. Informed consent was obtained in person. Children with exfoliated primary canines and those whose parents did not sign the consent form were excluded. The restored crowns were evaluated using World Dental Federation (FDI) criteria across functional, biological, and esthetic domains by two calibrated examiners. Statistical analysis was conducted using IBM SPSS Statistics for Windows, Version 24 (Released 2016; IBM Corp., Armonk, New York, United States), with categorical variables expressed as n (%), inter-examiner reliability assessed by Cohen's kappa, and chi-square test used for goodness of fit, setting significance at 95%. Results Of the 92 primary canines restored with resin-bonded incisor strip crowns, 60 children were evaluated. The retention rate at the three-year follow-up was 50 (83.3%). Functional evaluation revealed that 48 (80%) of crowns maintained surface texture, 42 (70%) preserved form and contour, and 52 (86.6%) demonstrated acceptable occlusion and wear. Biologically, 55 (91.7%) of the crowns had no secondary caries, and 56 (93.4%) had no dental hard tissue defects at the margin. Esthetically, 48 (80%) of crowns retained surface texture, 46 (76.6%) had a satisfactory color match, and 45 (71.6%) exhibited minimal marginal staining. Statistical analysis confirmed significant results for all functional and esthetic parameters (p < 0.05). Conclusion The technique of placing incisor strip crowns in the place of primary canines can be recommended considering its maintenance of functional, biological, and esthetic properties.

A microfluidic paper-based fluorescent sensor integrated with a smartphone platform for rapid on-site detection of omethoate pesticide.

A novel approach combing a fluorescent microfluidic paper strip with a portable smartphone-based sensing platform is developed for rapid and sensitive detection of omethoate pesticide. The detection mechanism of the microfluidic paper strip is based on the fluorescence quenching of graphene oxide (GO) toward the cyanine 3 (Cy3)-labeled aptamer (Cy3-APT). Upon exposure to omethoate, the Cy3-APT detaches from the surface of GO, resulting in considerable fluorescence recovery, which can be visualized through the smartphone-based sensing platform. The images are analyzed through a self-developed app embedded with a pretrained convolutional neural network model, achieving a high regression coefficient of 0.9964 at an omethoate concentration range of 0-750 nM. The smartphone-based platform enables rapid on-site detection of omethoate pesticide in real samples within 10 min, with results comparable to those obtained using standard methods. In short, the proposed microfluidic paper-based fluorescent sensor combined with the smartphone-based sensing platform enhances the detection performance toward organophosphorus pesticides.

Validation of behavioral measures of social cognition in individuals diagnosed with schizophrenia.

Schizophrenia, a complex neuropsychiatric disorder, manifests severe impairments in social cognition, notably in Theory of Mind (ToM), empathy, and emotion recognition, which significantly influence social competence and overall functioning. These aspects are crucial for prognosis in individuals diagnosed with schizophrenia (SZ). This study validates a comics strip paradigm for ToM and empathy assessment, the Montreal Affective Voices (MAV) for measuring emotion recognition, and a Go-NoGo task for inhibition control estimation in individuals diagnosed with SZ, comparing their performance with healthy controls. SZ participants exhibited diminished abilities in the comics strip task, especially in ToM and empathy conditions, alongside challenges in identifying emotions from vocal cues in MAV. They responded slower and tended to be less accurate in the Go-NoGo task. The validated behavioral battery addresses the limitations of previous measures and emerges as a promising tool for future investigations into the neural systems underlying social cognition in schizophrenia. Such insights can lead to the development of long-needed treatment for negative symptoms and social dysfunctions in schizophrenia.

Rapid and equipment-free identification of papaya mealybug Paracoccus marginatus based on RPA-CRISPR/Cas12a.

BACKGROUND: Paracoccus marginatus has invaded many countries, spreading rapidly and causing significant economic losses to crops. Accurate detection during the monitoring process is critical to prevent its expansion into new areas, therefore it is necessary to develop efficient and reliable detection methods. Traditional detection methods are time-consuming and instrument-dependent owing to the morphological similarities and small sizes of P. marginatus and other mealybugs, therefore establishing an efficient, rapid, and sensitive method for field detection in resource-limited settings is critical. RESULTS: A sensitive and rapid detection system was developed to detect P. marginatus using recombinase polymerase amplification (RPA) combined with clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a. The RPA-CRISPR/Cas12a assay distinguished P. marginatus from 10 other mealybugs. The entire process can be completed in approximately an hour, and the identification results can be determined by the naked eye using lateral flow strips or a portable mini-UV torch. A method was developed to extract DNA from P. marginatus within 5 min. This method was combined with the RPA-CRISPR/Cas12a assay to achieve rapid and simple detection. In addition, two portable thermos cups with temperature displays were used to maintain the reagents and assay reactions in the field. CONCLUSION: This assay represents the first application of portable and easily available items (mini-UV torch and thermos cup) based on the combination of RPA and CRISPR/Cas12a for rapid pest detection. This method is rapid, highly specific, and instrument-flexible, allowing for the early monitoring of P. marginatus in the field. This study provides guidance for the development of suitable management strategies. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Establishment of highly sensitive lateral flow immunochromatographic strips for quinclorac detection utilizing signal amplification nanoparticles.

Highly selective herbicide quinclorac (Qui) is a type of quinoline carboxylic acid hormone herbicide, which has the characteristics of long half-life and difficulty for degradation, causing high risk to the environmental safety. In this study, anti-Qui 8A3 monoclonal antibody (mAb) with good specificity and high affinity (3.89 × 109 L/mol) was prepared, and two kinds of lateral flow immunochromatographic strips (LFICS) including nano-flower nanoparticles (AuNF) - and latex microsphere (LM)- based LFICS were established based on the antibody and signal amplification. The linear range of the AuNF- and LM- based LFICS were 5.31-345.48 ng/mL and 2.52-257.92 ng/mL, respectively. The limit of detection (LOD) of the AuNF- and LM- based LFICS were determined to be 5.31 ng/mL and 2.52 ng/mL, respectively. In summary, the developed LFICS using AuNF and LM as signal amplification reporters exhibited excellent sensitivity and provided the rapid on-site screening of Qui and other analytes in food safety field.

Rapid lateral flow test for Mycobacterium tuberculosis complex and non-tuberculous mycobacteria differentiation.

The diagnosis of mycobacterial infections, including both the Mycobacterium tuberculosis complex (MTBC) and non-tuberculous mycobacteria (NTM), poses a significant global medical challenge. This study proposes a novel approach using immunochromatographic (IC) strip tests for the simultaneous detection of MTBC and NTM. Traditional methods for identifying mycobacteria, such as culture techniques, are hindered by delays in distinguishing between MTBC and NTM, which can affect patient care and disease control. Molecular methods, while sensitive, are resource-intensive and unable to differentiate between live and dead bacteria. In this research, we developed unique monoclonal antibodies (mAbs) against Ag85B, a mycobacterial secretory protein, and successfully implemented IC strip tests named 8B and 9B. These strips demonstrated high concordance rates with conventional methods for detecting MTBC, with positivity rates of 93.9% and 85.9%, respectively. For NTM detection, the IC strip tests achieved a 63.2% detection rate compared to culture methods, considering variations in growth rates among different NTM species. Furthermore, this study highlights a significant finding regarding the potential of MPT64 and Ag85B proteins as markers for MTBC detection. In conclusion, our breakthrough method enables rapid and accurate detection of both MTBC and NTM bacteria within the BACTEC MGIT system. This approach represents a valuable tool in clinical settings for distinguishing between MTBC and NTM infections, thereby enhancing the management and control of mycobacterial diseases. KEY POINTS: • Panel of mAbs for differentiating MTB versus NTM • IC strips for diagnosing MTBC and NTM after the BACTEC MGIT • Combined detection of MTP64 and Ag85B enhances diagnostic accuracy.

Edema and Trismus Assessment After Applying Kinesiotape With the Web Strip Technique Compared With Cryotherapy: A Randomized Clinical Trial.

BACKGROUND AND OBJECTIVES: Extraction of impacted third molars is one of the most common oral surgeries performed in the dental clinic, which is often accompanied by many complications such as edema and trismus. Many methods have been used to alleviate these complications, such as drugs or physical therapy. Kinesiotape (KT) has recently spread as a popular physical method for eliminating complications after surgical extraction of lower third molars after its long-term use in sports medicine and injuries of the musculoskeletal system. The current study aimed to study the effect of using KT (Kinesio® Holding Corporation, Albuquerque, NM, USA) on both edema and trismus after impacted third molar extraction. METHODOLOGY: This study was designed as a randomized controlled clinical trial using the split-mouth technique and included 25 patients with radiographically symmetrical lower third molars from patients. All surgical extractions were performed by a single surgeon under sterile conditions according to the standard surgical protocol after that one group applied KT and the other group applied cryotherapy. Edema and trismus were measured in the first five days. Data was collected and analyzed by SPSS software (IBM Corp., Armonk, NY, USA). RESULTS: The mean change in the total linear facial measurements in the experimental group (Kinesio Group) was 12.32 mm three days after surgery, and then this value decreased to 6.80 mm, while the average increase in the control group (Cryo Group) was 17.00 mm after three days, then the value decreased to 9.68 mm five days after surgery. Regarding the changes in the amount of maximum mouth opening after surgery, the results were similar between the Kinesio Group and the Cryo Group, as there were no significant differences between the two groups (P<0.05). CONCLUSION: This study concluded that KT was superior to cryotherapy when studying edema. The current study also concluded that the mouth opening was similar between the two study groups.

Chromogenic Chemosensor for Simultaneous Detection of PO43¯ and CO32¯ Anions in Organo-Aqueous Solutions: Application in Arduino Based Electronic Color Sensor Device and Logic Gate.

Three new chromogenic receptors have been synthesized with the primary objective of facilitating the selective recognition of PO43¯and CO32¯ ions in an organo-aqueous medium. R1 and R2 exhibit an extraordinary detection limit aligning with both EPA and WHO guidelines. R1 shows LOD of 0.135 ppm for PO43¯ and 0.175 ppm for CO32¯, while R2 sets forth a LOD of 0.427 ppm for PO43¯ and 0.729 ppm for CO32¯. The binding mechanism involves intramolecular charge transfer (ICT) band are substantiated by comprehensive studies that include UV-Vis titration, 1H-NMR titration, DFT studies and electrochemical studies. Chemosensors were employed in the formulation of logic gate, the fabrication of a paper strip test kit and its application in RGB color sensor device.

Development of a new accurate lateral flow immunoassay for diagnosis of human leptospirosis.

PURPOSE: The current diagnostic methods for leptospirosis diagnosis are technically complex and expensive, with limited applicability to specialized laboratories. Furthermore, they lack diagnostic accuracy in the acute stage of the disease, which coincides with a period when antibiotics are highly effective. New simple and accurate tests are mandatory to decentralize and improve diagnosis. Here, we introduced a new lateral flow immunoassay (Lepto-LF) for human leptospirosis. METHODS: We conducted a double-blinded assay using 104 serum samples from patients with confirmed or discarded diagnosis for leptospirosis. The diagnostic performance of Lepto-LF was estimated across different ranges of days from onset of symptoms (dpo), considering the diagnostic algorithm as reference standard. Additionally, it was compared with the screening methods enzyme-linked immunosorbent assay (IgM-ELISA) and the slide agglutination test using temperature-resistant antigen (SATR). RESULTS: Lepto-LF exhibited perfect diagnostic performance with a Youden´s index J = 1 from 6 dpo in the acute phase. IgM-ELISA gave slightly lower accuracy with J = 0.91 and 95.5% of both sensitivity and specificity; while SATR showed a markedly inferior yield (J = 0.41, sensitivity = 95.5%, specificity = 45.5%). The performances remained consistent in the convalescence phase of the disease (> 10 dpo). CONCLUSION: Lepto-LF was found to be a reliable test for simple, rapid and early diagnosis of leptospirosis, resulting a promising tool for decentralizing leptospirosis diagnosis and enabling timely treatment of patients. In addition, Lepto-LF may be employed as confirmatory test, especially in remote areas and vulnerable contexts where the standard MAT is not available.

Development of quantum dot-based immunochromatographic strip for detection of antibodies against ASFV pp62.

ASFV is the only known double-stranded insect-borne DNA virus, which can rapidly infect domestic pigs and wild boars with ticks as transmission medium. Since it was first discovered in 1921, it quickly spread to all parts of the world and brought huge economic losses to the pig industry all over the world. At present, there is still no safe and effective vaccine for ASFV. Here, we developed a quantum-dot labeled antibody test strip for the detection of antibodies against ASFV pp62. The pp62 protein was labeled with quantum dots, and the antibody test strip was developed uses it in a detection mode of labeled antigen-SPA interceptor-monoclonal antibody quality control. The test strip showed high sensitivity, the positive detection limit of the strip was 1: 106 by continuous multiple dilution using the positive standard serum of ASFV antibody as reference. The test strip showed good specificity, and there was no cross reaction with other swine diseases virus (PCV2, PRRSV, CSFV, PPV). Using the detection results of commercialized kit for African swine fever virus as reference, 80 ASFV antibody negative serum and 4 different ASFV antibody positive serum were detected using the ASFV pp62 quantum-dot labeled antibody test strip. The results were consistent with the commercial kit. This study provides a new detection method for the prevention and control of African swine fever.

Occupational stress and associated sources and risk factors among nurses in Gaza strip, Palestine: A cross-sectional survey.

AIM: To determine the occurrence of occupational stress among Palestinian nurses, and their associated sources and risk factors. DESIGN: A cross-sectional descriptive design. METHODS: A total of 250 registered nurses from eight governmental hospitals, using a convenience sampling method. Data collection were conducted using the 30-items self-reported Occupational Stress Scale from December 2022 to March 2023. Descriptive statistics, independent sample t-test, one-way ANOVA, and multiple linear regression analysis were applied to analysis data. Data analysis included descriptive statistics, independent sample t-test, one-way ANOVA, and multiple linear regression analysis. RESULTS: The prevalence of high occupational stress levels was 64.8% (Mean = 3.9 out of 5). The main sources of stress are too much responsibility and work, understaffing, lack of promotion and recognition, inadequate pay, time pressure, and management style. The results regression analysis demonstrated that male nurses with a Masters or PhD degree and those working in fixed shifts experienced higher occupational stress. Moreover, participants who worked overtime hours were more susceptible to stress. CONCLUSIONS: The research indicates that occupational stress presents a notable challenge for nurses in the Gaza Strip, Palestine. It suggests that in order to alleviate this stress, decision-makers in healthcare policy and hospital management should prioritize the execution of strategies aimed at addressing the primary stressors and risk factors identified. REPORTING METHOD: This study adhered to the STROBE guidelines. PUBLIC CONTRIBUTION: A total of 250 registered nurses were taken part in this study by answering a self-administered study survey.

Rapid Detection of Getah Virus Antibodies in Horses Using a Recombinant E2 Protein-Based Immunochromatographic Strip.

The prevalence and impact of Getah virus (GETV) are significant concerns in China. GETV can infect a wide range of animals, including horses, pigs, sheep, cattle, birds, and humans, resulting in substantial losses in the livestock and agricultural industries. GETV infection can cause the development of ulcers and inflammation in the mouth and gums of horses, which result in pain and discomfort and lead to symptoms such as reduced appetite, drooling, and difficulty chewing. As a result, there is a pressing need for efficient and rapid disease diagnosis methods. However, the currently available diagnostic methods have limitations in terms of operational time, equipment, and the experience of the individuals using them. In this study, a rapid, specific, and sensitive detection method was developed using a colloidal gold-based immunochromatographic strip (ICS) for the detection of antibodies against GETV in horses. To prepare the ICS, the antigen domain of the E2 glycoprotein of GETV was expressed using the Escherichia coli expression system after analysis with DNAstar v7.1 software. The nitrocellulose membrane was coated with rE2 protein or SPA to form the test line and control line, respectively. After optimizing the reaction conditions, the sensitivity, specificity, and repeatability of the strip were verified. The results showed that the test strip had a detection limit of up to 1:320 dilutions for GETV-positive serum, with no cross-reactivity observed with other equine-susceptible pathogens such as equine arteritis virus (EAV), equine herpesvirus-1 (EHV-I), equine infectious anemia virus (EIAV), equine influenza virus (EIV), African horse sickness virus (AHSV), and Japanese encephalitis virus (JEV). Furthermore, the ICS exhibited a concordance rate of 94.0% when testing 182 clinical serum samples compared to the virus neutralization test. Overall, this ICS diagnosis method will be an effective tool for the rapid detection of GETV in the field.