Changes in the activity budget of the fiddler crab Leptuca uruguayensis throughout the reproductive period in temperate estuaries.

Animal reproductive success implies the performance of several behaviours, such as courting, mate searching, copulation, offspring production and care. These behaviours usually have high energetic and ecological costs. Therefore, to maximise their reproductive success, animals should make choices throughout their lives, such as deciding how much energy to invest in different activities, according to their conditions and needs. In temperate estuaries, the fiddler crab L. uruguayensis has a short reproductive period, with two synchronous spawning events. Considering that reproductive behaviours incur high energetic cost to fiddler crabs, we estimated how this species manages its activity budget throughout the reproductive period, to quantify trade-offs between the time spent on reproductive behaviours versus time spent on other activities. By analysing videos of females and males recorded in the field at different moments of the reproductive period, we observed that pre-copulatory behaviours, such as female wandering and male waving were more intense at the beginning of the reproductive period, suggesting that most matings occurred before the first spawning event but not before the second one. The ecological conditions during the breeding season and the individual strategies adopted by males and females mostly determine when and how much time to spend on courtship behaviours, and behavioural plasticity can be expected whenever the conditions change. The strategy used by L. uruguayensis for energy management, females' ability to store male gametes and environmental temperatures might have been the main factors determining the relative time spent in courtship behaviours during the reproductive period.

Cacao breeding in Colombia, past, present and future.

Cacao (Theobroma cacao L.) is considered a key crop in Colombian social programs aiming at alleviating rural poverty, promoting peace in post-conflict regions and, replacing crops used for illicit purposes. Colombia is thought to be part of the center of origin of cacao; several germplasm collecting expeditions have been implemented, dating back to the 1940s. Despite that history, the first breeding program based on creating, selecting, and releasing full-sib progenies made extensive use of accessions introduced from other countries as parents. A new breeding strategy was adopted in the 1990s, based on mass selection of promising trees (high-yield and disease-resistant) in farmers' fields, resulting in the selection of clones released to farmers as planting material. In 2012, a new strategy, Recurrent Selection, was adopted by the Colombian Corporation for Agricultural Research, Agrosavia, based on the development of improved populations and allowing the selection of clones at the end of each cycle of recombination. The use of molecular markers is being integrated into this program in order to assist breeders in selecting material. This review provides details about the history and perspectives of the cacao breeding program in Colombia.