RESUMEN
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Asunto(s)
Humanos , Masculino , Lactante , Mastoiditis/diagnóstico , Periostio/microbiología , Mastoiditis/diagnóstico por imagen , Mastoiditis/tratamiento farmacológico , Enfermedades Óseas Infecciosas , Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/patogenicidadRESUMEN
BACKGROUND AND OBJECTIVE: The purpose of this study was to determine the time of infection by anaerobic gram-negative rods associated with periodontal disease, and to clarify their transmission from mother to child. MATERIAL AND METHODS: Seventy-eight Japanese children (including 10 siblings), aged from 3 to 9 years, and 68 mothers, were enrolled in this study. Colonization by 11 periodontal bacterial species was determined using polymerase chain reaction amplification of samples of subgingival plaque obtained from the children and their mothers. RESULTS: The detection rates of Porphyromonas gingivalis, Tannerella forsythensis and Treponema denticola increased in children after the age of 6 years. We found a high consistency in colonization by P. gingivalis, T. denticola, Prevotella intermedia and Prevotella nigrescens in 9 of the 10 siblings. The average number of bacterial species in plaque samples harboring Fusobacterium nucleatum and/or Fusobacterium periodonticum was significantly greater than in those without, in both children and mothers. Kappa statistical analysis revealed that the detection of Capnocytophaga gingivalis, Capnocytophaga ochracea, Campylobacter rectus and T. denticola in children was consistent with that in the mother. CONCLUSION: Periodontal bacterial colonization in Japanese children increased with age and was associated with F. nucleatum and/or periodonticum, and the bacterial flora in children was similar to that in their mothers.
Asunto(s)
Placa Dental/microbiología , Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/patogenicidad , Infecciones por Bacterias Gramnegativas/transmisión , Transmisión Vertical de Enfermedad Infecciosa , Periodontitis/microbiología , Factores de Edad , Bacteroides/patogenicidad , Bacteroides/fisiología , Niño , Preescolar , Femenino , Fusobacterium/patogenicidad , Fusobacterium/fisiología , Infecciones por Fusobacterium/transmisión , Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/aislamiento & purificación , Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/fisiología , Humanos , Japón , Madres , Reacción en Cadena de la PolimerasaRESUMEN
OBJECTIVE: To determine the bacterial species associated with spreading odontogenic infections (SOIs). STUDY DESIGN: Pus samples from 4 cases of SOI were analyzed by microbiological culture methods for the presence of bacteria, and by polymerase chain reaction (PCR) amplification, cloning, and sequencing of bacterial 16S rRNA genes. RESULTS: Culture methods identified species from the genera Prevotella, Streptococcus, and Fusobacterium, as well as anaerobic streptococci. Molecular detection methods identified a far more diverse microflora. The predominant genus detected was Prevotella, representing 102 (50.2%) of 203 clones analyzed. Prevotella oris was the most abundant species identified, representing 45 (22.2%) of 203 clones analyzed. Twelve clones (5.9%) represented uncultivable species, namely Prevotella PUS9.180, an uncultured Peptostreptococcus species, and an uncultured bacterium belonging to the Bacteroidetes phylum. CONCLUSIONS: Prevotella species may play an important role in SOIs, and further work to examine in more detail the pathogenicity determinants of these organisms and associated host responses is warranted.
Asunto(s)
Técnicas de Tipificación Bacteriana , Infección Focal Dental/microbiología , Prevotella/patogenicidad , Adolescente , Adulto , Recuento de Colonia Microbiana , ADN Bacteriano/análisis , Femenino , Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/patogenicidad , Humanos , Masculino , Reacción en Cadena de la Polimerasa , Porphyromonas/patogenicidad , ARN Ribosómico 16S/análisis , Análisis de Secuencia de ADN , Supuración/microbiologíaRESUMEN
OBJECTIVE: This study intended to examine samples from primary endodontic infections for the presence of Catonella morbi and Granulicatella adiacens, 2 species that have been recently suggested to be involved with infections in other oral sites. STUDY DESIGN: Genomic DNA was isolated directly from samples taken from teeth with different forms of apical periodontitis, and a devised culture-independent 16S rRNA gene-based heminested PCR assay was used to determine the prevalence of these 2 target species. Species-specific primers were developed by aligning closely related 16S rRNA gene sequences. Species-specificity for each primer pair was confirmed by running PCR against a panel of oral bacteria and by sequencing of DNA from representative positive samples. RESULTS: C morbi and G adiacens were detected in 33% and 19%, respectively, of the root canals associated with chronic apical periodontitis; 30% and 10%, respectively, of the cases diagnosed as acute apical periodontitis, and 16% and 11%, respectively, of the pus samples taken from acute apical abscesses. Overall, C morbi occurred in 26% and G adiacens in 14% of the samples taken from primary endodontic infections. CONCLUSIONS: Our findings demonstrate that C morbi and G adiacens can take part in the microbiota associated with primary endodontic infections, and their specific role in the disease process warrants further elucidation.
Asunto(s)
Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/patogenicidad , Periodontitis Periapical/microbiología , Streptococcus/patogenicidad , Adolescente , Adulto , ADN Bacteriano/análisis , Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/aislamiento & purificación , Infecciones por Bacterias Gramnegativas/microbiología , Humanos , Persona de Mediana Edad , Absceso Periapical/microbiología , Reacción en Cadena de la Polimerasa/métodos , Ribotipificación , Análisis de Secuencia de ADN , Infecciones Estreptocócicas/microbiología , Streptococcus/aislamiento & purificaciónRESUMEN
The objective of this study was to quantitatively compare the bacterial population structure in plaque from the gingival margin of two groups of 21 Chinese patients with gingivitis or necrotizing ulcerative gingivitis (NUG). Subjects were recruited in four dental clinics in Eastern China. Samples were quantitatively assessed by immunofluorescence and fluorescent in situ hybridization for taxa known to be associated with periodontal diseases. The analyses showed that the fusiform taxa (Fusobacterium nucleatum/Fusobacterium periodonticum, Leptotrichia buccalis, Tannerella forsythensis, and Capnocytophaga sp.), Campylobacter rectus, Prevotella intermedia, Prevotella nigrescens, Selenomonas sputigena, and treponemes were present in both groups with high prevalence. Porphyromonas gingivalis and Actinomyces gerencseriae were much more prevalent in the NUG group. Quantitatively, most taxa, including P. gingivalis, F. nucleatum and the treponemes, accounted, on average, for < 3% of the total bacterial cell number. Only P. intermedia/P. nigrescens, P. gingivalis, S. sputigena, A. gerencseriae, and the sum of all monitored suspected periodontal pathogens were significantly increased in the NUG group. The present study demonstrates for both groups a highly diverse plaque composition and suggests that, etiologically, the overall concentration and the concerted effects of the entire group of opportunistic pathogens thriving in NUG-associated plaque are of prime importance.
Asunto(s)
Gingivitis Ulcerosa Necrotizante/microbiología , Adulto , Técnicas de Tipificación Bacteriana , China , Recuento de Colonia Microbiana , Placa Dental/microbiología , Femenino , Técnica del Anticuerpo Fluorescente , Líquido del Surco Gingival/microbiología , Gingivitis/microbiología , Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/aislamiento & purificación , Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/patogenicidad , Humanos , Hibridación Fluorescente in Situ , Masculino , Persona de Mediana Edad , Estadísticas no Paramétricas , Treponema/aislamiento & purificación , Treponema/patogenicidadRESUMEN
Dialister pneumosintes is an anaerobic Gram-negative rod that has been recently implicated as a candidate endodontic pathogen. In this study, samples taken from abscessed teeth and infected root canals associated with asymptomatic or symptomatic periradicular lesions were examined for the occurrence of bacterial associations involving D. pneumosintes. DNA was extracted from the samples, and the presence of D. pneumosintes and 16 other bacterial species was determined by means of species-specific nested polymerase chain reaction. Positive and negative associations involving D. pneumosintes were investigated by computing the odds ratio of D. pneumosintes being found in a sample from endodontic infection in co-infection with one of the other target species. The association between the pairs containing D. pneumosintes and the occurrence of pain also was evaluated. D. pneumosintes was always detected in mixed infections with at least two of the other target species. D. pneumosintes was positively associated with Treponema denticola, Porphyromonas endodontalis, Fusobacterium nucleatum, Peptostreptococcus micros, Campylobacter rectus, Prevotella intermedia, T. pectinovorum, and T. vincentii. Negative associations were observed with Bacteroides forsythus, P. gingivalis, and Actinomyces israelii. No pair containing D. pneumosintes was found to be significantly associated with symptomatic cases (p > 0.01). The findings of this study lend considerable support to the notion of D. pneumosintes being an important endodontic pathogen, usually in a mixed infection. Positive associations of this species with other highly prevalent species, such as T. denticola and P. endodontalis, suggest that bacterial synergism can occur and thereby play an important role in the pathogenesis of different forms of periradicular lesions.
Asunto(s)
Necrosis de la Pulpa Dental/microbiología , Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/patogenicidad , Infecciones por Bacterias Gramnegativas/microbiología , Periodontitis Periapical/microbiología , Adolescente , Adulto , Técnicas de Tipificación Bacteriana , ADN Bacteriano/genética , ADN Ribosómico/genética , Humanos , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa , SimbiosisRESUMEN
Destructive periodontal disease is associated with human cytomegalovirus (HCMV), Epstein-Barr type 1 virus (EBV-1) and other members of the Herpesviridae family as well as with various gram-negative anaerobic bacteria, including the Dialister pneumosintes species. This study aimed to determine possible interrelationships between periodontal HCMV, EBV-1, herpes simplex virus and D. pneumosintes, and relate the microbiological findings to periodontitis clinical status. Sixteen subjects each contributed paper point samples from two progressing and two stable periodontitis lesions, as determined by ongoing loss of probing attachment. Polymerase chain reaction methodology was used to identify the study herpesviruses and D. pneumosintes. Chi-squared tests, Fisher exact tests and multivariate logistic regression were employed to identify statistical associations among herpesviruses, bacteria and clinical variables. HCMV, and no other virus or combination of viruses, was positively associated with the presence of D. pneumosintes, and the relationship was specific for individual periodontitis sites with no detectable subject effect. D. pneumosintes was in turn positively associated with periodontal pocket depth and disease-active periodontitis. When the average percentage of alveolar bone loss in all teeth was treated as a response, HCMV remained significant even after D. pneumosintes was included in the model, suggesting that both HCMV and D. pneumosintes affected bone loss or, alternatively, HCMV affected factors not studied that themselves can induce bone loss. We hypothesize that periodontal HCMV sets the stage for subgingival proliferation of D. pneumosintes and subsequent periodontal disease progression. Studies on herpesviral-bacterial interactions may hold great promise for delineating important etio-pathogenic aspects of destructive periodontal disease.
Asunto(s)
Pérdida de Hueso Alveolar/microbiología , Citomegalovirus/patogenicidad , Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/patogenicidad , Infecciones por Bacterias Gramnegativas/microbiología , Periodontitis/microbiología , Adulto , Pérdida de Hueso Alveolar/virología , Análisis de Varianza , Distribución de Chi-Cuadrado , Citomegalovirus/aislamiento & purificación , Placa Dental/microbiología , Placa Dental/virología , Progresión de la Enfermedad , Ecología , Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/aislamiento & purificación , Herpesvirus Humano 4/aislamiento & purificación , Herpesvirus Humano 4/patogenicidad , Humanos , Modelos Logísticos , Análisis Multivariante , Pérdida de la Inserción Periodontal/microbiología , Pérdida de la Inserción Periodontal/virología , Bolsa Periodontal/microbiología , Bolsa Periodontal/virología , Periodontitis/virología , Reacción en Cadena de la Polimerasa , Simplexvirus/aislamiento & purificación , Simplexvirus/patogenicidad , Estadística como AsuntoRESUMEN
OBJECTIVE: Dialister pneumosintes is a nonmotile, nonfermentative, non-spore-forming, obligately anaerobic, gram-negative bacillus that has been associated with some infections in the human body. A species-specific nested polymerase chain reaction assay was used to investigate the occurrence of D pneumosintes in primary root canal infections. STUDY DESIGN: Samples were collected from 32 teeth with carious lesions, necrotic pulps, and radiographic evidence of periradicular bone destruction. Twenty-two teeth were asymptomatic, and 10 cases were diagnosed as acute apical periodontitis. DNA extracted from the samples was initially amplified with universal 16S ribosomal DNA primers. A second round of amplification used the first polymerase chain reaction products to specifically detect D pneumosintes. RESULTS: This bacterial species was detected in 17 of 22 asymptomatic cases (77.3%) and in 4 of 10 root canals associated with acute apical periodontitis (40%). No relationship was found between the presence of this bacterial species and the occurrence of symptoms. In general, D pneumosintes was detected in 21 of 32 root canal samples (65.6%). CONCLUSION: This study is the first to report a high prevalence of D pneumosintes in root canal infections of humans. Because of this high prevalence and the apparent pathogenicity of the microorganism, we suggest the inclusion of D pneumosintes in the selected group of putative endodontic pathogens.
Asunto(s)
Cavidad Pulpar/microbiología , Enfermedades de la Pulpa Dental/microbiología , Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/clasificación , Infecciones por Bacterias Gramnegativas/diagnóstico , Enfermedad Aguda , Adolescente , Adulto , Distribución de Chi-Cuadrado , ADN Bacteriano/genética , Caries Dental/microbiología , Necrosis de la Pulpa Dental/microbiología , Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/genética , Bacilos Gramnegativos Anaerobios Rectos, Curvos y Espirales/patogenicidad , Humanos , Persona de Mediana Edad , Periodontitis Periapical/microbiología , Reacción en Cadena de la Polimerasa , VirulenciaRESUMEN
Although high success rates for root-form endosseous implants have been reported, failures occasionally occur, and these implants must be removed. At least 10% of the failures have been suggested to be the result of peri-implantitis. There is some evidence that periodontal pathogens, mainly those belonging to the group of gram-negative anaerobic rods, play a role in the etiology of peri-implantitis. This article provides an overview of the literature associated with common peri-implant microbiology and an assessment as to whether bacteria associated with periodontitis exert a possible risk for peri-implant tissue breakdown. The peri-implant area is colonized by a large variety of oral microbial complexes. The microflora of the oral cavity prior to implant placement determines the composition of the microflora in the peri-implant area. Implants involved in peri-implantitis are colonized with large amounts of gram-negative anaerobic bacteria, including Fusobacteria, spirochetes, Bacteroides forsythus, and "black-pigmented bacteria" such as Prevotella intermedia, Prevotella nigrescens, and Porphyromonas gingivalis. Also, Actinobacillus actinomycetemcomitans can be isolated from these lesions. Thus, the microflora of peri-implantitis lesions resembles that of adult or refractory periodontitis. However, the presence of periodontal pathogens does not always lead to a destructive process. Therefore, the etiologic role of specific microorganisms in implant failure related to infection is still not resolved. Controversy remains as to whether organisms recovered from the original microflora cause the failure (and if so to what extent) or merely result from the infection. Nevertheless, there is accumulating evidence that bacteria cause the disease, while the individual's genetic makeup and environmental influences determine the severity of the disease.