RESUMEN
In-vitro analysis of venous blood taken from rats irradiated by 300 and 5,000 J/m2 of UV showed no effect on metabolism and, therefore, energy and recovery systems of erythrocytes. Concentrations of 2,3-diphosphoglycerate and reduced glutathione were increased after irradiation by 5,000 J/m2. UV-irradiation at 10,000 J/m2 decreased adenosine triphosphate and phospholipids in blood and impaired the functional stability of erythrocyte membranes. Recovery of the membrane structure in 24 hrs. after irradiation suggests extended photochemical processes in cells and is consistent with the literary data about indirect effects of plasma proteins on the red cell function.
Asunto(s)
Membrana Eritrocítica/metabolismo , Membrana Eritrocítica/efectos de la radiación , Rayos Ultravioleta , Adaptación Fisiológica/fisiología , Adenosina Trifosfatasas/metabolismo , Adenosina Trifosfatasas/efectos de la radiación , Animales , Permeabilidad de la Membrana Celular/efectos de los fármacos , Ácidos Difosfoglicéricos/metabolismo , Ácidos Difosfoglicéricos/efectos de la radiación , Glutatión/metabolismo , Glutatión/efectos de la radiación , Técnicas In Vitro , Fosfolípidos/metabolismo , Fosfolípidos/efectos de la radiación , Dosis de Radiación , Ratas , Ratas Wistar , Factores de TiempoRESUMEN
The effect of total body irradiation (5 Gy) on functional mouse erythroid lineage has been studied. The transferrin binding capacity by bone marrow cells and the activity of glycolytic regulatory enzymes and intracellular levels of 2,3 bisphosphoglycerate in peripheral blood erythrocytes have been determined. Results obtained along one year post-irradiation period suggest a complete recovery in the erythroid cell lineage with respect to the biological endpoints investigated.
Asunto(s)
Médula Ósea/efectos de la radiación , Ácidos Difosfoglicéricos/efectos de la radiación , Eritrocitos/efectos de la radiación , Fosfotransferasas/efectos de la radiación , Receptores de Transferrina/efectos de la radiación , Transferrina/metabolismo , 2,3-Difosfoglicerato , Animales , Células de la Médula Ósea , Ácidos Difosfoglicéricos/sangre , Eritrocitos/enzimología , Hexoquinasa/efectos de la radiación , Ratones , Ratones Endogámicos BALB C , Fosfofructoquinasa-1/efectos de la radiación , Fosfotransferasas/sangre , Piruvato Quinasa/efectos de la radiación , Irradiación Corporal TotalRESUMEN
The authors studied the effects of gamma radiation and submaximal physical exercise on 2,3-DFG content in healthy men erythrocytes. Twelve men aged 20-22 were examined. They were loaded by physical exercise (at doses of 2 M/kg body weight) for 15 minutes. Erythrocytes were exposed to gamma radiation (500 Gy doses) from a 60Co source. The concentration of 2,3-DFG in erythrocytes was estimated by Bartlett's method. Gamma radiation at 500 Gy dose was found to decrease 2,3-DFG content in erythrocytes both at rest and after submaximal exercise. Furthermore, submaximal physical exercise was found to decrease 2,3-DFG content in non-irradiated erythrocytes.