RESUMEN
Food processing methods may influence the health of dogs. However, previous studies have mostly been based on a comparison of several commercial dog foods with different ingredients. In this study, eighteen adult beagles of the same age and health status (assessed by routine blood tests) were used in the experiments. This study analyzed the effects of the following different processing methods: raw, pasteurized, and high temperature sterilization (HTS) made with the same ingredients and nutrients (based on dry matter) on serum parameters, apparent total-tract macronutrient digestibility, fecal microbiota and short-chain fatty acid (SCFA) content in beagle dogs. The data showed, after a test lasting 56-days, the apparent digestibility (ATTD) of protein and fat in HTS food was 91.9%, which was significantly higher (P< 0.05) than that in dry food (89.2%, P < 0.05). The serum content of triglyceride increased in beagles fed HTS food (P < 0.05), and the number of neutrophils in beagles fed raw food and pasteurized food increased significantly (P < 0.05), and the platelet count in beagles fed raw food showed an increasing trend compared with the beagles fed HTS food. Different processing methods had an impact on the intestinal microbiota and SCFA of beagles; at least 14 genera were significantly affected by the food produced using different processing methods. In particular, the abundance of Allprevotella, Escherichia-Shigella and Turicibacter, and the total acid content were lower in beagles fed the raw diet, whereas Streptococcus, Collinsella, Bacteroides and Ruminococcus gnavus were more abundant following the HTS diet, and Lactococcus showed the highest abundance in beagles fed the pasteurized diet. This study showed that dog food produced by different processing methods affected the health of adult beagles.
Asunto(s)
Alimentación Animal/análisis , Dieta/métodos , Manipulación de Alimentos/métodos , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Dieta/veterinaria , Digestión , Perros , Heces/química , Femenino , Microbioma Gastrointestinal/genética , Tracto Gastrointestinal/metabolismo , Masculino , Microbiota/genética , Nutrientes , Pasteurización/métodos , Alimentos Crudos/efectos adversosRESUMEN
Trichinellosis is a parasitic infection that is associated with the consumption of raw meat. The specific genotype Trichinella nativa has been found in raw bear meat. The most common genotype that has been linked with myocarditis is T spiralis. We present a case of T nativa myocarditis secondary to consumption of raw bear meat. The clinical manifestations as well as therapy of this specific genotype is outlined.
Asunto(s)
Carne/parasitología , Mebendazol/administración & dosificación , Miocarditis , Prednisona/administración & dosificación , Músculo Cuádriceps/patología , Trichinella , Triquinelosis , Adulto , Animales , Antiinflamatorios/administración & dosificación , Antinematodos/administración & dosificación , Biopsia/métodos , Femenino , Pruebas de Función Cardíaca/métodos , Humanos , Imagen por Resonancia Cinemagnética/métodos , Miocarditis/sangre , Miocarditis/tratamiento farmacológico , Miocarditis/etiología , Miocarditis/fisiopatología , Alimentos Crudos/efectos adversos , Alimentos Crudos/parasitología , Pruebas Serológicas/métodos , Resultado del Tratamiento , Trichinella/genética , Trichinella/aislamiento & purificación , Triquinelosis/diagnóstico , Triquinelosis/tratamiento farmacológico , Triquinelosis/etiología , Triquinelosis/fisiopatologíaAsunto(s)
Eritema/etiología , Exantema/etiología , Hipersensibilidad a los Alimentos/complicaciones , Hongos Shiitake , Hipersensibilidad a los Alimentos/diagnóstico , Hipersensibilidad a los Alimentos/patología , Humanos , Masculino , Persona de Mediana Edad , Alimentos Crudos/efectos adversos , Pruebas CutáneasRESUMEN
Clonorchiasis is caused by raw-freshwater fish-eating practice and causes high burden in Asia. Transmission mechanism of this behavior hasn't been illuminated, which hinders the adoption of sustainable control activities. A cross-sectional survey was implemented in students from four endemic provinces in China. Data with 23,222 students aged 9-18 and their parents were eligible. Familial clustering of raw-eating practice, impact of parents' practice on children, interaction of spouses' practice was analyzed. Raw-eating practice met ß-binomial distribution (χ2 = 0.8, p>0.05). Clustering coefficient increased by students' age (R2 = 0.82, p<0.001) and was higher in those families with boys compared to girls (t = 4.1, p<0.01). The proportion of students with raw-eating practice increased yearly by 8.9% in girls and 10.5% in boys. Compared to those without parents' raw-eating practice, adjusted odds ratio of students' raw-eating practice was 10.5 (95% confidential intervals (95% CI): 9.4-11.7) in those with fathers' practice, 33.6 (95% CI: 26.3-42.9) in those with mothers' practice and 47.1 (95% CI: 42.0-52.8) in those with both parents' practice. There existed interaction between spouses' practice (χ2 = 6713.1, p<0.001) and the impact from husband on his wife was higher than that from wife on her husband. Familial assimilation characterizes the transmission of raw-freshwater fish-eating practice, consisted of vertical intergenerational assimilation from parents to their children and horizontal martial assimilation between spouses. A sustainable strategy against clonorchiasis should interrupt the transmission of raw-freshwater fish-eating practice. Additionally, further studies are expected to explore more information, e.g. the frequency in raw-eating practice and type of raw freshwater fish, infection status of C. sinensis in participants, as well as direct collection of parents' eating information from themselves.
Asunto(s)
Clonorquiasis/epidemiología , Salud de la Familia , Conducta Alimentaria , Enfermedades Transmitidas por los Alimentos/epidemiología , Alimentos Crudos/efectos adversos , Adolescente , Terapia Conductista , Niño , China/epidemiología , Clonorquiasis/prevención & control , Femenino , Enfermedades Transmitidas por los Alimentos/prevención & control , Humanos , Masculino , Encuestas y CuestionariosAsunto(s)
Alimentación Animal , Alimentos Crudos/efectos adversos , Animales , Humanos , Mascotas , RiesgoAsunto(s)
Anisakiasis , Anisakis/aislamiento & purificación , Colonoscopía/métodos , Productos Pesqueros/efectos adversos , Íleon , Alimentos Crudos/efectos adversos , Anciano , Animales , Anisakiasis/diagnóstico , Anisakiasis/etiología , Anisakiasis/fisiopatología , Anisakiasis/terapia , Humanos , Íleon/diagnóstico por imagen , Íleon/parasitología , Hallazgos Incidentales , Masculino , Resultado del Tratamiento , Zoonosis/diagnóstico , Zoonosis/etiología , Zoonosis/fisiopatología , Zoonosis/prevención & controlRESUMEN
Anisakiasis is a zoonotic parasitic disease caused by consumption of raw or undercooked fish or seafood infected with nematodes of the Anisakis, Pseudoterranova or Contracaecum genera. Here, we describe the first case of anisakiasis in Colombia and summarize the available literature. A 52-year-old female with a history of abrupt-onset sharp epigastric pain, nausea, vomit, diarrhea, and urticaria following fish consumption consulted the health service. The physical examination revealed moderate tenderness of the epigastric region; the laboratory evaluation showed leukocytosis and a simple X-ray and ECG showed no abnormalities. The diagnosis was made by endoscopic examination, which revealed a thickened gastric wall and a moving larval worm. An Anisakis larva was found and extracted endoscopically, which relieved the pain of the patient. Clinically, anisakiasis may present as a gastric, intestinal, extragastrointestinal or allergic disease. Diagnosis and treatment of anisakiasis are made by a dietary history, direct visualization and endoscopic extraction of possible larvae, which is the only effective therapy.
La anisakiasis es una enfermedad parasitaria zoonótica causada por el consumo de pescados o mariscos crudos o poco cocidos infectados con nematodos de los géneros Anisakis, Pseudoterranova y Contracaecum. Se describe el primer caso de anisakiasis en Colombia y se resume la literatura médica disponible. Una mujer de 52 años de edad consultó por dolor epigástrico agudo de inicio abrupto, náuseas, vómitos, diarrea y urticaria después de consumir pescado. El examen físico reveló sensibilidad moderada en el epigastrio. El examen de laboratorio evidenció leucocitosis, en tanto que la radiografía simple y el electrocardiograma no reflejaron ninguna anormalidad. El diagnóstico se hizo mediante una endoscopia de vías digestivas altas, la cual reveló engrosamiento de la pared gástrica y un parásito en movimiento. Se encontró una larva de Anisakis y se la extrajo por endoscopia, lo que alivió el dolor de la paciente. Clínicamente, la anisakiasis puede presentarse como una enfermedad gástrica, intestinal, en otros sistemas o alérgica. El diagnóstico se hace con base en la elaboración del historial alimentario del paciente y la visualización directa de las larvas; el único tratamiento efectivo consiste en su extracción endoscópica.
Asunto(s)
Anisakiasis/diagnóstico , Anisakis/aislamiento & purificación , Peces/parasitología , Parasitología de Alimentos , Alimentos Crudos/efectos adversos , Gastropatías/parasitología , Urticaria/etiología , Albendazol/uso terapéutico , Animales , Anisakiasis/tratamiento farmacológico , Anisakiasis/inmunología , Anisakiasis/cirugía , Anisakis/crecimiento & desarrollo , Antihelmínticos/uso terapéutico , Colombia , Terapia Combinada , Femenino , Gastroscopía , Humanos , Larva , Persona de Mediana Edad , Alimentos Crudos/parasitología , Gastropatías/diagnóstico , Gastropatías/inmunologíaRESUMEN
Acalculous cholecystitis etiologies while numerous, some of them are less-known such as brucellosis. In this report, we elaborate the clinical findings, investigations and management of two female patients presenting acalculous cholecystitis in whom diagnosis of acute brucellosis was retained. Both patients had fever, asthenia and abdominal tenderness. Laboratory results showed evidence of inflammation as well as hepatic cytolysis while cholestasis was noted in one patient. In both cases, ultrasound study and CT confirmed the presence of acalculous cholecystitis. Serology (tube agglutination test) led to the diagnosis of brucellosis. Diagnosis of brucellosis-related acute cholecystitis was established in both cases based on imaging findings as well as serology without resorting to cholecystectomy. Favourable clinical response to specific antibiotic therapy further supported our diagnosis as well as our decision to avoid surgery. Although few cases have been reported, brucellosis must be considered as a cause of acalculous cholecystitis, especially in endemic countries.
Asunto(s)
Colecistitis Alitiásica/microbiología , Antibacterianos/uso terapéutico , Brucelosis/complicaciones , Doxiciclina/uso terapéutico , Leche/microbiología , Alimentos Crudos/efectos adversos , Rifampin/uso terapéutico , Dolor Abdominal/microbiología , Colecistitis Alitiásica/diagnóstico por imagen , Colecistitis Alitiásica/tratamiento farmacológico , Enfermedad Aguda , Animales , Brucelosis/diagnóstico por imagen , Brucelosis/tratamiento farmacológico , Brucelosis/fisiopatología , Proteína C-Reactiva/metabolismo , Femenino , Contaminación de Alimentos , Humanos , Persona de Mediana Edad , Resultado del Tratamiento , Ultrasonografía , Vómitos , Adulto JovenRESUMEN
Although the major risk factors for liver cancer have been established, preventive factors for liver cancer have not been fully explored. We evaluated the association between raw garlic consumption and liver cancer in a large population-based case-control study in Eastern China. The study was conducted in Jiangsu, China, from 2003 to 2010. A total of 2011 incident liver cancer cases and 7933 randomly selected population-controls were interviewed. Epidemiological data including raw garlic intake and other exposures were collected, and serum markers of hepatitis B virus (HBV) and hepatitis C virus (HCV) infection were assayed. Overall, eating raw garlic twice or more per week was inversely associated with liver cancer, with an adjusted odds ratio (aOR) of 0.77 (95% confidence interval (CI): 0.62-0.96) compared to those ingesting no raw garlic or less than twice per week. In stratified analyses, high intake of raw garlic was inversely associated with liver cancer among Hepatitis B surface antigen (HBsAg) negative individuals, frequent alcohol drinkers, those having history of eating mold-contaminated food or drinking raw water, and those without family history of liver cancer. Marginal interactions on an additive scale were observed between low raw garlic intake and HBsAg positivity (attributable proportion due to interaction (AP) = 0.31, 95% CI: -0.01-0.62) and heavy alcohol drinking (AP = 0.28, 95% CI: 0.00-0.57). Raw garlic consumption is inversely associated with liver cancer. Such an association shed some light on the potential etiologic role of garlic intake on liver cancer, which in turn might provide a possible dietary intervention to reduce liver cancer in Chinese population.
Asunto(s)
Dieta/efectos adversos , Ajo/efectos adversos , Neoplasias Hepáticas/epidemiología , Neoplasias Hepáticas/etiología , Alimentos Crudos/efectos adversos , Adulto , Anciano , Estudios de Casos y Controles , China/epidemiología , Dieta/métodos , Femenino , Humanos , Masculino , Persona de Mediana Edad , Oportunidad Relativa , Factores de RiesgoRESUMEN
There has been concerns related to the risk of bacterial contamination from raw pet food to humans, but research is still scarce. The purpose of this cross-sectional study was to use a worldwide internet survey-based data to evaluate the impact of raw pet foods on human health from the owners' experience. From 16 475 households, 0.2 per cent (n=39) reported having had a transmission of pathogen from the raw pet food to a human family member during the time that raw feeding had been used in the household. Only in three of those households the same pathogen that was found in the human sample was analysed and confirmed also in the raw pet food (0.02 per cent of all data). Moreover, 0.1 per cent (n=24) reported suspecting that a disease could have been transmitted to a human from the pet food. Feeding salmon and turkey, using more than 50 per cent of the diet as raw foods and preparing the raw food in the same place and utensils as the family foods all had negative association with infections. Having 2 to 6 year-old children living in the household was associated with more infections, although adults were the most frequently infected.
Asunto(s)
Alimentación Animal/efectos adversos , Infecciones/etiología , Alimentos Crudos/efectos adversos , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Animales , Estudios Transversales , Femenino , Humanos , Internet , Masculino , Persona de Mediana Edad , Propiedad , Percepción , Encuestas y Cuestionarios , Adulto JovenRESUMEN
Cutaneous gnathostomiasis (CG) is a disease caused by ingestion of third-stage Gnathostoma larva in raw snakes, freshwater fish or frogs. The common causative organisms of CG in Japan include G. nipponicum, G. spinigerum, G. doloresi, G. binucleatum and G. hispidum. We report two cases of CG after eating many raw Japanese icefishes (Salangichthys microdon). In both cases, linear itchy eruptions on the trunk developed after eating many S. microdon. We performed genetic analysis in the first case, which revealed G. nipponicum. Of note, this is the first case of CG diagnosed based on genetic analysis in Japan. In Japan, eating whole small raw freshwater fish is common. The most popular types of raw small freshwater fish consumed in Japan are S. microdon (shirauo in Japanese) and Leucopsarion petersii (shirouo in Japanese). Usually, S. microdon are born in rivers, but live in both the sea and rivers. They feed on small fish and freshwater water fleas and spawn in rivers in the spring. On the other hand, L. petersii are born in rivers, but move to the sea soon after hatching. They feed on plankton such as copepod in the sea. They do not feed on anything when they return to rivers to spawn in the spring. Therefore, we hypothesize that S. microdon are more easily parasitized by G. nipponicum.
Asunto(s)
Peces/parasitología , Gnathostomiasis/diagnóstico , Alimentos Crudos/efectos adversos , Enfermedades Cutáneas Parasitarias/diagnóstico , Zoonosis/diagnóstico , Anciano , Animales , Femenino , Gnathostoma/aislamiento & purificación , Gnathostomiasis/parasitología , Gnathostomiasis/transmisión , Humanos , Japón , Masculino , Piel/parasitología , Piel/patología , Enfermedades Cutáneas Parasitarias/parasitología , Enfermedades Cutáneas Parasitarias/transmisión , Adulto Joven , Zoonosis/parasitología , Zoonosis/transmisiónRESUMEN
Resumen La anisakiasis es una enfermedad parasitaria zoonótica causada por el consumo de pescados o mariscos crudos o poco cocidos infectados con nematodos de los géneros Anisakis, Pseudoterranova y Contracaecum. Se describe el primer caso de anisakiasis en Colombia y se resume la literatura médica disponible. Una mujer de 52 años de edad consultó por dolor epigástrico agudo de inicio abrupto, náuseas, vómitos, diarrea y urticaria después de consumir pescado. El examen físico reveló sensibilidad moderada en el epigastrio. El examen de laboratorio evidenció leucocitosis, en tanto que la radiografía simple y el electrocardiograma no reflejaron ninguna anormalidad. El diagnóstico se hizo mediante una endoscopia de vías digestivas altas, la cual reveló engrosamiento de la pared gástrica y un parásito en movimiento. Se encontró una larva de Anisakis y se la extrajo por endoscopia, lo que alivió el dolor de la paciente. Clínicamente, la anisakiasis puede presentarse como una enfermedad gástrica, intestinal, en otros sistemas o alérgica. El diagnóstico se hace con base en la elaboración del historial alimentario del paciente y la visualización directa de las larvas; el único tratamiento efectivo consiste en su extracción endoscópica.
Abstract Anisakiasis is a zoonotic parasitic disease caused by consumption of raw or undercooked fish or seafood infected with nematodes of the Anisakis, Pseudoterranova or Contracaecum genera. Here, we describe the first case of anisakiasis in Colombia and summarize the available literature. A 52-year-old female with a history of abrupt-onset sharp epigastric pain, nausea, vomit, diarrhea, and urticaria following fish consumption consulted the health service. The physical examination revealed moderate tenderness of the epigastric region; the laboratory evaluation showed leukocytosis and a simple X-ray and ECG showed no abnormalities. The diagnosis was made by endoscopic examination, which revealed a thickened gastric wall and a moving larval worm. An Anisakis larva was found and extracted endoscopically, which relieved the pain of the patient. Clinically, anisakiasis may present as a gastric, intestinal, extragastrointestinal or allergic disease. Diagnosis and treatment of anisakiasis are made by a dietary history, direct visualization and endoscopic extraction of possible larvae, which is the only effective therapy.
Asunto(s)
Animales , Femenino , Humanos , Persona de Mediana Edad , Gastropatías/parasitología , Urticaria/etiología , Parasitología de Alimentos , Anisakis/aislamiento & purificación , Anisakiasis/diagnóstico , Peces/parasitología , Alimentos Crudos/efectos adversos , Gastropatías/diagnóstico , Gastropatías/inmunología , Albendazol/uso terapéutico , Gastroscopía , Anisakis/crecimiento & desarrollo , Anisakiasis/cirugía , Anisakiasis/inmunología , Anisakiasis/tratamiento farmacológico , Colombia , Terapia Combinada , Alimentos Crudos/parasitología , Larva , Antihelmínticos/uso terapéuticoRESUMEN
OBJECTIVES: Mycobacterium bovis, a member of the Mycobacterium tuberculosis complex, can infect cats and has proven zoonotic risks for owners. Infected cats typically present with a history of outdoor lifestyle and hunting behaviour, and cutaneous granulomas are most commonly observed. The aim of this study is to describe an outbreak of tuberculous disease commencing with six young cats, living exclusively indoors in five different households across England, being presented to separate veterinarians across the UK with a variety of clinical signs. METHODS: Investigations into the pyogranulomatous lesions, lymphadenopathy and/or pulmonary disease of these cases consistently identified infection with M bovis. Infection was confirmed by PCR, where possible, or was indicated with a positive interferon-gamma release assay (IGRA), where material for PCR was unavailable. In-contact, cohabiting cats were screened by IGRA and follow-up testing was undertaken/advised where results were positive. A lifestyle investigation was undertaken to identify the source of infection. RESULTS: Six clinically sick cats and seven in-contact cats were identified with evidence of M bovis infection. Five clinical cases were either too sick to treat or deteriorated despite therapy, giving a mortality rate of 83%. Lifestyle investigations revealed the common factors between clusters to be that affected cats had mycobacterial infections speciated to M bovis, were exclusively indoor cats and were fed a commercially available raw food product produced by a single manufacturer. The Food Standards Agency, Animal & Plant Health Agency, Public Health England and the food manufacturer concerned have been notified/informed. Other possible sources of exposure for these cats to M bovis were explored and were excluded, including wildlife contact, access to raw milk, the presence of rodent populations inside the buildings in which the cats lived and exposure to known infectious humans. CONCLUSIONS AND RELEVANCE: Upon investigations, our results provide compelling, if circumstantial, evidence of an association between the commercial raw diet of these cats and their M bovis infections.
Asunto(s)
Enfermedades de los Gatos , Brotes de Enfermedades/veterinaria , Mycobacterium bovis , Alimentos Crudos/efectos adversos , Tuberculosis , Alimentación Animal/efectos adversos , Animales , Enfermedades de los Gatos/etiología , Enfermedades de los Gatos/microbiología , Gatos , Inglaterra , Tuberculosis/etiología , Tuberculosis/microbiología , Tuberculosis/veterinariaRESUMEN
Neurolisteriosis is known to affect vulnerable groups, for example neonates or children with immunodeficiency. This is a key point of the current clinical guidelines regarding pediatric meningitis. We report a rare case of neurolisteriosis in an immunocompetent infant, without the typical signs of listeriosis, which led to a delay in administering the appropriate antibiotherapy. This case illustrates the clinical heterogeneity of neurolisteriosis and the relevance of appropriate polymerase chain reaction (PCR) tests when the clinical presentation differs from the current guidelines. This case also reminds us that raw or unpasteurized milk-based food products pose a risk even in immunocompetent infants or children.
Asunto(s)
Inmunocompetencia , Meningitis por Listeria/diagnóstico , ADN Bacteriano , Fiebre/microbiología , Humanos , Recién Nacido , Listeria monocytogenes/genética , Reacción en Cadena de la Polimerasa , Alimentos Crudos/efectos adversos , Punción EspinalRESUMEN
Streptococcus suis is a Gram-positive cocci bacterium that are found mainly in pigs and can be transmitted to human through pigs or pork exposure. The disease is mainly found among occupations involving swine contact in western countries whereas in Asia the disease is usually contracted through raw pork consumption. In this case report, we present a case of a middle-aged Thai man who acquired the infection from raw pork consumption. He presented with endogenous endophthalmitis with infective spondylodiscitis, sepsis and meningitis and later developed blindness of the right eye and permanent bilateral hearing loss disseminated from S. suis infection. Our report suggests that S. suis infection be considered as a causative factor in patient presenting with established clinical symptoms and predisposing factors. Cultural habit of eating raw pork should be taken into account especially in Asian countries.
Asunto(s)
Sordera/diagnóstico , Sordera/microbiología , Endoftalmitis/diagnóstico , Alimentos Crudos/efectos adversos , Carne Roja/efectos adversos , Infecciones Estreptocócicas/diagnóstico , Streptococcus suis/aislamiento & purificación , Sordera/complicaciones , Endoftalmitis/complicaciones , Endoftalmitis/microbiología , Humanos , Masculino , Persona de Mediana Edad , Infecciones Estreptocócicas/complicacionesAsunto(s)
Enfermedades de los Gatos/microbiología , Mycobacterium bovis/aislamiento & purificación , Mascotas/microbiología , Tuberculosis/veterinaria , Animales , Enfermedades de los Gatos/diagnóstico , Gatos , Diagnóstico Diferencial , Inglaterra , Linaje , Alimentos Crudos/efectos adversos , Tuberculosis/diagnóstico , Tuberculosis/microbiologíaRESUMEN
No disponible
Asunto(s)
Humanos , Masculino , Femenino , Adulto , Persona de Mediana Edad , Diarrea/etiología , Enfermedades Transmitidas por los Alimentos/diagnóstico , Productos Pesqueros/efectos adversos , Alimentos Crudos/efectos adversosRESUMEN
Foodborne non-typhoidal salmonellosis causes approximately 1 million illnesses annually in the USA. In April 2015, we investigated a multistate outbreak of 65 Salmonella Paratyphi B variant L(+) tartrate(+) infections associated with frozen raw tuna imported from Indonesia, which was consumed raw in sushi. Forty-six (92%) of 50 case-patients interviewed ate sushi during the week before illness onset, and 44 (98%) of 45 who specified ate sushi containing raw tuna. Two outbreak strains were isolated from the samples of frozen raw tuna. Traceback identified a single importer as a common source of tuna consumed by case-patients; this importer issued three voluntary recalls of tuna sourced from one Indonesian processor. Four Salmonella Weltevreden infections were also linked to this outbreak. Whole-genome sequencing was useful in establishing a link between Salmonella isolated from ill people and tuna. This outbreak highlights the continuing foodborne illness risk associated with raw seafood consumption, the importance of processing seafood in a manner that minimises contamination with pathogenic microorganisms and the continuing need to ensure imported foods are safe to eat. People at higher risk for foodborne illness should not consume undercooked animal products, such as raw seafood.