RESUMEN
Enzymatic assays based on Fructosyl Amino Acid Oxidases (FAOX) represent a potential, rapid and economical strategy to measure glycated hemoglobin (HbA1c), which is in turn a reliable method to monitor the insurgence and the development of diabetes mellitus. However, the engineering of naturally occurring FAOX to specifically recognize fructosyl-valine (the glycated N-terminal residue of HbA1c) has been hindered by the paucity of information on the tridimensional structures and catalytic residues of the different FAOX that exist in nature, and in general on the molecular mechanisms that regulate specificity in this class of enzymes. In this study, we use molecular dynamics simulations and advanced modeling techniques to investigate five different relevant wild-type FAOX (Amadoriase I, Amadoriase II, PnFPOX, FPOX-E and N1-1-FAOD) in order to elucidate the molecular mechanisms that drive their specificity towards polar and nonpolar substrates. Specifically, we compare these five different FAOX in terms of overall folding, ligand entry tunnels, ligand binding residues and ligand binding energies. Our work will contribute to future enzyme structure modifications aimed at the rational design of novel biosensors for the monitoring of blood glucose levels.
Asunto(s)
Aminoácido Oxidorreductasas/química , Simulación de Dinámica Molecular , Aminoácido Oxidorreductasas/clasificación , Aminoácido Oxidorreductasas/genética , Aminoácido Oxidorreductasas/metabolismo , Secuencia de Aminoácidos , Secuencia Conservada , Enlace de Hidrógeno , Interacciones Hidrofóbicas e Hidrofílicas , Ligandos , Conformación Molecular , Familia de Multigenes , Filogenia , Sales (Química)/química , Relación Estructura-Actividad , Especificidad por SustratoRESUMEN
BACKGROUND: L-Amino acid oxidases (LAOs) have been generally described as flavoproteins that oxidize amino acids releasing the corresponding ketoacid, ammonium and hydrogen peroxide. The generation of hydrogen peroxide gives to these enzymes antimicrobial characteristics. They are involved in processes such as biofilm development and microbial competition. LAOs are of great biotechnological interest in different applications such as the design of biosensors, biotransformations and biomedicine. The marine bacterium Marinomonas mediterranea synthesizes LodA, the first known LAO that contains a quinone cofactor. LodA is encoded in an operon that contains a second gene coding for LodB, a protein required for the post-translational modification generating the cofactor. Recently, GoxA, a quinoprotein with sequence similarity to LodA but with a different enzymatic activity (glycine oxidase instead of lysine-ε-oxidase) has been described. The aim of this work has been to study the distribution of genes similar to lodA and/or goxA in sequenced microbial genomes and to get insight into the evolution of this novel family of proteins through phylogenetic analysis. RESULTS: Genes encoding LodA-like proteins have been detected in several bacterial classes. However, they are absent in Archaea and detected only in a small group of fungi of the class Agaromycetes. The vast majority of the genes detected are in a genome region with a nearby lodB-like gene suggesting a specific interaction between both partner proteins. Sequence alignment of the LodA-like proteins allowed the detection of several conserved residues. All of them showed a Cys and a Trp that aligned with the residues that are forming part of the cysteine tryptophilquinone (CTQ) cofactor in LodA. Phylogenetic analysis revealed that LodA-like proteins can be clustered in different groups. Interestingly, LodA and GoxA are in different groups, indicating that those groups are related to the enzymatic activity of the proteins detected. CONCLUSIONS: Genome mining has revealed for the first time the broad distribution of LodA-like proteins containing a CTQ cofactor in many different microbial groups. This study provides a platform to explore the potentially novel enzymatic activities of the proteins detected, the mechanisms of post-translational modifications involved in their synthesis, as well as their biological relevance.
Asunto(s)
Aminoácido Oxidorreductasas/genética , Proteínas Bacterianas/genética , Genoma Bacteriano , Marinomonas/genética , Aminoácido Oxidorreductasas/clasificación , Secuencia de Aminoácidos , Proteínas Bacterianas/química , Proteínas Bacterianas/clasificación , Biología Computacional , Datos de Secuencia Molecular , Filogenia , Alineación de SecuenciaRESUMEN
KEY MESSAGE: TaACO1 could catalyze ACC into ethylene in vitro. Constitutive expression of TaACO1 in Arabidopsis conferred salt sensitivity, and TaACO1 regulates salt stress mainly via the DREB1/CBF signal transduction pathway. Ethylene signaling plays essential roles in mediating plant responses to biotic and abiotic stresses, besides regulating plant growth and development. The roles of ethylene biosynthesis in abiotic stress, however, remain elusive. In this study, an aminocyclopropane-1-carboxylate oxidase gene, TaACO1, affecting the terminal step in ethylene biosynthesis, was isolated from a salt-tolerant bread wheat introgression line Shanrong No. 3 (SR3) and its effect on salt-stress response was examined. Purified recombinant protein of TaACO1 heterogenously expressed in Escherchia coli could catalyze ACC into ethylene in vitro. TaACO1 transcripts were down-regulated by salt, drought, oxidative stress and ABA. TaACO1-transgenic plants conferred salt sensitivity as judged from the seed germination, cotyledon greening and the relative root growth under salt stress. Constitutive expression of TaACO1 in Arabidopsis increased AtMYB15 expression and suppressed the expression of stress-responsive genes AtRAB18, AtCBF1 and AtCBF3. These findings are helpful in understanding the roles of ethylene biosynthesis in plant salt-stress response.
Asunto(s)
Aminoácido Oxidorreductasas/genética , Arabidopsis/genética , Proteínas de Plantas/genética , Tolerancia a la Sal/genética , Triticum/genética , Ácido Abscísico/farmacología , Aminoácido Oxidorreductasas/clasificación , Aminoácido Oxidorreductasas/metabolismo , Aminoácidos Cíclicos/metabolismo , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Núcleo Celular/enzimología , Citoplasma/enzimología , Sequías , Etilenos/metabolismo , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Germinación/genética , Microscopía Confocal , Filogenia , Reguladores del Crecimiento de las Plantas/farmacología , Proteínas de Plantas/metabolismo , Raíces de Plantas/enzimología , Raíces de Plantas/genética , Raíces de Plantas/metabolismo , Plantas Modificadas Genéticamente , Proteínas Recombinantes/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Salinidad , Semillas/enzimología , Semillas/genética , Semillas/metabolismo , Cloruro de Sodio/farmacología , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Triticum/enzimología , Triticum/metabolismoRESUMEN
A novel tryptophan assay was developed using tryptophan oxidases. Although many l-amino acid oxidases (LAAOs) have been reported to catalyze tryptophan oxidation, most of them have broad substrate specificity and oxidize multiple amino acids besides tryptophan. To obtain a tryptophan-specific LAAO, we focused on bis-indole antibiotic biosynthesis, a bacterial secondary metabolic pathway. A putative LAAO from Streptomyces sp. TP-A0274, StaO involved in staurosporine biosynthesis, was heterologously expressed, biochemically characterized, and shown to serve as a selective tryptophan oxidase for the first time. In addition, another LAAO, VioA involved in violacein biosynthesis in Chromobacterium violaceum, was characterized for comparison with StaO. Interestingly, StaO and VioA share similar properties, namely narrow substrate specificity and high affinity for l-tryptophan, despite the phylogenetic distance between these enzymes. Owing to these features, uncommon among known LAAOs, StaO and VioA assays can be used for selective and accurate quantification of l-tryptophan via a coupled colorimetric reaction. Indeed, StaO and VioA assays provided tryptophan concentrations in human plasma as accurately as those obtained by high-performance liquid chromatography. Therefore, these enzymes were clearly shown to offer an effective method for determining tryptophan in biological samples rapidly, inexpensively, and accurately. The results shown here also suggest the possibility of metabolism-oriented screening as a strategy to obtain enzymes highly selective for individual biomolecules.
Asunto(s)
Antibacterianos/biosíntesis , Oxidorreductasas/metabolismo , Triptófano/química , Aminoácido Oxidorreductasas/clasificación , Aminoácido Oxidorreductasas/genética , Aminoácido Oxidorreductasas/metabolismo , Secuencia de Aminoácidos , Bacterias/enzimología , Clonación Molecular , Humanos , Estructura Molecular , Filogenia , Especificidad de la Especie , Especificidad por Sustrato , Triptófano/sangreRESUMEN
A cDNA encoding the putative octopine dehydrogenase (OcDH) from the mussel Mytilus galloprovincialis was cloned and sequenced. The complete coding region was expressed in the bacteria Escherichia coli and the recombinant protein was purified. The M. galloprovincialis OcDH appears to have the highest affinity for the amino acid substrate L-arginine (88.22%), compared to L-alanine (9.04%) and glycine (2.74%). This enzyme showed no activity when taurine or ß-alanine was used as substrate. These data strongly support that this recombinant enzyme is octopine dehydrogenase and not another opine dehydrogenase such as alanopine or strombine dehydrogenases. The superimposition of the theoretical three-dimensional model of the M. galloprovincialis OcDH and the crystal structure of its homologous counterpart from the great scallop Pecten maximus showed interesting changes in the amino acid binding site which could explain the differences found in the substrate affinity between the two molluscs. A phylogenetic analysis was performed comparing M. galloprovincialis OcDH and annotated sequences representing the five opine dehydrogenase (OpDH) protein family members. The phylogenetic tree which was obtained clustered the OpDH enzymes according to the evolutionary relationships of the species and not to the biochemical reaction catalysed. Octopine dehydrogenase has been identified in the Mytilidae family for the first time, having previously only been established in one other marine invertebrate (P. maximus).
Asunto(s)
Aminoácido Oxidorreductasas/química , Aminoácido Oxidorreductasas/metabolismo , Mytilus/enzimología , Alanina/metabolismo , Aminoácido Oxidorreductasas/clasificación , Aminoácido Oxidorreductasas/genética , Secuencia de Aminoácidos , Animales , Arginina/metabolismo , Secuencia de Bases , Glicina/metabolismo , Modelos Moleculares , Datos de Secuencia Molecular , Filogenia , Estructura Terciaria de Proteína , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Alineación de Secuencia , Especificidad por SustratoRESUMEN
S'adenosyl-L: -methionine (SAM) is a ubiquitous methyl donor and a precursor in the biosynthesis of ethylene, polyamines, biotin, and nicotianamine in plants. Only limited information is available regarding its synthesis (SAM cycle) and its concentrations in plant tissues. The SAM concentrations in flowers of Nicotiana suaveolens were determined during day/night cycles and found to fluctuate rhythmically between 10 and 50 nmol g(-1) fresh weight. Troughs of SAM levels were measured in the evening and night, which corresponds to the time when the major floral scent compound, methyl benzoate, is synthesized by a SAM dependent methyltransferase (NsBSMT) and when this enzyme possesses its highest activity. The SAM synthetase (NsSAMS1) and methionine synthase (NsMS1) are enzymes, among others, which are involved in the synthesis and regeneration of SAM. Respective genes were isolated from a N. suaveolens petal cDNA library. Transcript accumulation patterns of both SAM regenerating enzymes matched perfectly those of the bifunctional NsBSMT; maximum mRNA accumulations of NsMS1 and NsSAMS1 were attained in the evening. Ethylene, which is synthesized from SAM, reached only low levels of 1-2 ppbv in N. suaveolens flowers. It is emitted in a burst at the end of the life span of the flowers, which correlates with the increased expression of the 1-aminocyclopropane-1-carboxylate oxidase (NsACO).
Asunto(s)
Etilenos/metabolismo , Flores/metabolismo , Nicotiana/metabolismo , Proteínas de Plantas/metabolismo , S-Adenosilmetionina/metabolismo , 5-Metiltetrahidrofolato-Homocisteína S-Metiltransferasa/clasificación , 5-Metiltetrahidrofolato-Homocisteína S-Metiltransferasa/genética , 5-Metiltetrahidrofolato-Homocisteína S-Metiltransferasa/metabolismo , Aminoácido Oxidorreductasas/clasificación , Aminoácido Oxidorreductasas/genética , Aminoácido Oxidorreductasas/metabolismo , Northern Blotting , Clonación Molecular , ADN Complementario/química , ADN Complementario/genética , Flores/genética , Regulación del Desarrollo de la Expresión Génica , Regulación Enzimológica de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Biblioteca de Genes , Metionina Adenosiltransferasa/clasificación , Metionina Adenosiltransferasa/genética , Metionina Adenosiltransferasa/metabolismo , Datos de Secuencia Molecular , Filogenia , Proteínas de Plantas/genética , Análisis de Secuencia de ADN , Factores de Tiempo , Nicotiana/genética , Nicotiana/crecimiento & desarrolloRESUMEN
Two 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) genes have been cloned from RNA isolated from leaf tissue of apple (Malus domestica cv. Royal Gala). The genes, designated MD-ACO2 (with an ORF of 990bp) and MD-ACO3 (966bp) have been compared with a previously cloned gene of apple, MD-ACO1 (with an ORF of 942bp). MD-ACO1 and MD-ACO2 share a close nucleotide sequence identity of 93.9% in the ORF but diverge in the 3' untranslated regions (3'-UTR) (69.5%). In contrast, MD-ACO3 shares a lower sequence identity with both MD-ACO1 (78.5%) and MD-ACO2 (77.8%) in the ORF, and 68.4% (MD-ACO1) and 71% (MD-ACO2) in the 3'-UTR. Southern analysis confirmed that MD-ACO3 is encoded by a distinct gene, but the distinction between MD-ACO1 and MD-ACO2 is not as definitive. Gene expression analysis has shown that MD-ACO1 is restricted to fruit tissues, with optimal expression in ripening fruit, MD-ACO2 expression occurs more predominantly in younger fruit tissue, with some expression in young leaf tissue, while MD-ACO3 is expressed predominantly in young and mature leaf tissue, with less expression in young fruit tissue and least expression in ripening fruit. Protein accumulation studies using western analysis with specific antibodies raised to recombinant MD-ACO1 and MD-ACO3 produced in E. coli confirmed the accumulation of MD-ACO1 in mature fruit, and an absence of accumulation in leaf tissue. In contrast, MD-ACO3 accumulation occurred in younger leaf tissue, and in younger fruit tissue. Further, the expression of MD-ACO3 and accumulation of MD-ACO3 in leaf tissue is linked to fruit longevity. Analysis of the kinetic properties of the three apple ACOs using recombinant enzymes produced in E. coli revealed apparent Michaelis constants (K(m)) of 89.39 microM (MD-ACO1), 401.03 microM (MD-ACO2) and 244.5 microM (MD-ACO3) for the substrate ACC, catalytic constants (K(cat)) of 6.6x10(-2) (MD-ACO1), 3.44x10(-2) (Md-ACO2) and 9.14x10(-2) (MD-ACO3) and K(cat)/K(m) (microMs(-1)) values of 7.38x10(-4) microMs(-1) (MD-ACO1), 0.86x10(-4)Ms(-1) (MD-ACO2) and 3.8x10(-4) microMs(-1) (MD-ACO3). These results show that MD-ACO1, MD-ACO2 and MD-ACO3 are differentially expressed in apple fruit and leaf tissue, an expression pattern that is supported by some variation in kinetic properties.
Asunto(s)
Aminoácido Oxidorreductasas/genética , Aminoácido Oxidorreductasas/metabolismo , Malus/genética , Malus/metabolismo , Familia de Multigenes/fisiología , Proteínas de Plantas/metabolismo , Aminoácido Oxidorreductasas/clasificación , Southern Blotting , Western Blotting , Frutas/genética , Frutas/metabolismo , Regulación de la Expresión Génica de las Plantas/genética , Regulación de la Expresión Génica de las Plantas/fisiología , Familia de Multigenes/genética , Filogenia , Hojas de la Planta/genética , Hojas de la Planta/metabolismo , Proteínas de Plantas/clasificación , Proteínas de Plantas/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Estaciones del AñoRESUMEN
A full-length cDNA encoding D-amino acid oxidase (DAO, EC 1.4.3.3) was cloned and sequenced from the hepatopancreas of carp fed a diet supplemented with D-alanine. This clone contained an open reading frame encoding 347 amino acid residues. The deduced amino acid sequence exhibited about 60 and 19-29% identity to mammalian and microbial DAOs, respectively. The expression of full-length carp DAO cDNA in Escherichia coli resulted in a significant level of protein with DAO activity. In carp fed the diet with D-alanine for 14 days, DAO mRNA was strongly expressed in intestine followed by hepatopancreas and kidney, but not in muscle. During D-alanine administration, DAO gene was expressed quickly in hepatopancreas with the increase of DAO activity. The inducible nature of carp DAO indicates that it plays an important physiological role in metabolizing exogenous D-alanine that is abundant in their prey invertebrates, crustaceans, and mollusks.
Asunto(s)
Alanina/administración & dosificación , Carpas/genética , Carpas/metabolismo , D-Aminoácido Oxidasa/química , D-Aminoácido Oxidasa/metabolismo , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Alineación de Secuencia , Administración Oral , Aminoácido Oxidorreductasas/química , Aminoácido Oxidorreductasas/clasificación , Aminoácido Oxidorreductasas/genética , Aminoácido Oxidorreductasas/metabolismo , Secuencia de Aminoácidos , Animales , Clonación Molecular , Secuencia Conservada , D-Aminoácido Oxidasa/clasificación , D-Aminoácido Oxidasa/genética , D-Aspartato Oxidasa , Relación Dosis-Respuesta a Droga , Activación Enzimática , Hepatopáncreas/química , Hepatopáncreas/efectos de los fármacos , Hepatopáncreas/enzimología , Humanos , Ratones , Datos de Secuencia Molecular , Especificidad de Órganos , Proteínas Recombinantes/química , Proteínas Recombinantes/clasificación , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Análisis de Secuencia de Proteína , Homología de Secuencia de Aminoácido , Especificidad de la Especie , PorcinosRESUMEN
Fructosyl peptide oxidases, enzymes that are active against a model compound of glycated hemoglobin, N(alpha)-fructosyl valyl-histidine, were characterized. To identify the primary structure of fructosyl peptide oxidases, we have prepared cDNA libraries from Eupenicillium terrenum ATCC18547 and Coniochaeta sp. NISL9330. The coding regions, both fungal fructosyl peptide oxidases consisting of 1314-bp, were obtained with degenerated primers based on the amino acid sequences and specific primers by 3(') and 5(') RACE (rapid amplification of cDNA ends). By their sequence similarities and substrate specificities, fructosyl peptide oxidases and their homologs could be categorized into two groups: (A) enzymes that preferably oxidize alpha-glycated molecules and (B) enzymes that preferably oxidize epsilon-glycated molecules. We showed that recombinant fructosyl peptide oxidases could be used to detect protease-treated fructosyl-hexapeptide, a glycated peptide that is released from HbA(1C) by endoproteinase Glu-C, suggesting these enzymes could be useful for the enzymatic measurement of HbA(1C).
Asunto(s)
Aminoácido Oxidorreductasas/biosíntesis , Aminoácido Oxidorreductasas/química , Ascomicetos/química , Ascomicetos/enzimología , Hemoglobina Glucada/análisis , Hemoglobina Glucada/química , Técnicas de Sonda Molecular , Aminoácido Oxidorreductasas/clasificación , Aminoácido Oxidorreductasas/aislamiento & purificación , Secuencia de Aminoácidos , Ascomicetos/clasificación , Clonación Molecular , Activación Enzimática , Concentración de Iones de Hidrógeno , Datos de Secuencia Molecular , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Homología de Secuencia , Especificidad por SustratoRESUMEN
We have identified a novel 14-exon human lysyl oxidase-like gene, LOXL4, on chromosome 10q24. The cDNA and derived amino acid sequence of LOXL4 demonstrates a conserved C-terminal region including the characteristic copper-binding site, lysyl and tyrosyl residues and a cytokine receptor-like domain. One of the four N-terminal SRCR domains contains a 13 amino acid insertion encoded by a short exon not present within the closely homologous LOXL2 and LOXL3 genes. The 3.5-kb LOXL4 mRNA is present in pancreas and testis and at lower levels in several other tissues. Fibroblasts, smooth muscle and osteosarcoma (HOS) cells express LOXL4. No expression was detected in HCT-116 and DLD-1 colon, MCF-7 breast and DU-145 prostate cancer cell lines.
Asunto(s)
Aminoácido Oxidorreductasas/genética , Cromosomas Humanos Par 10 , Cisteína/metabolismo , Proteínas de la Membrana , Receptores de Lipoproteína , Regiones no Traducidas 3' , Regiones no Traducidas 5' , Aminoácido Oxidorreductasas/clasificación , Aminoácido Oxidorreductasas/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Sitios de Unión , Cisteína/genética , ADN Complementario , Exones , Humanos , Masculino , Ratones , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Estructura Terciaria de Proteína , Proteína-Lisina 6-Oxidasa , Receptores Inmunológicos/genética , Receptores Inmunológicos/metabolismo , Receptores Depuradores , Receptores Depuradores de Clase B , Distribución TisularRESUMEN
We report the complete cDNA sequence of the human lysyl oxidase-like 4 (LOXL4) gene, a new member of the lysyl oxidase (LO) gene family. The predicted polypeptide is 756 amino acids long, including a 24-residue signal peptide. The C-terminal region contains a LO domain similar to those of LOX, LOXL, LOXL2 and LOXL3. The N-terminal region has four subregions similar to scavenger receptor cysteine-rich domains that are highly conserved with LOXL2 and LOXL3. The LOXL4 mRNA is approximately 4 kb in size and is expressed in many tissues, the highest levels among the tissues studied being in the skeletal muscle, testis and pancreas. Recombinant LOXL4 expressed in HT-1080 cells was secreted into the culture medium with no evident proteolytic processing.
Asunto(s)
Aminoácido Oxidorreductasas/genética , Cromosomas Humanos Par 10 , Cisteína/genética , Proteínas de la Membrana , Receptores de Lipoproteína , Aminoácido Oxidorreductasas/clasificación , Aminoácido Oxidorreductasas/metabolismo , Secuencia de Aminoácidos , Animales , Clonación Molecular , Cisteína/metabolismo , Humanos , Isoenzimas/clasificación , Isoenzimas/genética , Masculino , Ratones , Datos de Secuencia Molecular , Péptidos/clasificación , Péptidos/genética , Estructura Terciaria de Proteína , Proteína-Lisina 6-Oxidasa , Receptores Inmunológicos/genética , Receptores Inmunológicos/metabolismo , Receptores Depuradores , Receptores Depuradores de Clase B , Homología de Secuencia de Aminoácido , Distribución Tisular , Células Tumorales CultivadasRESUMEN
Nitric oxide (NO) is synthesized in skeletal muscle by neuronal-type NO synthase (nNOS), which is localized to sarcolemma of fast-twitch fibers. Synthesis of NO in active muscle opposes contractile force. We show that nNOS partitions with skeletal muscle membranes owing to association of nNOS with dystrophin, the protein mutated in Duchenne muscular dystrophy (DMD). The dystrophin complex interacts with an N-terminal domain of nNOS that contains a GLGF motif. mdx mice and humans with DMD evince a selective loss of nNOS protein and catalytic activity from muscle membranes, demonstrating a novel role for dystrophin in localizing a signaling enzyme to the myocyte sarcolemma. Aberrant regulation of nNOS may contribute to preferential degeneration of fast-twitch muscle fibers in DMD.
Asunto(s)
Aminoácido Oxidorreductasas/análisis , Distrofina/análisis , Músculo Esquelético/química , Distrofias Musculares/enzimología , Sarcolema/química , Aminoácido Oxidorreductasas/clasificación , Secuencia de Aminoácidos , Animales , Citoesqueleto/química , Citosol/enzimología , Humanos , Inmunohistoquímica , Isoenzimas/análisis , Ratones , Ratones Endogámicos mdx , Datos de Secuencia Molecular , Músculo Esquelético/enzimología , Distrofia Muscular Animal/enzimología , Óxido Nítrico Sintasa , Sarcolema/enzimologíaRESUMEN
In the adult central nervous system, nitric oxide (NO) is formed from L-arginine by the so-called constitutive or type I NO synthase (NOS-I155). However, expression of NOS-I155 immunoreactivity and activity was low or not detectable in developing mouse and rat brain. NOS-I155 was sharply induced coincident with the onset of synaptogenesis in specific brain regions. This was followed by a second phase in which total NOS-I155 expression decreased both in specific cell populations and in the total synaptosomal subcellular fraction.Furthermore, two putative variants of NOS-I were transiently observed: an NOS-I-immunoreactive protein with increased electrophoretic mobility (NOS-I144) and a transient hypersensitivity of NOS-I155 to the competitive substrate inhibitor N omega-nitro-L-arginine. It is concluded that NOS-I expression is not constitutive but locally induced. In the central nervous system, this regionally specific, biphasic pattern of postnatal NOS-I induction is consistent with a role for NO in synaptogenesis and synaptic plasticity.
Asunto(s)
Aminoácido Oxidorreductasas/biosíntesis , Encéfalo/embriología , Encéfalo/crecimiento & desarrollo , Neuronas/enzimología , Sinapsis/enzimología , Aminoácido Oxidorreductasas/clasificación , Animales , Secuencia de Bases , Western Blotting , Encéfalo/enzimología , Fraccionamiento Celular , Inducción Enzimática , Femenino , Inmunohistoquímica , Masculino , Ratones , Ratones Endogámicos C57BL , Datos de Secuencia Molecular , Óxido Nítrico Sintasa , Reacción en Cadena de la Polimerasa , Empalme del ARN , ARN Mensajero/análisis , ARN Mensajero/genética , Ratas , Ratas Sprague-DawleyRESUMEN
Nitric oxide (NO), which accounts for the biological activity of endothelium-derived relaxing factor, is now thought to play a variety of roles in the nervous system and in immunologic reactions. NO is synthesized from L-arginine by nitric oxide synthase (NOS). There are three isoforms of NOS; type I (neuronal), type II (inducible), and type III (endothelial). The fundamental structure of the three isoforms, which contain calmodulin-, FMN-, FAD-, and NADPH-binding domains, is the same. Calmodulin is already bound to inducible NOS without requiring Ca2+, while the others are Ca2+/calmodulin-dependent. Endothelial NOS is bound to membranes by N-myristoylation, while the other isoforms are soluble. The human endothelial NOS gene has been cloned. It has several highly repetitive regions which could provide potential sites for DNA polymorphism. It might be of interest to examine the relationship between such polymorphism and cardiovascular disorders.
Asunto(s)
Aminoácido Oxidorreductasas/genética , Polimorfismo Genético , Aminoácido Oxidorreductasas/clasificación , Aminoácido Oxidorreductasas/fisiología , Endotelio Vascular/fisiología , Humanos , Óxido Nítrico/fisiología , Óxido Nítrico Sintasa , Relación Estructura-ActividadAsunto(s)
Aminoácido Oxidorreductasas/metabolismo , Regulación Enzimológica de la Expresión Génica , Aminoácido Oxidorreductasas/biosíntesis , Aminoácido Oxidorreductasas/clasificación , Aminoácido Oxidorreductasas/genética , Isoenzimas/metabolismo , Modelos Genéticos , Óxido Nítrico Sintasa , Procesamiento Proteico-Postraduccional , Procesamiento Postranscripcional del ARN , Transcripción GenéticaRESUMEN
L-Phenylalanine dehydrogenase catalyzes the NAD(+)-dependent, reversible, oxidative deamination of L-phenylalanine to form ammonia, phenyl pyruvate, and NADH. The enzyme has been purified to homogeneity from Rhodococcus sp. M4, and a partial amino acid sequence was obtained. A cosmid library of Rhodococcus sp. M4 genomic DNA was prepared and used to isolate a 2.5-kilobase PstI fragment that contained the pdh gene. The open reading frame of 1068 nucleotides encodes a polypeptide of 356 amino acids, portions of which match the amino acid sequence determined for the purified enzyme. Expression of the Rhodococcus pdh gene in Escherichia coli, which does not contain a phenylalanine dehydrogenase activity, yields a soluble enzyme exhibiting phenylalanine dehydrogenase activity. Both the enzyme purified from Rhodococcus and the enzyme expressed in E. coli are post-translationally modified by removal of the amino-terminal methionine. The overall amino acid sequence is homologous to previously reported sequences of leucine and phenylalanine dehydrogenases as well as several glutamate dehydrogenases. The amino-terminal portion of the enzyme contains residues involved in L-amino acid binding and catalysis, while the carboxyl-terminal portion contains the presumptive dinucleotide-binding domain. A detailed sequence comparison of Rhodococcus phenylalanine dehydrogenase with leucine, phenylalanine, and glutamate dehydrogenases suggests residues involved in general amino acid binding and others that provide for amino acid discrimination.
Asunto(s)
Aminoácido Oxidorreductasas/genética , Genes Bacterianos/genética , Rhodococcus/genética , Aminoácido Oxidorreductasas/clasificación , Aminoácido Oxidorreductasas/aislamiento & purificación , Secuencia de Aminoácidos , Aminoácidos/análisis , Secuencia de Bases , Evolución Biológica , Clonación Molecular , Biblioteca Genómica , Geobacillus stearothermophilus/enzimología , Geobacillus stearothermophilus/genética , Leucina-Deshidrogenasa , Datos de Secuencia Molecular , Rhodococcus/enzimología , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Especificidad por SustratoRESUMEN
The expression of mRNA for the calmodulin-dependent form of brain nitric oxide synthase (NOS) was examined in cholinergic cells of the rat brain using a method combining in situ hybridization histochemistry with immunocytochemistry for choline acetyltransferase (ChAT) in the same brain sections. We constructed a riboprobe specific for brain NOS by subcloning a 493 bp fragment of the coding region which displayed low homology to other forms of NOS. The general distribution of NOS mRNA was in excellent agreement with previous studies using the full-length probe or NADPH diaphorase histochemistry. NOS mRNA was observed in many brain structures and relative levels were quantitated using grain counting procedures in a number of cholinergic and non-cholinergic neuronal groups throughout the brain. In the forebrain, ChAT-immunoreactive cells or cell groups were observed in medial septum (MS), vertical limbs of diagonal band (DBV) and horizontal limbs of diagonal band (DBH), nucleus basalis magnocellularis (NBM), substantia innominata (SI), and striatum (ST). In the brainstem, the cholinergic groups studied included those located in the pedunculopontine tegmental nucleus (PPTN), the laterodorsal tegmental nucleus (LDTN), the nucleus parabigeminalis and several motor nuclei. For NOS mRNA quantitation, silver grains overlying ChAT-stained neuronal profiles in sections on emulsion-dipped slides were counted digitally. In the LDTN and PPTN, virtually all the ChAT-positive cells expressed NOS mRNA at high levels. In MS, DBV and SI, about 30-50% of the ChAT-positive cells expressed NOS mRNA at low-to-moderate levels. Less than 20% of ChAT-positive neurons in the other cholinergic populations studied expressed NOS mRNA; the NBM was one of these low-expressing populations. Many scattered non-cholinergic cells expressing NOS mRNA were found in the striatum and cerebral cortex. In other non-cholinergic regions, high NOS mRNA expression was observed in the islands of Calleja, thalamic and hypothalamic nuclei, several amygdaloid nuclei, regions related to the optic tract, the interpeduncular nucleus, and the supramammillary nucleus. The heterogeneous distribution of NOS mRNA implies complex roles for nitric oxide neurotransmission in brain function, including for the cholinergic phenotype. Additionally, given the postulated involvement of nitric oxide in neurodegeneration, the widely varying levels of expression of NOS within identified central cholinergic neurons may relate to differential vulnerability of this phenotype in disease or aging.
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Aminoácido Oxidorreductasas/biosíntesis , Encéfalo/enzimología , Fibras Colinérgicas/enzimología , Proteínas del Tejido Nervioso/biosíntesis , Aminoácido Oxidorreductasas/clasificación , Aminoácido Oxidorreductasas/genética , Animales , Autorradiografía , Biomarcadores/análisis , Encéfalo/citología , Calmodulina , Colina O-Acetiltransferasa/análisis , Procesamiento de Imagen Asistido por Computador , Técnicas para Inmunoenzimas , Hibridación in Situ , Masculino , NADP , Proteínas del Tejido Nervioso/genética , Óxido Nítrico Sintasa , Ratas , Ratas Sprague-DawleyRESUMEN
Porcine kidney tubular epithelial cells (LLC-PK1) produce nitric oxide or a related compound (e.g., a nitrosothiol) after stimulation with various agonists. We now report the identification and characterization of a constitutive, particulate nitric oxide (NO) synthase from LLC-PK1 cells. After partial purification on adenosine 2',5'-bisphosphate-Sepharose, the particulate NO synthase activity eluted anomalously from Superose 6 gel permeation columns near the total included volume, similar to that observed for the endothelial (type III) NO synthase. Substrate/cofactor requirements of the epithelial and endothelial NO synthases were identical, i.e., dependency on L-arginine, (6R)-5,6,7,8-tetrahydrobiopterin, FAD, calcium and calmodulin. The epithelial enzyme activity was inhibited by the arginine analogues, NG-methyl-L-arginine (100 microM) and NG-nitro-L-arginine (100 microM), as well as the calmodulin antagonists, trifluoperazine (100 microM) and calmidazolium (30 microM). Anti-type III (H32), but not anti-type I (brain, 6763-5) or anti-type II (macrophage, 8196) NO synthase antibodies, detected a single immunoreactive band in the LLC-PK1 particulate fraction of approximately 140 kDa by Western blot analysis. Finally, the presence of type III NO synthase mRNA in LLC-PK1 cells was demonstrated using the polymerase chain reaction. These data indicate that LLC-PK1 kidney epithelial cells contain type III NO synthase, which has been classically associated with the vascular endothelium.
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Aminoácido Oxidorreductasas/metabolismo , Endotelio Vascular/enzimología , Isoenzimas/metabolismo , Riñón/enzimología , Aminoácido Oxidorreductasas/clasificación , Animales , Secuencia de Bases , Línea Celular , Células Epiteliales , Epitelio/enzimología , Riñón/citología , Datos de Secuencia Molecular , Óxido Nítrico Sintasa , Sondas de Oligonucleótidos/genética , Reacción en Cadena de la Polimerasa , ARN Mensajero/metabolismo , PorcinosRESUMEN
The present study was aimed at determining chronological alterations of Ca2+/calmodulin (CaM)-dependent and -independent nitric oxide synthase (NOS) activities in brain microvessels (MV) isolated from the affected hemisphere following an occlusion of the middle cerebral artery (MCAo) in rats. It was shown that significant enhancements of Ca2+/CaM-independent NOS activity to 922% and 920% of control level were manifested at 4 h and 24 h, respectively, which returned to the control level at 48 h after MCAo. Regarding Ca2+/CaM-dependent NOS, on the other hand, it was shown that the activity was invariably increased to 374% and 743% of control level at 48 h and 1 week following MCAo, respectively. Thus, the present study provided the first evidence that two distinct types of NOS activities were increased with different temporal patterns after MCAo. These heterogeneous alterations of NOS activities may be of critical importance for the induction of brain damage following cerebral ischemia.
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Aminoácido Oxidorreductasas/fisiología , Daño Encefálico Crónico/fisiopatología , Edema Encefálico/fisiopatología , Isquemia Encefálica/fisiopatología , Encéfalo/irrigación sanguínea , Calcio/fisiología , Calmodulina/fisiología , Aminoácido Oxidorreductasas/clasificación , Animales , Masculino , Microcirculación/fisiopatología , Óxido Nítrico Sintasa , Ratas , Ratas Sprague-DawleyRESUMEN
Nitric oxide (NO) has been identified recently as a multifunctional mediator, produced by, and acting on, most cells of the body. Besides its function as endothelium-derived relaxing factor, as a neurotransmitter and as an immune defence molecule, evidence is accumulating that NO participates in inflammatory- and autoimmune-mediated tissue destruction. Modulation of NO synthesis and action represents a new approach to the treatment of inflammatory and autoimmune conditions.