RESUMEN
A weak CH/O hydrogen-bonded organic framework (HOF) with both rigidity and flexibility that could easily and reversibly switch from a non-crystalline to a crystalline phase was constructed. The specific solvent molecule acts as a "key" to control the crystallinity, while the highly rigid triangle macrocycle as the building block is the "lock". The introduction and removal of the "key" could influence the local flexibility of the whole framework and lead to switchable crystallinity. Furthermore, the obtained HOF exhibits excellent separation efficiency for benzene and cyclohexane (94.4 %).
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To achieve the European climate targets and the Paris Agreements, at least 65% of the electricity needs to be generated from renewable energy sources by 2030. This requires a significant increase of distributed energy resources, posing a challenge for distribution system operators to integrate them into existing hierarchical grids. The concept of Local Flexibility Markets has recently gained attention as a market-based tool to tackle this challenge, making use of demand side flexibility. In this paper a Delphi method has been performed, showing that there are still numerous barriers in place preventing a widespread adoption of such markets in Europe. The main obstacles for market participants refer to standardisation issues. Based on that, a hybrid market model has been developed, comprising elements of a Local Flexibility Market and a Local Energy Market. To activate demand side flexibility from local energy transactions, spatio-temporally varying price signals are introduced, reflecting the constraints of the distribution grid. The paper shows, that this novel market approach helps to overcome relevant standardisation issues, but also certain barriers regarding end-users' lifestyles, which is because prices are comprehensible signals that can motivate end-users to participate. Moreover, a set of numerical examples is provided to illustrate the monetary benefits that could be gained by consumers and prosumers in the proposed hybrid market model. The examples show that the major share of the cost savings result from local energy trading, but the hybrid market model is also able to accumulate additional smaller revenues from providing flexibility. Finally, the systematic approach of characterising the market model in this paper offers a valuable framework for other researchers to map their ideas among existing approaches of Local Energy and Flexibility Markets.
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SGNH-type acetyl xylan esterases (AcXEs) play important roles in marine and terrestrial xylan degradation, which are necessary for removing acetyl side groups from xylan. However, only a few cold-adapted AcXEs have been reported, and the underlying mechanisms for their cold adaptation are still unknown because of the lack of structural information. Here, a cold-adapted AcXE, AlAXEase, from the Arctic marine bacterium Arcticibacterium luteifluviistationis SM1504T was characterized. AlAXEase could deacetylate xylooligosaccharides and xylan, which, together with its homologs, indicates a novel SGNH-type carbohydrate esterase family. AlAXEase showed the highest activity at 30 °C and retained over 70% activity at 0 °C but had unusual thermostability with a Tm value of 56 °C. To explain the cold adaption mechanism of AlAXEase, we next solved its crystal structure. AlAXEase has similar noncovalent stabilizing interactions to its mesophilic counterpart at the monomer level and forms stable tetramers in solutions, which may explain its high thermostability. However, a long loop containing the catalytic residues Asp200 and His203 in AlAXEase was found to be flexible because of the reduced stabilizing hydrophobic interactions and increased destabilizing asparagine and lysine residues, leading to a highly flexible active site. Structural and enzyme kinetic analyses combined with molecular dynamics simulations at different temperatures revealed that the flexible catalytic loop contributes to the cold adaptation of AlAXEase by modulating the distance between the catalytic His203 in this loop and the nucleophilic Ser32. This study reveals a new cold adaption strategy adopted by the thermostable AlAXEase, shedding light on the cold adaption mechanisms of AcXEs.
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Acetilesterasa/química , Acetilesterasa/metabolismo , Adaptación Fisiológica , Frío , Acetilesterasa/antagonistas & inhibidores , Acetilesterasa/genética , Secuencia de Aminoácidos , Bacterias/enzimología , Dominio Catalítico , Inhibidores Enzimáticos/farmacología , Estabilidad de Enzimas/efectos de los fármacos , Cinética , Metales/farmacología , Modelos Moleculares , Simulación de Dinámica Molecular , Mutación/genética , Filogenia , Multimerización de Proteína , Especificidad por Sustrato/efectos de los fármacos , TemperaturaRESUMEN
We systematically predict the internal flexibility of the S3 segment, one of the most mobile elements in the voltage-sensor domain. By analyzing the primary amino acid sequences of V-sensor containing proteins, including Hv1, TPC channels and the voltage-sensing phosphatases, we established correlations between the local flexibility and modes of activation for different members of the VGIC superfamily. Taking advantage of the structural information available, we also assessed structural aspects to understand the role played by the flexibility of S3 during the gating of the pore. We found that S3 flexibility is mainly determined by two specific regions: (1) a short NxxD motif in the N-half portion of the helix (S3a), and (2) a short sequence at the beginning of the so-called paddle motif where the segment has a kink that, in some cases, divide S3 into two distinct helices (S3a and S3b). A good correlation between the flexibility of S3 and the reported sensitivity to temperature and mechanical stretch was found. Thus, if the channel exhibits high sensitivity to heat or membrane stretch, local S3 flexibility is low. On the other hand, high flexibility of S3 is preferentially associated to channels showing poor heat and mechanical sensitivities. In contrast, we did not find any apparent correlation between S3 flexibility and voltage or ligand dependence. Overall, our results provide valuable insights into the dynamics of channel-gating and its modulation.
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Eucariontes/metabolismo , Canales Iónicos/química , Canales Iónicos/metabolismo , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Animales , Eucariontes/química , Eucariontes/clasificación , Eucariontes/genética , Activación del Canal Iónico , Canales Iónicos/genética , Ligandos , Filogenia , Conformación Proteica , Alineación de SecuenciaRESUMEN
In the preceding article, we present a flexibility analysis of the voltage-gated ion channel (VGIC) superfamily. In this study, we describe in detail the flexibility profile of the voltage-sensor domain (VSD) and the pore domain (PD) concerning the evolution of 6TM ion channels. In particular, we highlight the role of flexibility in the emergence of CNG channels and describe a significant level of sequence similarity between the archetypical VSD and the TolQ proteins. A highly flexible S4-like segment exhibiting Lys instead Arg for these membrane proteins is reported. Sequence analysis indicates that, in addition to this S4-like segment, TolQ proteins also show similarity with specific motifs in S2 and S3 from typical V-sensors. Notably, S3 flexibility profiles from typical VSDs and S3-like in TolQ proteins are also similar. Interestingly, TolQ from early divergent prokaryotes are comparatively more flexible than those in modern counterparts or true V-sensors. Regarding the PD, we also found that 2TM K+-channels in early prokaryotes are considerably more flexible than the ones in modern microbes, and such flexibility is comparable to the one present in CNG channels. Voltage dependence is mainly exhibited in prokaryotic CNG channels whose VSD is rigid whereas the eukaryotic CNG channels are considerably more flexible and poorly V-dependent. The implication of the flexibility present in CNG channels, their sensitivity to cyclic nucleotides and the cation selectivity are discussed. Finally, we generated a structural model of the putative cyclic nucleotide-modulated ion channel, which we coined here as AqK, from the thermophilic bacteria Aquifex aeolicus, one of the earliest diverging prokaryotes known. Overall, our analysis suggests that V-sensors in CNG-like channels were essentially rigid in early prokaryotes but raises the possibility that this module was probably part of a very flexible stator protein of the bacterial flagellum motor complex.
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Bacterias/metabolismo , Proteínas Bacterianas/química , Canales Catiónicos Regulados por Nucleótidos Cíclicos/química , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Aquifex , Bacterias/química , Bacterias/genética , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Canales Catiónicos Regulados por Nucleótidos Cíclicos/genética , Canales Catiónicos Regulados por Nucleótidos Cíclicos/metabolismo , Evolución Molecular , Ligandos , Familia de Multigenes , Nucleótidos Cíclicos/química , Nucleótidos Cíclicos/metabolismo , Dominios Proteicos , Alineación de SecuenciaRESUMEN
The histone-like (HU) protein is one of the major nucleoid-associated proteins of the bacterial nucleoid, which shares high sequence and structural similarity with IHF but differs from the latter in DNA-specificity. Here, we perform an analysis of structural-dynamic properties of HU protein from Spiroplasma melliferum and compare its behavior in solution to that of another mycoplasmal HU from Mycoplasma gallisepticum. The high-resolution heteronuclear NMR spectroscopy was coupled with molecular-dynamics study and comparative analysis of thermal denaturation of both mycoplasmal HU proteins. We suggest that stacking interactions in two aromatic clusters in the HUSpm dimeric interface determine not only high thermal stability of the protein, but also its structural plasticity experimentally observed as slow conformational exchange. One of these two centers of stacking interactions is highly conserved among the known HU and IHF proteins. Second aromatic core described recently in IHFs and IHF-like proteins is considered as a discriminating feature of IHFs. We performed an electromobility shift assay to confirm high affinities of HUSpm to both normal and distorted dsDNA, which are the characteristics of HU protein. MD simulations of HUSpm with alanine mutations of the residues forming the non-conserved aromatic cluster demonstrate its role in dimer stabilization, as both partial and complete distortion of the cluster enhances local flexibility of HUSpm.
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Proteínas Bacterianas/metabolismo , Proteínas de Unión al ADN/metabolismo , Fenilalanina/metabolismo , Spiroplasma/metabolismo , Secuencia de Aminoácidos , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Proteínas de Unión al ADN/química , Proteínas de Unión al ADN/genética , Espectroscopía de Resonancia Magnética , Simulación de Dinámica Molecular , Mutagénesis Insercional , Mycoplasma gallisepticum/genética , Mycoplasma gallisepticum/metabolismo , Fenilalanina/química , Fenilalanina/genética , Unión Proteica , Conformación Proteica , Estabilidad Proteica , Especificidad de la Especie , Spiroplasma/genética , TemperaturaRESUMEN
Multidose formulations of biotherapeutics, which offer better dosage management and reduced production costs, require the addition of antimicrobial preservatives (APs). APs have been shown, however, to decrease protein stability in solution and cause protein aggregation. In this report, the effect of 4 APs, m-cresol, phenol, phenoxyethanol, and benzyl alcohol on conformational stability, aggregation propensity, and backbone flexibility of an IgG1 mAb, mAb-4, is investigated. Compared with no preservative control, each of the APs decreased the conformational stability of mAb-4 as measured by differential scanning calorimetry and extrinsic fluorescence spectroscopy. The addition of APs resulted in increased monomer loss and aggregate accumulation at 50°C over 28 days, as monitored by size-exclusion chromatography. The extent of conformational destabilization and protein aggregation of mAb-4 induced by APs followed their calculated octanol-water partition coefficients. Increases in backbone flexibility, as measured by hydrogen exchange, of a region located in the CH2 domain of the mAb (heavy chain 237-254) in the presence of APs also correlated with hydrophobicity. Based on these results, the destabilizing effect of APs on mAb-4 correlates with the increased hydrophobicity of the APs and their ability to enhance the local backbone flexibility of an aggregation hot spot within the CH2 domain of the mAb.
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Antiinfecciosos/química , Anticuerpos Monoclonales/química , Inmunoglobulina G/química , Conservadores Farmacéuticos/química , Animales , Alcohol Bencilo/química , Cresoles/química , Glicoles de Etileno/química , Humanos , Modelos Moleculares , Fenol/química , Agregado de Proteínas/efectos de los fármacos , Conformación Proteica/efectos de los fármacos , Estabilidad Proteica/efectos de los fármacosRESUMEN
The transient receptor potential (TRP) superfamily is subdivided into several subfamilies on the basis of sequence similarity, which is highly heterogeneous but shows a molecular architecture that resembles the one present in members of the Kv channel superfamily. Because of this diversity, they produce a large variety of channels with different gating and permeability properties. Elucidation of these particular features necessarily requires comparative studies based on structural and functional data. The present study aims to compilate, analyze, and determine, in a coherent way, the relationship between intrinsic side-chain flexibility and the allosteric coupling in members of the TRPV, TRPM, and TRPC families. Based on the recently determined structures of TRPV1 and TRPV2, we have generated protein models for single subunits of TRPV5, TRPM8, and TRPC5 channels. With these models, we focused our attention on the apparently crucial role of the GP dipeptide at the center of the S4-S5 linker and discussed its role in the interaction with the TRP domain, specifically with the highly-conserved Trp during this coupling. Our analysis suggests an important role of the S4-S5L flexibility in the thermosensitivity, where heat-activated channels possess rigid S4-S5 linkers, whereas cold-activated channels have flexible ones. Finally, we also present evidence of the key interaction between the conserved Trp residue of the TRP box and of several residues in the S4-S5L, importantly the central Pro. Proteins 2017; 85:630-646. © 2016 Wiley Periodicals, Inc.
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Dipéptidos/química , Canales Catiónicos TRPC/química , Canales Catiónicos TRPM/química , Canales Catiónicos TRPV/química , Triptófano/química , Secuencia de Aminoácidos , Animales , Sitios de Unión , Glicina/química , Activación del Canal Iónico , Cinética , Ratones , Modelos Moleculares , Prolina/química , Unión Proteica , Conformación Proteica en Hélice alfa , Dominios y Motivos de Interacción de Proteínas , Ratas , Alineación de Secuencia , Homología de Secuencia de Aminoácido , TermodinámicaRESUMEN
The structural analysis of viruses is often a complex task. In many cases, the details of the viral architecture, especially for enveloped viruses, are limited to low-resolution techniques such as electron microscopy. These structural proteins and assemblies of viruses often populate multiple conformational states and undergo dramatic structural changes, making them difficult to study by most structural methods. They also frequently include highly dynamic regions that are of key functional importance. Many viruses present large surface glycoproteins, which have also proved to be challenging for structural biology due to the intrinsic flexibility and heterogeneity of the glycan decorations. Over the past two decades, hydrogen deuterium exchange coupled to mass spectrometry (HDX-MS) has provided a wealth of information on many diverse viral proteins, glycoproteins, and complexes, in many cases, in multiple conformational states. Here, we describe the methodology for using HDX-MS to investigate the rich structural dynamics of viral systems, and we briefly review the type of systems that have been examined through this type of approach. Though the technique is relatively simple, several potential pitfalls exist at both the sample preparation and the data analysis stage that investigators should be aware of for obtaining reliable data.
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Medición de Intercambio de Deuterio/métodos , Marcaje Isotópico/métodos , Proteínas Virales/química , Virus/química , Secuencia de Aminoácidos/genética , Espectrometría de Masas , Conformación Proteica , Proteínas Virales/aislamiento & purificación , Virus/aislamiento & purificaciónRESUMEN
Protein mobility is important to achieve protein function. Intrinsic flexibility associated with motion underlies this important issue and the analysis of side chain flexibility gives insights to understand it. In this work, the S5-P-S6 pore modulus (PM) of members of Kv and HCN channels was examined by a combination of sequence alignment, residue composition analysis, and intrinsic side chain flexibility. The PM sequences were organized as a database that was used to reveal and correlate the functional diversity of each analyzed family. Specifically, we focused our attention on the crucial role of the S4-S5 linker and its well-described interaction with the S6 T during the electromechanical coupling. Our analysis suggests the presence of a Gly-hinge in the middle of the S4-S5 linkers. This apparent Gly-hinge links a flexible N-terminal segment with a rigid C-terminal one, although in Kv7 channels, the latter segment is even more flexible. Instead, HCN channels exhibit a putative Thr-hinge and is rich in aromatic residues, in consequence, their linker is more rigid. Concerning S6, we confirm the presence of the two flexible kinks previously described and we provide the complete segmental flexibility profiles for the different families. Our results are discussed in terms of the relation between residue composition, conservation, and local conformational flexibility. This provides important insights to understand and differentiate the characteristic gating properties of these channels as well as their implications in cell physiology.