RESUMEN
Periodontitis is a public health issue, being one of the most prevalent diseases worldwide. However, the aetiology of the disease is still unclear: genetics of patients cannot explain the dispersed or isolated localisation of gingival pockets, while bacteria-based models are insufficient to distinguish gingivitis and periodontitis. The possible role of parasites in the establishment of periodontitis has been poorly studied until now. The aim of this project was to study a potential link between colonisation of gingival crevices by the amoeba Entamoeba gingivalis and periodontitis. In eight different dental clinics in France, samples were taken in periodontal pockets (72) or healthy sites (33), and submitted to microscopic observation and molecular identification by PCR with a new set of primers designed to specifically detect E. gingivalis. This blind sample analysis showed the strong sensitivity of PCR compared with clinical diagnosis (58/72 = 81%), and microscopy (51/65 = 78%). The results of this work show that the parasites detected by microscopy mainly - if not exclusively - belong to the species E. gingivalis and that the presence of the parasite is correlated with periodontitis.
Asunto(s)
Entamoeba/aislamiento & purificación , Entamebiasis/parasitología , Bolsa Periodontal/parasitología , Secuencia de Bases , Cartilla de ADN , ADN Protozoario/genética , ADN Ribosómico/genética , Entamoeba/genética , Entamoeba/patogenicidad , Entamebiasis/epidemiología , Francia/epidemiología , Hemorragia Gingival/epidemiología , Humanos , Microscopía , Datos de Secuencia Molecular , Bolsa Periodontal/epidemiología , Reacción en Cadena de la Polimerasa , Prevalencia , ARN Ribosómico/genética , Ribotipificación , Sensibilidad y Especificidad , Alineación de Secuencia , Homología de Secuencia de Ácido Nucleico , Método Simple CiegoRESUMEN
Human myiasis is characterized by larval infestation of body tissues or cavities of live individuals. Although most frequently observed in underdeveloped and tropical countries, reports of human myiasis have been encountered throughout the world. Conventional treatment is the mechanical removal of the larvae, one by one, which is painful and embarrassing and repugnant to both health professional and patient. Even though human myiasis is not considered rare, most dental professionals have very little knowledge of the diagnosis and treatment of this pathology. The purpose of this article, therefore, is to report a case of advanced-stage oral myiasis in a 9-year-old patient treated at a health and dentistry clinic. Diagnosis was based on visual observation of Cochliomyia hominivorax larvae, a dipterous fly of the Calliphoridae family, which were between the second and third stages of development. Predisposition factors include lack of information, malnutrition, poor oral hygiene, preexistence of oral lesions, and severe halitosis, which decisively influence the appearance and development of oral myiasis. A brief review of the literature is also included, in which the importance of early clinical diagnosis, myiasis etiology, and possible associations with other pathologies and different types of treatment are discussed.
Asunto(s)
Enfermedades de las Encías/parasitología , Miasis/diagnóstico , Animales , Niño , Dípteros/clasificación , Estudios de Seguimiento , Hemorragia Gingival/parasitología , Hiperplasia Gingival/parasitología , Humanos , Masculino , Úlceras Bucales/parasitología , Hueso Paladar/parasitología , Bolsa Periodontal/parasitologíaRESUMEN
Myiasis is the invasion of living tissue of humans and other mammals by the eggs or larvae of flies of the order of Diptera. It occurs mainly in the tropic, and is associated with inadequate public and personal hygiene. Oral myiasis in humans appears to be rare. This article records a case of oral myiasis caused by larvae of Hypoderma bovis. Two different pathologic soft tissue sockets were observed in the vestibular sulcus at the level of the both deciduous laterals along the deep upper lip tissues.
