RESUMEN
Unique chemical and physical properties are introduced by inserting selenocysteine (Sec) at specific sites within proteins. Recombinant and facile production of eukaryotic selenoproteins would benefit from a yeast expression system; however, the selenoprotein biosynthetic pathway was lost in the evolution of the kingdom Fungi as it diverged from its eukaryotic relatives. Based on our previous development of efficient selenoprotein production in bacteria, we designed a novel Sec biosynthesis pathway in Saccharomyces cerevisiae using Aeromonas salmonicida translation components. S. cerevisiae tRNASer was mutated to resemble A. salmonicida tRNASec to allow recognition by S. cerevisiae seryl-tRNA synthetase as well as A. salmonicida selenocysteine synthase (SelA) and selenophosphate synthetase (SelD). Expression of these Sec pathway components was then combined with metabolic engineering of yeast to enable the production of active methionine sulfate reductase enzyme containing genetically encoded Sec. Our report is the first demonstration that yeast is capable of selenoprotein production by site-specific incorporation of Sec.
Asunto(s)
Saccharomyces cerevisiae , Codón de Terminación/genética , Codón de Terminación/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Aeromonas salmonicida/genética , Ingeniería de Proteínas , ARN de Transferencia de Cisteína/química , ARN de Transferencia de Cisteína/genética , ARN de Transferencia de Cisteína/metabolismo , Humanos , Conformación de Ácido NucleicoRESUMEN
In Bacillus subtilis, the tRNACys lacks an encoded CCA 3' end. To gain insight into the role of CCAase and RNases in tRNACys processing, several mutant strains were generated. Northern blot and RT-PCR results suggest that enzymes other than CCAase can participate in CCA addition at the 3' end of the immature tRNACys.
Asunto(s)
Bacillus subtilis/enzimología , Proteínas Bacterianas/metabolismo , ARN Nucleotidiltransferasas/deficiencia , ARN Bacteriano/genética , ARN de Transferencia de Cisteína/genética , Bacillus subtilis/química , Bacillus subtilis/genética , Bacillus subtilis/metabolismo , Proteínas Bacterianas/genética , Secuencia de Bases , Conformación de Ácido Nucleico , ARN Nucleotidiltransferasas/genética , Procesamiento Postranscripcional del ARN , ARN Bacteriano/química , ARN Bacteriano/metabolismo , ARN de Transferencia de Cisteína/química , ARN de Transferencia de Cisteína/metabolismoRESUMEN
Selenium (Se) is an essential trace element for several organisms and is mostly present in proteins as L-selenocysteine (Sec or U). Sec is synthesized on its L-seryl-tRNASec to produce Sec-tRNASec molecules by a dedicated selenocysteine synthesis machinery and incorporated into selenoproteins at specified in-frame UGA codons. UGA-Sec insertion is signaled by an mRNA stem-loop structure called the SElenoCysteine Insertion Sequence (SECIS). tRNASec transcription regulation and folding have been described showing its importance to Sec biosynthesis. Here, we discuss structural aspects of Sec-tRNASec and its role in Sec biosynthesis as well as Sec incorporation into selenoproteins. Defects in the Sec biosynthesis or incorporation pathway have been correlated with pathological conditions.
Asunto(s)
ARN de Transferencia de Cisteína/genética , Selenocisteína/biosíntesis , Animales , Codón de Terminación/química , Codón de Terminación/genética , Codón de Terminación/metabolismo , Humanos , Biosíntesis de Proteínas , ARN Mensajero/química , ARN Mensajero/genética , ARN Mensajero/metabolismo , ARN de Transferencia de Cisteína/química , ARN de Transferencia de Cisteína/metabolismo , Selenocisteína/genéticaRESUMEN
Cysteine can be synthesized by tRNA-dependent mechanism using a two-step indirect pathway, where O-phosphoseryl-tRNA synthetase (SepRS) catalyzes the ligation of a mismatching O-phosphoserine (Sep) to tRNACys followed by the conversion of tRNA-bounded Sep into cysteine by Sep-tRNA:Cys-tRNA synthase (SepCysS). In ancestral methanogens, a third protein SepCysE forms a bridge between the two enzymes to create a ternary complex named the transsulfursome. By combination of X-ray crystallography, SAXS and EM, together with biochemical evidences, here we show that the three domains of SepCysE each bind SepRS, SepCysS, and tRNACys, respectively, which mediates the dynamic architecture of the transsulfursome and thus enables a global long-range channeling of tRNACys between SepRS and SepCysS distant active sites. This channeling mechanism could facilitate the consecutive reactions of the two-step indirect pathway of Cys-tRNACys synthesis (tRNA-dependent cysteine biosynthesis) to prevent challenge of translational fidelity, and may reflect the mechanism that cysteine was originally added into genetic code.
Asunto(s)
Aminoacil-ARNt Sintetasas/metabolismo , Proteínas Arqueales/metabolismo , Cisteína/metabolismo , ARN de Transferencia de Cisteína/metabolismo , Aminoacil-ARNt Sintetasas/química , Aminoacil-ARNt Sintetasas/genética , Proteínas Arqueales/química , Proteínas Arqueales/genética , Cristalografía por Rayos X , Cisteína/química , Cisteína/genética , Código Genético/genética , Methanocaldococcus/genética , Methanocaldococcus/metabolismo , Microscopía Electrónica , Modelos Moleculares , Mutación , Fosfoserina/química , Fosfoserina/metabolismo , Unión Proteica , Conformación Proteica , ARN de Transferencia de Cisteína/química , ARN de Transferencia de Cisteína/genética , Dispersión del Ángulo PequeñoRESUMEN
RNase P is a universal enzyme that removes 5' leader sequences from tRNA precursors. The enzyme is therefore essential for maturation of functional tRNAs and mRNA translation. RNase P represents a unique example of an enzyme that can occur either as ribonucleoprotein or as protein alone. The latter form of the enzyme, called protein-only RNase P (PRORP), is widespread in eukaryotes in which it can provide organellar or nuclear RNase P activities. Here, we have focused on Arabidopsis nuclear PRORP2 and its interaction with tRNA substrates. Affinity measurements helped assess the respective importance of individual pentatricopeptide repeat motifs in PRORP2 for RNA binding. We characterized the PRORP2 structure by X-ray crystallography and by small-angle X-ray scattering in solution as well as that of its complex with a tRNA precursor by small-angle X-ray scattering. Of note, our study reports the first structural data of a PRORP-tRNA complex. Combined with complementary biochemical and biophysical analyses, our structural data suggest that PRORP2 undergoes conformational changes to accommodate its substrate. In particular, the catalytic domain and the RNA-binding domain can move around a central hinge. Altogether, this work provides a refined model of the PRORP-tRNA complex that illustrates how protein-only RNase P enzymes specifically bind tRNA and highlights the contribution of protein dynamics to achieve this specific interaction.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Modelos Moleculares , Precursores del ARN/metabolismo , Procesamiento Postranscripcional del ARN , ARN de Planta/metabolismo , ARN de Transferencia de Cisteína/metabolismo , Ribonucleasa P/metabolismo , Secuencias de Aminoácidos , Sustitución de Aminoácidos , Arabidopsis/enzimología , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Fenómenos Biofísicos , Dominio Catalítico , Estabilidad de Enzimas , Mutación , Conformación de Ácido Nucleico , Motivos de Nucleótidos , Fragmentos de Péptidos/química , Fragmentos de Péptidos/genética , Fragmentos de Péptidos/metabolismo , Conformación Proteica , Dominios y Motivos de Interacción de Proteínas , ARN/química , ARN/metabolismo , Precursores del ARN/química , ARN de Planta/química , ARN de Transferencia de Cisteína/química , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Ribonucleasa P/química , Ribonucleasa P/genética , SolubilidadRESUMEN
Oxidative stress occurs when cells are exposed to elevated levels of reactive oxygen species that can damage biological molecules. One bacterial response to oxidative stress involves disulfide bond formation either between protein thiols or between protein thiols and low-molecular-weight (LMW) thiols. Bacillithiol was recently identified as a major low-molecular-weight thiol in Bacillus subtilis and related Firmicutes. Four genes (bshA, bshB1, bshB2, and bshC) are involved in bacillithiol biosynthesis. The bshA and bshB1 genes are part of a seven-gene operon (ypjD), which includes the essential gene cca, encoding CCA-tRNA nucleotidyltransferase. The inclusion of cca in the operon containing bacillithiol biosynthetic genes suggests that the integrity of the 3' terminus of tRNAs may also be important in oxidative stress. The addition of the 3' terminal CCA sequence by CCA-tRNA nucleotidyltransferase to give rise to a mature tRNA and functional molecules ready for aminoacylation plays an essential role during translation and expression of the genetic code. Any defects in these processes, such as the accumulation of shorter and defective tRNAs under oxidative stress, might exert a deleterious effect on cells. This review summarizes the physiological link between tRNACys regulation and oxidative stress in Bacillus.
Asunto(s)
Bacillus subtilis/genética , ARN Nucleotidiltransferasas/metabolismo , ARN de Transferencia de Cisteína/metabolismo , Bacillus subtilis/metabolismo , Proteínas Bacterianas/metabolismo , Cisteína/análogos & derivados , Cisteína/biosíntesis , Disulfuros/metabolismo , Glucosamina/análogos & derivados , Glucosamina/biosíntesis , Modelos Moleculares , Estrés Oxidativo , ARN Bacteriano/metabolismo , ARN de Transferencia de Cisteína/químicaRESUMEN
We report the identification of novel tRNA species with 12-base pair amino-acid acceptor branches composed of longer acceptor stem and shorter T-stem. While canonical tRNAs have a 7/5 configuration of the branch, the novel tRNAs have either 8/4 or 9/3 structure. They were found during the search for selenocysteine tRNAs in terabytes of genome, metagenome and metatranscriptome sequences. Certain bacteria and their phages employ the 8/4 structure for serine and histidine tRNAs, while minor cysteine and selenocysteine tRNA species may have a modified 8/4 structure with one bulge nucleotide. In Acidobacteria, tRNAs with 8/4 and 9/3 structures may function as missense and nonsense suppressor tRNAs and/or regulatory noncoding RNAs. In δ-proteobacteria, an additional cysteine tRNA with an 8/4 structure mimics selenocysteine tRNA and may function as opal suppressor. We examined the potential translation function of suppressor tRNA species in Escherichia coli; tRNAs with 8/4 or 9/3 structures efficiently inserted serine, alanine and cysteine in response to stop and sense codons, depending on the identity element and anticodon sequence of the tRNA. These findings expand our view of how tRNA, and possibly the genetic code, is diversified in nature.
Asunto(s)
ARN Bacteriano/química , ARN de Transferencia/química , Anticodón , Bacterias/genética , Toxinas Bacterianas/genética , Conformación de Ácido Nucleico , Biosíntesis de Proteínas , ARN de Transferencia Aminoácido-Específico/química , ARN de Transferencia de Cisteína/química , ARN de Transferencia de Cisteína/metabolismoRESUMEN
In most organisms, Cys-tRNA(Cys) is directly synthesized by cysteinyl-tRNA synthetase (CysRS). Many methanogenic archaea, however, use a two-step, indirect pathway to synthesize Cys-tRNA(Cys) owing to a lack of CysRS and cysteine-biosynthesis systems. This reaction is catalyzed by O-phosphoseryl-tRNA synthetase (SepRS), Sep-tRNA:Cys-tRNA synthase (SepCysS) and SepRS/SepCysS pathway enhancer (SepCysE) as the transsulfursome, in which SepCysE connects both SepRS and SepCysS. On the transsulfursome, SepRS first ligates an O-phosphoserine to tRNA(Cys), and the mischarged intermediate Sep-tRNA(Cys) is then transferred to SepCysS, where it is further modified to Cys-tRNA(Cys). In this study, a subcomplex of the transsulfursome with tRNA(Cys) (SepCysS-SepCysE-tRNA(Cys)), which is involved in the second reaction step of the indirect pathway, was constructed and then crystallized. The crystals diffracted X-rays to a resolution of 2.6â Å and belonged to space group P6522, with unit-cell parameters a = b = 107.2, c = 551.1â Å. The structure determined by molecular replacement showed that the complex consists of a SepCysS dimer, a SepCysE dimer and one tRNA(Cys) in the asymmetric unit.
Asunto(s)
Aminoacil-ARNt Sintetasas/química , Proteínas Arqueales/química , Methanocaldococcus/química , ARN de Transferencia de Cisteína/química , Secuencia de Aminoácidos , Aminoacil-ARNt Sintetasas/genética , Aminoacil-ARNt Sintetasas/metabolismo , Proteínas Arqueales/genética , Proteínas Arqueales/metabolismo , Clonación Molecular , Cristalización , Cristalografía por Rayos X , Escherichia coli/genética , Escherichia coli/metabolismo , Expresión Génica , Methanocaldococcus/enzimología , Plásmidos/química , Plásmidos/metabolismo , ARN de Transferencia de Cisteína/genética , ARN de Transferencia de Cisteína/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Difracción de Rayos XRESUMEN
In vitro-transcribed suppressor tRNAs are commonly used in site-specific fluorescence labeling for protein and ribosome-bound nascent chains (RNCs) studies. Here, we describe the production of nonorthogonal Bacillus subtilis tRNA(cys)(Amber) from Escherichia coli, a process that is superior to in vitro transcription in terms of yield, ease of manipulation, and tRNA stability. As cysteinyl-tRNA synthetase was previously shown to aminoacylate tRNA(cys)(Amber) with lower efficiency, multiple tRNA synthetase mutants were designed to optimize aminoacylation. Aminoacylated tRNA was conjugated to a fluorophore to produce BODIPY FL-cysteinyl-tRNA(cys)(Amber), which was used to generate ribosome-bound nascent chains of different lengths with the fluorophore incorporated at various predetermined sites. This tRNA tool may be beneficial in the site-specific labeling of full-length proteins as well as RNCs for biophysical and biological research.
Asunto(s)
Aminoacil-ARNt Sintetasas/metabolismo , Bacillus subtilis/genética , Escherichia coli/genética , ARN de Transferencia de Cisteína/biosíntesis , ARN de Transferencia de Cisteína/química , Aminoacil-ARNt Sintetasas/genética , Sistema Libre de Células , Escherichia coli/metabolismo , Colorantes Fluorescentes/química , Técnicas In Vitro , Modelos Moleculares , Biosíntesis de Proteínas , Estabilidad del ARN , ARN Bacteriano/biosíntesis , ARN Bacteriano/química , ARN Bacteriano/genética , ARN de Transferencia de Cisteína/genética , Aminoacilación de ARN de TransferenciaRESUMEN
The putative translation elongation factor Mbar_A0971 from the methanogenic archaeon Methanosarcina barkeri was proposed to be the pyrrolysine-specific paralogue of EF-Tu ("EF-Pyl"). In the present study, the crystal structures of its homologue from Methanosarcina mazei (MM1309) were determined in the GMPPNP-bound, GDP-bound, and apo forms, by the single-wavelength anomalous dispersion phasing method. The three MM1309 structures are quite similar (r.m.s.d. < 0.1 Å). The three domains, corresponding to domains 1, 2, and 3 of EF-Tu/SelB/aIF2γ, are packed against one another to form a closed architecture. The MM1309 structures resemble those of bacterial/archaeal SelB, bacterial EF-Tu in the GTP-bound form, and archaeal initiation factor aIF2γ, in this order. The GMPPNP and GDP molecules are visible in their co-crystal structures. Isothermal titration calorimetry measurements of MM1309·GTP·Mg(2+), MM1309·GDP·Mg(2+), and MM1309·GMPPNP·Mg(2+) provided dissociation constants of 0.43, 26.2, and 222.2 µM, respectively. Therefore, the affinities of MM1309 for GTP and GDP are similar to those of SelB rather than those of EF-Tu. Furthermore, the switch I and II regions of MM1309 are involved in domain-domain interactions, rather than nucleotide binding. The putative binding pocket for the aminoacyl moiety on MM1309 is too small to accommodate the pyrrolysyl moiety, based on a comparison of the present MM1309 structures with that of the EF-Tu·GMPPNP·aminoacyl-tRNA ternary complex. A hydrolysis protection assay revealed that MM1309 binds cysteinyl (Cys)-tRNA(Cys) and protects the aminoacyl bond from non-enzymatic hydrolysis. Therefore, we propose that MM1309 functions as either a guardian protein that protects the Cys moiety from oxidation or an alternative translation factor for Cys-tRNA(Cys).
Asunto(s)
Proteínas Arqueales/química , Guanosina Trifosfato/química , Methanosarcina/química , ARN de Transferencia de Cisteína/química , Secuencia de Aminoácidos , Proteínas Arqueales/genética , Proteínas Arqueales/metabolismo , Calorimetría , Cristalografía por Rayos X , Guanosina Difosfato/química , Guanosina Difosfato/metabolismo , Guanosina Trifosfato/metabolismo , Guanilil Imidodifosfato/química , Guanilil Imidodifosfato/metabolismo , Cinética , Methanosarcina/genética , Methanosarcina/metabolismo , Modelos Moleculares , Datos de Secuencia Molecular , Estructura Molecular , Conformación de Ácido Nucleico , Factor Tu de Elongación Peptídica/química , Factor Tu de Elongación Peptídica/genética , Factor Tu de Elongación Peptídica/metabolismo , Factores de Elongación de Péptidos/química , Factores de Elongación de Péptidos/genética , Factores de Elongación de Péptidos/metabolismo , Factores de Iniciación de Péptidos/química , Factores de Iniciación de Péptidos/genética , Factores de Iniciación de Péptidos/metabolismo , Unión Proteica , Estructura Terciaria de Proteína , ARN de Transferencia de Cisteína/metabolismo , Homología de Secuencia de AminoácidoRESUMEN
Methanogenic archaea lack cysteinyl-tRNA synthetase; they synthesize Cys-tRNA and cysteine in a tRNA-dependent manner. Two enzymes are required: Phosphoseryl-tRNA synthetase (SepRS) forms phosphoseryl-tRNA(Cys) (Sep-tRNA(Cys)), which is converted to Cys-tRNA(Cys) by Sep-tRNA:Cys-tRNA synthase (SepCysS). This represents the ancestral pathway of Cys biosynthesis and coding in archaea. Here we report a translation factor, SepCysE, essential for methanococcal Cys biosynthesis; its deletion in Methanococcus maripaludis causes Cys auxotrophy. SepCysE acts as a scaffold for SepRS and SepCysS to form a stable high-affinity complex for tRNA(Cys) causing a 14-fold increase in the initial rate of Cys-tRNA(Cys) formation. Based on our crystal structure (2.8-Å resolution) of a SepCysSâ SepCysE complex, a SepRSâ SepCysEâ SepCysS structure model suggests that this ternary complex enables substrate channeling of Sep-tRNA(Cys). A phylogenetic analysis suggests coevolution of SepCysE with SepRS and SepCysS in the last universal common ancestral state. Our findings suggest that the tRNA-dependent Cys biosynthesis proceeds in a multienzyme complex without release of the intermediate and this mechanism may have facilitated the addition of Cys to the genetic code.
Asunto(s)
Archaea/metabolismo , Proteínas Arqueales/metabolismo , Cisteína/biosíntesis , Methanococcus/metabolismo , Factores de Iniciación de Péptidos/metabolismo , ARN de Transferencia de Cisteína/metabolismo , Acetilación , Proteínas Arqueales/química , Secuencia Conservada , Cristalografía por Rayos X , Cinética , Modelos Moleculares , Factores de Iniciación de Péptidos/química , Unión Proteica , Estructura Terciaria de Proteína , ARN de Transferencia de Cisteína/químicaRESUMEN
Protein structure networks are constructed for the identification of long-range signaling pathways in cysteinyl tRNA synthetase (CysRS). Molecular dynamics simulation trajectory of CysRS-ligand complexes were used to determine conformational ensembles in order to gain insight into the allosteric signaling paths. Communication paths between the anticodon binding region and the aminoacylation region have been identified. Extensive interaction between the helix bundle domain and the anticodon binding domain, resulting in structural rigidity in the presence of tRNA, has been detected. Based on the predicted model, six residues along the communication paths have been examined by mutations (single and double) and shown to mediate a coordinated coupling between anticodon recognition and activation of amino acid at the active site. This study on CysRS clearly shows that specific key residues, which are involved in communication between distal sites in allosteric proteins but may be elusive in direct structure analysis, can be identified from dynamics of protein structure networks.
Asunto(s)
Aminoacil-ARNt Sintetasas/metabolismo , Anticodón/metabolismo , Proteínas de Escherichia coli/metabolismo , Escherichia coli/enzimología , ARN Bacteriano/metabolismo , ARN de Transferencia de Cisteína/metabolismo , Regulación Alostérica/fisiología , Aminoacil-ARNt Sintetasas/química , Aminoacil-ARNt Sintetasas/genética , Anticodón/química , Anticodón/genética , Escherichia coli/genética , Proteínas de Escherichia coli/química , Proteínas de Escherichia coli/genética , Mutación , Conformación Proteica , ARN Bacteriano/química , ARN Bacteriano/genética , ARN de Transferencia de Cisteína/química , ARN de Transferencia de Cisteína/genéticaRESUMEN
Although the nucleotides in tRNA required for aminoacylation are conserved in evolution, bacterial aminoacyl-transfer RNA synthetases are unable to acylate eukaryotic tRNA. The cross-species barrier may be due to the absence of eukaryote-specific domains from bacterial aminoacyl-transfer RNA synthetases. Here we show that whereas Escherichia coli CysRS cannot acylate human tRNA(Cys), the fusion of a eukaryote-specific domain of human CysRS overcomes the cross-species barrier in human tRNA(Cys). In addition to enabling recognition of the sequence differences in the tertiary core of tRNA(Cys), the fused eukaryotic domain redirects the specificity of E. coli CysRS from the A37 present in bacterial tRNA(Cys) to the G37 in mammals. Further experiments show that the accuracy of codon recognition on the ribosome was also highly sensitive to the A37G transition in tRNA(Cys). These results raise the possibility of the development of tRNA nucleotide determinants for aminoacylation being interdependent with those for ribosome decoding.
Asunto(s)
Aminoacil-ARNt Sintetasas/fisiología , Proteínas de Escherichia coli/fisiología , Evolución Molecular , ARN de Transferencia de Cisteína/metabolismo , ARN de Transferencia/química , Ribosomas/fisiología , Aminoacilación de ARN de Transferencia , Aminoacil-ARNt Sintetasas/química , Secuencia de Bases , Proteínas de Escherichia coli/química , Inestabilidad Genómica , Humanos , Cinética , Conformación de Ácido Nucleico , ARN de Transferencia de Cisteína/química , Especificidad de la EspecieRESUMEN
The modified nucleosides N(2)-methylguanosine and N(2)(2)-dimethylguanosine in transfer RNA occur at five positions in the D and anticodon arms, and at positions G6 and G7 in the acceptor stem. Trm1 and Trm11 enzymes are known to be responsible for several of the D/anticodon arm modifications, but methylases catalyzing post-transcriptional m(2)G synthesis in the acceptor stem are uncharacterized. Here, we report that the MJ0438 gene from Methanocaldococcus jannaschii encodes a novel S-adenosylmethionine-dependent methyltransferase, now identified as Trm14, which generates m(2)G at position 6 in tRNA(Cys). The 381 amino acid Trm14 protein possesses a canonical RNA recognition THUMP domain at the amino terminus, followed by a γ-class Rossmann fold amino-methyltransferase catalytic domain featuring the signature NPPY active site motif. Trm14 is associated with cluster of orthologous groups (COG) 0116, and most closely resembles the m(2)G10 tRNA methylase Trm11. Phylogenetic analysis reveals a canonical archaeal/bacterial evolutionary separation with 20-30% sequence identities between the two branches, but it is likely that the detailed functions of COG 0116 enzymes differ between the archaeal and bacterial domains. In the archaeal branch, the protein is found exclusively in thermophiles. More distantly related Trm14 homologs were also identified in eukaryotes known to possess the m(2)G6 tRNA modification.
Asunto(s)
Proteínas Arqueales/metabolismo , Methanococcales/enzimología , ARN de Transferencia/metabolismo , ARNt Metiltransferasas/metabolismo , Secuencia de Aminoácidos , Proteínas Arqueales/clasificación , Proteínas Arqueales/genética , Secuencia de Bases , Biocatálisis , Datos de Secuencia Molecular , Filogenia , ARN de Transferencia/química , ARN de Transferencia de Cisteína/química , ARN de Transferencia de Cisteína/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Alineación de Secuencia , ARNt Metiltransferasas/clasificación , ARNt Metiltransferasas/genéticaRESUMEN
We generated a conditional CCase mutant of Bacillus subtilis to explore the participation in vivo of the tRNA nucleotidyltransferase (CCA transferase or CCase) in the maturation of the single-copy tRNA(Cys), which lacks an encoded CCA 3' end. We observed that shorter tRNA(Cys) species, presumably lacking CCA, only accumulated when the inducible Pspac : cca was introduced into an rnr mutant strain, but not in combination with pnp. We sequenced the tRNA 3' ends produced in the various mutant tRNA(Cys) species to detect maturation and decay intermediates and observed that decay of the tRNA(Cys) occurs through the addition of poly(A) or heteropolymeric tails. A few clones corresponding to full-size tRNAs contained either CCA or other C and/or A sequences, suggesting that these are substrates for repair and/or decay. We also observed editing of tRNA(Cys) at position 21, which seems to occur preferentially in mature tRNAs. Altogether, our results provide in vivo evidence for the participation of the B. subtilis cca gene product in the maturation of tRNAs lacking CCA. We also suggest that RNase R exoRNase in B. subtilis participates in the quality control of tRNA.
Asunto(s)
Bacillus subtilis/metabolismo , Proteínas Bacterianas/metabolismo , Exorribonucleasas/metabolismo , Mutación , ARN Nucleotidiltransferasas/genética , Procesamiento Postranscripcional del ARN , ARN de Transferencia de Cisteína/metabolismo , Bacillus subtilis/química , Bacillus subtilis/enzimología , Bacillus subtilis/genética , Proteínas Bacterianas/genética , Secuencia de Bases , Exorribonucleasas/genética , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , ARN Nucleotidiltransferasas/metabolismo , ARN de Transferencia de Cisteína/química , ARN de Transferencia de Cisteína/genéticaRESUMEN
Strict one-to-one correspondence between codons and amino acids is thought to be an essential feature of the genetic code. However, we report that one codon can code for two different amino acids with the choice of the inserted amino acid determined by a specific 3' untranslated region structure and location of the dual-function codon within the messenger RNA (mRNA). We found that the codon UGA specifies insertion of selenocysteine and cysteine in the ciliate Euplotes crassus, that the dual use of this codon can occur even within the same gene, and that the structural arrangements of Euplotes mRNA preserve location-dependent dual function of UGA when expressed in mammalian cells. Thus, the genetic code supports the use of one codon to code for multiple amino acids.
Asunto(s)
Codón de Terminación/genética , Codón/genética , Cisteína/genética , Euplotes/genética , Código Genético , Selenocisteína/genética , Selenoproteínas/genética , Regiones no Traducidas 3' , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Línea Celular , Cisteína/metabolismo , Euplotes/química , Humanos , Datos de Secuencia Molecular , Mutación , Proteínas Protozoarias/biosíntesis , Proteínas Protozoarias/química , Proteínas Protozoarias/genética , ARN Protozoario/genética , ARN Protozoario/metabolismo , ARN de Transferencia Aminoácido-Específico/química , ARN de Transferencia Aminoácido-Específico/genética , ARN de Transferencia de Cisteína/química , ARN de Transferencia de Cisteína/genética , Proteínas Recombinantes de Fusión/metabolismo , Selenocisteína/metabolismo , Selenoproteínas/biosíntesis , Selenoproteínas/químicaRESUMEN
All mature tRNA molecules have the conserved CCA sequence at the 3' end with a range of dynamic conformations that are important for tRNA functions. We present here the details of a general approach to fluorescent labeling of the CCA sequence with the fluorescent base analog pyrrolo-C (PyC) at position 75 as a molecular probe for monitoring the dynamics of the tRNA 3' end. Using Escherichia coli tRNA(Cys) as an example, we achieve such labeling by first synthesizing the tRNA as a transcript up to C74 and then employing the tRNA CCA-adding enzyme to incorporate PyC75 and A76, using pyrrolo-CTP (PyCTP) and ATP as the respective substrates. PyC-labeled full-length tRNA(Cys), separated from the unlabeled precursor tRNA by reverse phase high-pressure liquid chromatography, is an efficient substrate for aminoacylation by E. coli cysteinyl-tRNA synthetase (CysRS). Fluorescence binding measurement of the PyC-labeled tRNA(Cys) with E. coli CysRS reveals an equilibrium K(d) closely similar to the value determined from the fluorescence of intrinsic enzyme tryptophans. Kinetic measurements of translocation of the PyC-labeled tRNA from the ribosomal A to P sites identify a kinetic intermediate with a rate of formation and decay similar to the values reported for tRNAs labeled with the fluorescent proflavin at the tertiary core. These results highlight the potential of PyC to probe the dynamics of the tRNA CCA end in reactions ranging from aminoacylation to those on the ribosome.
Asunto(s)
Citosina/análogos & derivados , Colorantes Fluorescentes/química , Conformación de Ácido Nucleico , Pirroles/química , Sondas ARN , ARN de Transferencia/química , Aminoacilación , Citidina/análogos & derivados , Citosina/química , Escherichia coli/genética , ARN Nucleotidiltransferasas/química , ARN Bacteriano/química , ARN de Transferencia de Cisteína/química , Ribosomas/metabolismoRESUMEN
We report here on the clinical, genetic, and molecular characterization of three Han Chinese pedigrees with aminoglycoside-induced and nonsyndromic hearing loss. Clinical evaluation revealed the variable phenotype of hearing impairment including severity, age-at-onset, audiometric configuration in these subjects. The penetrance of hearing loss in WZD8, WZD9, and WZD10 pedigrees were 46%, 46%, and 50%, respectively, when aminoglycoside-induced deafness was included. When the effect of aminoglycosides was excluded, the penetrance of hearing loss in these pedigrees were 23%, 31%, and 37.5%, respectively. Mutational analysis of the complete mitochondrial genomes showed the homoplasmic A1555G mutation and distinct sets of mitochondrial DNA variants belonging to haplogroups D4b2b, B5b1, and F2, respectively. Of these, the tRNA(Cys) T5802C, tRNA(Thr) A15924C, and ND5 T12338C variants are of special interest as these variants occur at positions which are highly evolutionarily conserved nucleotides of tRNAs or amino acid of polypeptide. These homoplasmic mtDNA variants were absent among 156 unrelated Chinese controls. The T5802C and G15927A variants disrupted a highly conserved A-U or C-G base-pairing at the anticodon-stem of tRNA(Cys) or tRNA(Thr), while the ND5 T12338C mutation resulted in the replacement of the translation-initiating methionine with a threonine, and also located in two nucleotides adjacent to the 3' end of the tRNA(Leu(CUN)). Thus, mitochondrial dysfunctions, caused by the A1555G mutation, would be worsened by these mtDNA variants. Therefore, these mtDNA mutations may have a potential modifier role in increasing the penetrance and expressivity of the deafness-associated 12S rRNA A1555G mutation in those Chinese pedigrees.
Asunto(s)
ADN Mitocondrial/genética , Sordera/genética , Mutación , Linaje , ARN Ribosómico/genética , Aminoglicósidos/farmacología , Secuencia de Bases , China , Conexina 26 , Conexinas/genética , Sordera/inducido químicamente , Sordera/fisiopatología , Variación Genética , Haplotipos , Humanos , Proteínas Mitocondriales/genética , Datos de Secuencia Molecular , Penetrancia , Fenotipo , ARN de Transferencia de Cisteína/química , ARN de Transferencia de Cisteína/genética , ARN de Transferencia de Treonina/química , ARN de Transferencia de Treonina/genética , Índice de Severidad de la Enfermedad , ARNt Metiltransferasas/genéticaRESUMEN
In nature, ribosomally synthesized proteins can contain at least 22 different amino acids: the 20 common amino acids as well as selenocysteine and pyrrolysine. Each of these amino acids is inserted into proteins codon-specifically via an aminoacyl-transfer RNA (aa-tRNA). In most cases, these aa-tRNAs are biosynthesized directly by a set of highly specific and accurate aminoacyl-tRNA synthetases (aaRSs). However, in some cases aaRSs with relaxed or novel substrate specificities cooperate with other enzymes to generate specific canonical and non-canonical aminoacyl-tRNAs.
Asunto(s)
Aminoacilación de ARN de Transferencia , Aminoacil-ARNt Sintetasas/metabolismo , Aspartato-ARNt Ligasa/metabolismo , Bacterias/enzimología , Aminoacil-ARN de Transferencia/biosíntesis , Aminoacil-ARN de Transferencia/química , Aminoacil-ARN de Transferencia/metabolismo , ARN de Transferencia de Asparagina/biosíntesis , ARN de Transferencia de Asparagina/química , ARN de Transferencia de Cisteína/biosíntesis , ARN de Transferencia de Cisteína/química , ARN de Transferencia de Glutamina/biosíntesis , ARN de Transferencia de Glutamina/químicaRESUMEN
Prolyl-tRNA synthetases (ProRSs) are unique among synthetases in that they have diverse architectures, notably the variable presence of a cis-editing domain homologous to the freestanding deacylase proteins YbaK and ProX. Here, we describe crystal structures of two bacterial ProRSs from the pathogen Enterococcus faecalis, which possesses an editing domain, and from Rhodopseudomonas palustris, which does not. We compare the overall structure and binding mode of ATP and prolyl-adenylate with those of the archael/eukaryote-type ProRS from Thermus thermophilus. Although structurally more homologous to YbaK, which preferentially hydrolyzes Cys-tRNA(Pro), the editing domain of E. faecalis ProRS possesses key elements similar to ProX, with which it shares the activity of hydrolyzing Ala-tRNA(Pro). The structures give insight into the complex evolution of ProRSs, the mechanism of editing, and structural differences between prokaryotic- and eukaryotic-type ProRSs that can be exploited for antibiotic design.