pH-responsive resveratrol-loaded ZIF-8 nanoparticles modified with tannic acid for promoting colon cancer cell apoptosis.

Resveratrol (Res) is known for its potential in treating various types of cancers, with a particular advantage of causing minimal toxic side effects. However, its clinical application is constrained by challenges such as poor bioavailability, low water solubility, and chemical instability in neutral and alkaline environments. In light of these limitations, we have developed a pH-responsive drug delivery nanoplatform, Res@ZIF-8/TA NPs, which exhibits good biocompatibility and shows promise for in vitro cancer therapy. Benefiting from the mild reaction conditions provided by zeolitic imidazolate frameworks (ZIFs), a "one-pot method" was used for drug synthesis and loading, resulting in a satisfactory loading capacity. Notably, Res@ZIF-8/TA NPs respond to acidic environments, leading to an improved drug release profile with a controlled release effect. Our cell-based experiments indicated that tannic acid (TA) modification enhances the biocompatibility of ZIFs. 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (MTT assay), Hoechst 33342/PI staining, cell scratch assay, Transwell and Reverse Transcription quantitative PCR (RT-qPCR) assays further demonstrated that Res@ZIF-8/TA NPs inhibited colon cancer cell migration and invasion, and promoted apoptosis of colon cancer cells, suggesting a therapeutic potential and demonstrating anti-cancer properties. In conclusion, the Res@ZIF-8/TA NPs pH-responsive drug delivery systems we developed may offer a promising avenue for cancer therapy. By addressing some of the challenges associated with Res-based treatments, this system could contribute to advancements in cancer therapeutics.

Transport of ZIF-8 in porous media under the influence of surfactant type and nanoparticle concentration.

Knowledge of the fate and transport of metal-organic frameworks (MOFs) in porous media is essential to understanding their environmental impacts. However, to date, the transport mechanisms of MOFs are not fully revealed. Meanwhile, surfactants can promote MOFs dispersion by forming a stable suspension. They also allow MOFs to migrate in the aqueous environment, which would increase the risks of MOFs being exposed to human health and the ecological environment. In this study, the effect of surfactants type and nanoparticle (NP) concentrations (50, 100, and 200 mg/L) were investigated using a sand column to study the transportability of ZIF-8 NPs in saturated porous media. Surfactants used were categorized into three groups, including cationic surfactants (CTAB, DTAB), anionic surfactants (SDBS, SDS), and nonionic surfactants (Tween 80, Tween 20). Experimental results showed that the ionic surfactants significantly increased the transportability of ZIF-8 NPs. Furthermore, a low concentration of NPs tended to break through the column under ionic surfactant conditions, and the maximum effluent recovery of ZIF-8 NPs (50 mg/L) was 87.4% in the presence of SDS. Nevertheless, ZIF-8 NPs tended to deposit in the inlet of the sand column in the presence of nonionic surfactants due to hydrodynamic bridging and straining. This research provides a comprehensive understanding of the deposition mechanism of ZIF-8 NPs as affected by surfactant types and NP concentrations. Most importantly, the study highlights those ionic surfactants had a significant impact on the mobility of ZIF-8 NPs, which arouses attention to the ecological and human health risk assessment related to the manufacturing of MOFs with the aid of various dispersing agents.

ZIF-8 Nanoparticles Based Electrochemical Sensor for Non-Enzymatic Creatinine Detection.

There is a consistent demand for developing highly sensitive, stable, cost-effective, and easy-to-fabricate creatinine sensors as creatinine is a reliable indicator of kidney and muscle-related disorders. Herein, we reported a highly sensitive and selective non-enzymatic electrochemical creatinine sensor via modifying poly(3,4 ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) coated indium tin oxide (ITO) substrate by zeolitic imidazolate framework-8 nanoparticles (ZIF-8 NPs). The topography, crystallinity, and composition of the sensing electrode were characterized by field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The peroxidase-like activity of ZIF-8 nanoparticles enabled it to detect creatinine forming a zinc-creatinine composite. The electrochemical behavior and sensing performance were evaluated by amperometric and impedimetric analysis. The sensor obtained a sufficiently low limit of detection (LOD) of 30 µM in a clinically acceptable linear range (0.05 mM-2.5 mM). The interference study demonstrated high selectivity of the sensor for creatinine concerning other similar biomolecules. The sensing performance of the creatinine sensor was verified in the actual human serum, which showed excellent recovery rates. Hence, the magnificent performance of ZIF-8 based non-enzymatic creatinine sensor validated it as a responsible entity for other complicated renal markers detection.