Palearctic flea beetle and pest of hops and Cannabis, Psylliodesattenuata (Coleoptera, Chrysomelidae, Galerucinae), new to North America.

Background: The univoltine leaf beetle Psylliodesattenuata (Koch, 1803) is a pest of Cannabis and Humulus (Cannabaceae) and native to the Palaearctic Region, known from eastern Asia to western Europe. New information: First North American records are presented for P.attenuata from Canada: Ontario and Québec. Adult beetle feeding damage to hops Humuluslupulus L. (Cannabacaea) plants is recorded from Québec. Diagnostic information is presented to distinguish P.attenuata from other North American Chrysomelidae and a preliminary assessment of its potential to spread in North America is presented. While our climate analysis is limited by a lack of data, it appears P.attenuata is physiologically capable of persisting throughout the range of Humulus in North America.The United States of America and Canada are now known to be home to 71 or more species of adventive Chrysomelidae.

Inventory of the urban flora of Budapest (Hungary) highlighting new and noteworthy floristic records.

Background: The systematic urban floristic research of Budapest was started in 2018 by the authors with detailed methodology. One scope of the research was to gain knowledge on the plant taxa appearing in Budapest and to compile the inventory of the urban flora of Budapest. New information: We have provided the inventory of the urban flora of Budapest, which includes distribution data for all 973 taxa found in Budapest between May 2018 and May 2023. We also provided new detailed occurrence data for 49 species in Budapest. Seven of them are new to the adventive flora of Hungary (Campanulaportenschlagiana Roem. & Schult., Clinopodiumnepeta (L.) Kuntze, Chasmanthiumlatifolium (Michx.) H.O.Yates, Cyrtomiumfortunei J.Sm., Linariamaroccana Hook.f., Talinumpaniculatum (Jacq.) Gaertn.), three were rediscovered in Hungary (Glebioniscoronaria (L.) Cass. ex Spach, Lagenariasiceraria (Molina) Standl., Sisymbriumirio L.) and 18 were recorded for the first time in Budapest. We also provided data for two data-poor (Artemisiascoparia Waldst. & Kit., Polygonumrurivagum Jord. ex Boreau) species and we documented the major expansion of six species.

Field releases of the exotic parasitoid Trissolcus japonicus (Hymenoptera: Scelionidae) and survey of native parasitoids attacking Halyomorpha halys (Hemiptera: Pentatomidae) in Michigan.

An adventive population of the exotic parasitoid wasp, Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae), discovered in Michigan in 2018, is a promising biological control agent of the invasive Halyomorpha halys (Stål) (Hemiptera: Pentatomidae). Following its discovery, field releases of Tr. japonicus were conducted over 2 yr in southern Michigan, to test how release size or release frequency impacts establishment. Sentinel eggs of H. halys and of the native Podisus maculiventris (Say) (Hemiptera: Pentatomidae) were used alongside yellow sticky cards to monitor parasitoids. In 2019 and 2020, 7,200 Tr. japonicus were released at 16 sites. Monitoring between 2019 and 2021 yielded only 49 individuals. The captures suggest reproductive activity and overwintering success in the field but do not allow for evaluation of best release methods. Parasitism by native parasitoids was below 7%, which is similar to other states and unlikely to provide sufficient control of H. halys. The placement of sentinel eggs or sticky traps either in the lower or middle canopy of trees did not influence parasitoid capture rates. Frozen and fresh H. halys sentinel eggs were attacked at the same rate, but more native parasitoids emerged from frozen eggs. We did not find signs of nontarget effects on P. maculiventris thus parasitism rates overall were very low. These results could indicate dispersal of Tr. japonicus from the release sites or slow population growth. The latter may be due to the relatively low densities of H. halys in Michigan or may stem from the small founding size of our laboratory colony.

Palaearctic leaf beetle Chrysolinafastuosa (Coleoptera, Chrysomelidae, Chrysomelinae) new to North America.

Background: The univoltine leaf beetle Chrysolinafastuosa (Scopoli, 1763) is native to in the Palearctic Region from eastern Siberia to western Europe. New information: First North American records are presented for C.fastuosa (Scopoli, 1763) (Coleoptera, Chrysomelidae, Chrysomelinae), as confirmed by vouchered specimens from Canada: Nova Scotia. Additional citizen science records from USA: Vermont are also discussed. Diagnostic information is presented to distinguish C.fastuosa from other North American Chrysomelidae and a species distribution model to assess its potential spread in North America is presented. This insect is expected to cause some feeding damage to above-ground parts of ornamental and invasive Lamiaceae, especially species of Galeopsis L. The species distribution model and the range of its host plant Galeopsistetrahit, suggest the north-eastern US and south-eastern Canada, from the Atlantic coast to the west end of Lake Superior provide the most suitable conditions for this species. The United States of America and Canada are now known to be home to 70 or more species of adventive Chrysomelidae.

The worldwide spread of Aedes albopictus: New insights from mitogenomes.

The tiger mosquito (Aedes albopictus) is one of the most invasive species in the world and a competent vector for numerous arboviruses, thus the study and monitoring of its fast worldwide spread is crucial for global public health. The small extra-nuclear and maternally-inherited mitochondrial DNA represents a key tool for reconstructing phylogenetic and phylogeographic relationships within a species, especially when analyzed at the mitogenome level. Here the mitogenome variation of 76 tiger mosquitoes, 37 of which new and collected from both wild adventive populations and laboratory strains, was investigated. This analysis significantly improved the global mtDNA phylogeny of Ae. albopictus, uncovering new branches and sub-branches within haplogroup A1, the one involved in its recent worldwide spread. Our phylogeographic approach shows that the current distribution of tiger mosquito mitogenome variation has been strongly affected by clonal and sub-clonal founder events, sometimes involving wide geographic areas, even across continents, thus shedding light on the Asian sources of worldwide adventive populations. In particular, different starting points for the two major clades within A1 are suggested, with A1a spreading mainly along temperate areas from Japanese and Chinese sources, and A1b arising and mainly diffusing in tropical areas from a South Asian source.

Adventive Larval Parasitoids Reconstruct Their Close Association with Spotted-Wing Drosophila in the Invaded North American Range.

Two species of larval parasitoids of the globally invasive fruit pest, Drosophila suzukii (Diptera: Drosophilidae), Leptopilina japonica, and Ganaspis brasiliensis (both Hymenoptera: Figitidae), were detected in British Columbia, Canada in 2016 and 2019, respectively. Both are presumed to have been unintentionally introduced from Asia; however, the extent of their establishment across different habitats with diverse host plants used by D. suzukii was unclear. In addition, there was no knowledge of the temporal dynamics of parasitism of D. suzukii by these two parasitoids. To address these gaps, we repeatedly sampled the fruits of known host plants of D. suzukii over the entire 2020 growing season in British Columbia. We documented the presence of L. japonica and G. brasiliensis and estimated the apparent percentage of D. suzukii parasitized among host plant species. Across a large region of southwestern British Columbia, both L. japonica and G. brasiliensis were found to be very common across a variety of mostly unmanaged habitats over the entire course of the season (May-October) in the fruits of most host plants known to host D. suzukii larvae. Parasitism of D. suzukii was variable (0-66% percent parasitism) and appeared to be time-structured. Our study demonstrates that the close association between the two larval parasitoids and D. suzukii that exists in Asia has evidently been reconstructed in North America, resulting in the highest parasitism levels of D. suzukii yet recorded outside of its area of origin.

Ten-year trends reveal declining quality of seeded pollinator habitat on reclaimed mines regardless of seed mix diversity.

Plant-pollinator interactions represent a crucial ecosystem function threatened by anthropogenic landscape changes. Disturbances that reduce plant diversity are associated with floral resource and pollinator declines. Establishing wildflower plantings is a major conservation strategy targeting pollinators, the success of which depends on long-term persistence of seeded floral communities. However, most pollinator-oriented seeding projects are monitored for a few years, making it difficult to evaluate the longevity of such interventions. Selecting plant species to provide pollinators diverse arrays of floral resources throughout their activity season is often limited by budgetary constraints and other conservation priorities. To evaluate the long-term persistence of prairie vegetation seeded to support pollinators, we sowed wildflower seed mixes into plots on a degraded reclaimed strip-mine landscape in central Ohio, USA. We examined how pollinator habitat quality, measured as floral abundance and diversity, changed over 10 years (2009-2019) in the absence of management, over the course of the blooming season within each year, and across three seed mixes containing different numbers and combinations of flowering plant species. Seeded species floral abundance declined by more than 75% over the study, with the largest decline occurring between the fifth and seventh summers. Native and non-native adventive flowering plants quickly colonized the plots and represented >50% of floral community abundances on average. Floral richness remained relatively constant throughout the study, with a small peak one year after plot establishment. Plots seeded with High-Diversity Mixes averaged two or three more species per plot compared with a Low-Diversity Mix, despite having been seeded with twice as many plant species. Within years, the abundance and diversity of seeded species were lowest early in the blooming season and increased monotonically from June to August. Adventive species exhibited the opposite trend, such that complementary abundance patterns of seeded and adventive species blooms resulted in a relatively constant floral abundance across the growing season. Seeded plant communities followed classic successional patterns in which annual species quickly established and flowered but were replaced by perennial species after the first few summers. Long-term data on establishment and persistence of flower species can guide species selection for future-oriented pollinator habitat restorations.

New records of Curculionoidea from Newfoundland and Labrador, with the first records of Orthochaetessetiger ([Beck]) (Curculionidae, Curculioninae, Styphlini) for North America.

Thirty species of Curculionoidea (28 Curculionidae and one each of Brentidae and Nemonychidae) are reported as new records from the Canadian province of Newfoundland and Labrador, most of them from the island of Newfoundland. As well, 13 species of Curculionidae and one of Brentidae previously recorded from Newfoundland are newly reported from Labrador, and one Curculionidae previously recorded from Labrador is newly reported from Newfoundland. The Palearctic species, Orthochaetessetiger ([Beck]), is herein reported as a new Canadian and North American record, with specimens documented from Newfoundland and British Columbia. Additions to the primary key for North American weevils are provided to help identify this genus among the North American fauna. Of the species of Curculionoidea previously recorded from the province in published literature, there is uncertain evidence for the occurrence of 14 species in the province as a whole or in the Labrador portion. Seven species are hereby removed from the faunal list for the province. One of those, Trachodeshispidus (Linnaeus), is also removed from the Canadian faunal list. The 134 species of Curculionoidea recorded from NL are listed and a brief synopsis of the fauna provided.

A Review of Dung Beetle Introductions in the Antipodes and North America: Status, Opportunities, and Challenges.

Following the introduction of cattle, exotic dung beetles (Coleoptera: Aphodiidae, Geotrupidae, Scarabaeidae) were imported into the Antipodes (Australia and New Zealand) and North America (primarily the United States) to accelerate the degradation of cattle dung on pastures. The history of dung beetle introductions between the two regions is similar but has not previously been assessed: this is important as new introductions are continuing in the regions. Here, we review these introduction programs, report on their current status, and discuss methodological advances. In doing so, we examine the accidental introduction of exotic (i.e., adventive) species and the contribution of both deliberately introduced and adventive species to endemic dung beetle faunas. Further, we provide a list of pest and parasite species whose populations can be reduced by dung beetle activity. We also identify a combined total of 37 introduced and 47 adventive dung beetle species that have become established in the Antipodes and North America, with exotic species dominating dung beetle assemblages from pasture habitats. Climatic and edaphic matches, the size of founding populations, abiotic and biotic stressors, and the time of year when releases are made are all critical determinants that affect the success of dung beetle introduction programs. Finally, we discuss opportunities, plus the risks and challenges associated with dung beetle introductions. We hope that this review will aid in the success of future introduction programs, either to enhance ecosystem services in areas that they are needed, or potentially to reestablish native species in regions where they have been extirpated.

Establishment of a non-native xyleborine ambrosia beetle, Xyleborus monographus (Fabricius) (Coleoptera: Curculionidae: Scolytinae), new to North America in California.

Specimens of an ambrosia beetle, Xyleborus monographus (Fabricius), were found infesting oak trees in California. This is the first record of this species established in North America. Based on collection information, this species most likely has been established in the Napa County area for several years. A modified key to Xyleborus in North America, and diagnosis of the species is provided.

The bark and ambrosia beetles (Coleoptera: Curculionidae: Scolytinae and Platypodinae) of American Samoa.

A survey of five of the islands of American Samoa was conducted from 2016-2018 utilizing multi-funnel traps baited with ethanol and quercivorol (attractants for xyleborine ambrosia beetles). Specimens of Scolytinae and Platypodinae from this survey, as well as specimens in the American Samoa Community College Collection were identified. A total of 53 species of Scolytinae and two species of Platypodinae are reported. Fourteen species of Scolytinae and one species of Platypodinae are reported as new to American Samoa. The following new synonymy and new combinations are proposed: Xyleborinus artelineatus (Beeson, 1929) (=Xyleborinus cinctipennis (Schedl, 1980) syn. nov.; Ambrosiophilus wilderi (Beeson, 1929) comb. nov. from Ambrosiodmus; Beaverium swezeyi (Beeson, 1929) comb. nov. from Xyleborus; and Debus fallaxoides (Schedl, 1955) comb. nov. from Xyleborus. The distribution on the islands of American Samoa and taxonomic history of each species is provided.

DNA barcodes reveal 63 overlooked species of Canadian beetles (Insecta, Coleoptera).

This study demonstrates the power of DNA barcoding to detect overlooked and newly arrived taxa. Sixty-three species of Coleoptera representing 25 families are studied based on DNA barcode data and morphological analysis of the barcoded specimens. Three of the species involve synonymies or previous taxonomic confusion in North America, while the first Canadian records are published for 60 species. Forty-two species are adventive in North America, and 40 of these adventive species originate from the Palaearctic region. Three genera are recorded from the Nearctic region for the first time: Coelostoma Brullé, 1835 (Hydrophilidae), Scydmoraphes Reitter, 1891 (Staphylinidae), and Lythraria Bedel, 1897 (Chrysomelidae). Two new synonymies are established: Mycetoporus triangulatus Campbell, 1991 (Staphylinidae) is a junior synonym of Mycetoporus reichei Pandellé, 1869, syn. nov. while Bledius philadelphicus Fall, 1919 (Staphylinidae) is a junior synonym of Bledius gallicus (Gravenhorst, 1806), syn. nov. The previously suggested move of Ctenicera tigrina (Fall, 1901) to the genus Pseudanostirus Dolin, 1964 (Elateridae) is formalized, resulting in Pseudanostirus tigrinus (Fall, 1901), comb. nov.

Occurrence and distribution of Ambylomma americanum as determined by passive surveillance in Ontario, Canada (1999-2016).

The lone star tick, Amblyomma americanum, is spreading northward from its historical stronghold in the southeastern United States. As a vector and biting pest, public and veterinary health officials must remain vigilant of the lone star tick's expanding range. We use ticks submitted to Public Health Ontario Laboratory (1999-2016) to describe the spatial and temporal dynamics of A. americanum in Ontario, Canada, as well as submitter demographics. We identified 847 A. americanum submissions during the surveillance period, with 773 (91.3%) non-travel-related and 74 (8.7%) travel-related submissions. Annual A. americanum submissions increased over the surveillance period. Approximately 91% of non-travel-related submissions were adult ticks and 9% were nymphs. The highest submission rates were from individuals living in the Eastern and South West regions of the province. Adult specimens were primarily submitted from May through July and nymphs from March through September. Higher numbers of submissions were from young children (<10 years) and older adults (55-74 years), with equal proportions of male and female submitters. The majority of travel-related submissions were from travellers returning from the southeastern United States (i.e., Florida, North Carolina, South Carolina, Tennessee, Texas). Amblyomma americanum distribution is scattered in Ontario and submissions are likely the consequence of ongoing detection of adventive specimens. Further tick dragging is required to confirm the presence of established lone star tick populations in the province. Given the relatively rapid expansion of blacklegged ticks, Ixodes scapularis, populations in Ontario, we expect climate change to facilitate the range of expansion of A. americanum into the province. We propose an algorithm for identifying A. americanum-risk areas, which will aid public and veterinary health officials when assessing the risks posed by lone star ticks.

First records of Cyclorhipidion fukiense (Eggers) (Coleoptera: Curculionidae: Scolytinae: Xyleborini), an ambrosia beetle native to Asia, in North America.

The Asian ambrosia beetle, Cyclorhipidion fukiense (Eggers) was detected for the first time in North America based on three specimens trapped in 2012 from three localities in South Carolina and two other specimens intercepted at the port of Savannah, Georgia, in 2010. The species is characterized, illustrated with high-resolution images, and compared with two other congeneric, adventive species (C. bodoanum and C. pelliculosum) presently established in eastern North America. Morphometric measurements are provided and a provisional key is presented to the species of Cyclorhipidion occurring in North America.

Checklist of bees (Apoidea) from a private conservation property in west-central Montana.

BACKGROUND: Here we present preliminary results from the first three years of a long-term bee survey conducted at a 3,840-ha private conservation property in the northern Sapphire Mountains and Bitterroot River Valley, and a pilot study at an associated 80-ha property in the Swan River Valley, Missoula County, Montana, USA. The survey includes hand-net, bowl-trap, and blue-vane trap collections. The resulting checklist comprises 229 bee species and morphospecies within 5 families, 38 genera and 91 subgenera. Of the total species in the list, 34 of them represent first state records Montana. This survey expands the number of bee species recorded in Montana to 366. Included in these species is Megachile (Eutricharaea) apicalis Spinola, showing a range expansion for this introduced bee. NEW INFORMATION: We present new distributional records for 34 bee species, including Megachile (Eutricharaea) apicalis Spinola, an introduced bee that was discovered to be resident in North America in 1984 in Santa Barbara County, California. This species has since expanded its range in the across the west, but had not been previously recorded in Montana.

The Giant Resin Bee, Megachile sculpturalis Smith: New Distributional Records for the Mid- and Gulf-south USA.

BACKGROUND: Megachile (Callomegachile) sculpturalis Smith, the giant resin bee, is an adventive species in the United States. First established in the United States during the early 1990s, records currently exist from most states east of the Mississippi River along with Iowa and Kansas. NEW INFORMATION: New distributional records are presented for Megachile (Callomegachile) sculpturalis Smith, an introduced bee. Additional records presented here expand the known distribution southwest through Arkansas, Louisiana, Mississippi, Missouri, and Texas. An updated host plant list containing new records is also presented, expanding the number of known floral associations.

Variation in Effects of Conophthorin on Catches of Ambrosia Beetles (Coleoptera: Curculionidae: Scolytinae) in Ethanol-Baited Traps in the United States.

In 2013, we examined the effects of conophthorin on flight responses of ambrosia beetles (Coleoptera: Curculionidae: Scolytinae) to multiple-funnel traps baited with ethanol in Georgia, Michigan, New Hampshire, and Oregon. Adventive species (=exotic, nonnative, immigrant, introduced) accounted for 91.4% of total catches of ambrosia beetles. Conophthorin increased catches of Xyleborinus saxesenii (Ratzeburg) in Georgia, New Hampshire, and Oregon. Catches of Cyclorhipidion pelliculosum (Eichhoff) were increased by conophthorin in New Hampshire but not in Michigan. In Oregon, conophthorin decreased catches of Xylosandrus germanus (Blandford) to ethanol-baited traps but not in Michigan and New Hampshire. In Georgia, conophthorin increased catches of Gnathotrichus materiarius (Fitch), Xyleborus spp., and Xylosandrus crassiusculus (Motschulsky) but decreased catches of Cnestus mutilatus (Blandford), Dryoxylon onoharaensum (Murayama), and Cyclorhipidion bodoanum (Reitter). Conophthorin had no effect on catches of Ambrosiophilus atratus (Eichhoff), Anisandrus dispar (F.), Anisandrus sayi (Hopkins), Gnathotrichus sulcatus (Leconte), Monarthrum fasciatum (Say), Monarthrum mali (Fitch), and Xylosandrus compactus (Eichhoff). Attraction of the bark beetle, Hypothenemus rotundicollis (Eichhoff), was interrupted by conophthorin in Georgia. Our results suggest that adding conophthorin lures to traps baited with ethanol may have utility in detection programs in North America and overseas. However, traps baited with ethanol alone should also be used due to interruption in attraction for some species of ambrosia beetles.

Ipsenol, Ipsdienol, Ethanol, and α-Pinene: Trap Lure Blend for Cerambycidae and Buprestidae (Coleoptera) in Pine Forests of Eastern North America.

In 2007-2008, we examined the flight responses of wood-boring beetles (Coleoptera: Cerambycidae and Buprestidae) to multiple-funnel traps baited with the pine volatiles, ethanol, and α-pinene [85% (-)], and the bark beetle pheromones, racemic ipsenol and racemic ipsdienol. Experiments were conducted in mature pine stands in Canada (Ontario and New Brunswick) and the United States (Arkansas, Florida, Michigan, New Hampshire, North Carolina, Ohio, Tennessee, and Wisconsin). At each location, traps were deployed in 10 replicate blocks of four traps per block. The trap treatments were: 1) blank control; 2) ipsenol and ipsdienol; 3) ethanol and α-pinene; and 4) a quaternary blend of ipsenol, ipsdienol, ethanol, and α-pinene. Traps baited with the quaternary blend caught the greatest numbers of Acanthocinus nodosus (F.), Acanthocinus obsoletus (Olivier), Acmaeops proteus (Kirby), Astylopsis sexguttata (Say), Rhagium inquisitor (L.) (Cerambycidae), and Buprestis lineata (F.) (Buprestidae). Traps baited with ethanol and α-pinene caught the greatest numbers of Arhopalus rusticus (LeConte), Asemum striatum (L.), Tetropium spp., Xylotrechus sagittatus (Germar) (Cerambycidae), and Buprestis maculipennis Gory (Buprestidae) with minimal interruption by ipsenol and ipsdienol. Our results suggest that multiple-funnel traps baited with the quaternary lure blend of ipsenol, ipsdienol, ethanol, and α-pinene are effective for trapping various species of wood-boring beetles in pine forests of eastern North America, and may have utility in detection programs for adventive species in North America and overseas.

Responses of Cerambycidae and Other Insects to Traps Baited With Ethanol, 2,3-Hexanediol, and 3,2-Hydroxyketone Lures in North-Central Georgia.

In north-central Georgia, 13 species of woodboring beetles (Coleoptera: Cerambycidae: Cerambycinae) were attracted to multiple-funnel traps baited with ethanol and one of the following pheromones: (1) racemic 3-hydroxyhexan-2-one; (2) racemic 3-hydroxyoctan-2-one; and (3) syn-2,3-hexanediol. The following species were attracted to traps baited with ethanol and 3-hydroxyhexan-2-one: Anelaphus pumilus (Newman), Eburia quadrigeminata (Say), Euderces pini (Olivier), Knulliana cincta (Drury), Neoclytus mucronatus (F.), Neoclytus scutellaris (Olivier), and Xylotrechus colonus (F.). Clytus marginicollis Castelnau & Gory, and Anelaphus parallelus (Newman) were attracted to traps baited with ethanol and 3-hydroxyoctan-2-one, whereas traps baited with ethanol and syn-2,3-hexanediol were attractive to Anelaphus villosus (F.), A. parallelus, Neoclytus acuminatus (F.), Neoclytus jouteli jouteli Davis, and Megacyllene caryae (Gahan). Ethanol enhanced catches of seven cerambycid species in traps baited with syn-2,3-hexanediol and 3,2-hydroxyketones. Catches of bark and ambrosia beetles (Curculionidae: Scolytinae) in ethanol-baited traps were largely unaffected by the addition of syn-2,3-hexanediol and 3,2-hydroxyketone lures, except for two species. The mean catches of Hypothenemus rotundicollis Wood & Bright and Dryoxylon onoharaensum (Murayama) in ethanol-baited traps increased and decreased, respectively, with the addition of racemic 3-hydroxyoctan-2-one. Traps baited with ethanol and syn-2,3-hexanediol were attractive to Xylobiops basilaris (Say) (Bostrichidae) and Chariessa pilosa (Forster) (Cleridae), whereas Temnoscheila virescens (F.) (Trogossitidae) were attracted to traps baited with ethanol and 3-hydroxyhexan-2-one. The assassin bug, Apiomerus crassipes (F.) (Hemiptera: Reduviidae), was attracted to traps baited with ethanol and 3,2-hydroxyketones.

A review of Canadian and Alaskan species of the genera Clusiota Casey and Atheta Thomson, subgenus Microdota Mulsant & Rey (Coleoptera, Staphylinidae, Aleocharinae).

This paper treats 13 species of the subgenus Microdota Mulsant & Rey of Atheta Thomson and 3 species of the genus Clusiota Casey in Canada and Alaska. We report here 4 species new to science, and 3 new provincial records. The following species are new to science: Atheta (Microdota) curtipenis Klimaszewski & Webster, sp. n., Atheta (Microdota) formicaensis Klimaszewski & Webster, sp. n., Atheta (Microdota) macesi Klimaszewski & Webster, sp. n., and Clusiota grandipenis Klimaszewski & Webster, sp. n. The new provincial records are: Atheta (Microdota) pseudosubtilis Klimaszewski & Langor, new to AB, and Atheta (Microdota) subtilis (Scriba), an adventive Palaearctic species new to North America, first reported in LB and NB. The two Clusiota Casey species are reviewed, and their distribution is revised. A female Clusiota impressicollis was discovered in Ontario and is illustrated here for the first time. A key to all Canadian species of the subgenus Microdota and genus Clusiota are provided. Atheta (Microdota) holmbergi Bernhauer and Atheta (Microdota) alesi Klimaszewski & Brunke are transferred here to the subgenus Dimetrota Mulsant & Rey.