Analysis of Ca1-xSrxCO3 phases generated by competitive Sr2+ replacement in pre-formed aragonite.

The ratio of Sr/Ca ions in marine biogenic minerals is considered advantageous for tracking geochemical and biomineralization processes that occur in the oceans. It is debatable, though, whether the ratio in biominerals such as coral skeleton is simply related to values in the seawater environment or controlled by the organism. Recent data show that coral larvae produce partially disordered immature aragonite in Mg-containing Sr-poor calcifying fluids, which transforms into well-ordered aragonite in Mg-depleted Sr-enriched environments, upon animal metamorphosis into the sessile polyp state. Inspired by the process in young coral, we explored in vitro substitution of Ca by Sr in aragonite by exposing aragonite crystals precipitated a priori to Sr solutions with variable concentrations. The resulting biphasic material, comprised of Sr-doped aragonite and Ca-doped strontianite, was carefully analyzed for foreign cation substitution in each polymorph. This allowed to establish a linear correlation between Sr levels in mineralizing solutions and Sr in aragonite as well as Ca in strontianite. It indicated that ca. 5-fold higher Sr solution concentration is needed for substitution in the crystal to reach the level found in corals. It also provided with Sr levels required for a putative strontianite phase to form.

Absolute Configuration of the Invasive Mealybug Delottococcus aberiae (De Lotto) Sex Pheromone: Synthesis and Bioassay of Both Enantiomers.

The mealybug Delottococcus aberiae (De Lotto) (Hemiptera: Pseudococcidae) is an invasive pest reported in Europe at the end of the first decade of the 2000s, causing severe damage to citrus production in eastern Spain. In a previous work, (4,5,5-trimethyl-3-methylenecyclopent-1-en-1-yl)methyl acetate was identified as the sex pheromone emitted by females, a new compound with an unusual ß-necrodol skeleton possessing one stereocenter. This compound was assigned to the (-)-enantiomer but the absolute configuration was then not reported. In the present study, enantiomeric pure samples of both enantiomers were synthesized. X-ray diffraction analysis allowed the (-)-enantiomer, identical to the one emitted by virgin D. aberiae females, to be unequivocally identified as (-)-(R)-(4,5,5-trimethyl-3-methylenecyclopent-1-en-1-yl)methyl acetate. Bioassays carried out to test the activity of both enantiomers under field conditions suggest that the presence of the (+)-(S)-enantiomer has detrimental effects on the activity of the racemates.

Nitrogen plasma-induced phase engineering and titanium carbide/carbon nanotubes dual conductive skeletons endow molybdenum disulfide with significantly improved lithium storage performance.

MoS2/Ti3C2 MXene composite has emerged as a promising anode material for lithium storage due to the synergistic combination of high specific capacity offered by MoS2 and conductive skeleton provided by Ti3C2 MXene. However, its two-dimensional/two-dimensional (2D/2D) structure is susceptible to collapse after long cycles, while the inherent low conductivity of MoS2 limits its rate performance. In this study, we developed a novel approach combining plasma-induced phase engineering with dual skeleton structure design to fabricate a unique P-MoS2/Ti3C2/CNTs anode material featuring highly conductive 1T phase MoS2 and a stable one-dimensional/two-dimensional (1D/2D) architecture. Within this architecture, growth of MoS2 nanosheets on the surface of Ti3C2 cross-linked by carbon nanotubes (CNTs) was achieved. The resulting Ti3C2/CNTs dual skeleton not only provides robust mechanical support to prevent structural collapse during long cycles but also offers increased specific surface area and additional Li+ storage space, thereby enhancing the lithium storage capacity of the composite. Subsequent N2 plasma treatment induced a phase transition in MoS2 from 2H to 1T configuration. Density functional theory (DFT) calculations confirmed that the induced 1T-MoS2 exhibits higher conductivity and lower Li+ diffusion barrier compared to 2H-MoS2. Benefiting from these synergistic effects, our P-MoS2/Ti3C2/CNTs anode demonstrated remarkable electrochemical performance including a high reversible specific capacity of 1120 mAh g-1 at 0.1 A g-1, excellent cycling stability with a specific capacity retention of 670 mAh g-1 after 600 cycles at 1 A/g, and superior rate performance with a specific capacity of 614 mAh g-1 at 2 A g-1. This combined modification strategy will serve as guidance for designing other energy storage materials.

A review of recent advances in metal-organic frameworks materials for zero-energy passive adsorption of chemical pollutants in indoor environments.

Approximately 75-90 % of a person's lifetime is spent inside increasingly airtight buildings, where indoor pollutant levels typically exceed those outdoors. Poor indoor air quality can lead to allergies, respiratory diseases, and even cancer, and can also reduce the longevity of buildings. Passive adsorption materials play a crucial role in reducing indoor pollutants. This review highlights the latest advances in using Metal-organic Frameworks (MOFs) as passive adsorption materials for indoor pollutant capture and outlines the principles for developing high-performance adsorbents. It provides a comparative analysis of the development and performance of MOFs and composite adsorbent materials, highlighting their respective advantages and limitations in indoor pollutant adsorption technology. The article proposes strategies to address these challenges and offers a comprehensive review of current practical adsorption devices. Finally, aiming to advance commercialization of MOFs, the anticipated development of indoor pollutant adsorption technology is discussed in this paper.

The destruction of cytoplasmic skeleton leads to the change of nuclear structure and the looseness of lamin A submicroscopic network.

The interaction between lamin A and the cytoplasmic skeleton plays a key role in maintaining nuclear mechanical properties. However, the effect of destruction of the cytoplasmic skeleton on the 3D submicroscopic structure of lamin A has not been elucidated. In this study, we developed an image quantization algorithm to quantify changes in the submicroscopic structure of the intact lamin A 3D network within the nucleus. We used blebbistatin or nocodazole to disrupt the fibrillar structure of F-actin or tubulin, respectively, and then quantified changes in the lamin A super-resolution network structure, the morphological and mechanical properties of the nucleus and the spatial distribution of chromosomes. Ultimately, we found for the first time that disruption of the cytoplasmic skeleton changes the lamin A submicroscopic network and nuclear structural characteristics. In summary, this study contributes to understanding the trans-nuclear membrane interaction characteristics of lamin A and the cytoplasmic skeleton.

A 3D micro-computed tomography study comparing embryonic skeletal development in layer versus broiler strains of the domestic chicken.

Our objective was to analyze the effect of selection for divergent traits in the domestic chicken on embryonic skeletal development, which could affect postnatal bird welfare. Development was compared between the Ross 308 broiler line (fast growth and muscle mass accrual) and Novoponte layers (high laying rate and egg quality). In Study 1 (Initial Conditions), we characterized egg composition prior to incubation and identified the onset of embryonic skeletal mineralization in the 2 strains. In Study 2 (Developmental Dynamics), we used 3D X-ray tomographic imaging (µCT) on incubation days ED11, ED13, ED15 and ED17 to track skeletal maturation trajectories as a pseudo-time series. Results showed that Ross 308 embryos, which are heavier than Novoponte embryos, possess higher levels of yolk nutrients including phosphorus and calcium, but lower eggshell mineral content, than Novoponte embryos. Skeletal mineralization started synchronously in both strains, on ED11. The higher mineral ion content in the larger yolk of Ross 308 eggs compared to Novoponte eggs may partly explain why skeletal mineralization in Ross 308 embryos advances faster: using µCT, we show that the mandible and tibiotarsi in Ross 308 embryos are larger at ED11 and ED13 compared with Novoponte embryos. However, Novoponte embryos catch up from this initial lag in mineralization by ED15. The timing of the Novoponte acceleration coincides with the functional activation of the chorioallantoic membrane in releasing calcium from the inner eggshell. This correlates with a decrease in eggshell thickness from ED11 to ED17 in Novoponte eggs, which was not observed during Ross 308 incubation. To conclude, while some temporal discrepancies exist in early skeletal development between the embryos of Ross 308 and Novoponte strains, overall prenatal skeletal maturation seems to be robustly regulated. Despite selection for antagonist production traits, layer and broiler prehatch skeletal morphology ultimately synchronizes. Practically, since the extent of skeletal maturity equalizes between strains towards the end of incubation, refinements of farming practices, postnatal environment, and diet should be considered for improving domestic fowl welfare.

Osteology of the Small-Sized Hyphessobrycon piabinhas Fowler 1941 (Characiformes, Characidae) With a Discussion on Developmentally Truncated Characters in the Family.

Evolutionary body size decrease has profound consequences for the morphology of an organism. In the evolution of the Characidae, the most species-rich family of Neotropical fishes, a prominent trend is the reduction of body size. The most typical effect is the simplification and reduction of morphological features through terminal deletion processes, resulting in the loss of skeletal elements and structures. To provide further information on the matter, we present a detailed description of the skeleton of Hyphessobrycon piabinhas, a poorly known, small representative of the largest genus of Characidae. We further discuss the identity and phylogenetic relationships of H. piabinhas. It belongs to the subfamily Stethaprioninae and exhibits considerable morphological similarity to other congeners from neighboring drainage systems. We identify several morphological simplifications in H. piabinhas and discuss them based on ontogenetic data available for Characiformes. These developmentally truncated elements are also present in many other small representatives of the family and seem to be among the first morphological modifications to occur in the context of body size reduction of Characidae. We argue that structural losses are not strictly correlated with sizes below 26 mm SL, although the most notable simplifications are typically observed in the miniatures.

Fabrication of Helical Carbon Fiber Skeleton Using Arc Glow Discharge Method.

An arc glow discharge device was used to prepare a helical carbon fiber skeleton with helical carbon fibers hooked to each other by spraying a hydrogen and ethanol mixture onto the iron wire substrate through the discharge area, using anhydrous ethanol as the carbon source. The samples were characterized by SEM, EDS, Raman and XPS. A growth mechanism of helical carbon fiber driven by C sp3 was proposed. The various growth modes of carbon fiber during the formation of carbon fiber skeleton were investigated. A ring appearance that indicated a change in the direction of carbon fiber growth was observed. And double helical carbon fiber was constructed from single helical carbon fiber in two ways. Super-large carbon fiber with a diameter of about 13 µm was observed, and it was speculated that this super-large carbon fiber is the backbone of the carbon fiber skeleton. The mechanical properties of the carbon fiber skeleton are isotropic.

An Experimental Study on the Thermal Performance of a Heat Sink Filled with Porous Aluminum Skeleton/Paraffin Composite Phase Change Material.

Organic phase change material is an ideal solution to solve the heat dissipation problem of electronic devices. However, its low thermal conductivity limits its application. To solve this problem, a new porous aluminum skeleton/paraffin composite phase change material (AS-PCM) was prepared. The effects of porosity and porous aluminum skeletons on temperature control performance were explored. The experimental results show that the addition of AS significantly improves the thermal conductivity of organic PCM, and the thermal conductivity of AS-PCM is 32.3-59.6 times higher than that of pure paraffin. In addition, the temperature difference in AS-PCM with 75% porosity is 1-2 °C lower than that of AS-PCM with 85%, and 5-8 °C lower than that of AS-PCM with 95% porosity. The skeleton structure has an impact on the temperature control performance. The Mcc porous aluminum skeleton/paraffin composite phase change material (MAS-PCM) yields the best thermal performance compared with the Fcc porous aluminum skeleton/paraffin composite phase change material (FAS-PCM). The temperature control time of the MAS-PCM heat sink is increased by 5.3-50.8% relative to the FAS-PCM heat sink. The research results provide a novel approach for improving the thermal conductivity of PCMs.

Usage, Attitudes, Facilitators, and Barriers Toward Digital Health Technologies in Musculoskeletal Care: Survey Among Primary Care Physiotherapists in Norway.

BACKGROUND: Work burden increases for physiotherapists in the primary health care sector as the prevalence of musculoskeletal disorders (MSDs) increases. Digital health technologies (DHTs) are proposed as a viable solution to secure the sustainability of the health care system and have shown promising results in a range of conditions. However, little is known about use of DHTs among physiotherapists in the primary health care sector in Norway. OBJECTIVE: This study aimed to investigate the use of and attitudes toward DHTs among physiotherapists treating patients with MSDs in primary care, and potential facilitators or barriers for adopting DHTs in clinical practice. METHODS: An author-developed web-based questionnaire was distributed to physiotherapists in all Norwegian municipalities in March 2023. The questionnaire included items regarding use of technologies, attitudes, suitability, and factors influencing adoption of DHT. Suitability and agreement on statements were scored on an 11-point numeric rating scale (0=very unsuitable or strongly disagree, 10=very suitable or strongly agree). Differences across employment sites and users versus nonusers of DHT were analyzed using the χ2 test, Fisher exact test, Student t test, and Mann-Whitney U test. RESULTS: Approximately 5000 physiotherapists were invited to participate, of which 6.8% (338) completed the questionnaire. A total of 46.2% (156/338) offered DHTs in their practice, of which 53.2% (83/156) used it on a weekly basis, mostly telephone consultations (105/156, 67.3%). A higher proportion of physiotherapists in private practice offered DHT compared with those employed by municipalities (95/170, 55.9% vs 61/168, 36.3%; P<.001). A majority (272/335, 81.2%) were positive about recommending DHTs to their patients. Suitability of DHTs in physiotherapy was rated an average of 6 (SD 2.1). Apps for smartphones or tablets were rated most suitable (mean rating 6.8, SD 2.4). The most frequently reported advantages were flexibility in how physiotherapy is offered (278/338, 82.3%) and reduced travel time for the patient (235/338, 70%). The highest rated disadvantages were limited scope for physical examination (252/338, 74.6%) and difficulty in building rapport with the patient (227/338, 67.2%). The main facilitators and barriers included a functioning (median rating 10, IQR 8-10) or lack of functioning (median rating 9, IQR 8-10) internet connection, respectively. Lack of training in DHTs was prominent regarding evaluation, diagnosing, and treatment (median rating 0, IQR 0-2), with minor, but significant, differences between nonusers and users (median rating 0, IQR 0-1 vs median rating 1, IQR 0-4); P<.001). CONCLUSIONS: Physiotherapists in Norwegian primary care treating patients with MSDs are positive about using DHTs, and almost 50% (156/338) have adopted them in clinical practice. Concerns are related to lack of a physical examination and technical aspects. Training in the use of DHTs should be addressed in implementation processes.

Role of bisphosphonates in osteoporosis caused by adult growth hormone deficiency. / åŒè†¦é ¸ç›åœ¨æˆäººç”Ÿé•¿æ¿€ç´ ç¼ºä¹æ‰€è‡´éª¨è´¨ç–æ¾ç—‡ä¸­çš„ä½œç”¨ï¼ˆè‹±æ–‡ï¼‰.

In recent years, growth hormone and insulin-like growth factors have become key regulators of bone metabolism and remodeling, crucial for maintaining healthy bone mass throughout life. Studies have shown that adult growth hormone deficiency leads to alterations in bone remodeling, significantly affecting bone microarchitecture and increasing fracture risk. Although recombinant human growth hormone replacement therapy can mitigate these adverse effects, improving bone density, and reduce fracture risk, its effectiveness in treating osteoporosis, especially in adults with established growth hormone deficiency, seems limited. Bisphosphonates inhibit bone resorption by targeting farnesyl pyrophosphate synthase in osteoclasts, and clinical trials have confirmed their efficacy in improving osteoporosis. Therefore, for adult growth hormone deficiency patients with osteoporosis, the use of bisphosphonates alongside growth hormone replacement therapy is recommended.

Correlation of spheno-occipital synchondrosis and mandibular condylar cortication with chronological age using computed tomography in Indian population- A cross-sectional study.

BACKGROUND: Forensic age estimation is a procedure which utilises many methods to estimate the age of both living and deceased individuals, including those who have died in natural disasters or man-made catastrophes. The pattern and closure of spheno-occipital synchondrosis (SOS) fusion, along with subchondral ossification of the mandibular condyle, can be used to estimate age. AIM AND OBJECTIVES: This study aims to estimate age using computed tomographic (CT) images of spheno-occipital synchondrosis fusion (SOS) and mandibular condylar cortication (MCC), and to correlate these findings with chronological age. MATERIALS AND METHODS: The present study included 435 CT images of individuals aged 10-25 years. SOS fusion was assessed using a four-stage system, and MCC was assessed bilaterally using a three-stage system on the sagittal plane. Data on fusion stages and cortication types were entered along with chronological age, and then statistically analysed. RESULTS: SOS fusion stage 2 occurred at similar age in males (19.82 ± 2.67 years) and females (19.23 ± 2.93 years). Earlier fusion of other stages was observed in females by a mean age of 2 years. MCC was completed 1 year earlier in females, with statistically significant differences (p ≤ 0.001). When comparing cortication types and different fusion stages, only type II cortication showed statistically significant differences compared to different fusion stages (p ≤ 0.001). CONCLUSION: Mandibular condylar cortication (MCC) and spheno-occipital synchondrosis (SOS) fusion were positively correlated with chronological age, suggesting that these parameters can be used as an adjunct method for age estimation.

Terpene extract from the stem of Celastrus orbiculatus inhibits actin cytoskeleton remodelling in gastric cancer cells by regulating the protein interaction between PTBP1 and ACTN4.

Adjuvant chemoradiotherapy, molecular targeted therapy, and immunotherapy are frequently employed to extend the survival of patients with advanced gastric cancer (GC). However, most of these treatments have toxic side effects, drug resistance, and limited improvements in survival and quality of life. Therefore, it is crucial to discover and develop new medications targeting GC that are highly effective and have minimal toxicity. In previous studies, the total terpene extract from the stem of Celastrus orbiculatus demonstrated anti-GC activity; however, the specific mechanism was unclear. Our research utilising co-immunoprecipitation-mass spectrometry (Co-IP-MS), polypyrimidine tract binding protein 1 (ptbp1) clustered regularly interspaced short palindromic repeat-associated protein 9 (Cas9)-knockout (KO) mouse model, tissue microarray, and functional experiments suggests that alpha actinin-4 (ACTN4) could be a significant biomarker of GC. PTBP1 influences actin cytoskeleton restructuring in GC cells by interacting with ACTN4. Celastrus orbiculatus stem extract (COE) may directly target ACTN4 and affect the interaction between PTBP1 and ACTN4, thereby exerting anti-GC effects.

Skeletal microstructures of cheilostome bryozoans (phylum Bryozoa, class Gymnolaemata): crystallography and secretion patterns.

Gymnolaemata bryozoans produce CaCO3 skeletons of either calcite, aragonite, or both. Despite extensive research, their crystallography and biomineralization patterns remain unclear. We present a detailed study of the microstructures, mineralogy, and crystallography of eight extant cheilostome species using scanning electron microscopy, electron backscatter diffraction, atomic force microscopy, and micro-computed tomography. We distinguished five basic microstructures, three calcitic (tabular, irregularly platy, and granular), and two aragonitic (granular-platy and fibrous). The calcitic microstructures consist of crystal aggregates that transition from tabular or irregularly platy to granular assemblies. Fibrous aragonite consists of fibers arranged into spherulites. In all cases, the crystallographic textures are axial, and stronger in aragonite than in calcite, with the c-axis as the fiber axis. We reconstruct the biomineralization sequence in the different species by considering the distribution and morphology of the growth fronts of crystals and the location of the secretory epithelium. In bimineralic species, calcite formation always predates aragonite formation. In interior compound walls, growth proceeds from the cuticle toward the zooecium interior. We conclude that, with the exception of tabular calcite, biomineralization is remote and occurs within a relatively wide extrapallial space, which is consistent with the inorganic-like appearance of the microstructures. This biomineralization mode is rare among invertebrates. Supplementary Information: The online version contains supplementary material available at 10.1007/s42995-024-00233-1.

Skeletal muscle activity affects the deformity of long bone morphology in lathyritic chick embryo.

Embryonic muscle activity is involved in various aspects of bone morphogenesis and growth. Normal mechanical stimuli of muscle contraction are important in most cases, and when the muscles are immobilized, the developing bones are abnormally shaped. In chick embryos, a characteristic curved deformity is reproducibly induced in the developing tibiotarsus using the bone-weakening agent, beta-aminopropionitrile (bAPN). In this study, we applied decamethonium bromide (DMB), a well-established neuromuscular blocking agent, to embryos treated with bAPN, to test the hypothesis that the deformity is triggered and formed depending on the balance between the decrease in stiffness of the bAPN-affected tibiotarsus and the normal physiological increase in embryonic skeletal muscle activity. The occurrence of curved morphology induced by bAPN administered at 4 or 8 days of incubation (embryonic day [ED]) was temporally consistent with the posterior displacement of the leg muscles, which occurred just before ED8. The displaced muscles were assumed to exert a contraction force comparable to that of untreated normal muscles. When treated with DMB at ED8, the muscles atrophied and exhibited degenerative changes, and the degree of curved morphology was alleviated and reduced to 50% or more in the morphometric evaluation at ED10. These findings indicated that the coordinated development of skeletal element stiffness and muscle activity must be temporally regulated, particularly during the early stages of skeletogenesis.

Synthesis of Tumor Selective Indole and 8-Hydroxyquinoline Skeleton Containing Di-, or Triarylmethanes with Improved Cytotoxic Activity.

The reaction between glycine-type aminonaphthol derivatives substituted with 2- or 1-naphthol and indole or 7-azaindole has been tested. Starting from 2-naphthol as a precursor, the reaction led to the formation of ring-closed products, while in the case of a 1-naphthol-type precursor, the desired biaryl ester was isolated. The synthesis of a bifunctional precursor starting from 5-chloro-8-hydroxyquinoline, morpholine, and ethyl glyoxylate via modified Mannich reaction is reported. The formed Mannich base 10 was subjected to give bioconjugates with indole and 7-azaindole. The effect of the aldehyde component and the amine part of the Mannich base on the synthetic pathway was also investigated. In favor of having a preliminary overview of the structure-activity relationships, the derivatives have been tested on cancer and normal cell lines. In the case of bioconjugate 16, as the most powerful scaffold in the series bearing indole and a 5-chloro-8-hydroxyquinoline skeleton, a potent toxic activity against the resistant Colo320 colon adenocarcinoma cell line was observed. Furthermore, this derivative was selective towards cancer cell lines showing no toxicity on non-tumor fibroblast cells.

Ecotoxicological risk assessment of the novel psychoactive substance Esketamine: Emphasis on fish skeletal, behavioral, and vascular development.

Novel psychoactive substances (NPS), such as Esketamine (Esket), often contaminate the aquatic ecosystems following human consumption, raising concerns about the residues and potential ecological hazards to non-target organisms. The study used zebrafish as a model organism to investigate the developmental toxicity and ecotoxicological effects of acute Esket exposure. Our findings demonstrate that exposure to Esket significantly affected the early development and angiogenesis of zebrafish embryos/larvae. The mandible length was significantly decreased, and the angles between the pharyngeal arch cartilages were narrowed compared to the control group (all P < 0.05). Additionally, Esket resulted in a decrease of 47.6-89.8 % in the number of neural crest cells (NCC). Transcriptome analysis indicated altered expression of genes associated with cartilage and osteoblast growth. Moreover, Esket significantly inhibited swimming ability in zebrafish larvae and was accompanied by behavioral abnormalities and molecular alterations in the brain. Potential mechanisms underlying Esket-induced behavioral disorders involve neurotransmitter system impairment, abnormal cartilage development and function, aberrant vascular development, as well as perturbations in oxidative stress and apoptosis signaling pathways. Notably, the dysregulation of skeleton development through the bone morphogenetic protein (BMP) signaling pathway is identified as the primary mechanistic behind Esket-induced behavioral disorder. This study enhances our understanding of Esket's ecotoxicology profile and provides a comprehensive assessment of the environmental risks associated with NPS.

Enhancement of alkaline pretreatment-anaerobically digested sludge dewaterability by chitosan and rice husk powder for land use of biogas slurry.

Alkaline pretreatment can improve the methane yields and dewatering performance of anaerobically digested sludge, but it still needs to be coupled with other conditioning methods in the practical dewatering process. This study utilized four different flocculants and a skeleton builder for conditioning of alkaline pretreatment-anaerobically digested sludge. Chitosan was found to be the most effective in dewatering the sludge. Chitosan coupled with rice husk powder further improved the dewatering performance, which reduced normalized capillary suction time, specific resistance to filtration, and moisture content by 98.7%, 82.0%, and 12.1%. For land use of biogas slurry as a fertilizer, chitosan conditioning promoted the growth of corn seedlings, while the other three flocculants diminished the growth of corn seedlings. Chitosan coupled with rice husk powder further promoted the growth of corn seedlings by 103.5%, 65.0%, and 53.7% in fresh weight, dry weight, and root length, respectively. Overall, chitosan coupled with rice husk powder not only enhanced the dewaterability of alkaline pretreatment-anaerobically digested sludge but also realized the resource utilization of agricultural waste.

Hydrocortisone treatment as a tool to study conjunctival placode induction.

BACKGROUND: Conjunctival placodes are a series of placodes that develop into the conjunctival (scleral) papillae and ultimately induce a series of scleral ossicles in the eyes of many vertebrates. This study establishes a hydrocortisone injection procedure (incl. dosage) that consistently inhibits all conjunctival papillae in the embryonic chicken eye. The effects of this hydrocortisone treatment on apoptosis, vasculature, and placode-related gene expression were assessed. RESULTS: Hydrocortisone treatment does not increase apoptotic cell death or have a major effect on the ciliary artery or vascular plexus in the eye. ß-catenin and Eda expression levels were not significantly altered following hydrocortisone treatment, despite the absence of conjunctival papillae. Notably, Fgf20 expression was significantly reduced following hydrocortisone treatment, and the distribution of ß-catenin was altered. CONCLUSIONS: Our study showed that conjunctival papillae induction begins as early as HH27.5 (E5.5). Hydrocortisone treatment reduces Fgf20 expression independently of ß-catenin and Eda and may instead affect other members of the Wnt/ß-catenin or Eda/Edar pathways, or it may affect the ability of morphogens to diffuse through the extracellular matrix. This study contributes to a growing profile of gene expression data during placode development and enhances our understanding of how some vertebrate eyes develop these fascinating bones.

Near-Infrared Forearm Vascular Width Calculation Using Radius Estimation of Tangent Circle.

In response to the analysis of the functional status of forearm blood vessels, this paper fully considers the orientation of the vascular skeleton and the geometric characteristics of blood vessels and proposes a blood vessel width calculation algorithm based on the radius estimation of the tangent circle (RETC) in forearm near-infrared images. First, the initial infrared image obtained by the infrared camera is preprocessed by image cropping, contrast stretching, denoising, enhancement, and initial segmentation. Second, the Zhang-Suen refinement algorithm is used to extract the vascular skeleton. Third, the Canny edge detection method is used to perform vascular edge detection. Finally, a RETC algorithm is developed to calculate the vessel width. This paper evaluates the accuracy of the proposed RETC algorithm, and experimental results show that the mean absolute error between the vessel width obtained by our algorithm and the reference vessel width is as low as 0.36, with a variance of only 0.10, which can be significantly reduced compared to traditional calculation measurements.