The diversity, drivers, consequences and management of plant invasions in the mangrove ecosystems.

Mangrove ecosystems, which occupy intertidal environments across tropical and subtropical regions, provide crucial ecosystem services, such as protecting the coastal areas by reducing the impact of cyclones, storms, and tidal waves. Anthropogenic activities such as human settlements, deforestation, pollution, and climate change have increased the risk of biological invasions in mangrove habitats. Plant species can be introduced to mangrove habitats via anthropogenic means, such as trade and transportation, urbanisation, and agriculture, as well as through natural processes like wind, floods, cyclones, and animal-assisted seed dispersal. Additionally, some native plants can become invasive due to the changes in the mangrove ecosystem. Invasive species can significantly affect coastal ecosystems by out-competing native flora for resources, thereby altering fundamental properties, functions, and ecosystem services of the mangrove forests. The successful establishment of invasive species depends on a complex interplay of factors involving the biological attributes of the invading species and the ecological dynamics of the invaded habitat. This review focuses on exploring the mechanisms of invasion, strategies used by invasive plants, the effects of invasive plants on mangrove habitats and their possible management strategies. Based on the literature, managing invasive species is possible by biological, chemical, or physical methods. Some non-native mangrove species introduced through restoration activities can often become more intrusive than native species. Therefore, restoration activities should prioritise avoiding the use of non-native plant species.

Patch age alters seagrass response mechanisms to herbivory damage.

Natural disturbances can produce a mosaic of seagrass patches of different ages, which may affect the response to herbivory. These pressures can have consequences for plant performance. To assess how seagrass patch age affects the response to herbivory, we simulated the effect of herbivory by clipping leaves of Halodule wrightii in patches of 2, 4 and 6 years. All clipped plants showed ability to compensate herbivory by increasing leaf growth rate (on average 4.5-fold). The oldest patches showed resistance response by increasing phenolic compounds (1.2-fold). Contrastingly, the concentration of phenolics decreased in the youngest patches (0.26-fold), although they had a similar leaf carbon content to controls. These results suggest that younger plants facing herbivory pressure reallocate their phenolic compounds towards primary metabolism. Results confirm the H. wrightii tolerance to herbivory damage and provides evidence of age-dependent compensatory responses, which may have consequences for seagrass colonization and growth in perturbed habitats.

Environmental DNA metabarcoding reveals the effects of seafloor litter and trawling on marine biodiversity.

Environmental DNA (eDNA) techniques are emerging as promising tools for monitoring marine communities. However, they have not been applied to study the integrated effects of anthropogenic pressures on marine biodiversity. This study examined the relationships between demersal community species composition, key environmental features, and anthropogenic impacts such as fishing effort and seafloor litter using eDNA data in the central Tyrrhenian Sea. The results indicated that both fishing effort and seafloor litter influenced species composition and diversity. The adaptive traits of marine species played a critical role in their response to debris accumulation and fishing. Mobile species appeared to use relocation strategies, while sessile species showed flexibility in the face of disturbance. Epibiotic species relied on passive transport. The use of eDNA-based methods is a valuable resource for monitoring anthropogenic impacts during scientific surveys, enhancing our ability to monitor marine ecosystems and more effectively assess the effects of pollution.

Seafloor warm water temperature anomalies impact benthic macrofauna communities of a high-Arctic cold-water fjord.

Amid the alarming atmospheric and oceanic warming rates taking place in the Arctic, western fjords around the Svalbard archipelago are experiencing an increased frequency of warm water intrusions in recent decades, causing ecological shifts in their ecosystems. However, hardly anything is known about their potential impacts on the until recently considered stable and colder northern fjords. We analyzed macrobenthic fauna from four locations in Rijpfjorden (a high-Arctic fjord in the north of Svalbard) along its axis, sampled intermittently in the years 2003, 2007, 2010, 2013 and 2017. After a strong seafloor warm water temperature anomaly (SfWWTA) in 2006, the abundance of individuals and species richness dropped significantly across the entire fjord in 2007, together with diversity declines at the outer parts (reflected in Shannon index drops) and increases in beta diversity between inner and outer parts of the fjord. After a period of three years with stable water temperatures and higher sea-ice cover, communities recovered through recolonization processes by 2010, leading to homogenization in community composition across the fjord and less beta diversity. For the last two periods (2010-2013 and 2013-2017), beta diversity between the inner and outer parts gradually increased again, and both the inner and outer sites started to re-assemble in different directions. A few taxa began to dominate the fjord from 2010 onwards at the outer parts, translating into evenness and diversity drops. The inner basin, however, although experiencing strong shifts in abundances, was partially protected by a fjordic sill from impacts of these temperature anomalies and remained comparatively more stable regarding community diversity after the disturbance event. Our results indicate that although shifts in abundances were behind important spatio-temporal community fluctuations, beta diversity variations were also driven by the occurrence-based macrofauna data, suggesting an important role of rare taxa. This is the first multidecadal time series of soft-bottom macrobenthic communities for a high-Arctic fjord, indicating that potential periodic marine heatwaves might drive shifts in community structure, either through direct effects from thermal stress on the communities or through changes in environmental regimes led by temperature fluctuations (i.e. sea ice cover and glacial runoff, which could lead to shifts in primary production and food supply to the benthos). Although high-Arctic macrobenthic communities might be resilient to some extent, sustained warm water anomalies could lead to permanent changes in cold-water fjordic benthic systems.

The Invasion of Alien Populations of Solanum elaeagnifolium in Two Mediterranean Habitats Modifies the Soil Communities in Different Ways.

We aimed to explore how the invasion of the alien plant Solanum elaeagnifolium affects soil microbial and nematode communities in Mediterranean pines (Pinus brutia) and maquis (Quercus coccifera). In each habitat, we studied soil communities from the undisturbed core of both formations and from their disturbed peripheral areas that were either invaded or not by S. elaeagnifolium. Most studied variables were affected by habitat type, while the effect of S. elaeagnifolium was different in each habitat. Compared to maquis, the soil in pines had higher silt content and lower sand content and higher water content and organic content, supporting a much larger microbial biomass (PLFA) and an abundance of microbivorous nematodes. The invasion of S. elaeagnifolium in pines had a negative effect on organic content and microbial biomass, which was reflected in most bacterivorous and fungivorous nematode genera. Herbivores were not affected. In contrast, in maquis, organic content and microbial biomass responded positively to invasion, raising the few genera of enrichment opportunists and the Enrichment Index. Most microbivores were not affected, while herbivores, mostly Paratylenchus, increased. The plants colonizing the peripheral areas in maquis probably offered a qualitative food source to microbes and root herbivores, which in pines was not sufficient to affect the much larger microbial biomass.

Non-native species change the tune of tundra soils: Novel access to soundscapes of the Arctic earthworm invasion.

Over the last decade, an increasing number of studies have used soundscapes to address diverse ecological questions. Sound represents one of the few sources of information capable of providing in situ insights into processes occurring within opaque soil matrices. To date, the use of soundscapes for soil macrofauna monitoring has been experimentally tested only in controlled laboratory environments. Here we assess the validity of laboratory predictions and explore the use of soil soundscape proxies for monitoring soil macrofauna (i.e., earthworm) activities in an outdoor context. In a common garden experiment in northern Sweden, we constructed outdoor mesocosm plots (N = 36) containing two different Arctic vegetation types (meadow and heath) and introduced earthworms to half of these plots. Earthworms substantially altered the ambient soil soundscape under both vegetation types, as measured by both traditional soundscape indices and frequency band power levels, although their acoustic impacts were expressed differently in heath versus meadow soils. While these findings support the as-of-yet untapped promise of using belowground soundscape analyses to monitor soil ecosystem health, direct acoustic emissions from earthworm activities appear to be an unlikely proxy for tracking worm activities at daily timescales. Instead, earthworms indirectly altered the soil soundscape by 're-engineering' the soil matrix: an effect that was dependent on vegetation type. Our findings suggest that long-term (i.e., seasonal) earthworm activities in natural soil settings can likely be monitored indirectly via their impacts on soundscape measures and acoustic indices. Analyzing soil soundscapes may enable larger-scale monitoring of high-latitude soils and is directly applicable to the specific case of earthworm invasions within Arctic soils, which has recently been identified as a potential threat to the resilience of high-latitude ecosystems. Soil soundscapes could also offer a novel means to monitor soils and soil-plant-faunal interactions in situ across diverse pedogenic, agronomic, and ecological systems.

Ecological perspectives on water, food, and health security linkages: the Minamata case in Japan.

Extant studies address water, food, and health security issues considerably separately and within narrow disciplinary confines. This study investigates the links among these three issues from an ecological viewpoint with a multidisciplinary approach in a modified Millennium Ecosystem Assessment framework developed by the United Nations. The modified framework includes water, food, and health security considerations as the three constituents of human well-being from an ecological (more specifically, ecosystem services) viewpoint. This study examines the links through published data associated with the Minamata incident, which was a historic and horrific methylmercury-induced water, food, and health poisoning crisis in Japan. The results show that when heavy metal pollution changes one component (marine water) of the provisioning ecosystem services, this change subsequently affects another component (seafood) of the services. This then defines the linkages among water, food, and health security as the three constituents of human well-being within the modified framework. The links can have immediate and far-reaching economic, social, legal, ethical, and justice implications within and across generations. This study provides important evidence for emerging economies that ignore the water-food-health security nexus.

Evaluating multiple stressor research in coastal wetlands: A systematic review.

Multiple stressors are ubiquitous in coastal ecosystems as a result of increased human activity and development along coastlines. Accurately assessing multiple stressor effects is essential for predicting stressor impacts and informing management to efficiently and effectively mitigate potentially complex ecological responses. Extracting relevant information on multiple stressor studies conducted specifically within coastal wetlands is not possible from existing reviews, posing challenges in highlighting knowledge gaps and guiding future research. Here, we systematically review manipulative studies that assess multiple anthropogenic stressors within saltmarsh, mangrove, and seagrass ecosystems. In the past decade, there has been a rapid increase in publications, with seagrasses receiving the most attention (76 out of a total of 143 studies). Across all studies, nutrient loading and temperature were tested most often (N = 64 and N = 48, respectively), while the most common stressor combination was temperature with salinity (N = 12). Stressor application and study design varied across ecosystems. Studies are mostly conducted in highly controlled environments, without considering how natural variations in the physicochemical environment of coastal ecosystems may influence stressor intensity and timing under these conditions. This may result in vastly different ecological responses across levels of biological organisation. Shifting focus from univariate analytical approaches to multivariate, particularly path analysis, will help elucidate complex ecological relationships and highlight direct and indirect effects of multiple stressors in coastal ecosystems. There is a solid foundation of multiple stressor research in coastal wetlands. However, we recommend future research enhance ecological realism in experimental design by studying the effects of stressor combinations whilst accounting for spatiotemporal variability that reflects natural conditions of coastal ecosystems.

Cancer risk landscapes: A framework to study cancer in ecosystems.

Cancer is a family of diseases that has been documented in most metazoan species and ecosystems. Human induced environmental changes are increasingly exposing wildlife to carcinogenic risk factors, and negative repercussions on ecosystems and on the conservation of endangered species are already been observed. It is therefore of key importance to understand the spatiotemporal variability of those risk factors and how they interact with the biosphere to mitigate their effects. Here we introduce the concept of cancer risk landscape that can be applied to understand how species are exposed to, interact with, and modify cancer risk factors. With this publication we aim to provide a framework in order to stimulate a discussion on how to mitigate cancer-causing risk factors.

Quantitative data on latest-quaternary benthic foraminiferal assemblages in the South Evoikos Gulf semi-enclosed basin (central Aegean, Greece).

We herein present an original high-resolution dataset on the Late Pleistocene to Holocene (>15.17 ka BP) benthic foraminiferal record of two continuous sediment cores (DEH 5 and DEH 1), drilled from the shallow (max. depth ∼75 m) semi-enclosed basin of South Evoikos Gulf (central Aegean, Greece). Owing to its particular configuration, this marginal setting has been heavily affected by the latest-glacial to modern-interglacial sea-level and climate oscillations that left clear imprints on the benthic foraminiferal community. Our data comprise quantitative information of the downcore faunal distribution (raw species counts and relative abundances), diversity measurements, simplified datasets used for clustering analysis and calibrated age spans. This material can be efficiently utilized in any comparative or synthetic future study on the reconstruction of the latest-Quaternary palaeoceanographic (palaeobathymetric, sea-level) and palaeoenvironmental evolution of the Mediterranean shelf. The present data article is associated with the research article "Impact of latest-glacial to Holocene sea-level oscillations on central Aegean shelf ecosystems: A benthic foraminiferal palaeoenvironmental assessment of South Evoikos Gulf, Greece" by Louvari et al. (2019).

Deep-water fisheries along the British Isles continental slopes: status, ecosystem effects and future perspectives.

In this paper, we revisit the state of deep-water fisheries to the west of the British Isles and aim to provide an overview on the key drivers behind community changes along continental margins. The deep-water fisheries to the west of the British Isles that extend from the shelf-slope break down to the lower slope and along banks and seamounts of the Rockall Basin, mainly target blue ling Molva dypterygia, roundnose grenadier Coryphaenoides rupestris, orange roughy Hoplostethus atlanticus, with by-catches of black scabbardfish Aphanopus carbo and tusk Brosme brosme. These fishing grounds experienced a long period of exhaustive exploitation until the early 2000s, but subsequently the implementation of management strategies has helped to relieve excessive fishing pressure. It is widely accepted that a better understanding of the long-term implications of disturbance is needed to understand patterns in deep-water communities and what sustainable use and exploitation of resources might look like in this context.

Comparison of aboveground vegetation and soil seed bank composition at sites of different grazing intensity around a savanna-woodland watering point in West Africa.

Grazing removes a plant's aboveground vegetative and reproductive tissues and can modify the soil seed bank, potentially impacting the restoration of preferred species. Knowledge about aboveground vegetation and species composition of soil seed bank and the processes that contribute to vegetation recovery on and surrounding watering points subjected to grazing is lacking. Successful restoration strategies hinge on addressing these knowledge gaps. We assessed the effects of livestock grazing on aboveground vegetation and soil seed bank characteristics along a river bank and surrounding areas subject to different grazing intensities and draw implications for restoration. Plots (50 × 50 m) were established along five transects representing differing levels of grazing intensity. Soil samples were taken from three layers within each plot to determine soil properties and species composition of soil seed bank using the seedling emergence method. Heavy grazing resulted in the disappearance of perennial grasses, a reduction in species diversity and a decrease in soil nutrients with increased soil depth. Overall, the similarity between the extant aboveground vegetation and flora within the soil seed bank was low. The soil seed bank was dominated by herbaceous species and two woody species, suggesting that many woody species are not accumulating in the soil. With increasing soil depth, the seed density and richness declined. Canonical correspondence analyses (CCAs) showed that emerged seedlings from the soil seed bank were significantly influenced by soil carbon, organic matter, total nitrogen, total potassium and soil cation exchange capacity. This finding suggests that current grazing practices have a negative impact on the vegetation surrounding watering points; hence there is a need for improved grazing management strategies and vegetation restoration in these areas. The soil seed bank alone cannot restore degraded river banks; active transfer of propagules from adjacent undisturbed forest areas is essential.

Protection alone may not promote natural recovery of biogenic habitats of high biodiversity damaged by mobile fishing gears.

The horse mussel Modiolus modiolus (L.) is a large marine bivalve that aggregates to create complex habitats of high biodiversity. As a keystone species, M. modiolus is of great importance for the functioning of marine benthic ecosystems, forming biogenic habitats used to designate Marine Protected Areas (MPAs). The present study investigates the condition of M. modiolus beds historically subjected to intense scallop fishing using mobile fishing gears. The study, conducted seven years after the introduction of legislation banning all forms of fishing, aimed to establish whether natural habitat recovery occurs after protection measures are put in place. Lower biodiversity and up to 80% decline in densities of M. modiolus were recorded across the current distributional range of the species in Strangford Lough, Northern Ireland. The decline in biodiversity in most areas surveyed was consistent with that observed in biogenic reefs impacted by mobile fishing gears elsewhere. Epifauna, including sponges, hydroids and tunicates, experienced the most substantial decline in biodiversity, with up to 64% fewer taxa recorded in 2010 compared with 2003. Higher variability in community composition and a shift towards faunal assemblages dominated by opportunistic infaunal species typical of softer substrata were also detected. Based on these observations we suggest that, for biogenic habitats, the designation of MPAs and the introduction of fishing bans alone may not be sufficient to reverse or halt the negative effects caused by past anthropogenic impacts. Direct intervention, including habitat restoration based on translocation of native keystone species, should be considered as part of management strategies for MPAs which host similar biogenic reef habitats where condition and natural recovery have been compromised.

Applying fingerprint FTIR spectroscopy and chemometrics to assess soil ecosystem disturbance and recovery.

The assessment and monitoring of soil disturbance and its effect on soil quality (i.e., ability to support a range of ecosystem services) has been hindered due to the shortcomings of many traditional analytical techniques (e.g., soil enzyme activities, microbial incubations), including: high cost, long-term time investment and difficulties with data interpretation. Consequently, there is a critical need to develop a rapid and repeatable approach for quantifying changes in soil quality that will provide an assessment of the current status, condition and trend of natural and managed ecosystems. Here we report on a rapid, high-throughput approach to develop an ecological 'fingerprint' of a soil using Fourier transformed infrared (FTIR) spectroscopy and chemometric modeling, and its application to assess soil ecosystem status and trend. This methodology was applied in a highly disturbed forest ecosystem over a 19-year sampling period to detect changes in soil quality (detected via changes in spectral properties), resulting from changes in dynamic soil properties (e.g., soil organic matter, reactive mineralogy). Two chemometric statistical techniques (i.e., hierarchical clustering analysis and discriminate analysis of principal components) were evaluated for interpreting and quantifying similarities/dissimilarities between samples utilizing the entire FTIR spectra (i.e., fingerprint) from each sample. We found that this approach provided a means for clearly discriminating between degraded soils, soils in recovery and reference soils. Results from fingerprint FTIR analysis illustrate its power and potential for the monitoring and assessment of soil quality and soil landscape change.

Habitat associations of an expanding native alga.

There are many examples of native macrophytes becoming locally dominant and spreading outside their traditional distributions, but the causes and impacts are often not understood. In New South Wales, Australia, the green alga Caulerpa filiformis is undergoing a range expansion and has transitioned from a subdominant to a dominant alga on several rocky shores around the Sydney coastline. Here we investigated relationships between established patches of C. filiformis, the habitat it occupies and associated algal communities at multiple subtidal sites over the green alga's 700 km range. We tested the following predictions: 1) C. filiformis cover differs among substrata, being greatest on turf-forming algae; 2) C. filiformis cover is positively related to environmental variables linked to increased sedimentation (e.g. reduced reef width, surface slope, increased rugosity and distance from shore); 3) occurrence of C. filiformis is associated with a change in macrophyte community structure and a reduction of macrophyte richness; 4) intact native algal canopies inhibit C. filiformis spread, but turf-forming algae and bare sand are susceptible to invasion. Substratum associations were highly consistent among sites, but contrary to our prediction, C. filiformis was most commonly associated with rock or rock + sand substratum and less frequently associated with turf-forming algae substratum. C. filiformis cover was negatively correlated with reef width, which explained most of the variation observed, although local scale variables distance from shore, reef slope, and water depth were also correlated with C. filiformis cover. Algal diversity and community composition typically differed in the presence of C. filiformis, often with a reduction of algal abundances, in particular Sargassum spp., although results varied among substrata and sites. However, monitoring of borders suggested that C. filiformis does not invade and outcompete undisturbed adjacent canopy-forming algae over a 12 month period. Our results suggest that disturbance processes (possibly linked to sedimentation) acting at the site and quadrat scale are likely important determinants of C. filiformis cover and spread, and hence its potential ecological impacts.

Revealing turning points in ecosystem functioning over the Northern Eurasian agricultural frontier.

The collapse of the Soviet Union in 1991 has been a turning point in the World history that left a unique footprint on the Northern Eurasian ecosystems. Conducting large scale mapping of environmental change and separating between naturogenic and anthropogenic drivers is a difficult endeavor in such highly complex systems. In this research a piece-wise linear regression method was used for breakpoint detection in Rain-Use Efficiency (RUE) time series and a classification of ecosystem response types was produced. Supported by earth observation data, field data, and expert knowledge, this study provides empirical evidence regarding the occurrence of drastic changes in RUE (assessment of the timing, the direction and the significance of these changes) in Northern Eurasian ecosystems between 1982 and 2011. About 36% of the study area (3.4 million km(2) ) showed significant (P < 0.05) trends and/or turning points in RUE during the observation period. A large proportion of detected turning points in RUE occurred around the fall of the Soviet Union in 1991 and in the following years which were attributed to widespread agricultural land abandonment. Our study also showed that recurrent droughts deeply affected vegetation productivity throughout the observation period, with a general worsening of the drought conditions in recent years. Moreover, recent human-induced turning points in ecosystem functioning were detected and attributed to ongoing recultivation and change in irrigation practices in the Volgograd region, and to increased salinization and increased grazing intensity around Lake Balkhash. The ecosystem-state assessment method introduced here proved to be a valuable support that highlighted hotspots of potentially altered ecosystems and allowed for disentangling human from climatic disturbances.

Effects of kelp phenolic compounds on the feeding-associated mobility of the herbivore snail Tegula tridentata.

Tegula tridentata, is a common herbivore gastropod inhabiting the subtidal Lessonia trabeculata kelp forest, which tends to show higher densities after kelp harvesting. We investigated if harvested kelp beds may harbor higher densities of herbivore invertebrates, and the underlying mechanisms. Thus, we evaluated if the exudates of L. trabeculata change the seawater levels of soluble phenols, known to have a deterrent effect against the feeding behavior of some herbivore invertebrates. Finally we investigated whether the increase in T. tridentata densities in harvested kelp grounds could be related to a decrease in the seawater levels of soluble phenols. Our results showed that the density of invertebrate herbivores increased up to 32% in harvested kelp grounds. We provide the first estimate of the rate of phenolic exudation by L. trabeculata, and we demonstrate that T. tridentata changes its food dependent movement in the presence of exudates with synthetic phloroglucinol. We suggest that the recovery of harvested kelp ecosystems can be jeopardized by increased herbivory triggered by water-borne changes in the levels of herbivore deterrent compounds.

Twenty-year changes in coral near Muscat, Oman estimated from manta board tow observations.

The coastline of Muscat, Oman, contains some of the most extensive and diverse coral reefs in the northeastern Arabian Peninsula. In the past two decades this region has been impacted by expanding coastal development, the largest cyclone ever recorded in the Arabian Sea, and a large-scale harmful algal bloom which resulted in mass mortality of reefs elsewhere in the Gulf of Oman. In 2012 we estimated live and dead coral using manta tow observations on 370 transects at 13 locations along the coastline and nearshore islands of Muscat Oman. We compared these estimates against observations made on 389 transects at the same 13 locations two decades earlier (1993-94) in order to determine long-term changes in benthos along the Muscat coast. Results were mapped and differences in categorical mean values for transect locations were statistically compared between survey years. Live hard and soft coral decreased over the past two decades at most survey sites, and decreases were significant at three exposed coastline sites and one semi-enclosed embayment. One sheltered embayment site showed a significant increase in live hard coral over the study period. Declines in live hard coral were associated with increases in dead coral framework at 8 of the 13 sites, but these changes were non-significant. We attribute these changes primarily to long-term effects of Cyclone Gonu, which struck the Oman coast in June 2007. The study results suggest that the manta tow method can be an effective way to detect long-term changes in coral and other benthic parameters over large areas, despite limitations on its precision.