Forage sources in total mixed rations early in life influence performance, metabolites, and behavior of dairy calves.

The objective of this study was to evaluate effects of forage inclusion and sources on performance, metabolism, and feeding behavior of dairy calves. Forty-eight Holstein calves were blocked and randomly assigned to 1 of 4 dietary treatments according to sex and BW at 28 d of life to determine the effects of feeding forage sources (ensiled and dry), with different quality on performance, metabolites, and behavior. Treatments consisted of a no-forage coarsely ground starter (CON); or total mixed ration (TMR) containing 7.5% on a dry matter (DM) basis of Tifton hay of either medium quality (MH) or low quality (LH); or 10% on a DM basis of corn silage (CS). During the first 28 d of life, all calves received 3 L of whole milk twice daily, a commercial pelleted starter and no forage, and water ad libitum. After that, the solid diet was changed to the respective dietary treatments. Calves were gradually weaned from 52 to 56 d of age, and followed for 14 d postweaning. Individual solid feed and milk intakes were recorded daily, and BW and metabolic indicators of intermediate metabolism were recorded weekly. Behavior was recorded, and the analysis was conducted on wk 7 (preweaning) and 10 (postweaning). Solid feed intake increased at wk 7 and 8 when MH, LH, and CS were included in TMR; the same results were observed postweaning. The diets did not affect the average daily gain and BW, but the feed efficiency increased with the CON diet. The ß-hydroxybutyrate concentration was greater in calves receiving TMR-containing forage than CON diet. Furthermore, calves supplemented with forage had a greater rumination time. In conclusion, all forage sources included in the TMR showed feed intake and behavior benefits, reinforcing the need for fiber from forage in pre- and postweaning diets.

Different Conditions during Confinement in Pasture-Based Systems and Feeding Systems Affect the Fatty Acid Profile in the Milk and Cheese of Holstein Dairy Cows.

The diet of dairy cows influences the fatty acid (FA) profiles of their milk and cheese, but how these are affected by different conditions during confinement in a mixed system (MS:grazing + total mixed ration:TMR) is not known. The aim of this study was to compare the FAs of the milk and cheese from MS in a compost-bedded pack barns (CB-GRZ) versus an outdoor soil-bedded pen (OD-GRZ) during confinement, and with a confinement system (100%TMR) in a compost-bedded pack barns (CB-TMR). Individual milk samples (n = 12 cows/group), cheese, and pooled milk (MilkP) samples were collected. The saturated FA percentages in the milk and the omega 6/omega 3 ratio in the MilkP and cheese were greater for the CB-TMR (p < 0.0001), while the unsaturated and monounsaturated FA percentages in the milk were lower for the CB-TMR than the MS (p < 0.001). The milk n-3, C18:3, and conjugated linoleic acid percentages were lower for the CB-TMR than the MS (p < 0.001). The milk n-3 and C18:3 were higher for the CB-GRZ than the OD-GRZ (p < 0.01), but no differences were observed between the MS in the MilkP and cheese. In conclusion, CB-GRZ cows during confinement produced better quality milk compared to OD-GRZ cows. However, the FA profiles of the milk, MilkP, and cheese were affected to a greater extent by the feeding management than by the conditions during confinement.

Sugarcane total mixed ration silage ensiling with chitosan and homolactic microbial inoculant: characteristics of silage and animal digestion / Silagem de dieta total de cana de açúcar ensilada com quitosana e inoculante microbiano homoláctico: características da silagem e digestão animal

This study aimed to evaluate total mixed ration silages with sugarcane and the additives microbial inoculant and chitosan. Thirty mini-silos were used in a completely randomized design, with three treatments and ten replications. Silages were composed of sugarcane mixed with corn bran, whole soybean, urea, and mineral mixtures at a 50:50 roughage to concentrate ratio. Treatments consisted of control silage, microbial additive (Lactobacillus plantarum + Pediococcus acidilactici, 4 g/t of KeraSil, Kera Nutrição Animal), and chitosan (10 g/kg of natural matter). Silages were evaluated for fermentation and microbiological profile, fermentation losses, aerobic stability, chemical-bromatological composition, intake, and digestibility. Fermentation profile showed no significant difference between treatments for pH values, with a mean value of 4.79. Production of acetic and propionic acids showed no difference between treatments, with mean values of 7.34 and 0.053 mmol/kg DM, respectively. Dry matter, organic matter, and crude protein intake of the total mixed ration silage differed statistically from the other treatments (P<0.05), but fresh sugarcane and sugarcane silage intake did not differ from each other (P>0.05). Digestibility values of DM, OM, and NDF were higher in the total mixed ration silage (P<0.05), while sugarcane silage and fresh sugarcane showed no difference from each other (P>0.05). Total mixed ration silage increased nutrient intake and digestibility, with a better fermentation pattern when added with the microbial inoculant.

O objetivo foi avaliar as silagens de ração total com cana de açúcar e aditivos inoculante microbiano e quitosana. Foram utilizados trinta mini-silos utilizando o delineamento inteiramente casualizado, com três tratamentos e dez repetições. As silagens foram compostas por cana-de-açúcar misturada com farelo de milho, soja integral, uréia e misturas minerais na proporção de 50:50. Os tratamentos foram: silagem controle, aditivo microbiano (Lactobacillus plantarum + Pediococcus acidilactici, 4 g/t de KeraSil, Kera Nutrição Animal) e quitosana (10 g/kg da matéria natural). As silagens foram avaliadas quanto à fermentação e perfil microbiológico, perdas de fermentação, estabilidade aeróbica, composição químico-bromatológica, consumo e digestibilidade. O perfil de fermentação não mostrou diferença significativa entre tratamentos para os valores de pH, com um valor médio de 4,79. A produção de ácidos acéticos e propiônicos não mostrou diferença entre os tratamentos, com valores médios de 7,34 e 0,053 mmol/kg DM, respectivamente. A matéria seca, a matéria orgânica e a ingestão de proteína bruta da mistura total de ração silagem diferiram estatisticamente dos outros tratamentos (P<0,05), mas a ingestão de cana-de-açúcar fresca e de silagem de cana-de-açúcar não diferiram uma da outra (P>0,05). Os valores de digestibilidade de DM, OM e NDF foram maiores na silagem de ração mista total (P<0,05), enquanto a silagem de cana de açúcar e a cana de açúcar fresca não mostraram diferença entre si (P>0,05). A silagem de ração mista total aumentou a ingestão de nutrientes e a digestibilidade, com um melhor padrão de fermentação quando adicionado o inoculante microbiano.

Use of by-products in a total mixed ration silage / Uso de subprodutos na silagem de ração total misturada

The objective of this study was to evaluated fermentation losses and silage quality with the addition of different by-products in TMR. A completely randomized design was used with four treatments: Control (CTL, with hay, corn silage, cracked corn, soybean meal, wheat bran and minerals); CTL plus the addition of fresh orange pulp (FOP); CTL plus soybean hulls (SBH) or CTL plus wet brewery wastes (WBW), with six replicates. The ingredients were mixed and ensiled in 24 PVC experimental silos. After 56 days of ensiling, fermentation losses (effluents, dry matter and gases), and the TMR silage pH and chemical composition were analyzed; aerobic stability was evaluated over seven days after the silos were opened. Lower dry matter (P ≤ 0.0001) was observed in TMR ensiled with wet (WBW and FOP) compared to dry (SBH) by-products. Greater ADF (P = 0.031) was observed in SBH when compared to others by-products. WBW and FOP increased dry matter (P ≤ 0.0001) and effluent losses (P ≤ 0.0001) when compared to CTL and SBH. SBH treatment remained stable after 176-hour, which differ (P=0.0015) compared with the other treatments (range from 94.5 to 99 hours of exposure to air). The density (kg DM/m3) changed (P ≤ 0.0001) among treatments. The inclusion of SBH in TMR silages had the greatest density, followed by CTL, WBW and FOP. The density was negatively correlated with DM losses ( r= - 0,81; P ≤ 0.0001). The pH differ (P = 0.003) among treatments. Highest pH of TMR silage was observed for SBH (3,67) and the lowest (3,56) for FOP. Soybean hulls successful improved aerobic stability and density, whereas aerobic stability and density were decreased and fermentation losses increased when fresh orange pulp and wet brewery were used. Little effects on chemical composition were observed, thereby agro-industrial by-products can be used in TMR silages as long as there is adequate product availability and pricing.(AU)

Objetivou-se avaliar as perdas fermentativas e qualidade química da ração total misturada (TMR) ensilada com a adição de diferentes subprodutos da indústria. Um delineamento inteiramente casualizado com quatro tratamentos foi utilizado: CTL (Feno, silagem de milho, milho quirera, farelo de soja, farelo de trigo e mistura mineral), FOP (CTL com adição de bagaço de laranja in-natura), ou SBH (CTL com adição de casca de soja) e WBW (CTL com adição de resíduo úmido de cervejaria), utilizando-se seis repetições por tratamento. Os ingredientes foram misturados e armazenados em 24 silos experimentais de PVC. Após 56 dias de armazenamento, as perdas fermentativas (efluentes, perda de matéria seca e produção de gases), e o pH, composição bromatológica e estabilidade aeróbica durante 7 dias das silagens de TMR foram mensuradas. Menor teor de MS (P ≤ 0,0001) foi observado para TMR ensiladas com resíduos úmidos (WBW e FOP) comparado com SBH. O uso de SBH apresentou maior teor de FDA (P = 0,031) comparado aos demais tratamentos. Adição de WBW e FOP aumentou as perdas de matéria seca (P ≤ 0,0001) e efluentes (P ≤ 0,0001) em relação a CTL e SBH. O tratamento SBH não apresentou quebra de estabilidade (176 horas), diferindo (P = 0,0015) em relação aos outros tratamentos (variação de 94,5 a 99 horas de exposição ao ar). A densidade (kg MS/m³) foi diferente (P ≤ 0.0001) entre os tratamentos. A inclusão de SBH na silagem de TMR apresentou as maiores densidades, seguido pelo tratamento CTL, WBW e FOP. A densidade teve correlação negative com as perdas de MS ( r= -0,81; P ≤ 0.0001). O pH diferiu entre tratamento (P = 0,003), sendo maior para CSC (3,67) e menor para BLI (3,56). O tratamento casca de soja apresentou a melhor estabilidade aeróbia e densidade, enquanto a estabilidade e densidade diminuíram e as perdas fermentativas aumentaram quando bagaço de laranja in-natura e resíduo úmido de cervejaria foram utilizados. Apesar das diferenças na composição das [...].(AU)

Use of by-products in a total mixed ration silage / Uso de subprodutos na silagem de ração total misturada

The objective of this study was to evaluated fermentation losses and silage quality with the addition of different by-products in TMR. A completely randomized design was used with four treatments: Control (CTL, with hay, corn silage, cracked corn, soybean meal, wheat bran and minerals); CTL plus the addition of fresh orange pulp (FOP); CTL plus soybean hulls (SBH) or CTL plus wet brewery wastes (WBW), with six replicates. The ingredients were mixed and ensiled in 24 PVC experimental silos. After 56 days of ensiling, fermentation losses (effluents, dry matter and gases), and the TMR silage pH and chemical composition were analyzed; aerobic stability was evaluated over seven days after the silos were opened. Lower dry matter (P ≤ 0.0001) was observed in TMR ensiled with wet (WBW and FOP) compared to dry (SBH) by-products. Greater ADF (P = 0.031) was observed in SBH when compared to others by-products. WBW and FOP increased dry matter (P ≤ 0.0001) and effluent losses (P ≤ 0.0001) when compared to CTL and SBH. SBH treatment remained stable after 176-hour, which differ (P=0.0015) compared with the other treatments (range from 94.5 to 99 hours of exposure to air). The density (kg DM/m3) changed (P ≤ 0.0001) among treatments. The inclusion of SBH in TMR silages had the greatest density, followed by CTL, WBW and FOP. The density was negatively correlated with DM losses ( r= - 0,81; P ≤ 0.0001). The pH differ (P = 0.003) among treatments. Highest pH of TMR silage was observed for SBH (3,67) and the lowest (3,56) for FOP. Soybean hulls successful improved aerobic stability and density, whereas aerobic stability and density were decreased and fermentation losses increased when fresh orange pulp and wet brewery were used. Little effects on chemical composition were observed, thereby agro-industrial by-products can be used in TMR silages as long as there is adequate product availability and pricing.

Objetivou-se avaliar as perdas fermentativas e qualidade química da ração total misturada (TMR) ensilada com a adição de diferentes subprodutos da indústria. Um delineamento inteiramente casualizado com quatro tratamentos foi utilizado: CTL (Feno, silagem de milho, milho quirera, farelo de soja, farelo de trigo e mistura mineral), FOP (CTL com adição de bagaço de laranja in-natura), ou SBH (CTL com adição de casca de soja) e WBW (CTL com adição de resíduo úmido de cervejaria), utilizando-se seis repetições por tratamento. Os ingredientes foram misturados e armazenados em 24 silos experimentais de PVC. Após 56 dias de armazenamento, as perdas fermentativas (efluentes, perda de matéria seca e produção de gases), e o pH, composição bromatológica e estabilidade aeróbica durante 7 dias das silagens de TMR foram mensuradas. Menor teor de MS (P ≤ 0,0001) foi observado para TMR ensiladas com resíduos úmidos (WBW e FOP) comparado com SBH. O uso de SBH apresentou maior teor de FDA (P = 0,031) comparado aos demais tratamentos. Adição de WBW e FOP aumentou as perdas de matéria seca (P ≤ 0,0001) e efluentes (P ≤ 0,0001) em relação a CTL e SBH. O tratamento SBH não apresentou quebra de estabilidade (176 horas), diferindo (P = 0,0015) em relação aos outros tratamentos (variação de 94,5 a 99 horas de exposição ao ar). A densidade (kg MS/m³) foi diferente (P ≤ 0.0001) entre os tratamentos. A inclusão de SBH na silagem de TMR apresentou as maiores densidades, seguido pelo tratamento CTL, WBW e FOP. A densidade teve correlação negative com as perdas de MS ( r= -0,81; P ≤ 0.0001). O pH diferiu entre tratamento (P = 0,003), sendo maior para CSC (3,67) e menor para BLI (3,56). O tratamento casca de soja apresentou a melhor estabilidade aeróbia e densidade, enquanto a estabilidade e densidade diminuíram e as perdas fermentativas aumentaram quando bagaço de laranja in-natura e resíduo úmido de cervejaria foram utilizados. Apesar das diferenças na composição das [...].

Productive performance and digestive response of dairy cows fed different diets combining a total mixed ration and fresh forage.

The purpose of this experiment was to determine the effects of feeding increasing levels of fresh forage (FF) as a proportion of total dry matter intake (DMI) on nutrient intake, rumen digestion, nutrient utilization, and productive performance of total mixed ration (TMR)-fed cows. Twelve dairy cows (90 ± 22 d in milk, 523 ± 88 kg of body weight, 7,908 ± 719 kg of milk production in the previous lactation) were housed in individual tiestalls and assigned to treatments according to a 3 × 3 Latin square design replicated 4 times. Treatments were 100% TMR (T100), 75% TMR plus 25% FF (T75), and 50% TMR plus 50% FF (T50). The experiment lasted 60 d, divided into 3 periods of 20 d each; the first 12 d of each period were used for diet adaptation and the last 8 d for data collection. The TMR (18.1% crude protein, 24.6% acid detergent fiber) and FF (Lolium multiflorum; 15.1% crude protein, 24.1% acid detergent fiber) were prepared and cut daily and offered to each cow individually. The highest DMI was reached in T100 and T75, which was reflected in greater intake of the different nutrients than T50. No differences were detected in the apparent total digestibility of the nutrients, mean ruminal pH, and total volatile fatty acid concentrations among treatments. Cows in T50 resulted in the lowest ruminal N-NH3 concentration and the lowest microbial N flow to the duodenum. Milk yield was 8.5% higher from cows in T100 and T75 compared with T50, but we observed no differences for milk fat or milk protein yield among treatments. Milk fat of cows fed T50 had 8% more unsaturated fatty acids (FA) than that of cows fed T100, mostly because of a higher content of monounsaturated FA. Additionally, cows in T50 had a higher concentration of linoleic acid, vaccenic acid, and rumenic acid than T100. Meanwhile, the concentration of linoleic acid and vaccenic acid in cows fed T75 was higher than T100. The milk fat of the cows fed T50 and T75 had a lower n-6:n-3 ratio than T100. We concluded that including up to 29% of FF in the total DMI in combination with a TMR did not affect the intake or digestion of nutrients or the productive response in dairy cows and resulted in a higher concentration of desirable FA from a consumer's perspective.

Digestive response of dairy cows fed diets combining fresh forage with a total mixed ration.

The objective of this experiment was to quantify the response of dairy cows fed a total mixed ration (TMR) to increasing access to high-quality temperate fresh forage with respect to energy intake, rumen fermentation, microbial protein flow, passage rate, nutrient digestion and utilization, and metabolic and endocrine profiles. Nine Holstein cows fed a TMR were assigned to the following treatments according to a 3×3 Latin square replicated 3 times with 20-d periods and sampling on the last 10 d of each period: 0 (T0), 4 (T4), or 8 (T8) h of daily access to fresh forage. The forage (Lolium multiflorum; 17.1% crude protein, 26.5% acid detergent fiber) was cut daily and offered ad libitum beginning at 0800h, and a TMR (16.1% crude protein, 22.9% acid detergent fiber) was offered ad libitum during the remaining time. Energy intake and balance were higher in T0 than in T8, which was reflected in higher blood glucose and insulin concentrations in T0. Total volatile fatty acid concentrations in the rumen were higher in T0 and T4 than in T8, pH was lower in T4 than in T8, and ammonia-N was higher in T0 than in T8. No differences among treatments were detected in microbial protein flow to the duodenum, digestibility of nutrients, apparent efficiency of energy, or N utilization for milk production, but the total mean retention time of feed in the digestive tract was higher in T8 than in T0. It is concluded that more than 4h of daily access to high-quality fresh forage in the diet of dairy cows fed a TMR reduced energy intake and balance but had no effects on nutrient digestion or utilization.

Short communication: Intake, milk production, and milk fatty acid profile of dairy cows fed diets combining fresh forage with a total mixed ration.

To establish the effects of access time to high-quality temperate fresh forage (FF) on intake and performance of cows fed a total mixed ration (TMR), 9 Holstein cows were assigned to three 3 × 3 Latin squares with 20-d periods with sampling in the last 10 d. The following treatments were evaluated: 0 (T0), 4 (T4), and 8 (T8) h of daily access to FF. Forage (Lolium multiflorum) was cut daily and offered ad libitum beginning at 0800 h in individual stalls, and a TMR was offered ad libitum during the period when cows had no access to FF. Fresh forage dry matter intake (DMI) increased and TMR DMI decreased with the access time to FF, whereas total DMI was 3.0 kg higher for T4 than for T8; no differences were detected with T0. Compared with T0, 8h/d of access to FF decreased milk, protein, and casein yields, and tended to decrease fat and lactose yields, but treatment had no effect on milk composition or feed efficiency. The milk fatty acid profile in T8 had a higher content of rumenic, vaccenic, and linolenic acids compared with T0. We concluded that cows with 4h of access to high-quality FF had similar DMI and milk yields as cows fed only the TMR, but more than 4h of access reduced DMI and performance without changes in feed efficiency, although milk fat had enhanced levels of beneficial fatty acids.

Behavior of sheep fed different sequences of ingredients in a spineless cactus (Nopalea cochenillifera Salm-Dyck) based-diet

The purpose of this experiment was to evaluate the ingestive behavior of sheep fed diets containing different sequences of ingredients of a spineless cactus based diet because of the paucity of available information regarding the best method of feeding, especially in relation to the use of spineless cactus. The observed variables were daily time of eating, ruminating and idling. Five sheep were distributed into a 5 × 5 Latin Square with five treatments, five animals and five periods, the treatments 1 - (TMR, total mixed ration) at 7:00 and 15:00h; 2 - (C, concentrate) at 7:00h, (SC, spineless cactus) at 8:00 h, (C) at 15:00h and (TGH, tifton grass hay) at 16:00h; 3 - (C) at 7:00h, (TGH) at 8:00h, (C) at 15:00h and (SC) at 16:00h; 4 - (SC) at 7:00h, (C) at 8:00h, (TGH) at 15:00 and (C) at 16:00h and 5 - (TGH) at 7:00h, (C) at 8:00h, (SC) at 15:00h and (C) at 16:00h. No treatment effect on feeding activities (hour/day) in time total daily, but when activities were divided into periods, the sheep in sequence 3: concentrate/ hay/ concentrate/spineless cactus spent a longer time eating within the period from 7:00 to 11:00h than the sheep in sequences 1: total mixed ration (TMR) and 2: concentrate/ spineless cactus/ concentrate /hay. The digestibility of dry matter and matter organic was greater for concentrate/ spineless cactus/ concentrate/ hay and spineless cactus/ concentrate/ hay/ concentrate being recommended the use of the same as feeding strategy.

Objetivou-se com este estudo avaliar o comportamento ingestivo de ovinos alimentados com dietas contendo diferentes sequências de ingredientes a base de palma forrageira, devido à escassez de informações disponíveis sobre o melhor método de alimentação, especialmente ao uso da palma forrageira. As variáveis observadas foram tempo diário de ingestão, ruminação e ócio. Cinco ovinos foram distribuídos em um quadrado latino 5 × 5, com cinco tratamentos, cinco animais e cinco períodos, sendo os tratamentos: 1 - (ração totalmente misturada- TMR) às 7:00 e às 15:00h; 2 - (concentrado- C) às 7:00h, (palma forrageira- PF) às 8:00h, (C) às 15:00h e (feno de capim tifton- FCT) às 16:00h; 3 - (C) às 7:00h, (FCT) às 8:00h, (C) às 15:00h e (PF) às 16:00h; 4 - (PF) às 7:00h, (C) às 8:00h, (FCT) às 15:00 e (C) às 16:00h e 5 - (FCT) às 7:00h, (C) às 8:00h, (PF) às 15:00h e (C) às 16:00h. Não houve efeito de tratamento sobre as atividades de alimentação (horas/dia) no total de tempo diário. Porém quando as atividades foram divididas em períodos, os ovinos na sequência 3: concentrado/ feno/ concentrado/ palma passaram um tempo de ingestão maior no período de 7:00-11:00 h comparados aos da sequência 1: ração total misturada e 2: concentrado/ palma/ concentrado/ feno. A digestibilidade da matéria seca e da matéria orgânica foi maior para concentrado/ palma/ concentrado/ feno e palma/concentrado/feno/concentrado sendo recomendado o uso dos mesmos como estratégia alimentar.