Actinobacillosis outbreak in cattle with clinical manifestation of hippopotamus-like face / Actinobacilose em bovinos com manifestação clínica de cara de hipopótamo

Actinobacillosis outbreak with clinical manifestation of hippopotamus-like face observed in a property located in the municipality of Capão do Leão, Southern Brazil, in September 2016, is described. The cattle herd remained for most of the year in rice stubble. When these areas were occupied with new crops, they were transferred to areas where there were small native forests. Three cattle were affected. They presented a volume increase in the nasolabial and maxillary region, and there was also regional lymph node swelling. The evolution of the disease occurred in approximately six months. In tissue fragments collected for culture, Actinobacillus lignieresii was isolated. The diagnosis was based on clinical findings, histopathological evaluation characterized by the presence of piogranulomas with Splendore Hoepli reaction in its center, bacterial isolation, and identification of A. lignieresii by polymerase chain reaction (PRC) and genetic sequencing.(AU)

Descreve-se um surto de actinobacilose com manifestação clínica de cara de hipopótamo diagnosticado em uma propriedade localizada no município do Capão do Leão, Rio Grande do Sul em setembro de 2016. Os bovinos permaneciam durante a maior parte do ano em restevas de arroz e quando as áreas eram ocupadas com novas lavouras eram transferidos para áreas onde havia pequenas matas nativas. Foram afetados três bovinos adultos que apresentavam aumento de volume na região nasolabial e maxilar e havia, também, tumefação dos linfonodos regionais. A evolução da enfermidade era de aproximadamente seis meses. Nos fragmentos coletados para cultura houve isolamento de Actinobacillus lignieresii. O diagnóstico foi baseado nos achados clínicos, na avaliação histopatológica caracterizada pela presença de piogranulomas com reação de Splendori Hoepli no centro, no isolamento bacteriano, identificação de Actinobacillus lignieresii por reação em cadeia da polimerase (PRC) e sequenciamento genético.(AU)

Natural lymphatic ("atypical") actinobacillosis in cattle caused by Actinobacillus lignieresii.

Bovine actinobacillosis is typically characterized by pyogranulomatous glossitis (wooden tongue). The involvement of other tissues, generally the skin or lymph nodes, has been regarded as atypical or cutaneous. We describe herein 2 outbreaks of actinobacillosis affecting primarily the lymph nodes of the head and neck. The disease affected 40 of 540 lactating cows in a dairy herd, and 5 of 335 two-y-old steers in a beef herd. Multiple or single, occasionally ulcerated nodules were observed in the region of the mandible, neck, and shoulder, including the parotid, submandibular, retropharyngeal, and prescapular lymph nodes. The histologic lesions were multifocal pyogranulomatous lymphadenitis, dermatitis, and cellulitis with Splendore-Hoeppli material. One steer had an exophytic pyogranuloma in the gingiva and another died because of ruminal tympany secondary to oropharyngeal and esophageal obstruction by a pyogranulomatous mass. Actinobacillus lignieresii was isolated from the lesions and identified by amplification, sequencing, and analysis of the 16S ribosomal (r)DNA gene. Seven of 8 cows recovered after treatment with sodium iodide. Lymphatic actinobacillosis is a frequent disease in Uruguay, southern Brazil, and Argentina. Morbidity is 1-50%; mortality is <1%. A. lignieresii apparently penetrates the intact oral and pharyngeal mucosa, infecting primarily the regional lymph nodes. Later, lesions may extend to the subcutaneous tissue and the skin, causing ulceration. Affected cattle with draining pyogranulomas contaminate the environment, favoring disease transmission, and should be treated with sodium iodide or antibiotics and isolated from the herd in order to control the disease.

Ovine genital actinobacillosis: a review.

Actinobacillus seminis infection in rams constitutes a spectrum of pathological changes in various genital organs, with a predilection for the cauda epididymis. There is a need to understand the disease, as it represents a significant factor contributing to infertility and sterility. Here, we aim to provide a comprehensive overview of the biological characteristics of A. seminis, modes of transmission, epidemiology and pathogenesis, clinical signs and pathological changes of the disease, the laboratory techniques that have been used in diagnosis, differential diagnosis, treatment and prevention, and the considerations that need to be taken into account for future research.

Actinobacillus sp. bacteremia in foals: clinical signs and prognosis.

Medical records of 101 blood culture-confirmed bacteremic foals were reviewed to determine whether foals with Actinobacillus sp. bacteremia are affected at an earlier age, have more severe signs of disease, and have a worse prognosis than do foals with bacteremia of other causes. Thirty percent (30/101) of bacteremic foals had Actinobacillus sp. cultured, and these were 2 times more likely to die (crude odds ratio [OR(CR)] 0.8, 4; P = .14), with a survival rate of 43% (13/30) compared to the overall survival rate of 55% (56/101). When compared to other bacteremic foals, foals with actinobacillosis were 7 times more likely to have been sick from birth (adjusted odds ratio [OR(ADJ)] 2, 26; P = .003) and 6 times more likely to have diarrhea (OR(ADJ) 1, 22; P = .009). By bivariate analysis. foals with Actinobacillus sp. bacteremia were 5 times more likely to have a sepsis score >11 (OR(CR) 1, 18; P = .007), 6 times more likely to be obtunded (OR(CR) 2, 20; P = .005), and 3 times more likely to have pneumonia (OR(CR) 1, 7; P = .03). Furthermore, Actinobacillus sp. bacteremic foals were 27 times more likely to have a segmented neutrophil count <3.3 X 10(9) cells/L (OR(ADJ) 4, 166: P < .0001) and were 4.5 times more likely to have a band neutrophil count >0.46 x 10(9) cells/L (OR(ADJ) 1, 17; P = .02) when compared to foals that had bacteremia caused by either gram-negative enteric or gram-positive organisms. Sepsis score was < or = 11 in 49% (29/59) of bacteremia foals aged <13 days for which a discernible sepsis score was calculable. Results of this study should improve the diagnostic sensitivity of clinical examinations of neonatal foals, thereby facilitating treatment decisions.

Characterisation of Australian isolates of Actinobacillus capsulatus, Actinobacillus equuli, Pasteurella caballi and Bisgaard Taxa 9 and 11.

OBJECTIVE: The objective of this work was to perform a comprehensive phenotypic characterisation of 16 isolates of bacteria previously identified as Actinobacillus equuli. DESIGN: The 16 isolates that had been obtained from Australian animals--15 from horses and one from a rabbit--were compared with reference strains of A equuli, A capsulatus, Pasteurella caballi and Bisgaard Taxa 9 and 11. RESULTS: The characterisation study demonstrated that only nine of the isolates were A equuli. The other isolates were identified as A capsulatus (the isolate from rabbit), P caballi (one isolate), Bisgaard Taxon 11 (two isolates) and Bisgaard Taxon 9 (one isolate). The final two isolates could not be assigned to any recognised species or taxa. CONCLUSION: This study has highlighted the importance of a complete characterisation of Actinobacillus-like organisms isolated from horses and rabbits. The study represents the first time that A capsulatus, P caballi and Bisgaard Taxa 9 and 11 have been recognised as being present in Australia.

[Serological study of the presence of Actinobacillus pleuropneumoniae antibodies serotype 2 and 9 on pig-breeding farms in the provinces of North Brabant and Overijssel (The Netherlands)]. / Serologisch onderzoek naar het voorkomen van antilichamen tegen Actinobacillus pleuropneumoniae-serotype 2 en 9 op varkensfokbedrijven in de provincies Noord-Brabant en Overijssel (Nederland).

Serological studies for the presence of CFT antibodies to Actinobacillus pleuropneumoniae, both to serotype 2 and serotype 9, were done in twenty-five sows on each of thirty pig-breeding farms in the provinces of North Brabant and Overijssel. Antibodies to serotype 2 and/or serotype 9 were detected in one or several sows on forty-two out of sixty farms. Similar findings were recorded on eighteen and twenty-four farms respectively in the provinces of Overijssel and North Brabant; the number of herds showing positive reactions to serotype 2 was sixteen and twenty-three in these provinces respectively, that showing positive reactions to serotype 9 being six and five respectively. An average proportion of nineteen per cent of the samples taken on the farms having serologically positive animals was positive for serotype 2, and twelve per cent for serotype 9. Only one of the twenty-five animals on twelve farms was found to be positive; serotype 2 was present in ten cases and serotype 9 in two cases. A marked effect of the size of the herds on the serological status of the farms was not observed in the present studies. This was also true of the purchasing policy; there was no difference between farms which had no longer purchased any breeding pigs for an average period of seventeen years and for one year respectively. Clinical symptoms of an infection due to Actinobacillus pleuropneumoniae were only reported as occurring on two farms having serologically positive animals.

Seroprevalence of Actinobacillus (Haemophilus) pleuropneumoniae serotype-1 infection in swine herds in Quebec.

Seroprevalence of Actinobacillus (Haemophilus) pleuropneumoniae serotype-1 infection was evaluated in pigs on 7 farms in Quebec. Commercial cross-bred herds A to G, ranging from 110 to 235 sows and infected with A pleuropneumoniae serotype-1 were selected. Five pigs/litter were selected at random and were identified (group 1). Blood samples were obtained from group-1 pigs at 2 to 4, 14, 28, 42, and 56 days of age. Blood also was obtained from group-1 pigs remaining in the postweaning unit at 70 days of age, and from 20 to 40 sows 1 to 3 times. To determine prevalence of seropositive pigs in all age groups for the entire study period in herds C to G, blood samples were obtained from 20 pigs/age group (group 2) selected at random at 28, 42, and 56 days of age at each visit. Group-1 pigs were included when they reached 28, 42, and 56 days of age. Pigs were serologically monitored in herds A and B for 3 months and in herds C to G for 5 to 6 months. Serologic status of pigs at 2 to 4 days of age was not statistically associated with status at 42 days (P = 0.6293) and at 56 days (P = 0.3098) of age for the same pigs. Therefore, seronegative pigs 2 to 4 days old did not seroconvert earlier than did those with detectable maternal antibodies at 2 to 4 days old. Only about 50% of the 70-day-old pigs were seropositive at 56 days. Seemingly, pigs seroconverted late in the postweaning period.(ABSTRACT TRUNCATED AT 250 WORDS)

Actinobacillosis in free-ranging snowshoe hares (Lepus americanus) from Alaska.

Actinobacillus capsulatus was isolated from lung, liver, and/or spleen tissue of three snowshoe hares (Lepus americanus) in Alaska. This is the first report of the isolation of this bacterium from free-ranging hares. Actinobacillus capsulatus may have a negative impact on the population density of hares.

Pulmonary diseases of cattle in the Kimberley District of Western Australia.

A survey of pulmonary disease in cattle slaughtered in the Kimberley district of Western Australia, incidental to the monitoring of lungs for contagious bovine pleuropneumonia (CBPP), was initiated in 1969. The results of the first 3 years are reported and indicate the complete absence of CBPP, the occurrence of actinobacillosis as the most common pulmonary lesion, the existence of phycomycosis in a few lungs each year, the frequent presence of subacute and chronic bronchopneumonias and interstitial pneumonias possibly as sequelae of actinobacillosis, tuberculosis and the pneumomycoses, and the relatively low incidence of fibrinous and necrotic pneumonias of other bacterial origin.

An unusual epizootic of actinobacillosis in dairy heifers.

Actinobacillosis developed in a group of 52 Holstein-Friesian replacement heifers. They were confined to 4 pens in a barn. Cervical abscesses were first observed by the owner in 1 heifer a month after the heifers were fed chopped haylage that was particularly dry, tough, and stemmy. During the weeks that followed, morbidity reached 73% in heifers given the feed. Calves up to 4 months old were not given the haylage and did not develop the disease. Standard bacteriologic methods were used to identify Actinobacillus lignieresii from exudate of abscesses. A biopsy of superficial granulomatous tissue of the lips revealed fibers thought to be of plant origin. Affected cattle recovered in 3 to 6 months. The lesions of 3 cattle given sodium iodide intravenously and of 4 given streptomycin regressed sooner than those given penicillin or no treatment.