Relevance of pre-stimulus oscillatory activity for the perceived valence of emotional facial expressions.

The interpretation of emotional facial expressions is crucial in everyday social interactions, and rapid processing of these expressions is necessary. Although extensive research has shed light on the mechanisms involved in facial expression processing, there is limited research on the potential role of the state of neural activity that directly precedes the occurrence of a face. Here, we investigated the potential modulatory role of pre-stimulus oscillatory activity in emotional facial expression processing. We tested emotional facial processing in two experiments, one utilizing artificial and the other natural facial expressions. The participants had to evaluate the emotional valence of the presented ambiguous facial expressions. In a univariate analysis, differences in the oscillation activity of the later rated valence of the faces were observed in both experiments, and these differences were observed even before the presentation of the facial expressions. Importantly, two different multivariate approaches directly supported the relevance of pre-stimulus oscillatory activity by exclusively using pre-stimulus oscillatory data to predict the perceived valence of the latter rated facial expression across the two experiments within as well as across subjects. The behavioral data shows the often observed negativity bias, i.e. ambiguous faces resulted in the tendency to rate them as negative. This negativity bias was related to neural activity modulations in the pre-stimulus period and also within post-stimulus processing related activity. These findings underscore the significance of pre-stimulus oscillatory activity in facial expression processing, indicating a functional role of ongoing neural states that affects the processing of facial expressions and constitute a basis for the well described negativity bias.

Increases in pre-stimulus theta and alpha oscillations precede successful encoding of crossmodal associations.

A central aspect of episodic memory is the formation of associations between stimuli from different modalities. Current theoretical approaches assume a functional role of ongoing oscillatory power and phase in the theta band (3-7 Hz) for the encoding of crossmodal associations. Furthermore, ongoing activity in the theta range as well as alpha (8-12 Hz) and low beta activity (13-20 Hz) before the presentation of a stimulus is thought to modulate subsequent cognitive processing, including processes that are related to memory. In this study, we tested the hypothesis that pre-stimulus characteristics of low frequency activity are relevant for the successful formation of crossmodal memory. The experimental design that was used specifically allowed for the investigation of associative memory independent from individual item memory. Participants (n = 51) were required to memorize associations between audiovisual stimulus pairs and distinguish them from newly arranged ones consisting of the same single stimuli in the subsequent recognition task. Our results show significant differences in the state of pre-stimulus theta and alpha power between remembered and not remembered crossmodal associations, clearly relating increased power to successful recognition. These differences were positively correlated with memory performance, suggesting functional relevance for behavioral measures of associative memory. Further analysis revealed similar effects in the low beta frequency ranges, indicating the involvement of different pre-stimulus-related cognitive processes. Phase-based connectivity measures in the theta band did not differ between remembered and not remembered stimulus pairs. The findings support the assumed functional relevance of theta band oscillations for the formation of associative memory and demonstrate that an increase of theta as well as alpha band oscillations in the pre-stimulus period is beneficial for the establishment of crossmodal memory.

Overactivated contextual visual perception and response to a single dose of methylphenidate in children with ADHD.

The pathogenesis of overactivated visual perception in attention-deficit hyperactivity disorder (ADHD) remains unclear, which is interpreted as a cognitive compensation. The existing studies have proposed that perceptual abnormalities in neurodevelopmental disorders are associated with dysfunction of the contextual knowledge system, which influences the development and formation of perception. We hypothesized that alterations in contextual states may also be responsible for inducing perceptual abnormalities in ADHD. Therefore, the present study evaluated the characteristics of pre-stimulus alpha and its response to a single dose of methylphenidate (MPH). A total of 135 Chinese children participated in the first study, including 70 children with ADHD (age = 10.61 ± 1.93 years, female = 17) and 65 age- and sex-matched control children (age = 10.73 ± 1.93 years, female = 20). The second clinical trial included 19 Chinese children with ADHD (age = 11.85 ± 1.72 years, female = 4), with an identical visual spatial search task. Pre-stimulus alpha oscillations and P1 activity were significantly greater in children with ADHD than in the controls. Overactivated pre-stimulus alpha positively predicted P1. Both pre-stimulus alpha and P1 overactivation have beneficial effects on cognitive performance in children with ADHD. No intervening effect of a single dose of MPH on the compensatory activation of pre-stimulus alpha and P1 were observed. Our findings extended the perceptual activation to the contextual knowledge system, suggesting that compensatory perception in children with ADHD is more likely to be a top-down regulated cognitive operational process.

Temporal spectral evolution of pre-stimulus brain activity in visual and visuomotor tasks.

The aim of this study was to describe the spectral features of pre-stimulus event-related potential (ERP) components elicited in visual tasks such as the Bereitschaftspotential (BP), prefrontal negativity (pN) and visual negativity (vN). ERPs are considered time-locked and phase-locked (evoked) activity, but we have also analyzed the non-phase but time-locked (induced) activity in the same interval by applying the temporal spectral evolution (TSE) method. Participants (N = 26) were tested in a passive task, a simple response task (SRT) and a discriminative response task (DRT), where EEG activity was recorded with 64 scalp electrodes. We analyzed the time-frequency modulations (phase and non-phase) prior to the onset of the stimuli in the sub-delta, delta, theta, alpha, beta, and gamma frequency bands. The results showed that all the pre-stimulus ERP components were mainly regulated by evoked activity in the sub-delta band. On the other hand, induced activity seems to be linked to evoked responses but with a different psychophysiological role. We concluded that other preparatory cognitive mechanisms associated with ERPs can also be detected by the TSE method. This finding may suggest underlying mechanisms in non-phase activity and requires the addition of non-phase activity analysis to the traditional analysis (phase and evoked activity).

How the time-of-day affects the EEG signatures of vigilance fluctuation.

Previous research suggested the homeostatic effect on the top-down control system as a major factor for daytime vigilance decrement, yet how it alters the cognitive processes of vigilance remains unclear. Using EEG, the current study measured the vigilance of 28 participants under three states: the morning, the midafternoon after napping and no-nap. The drift-diffusion model was applied to decompose vigilant reaction time into decision and non-decision components. From morning to midafternoon, vigilance declined during sustained wakefulness, but remained stable after midday napping. Increased sleep pressure negatively affected decision time and drift rate, but did not significantly alter the non-decision process. Frontocentral N2 amplitude decreased from morning to no-nap afternoon, associated with slowing decision time. In contrast, parietal P3 had no diurnal alterations during sustained wakefulness, but enhanced after napping. Pre-stimulus parietooccipital alpha power enhanced under high sleep pressure relative to low, accompanied by more lapses in no-nap vs. post-napping conditions. The homeostasis effect is a major contributor to daily vigilance fluctuation, specifically targeting top-down control processes during the pre-stimulus and decision-making stages. Under the influence of sleep homeostasis, the speed of decision-making declines with degradation in target monitoring from morning to afternoon, leading to post-noon vigilance decrement.

Trial-by-trial fluctuations of pre-stimulus alpha power predict language ERPs.

Anticipatory mechanisms are known to play a key role in language, but they have been mostly investigated with violation paradigms, which only consider what happens after predictions have been (dis)confirmed. Relatively few studies focused on the pre-stimulus interval and found that stronger expectations are associated with lower pre-stimulus alpha power. However, alpha power also fluctuates spontaneously, in the absence of experimental manipulations; and in the attention and perception domains, spontaneously low pre-stimulus power is associated with better behavioral performance and with event-related potential (ERPs) with shorter latencies and higher amplitudes. Importantly, little is known about the role of alpha fluctuations in other domains, as it is in language. To this aim, we investigated whether spontaneous fluctuations in pre-stimulus alpha power modulate language-related ERPs in a semantic congruence task. Electrophysiology data were analyzed using Generalized Additive Mixed Models to model nonlinear interactions between pre-stimulus alpha power and EEG amplitude, at the single-trial level. We found that the N400 and the late posterior positivity/P600 were larger in the case of lower pre-stimulus alpha power. Still, while the N400 was observable regardless of the level of pre-stimulus power, a late posterior positivity/P600 effect was only observable for low pre-stimulus alpha power. We discuss these findings in light of the different, albeit connected, functional interpretations of pre-stimulus alpha and the ERPs according to both a nonpredictive interpretation focused on attentional mechanisms and under a predictive processing framework.

Cardiorespiratory fitness modulates prestimulus EEG microstates during a sustained attention task.

Higher cardiorespiratory fitness is associated with an increased ability to perform sustained attention tasks and detect rare and unpredictable signals over prolonged periods. The electrocortical dynamics underlying this relationship were mainly investigated after visual stimulus onset in sustained attention tasks. Prestimulus electrocortical activity supporting differences in sustained attention performance according to the level of cardiorespiratory fitness have yet to be examined. Consequently, this study aimed to investigate EEG microstates 2 seconds before the stimulus onset in 65 healthy individuals aged 18-37, differing in cardiorespiratory fitness, while performing a psychomotor vigilance task. The analyses showed that a lower duration of the microstate A and a higher occurrence of the microstate D correlated with higher cardiorespiratory fitness in the prestimulus periods. In addition, increased global field power and occurrence of microstate A were associated with slower response times in the psychomotor vigilance task, while greater global explained variance, coverage, and occurrence of microstate D were linked to faster response times. Our collective findings showed that individuals with higher cardiorespiratory fitness exhibit typical electrocortical dynamics that allow them to allocate their attentional resources more efficiently when engaged in sustained attention tasks.

Increased neural variability in adolescents with ADHD symptomatology: Evidence from a single-trial EEG study.

Increased intrasubject variability of reaction time (RT) refers to inconsistency in an individual's speed of responding to a task. This increased variability has been suggested as a fundamental feature of attention deficit hyperactivity disorder (ADHD), however, its neural sources are still unclear. In this study, we aimed to examine whether such inconsistency at the behavioral level would be accompanied by inconsistency at the neural level; and whether different types of neural and behavioral variability would be related to ADHD symptomatology. We recorded electroencephalogram (EEG) data from 62 adolescents, who were part of a prospective longitudinal study on the development of ADHD. We examined trial-by-trial neural variability in response to visual stimuli in two cognitive tasks. Adolescents with high ADHD symptomatology exhibited an increased neural variability before the presentation of the stimulus, but when presented with a visual stimulus, this variability decreased to a level that was similar to that exhibited by participants with low ADHD symptomatology. In contrast with our prediction, neural variability was unrelated to the magnitude of behavioral variability. Our findings suggest that adolescents with higher symptoms are characterized by increased neural variability before the stimulation, which might reflect a difficulty in alertness to the forthcoming stimulus; but this increased neural variability does not seem to account for their RT variability.

Pre-stimulus alpha activity modulates long-lasting unconscious feature integration.

Pre-stimulus alpha (α) activity can influence perception of shortly presented, low-contrast stimuli. The underlying mechanisms are often thought to affect perception exactly at the time of presentation. In addition, it is suggested that α cycles determine temporal windows of integration. However, in everyday situations, stimuli are usually presented for periods longer than ∼100 ms and perception is often an integration of information across space and time. Moving objects are just one example. Hence, the question is whether α activity plays a role also in temporal integration, especially when stimuli are integrated over several α cycles. Using electroencephalography (EEG), we investigated the relationship between pre-stimulus brain activity and long-lasting integration in the sequential metacontrast paradigm (SQM), where two opposite vernier offsets, embedded in a stream of lines, are unconsciously integrated into a single percept. We show that increases in α power, even 300 ms before the stimulus, affected the probability of reporting the first offset, shown at the very beginning of the SQM. This effect was mediated by the systematic slowing of the α rhythm that followed the peak in α power. No phase effects were found. Together, our results demonstrate a cascade of neural changes, following spontaneous bursts of α activity and extending beyond a single moment, which influences the sensory representation of visual features for hundreds of milliseconds. Crucially, as feature integration in the SQM occurs before a conscious percept is elicited, this also provides evidence that α activity is linked to mechanisms regulating unconscious processing.

Pre-stimulus gamma power in human posteromedial cortex shows supra-modal mechanisms in predicting the amplitude and latency of task-induced suppression.

Upon repetitively performing the same well-practiced task on identical bottom-up stimuli, our performance still varies. Although it has been well documented that elevated pre-stimulus baseline activity in the human default-mode network impairs the subsequent task performance, it remains unknown (i) the fine-grained temporal dynamics and (ii) whether the underlying neural dynamics are supra-modal or modality-specific. We utilized intracranial recordings in the human posteromedial cortex (PMC) during a simple visual and an auditory detection task. Our findings suggested that the pre-stimulus gamma power in PMC predicted the subsequent task performance. Critically, the higher the pre-stimulus gamma power, the longer it took for it to be suppressed, and the less suppressed it was during the task performance, which eventually resulted in deleterious effects on task performance, i.e. longer reaction times. These fine-grained temporal dynamics were consistent between the visual and auditory simple detection task. In addition, a direct comparison between the visual and auditory modality showed that the between-modality difference emerged during the recovery period from the maximal gamma suppression back to the baseline. Taken together, the present results contribute novel spatio-temporal mechanisms in human PMC on how simple detection performance varies across multiple repetitions, irrespective of the sensory modality involved.

Cortical response variability is driven by local excitability changes with somatotopic organization.

Identical sensory stimuli can lead to different neural responses depending on the instantaneous brain state. Specifically, neural excitability in sensory areas may shape the brain´s response already from earliest cortical processing onwards. However, whether these dynamics affect a given sensory domain as a whole or occur on a spatially local level is largely unknown. We studied this in the somatosensory domain of 38 human participants with EEG, presenting stimuli to the median and tibial nerves alternatingly, and testing the co-variation of initial cortical responses in hand and foot areas, as well as their relation to pre-stimulus oscillatory states. We found that amplitude fluctuations of initial cortical responses to hand and foot stimulation - the N20 and P40 components of the somatosensory evoked potential (SEP), respectively - were not related, indicating local excitability changes in primary sensory regions. In addition, effects of pre-stimulus alpha (8-13 Hz) and beta (18-23 Hz) band amplitude on hand-related responses showed a robust somatotopic organization, thus further strengthening the notion of local excitability fluctuations. However, for foot-related responses, the spatial specificity of pre-stimulus effects was less consistent across frequency bands, with beta appearing to be more foot-specific than alpha. Connectivity analyses in source space suggested this to be due to a somatosensory alpha rhythm that is primarily driven by activity in hand regions while beta frequencies may operate in a more hand-region-independent manner. Altogether, our findings suggest spatially distinct excitability dynamics within the primary somatosensory cortex, yet with the caveat that frequency-specific processes in one sub-region may not readily generalize to other sub-regions.

Pre-stimulus Alpha Activity Modulates Face and Object Processing in the Intra-Parietal Sulcus, a MEG Study.

Face perception is crucial in all social animals. Recent studies have shown that pre-stimulus oscillations of brain activity modulate the perceptual performance of face vs. non-face stimuli, specifically under challenging conditions. However, it is unclear if this effect also occurs during simple tasks, and if so in which brain regions. Here we used magnetoencephalography (MEG) and a 1-back task in which participants decided if the two sequentially presented stimuli were the same or not in each trial. The aim of the study was to explore the effect of pre-stimulus alpha oscillation on the perception of face (human and monkey) and non-face stimuli. Our results showed that pre-stimulus activity in the left occipital face area (OFA) modulated responses in the intra-parietal sulcus (IPS) at around 170 ms after the presentation of human face stimuli. This effect was also found after participants were shown images of motorcycles. In this case, the IPS was modulated by pre-stimulus activity in the right OFA and the right fusiform face area (FFA). We conclude that pre-stimulus modulation of post-stimulus response also occurs during simple tasks and is therefore independent of behavioral responses.

Single-dose testosterone administration modulates instant empathic responses to others' pain: An EEG study.

Whether or not testosterone can impair empathy remains unclear in the literature. Given that empathic responses to others' emotional experiences depend strongly upon top-down controlled mechanisms of attention, here we investigated whether the effects of testosterone administration on pain empathy could be modulated by manipulating attention. We used a double-blind, placebo-controlled within-participant design, in which either testosterone or placebo was administrated in separate sessions. Images depicting painful or nonpainful scenes were presented to induce instant empathic responses. Experiment 1 adopted the pain-judgment and hands-counting tasks to direct attention toward painful or nonpainful aspect of the images, respectively. Experiment 2 employed the pain-rating task to estimate affective and cognitive aspects of pain empathy. When discriminating nonpainful aspects of the images in the hands-counting task, accuracies were lower and empathic late positive potential responses were greater in testosterone sessions than in placebo sessions. This suggested that testosterone enhanced empathic responses to task-irrelevant pain-related features, which interfered with task performance. When providing empathic ratings to the images in the pain-rating task, empathic event-related potentials in the early stage were only observed in the testosterone session. This suggested that testosterone facilitated automatic affective reactivity to others' pain when elaborately processing empathic stimuli. Nevertheless, when discriminating painful aspects of the images in the pain-judgment task, we did not observe any significant differences between the two sessions. These results demonstrated that testosterone effects on enhancing brain reactivity to empathic stimuli were dependent upon task demands deploying attention allocation. The enhancement likely arose from the altered brain state (e.g., increased vigilance and arousal levels) after testosterone administration, as evidenced by the reduced amplitude of spontaneous α-oscillation recorded before the onset of the images. It expands our understanding of the neurobiological mechanisms that affect empathy, and highlights the role of testosterone.

Neural correlates of temporal integration and segregation in metacontrast masking: A phenomenological study.

Temporal integration and segregation have been investigated both in the research on the temporal mechanisms in visual perception and in the research on visual masking. Although both research lines share theoretical, methodological, and empirical similarities, there is little overlap between them and their models of temporal processing are incompatible. As a first step toward the unification of both lines of research, we investigated the electrophysiological correlates of temporal integration and segregation in a metacontrast masking paradigm. Participants reported in each trial whether they perceived the target-mask sequence as a simultaneous or temporally segregated percept while their EEG was recorded. A comparison of both temporal report categories resulted in an ERP difference after stimulus presentation (200-450 ms) that closely resembles the contour integration negativity. Moreover, we found that phase states were shifted between perceptual report categories in the alpha (450-250 ms) and beta (225-125 ms) frequency band before stimulus presentation and induced a sinusoidal periodicity in later temporal report proportions. Thus, we show that neural correlates of temporal integration and segregation can be generalized to metacontrast masking. These findings emphasize the potential role of temporal mechanisms in the emergence of the masking phenomenon. Additionally, our findings validate our phenomenological approach by demonstrating similar neural correlates of temporal integration and segregation as in performance-based tasks. Future research may profit from our phenomenological approach to disentangle the (neural) interplay between temporal and masking mechanisms.

From Shorter to Longer Timescales: Converging Integrated Information Theory (IIT) with the Temporo-Spatial Theory of Consciousness (TTC).

Time is a key element of consciousness as it includes multiple timescales from shorter to longer ones. This is reflected in our experience of various short-term phenomenal contents at discrete points in time as part of an ongoing, more continuous, and long-term 'stream of consciousness'. Can Integrated Information Theory (IIT) account for this multitude of timescales of consciousness? According to the theory, the relevant spatiotemporal scale for consciousness is the one in which the system reaches the maximum cause-effect power; IIT currently predicts that experience occurs on the order of short timescales, namely, between 100 and 300 ms (theta and alpha frequency range). This can well account for the integration of single inputs into a particular phenomenal content. However, such short timescales leave open the temporal relation of specific phenomenal contents to others during the course of the ongoing time, that is, the stream of consciousness. For that purpose, we converge the IIT with the Temporo-spatial Theory of Consciousness (TTC), which, assuming a multitude of different timescales, can take into view the temporal integration of specific phenomenal contents with other phenomenal contents over time. On the neuronal side, this is detailed by considering those neuronal mechanisms driving the non-additive interaction of pre-stimulus activity with the input resulting in stimulus-related activity. Due to their non-additive interaction, the single input is not only integrated with others in the short-term timescales of 100-300 ms (alpha and theta frequencies) (as predicted by IIT) but, at the same time, also virtually expanded in its temporal (and spatial) features; this is related to the longer timescales (delta and slower frequencies) that are carried over from pre-stimulus to stimulus-related activity. Such a non-additive pre-stimulus-input interaction amounts to temporo-spatial expansion as a key mechanism of TTC for the constitution of phenomenal contents including their embedding or nesting within the ongoing temporal dynamic, i.e., the stream of consciousness. In conclusion, we propose converging the short-term integration of inputs postulated in IIT (100-300 ms as in the alpha and theta frequency range) with the longer timescales (in delta and slower frequencies) of temporo-spatial expansion in TTC.

Combined influences of strategy and selection history on attentional control.

Visual attention is guided by top-down mechanisms and pre-stimulus task preparation, but also by selection history (i.e., the bias to prioritize previously attended items). Here we examine how these influences combine. Two groups of participants completed two intermingled tasks. One task involved categorization of a unique target; one group categorized the target based on color, and the other based on shape. The other task involved searching for a target defined by unique shape while ignoring a distractor defined by unique color. Our expectation was that the search task would be difficult for the color-categorization group because their categorization task required attentional resolution of color, but the search task required that they ignore color. In some experimental blocks, trials from the two tasks appeared predictably, giving the color-categorization group an opportunity to strategically prepare by switching between color-prioritizing and shape-prioritizing attentional templates. We looked to pre-stimulus oscillatory activity as a direct index of this preparation, and to reaction times and post-stimulus ERPs for markers of resultant change in attentional deployment. Results showed that preparation in the color-categorization group optimized attentional templates, such that these participants became less sensitive to the color distractor in the search task. But preparation was not sufficient to entirely negate the influence of selection history, and participants in the color-categorization group continued to show a propensity to attend to the color distractor. These results indicate that preparatory effort can be scaled to the anticipated attentional requirements, but attention is nevertheless considerably biased by selection history.

The role of the anterior nuclei of the thalamus in human memory processing.

Extensive neuroanatomical connectivity between the anterior thalamic nuclei (ATN) and hippocampus and neocortex renders them well-placed for a role in memory processing, and animal, lesion, and neuroimaging studies support such a notion. The deep location and small size of the ATN have precluded their real-time electrophysiological investigation during human memory processing. However, ATN electrophysiological recordings from patients receiving electrodes implanted for deep brain stimulation for pharmacoresistant focal epilepsy have enabled high temporal resolution study of ATN activity. Theta frequency synchronization of ATN and neocortical oscillations during successful memory encoding, enhanced phase alignment, and coupling between ATN local gamma frequency activity and frontal neocortical and ATN theta oscillations provide evidence of an active role for the ATN in memory encoding, potentially integrating information from widespread neocortical sources. Greater coupling of a broader gamma frequency range with theta oscillations at rest than during memory encoding provides additional support for the hypothesis that the ATN play a role in selecting local, task-relevant high frequency activity associated with particular features of a memory trace.

Sustained visuospatial attention enhances lateralized anticipatory ERP activity in sensory areas.

The existence of neural correlates of spatial attention is not limited to the reactive stage of stimulus processing: neural activities subtending spatial attention are deployed well ahead of stimulus onset. ERP evidence supporting this proactive (top-down) attentional control is based on trial-by-trial S1-S2 paradigms, where the onset of a directional cue (S1) indicates on which side attention must be directed to respond to an upcoming target stimulus (S2). Crucially, S1 onset trigger both attention and motor preparation, therefore, these paradigms are not ideal to demonstrate the effect of attention at preparatory stage of processing. To isolate top-down anticipatory attention, the present study used a sustained attention paradigm based on a steady cue that indicates the attended side constantly throughout an entire block of trials, without any onset of an attentional cue. The main result consists in the description of the attention effect on the visual negativity (vN) component, a growing neural activity starting before stimulus presentation in extrastriate visual areas. The vN was consistently lateralized in the hemisphere contralateral to the attended side, regardless of the hand to be used. At the opposite, the lateralized motor activity emerged long after, confirming that the hand-selection process followed the spatial attention orientation process. The present study confirms the anticipatory nature of the vN component and corroborate its role in terms of preparatory visuospatial attention.

Acoustic pre-stimulation modulates startle and postural reactions during sudden release of standing support surface in aging.

Falls contribute to injuries and reduced level of physical activity in older adults. During falls, the abrupt sensation of moving downward triggers a startle-like reaction that may interfere with protective response movements necessary to maintain balance. Startle reaction could be dampened by sensory pre-stimulation delivered immediately before a startling stimulus. This study investigated the neuromodulatory effects of pre-stimulation on postural/startle responses to drop perturbations of the standing support surface in relation to age. Ten younger and 10 older adults stood quietly on an elevated computer-controlled moveable platform. At an unpredictable time, participants were dropped vertically to elicit a startle-like response. Reactive drop perturbation trials without a pre-stimulus (control) were alternated with trials with acoustic pre-stimulus tone (PSI). A two-way mixed design analysis of variance comparing condition (control vs. PSI) X group (younger vs. older) was performed to analyze changes in muscle activation patterns, ground reaction force, and joint angular displacements. Compared to younger adults, older adults showed lower neck muscle electromyography amplitude reduction rate and incidence of response. Peak muscle activation in neck, upper arm, and hamstring muscles were reduced during PSI trials compared to control trials in both groups (p < 0.05). In addition, knee and hip joint flexion prior to ground contact was reduced in PSI trials compared to control (p < 0.05). During post-landing balance recovery, increased knee and hip flexion displacement and time to peak impact force were observed in PSI trials compared to control condition (p < 0.05). PSI reduced startle-induced muscle activation at proximal body segments and likely decreased joint flexion during abrupt downward vertical displacement perturbations of the body. Older adults retained the ability to modulate startle and postural responses but their neuromodulatory capacity was reduced compared with younger adults. Further research on the potential of applying PSI as a possible therapeutic tool to reduce the risk of fall-related injury is needed.