An eco-friendly enzymatic treatment to prepare spinnable banana fibers as an alternative to cotton for textile applications.

Banana fibers are a sustainable material with natural mechanical strength and antibacterial properties. These fibers are extracted from the large amount of waste produced by banana pseudo stems annually. However, despite their numerous advantages, their stiffness and rough texture impede their full use in the textile. This research investigates the degumming treatment of banana fibers using enzyme combination and chemical methods to achieve spinnable soft banana fibers. An L9 orthogonal array was used in a Taguchi design of the experiment to optimize the process parameters. For enzyme combination degumming, the experimental setup comprised different quantities of hemicellulase, laccase, amylase, and pectinase; for chemical degumming, varied amounts of sodium hydroxide (NaOH) were used. The results indicate that enzyme-based degumming procedures produce better results than chemical treatments. Optimum enzyme combinations for various fiber qualities were found using the Taguchi design of experiments. These combinations included Hemicellulase 5 %, Laccase 5 %, Amylase 3 %, and Hemicellulase 5 %, Laccase 3 %, Pectinase 5 %. Without degrading the cellulose structure, these ideal enzyme combinations produced fibers with lower lignin content and higher cellulose percentages, moisture content, and tenacity values. By determining the most efficient enzyme combinations and their effects on fiber qualities, the study offers sustainable fiber processing methods for textile grade banana fiber.

Optimization of Enterocin Production from Probiotic Enterococcus faecium Using Taguchi Experimental Design.

Objectives: Enterocin is a significant broad-spectrum peptide antibiotic produced by Enterococcus faecium (E. faecium). Enterocin production by E. faecium was investigated using the Taguchi experimental design. The Taguchi models were used to save the time and effort required for optimizing the different conditions affecting its production. They were applied to optimize the conditions for enterocin production using the least number of experiments and the least number of required materials. Materials and Methods: Seven factors i.e., pH, temperature, time of incubation, aeration rate, inoculum size, carbohydrate concentration, and bile salt concentrations, each at three levels were selected and an orthogonal array layout of L273 was performed. Results: The experimental results indicated that the best incubation conditions were; 48 hours incubation on a nutrient medium at pH 6.5, temperature at 25 °C, aeration rate at 0 round per minute, inoculum size 20 mL, and bile salt concentration. It was 5%, and the carbon concentration was 2.0%. All these factors combined led to the best enterocin production by E. faecium. Conclusion: This optimization of enterocin production by the Taguchi experimental models emphasized some important results regarding the interaction of the different driving factors leading to the best enterocin production in one experiment.

Alkaline-Tolerant Bacillus cereus 12GS: A Promising Polyhydroxybutyrate (PHB) Producer Isolated from the North of Mexico.

Environmental pollution caused by petroleum-derived plastics continues to increase annually. Consequently, current research is interested in the search for eco-friendly bacterial polymers. The importance of Bacillus bacteria as producers of polyhydroxyalkanoates (PHAs) has been recognized because of their physiological and genetic qualities. In this study, twenty strains of Bacillus genus PHA producers were isolated. Production was initially evaluated qualitatively to screen the strains, and subsequently, the strain B12 or Bacillus sp. 12GS, with the highest production, was selected through liquid fermentation. Biochemical and molecular identification revealed it as a novel isolate of Bacillus cereus. Production optimization was carried out using the Taguchi methodology, determining the optimal parameters as 30 °C, pH 8, 150 rpm, and 4% inoculum, resulting in 87% and 1.91 g/L of polyhydroxybutyrate (PHB). Kinetic studies demonstrated a higher production within 48 h. The produced biopolymer was analyzed using Fourier-transform infrared spectroscopy (FTIR), confirming the production of short-chain-length (scl) polyhydroxyalkanoate, named PHB, and differential scanning calorimetry (DSC) analysis revealed thermal properties, making it a promising material for various applications. The novel B. cereus isolate exhibited a high %PHB, emphasizing the importance of bioprospecting, study, and characterization for strains with biotechnological potential.

Central composite design augmented quality-by-design-based systematic formulation of erlotinib hydrochloride-loaded chitosan-poly (lactic-co-glycolic acid) nanoparticles.

Aim: This study aimed to formulate erlotinib hydrochloride (ERT-HCL)-loaded chitosan (CS) and poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) using Quality-by-Design (QbD) to optimize critical quality attributes (CQAs). Materials & methods: Quality target product profile (QTPP) and CQAs were initially established. Based on L8-Taguchi screening and risk assessments, central composite design (CCD) design was used to optimize NPs. Results: ERT-HCL-loaded CS-PLGA NPs had a mean particle diameter, zeta potential and entrapment efficiency of 226.50 ± 1.62 d.nm, 27.66 ± 0.64 mV and 78.93 ± 1.94 %w/w, respectively. The NPs exhibited homogenous spherical morphology and sustained release for 72 h. Conclusion: Using systematic QbD approach, ERT-HCL was encapsulated in CS-PLGA NPs, optimizing CQAs. These findings propel future research for improved NSCLC treatment.

Innovative erlotinib-loaded chitosan-PLGA nanoparticles, developed through a systematic QbD approach, promise enhanced drug delivery for NSCLC. Optimized for size, potential and entrapment efficiency, these particles demonstrate sustained release over 72 h. #DrugDelivery #QBD #NSCLC.

Optimizing tensile strength and energy consumption for FDM through Mixed-Integer Nonlinear Multi-objective optimization and design of experiments.

This study presents a methodology for optimizing key parameters of a fused deposition modeling (FDM) printer to minimize energy consumption (EC) while exceeding a specified tensile strength (TS) threshold. Employing Design of Experiments (DoE) with Taguchi and Response Surface analysis, we identify influential parameters affecting TS and EC. A Mixed-Integer Nonlinear Multi-Objective Optimization model is then utilized to balance TS and EC, resulting in optimal parameter values. Validation using fabricated specimens demonstrates less than 5 % error in Tensile Strength and less than 2 % error in Energy Consumption, confirming the efficacy of the proposed methodology.

Purification, functional characterization and enhanced production of serratiopeptidase from Serratia marcescens MES-4: An endophyte isolated from Morus rubra.

Serratiopeptidase, a proteolytic enzyme serves as an important anti-inflammatory and analgesic medication. Present study reports the production and purification of extracellular serratiopeptidase from an endophyte, Serratia marcescens MES-4, isolated from Morus rubra. Purification of the enzyme by Ion exchange chromatography led to the specific activity of 13,030 U/mg protein of serratiopeptidase, showcasing about 3.1 fold enhanced activity. The catalytic domain of the purified serratiopeptidase, composed of Zn coordinated with three histidine residues (His 209, His 213, and His 219), along with glutamate (Glu 210) and tyrosine (Tyr 249). The molecular mass, as determined by SDS-PAGE was ∼51 kDa. The purified serratiopeptidase displayed optimal activity at pH 9.0, temperature 50°C. Kinetic studies revealed Vmax and Km values of 33,333 U/mL and 1.66 mg/mL, respectively. Further, optimized conditions for the production of serratiopeptidase by Taguchi design led to the productivity of 87 U/mL/h with 87.9 fold enhanced production as compared to the previous conditions.

Process Parameter Optimization for CO2 Laser Polishing of Fused Silica Using the Taguchi Method.

Fused silica was polished to a high quality by a CO2 laser beam with a rapid scanning rate. The rapid scanning rate produced a line laser heat source, resulting in a "polishing line" during the polishing process. The Taguchi method was used to evaluate the comprehensive influence of polishing process parameters on the polishing qualities. Four factors, namely the length of laser reciprocating scanning (A), laser beam scanning speed (B), feed speed (C), and defocusing amount (D), were investigated in this study. The optimal process parameter combination (A1B1C1D1) was obtained. The surface roughness of fused silica was reduced from Ra = 0.157 µm to 0.005 µm. Through analysis of variance (ANOVA), it was found that laser beam scanning speed (B) had a significant influence on the polishing quality. The interaction of the two factors plays a decisive role in the determination of the best process parameters, and the influence of other multi-factor interaction can be ignored; the interaction between A × B is the largest, with a contribution of 42.69%.

Facile encapsulation of cyanoacrylate-based bioadhesive by electrospray method and investigation of the process parameters.

Polymer microcapsules containing cyanoacrylates have represented a promising option to develop self-healing biomaterials. This study aims to develop an electrospray method for the preparation of capsules using poly(methyl methacrylate) (PMMA) as the encapsulant and ethyl 2-cyanoacrylate (EC) as the encapsulate. It also aims to study the effect of the electrospray process parameters on the size and morphology of the capsules. The capsules were characterized using Fourier-transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and field-emission scanning electron microscopy (FE-SEM). Moreover, the effects of electrospray process parameters on the size were investigated by Taguchi experimental design. FTIR and TGA approved the presence of both PMMA and EC without further reaction. FE-SEM micrograph demonstrated that an appropriate choice of solvents, utilizing an appropriate PMMA:EC ratio and sufficient PMMA concentration are critical factors to produce capsules dominantly with an intact and spherical morphology. Utilizing various flow rates (0.3-0.5 ml/h) and applied voltage (18-26 kV), capsules were obtained with a 600-1000 nm size range. At constantly applied voltages, the increase in flow rate increased the capsule size up to 40% (ANOVA, p ≤ 0.05), while at constant flow rates, the increase in applied voltage reduced the average capsule size by 3.4-26% (ANOVA, p ≤ 0.05). The results from the Taguchi design represented the significance of solution flow rate, applied voltage, and solution concentration. It was shown that the most effective parameter on the size of capsules is flow rate. This research demonstrated that electrospray can be utilized as a convenient method for the preparation of sub-micron PMMA capsules containing EC. Furthermore, the morphology of the capsules is dominated by solvents, PMMA concentration, and PMMA:EC ratio, while the average size of the capsules can be altered by adjusting the flow rate and applied voltage of the electrospray process.

Coproduction of inulinase and invertase by Galactomyces geotrichum in whey-based medium and evaluation of additional nutrients.

The purpose of this research was to evaluate the suitability of whey as an effective medium for the coproduction of inulinase and invertase by an oleaginous yeast Galactomyces geotrichum and to investigate the effects of some additional carbon and nitrogen sources. The nutritional factors and composition of the medium have a great impact on the production pathways of microbial enzymes. To deepen the research, a Taguchi design was employed to quickly scan the best conditions. First, the cheese whey was partly deproteinized and investigated as the sole medium for the yeast. The next step was performed to study the effects of inulin, sucrose and lactose as carbon sources and ammonium sulfate, yeast extract and casein as nitrogen sources. All analyses (Taguchi and ANOVA) were performed using Minitab software. Whey-based medium without any additional carbon and nitrogen sources gave inulinase and invertase activities as 54.6 U/mL and 47.4 U/mL, respectively. Maximum inulinase activity was obtained as 77.9 U/mL using inulin as the carbon source without any nitrogen source. The highest I/S ratio was found as 2.08. On the other hand, the highest invertase activity was carried out as 50.85 U/mL in whey-based medium using lactose as carbon source without any additional nitrogen source. This is the first report about partly deproteinized whey-based medium utilization for simultaneous inulinase and invertase production by G. geotrichum TS-61. Moreover, the effects of carbon and nitrogen sources were investigated in detail.

Whey is a sufficient medium for inulinase and invertase productionInulin is an excellent carbon source for enhanced inulinase activityTaguchi orthogonal array presents an effective and quick screening method for the fermentation process.

Mixed food waste valorization using a thermostable glucoamylase enzyme produced by a newly isolated filamentous fungus: A sustainable biorefinery approach.

Food waste is a lucrative source of complex nutrients, which can be transformed into a multitude of bioproducts by the aid of microbial cell factories. The current study emphasizes isolating Glucoamylase enzyme (GA) producing strains that can effectively break down mixed food waste (MW), which serves as a substrate for biomanufacturing. The screening procedure relied heavily on the growth of isolated fungi on starch agar media, to specifically identify the microbes with the highest starch hydrolysis potential. A strain displayed the highest GA activity of 2.9 ± 0.14 U/ml which was selected and identified as Aspergillus fumigatus via molecular methods of identification. Exposure of the A. fumigatus with 200 mM Ethyl methanesulphonate (EMS) led to a 23.79% increase compared to the wild-type GA. The growth conditions like cultivation temperature or the number of spores in the inoculum were investigated. Further, maximum GA activity was exhibited at pH 5, 55 °C, and at 5 mM Ca2+ concentration. The GA showed thermostability, retaining activity even after long periods of exposure to temperatures as high as 95 °C. The improvement of hydrolysis of MW was achieved by Taguchi design where a maximum yield of 0.57 g g-1 glucose was obtained in the hydrolysate. This study puts forth the possibility that mixed food waste, despite containing spices and other microbial growth-inhibitory substances, can be efficiently hydrolyzed to release glucose units, by robust fungal cell factories. The glucose released can then be utilized as a carbon source for the production of value-added products.

Formulation and Optimization of Solid Lipid Nanoparticle-based Gel for Dermal Delivery of Linezolid using Taguchi Design.

BACKGROUND: Linezolid (LNZ) is a synthetic oxazolidinone antibiotic approved for the treatment of uncomplicated and complicated skin and soft tissue infections caused by gram-positive bacteria. Typically, LNZ is administered orally or intravenously in most cases. However, prolonged therapy is associated with various side effects and lifethreatening complications. Cutaneous application of LNZ will assist in reducing the dose, hence minimizing the unwanted side/adverse effects associated with oral administration. Dermal delivery provides an alternative route of administration, facilitating a local and sustained concentration of the antimicrobial at the site of infection. OBJECTIVE: The current research work aimed to formulate solid lipid nanoparticles (SLNs) based gel for dermal delivery of LNZ in the management of uncomplicated skin and soft tissue infections to maximise its benefits and minimise the side effects. METHODS: SLNs were prepared by high-shear homogenisation and ultrasound method using Dynasan 114 as solid lipid and Pluronic F-68 as surfactant. The effect of surfactant concentration, drug-to-lipid ratio, and sonication time was investigated on particle size, zeta potential, and entrapment efficiency using the Taguchi design. The main effect plot of means and signal-to-noise ratio were generated to determine the optimized formulation. The optimized batch was formulated into a gel, and ex vivo permeation study, in vitro and in vivo antibacterial activity were conducted. RESULTS: The optimised process parameters to achieve results were 2% surfactant concentration, a drug-to-lipid ratio of 1:2, and 360 s of sonication time. The optimized batch was 206.3± 0.17nm in size with a surface charge of -24.4± 4.67mV and entrapment efficiency of 80.90 ± 0.45%. SLN-based gel demonstrated anomalous transport with an 85.43% in vitro drug release. The gel showed a 5.03 ± 0.15 cm zone of inhibition while evaluated for in vitro antibacterial activity against Staphylococcus aureus. Ex vivo skin permeation studies demonstrated 20.308% drug permeation and 54.96% cutaneous deposition. In-vivo results showed a significant reduction in colony-forming units in the group treated with LNZ SLN-based gel. CONCLUSION: Ex vivo studies ascertain the presence of the drug at the desired site and improve therapy. In vivo results demonstrated the ability of SLN-based gel to significantly reduce the number of bacteria in the stripped infection model. The utilization of SLN as an LNZ carrier holds significant promise in dermal delivery.

A Taguchi-STIRPAT input-output model for unveiling the pathways of reducing household carbon emissions under dual-carbon target-A case study of Fujian province.

This study develops a novel Taguchi-STIRPAT input-output (TSIO) model for exploring pathways to reduce carbon emission from the perspective of household consumption, through incorporating input-output model (IOM), Taguchi design (TD), and STIRPAT model. TSIO can not only identify the main factors (carbon emission intensity, consumption structure, per capita consumption, and population) and evaluate their effects on indirect household carbon emissions (IHC), but also predict IHC from a long-term perspective to achieve the dual-carbon target. TSIO is then applied to Fujian province (China), where multiple scenarios related to multiple factors with multiple levels are examined. Results reveal that (i) among all sectors, the highest contributors to IHC are residence (RES), followed by food, cigarettes, and drinks (FCD), and transport and communication (TC); it is suggested that the government can consider market mechanism to control these high-carbon emission consumption behaviors; (ii) the decline in the share of RES consumption has the largest effect on rural and urban IHC; the share of RES consumption is considered to be a key factor in predicting carbon emissions; (iii) under the optimal scenario, IHC would peak in 2025 and decrease to 10.07 × 106 ton in 2060; this scenario can effectively mitigate household carbon emissions by reducing carbon emission intensity and the share of RES consumption; at the same time, it can ensure a sustained increase in per capita consumption. The results unveil the pathways of household carbon reduction under the dual-carbon target in Fujian province and suggest the local government should adopt policies (such as taxation and financial incentives) to limit residential consumptions with high carbon emission intensity.

A novel combined system for efficient nitrate removal using a continuous flow electrocoagulation and sand filtration (FECF) reactor: Statistical analysis by Taguchi design.

In this study, a new hybrid bench-scale electrocoagulation-sand filtration (FECF) reactor was developed for purifying nitrate-contaminated samples. Before and after electrochemical treatment, two sand filters were included in this continuous system to facilitate the purification procedure, and the contaminated water flows horizontally through the entire system according to a specific hydraulic gradient within the reactor, resulting in water purification. Significant improvement in treatment performance was observed due to the presence of metal hydroxides in the second filter media that were not fully involved in the electrocoagulation treatment. Energy dispersive X-ray (EDX) analysis was performed to detect metal hydroxide species in the sand media, and the need for filter regeneration was evaluated by monitoring changes in the system flow rate. Moreover, an evaluation of the effects of different factors including operating time, current intensity, initial pH, type of anode and cathode, initial nitrate concentration, hydraulic head level inside the reactor, number of electrodes, and NaCl electrolyte concentration on the performance of nitrate removal was conducted through the Taguchi design. Further, ANOVA analysis verified the accuracy of the predicted model, and the variables were classified based on their relative importance in the FECF process. According to the regression model, 97% of nitrates were removed with Al electrodes as anode and Fe as cathode, 70 min purification time, current intensity of 3 A, 100 mg/l initial nitrate concentration, pH 8, electrolyte concentration of 1 g/l, electrode number of 6, and 1.5 cm head level.

Adsorption of Cd(II) ions on magnetic graphene oxide/cellulose modified with ß-cyclodextrin: Analytical interpretation via statistical physics modeling and fractal like kinetic approach.

In the present study, ß-cyclodextrin modified magnetic graphene oxide/cellulose (CN/IGO/Cel) was fabricated for removal of Cd(II) ions. The material was characterized through various analytical techniques like FTIR, XRD, TGA/DTA, SEM, TEM, and XPS. The point of zero charge of the material was obtained as 5.38. The controllable factors were optimized by Taguchi design and optimum values were: adsorbent dose-16 mg, equilibrium time-40 min, and initial concentration of Cd(II) ions-40 mg/L. The material shows high adsorption capacity (303.98 mg/g). The good fitting of Langmuir model to adsorption data (R2 = 0.9918-0.9936) revealed the monolayer coverage on adsorbent surface. Statistical physics model M 2 showed best fitting to adsorption data (R2 > 0.997), suggesting the binding of Cd(II) ions occurred on two different receptor sites (n). Stereographically n > 1 confirming vertical multi-molecular mechanisms of Cd(II) ions adsorption on CN/IGO/Cel surface. The adsorption energies (E1 = 23.71-28.95 kJ/mol; E2 = 22.69-29.38 kJ/mol) concluded the involvement of physical forces for Cd(II) ions adsorption. Kinetic data fitted well to fractal-like pseudo first-order model (R2 > 0.9952), concluding the adsorption of Cd(II) ions occurred on energetically heterogeneous surface. The kinetic analysis shows that both the film-diffusion and pore-diffusion were responsible for Cd(II) ions uptake. XPS analysis was utilized to explain the adsorption mechanism of Cd(II) ions onto CN/IGO/Cel.

Statistical Modeling and Optimization of Electrospinning for Improved Morphology and Enhanced ß-Phase in Polyvinylidene Fluoride Nanofibers.

The fabrication of PVDF-based nanofiber mats with enhanced ß-phase using electrospinning and post processing was optimized using Taguchi design methodology. The parameters studied include the concentration of PVDF in the DMF (Dimethylformamide) solvent, applied voltage, flow rate, and drum speed. A reliable statistical model was obtained for the fabrication of bead-free PVDF nanofibers with a high fraction of ß-phase (F(ß)%). The validity of this model was verified through comprehensive regression analysis. The optimized electrospinning parameters were determined to be a 23 wt% PVDF solution, 20 kV voltage, a flow rate of 1 mL/h, and a drum speed of 1200 revolutions per minute.

Bio-sorptive remediation of crude oil polluted sea water using plantain (Musa parasidiaca) leaves as bio-based sorbent: Parametric optimization by Taguchi technique, equilibrium isotherm and kinetic modelling studies.

This study investigated the potential of employing plantain leaves as a natural bio-based sorbent for crude oil spill polluted seawater remediation. Type L9(34) Taguchi orthogonal array technique was used to evaluate the effect of four independent bio-sorption factors at three different levels (crude oil initial concentration (X1 7.8, 11.5 and 15.6 g/L), seawater-crude oil temperature (X2 25, 35 and 45 °C), bio-sorbent dosage (X3 1, 2 and 3 g) and bio-sorbent particle size (X4 1.18, 2.36 and 4.72 mm) on two response indices (bio-sorption efficiency (%) and bio-sorption capacity (g/g)). Taguchi optimization technique, numerical-desirability index function optimization technique and a proposed optimization method were utilized to determine the optimum bio-sorption factors needed for the optimum bio-sorption efficiency and bio-sorption capacity. The results demonstrated that the crude oil bio-sorption efficiency of the plantain leaves was significantly influenced by X1, X3 and X4 and the bio-sorption capacity was mainly influenced by X1 and X3. The optimum bio-sorption efficiency and the optimum bio-sorption capacity were 99.05 % and 12.82 g/g, respectively, obtained at optimum combination of factors and levels of X11 (7.8 g/L), X33 (3 g) and X41 (1.18 mm) for bio-sorption efficiency and X13 (15.6 g/L) X31 (1 g) for bio-sorption capacity. The Freundlich and Dubinin-Rudeshkevich isotherm models best explain the equilibrium bio-sorption data, while the pseudo-second order kinetic model best describes the bio-sorption kinetics. The bio-sorptive remediation mechanism followed dual mechanism of physical and chemical bio-sorption and the mass transfer controlled by film diffusion. The maximum bio-sorption capacity (Kf) was 14.0 gg-1.

Co-Influence of Nanofiller Content and 3D Printing Parameters on Mechanical Properties of Thermoplastic Polyurethane (TPU)/Halloysite Nanotube (HNT) Nanocomposites.

Thermoplastic polyurethane (TPU) belongs to a polyurethane family that possesses an elongation much higher than 300%, despite having low mechanical strength, which can be overcome by incorporating clay-based halloysite nanotubes (HNTs) as additives to manufacture TPU/HNT nanocomposites. This paper focuses on the co-influence of HNT content and 3D printing parameters on the mechanical properties of 3D printed TPU/HNT nanocomposites in terms of tensile properties, hardness, and abrasion resistance via fused deposition modelling (FDM). The optimum factor-level combination for different responses was determined with the aid of robust statistical Taguchi design of experiments (DoEs). Material characterisation was also carried out to evaluate the surface morphology, nanofiller dispersion, chemical structure, thermal stability, and phase behaviour corresponding to the DoE results obtained. It is evidently shown that HNT level and infill density play a significant role in impacting mechanical properties of 3D-printed TPU/HNT nanocomposites.

Microwave drying of quince coated with seed gum and pectin: A Taguchi optimization, techno-functional properties, and aromatic compounds.

In this study, the effects of coating quince slices with CaCl2 and pectin (C + P) followed by drying with microwave (MWD-C + P) or with hot air (HAD-C + P) were investigated to determine the physicochemical, techno-functional, textural, and volatile components of dried quince slices. A Taguchi orthogonal experimental design was set up with 18 points (L18 ), and the best conditions for drying were obtained using signal/noise ratio method. Coating quince slices with C + P and then drying with microwave at 450 W displayed the higher results compared to other points in terms of color, total phenolic, antioxidant activity, antimicrobial activity, and water holding capacity. MWD-C + P application dramatically changed the textural properties of dried quince slices in terms of hardness, gumminess, and chewiness. Moreover, MWD, lasted 12-15 min, was superior to HAD in the context of drying time. Ultrasonication as a pretreatment had no positive impact on dried products. GC-MS analyses revealed that MWD-C + P had positive effects on dried quince slices in terms of ethyl hexanoate and octanoic acid. However, MWD-C + P application triggered the formation of furfural in dried products.

High performance filtration membranes from electrospun poly (3-hydroxybutyrate)-based fiber membranes for fine particulate protection.

PM2.5 (particulate matter with a size of <2.5 µm) pollution has become a critical issue owing to its adverse health effects, including bronchitis, pneumonopathy, and cardiovascular diseases. Globally, around 8.9 million premature casualties related to exposure to PM2.5 were reported. Face masks are the only option that may restrict exposure to PM2.5. In this study, a PM2.5 dust filter was developed via the electrospinning technique using the poly (3-hydroxybutyrate) (PHB) biopolymer. Smooth and continuous fibers without beads were formed. The PHB membrane was further characterized, and the effects of the polymer solution concentration, applied voltage, and needle-to-collector distance were analyzed via the design of experiments technique, with three factors and three levels. The concentration of the polymer solution had the most significant effect on the fiber size and the porosity. The fiber diameter increased with increasing concentration, but decreases the porosity. The sample with a fiber diameter of ∼600 nm exhibited a higher PM2.5 filtration efficiency than the samples with a diameter of 900 nm, according to an ASTM F2299-based test. The PHB fiber mats fabricated at a concentration of 10%w/v, applied voltage of 15 kV, and needle tip-to-collector distance of 20 cm exhibited a high filtration efficiency of 95% and a pressure drop of <5 mmH2O/cm2. The tensile strength of the developed membranes ranged from 2.4 to 5.01 MPa, higher than those of the mask filters available in the market. Therefore, the prepared electrospun PHB fiber mats have great potential for the manufacture of PM2.5 filtration membranes.

Mechanical strength predictability of full factorial, Taguchi, and Box Behnken designs: Optimization of thermal settings and Cellulose Nanofibers content in PA12 for MEX AM.

Optimization of reinforced nanocomposites for MEX 3D-printing remain strong industrial claims. Herein, the efficacy of three modeling methods, i.e., full factorial (FFD), Taguchi (TD), and Box-Behnken (BBD), on the performance of MEX 3D printed nanocomposites was investigated, aiming to reduce the experimental effort. Filaments of medical-grade Polyamide 12 (PA12) reinforced with Cellulose NanoFibers (CNF) were evolved. Besides the CNF loading, 3D printing settings such as Nozzle (NT) and Bed (BΤ) Temperatures were optimization goals aiming to maximize the mechanical response. Three parameters and three levels of FFD were compliant with the ASTM-D638 standard (27 runs, five repetitions). An L9 orthogonal TD and a 15 runs BBD were compiled. In FFD, wt.3%CNF, 270 °C NT, and 80 °C BΤ led to 24% higher tensile strength compared to pure PA12. TGA, RAMAN, and SEM analyses interpreted the reinforcement mechanisms. TD and BBD exhibited fair approximations, requiring 7.4% and 11.8% of the FFD experimental effort.