RESUMEN
BACKGROUND: Coffee is one of the most frequently consumed beverages worldwide. Research on effects of coffee drinking has focused on caffeine; however, coffee contains myriad biochemicals that are chemically unrelated to caffeine, including 3,4-dihydroxyphenyl compounds (catechols) such as caffeic acid and dihydrocaffeic acid (DHCA). OBJECTIVE: This prospective within-subjects study examined effects of drinking caffeinated or decaffeinated coffee on plasma free (unconjugated) catechols measured by liquid chromatography with series electrochemical detection (LCED) after batch alumina extraction. To confirm coffee-related chromatographic peaks represented catechols, plasma was incubated with catechol-O-methyltransferase and S-adenosylmethionine before the alumina extraction; reductions in peak heights would identify catechols. METHODS: Ten healthy volunteers drank 2 cups each of caffeinated and decaffeinated coffee on separate days after fasting overnight. With subjects supine, blood was drawn through an intravenous catheter up to 240 min after coffee ingestion and the plasma assayed by alumina extraction followed by LCED. RESULTS: Within 15 min of drinking coffee of either type, >20 additional peaks were noted in chromatographs from the alumina eluates. Most of the coffee-related peaks corresponded to free catechols. Plasma levels of the catecholamines epinephrine and dopamine increased with both caffeinated and decaffeinated coffee. Levels of other endogenous catechols were unaffected. Plasma DHCA increased bi-phasically, in contrast with other coffee-related free catechols. INTERPRETATION: Drinking coffee-whether caffeinated or decaffeinated-results in the rapid appearance of numerous free catechols in the plasma. These might affect the disposition of circulating catecholamines. The bi-phasic increase in plasma DHCA is consistent with production by gut bacteria.
Asunto(s)
Cafeína/análisis , Catecoles/sangre , Café/metabolismo , Adulto , Ácidos Cafeicos/sangre , Cafeína/metabolismo , Café/química , Femenino , Humanos , Masculino , Plasma/química , Estudios Prospectivos , Adulto JovenRESUMEN
Rosmarinic acid (RA), an ester compound of caffeic acid (CA) and 3,4-dihydroxyphenyllacic acid, is widely distributed in the herbs of the Lamiaceae family and has shown a wide spectrum of pharmacological properties. CA and FA (ferulic acid) are two bioactive metabolites in vivo after oral administration of RA; however, a rapid and robust analytical approach that can enable the quantitative assay of RA and two bioactive metabolites is still lacking. A liquid chromatography/tandem mass spectrometry method was established that was capable of the quantitative determination of RA, CA and FA by negative-mode multiple reaction monitoring within 7 min using a Zorbax SB-C18 column and an isocratic elution. This assay method was validated as linear over the investigated ranges with correlation coefficients (r) > 0.9950. The intra- and inter-day precision was <10.65%, and the accuracies (relative error, %) <-6.41%. The validated approach was applied to a pharmacokinetics study of RA and its two metabolites in rats after oral and intravenous administration. RA was rapidly metabolized in both administration modes, whilst the metabolites CA and FA were only detectable by oral administration. The absolute availability of RA was calculated to be 4.13%.
Asunto(s)
Ácidos Cafeicos/sangre , Cromatografía Liquida/métodos , Cinamatos/sangre , Ácidos Cumáricos/sangre , Depsidos/sangre , Espectrometría de Masas en Tándem/métodos , Animales , Ácidos Cafeicos/química , Ácidos Cafeicos/farmacocinética , Cinamatos/química , Cinamatos/farmacocinética , Ácidos Cumáricos/química , Ácidos Cumáricos/farmacocinética , Depsidos/química , Depsidos/farmacocinética , Modelos Lineales , Masculino , Ratas , Ratas Sprague-Dawley , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Ácido RosmarínicoRESUMEN
Qi-Shen-Ke-Li (QSKL), a traditional Chinese formula prepared from six herbs, has long been used for the treatment of coronary heart disease and chronic heart failure. However, the herbal combination mechanism and underlying material basis of this multi-herbal formula are not clear. In this study, an ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to simultaneously determine multiple bioactive compounds in QSKL was established and validated. Using the developed method, 18 bioactive components in rat plasma after oral administration of QSKL formula and its single herb extracts were quantified. Based on these results, pharmacokinetic (PK) parameters (T1/2 , Tmax , Cmax , AUC0-48h , and AUC0-∞ ) of the 18 bioactive components were analyzed and compared using PKSlover 2.0 PK software. The experimental data suggested that significant changes in PK profiles were observed between the QSKL formula and its single-herb extracts. The herbal combination in QSKL significantly influences the system exposure and the PK behaviors of the 18 bioactive components, indicating multicomponent interactions among the herbs. This study provides insight into the herbal combination mechanism and underlying material basis of the QSKL formula.
Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Medicamentos Herbarios Chinos , Espectrometría de Masas en Tándem/métodos , Administración Oral , Animales , Disponibilidad Biológica , Ácidos Cafeicos/sangre , Ácidos Cafeicos/química , Ácidos Cafeicos/farmacocinética , Diterpenos/sangre , Diterpenos/química , Diterpenos/farmacocinética , Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacocinética , Flavonoides/sangre , Flavonoides/química , Flavonoides/farmacocinética , Lactatos/sangre , Lactatos/química , Lactatos/farmacocinética , Límite de Detección , Modelos Lineales , Masculino , Ratas , Ratas Sprague-Dawley , Reproducibilidad de los ResultadosRESUMEN
OBJECTIVE: To determine the constituent compounds of Danggui buxue decoction (DBD) involved and the potential mechanisms mediating its effects, with specific reference to lipids playing a role in the initiation of diabetic atherosclerosis. METHODS: Liquid chromatography-tandem mass spectrometry was used to identify and quantify the absorbed bioactive compounds (ABCs) present in DBD. Goto-Kakizaki (GK) rats were randomly allocated to a diabetes atherosclerosis (DA) group, a DBD group, and an ABC group (10 per group), which were all high-fat diet-fed. The treated rats were administered DBD (4 g/kg) or ABCs (in amounts equal to those present in DBD) once daily for 28 d, and a control group of Wistar rats were administered vehicle. Body mass gain, fasting blood glucose, and homeostasis assessment of insulin resistance (HOMA- IR) were measured. Serum triglyceride (TG), cholesterol (CHOL), high density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL- C) and tumor necrosis factor-α (TNF-α) concentrations were determined. Hematoxylin and eosin staining and microscopy were used to characterize the abdominal aorta and the expression of lipogenic genes was quantified in this vessel. RESULTS: Seven ABCs were identified in rat serum: ferulic acid, formononetin, calycosin, astragaloside, caffeic acid, ligustilide, and butyphthalide. DBD significantly reduced HOMA-IR, the serum concentrations of TG, CHOL, and LDL-C, and the expression of the lipogenic genes monocyte chemotactic protein 1, Fas, intercellular adhesion molecule 1, and Cd36 in aorta; and significantly increased the mRNA expression of Scd1 in aorta. CONCLUSION: DBD affects lipid metabolism in the early stage of atherosclerosis in diabetic GK rats, with the mechanism likely involving the regulation of lipid metabolic genes in vessels. The contribution of ABCs to the effect of DBD on lipid metabolism was 24%-101%.
Asunto(s)
Aterosclerosis/tratamiento farmacológico , Aterosclerosis/metabolismo , Diabetes Mellitus Tipo 2/complicaciones , Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/química , Metabolismo de los Lípidos/efectos de los fármacos , Animales , Aterosclerosis/etiología , Aterosclerosis/genética , Ácidos Cafeicos/administración & dosificación , Ácidos Cafeicos/sangre , Ácidos Cumáricos/administración & dosificación , Ácidos Cumáricos/sangre , Humanos , Masculino , Ratas , Ratas WistarRESUMEN
Epidemiological data indicate that a diet rich in plant polyphenols has a positive effect on brain functions, improving memory and cognition in humans. Direct activity of ingested phenolics on brain neurons may be one of plausible mechanisms explaining these data. This also suggests that some phenolics can cross the blood-brain barrier and be present in the brain or cerebrospinal fluid. We measured 12 phenolics (a combination of the solid-phase extraction technique with high-performance liquid chromatography) in cerebrospinal fluid and matched plasma samples from 28 patients undergoing diagnostic lumbar puncture due to neurological disorders. Homovanillic acid, 3-hydroxyphenyl acetic acid and caffeic acid were detectable in cerebrospinal fluid reaching concentrations (median; interquartile range) 0.18; 0.14 µmol/L, 4.35; 7.36 µmol/L and 0.02; 0.01 µmol/L, respectively. Plasma concentrations of caffeic acid (0.03; 0.01 µmol/L) did not correlate with those in cerebrospinal fluid (ρ = -0.109, p = 0.58). Because food (fruits and vegetables) is the only source of caffeic acid in human body fluids, our results indicate that the same dietary phenolics can cross blood-brain barrier in humans, and that transportation of caffeic acid through this barrier is not the result of simple or facilitated diffusion.
Asunto(s)
Barrera Hematoencefálica/efectos de los fármacos , Ácidos Cafeicos/sangre , Ácidos Cafeicos/líquido cefalorraquídeo , Ácidos Cafeicos/farmacología , Polifenoles/farmacología , Adulto , Barrera Hematoencefálica/metabolismo , Cromatografía Líquida de Alta Presión , Dieta Occidental , Femenino , Frutas/química , Ácido Homovanílico/sangre , Ácido Homovanílico/líquido cefalorraquídeo , Humanos , Masculino , Persona de Mediana Edad , Enfermedades del Sistema Nervioso/sangre , Enfermedades del Sistema Nervioso/líquido cefalorraquídeo , Polifenoles/sangre , Polifenoles/líquido cefalorraquídeo , Extracción en Fase Sólida , Verduras/químicaRESUMEN
Aim: FLZ, a novel promising dopamine neuroprotective agent, is designed to treat Parkinson's disease. F7G and F21G are FLZ major active Phase II metabolites whose exposure are nearly 100-times higher than FLZ, may chiefly produce effectiveness in human. Measurement of F7G and F21G in plasma samples is critical for investigating its pharmacokinetics in clinical studies. Methodology & results: Plasma samples were extracted by SPE method and then analyzed by a newly established ultra-UHPLC-MS/MS method. Conclusion: For the first time, a reliable and robust bioanalytical method for F7G and F21G detection was successfully applied in a first-in-human study.
Asunto(s)
Fármacos Neuroprotectores , Acrilamidas/sangre , Acrilamidas/metabolismo , Acrilamidas/farmacocinética , Ácidos Cafeicos/sangre , Ácidos Cafeicos/metabolismo , Ácidos Cafeicos/farmacocinética , Cromatografía Líquida de Alta Presión , Humanos , Conformación Molecular , Fármacos Neuroprotectores/sangre , Fármacos Neuroprotectores/metabolismo , Fármacos Neuroprotectores/farmacocinética , Estereoisomerismo , Espectrometría de Masas en TándemRESUMEN
BACKGROUND AND OBJECTIVES: As a traditional Chinese Materia Medica (CMM), the Compound Danshen Dripping Pill (CDDP) is widely used for the treatments of cardiovascular diseases. In view of its undefined applicable population and dosage, a population pharmacokinetic (PPK) study is required. The objective of this study was to explore the feasibility of multi-component CMM PPK in rat plasma after oral administration of CDDP based on sparse sampling. METHODS: In this research, a simple, rapid and highly sensitive UFLC-MS/MS method for the simultaneous determination of tanshinol (TSL), ginsenoside Rb1 (GRb1) and ginsenoside Rg1 (GRg1) has been successfully developed in rat plasma. Moreover, the validated method has been applied to a PPK study of CDDP based on sparse data. We established the PPK models for these three main active constituents using a nonlinear mixed-effects model, taking into account of factors such as gender, age in weeks and weight. RESULTS: The PPK models of TSL and GRb1 were best described by a one-compartment model with linear elimination and first-order absorption. The model of GRg1 was best described by a two-compartment model with first-order absorption. Bootstrap validation and a visual predictive check confirmed the predictive ability, the model stability and the precision of the parameter estimates from these models. CONCLUSION: As a preliminary exploration toward the clinical population pharmacokinetic research, this study provides a reference for the population pharmacokinetic study of traditional CMM.
Asunto(s)
Ácidos Cafeicos/farmacocinética , Cromatografía Liquida , Medicamentos Herbarios Chinos/farmacocinética , Ginsenósidos/farmacocinética , Modelos Biológicos , Espectrometría de Masas en Tándem , Administración Oral , Animales , Ácidos Cafeicos/administración & dosificación , Ácidos Cafeicos/sangre , Canfanos , Medicamentos Herbarios Chinos/administración & dosificación , Estudios de Factibilidad , Femenino , Ginsenósidos/administración & dosificación , Ginsenósidos/sangre , Humanos , Masculino , Panax notoginseng , Ratas Wistar , Salvia miltiorrhizaRESUMEN
To investigate the effect of ligustrazine on the pharmacokinetic profile of tanshinol after intravenous administration in rats, a sensitive liquid chromatography tandem mass spectrometry method was developed and validated for quantitative determination of tanshinol and ligustrazine in rat plasma. After prepared by protein precipitation, the analytes were separated on a Waters Acquity HSS T3 column (100 × 2.1 mm, 1.8µm) and eluted by 0.1% formic acid in water and acetonitrile at a flow rate of 0.4 ml/min. The precursor-product ion transitions were m/z 197.0 â 135.0 for tanshinol, m/z 417.1 â 255.1 for liquiritin (internal standard) in negative ion mode and m/z 137.1 â 55.0 for ligustrazine in positive ion mode. To avoid the interference of tanshinol metabolite transformation, the stability of analytes in samples collected after administration was assessed. The validated method was successfully applied to a pharmacokinetic study after intravenous administration of single tanshinol and Danshen Chuanxiongqin Injection. After Danshen Chuanxiongqin injection administration, the values of elimination half-time, area under the concentration-time curve and Co were 0.36 ± 0.13 h, 1.29 ± 0.37 µg/ml h and 10.51 ± 2.58 µg/ml for male rats, respectively. In the single tanshinol group, the corresponding values were 0.56 ± 0.24 h, 1.85 ± 0.44 µg/ml h and 14.11 ± 2.26 µg/ml for male rats-30-40% higher than those for the Danshen Chuanxiongqin Injection group. There was a significant different between male and female rats. This study provided information on the influence of ligustrazine on the pharmacokinetic characteristics of tanshinol after intravenous administration of Danshen Chuanxiongqin Injection in rats, which will be helpful for its clinical application.
Asunto(s)
Ácidos Cafeicos , Pirazinas , Administración Intravenosa , Animales , Ácidos Cafeicos/administración & dosificación , Ácidos Cafeicos/sangre , Ácidos Cafeicos/química , Ácidos Cafeicos/farmacocinética , Cromatografía Líquida de Alta Presión/métodos , Femenino , Modelos Lineales , Masculino , Pirazinas/administración & dosificación , Pirazinas/sangre , Pirazinas/química , Pirazinas/farmacocinética , Ratas , Ratas Sprague-Dawley , Reproducibilidad de los Resultados , Salvia miltiorrhiza , Sensibilidad y Especificidad , Espectrometría de Masas en Tándem/métodosRESUMEN
Tuberculosis of cervical lymph nodes is called scrofula in Traditional Chinese Medicine (TCM). Clinical manifestation is that unilateral or bilateral neck can have multiple enlarged lymph nodes of different sizes. Current therapeutic drugs include Lysionotus pauciflorus Maxim. tablets and compound of Lysionotus pauciflorus Maxim., which have a significant effect on tuberculosis of cervical lymph nodes. This compound is composed of three herbs, Lysionotus pauciflorus Maxim., Prunella vulgaris L. and Artemisia argyi Levl.et Vant. A selective and sensitive LC-MS/MS method was established and validated in rat plasma for the first time. Chromatographic separation was achieved on a Wonda Cract ODS-2 C18 Column (150â¯mmâ¯×â¯4.6â¯mm, 5⯵m). The mobile phase contained 0.1% formic acid aqueous solution and acetonitrile with a flow rate of 0.8â¯mL/min. The detection was performed in negative electrospray ionization mode and the precursor/product ion transitions of six components and internal standard (IS) sulfamethoxazole were quantified in multiple reaction monitoring (MRM) using QTRAP-3200 MS/MS. The method fulfilled US Food and Drug Administration guidelines for selectivity, sensitivity, accuracy, precision, matrix effect, extraction recovery, dilution integrity, and stability. This proposed method was then successfully applied to a pharmacokinetic study after oral administration of 10â¯mL/kg compound extracts in rats. The pharmacokinetic parameters and plasma concentration-time profiles would prove valuable in pre-clinical and clinical investigations on the disposition of compound medicine.
Asunto(s)
Medicamentos Herbarios Chinos/análisis , Lamiales/química , Espectrometría de Masas en Tándem/métodos , Administración Oral , Animales , Ácidos Cafeicos/administración & dosificación , Ácidos Cafeicos/sangre , Ácidos Cafeicos/farmacocinética , Cromatografía Líquida de Alta Presión/métodos , Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/farmacocinética , Flavonas/administración & dosificación , Flavonas/sangre , Flavonas/farmacocinética , Glucósidos/administración & dosificación , Glucósidos/sangre , Glucósidos/farmacocinética , Masculino , Modelos Animales , Fenilpropionatos/administración & dosificación , Fenilpropionatos/sangre , Fenilpropionatos/farmacocinética , Ratas , Ratas Sprague-Dawley , Reproducibilidad de los Resultados , Espectrometría de Masa por Ionización de Electrospray/métodos , Comprimidos , Tuberculosis Ganglionar/tratamiento farmacológico , Ácido RosmarínicoRESUMEN
Gastric cancer is one of the most common cancers leading to tumor-related deaths worldwide. Chicoric acid (CA) exhibits a variety of protective effects in different diseases. However, its role in regulating tumor progression has not been reported. Autophagy, as a conserved catabolic process, sustains cellular homoeostasis responding to stress to modulate cell fate. In the study, the effects of CA on gastric cancer were investigated. The results indicated that CA treatment markedly reduced the cell viability and induced apoptosis in gastric cancer cells, and prevented tumor growth in an established xenograft gastric cancer model. Furthermore, CA exposure significantly induced autophagy both in gastric cancer cells and tumor samples, as evidenced by the up-regulated expression of LC3II. Moreover, phosphorylated AMP-activated protein kinase (AMPK) and p70S6 kinase (p70s6k) expression were obviously promoted by CA in vitro and in vivo. Importantly, blocking AMPK activation abrogated CA-induced expression of LC3II in gastric cancer cells. In addition, endoplasmic reticulum (ER) stress in tumor samples or cells was markedly induced by CA treatment through promoting the expression of associated signals such as Parkin, protein kinase RNA-like ER kinase (PERK), activating transcription factors 4 (ATF4) and ATF6. Importantly, these effects were abolished by the inhibition of AMPK signaling. Collectively, our findings indicated that CA prevents human gastric cancer progression by inducing autophagy partly through the activation of AMPK, and represents an effective therapeutic strategy against gastric cancer development.
Asunto(s)
Proteínas Quinasas Activadas por AMP/metabolismo , Anticarcinógenos/farmacología , Autofagia/efectos de los fármacos , Ácidos Cafeicos/farmacología , Estrés del Retículo Endoplásmico/efectos de los fármacos , Células Epiteliales/efectos de los fármacos , Neoplasias Gástricas/prevención & control , Succinatos/farmacología , Animales , Anticarcinógenos/sangre , Ácidos Cafeicos/sangre , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Células Epiteliales/metabolismo , Células Epiteliales/patología , Humanos , Ratones Endogámicos BALB C , Ratones Desnudos , Ratas , Ratas Sprague-Dawley , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patología , Succinatos/sangreRESUMEN
Acanthus ilicifolius herb (AIH), the dry plant of Acanthus ilicifolius L., has long been used as a folk medicine for treating acute and chronic hepatitis. Phenylethanoid glycosides (PhGs) are one family of the main components in AIH with hepatoprotective, antioxidant, and anti-inflammatory activities. In this study, the pharmacokinetics of AIH was investigated preliminarily by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-MS/MS). A simultaneously quantitative determination method for four PhGs (acteoside, isoacteoside, martynoside, and crenatoside) in rat plasma was first established by UPLC-MS/MS. These four PhGs were separated with an ACQUITY UPLC BEH C18 column (2.1 × 50 mm, 1.7 µm) by gradient elution (mobile phase: MeCN and 0.1% formic acid in water, 0.4 mL/min). The mass spectrometry detection was performed using negative electrospray ionization (ESI-) in multiple reaction monitoring (MRM) mode. By the established method, the preliminary pharmacokinetics of AIH was elucidated using the kinetic parameters of the four PhGs in rat plasma after intragastric administration of AIH ethanol extract. All four PhGs showed double peaks on concentration-time curves, approximately at 0.5 h and 6 h, respectively. Their elimination half-lives (t1/2) were different, ranging from 3.42 h to 8.99 h, although they shared similar molecular structures. This work may provide a basis for the elucidation of the pharmacokinetic characteristics of bioactive components from AIH.
Asunto(s)
Acanthaceae/química , Glicósidos/sangre , Extractos Vegetales/administración & dosificación , Animales , Ácidos Cafeicos/sangre , Ácidos Cafeicos/farmacocinética , Cromatografía Liquida , Glucósidos/sangre , Glucósidos/farmacocinética , Glicósidos/farmacocinética , Masculino , Fenoles/sangre , Fenoles/farmacocinética , Extractos Vegetales/farmacocinética , Ratas , Ratas Sprague-Dawley , Espectrometría de Masas en TándemRESUMEN
Dengzhan Shengmai Capsule (DZSMC) is a traditional Chinese medicine (TCM) formula with remarkable clinical effect in the treatment of stroke sequelae. Exploring the components of DZSMC and detecting the absorbed prototype constituents and metabolites in blood are of great significance to clarify the effective substances of this prescription. Here, a reliable method using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) was established for the comprehensive analysis of chemical constituents of DZSMC and their metabolites in rat plasma after gastric perfusion. Two acquisition modes, including MSE mode and Fast DDA mode, were performed for acquiring more precursor ions and cleaner precursor-product ions background during the study of constituents of DZSMC. As a result, a total of 125 constituents were unambiguously characterized or tentatively identified. For the first time, a total of 92 components, including 44 prototype components and 48 metabolites were unambiguously or tentatively identified in rat plasma. The metabolic pathways included phase I reactions (hydration, hydrogenation, oxidation, demethylation and hydroxylation) and phase II reactions (conjugation with glucuronide, sulfate and methyl). Furthermore, the metabolites from caffeic acid and scutellarin were characterized and validated by phase II metabolic reactions in vitro, which could be established as a simulated in vivo environment of metabolites identification and verification of TCM formula. It is the first systematic study on metabolism of DZSMC in vivo and could also provide a valid analytical strategy for characterization of the chemical compounds and metabolites of TCM formula.
Asunto(s)
Medicamentos Herbarios Chinos/metabolismo , Animales , Apigenina/sangre , Ácidos Cafeicos/sangre , Cromatografía Líquida de Alta Presión , Glucuronatos/sangre , Masculino , Metaboloma , Ratas , Ratas Sprague-Dawley , Espectrometría de Masas en TándemRESUMEN
Poliumoside is representative of phenylethanoid glycosides, which are widely found in many plants. Poliumoside is also regarded as the main active component of Callicarpa kwangtungensis Chun (CK), though its oral bioavailability in rat is extremely low (0.69%) and its in vivo and in vitro metabolism has not yet been systematically investigated. In the present study, an ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) method was employed to identify the metabolites and investigate the metabolic pathways of poliumoside in rat after oral administration 1.5 g·kg-1 of poliumoside. As a result, a total of 34 metabolites (30 from urine, 17 from plasma, and 4 from bile) and 9 possible metabolic pathways (rearrangment, reduction, hydration, hydrolyzation, dehydration, methylation, hydroxylation, acetylation, and sulfation) were proposed in vivo. The main metabolite, acteoside, was quantified after incubated with rat intestinal bacteria in vitro. In conclusion, the present study systematically explored the metabolites of poliumoside in vivo and in vitro, proposing metabolic pathways that may be significant for further metabolic studies of poliumoside.
Asunto(s)
Bacterias/metabolismo , Bilis/química , Ácidos Cafeicos/química , Callicarpa/química , Medicamentos Herbarios Chinos/química , Glicósidos/química , Intestinos/microbiología , Plasma/química , Orina/química , Administración Oral , Animales , Ácidos Cafeicos/administración & dosificación , Ácidos Cafeicos/sangre , Ácidos Cafeicos/orina , Cromatografía Líquida de Alta Presión , Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/metabolismo , Glicósidos/administración & dosificación , Glicósidos/sangre , Glicósidos/orina , Masculino , Espectrometría de Masas/métodos , Estructura Molecular , Ratas , Ratas Sprague-DawleyRESUMEN
A new and sensitive method for fluorescent determination of caffeic acid (CA) was proposed based on silane-functionazed carbon dots coated with molecularly imprinted polymers (CDs@MIPs). CDs@MIPs were prepared by using CA as the templates on the surface of silane-functionazed carbon dots(CDs) with a sol-gel process. The as-prepared CDs@MIPs were characterized by Fourier transform infrared spectroscopy, transmission electron microscopy and fluorescence spectroscopy. The CDs@MIPs exhibited distinguished selectivity and high binding affinity to CA templates, and also showed good reusability. Under optimal conditions, the fluorescence intensity of CDs@MIPs decreased linearly with the increase of CA in the range of 0.5-200⯵M. The limit of detection was 0.11⯵M (3σ/K). Finally, the proposed method was successfully applied to the detection of CA in human plasma. The result indicates that the method built has promising potential for monitoring CA concentration in clinic.
Asunto(s)
Ácidos Cafeicos/sangre , Carbono/química , Colorantes Fluorescentes/química , Impresión Molecular/métodos , Polímeros/química , Puntos Cuánticos/química , Ácidos Cafeicos/análisis , Humanos , Límite de Detección , Puntos Cuánticos/ultraestructura , Espectrometría de Fluorescencia/métodosRESUMEN
Since coffee may help to prevent the development of metabolic syndrome (MetS), we aimed to evaluate the short- and long-term effects of a coffee-based supplement on different features of diet-induced MetS. In this study, 24 Sprague Dawley rats were divided into control or nutraceuticals groups to receive a high-fat/high-fructose diet with or without a mixture of caffeic acid (30 mg/day), trigonelline (20 mg/day), and cafestol (1 mg/day) for 12 weeks. An additional 11 rats were assigned to an acute crossover study. In the chronic experiment, nutraceuticals did not alter body weight or glycemic control, but improved fed hyperinsulinemia (mean difference = 30.80 mU/L, p = 0.044) and homeostatic model assessment-insulin resistance (HOMA-IR) (mean difference = 15.29, p = 0.033), and plasma adiponectin levels (mean difference = -0.99 µg/mL, p = 0.048). The impact of nutraceuticals on post-prandial glycemia tended to be more pronounced after acute administration than at the end of the chronic study. Circulating (mean difference = 4.75 U/L, p = 0.014) and intrahepatocellular alanine transaminase activity was assessed by hyperpolarized-13C nuclear magnetic resonance NMR spectroscopy and found to be reduced by coffee nutraceuticals at endpoint. There was also a tendency towards lower liver triglyceride content and histological steatosis score in the intervention group. In conclusion, a mixture of coffee nutraceuticals improved insulin sensitivity and exhibited hepatoprotective effects in a rat model of MetS. Higher dosages with or without caffeine deserve to be studied in the future.
Asunto(s)
Alcaloides/farmacología , Ácidos Cafeicos/farmacología , Coffea , Dieta Alta en Grasa , Sacarosa en la Dieta , Suplementos Dietéticos , Diterpenos/farmacología , Resistencia a la Insulina , Hígado/efectos de los fármacos , Síndrome Metabólico/prevención & control , Enfermedad del Hígado Graso no Alcohólico/prevención & control , Alcaloides/sangre , Alcaloides/aislamiento & purificación , Animales , Glucemia/efectos de los fármacos , Glucemia/metabolismo , Ácidos Cafeicos/sangre , Ácidos Cafeicos/aislamiento & purificación , Espectroscopía de Resonancia Magnética con Carbono-13 , Coffea/química , Citocinas/sangre , Modelos Animales de Enfermedad , Diterpenos/sangre , Diterpenos/aislamiento & purificación , Insulina/sangre , Lípidos/sangre , Hígado/metabolismo , Hígado/patología , Síndrome Metabólico/sangre , Síndrome Metabólico/etiología , Síndrome Metabólico/patología , Enfermedad del Hígado Graso no Alcohólico/sangre , Enfermedad del Hígado Graso no Alcohólico/etiología , Enfermedad del Hígado Graso no Alcohólico/patología , Ratas Sprague-Dawley , Semillas , Factores de TiempoRESUMEN
Salvianolic acid A (SAA), a water-soluble phenolic acid isolated from the root of Dan Shen, displays distinct antioxidant activity and effectiveness in protection against cerebral ischemia/reperfusion (I/R) damage. However, whether SAA can enter the central nervous system and exert its protective effects by directly targeting brain tissue remains unclear. In this study, we evaluated the cerebral protection of SAA in rats subjected to transient middle cerebral artery occlusion (tMCAO) followed by reperfusion. The rats were treated with SAA (5, 10 mg/kg, iv) when the reperfusion was performed. SAA administration significantly decreased cerebral infarct area and the brain water content, attenuated the neurological deficit and pathology, and enhanced the anti-inflammatory and antioxidant capacity in tMCAO rats. The concentration of SAA in the plasma and brain was detected using LC-MS/MS. A pharmacokinetic study revealed that the circulatory system exposure to SAA was equivalent in the sham controls and I/R rats, but the brain exposure to SAA was significantly higher in the I/R rats than in the sham controls (fold change of 9.17), suggesting that the enhanced exposure to SAA contributed to its cerebral protective effect. Using a GC/MS-based metabolomic platform, metabolites in the serum and brain tissue were extracted and profiled. According to the metabolomic pattern of the tissue data, SAA administration significantly modulated the I/R-caused perturbation of metabolism in the brain to a greater extent than that in the serum, demonstrating that SAA worked at the brain tissue level rather than the whole circulation system. In conclusion, a larger amount of SAA enters the central nervous system in ischemia/reperfusion rats to facilitate its protective and regulatory effects on the perturbed metabolism.
Asunto(s)
Encéfalo/efectos de los fármacos , Ácidos Cafeicos/farmacocinética , Infarto de la Arteria Cerebral Media/tratamiento farmacológico , Lactatos/farmacocinética , Metabolómica/métodos , Fármacos Neuroprotectores/farmacocinética , Daño por Reperfusión/prevención & control , Animales , Disponibilidad Biológica , Encéfalo/metabolismo , Encéfalo/patología , Ácidos Cafeicos/administración & dosificación , Ácidos Cafeicos/sangre , Cromatografía Liquida , Citoprotección , Modelos Animales de Enfermedad , Cromatografía de Gases y Espectrometría de Masas , Infarto de la Arteria Cerebral Media/sangre , Infarto de la Arteria Cerebral Media/patología , Inyecciones Intravenosas , Lactatos/administración & dosificación , Lactatos/sangre , Masculino , Fármacos Neuroprotectores/administración & dosificación , Fármacos Neuroprotectores/sangre , Ratas Sprague-Dawley , Daño por Reperfusión/sangre , Daño por Reperfusión/patología , Espectrometría de Masas en TándemRESUMEN
In this study, a rapid and reliable ultra-fast liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of eight active ingredients, including astragaloside IV, ononin, tanshinol, protocatechualdehyde, protocatechuic acid, salvianolic acid D, rosmarinic acid and ginsenoside Rg1 , in rat plasma. The plasma samples were pretreated by protein precipitation with acetonitrile. Chromatographic separation was performed on a Waters Acquity UPLC® BEH C18 column (1.7 µm particles, 2.1 × 100 mm). The mobile phase consisted of 0.1% aqueous formic acid (A)-acetonitrile with 0.1% formic acid (B) at a flow rate of 0.4 mL/min. Quantification was performed on a triple quadruple tandem mass spectrometry with electrospray ionization by multiple reaction monitoring both in the negative and in the positive ion mode. The lower limit of quantification of tanshinol was 2.0 ng/mL and the others were 5.0 ng/mL. The extraction recoveries, matrix effects, intra- and inter-day precision and accuracy of eight tested components were all within acceptable limits. The validated method was successfully applied to the pharmacokinetic study of the eight active constituents after intragastric administration of three doses (1.0, 3.0, 6.0 g/kg body weight) of Qishen Yiqi Dripping Pills to rats.
Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacocinética , Espectrometría de Masas en Tándem/métodos , Alquenos/análisis , Alquenos/sangre , Animales , Benzaldehídos/análisis , Benzaldehídos/sangre , Ácidos Cafeicos/análisis , Ácidos Cafeicos/sangre , Catecoles/análisis , Catecoles/sangre , Cinamatos/análisis , Cinamatos/sangre , Depsidos/análisis , Depsidos/sangre , Ginsenósidos/análisis , Ginsenósidos/sangre , Glucósidos/análisis , Glucósidos/sangre , Hidroxibenzoatos/análisis , Hidroxibenzoatos/sangre , Isoflavonas/análisis , Isoflavonas/sangre , Límite de Detección , Masculino , Polifenoles/análisis , Polifenoles/sangre , Ratas , Ratas Sprague-Dawley , Saponinas/análisis , Saponinas/sangre , Triterpenos/análisis , Triterpenos/sangre , Ácido RosmarínicoRESUMEN
Dantonic pill, consisting of Salviae miltiorrhize, Panax notoginseng and Borneol, is a widely used compound Chinese medicine for preventing and treating ischemic cardiovascular diseases in China. In the present study, an original and sensitive method for simultaneous determination of tanshinol (i.e. danshensu), protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 in rat plasma by liquid chromatography-tandem mass spectrometry operated in positive/negative ion switching mode was established and validated. The lower limits of quantification for tanshinol, protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 were 5, 0.5, 1, 0.5, 0.5 and 2 ng/mL, respectively. All of the calibration curves showed good linearity over the investigated concentration range (r > 0.99). Validation results demonstrated that the above compounds were accurately, precisely and robustly quantified in rat plasma. The method was successfully applied to characterize the pharmacokinetic profiles of all six compounds in rats following a single oral administration of Dantonic pill.
Asunto(s)
Benzaldehídos/sangre , Ácidos Cafeicos/sangre , Catecoles/sangre , Cromatografía Liquida/métodos , Ginsenósidos/sangre , Hidroxibenzoatos/sangre , Espectrometría de Masas en Tándem/métodos , Animales , Calibración , Límite de Detección , Ratas , Estándares de Referencia , Reproducibilidad de los ResultadosRESUMEN
PURPOSE: Yerba maté is widely consumed in South America as different beverages, such as maté tea (roasted leaves) and chimarrão (green dried leaves), and linked to health benefits, mainly attributed to chlorogenic acids (CGAs). Health effects of CGAs depend on their bioavailability, but such data are scarce. The aim of this study was to investigate the distribution of CGAs and metabolites in tissues, hepatic and plasmatic kinetic profile and urinary excretion after ingestion of maté tea or 5-caffeoylquinic acid (5-CQA). METHODS: Wistar rats ingested maté tea (MT) or 5-CQA (ST) and were killed after 1.5 h for tissue distribution analysis (pilot study) or at 0.5, 1, 2, 4 and 8 h for liver and plasma kinetics (main experiment). Urine was collected in metabolic cages. Biological samples were analyzed by UPLC-DAD-MS with and without incubation with ß-glucuronidase and sulfatase. RESULTS: CGAs and metabolites were detected in all tissues. Caffeic acid was the main compound in plasma up to 2 h after ingestion of maté tea, while 5-CQA predominated in ST group. Concentration of microbial metabolites increased 4 h after gavage and reached higher amounts in MT plasma and liver, when compared to ST group. Approximately 4.0 % of compounds ingested by MT and 3.3 % by ST were recovered in urine up to 8 h after the gavage. CONCLUSION: The study confirms that not only absorption, but also metabolization of CGAs begins in stomach. There were differences in compounds formed from maté tea or isolated 5-CQA, showing that CGAs profile in food may influence qualitatively and quantitatively the metabolites formed in the body.
Asunto(s)
Ácido Clorogénico/farmacocinética , Ilex paraguariensis/química , Ácido Quínico/análogos & derivados , Tés de Hierbas , Animales , Disponibilidad Biológica , Ácidos Cafeicos/sangre , Ácido Clorogénico/administración & dosificación , Extractos Vegetales/administración & dosificación , Extractos Vegetales/farmacocinética , Hojas de la Planta/química , Polifenoles/administración & dosificación , Polifenoles/farmacocinética , Polifenoles/orina , Ácido Quínico/administración & dosificación , Ácido Quínico/farmacocinética , Ratas , Ratas Wistar , América del SurRESUMEN
A rapid, accurate and robust method was firstly developed using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) assay to quantify kukoamine B, which is a novel drug under clinical development for the treatment of sepsis, in human plasma. Solid-phase extraction (SPE) was used to extract kukoamine B from human plasma. The extracts were separated on a Waters Acquity HSS T3 column (2.1×50mm i.d., 1.8µm) with a gradient elution method, using mobile phases of A (formic acid-water (1:1000, v/v)) and B(formic acid-methanol (1:1000, v/v)). Kukoamine B and internal standard (5-deuterated isotope kukoamine B) were detected under the multiple-reaction monitoring mode by an API 5500 triple quadrupole mass spectrometer with electrospray ionization. The method showed good linearity from 0.100 to 50.0ng/mL according to 1/x2 weighted linear regression analysis. Inter- and intra-batch precision of kukoamine B were less than 15% and the accuracy was within 85-115%. The extraction recoveries and matrix effect of kukoamine B at three concentration levels were consistent. The sensitivity, specificity and stabilities under various conditions were validated. In conclusion, the validation results showed that this method was rapid, accurate, robust and can successfully fulfill the requirement of clinical pharmacokinetic study of kukoamine B mesylate in Chinese healthy subjects.