Safety and Performance of a Cell-Impermeable Endoprosthesis for Hemodialysis Vascular Access Outflow Stenosis: A Brazilian Multicenter Retrospective Study.

PURPOSE: To evaluate the safety and performance of Wrapsody™, a cell-impermeable endoprosthesis (CIE), for treating hemodialysis vascular access outflow stenosis. MATERIALS AND METHODS: Investigators retrospectively analyzed 113 hemodialysis patients treated with a CIE (11/2021-12/2022) across four centers in Brazil. De novo or restenotic lesions were treated. The primary efficacy outcome measure was target lesion primary patency (TLPP) at 1, 3, 6, and 12 months; the primary safety outcome measure was the absence of serious local or systemic adverse events within the first 30 days post-procedure. Secondary outcome measures included technical and procedural success, access circuit primary patency (ACPP), and secondary patency at 1, 3, 6, and 12 months post-procedure. RESULTS: Thirty-nine patients (34.5%) had thrombosed access at the initial presentation, and 38 patients (33.6%) presented with recurrent stenosis. TLPP rates at 1, 3, 6, and 12 months were 100%, 96.4%, 86.4%, and 69.7%, respectively. ACPP rates were 100% at 1 month, 89.2% at 3 months, 70.9% at 6 months, and 56.0% at 12 months. The target lesion secondary patency rates at 1, 3, 6, and 12 months were 100%, 97.3%, 93.6%, and 91.7%, respectively. In the adjusted multivariate Cox regression analysis, male sex and endoprosthesis with diameters of 10, 12, 14, and 16 mm were associated with improved primary patency rates. No localized or systemic serious adverse event was observed through the first 30 days post-procedure. CONCLUSION: The CIE evaluated in this study is safe and effective for treating peripheral and central outflow stenoses in hemodialysis vascular access. LEVEL OF EVIDENCE: Level 2b, cohort study.

Water Colour Shapes Diving Beetle (Coleoptera: Dytiscidae) Assemblages in Urban Ponds.

Dramatic land-use changes in urban landscapes can drive water colour darkening by washing compounds, such as organic matter and iron, from terrestrial ecosystems into urban blue space, consequentially affecting aquatic communities. Here, I studied how pond water colour changes along an urban gradient and how diving beetles (Dytiscidae) respond to the water colour gradient in 11 ponds with fish and 15 ponds without fish in the Helsinki Metropolitan Area, Finland. I found that the pond water colour exhibited a non-significant decreasing pattern along the urban gradient, indicating that urbanisation may not necessarily drive brownification in urban ponds. Dytiscid species richness and abundance exhibited significant positive correlations with increasing water colour in ponds with fish but no significant correlation in ponds without fish. Some species, such as Agabus spp. and Dytiscus spp., appeared tolerant to highly coloured water, whereas some species, such as Hyphydrus ovatus and Hygrotus spp., tended to occur in clear water, indicating that brown water may provide dytiscids with prey refuges, but some species are intolerant to brown water. The study highlights the importance of urban pondscape heterogeneity to meet the needs of aquatic invertebrates that prefer different water colours and for the multifunctioning of urban ponds.

Single-Cell Oral Delivery Platform for Enhanced Acid Resistance and Intestinal Adhesion.

Oral delivery of cells, such as probiotics and vaccines, has proved to be inefficient since cells are generally damaged in an acidic stomach prior to arrival at the intestine to exert their health benefits. In addition, short retention in the intestine is another obstacle which affects inefficiency. To overcome these obstacles, a cell-in-shell structure was designed with pH-responsive and mucoadhesive properties. The pH-responsive shell consisting of three cationic layers of chitosan and three anionic layers of trans-cinnamic acid (t-CA) was made via layer-by-layer (LbL) assembly. t-CA layers are hydrophobic and impermeable to protons in acid, thus enhancing cell gastric resistance in the stomach, while chitosan layers endow strong interaction between the cell surface and the mucosal wall which facilitates cell mucoadhesion in the intestine. Two model cells, probiotic L. rhamnosus GG and dead Streptococcus iniae, which serve as inactivated whole-cell vaccine were chosen to test the design. Increased survival and retention during oral administration were observed for coated cells as compared with naked cells. Partial removal of the coating (20-60% removal) after acid treatment indicates that the coated vaccine can expose its surface immunogenic protein after passage through the stomach, thus facilitating vaccine immune stimulation in the intestine. As a smart oral delivery platform, this design can be extended to various macromolecules, thus providing a promising strategy to formulate oral macromolecules in the prevention and treatment of diseases at a cellular level.

Decoding the decisive role of seed moisture content in physical dormancy break: filling the missing links.

Species producing seeds with a water-impermeable seed coat, i.e., physical dormancy (PY), dominate the dry tropical forests. Despite increasing interest and understanding of the germination ecology of a PY species, less is known about how PY break occurs, particularly what changes lead to the opening of the 'water gap'. Based on the moisture conent (MC) attained, two ranges of PY may exist: shallow PY, a state with higher MC and seeds could reverse to a permeable state when the relative humidity increases; and absolute PY, a completely dry state. Here, we demonstrate that this MC variation between seeds affects preconditioning and the 'water-gap' opening stages. A conceptual model developed shows a strong relationship between temperature and duration, with high temperature breaking PY in seconds, but seasonal temperature fluctuations and constant temperatures require a longer time. The duration required at any conditions to break PY is purported to depend on the hydrophobic bonds of the lipids, which are likely weakened during the preconditioning, and the amount of water influences hydrolysis, leading to the 'water-gap' opening. We argue that the moisture content of the seeds and its interaction with biochemical compounds are a possible explanation for why only a proportion of PY seeds become permeable to water each year. Nonetheless, empirical investigations must validate these notions.

Organic fluorescent probes for live-cell super-resolution imaging.

The development of super-resolution technology has made it possible to investigate the ultrastructure of intracellular organelles by fluorescence microscopy, which has greatly facilitated the development of life sciences and biomedicine. To realize super-resolution imaging of living cells, both advanced imaging systems and excellent fluorescent probes are required. Traditional fluorescent probes have good availability, but that is not the case for probes for live-cell super-resolution imaging. In this review, we first introduce the principles of various super-resolution technologies and their probe requirements, then summarize the existing designs and delivery strategies of super-resolution probes for live-cell imaging, and finally provide a brief conclusion and overview of the future.

Influence of landscape patterns on nitrate and particulate organic nitrogen inputs to urban stormwater runoff.

This study investigated the effect of the landscape pattern of permeable/impermeable patches on NO3--N and particulate organic nitrogen (PON) concentrations during stormwater runoff transport and their source contributions. Six landscape pattern indices, namely, mean proximity index (MPI), largest patch index (LPI), mean shape index (MSI), landscape shape index (LSI), connect index (CONNECT), and splitting index (SPLIT), were selected to reflect the fragmentation, complexity, and connectivity of permeable patches in urban catchments. The results show that lower fragmentation, higher complexity, and greater connectivity can reduce NO3--N concentrations in road runoff and drainage flow (i.e., the flow in the stormwater drainage network), as well as PON concentrations in road runoff. Further, the above landscape pattern is effective for mitigating the contributions of NO3--N and PON from road runoff. Low impact development (LID) can be incorporated with the landscape pattern of permeable/impermeable patches to mitigate nitrogen pollution in urban stormwater at the catchment scale by optimizing the spatial arrangement.

Cell-Impermeable Inhibitors Confirm That Intracellular Human Transglutaminase 2 Is Responsible for the Transglutaminase-Associated Cancer Phenotype.

Transglutaminase 2 (TG2) is a multifunctional enzyme primarily responsible for crosslinking proteins. Ubiquitously expressed in humans, TG2 can act either as a transamidase by crosslinking two substrates through formation of an Nε(ɣ-glutaminyl)lysine bond or as an intracellular G-protein. These discrete roles are tightly regulated by both allosteric and environmental stimuli and are associated with dramatic changes in the conformation of the enzyme. The pleiotropic nature of TG2 and multi-faceted activities have resulted in TG2 being implicated in numerous disease pathologies including celiac disease, fibrosis, and cancer. Targeted TG2 therapies have not been selective for subcellular localization, such that currently no tools exist to selectively target extracellular over intracellular TG2. Herein, we have designed novel TG2-selective inhibitors that are not only highly potent and irreversible, but also cell impermeable, targeting only extracellular TG2. We have also further derivatized the scaffold to develop probes that are intrinsically fluorescent or bear an alkyne handle, which target both intra- and extracellular TG2, in order to facilitate cellular labelling and pull-down assays. The fluorescent probes were internalized and imaged in cellulo, and provide the first implicit experimental evidence that by comparison with their cell-impermeable analogues, it is specifically intracellular TG2, and presumably its G-protein activity, that contributes to transglutaminase-associated cancer progression.

Prospective, Multicenter, Observational Study to Evaluate a Cell-Impermeable Endoprosthesis for Treatment of Stenosis or Occlusion within the Dialysis Outflow Circuit of an Arteriovenous (AV) Fistula or AV Graft (The WRAP Registry).

PURPOSE: Dysfunctional vascular access due to stenosis/occlusion within the arteriovenous fistula or graft (AVF/AVG) negatively affects the clinical management of patients undergoing hemodialysis. Results from the feasibility study of the WRAPSODY™ Cell-Impermeable Endoprosthesis demonstrated that the device can achieve high patency rates and maintain integrity of the dialysis outflow circuit. This study was designed to assess real-world evidence of safety and efficacy outcomes following device placement. MATERIALS AND METHODS: This is a prospective, multicenter, non-investigational, post-market observational study of 500 patients at up to 50 centers worldwide with a mature AVF/AVG dialysis access (≥ 1 hemodialysis session) who experience stenosis/occlusion of the outflow circuit prior to placement of WRAPSODY. Patients will be divided into the following two cohorts: peripheral or central thoracic. Primary outcome measures include target lesion primary patency (TLPP) at 6 months and procedure and/or device-related events through 30 days post-procedure. Secondary outcome measures include TLPP, safety events, and the number of interventions needed to maintain patency through the 24 month study period. Exploratory endpoints include time to access abandonment, resumption of successful dialysis, functional patency, and pending available imaging data, any incidence of stent fractures, migration, or edge stenosis. Study enrollment began in June 2022, the last patient visit is expected in 2026. DISCUSSION: It is expected that this study will provide real-world evidence regarding the performance of the WRAPSODY device in a diverse population of patients, which may encourage its use in the continuum of hemodialysis access management. TRIAL REGISTRATION: NCT05062291.

Investigating the relationship between COVID-19 shutdown and land surface temperature on the Anatolian side of Istanbul using large architectural impermeable surfaces.

Artificial impermeable surfaces are becoming more prevalent, especially in urban areas, as a result of shifting land use and cover, roads, roofs, etc. The modification of land surface temperature (LST) can also be accomplished through artificially impermeable surfaces. Large artificial impermeable surfaces, such as rooftops, parking lots, and other areas of use, can be found in industrial zones, shopping malls, industrial airports, and other locations. For the Anatolian side of Istanbul, 14 Landsat 8 OLI/TIRS imagery images over the years 2016-2022 were investigated. To evaluate how well the study's images could be utilized, correlation and cosine similarity approaches were employed. A total of 12 images may be employed for research LST studies, it was discovered. We looked at closure dates during the COVID-19 epidemic to find out how human migration affected the LST. In addition, the LST value was estimated using the ordinary least squares (OLS) method employing LST and other biophysical indices. A decrease in LST values was seen as a result of the investigation. High levels of similarity and correlation were found between the images used. Results from the Google Mobility Index also provide support to the study. All of these facts provide support to Istanbul's Anatolian side experiencing lower surface temperature values, which consequently affects the city's massive structures.

Membrane Permeability Is Required for the Vasodilatory Effect of Carbonic Anhydrase Inhibitors in Porcine Retinal Arteries.

It has been demonstrated previously that a variety of carbonic anhydrase inhibitors (CAIs) can induce vasodilation in pre-contracted retinal arteriolar segments although with different efficacy and potency. Since the CAIs tested so far are able to permeate cell membranes and inhibit both intracellular and extracellular isoforms of the enzyme, it is not clear whether extra- or intracellular isoforms or mechanisms are mediating their vasodilatory effects. By means of small wire myography, we have tested the effects of four new CAIs on wall tension in pre-contracted retinal arteriolar segments that demonstrably do not enter cell membranes but have high affinity to both cytosolic and membrane-bound isoforms of CA. At concentrations between 10-6 M to 10-3 M, none of the four membrane impermeant CAIs had any significant effect on arteriolar wall tension, while the membrane permeant CAI benzolamide (10-3 M) fully dilated all arteriolar segments tested. This suggests that CAI act as vasodilators through cellular mechanisms located in the cytoplasm of vascular cells.

Characteristics of thermal pollution from stormwater runoff from impermeable/permeable pavement surfaces via a lab-scale experiment.

Thermal pollution from stormwater runoff has been the focus of many studies in recent years due to its potential harm to aquatic microorganisms. However, there were few studies on the thermal pollution caused by stormwater runoff from various types of urban pavement surfaces. A lab-scale experiment was conducted to compare the thermal load of stormwater runoff from impermeable and permeable pavements and the influencing factors were investigated. The experimental findings demonstrated that the rainfall return period and initial temperature of various pavement surfaces significantly impacted the thermal load. The stormwater runoff absorbed more heat as the initial temperature, and rainfall return period increased. The difference of the thermal load of stormwater runoff between permeable brick pavement (PBP) and the impermeable asphalt pavement (IAP) increased from 305.26 to 436.70 kJ/m2, when the initial surface temperature rose from 35 to 47 °C. The average runoff temperature decreased by 1.39-1.90 °C for PBP compared to the IAP, with an increase in surface temperature from 35 to 47 °C. Under the various initial surface temperatures, the mean temperature of the infiltration effluent from the PBP was 3.12-4.20 °C lower than the average temperature of stormwater runoff from the surface layer. Therefore, a PBP can effectively alleviate thermal pollution from stormwater runoff and safeguard the receiving waters' quality.

Stretchable Gas Barrier Films Using Liquid Metal toward a Highly Deformable Battery.

Highly deformable batteries that are flexible and stretchable are important for the next-generation wearable devices. Several studies have focused on the stable operation and life span of batteries. On the other hand, there has been less focus on the packaging of highly deformable batteries. In wearable devices, solid-state or pouch lithium-ion batteries (LIBs) packaged in aluminum (Al)-laminated films, which protect against moisture and gas permeation, are used. Stretchable elastomer materials are used as the packaging films of highly deformable batteries; however, they are extremely permeable to gas and moisture. Therefore, a packaging film that provides high deformability along with gas and moisture barrier functionalities is required for the stable operation of highly deformable batteries used in ambient conditions. In this study, a stretchable packaging film with high gas barrier functionality is developed successfully by coating a thin layer of liquid metal onto a gold (Au)-deposited thermoplastic polyurethane film using the layer-by-layer method. The film exhibits excellent oxygen gas impermeability under mechanical strain and extremely low moisture permeability. It shows high impermeability along with high mechanical robustness. Using the proposed stretchable gas barrier film, a highly deformable LIB is assembled, which offers reliable operation in air. The operation of the highly deformable battery is analyzed by powering LEDs under mechanical deformations in ambient conditions. The proposed stretchable packaging film can potentially be used for the development of packaging films in advanced wearable electronic devices.

Unravelling the paradox in physically dormant species: elucidating the onset of dormancy after dispersal and dormancy-cycling.

BACKGROUND: For species that produce seeds with a water-impermeable coat, i.e. physical dormancy (PY), it has been widely recognized that (1) seeds shed at a permeable state cannot become impermeable after dispersal; and (2) dormancy-cycling, i.e. a permeable ↔ impermeable transition, does not occur. Given a tight relationship between moisture content and onset of seed-coat impermeability, seeds maturing at low relative humidity (RH) and occurring in a high-temperature environment are inferred to produce impermeable coats, and ex situ drying of permeable seeds can lead to the onset of impermeability. SCOPE AND CONCLUSION: It is proposed here that permeable seeds dispersed at low RH and in high-temperature soils might become impermeable due to continuous drying. Similarly, seeds with shallow PY dormancy (with higher moisture content immediately after becoming impermeable) can cycle back to a permeable state or absolute PY (complete dry state) when RH increases or decreases, respectively. A conceptual model is developed to propose that seeds from several genera of 19 angiosperm families at the time of natural dispersal can be (1) impermeable (dormant), i.e. primary dormancy; (2) impermeable (dormant) and become permeable (non-dormant) and then enter a dormant state in the soil, often referred to as secondary dormancy; (3) permeable (non-dormant) and become impermeable (dormant) in the soil, i.e. enforced dormancy; or (4) dormant or non-dormant, but cycle between permeable and non-permeable states depending on the soil conditions, i.e. dormancy-cycling, which is different from sensitivity-cycling occurring during dormancy break. It is suggested that this phenomenon could influence the dormancy-breaking pattern, but detailed studies of this are lacking.

Nitrobenzoxadiazole derivatives of the rat selective toxicant norbormide as fluorescent probes for live cell imaging.

Norbormide [5-(α-hydroxy-α-2-pyridylbenzyl)-7-(α-2-pyridylbenzylidene)-5-norbornene-2,3-dicarboximide] (NRB, 1), an existing but infrequently used rodenticide, is known to be uniquely toxic to rats, but relatively harmless to other rodents and mammals. As a vasoactive agent, NRB induces a species-specific vasocontractile effect that is restricted to the peripheral arteries of the rat. Despite the precise mechanisms behind this phenomenon having yet to be fully clarified, it is postulated that the molecular target of NRB could be located within the plasma membrane of rat peripheral artery myocytes (e.g. rat caudal artery myocytes). As such, the primary objective of this study was to develop a fluorescently labelled derivative of NRB to investigate its subcellular distribution/localization in both NRB-sensitive (freshly isolated rat caudal artery myocytes, FIRCAMs) and NRB-insensitive (human hepatic stellate, LX2) cells. Of the examples prepared, lead structure endo-NRB-NBD-bPA subsequently demonstrated retention of the parent toxicant's pharmacological profile (in terms of its ability to induce both a vasocontractile response in rat caudal artery rings in vitro, and a lethal end-point in rats in vivo). Endo-NRB-NBD-bPA was also shown to be significantly less permeable (an integral feature in the design of fluorescent probes targeting cell-surface receptors) to both LX2 cells and FIRCAMs. Disappointingly, no fluorescence could be observed on the plasma membrane of FIRCAMs stained with endo-NRB-NBD-bPA.

Effects of an impermeable layer on pore pressure response to tsunami-like inundation.

Tsunami hazards have been observed to cause soil instability resulting in substantial damage to coastal infrastructure. Studying this problem is difficult owing to tsunamis' transient, non-uniform and large loading characteristics. To create realistic tsunami conditions in a laboratory environment, we control the body force using a centrifuge facility. With an apparatus specifically designed to mimic tsunami inundation in a scaled-down model, we examine the effects of an embedded impermeable layer on soil instability: the impermeable layer represents a man-made pavement, a building foundation, a clay layer and alike. The results reveal that the effective vertical soil stress is substantially reduced at the underside of the impermeable layer. During the sudden runup flow, this instability is caused by a combination of temporal dislocation of soil grains and an increase in pore pressure under the impermeable layer. The instability during the drawdown phase is caused by the development of excess pore-pressure gradients, and the presence of the impermeable layer substantially enhances the pressure gradients leading to greater soil instability. The laboratory results demonstrate that the presence of an impermeable layer plays an important role in weakening the soil resistance under tsunami-like rapid runup and drawdown processes.

Impermeable Graphene Oxide Protects Silicon from Oxidation.

The presence of a natural silicon oxide (SiOx) layer over the surface of silicon (Si) has been a roadblock for hybrid semiconductor and organic electronics technology. The presence of an insulating oxide layer is a limiting operational factor, which blocks charge transfer and therefore electrical signals for a range of applications. Etching the SiOx layer by fluoride solutions leaves a reactive Si-H surface that is only stable for few hours before it starts reoxidizing under ambient conditions. Controlled passivation of silicon is also of key importance for improving Si photovoltaic efficiency. Here, we show that a thin layer of graphene oxide (GOx) prevents Si surfaces from oxidation under ambient conditions for more than 30 days. In addition, we show that the protective GOx layer can be modified with molecules enabling a functional surface that allows for further chemical conjugation or connections with upper electrodes, while preserving the underneath Si in a nonoxidized form. The GOx layer can be switched electrochemically to reduced graphene oxide, allowing the development of a dynamic material for molecular electronics technologies. These findings demonstrate that 2D materials are alternatives to organic self-assembled monolayers that are typically used to protect and tune the properties of Si and open a realm of possibilities that combine Si and 2D materials technologies.

Does the common topknot Zeugopterus punctatus (Teleosteii: Pleuronectiformes: Scophthalmidae) use a novel Venturi-effect attachment mechanism? A testable hypothesis.

Common topknots (Zeugopterus punctatus) attach to vertical rock surfaces and overhangs. It has been speculated that attachment is by a suction cup, with the median (anal, dorsal) fins providing a peripheral seal. Here the authors propose that the attachment is actually based on a Venturi effect. The rear portions of the median fins continually move in a fan-like fashion (at c. 4 cycles per second). This movement produces a tailward fluid flow that ventilates the shallow underbody space between the fish and its rocky substratum. The anterior portions of the median fins seal the space laterally, but the space is open anterior (beneath the raised head) and posterior to the sea. The mid-underbody space likely has a lower cross-sectional area than does the front intake or rear exit, so flow should be faster (and pressure lower) within it than outside, thus providing pressure gradient suction. Topknots attach to rough and heavily biofouled surfaces, presumably because the high numbers of fin rays and their associated membranes plus fine muscle control allow effective sealing. The attachment ability is shared by all members of the flatfish tribe Phrynorhombini; it can be related to anatomical peculiarities and constitutes a probable synapomorphy for this clade.

Analytical Solution to Assess the Induced Seismicity Potential of Faults in Pressurized and Depleted Reservoirs.

Displaced faults crossing the reservoir could significantly increase the induced earthquake frequency in geo-energy projects. Understanding and predicting the stress variation in such cases is essential to minimize the risk of induced seismicity. Here, we adopt the inclusion theory to develop an analytical solution for the stress response to pore pressure variations within the reservoir for both permeable and impermeable faults with offset ranging from zero to the reservoir thickness. By analyzing fault stability changes due to reservoir pressurization/depletion under different scenarios, we find that (1) the induced seismicity potential of impermeable faults is always larger than that of permeable faults under any initial and injection conditions-the maximum size of the fault undergoing failure is 3-5 times larger for impermeable than for permeable faults; (2) stress concentration at the corners results in the occurrence of reversed slip in normal faults with a normal faulting stress regime; (3) while fault offset has no impact on the slip potential for impermeable faults, the slip potential increases with the offset for permeable faults, which indicates that non-displaced permeable faults constitute a safer choice for site selection; (4) an impermeable fault would rupture at a lower deviatoric stress, and at a smaller pressure buildup than a permeable one; and (5) the induced seismicity potential is overestimated and the injectivity underestimated if the stress arching (i.e., the poromechanical coupling) is neglected. This analytical solution is a useful tool for site selection and for supporting decision making during the lifetime of geo-energy projects.

Experimental Warming Hastens Physical Dormancy Break and Germination in Tropical Fabaceae.

Climate warming may threaten the germination strategies of many plants that are uniquely adapted to today's climate. For instance, species that employ physical dormancy (PY) - the production of seeds that are impermeable to water until high temperatures break them, consequently synchronizing germination with favorable growing conditions - may find that their seeds germinate during unfavorable or potentially fatal periods if threshold temperatures are reached earlier in the year. To explore this, we subjected the seeds of five species with physical dormancy (from the genera Abrus, Bauhinia, Cassia, Albizia, and Acacia) to "mild" (+2°C) and "extreme" (+4°C) future warming scenarios and documented their germination over 2 years relative to a control treatment. Under current climatic conditions, a proportion of seeds from all five species remained dormant in the soil for 2 years. A mild warming of 2°C had little to no effect on the germination of four of the five study species. Contrastingly, an extreme warming of 4°C dramatically increased germination in all five species within the first year, indicating a reduction in their ability to persist in the soil long-term. Cassia fistula was particularly susceptible to warming, exhibiting a similar increase in germination under both mild and extreme warming relative to control. Our findings suggest that climate warming in the tropics may cause the seeds of species that rely on physical dormancy to stagger the risk of unsuccessful germination across years to leave soil seed banks prematurely - the long-term implications of which remain unknown.

The influence of urban surface type and characteristics on runoff water quality.

Untreated runoff was collected over multiple rain events from 19 impermeable urban surfaces, including nine roofs, six roads and four carparks, to quantify the differences in water quality due to surface type, age, condition and location. All 19 sites were exposed to the same climatic conditions. Samples were analysed for key urban pollutants of concern, namely total suspended solids and total and dissolved copper and zinc. Results showed uncoated zinc-based roofs produced zinc concentrations (up to 55 mg/L) several orders of magnitude higher than receiving environment water quality guidelines in New Zealand, of which the vast majority was in dissolved form. Even non-metallic roofs with zinc-based guttering produced zinc concentrations over ten times higher than the same roof material without zinc-based guttering. Older zinc-based roofs had approximately five times higher zinc concentrations, demonstrating a substantial age effect on the untreated runoff quality. Similarly, copper roofs produced more than an order of magnitude higher copper concentrations (up to 7.8 mg/L) above the next highest copper-producing surfaces: higher trafficked roads and carparks. Regardless of traffic volume or function, all roads and carparks produced high TSS concentrations. Dissolved metal concentrations were high across the dataset confirming that metal partitioning is an important consideration for effective pollutant control as different removal processes need to be used for dissolved versus particulate metals. This dataset provides an important benchmark of untreated runoff quality across different impermeable surface types within the same geographical area and clearly shows the influence of surface characteristics on water quality runoff regardless of the local differences in land use. These findings provide valuable guidance to stormwater managers in identifying priority surfaces and selection of appropriate treatment strategies for effective stormwater management for total suspended solids, zinc and copper.