RESUMEN
En el presente trabajo se registra la infección natural por Fasciola hepatica en un venado de cola blanca (Odocoileus virginianus) y en una taruca (Hippocamelus antisensis), ambos procedentes del departamento de Cusco. Los animales fueron remitidos al Instituto Veterinario (IVITA-Maranganí, FMV, UNMSM) por las autoridades del Servicio Nacional de Flora y Fauna (SERFOR, Sede Cusco). Durante la necropsia de los animales se colectaron seis trematodos de los conductos biliares, los cuales fueron preservados en etanol al 70%. Las observaciones morfológicas indicaron que se trataban de F. hepatica. Esto fue confirmado analizando el ADN mitocondrial de los parásitos amplificando parcialmente los genes citocromo c oxidasa subunidad 1 (cox1) y el NADH deshidrogenasa subunidad 1 (nad1). El análisis de estos genes tuvo una identidad mayor al 99% comparado con registros del banco de genes (GenBank). El presente estudio demuestra la presencia de F. hepatica en estos cérvidos, agregando así dos nuevos hospederos definitivos para el parásito.
Natural infection by Fasciola hepatica is recorded in a white-tailed deer (Odocoileus virginianus) and a taruca (Hippocamelus antisensis), both from the department of Cusco. Animals were remitted to the Veterinary Institute (IVITA-Maranganí, FMV, UNMSM) by the authorities of the National Service of Flora and Fauna (SERFOR, Cusco Headquarters). Six trematodes were collected from the bile ducts during the necropsy of the animals, and they were preserved in 70% ethanol. Morphological analysis indicated that they correspond to F. hepatica. This was confirmed by analyzing of the mitochondrial DNA of the parasites by partially amplifying the cytochrome c oxidase subunit 1 (cox1) and the NADH dehydrogenase subunit 1 (nad1) genes. Analysis of these genes had an identity greater than 99% compared to genes from GenBank. The present study demonstrates the occurrence of F. hepatica in these cervids, thus adding two new definitive hosts for the parasite.
RESUMEN
En el presente estudio se da a conocer tres especies de helmintos parásitos colectados de una taruca (Hippocamelus antisensis) en el Perú. Una taruca macho adulto fue remitido al Instituto Veterinario de Investigaciones Tropicales y de Altura (IVITA), Sede Maranganí, por el Servicio Nacional Forestal y de Fauna Silvestre del Perú (SERFOR), para su respectiva necropsia. Se colectaron 2 nematodos del sistema digestivo, así como un quiste localizado en el omento. Para el diagnóstico parasitológico se evaluaron las características morfo-métricas de los especímenes colectados. Los nematodos fueron diagnosticados como Trichostrongylus axei y Mazamastrongylus sp.; por otro lado, el quiste fue diagnosticado como un metacestodo de Taenia hydatigena. El hallazgo de T. axei representa el primer registro para la taruca. En la presente nota se realiza también una breve descripción morfológica de los helmintos mencionados.
This study presents three species of helminth parasites collected from a taruca (Hippocamelus antisensis) in Peru. Three helminth parasites from the taruca (Hippocamelus antisensis) are described in this report. An adult male taruca was remitted to the Veterinary Institute of Tropical and Highland Research (IVITA), Sede Maranganí, for the National Forest and Wildlife Service of Peru (SERFOR) for their respective necropsy. Two nematodes were collected from the digestive system, and 1 cyst was collected from the omentum. Morphometric characteristics of the specimens were evaluated for the parasitological diagnosis. Nematodes were diagnosed as Trichostrongylus axei and Mazamastrongylus sp.; on the other hand, the cyst was diagnosed as a metacestode of Taenia hydatigena. T. axei finding represents the first record for the taruca. A brief morphological description of each helminth and discussion about their hosts and distribution is done.
RESUMEN
BACKGROUND: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species. METHODS: Samples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a Hippocamelus antisense (blood and serum) were studied for the presence of A. marginale DNA through msp5 gene fragment amplification. Further characterization was done through MSP1a tandem repeats analysis and MLST scheme and the genetic relationship among previously characterized A. marginale sequences were studied by applying, eBURST algorithm and AMOVA analysis. RESULTS: Anaplasma marginale DNA was identified in the Myrmecophaga tridactyla and Hippocamelus antisense samples. Through molecular markers, we identified an identical genotype in both animals that was not previously reported in bovine host. The analysis through eBURST and AMOVA revealed no differentiation between the taruca/anteater isolate and the bovine group. CONCLUSIONS: In the present publication we report the identification of A. marginale DNA in a novel ruminant (Hippocamelus antisense) and non-ruminant (Myrmecophaga tridactyla) host species. Genotyping analysis of isolates demonstrated the close relatedness of the new isolate with the circulation population of A. marginale in livestock. Further analysis is needed to understand whether these two hosts contribute to the anaplasmosis epidemiology.
Asunto(s)
Anaplasma marginale/clasificación , Anaplasmosis/microbiología , Artiodáctilos/microbiología , Enfermedades de los Bovinos/microbiología , Xenarthra/microbiología , Anaplasma marginale/aislamiento & purificación , Animales , Proteínas de la Membrana Bacteriana Externa/genética , Bovinos , ADN Bacteriano/sangre , Femenino , Genotipo , Especificidad del Huésped , Tipificación de Secuencias Multilocus/veterinaria , Filogenia , Análisis de Secuencia de ADN/veterinaria , Secuencias Repetidas en Tándem/genéticaRESUMEN
In the present report metacestodes were collected from the mesentery of a taruca (Hippocamelus antisensis) and from the omentum of a red brocket deer (Mazama americana) in Peru. Various metacestodes parameters, including rostellar hook characteristics, were measured. Molecular analysis was performed to amplify the mitochondrial cytochrome c oxidase subunit 1 gene from metacestode isolates. Metacestodes were identified as T. hydatigena by morphology and molecular methods. This constitutes the first molecular detection of T. hydatigena metacestodes in the taruca and the red brocket deer and demonstrates that these animal species are natural intermediate hosts for this parasite.
Asunto(s)
Cisticercosis/veterinaria , Ciervos , Taenia/clasificación , Animales , Animales de Zoológico , Cisticercosis/epidemiología , Cisticercosis/parasitología , Masculino , Perú/epidemiología , Filogenia , Taenia/genéticaRESUMEN
Con la finalidad de evaluar la influencia de la adición de DL- Metionina en la alimentación de cuyes y el comportamiento productivo de las raciones balanceadas de alimentos en el crecimiento en de cuyes (Cavia porcellus), para lo cual se emplearon 32 roedores entre machos y hembras mejorados, el crecimiento fue evaluado hasta las seis semanas de edad siendo su alimentación con concentrados y la adición de DL-Metionina con el 0,22 por ciento, maschala y alfalfa, con 16 por ciento de proteína, con dos raciones: una con metionina otra sin ella. A las seis semanas los cuyes alcanzaron pesos finales de 756,25 g. para hembras y de 780 g. para los del género opuesto, los resultados muestran diferencias estadísticas no muy relevantes, con los resultados obtenidos en el crecimiento se determinaron los costos y se calcuó la rentabilidad. El valor comercial de loscuyes es de S/. 10,00 nuevos soles, el peso final determina el incremento de peso de 377,5 g. se alcanza la mejor relación Costo/Beneficio de 23,8 por ciento, la mayor utilidad y la mejorrentabilidad cuando se utiliza metionina.
To evaluate the influence of the addition of DL - Metionina in guinea-pigs's nuthtion and the productive behavior of the balanced portions of food in the guinea pigs' nutrition (Cavia porcellus) for which it was used 32 rodents between males and females Improved, the growth was evaluated during the six first weeks with concéntrate food and the addition of DL. Metionina with 0,22 and chalk and alfalfa, with 16 per cent of protein, with two portions: one with metionina and other without it. Past the six weeks the guinea pigs had weights of 756,25 g, in females and 780 g. in males, the aftermaths show statistical differences not very relevant, with the results in the growth of guinea pigs the cost was determined and profitability was calculated. The Guinea-pigs's commercial value is SI. 10,00 nuevos soles, the final weight determines the weight's increase to 377,5 g. andit's caught the best relation cost/ profit with 23,8 per cent the best profit and the best profitability when metionina is used.
