RESUMEN
Radiation therapy (RT) is an effective local treatment for unresectable hepatocellular carcinoma (HCC), but there are currently no predictive biomarkers to guide treatment decision for RT or adjuvant systemic drugs to be combined with RT for HCC patients. Previously, we reported that extracts of the marine sponge Agelas sp. may contain a natural radiosensitizer for HCC treatment. In this study, we isolated (-)-agelamide D from Agelas extract and investigated the mechanism underlying its radiosensitization. (-)-Agelamide D enhanced radiation sensitivity of Hep3B cells with decreased clonogenic survival and increased apoptotic cell death. Furthermore, (-)-agelamide D increased the expression of protein kinase RNA-like endoplasmic reticulum kinase/inositol-requiring enzyme 1α/activating transcription factor 4 (PERK/eIF2α/ATF4), a key pathway of the unfolded protein response (UPR) in multiple HCC cell lines, and augmented radiation-induced UPR signaling. In vivo xenograft experiments confirmed that (-)-agelamide D enhanced tumor growth inhibition by radiation without systemic toxicity. Immunohistochemistry results showed that (-)-agelamide D further increased radiation-induced ATF4 expression and apoptotic cell death, which was consistent with our in vitro finding. Collectively, our results provide preclinical evidence that the use of UPR inducers such as (-)-agelamide D may enhance the efficacy of RT in HCC management.
Asunto(s)
Carcinoma Hepatocelular/radioterapia , Alcaloides Diterpénicos/farmacología , Neoplasias Hepáticas/radioterapia , Fármacos Sensibilizantes a Radiaciones/farmacología , Agelas/química , Animales , Línea Celular Tumoral , Alcaloides Diterpénicos/aislamiento & purificación , Humanos , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Desnudos , Fármacos Sensibilizantes a Radiaciones/aislamiento & purificación , Respuesta de Proteína Desplegada , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
Discovering anticancer drugs that do not have adverse side effects has been a developing research field worldwide in recent decades. In this work, four previously undescribed cytotoxic diterpenoids were isolated from the aerial parts of Isodon excisoides. Interestingly, these four diterpenoids were two pairs of tautomers that were first reported in plants. Their structures were further elucidated using various spectroscopic methods. The tautomerization phenomenon and mechanism for these two pairs of tautomers were emphatically described. The theoretical simulation results indicated that the diterpene tautomerization is greatly related to certain factors, including the existence of a transition state, the change of bond length and the level of conversion energy; the tautomerization for the two pairs of tautomers is mainly caused by proton transfer. For bioassays, the cytotoxicities of the tautomers against five human cancer cell lines were also investigated. The results indicated that each of the four diterpenoids showed significant cytotoxicity in at least three cell lines and could serve as potential anticancer agents for further investigation.
Asunto(s)
Antineoplásicos/química , Neoplasias del Colon/tratamiento farmacológico , Alcaloides Diterpénicos/química , Antineoplásicos/aislamiento & purificación , Antineoplásicos/farmacología , Apoptosis , Alcaloides Diterpénicos/aislamiento & purificación , Alcaloides Diterpénicos/farmacología , Descubrimiento de Drogas , Células HCT116 , Humanos , Isodon , Estructura Molecular , Análisis EspectralRESUMEN
A new C19-diterpenoid alkaloid named hemsleyaline (1), along with fourteen known alkaloids (2-15), were isolated from the roots of Aconitum hemsleyanum Pritz. (Ranunculaceae), a herbal medicine in southwest China. Their structures were established on the basis of extensive spectroscopic analyses. Compound 1 showed mild cholinesterase inhibitory effect with IC50 value of 471 ± 9 µM.
Asunto(s)
Aconitum/química , Inhibidores de la Colinesterasa/farmacología , Alcaloides Diterpénicos/química , Alcaloides Diterpénicos/farmacología , Inhibidores de la Colinesterasa/química , Alcaloides Diterpénicos/aislamiento & purificación , Medicamentos Herbarios Chinos/química , Espectroscopía de Resonancia Magnética , Estructura Molecular , Raíces de Plantas/químicaRESUMEN
Ten new prenylated indole diterpene alkaloids, tolypocladin A-J (1-10), including four chlorinated metabolites, have been isolated from a culture of a mine-soil-derived fungus, Tolypocladium sp. XL115. The structures and absolute configurations of 1-10 were determined by spectroscopic analysis, ECD calculations, and comparison with known compounds. Compounds 1 and 8 displayed significant antimicrobial activities. In addition, compound 1 also showed weak cytotoxic activity against all tested human cancer cell lines and suppressed the growth and viability of the patient-derived HCC cells T1224.
Asunto(s)
Antiinfecciosos/aislamiento & purificación , Alcaloides Diterpénicos/aislamiento & purificación , Hypocreales/metabolismo , Indoles/aislamiento & purificación , Microbiología del Suelo , Línea Celular Tumoral , Alcaloides Diterpénicos/química , Alcaloides Diterpénicos/farmacología , Humanos , Espectroscopía de Resonancia MagnéticaRESUMEN
One new indole diterpenoid, drechmerin I (1), was isolated from the fermentation broth of Drechmeria sp. isolated from the root of Panax notoginseng. Its structure was elucidated based on 1 D and 2 D nuclear magnetic resonance (NMR), high resolution electrospray ionization mass spectrum (HRESIMS), and electronic circular dichroism (ECD) spectroscopic analyses as well as TD DFT calculations of ECD spectra. Drechmerin I (1) was assayed for its antimicrobial activity against Candida albicans, Staphylococcus aureus, Bacillus cereus, B. subtillis, Pseudomonas aeruginosa, and Klebsiella pneumonia, respectively. Drechmerin I (1) showed antimicrobial activities against B. subtillis with an MIC value of 200 µg/mL. The interaction of S. aureus peptide deformylase with drechmerin I (1) was investigated by molecular docking.