RESUMEN
To improve the availability of three-dimensional (3D) structures of HLA molecules, we created the pHLA3D database. In its first version, we modeled and published 106 3D structures of HLA class I molecules from the HLA-A, HLA-B, and HLA-C loci. This paper presents an update of this database, providing more 127 3D structures of HLA class II molecules (41 DR, 42 DQ, and 44 DP), predicted via homology modeling with MODELLER and SWISS-MODEL. These new 3D structures of HLA class II molecules are now freely available at pHLA3D (www.phla3d.com.br) for immunologists and other researchers working with HLA molecules.
Asunto(s)
Antígenos HLA-DP/ultraestructura , Antígenos HLA-DQ/ultraestructura , Antígenos HLA-DR/ultraestructura , Biología Computacional , Bases de Datos de Proteínas , Humanos , Estructura Terciaria de Proteína , Homología de Secuencia de Aminoácido , Programas InformáticosRESUMEN
BACKGROUND: Expression of HLA-DP and HLA-DQ antigens on the microvasculature in normal skin is uncertain. OBJECTIVE AND METHODS: We investigated expression of HLA-DR, HLA-DP, and HLA-DQ antigens on the microvasculature in normal skin by light and electron microscopic immunohistochemistry. RESULTS: HLA-DP and HLA-DQ antigens were expressed on the microvasculature, but the intensity of positiveness was variable. By immunoelectron microscopy, HLA-DR, HLA-DP, and HLA-DQ antigens were expressed on the endothelial cells of the microvasculature, but not on the pericytes and the smooth muscle cells. The luminal surface of endothelial cells had a stronger expression of these antigens than the abluminal surface. CONCLUSION: HLA-DR, HLA-DP, and HLA-DQ antigens are expressed on endothelial cells of the microvasculature in normal skin.
