Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 4.825
Filtrar
1.
Int J Mol Sci ; 25(9)2024 May 03.
Artículo en Inglés | MEDLINE | ID: mdl-38732218

RESUMEN

Boronate esters are a class of compounds containing a boron atom bonded to two oxygen atoms in an ester group, often being used as precursors in the synthesis of other materials. The characterization of the structure and properties of esters is usually carried out by UV-visible, infrared, and nuclear magnetic resonance (NMR) spectroscopic techniques. With the aim to better understand our experimental data, in this article, the density functional theory (DFT) is used to analyze the UV-visible and infrared spectra, as well as the isotropic shielding and chemical shifts of the hydrogen atoms 1H, carbon 13C and boron 11B in the compound 4-(4,4,5,5-tetramethyl-1,3,2-dioxoborolan-2-yl)benzaldehyde. Furthermore, this study considers the change in its electronic and spectroscopic properties of this particular ester, when its boron atom is coordinated with a fluoride anion. The calculations were carried out using the LSDA and B3LYP functionals in Gaussian-16, and PBE in CASTEP. The results show that the B3LYP functional gives the best approximation to the experimental data. The formation of a coordinated covalent B-F bond highlights the remarkable sensitivity of the NMR chemical shifts of carbon, oxygen, and boron atoms and their surroundings. Furthermore, this bond also highlights the changes in the electron transitions bands n → π* and π → π* during the absorption and emission of a photon in the UV-vis, and in the stretching bands of the C=C bonds, and bending of BO2 in the infrared spectrum. This study not only contributes to the understanding of the properties of boronate esters but also provides important information on the interactions and responses optoelectronic of the compound when is bonded to a fluorine atom.


Asunto(s)
Benzaldehídos , Benzaldehídos/química , Espectroscopía de Resonancia Magnética , Teoría Funcional de la Densidad , Flúor/química , Boro/química , Modelos Moleculares , Ésteres/química , Espectrofotometría Infrarroja , Estructura Molecular , Iones/química
2.
J Nanobiotechnology ; 22(1): 183, 2024 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-38622691

RESUMEN

BACKGROUND: The use of cells as carriers for the delivery of nanoparticles is a promising approach in anticancer therapy, mainly due to their natural properties, such as biocompatibility and non-immunogenicity. Cellular carriers prevent the rapid degradation of nanoparticles, improve their distribution, reduce cytotoxicity and ensure selective delivery to the tumor microenvironment. Therefore, we propose the use of phagocytic cells as boron carbide nanoparticle carriers for boron delivery to the tumor microenvironment in boron neutron capture therapy. RESULTS: Macrophages originating from cell lines and bone marrow showed a greater ability to interact with boron carbide (B4C) than dendritic cells, especially the preparation containing larger nanoparticles (B4C 2). Consequently, B4C 2 caused greater toxicity and induced the secretion of pro-inflammatory cytokines by these cells. However, migration assays demonstrated that macrophages loaded with B4C 1 migrated more efficiently than with B4C 2. Therefore, smaller nanoparticles (B4C 1) with lower toxicity but similar ability to activate macrophages proved to be more attractive. CONCLUSIONS: Macrophages could be promising cellular carriers for boron carbide nanoparticle delivery, especially B4C 1 to the tumor microenvironment and thus prospective use in boron neutron capture therapy.


Asunto(s)
Terapia por Captura de Neutrón de Boro , Nanopartículas , Boro , Línea Celular Tumoral , Nanopartículas/metabolismo , Macrófagos
3.
Sci Rep ; 14(1): 8265, 2024 04 09.
Artículo en Inglés | MEDLINE | ID: mdl-38594281

RESUMEN

Boron neutron capture therapy (BNCT) is a type of targeted particle radiation therapy with potential applications at the cellular level. Spinal cord gliomas (SCGs) present a substantial challenge owing to their poor prognosis and the lack of effective postoperative treatments. This study evaluated the efficacy of BNCT in a rat SCGs model employing the Basso, Beattie, and Bresnahan (BBB) scale to assess postoperative locomotor activity. We confirmed the presence of adequate in vitro boron concentrations in F98 rat glioma and 9L rat gliosarcoma cells exposed to boronophenylalanine (BPA) and in vivo tumor boron concentration 2.5 h after intravenous BPA administration. In vivo neutron irradiation significantly enhanced survival in the BNCT group when compared with that in the untreated group, with a minimal BBB scale reduction in all sham-operated groups. These findings highlight the potential of BNCT as a promising treatment option for SCGs.


Asunto(s)
Terapia por Captura de Neutrón de Boro , Neoplasias Encefálicas , Glioma , Neoplasias de la Médula Espinal , Ratas , Animales , Neoplasias Encefálicas/patología , Ratas Endogámicas F344 , Boro , Investigación Biomédica Traslacional , Compuestos de Boro/farmacología , Glioma/patología
4.
Inorg Chem ; 63(15): 6822-6835, 2024 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-38560761

RESUMEN

Boron-dipyrromethene (BODIPY) dyes are promising photosensitizers for cellular imaging and photodynamic therapy (PDT) owing to their excellent photophysical properties and the synthetically tunable core. Metalation provides a convenient way to overcome the drawbacks arising from their low aqueous solubility. New photo-/redox-responsive Co(III) prodrug chaperones are developed as anticancer PDT agents for efficient cellular delivery of red-light-active BODIPY dyes. The photobiological activity of heteroleptic Co(III) complexes derived from tris(2-pyridylmethyl)amine (TPA) and acetylacetone-conjugated PEGylated distyryl BODIPY (HL1) or its dibromo analogue (HL2), [CoIII(TPA)(L1/L2)](ClO4)2 (1 and 2), are investigated. The Co(III)/Co(II) redox potential is tuned using the Co(III)-TPA scaffold. Complex 1 displays the in vitro release of BODIPY on red light irradiation. Complex 2, having good singlet oxygen quantum yield (ΦΔ âˆ¼ 0.28 in DMSO), demonstrates submicromolar photocytotoxicity to HeLa cancer cells (IC50 ≈ 0.23 µM) while being less toxic to HPL1D normal cells in red light. Cellular imaging using the emissive complex 1 shows mitochondrial localization and significant penetration into the HeLa tumor spheroids. Complex 2 shows supercoiled DNA photocleavage activity and apoptotic cell death through phototriggered generation of reactive oxygen species. The Co(III)-BODIPY prodrug conjugates exemplify new type of phototherapeutic agents with better efficacy than the organic dyes alone in the phototherapeutic window.


Asunto(s)
Antineoplásicos , Fotoquimioterapia , Porfobilinógeno/análogos & derivados , Profármacos , Humanos , Boro/farmacología , Luz Roja , Colorantes , Profármacos/farmacología , Cobalto/farmacología , Fármacos Fotosensibilizantes/efectos de la radiación , Antineoplásicos/efectos de la radiación , Compuestos de Boro/farmacología , Compuestos de Boro/efectos de la radiación , Oxígeno Singlete/metabolismo , Luz
5.
Med Oncol ; 41(5): 104, 2024 Apr 04.
Artículo en Inglés | MEDLINE | ID: mdl-38573420

RESUMEN

It has been proposed that boron neutron capture therapy (BNCT) holds promise as a treatment modality for melanoma. However, the effectiveness of boron agents in delivery remains a critical issue to be addressed for BNCT. To this end, phenylboronic acid, which exhibits good water solubility and low cytotoxicity similar to BPA, has been investigated as a potential nuclear-targeting boron agent. The boron concentration of phenylboronic acid was found to be 74.47 ± 12.17 ng/106 B16F10 cells and 45.77 ± 5.64 ng/106 cells in the nuclei. Molecular docking experiments were conducted to investigate the binding of phenylboronic acid to importin proteins involved in nuclear transport. The potential of phenylboronic acid to serve as a desirable nucleus-delivery boron agent for neutron capture therapy in melanoma warrants further exploration.


Asunto(s)
Ácidos Borónicos , Melanoma , Terapia por Captura de Neutrón , Humanos , Boro , Simulación del Acoplamiento Molecular
6.
Bull Environ Contam Toxicol ; 112(5): 71, 2024 Apr 29.
Artículo en Inglés | MEDLINE | ID: mdl-38684523

RESUMEN

The remarkable optical properties and strong biocompatibility of carbon dots make them highly promising for applications in biochemical sensing and environmental testing. These carbon dots possess a surface that is easily modifiable. In this study, carbon dots have been successfully synthesized and modified by the addition of N and B dopants using the microwave method, along with the functionalization of their surface functional groups with bovine serum albumin (BSA). The maximum fluorescence intensity of N, B-CDs is observed at 462 nm when excited at a wavelength of 352 nm. N, B-CDs have a spherical size with a diameter ranging from 2 to 6 nm, confirmed by UV-Vis absorption spectra and the presence of functional groups in the FT-IR absorption patterns. BSA-functionalized N, B-CDs as the fluorescent probe demonstrate great potential as a sensor for Pb(II) ions in water, with a very low detection limit of 1.05 µg/L. This research could contribute to the development of fluorescence nanosensors.


Asunto(s)
Boro , Carbono , Plomo , Nitrógeno , Puntos Cuánticos , Plomo/análisis , Plomo/química , Boro/química , Carbono/química , Nitrógeno/química , Nitrógeno/análisis , Puntos Cuánticos/química , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/química , Albúmina Sérica Bovina/química , Monitoreo del Ambiente/métodos , Espectrometría de Fluorescencia , Colorantes Fluorescentes/química
7.
Ulus Travma Acil Cerrahi Derg ; 30(4): 236-241, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38634852

RESUMEN

BACKGROUND: The continuous advancement in medical and surgical techniques has led to a rise in the frequency of abdominal operations, subsequently increasing the incidence of intra-abdominal adhesions. Over 90% of laparotomies result in postoperative intra-abdominal adhesions. This study investigates the effect of a 5% boric acid solution on the development of intra-abdominal adhesions in rats, using an adhesion model. METHODS: This study was conducted with two groups: a control group, in which the adhesion model was applied without any treatment, and a boric acid group, which was treated with a 5% boric acid solution. Each group comprised 16 rats. On the 14th postoperative day, the rats were sacrificed, re-explored, and the developed adhesions were evaluated both macroscopically and microscopically. The data from macroscopic and microscopic scoring were analyzed using the Mann-Whitney U test in the IBM Statistical Package for the Social Sciences (SPSS) Statistics 24 program. A p-value of less than 0.05 was considered statistically significant. This research was supported by the Manisa Celal Bayar University Scientific Research Projects Commission. RESULTS: A statistically significant difference was observed between the boric acid-treated group and the control group, with the boric acid group showing a significant decrease in adhesion development both macroscopically and microscopically (p<0.05). CONCLUSION: In the future, boron could play a significant role in reducing and preventing intra-abdominal adhesions after surgery. This investigation could pave the way for further research into the mechanism by which boric acid prevents the development of intra-abdominal adhesions. Moreover, it is imperative to explore the potential side effects of intra-abdominal boron application at the optimum concentration of the solution.


Asunto(s)
Cavidad Abdominal , Boro , Humanos , Animales , Ratas , Laparotomía , Ácidos Bóricos , Adherencias Tisulares
8.
Physiol Plant ; 176(3): e14320, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38686642

RESUMEN

Many nucleoside triphosphate-diphosphohydrolases (NTPDases/APYRASEs, APYs) play a key role in modulating extracellular nucleotide levels. However, the Golgi-localized APYs, which help control glycosylation, have rarely been studied. Here, we identified AtAPY1, a gene encoding an NTPDase in the Golgi apparatus, which is required for cell wall integrity and plant growth under boron (B) limited availability. Loss of function in AtAPY1 hindered cell elongation and division in root tips while increasing the number of cortical cell layers, leading to swelling of the root tip and abundant root hairs under low B stress. Further, expression pattern analysis revealed that B deficiency significantly induced AtAPY1, especially in the root meristem and stele. Fluorescent-labeled AtAPY1-GFP localized to the Golgi stack. Biochemical analysis showed that AtAPY1 exhibited a preference of UDP and GDP hydrolysis activities. Consequently, the loss of function in AtAPY1 might disturb the homoeostasis of NMP-driven NDP-sugar transport, which was closely related to the synthesis of cell wall polysaccharides. Further, cell wall-composition analysis showed that pectin content increased and borate-dimerized RG-II decreased in apy1 mutants, along with a decrease in cellulose content. Eventually, altered polysaccharide characteristics presumably cause growth defects in apy1 mutants under B deficiency. Altogether, these data strongly support a novel role for AtAPY1 in mediating responses to low B availability by regulating cell wall integrity.


Asunto(s)
Apirasa , Proteínas de Arabidopsis , Arabidopsis , Boro , Pared Celular , Aparato de Golgi , Arabidopsis/genética , Arabidopsis/crecimiento & desarrollo , Arabidopsis/enzimología , Arabidopsis/metabolismo , Pared Celular/metabolismo , Boro/metabolismo , Boro/deficiencia , Aparato de Golgi/metabolismo , Proteínas de Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Apirasa/metabolismo , Apirasa/genética , Regulación de la Expresión Génica de las Plantas , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/genética , Raíces de Plantas/metabolismo , Meristema/genética , Meristema/crecimiento & desarrollo , Meristema/metabolismo , Pectinas/metabolismo
9.
Food Chem ; 449: 139264, 2024 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-38593724

RESUMEN

In this study, a microelectrode array sensor based on boron and nitrogen co-doped vertical graphene (BNVG) was assembled to quantify salicylic acid (SA) in living plants. The influence of B and N contents on the electrochemical reaction kinetics and SA response signal was investigated. A microneedle sensor with three optimized BNVG microelectrodes (3.57 at.% B and 3.27 at.% N) was used to quantitatively analyze SA in the 0.5-100 µM concentration range and pH 4.0-9.0, with limits of detection of 0.14-0.18 µM. Additionally, a quantitative electrochemical model database based on the BNVG microelectrode sensor was constructed to monitor the growth of cucumbers and cauliflowers, which confirmed that the SA level and plant growth rate were positively correlated. Moreover, the SA levels in various vegetables and fruits purchased from the market were measured to demonstrate the practical application prospects for on-site inspection and evaluation.


Asunto(s)
Boro , Técnicas Electroquímicas , Frutas , Grafito , Microelectrodos , Nitrógeno , Ácido Salicílico , Verduras , Grafito/química , Ácido Salicílico/análisis , Verduras/química , Frutas/química , Técnicas Electroquímicas/instrumentación , Boro/química , Nitrógeno/análisis , Agujas , Cucumis sativus/química , Técnicas Biosensibles/instrumentación , Límite de Detección
10.
Appl Radiat Isot ; 209: 111330, 2024 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-38657372

RESUMEN

Boron neutron capture therapy (BNCT) has received extensive attention as an advanced binary radiotherapy method. However, BNCT still faces poor selectivity of boron agent and is insufficient boron content in tumor tissues. To improve the tumor-targeted ability and boron content, this research aims to design, synthesize and preliminary evaluate a new borane agent Carborane-FAPI, which coupling the o-carborane to the compound skeleton of a mature fibroblast activating protein (FAP) inhibitor (FAPI). FAP is a tumor-associated antigen. FAP expressed lowly in normal organs and highly expressed in tumors, so it is a potential target for diagnosis and treatment. Boronophenylalanine (BPA) is the most widely investigated BNCT drug in present. Compared with BPA, the boron content of a single molecule is increased and drug targeting is enhanced. The results show that Carboaren-FAPI has low toxicity to normal cells, and selective enrichment in tumor tissues. It is a promising boron drug that has the potential to be used in BNCT.


Asunto(s)
Boranos , Terapia por Captura de Neutrón de Boro , Boro , Terapia por Captura de Neutrón de Boro/métodos , Humanos , Animales , Ratones , Proteínas de la Membrana/metabolismo , Endopeptidasas , Serina Endopeptidasas/metabolismo , Gelatinasas/metabolismo , Compuestos de Boro/uso terapéutico , Compuestos de Boro/farmacocinética , Línea Celular Tumoral
11.
Int J Biol Macromol ; 264(Pt 1): 130575, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38432270

RESUMEN

Hydroxypropyl guar gum (HPG) is a critical thickener to increase viscosity and lubrication to improve the water-based hydraulic fracturing efficiency. However, current crosslinkers require a large amount of HPG (>0.3 wt%) to form gel with sufficient viscosity, and high concentrations of HPG may cause adverse effects to the production and the environment. In this study, a novel starch microsphere silica­boron crosslinker (SMSB) was developed using starch microspheres as a carrier and γ-aminopropyl triethoxy silane (KH550) as a modifier with an in-house method. Both the rheology and surface reactions of the SMSB-HPG crosslinking system were studied using multiple laboratory experiments and molecular dynamics simulation. The results showed that SMSB crosslinker caused multi-site cross-linking with low concentration (only 0.2 wt%) of HPG molecules, reducing the twisting of single molecular chain in the crosslinking system, enhancing the cross-linking strength between molecular chains, and making HPG molecular chains stretcher in the aqueous solution. The apparent viscosity and viscoelasticity of the HPG system were substantially higher than the organoboron crosslinker, and the temperature resistance of the SMSB-HPG crosslinking system was up to 140 °C. This study provides an alternative green crosslinker for more sustainable industrial applications and provides theoretical basis for the modification of biomaterials.


Asunto(s)
Boro , Polisacáridos , Almidón , Silicio , Microesferas , Galactanos , Mananos , Gomas de Plantas , Agua
12.
Chem Commun (Camb) ; 60(26): 3523-3526, 2024 Mar 26.
Artículo en Inglés | MEDLINE | ID: mdl-38446177

RESUMEN

Boron nanosheets (BNSs) are reported as a new phosphatase mimicking nanozyme. Surprisingly, the catalytic rate of BNSs is up to 17 times those of known phosphatase mimicking nanozymes. By adding polyols and Lewis bases, the catalytic activity of BNSs was attributed to the Lewis acidity of the B centers of the BNSs. Theoretical investigation shows that the B centers are responsible for the catalytic hydrolysis of phosphoesters. Moreover, the biomimetic activity of the BNSs was further explored for enhancing anticancer therapy through nanozyme-catalyzed prodrug conversion.


Asunto(s)
Neoplasias , Monoéster Fosfórico Hidrolasas , Humanos , Boro , Hidrólisis , Neoplasias/tratamiento farmacológico , Catálisis
13.
PLoS One ; 19(3): e0298331, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38530838

RESUMEN

Electrochemical measurements, which exhibit high accuracy and sensitivity under low contamination, controlled electrolyte concentration, and pH conditions, have been used in determining various compounds. The electrochemical quantification capability decreases with an increase in the complexity of the measurement object. Therefore, solvent pretreatment and electrolyte addition are crucial in performing electrochemical measurements of specific compounds directly from beverages owing to the poor measurement quality caused by unspecified noise signals from foreign substances and unstable electrolyte concentrations. To prevent such signal disturbances from affecting quantitative analysis, spectral data of voltage-current values from electrochemical measurements must be used for principal component analysis (PCA). Moreover, this method enables highly accurate quantification even though numerical data alone are challenging to analyze. This study utilized boron-doped diamond (BDD) single-chip electrochemical detection to quantify caffeine content in commercial beverages without dilution. By applying PCA, we integrated electrochemical signals with known caffeine contents and subsequently utilized principal component regression to predict the caffeine content in unknown beverages. Consequently, we addressed existing research problems, such as the high quantification cost and the long measurement time required to obtain results after quantification. The average prediction accuracy was 93.8% compared to the actual content values. Electrochemical measurements are helpful in medical care and indirectly support our lives.


Asunto(s)
Cafeína , Café , Cafeína/análisis , Boro/química , Electrodos , Aprendizaje Automático , Electrólitos
14.
Plant Physiol Biochem ; 208: 108508, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38490152

RESUMEN

Boron (B) is essential for plant growth. However, the molecular mechanism of B transport in rapeseed (Brassica napus L.) is unknown well. Here, we report that B transporter BnaA4.BOR2 is involved in the transport of B from root to shoot and its distribution in shoot cell wall and flower in rapeseed. The results of GUS staining and in-situ PCR analysis showed that BnaA4.BOR2 is mainly expressed in cortex and endodermis of root tip meristem zone and endodermis of mature zone. BnaA4.BOR2 was mainly localized in plasma membrane and showed B transport activity in yeast. Overexpression of Bna4.BOR2 could rescue the phenotype of Arabidopsis mutant bor2-2 under low-B condition. Furthermore, knockout of BnaA4.BOR2 could significantly enhance the sensitivity of rapeseed mutants to B deficiency, including inhibition of root elongation and biomass decrease of roots and shoots. The B concentration in xylem sap of BnaA4.BOR2 mutants was significantly decreased under B deficiency, which resulted in significantly lower B concentrations in shoot cell wall at seedling stage and flower organ at reproductive stage compared to that of wild-type QY10. The growth of BnaA4.BOR2 mutants were severely inhibited, exhibiting a typical B-deficient phenotype of "flowering without seed setting", leading to a sharp decrease in seed yield in B deficient soil. Taken together, these results indicate that BnaA4.BOR2 is critical for rapeseed growth and seed yield production under low B level, which is mainly expressed in cortex and endodermis, and contributed to the transport of B from roots to shoots and its distribution in shoot.


Asunto(s)
Arabidopsis , Brassica napus , Brassica rapa , Brassica napus/metabolismo , Boro/metabolismo , Brassica rapa/metabolismo , Arabidopsis/metabolismo , Meristema/metabolismo , Raíces de Plantas/metabolismo
15.
Aquat Toxicol ; 269: 106886, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38458065

RESUMEN

Even though boron is a widely used element in various industries and a contributor to water pollution worldwide, few studies have examined the toxicity of boron in aquatic plants. EDTA is used to maintain aquatic plants cultures, however it is possible to modify the toxicity of metals. The objective of this study is to assess the toxicity of boron in aquatic plants and explore the impact of EDTA presence on the resulting toxic responses. Floating watermoss Salvinia natans and duckweed Lemna minor were exposed to concentrations ranging from 5 to 100 mg/L for 7 days and 1 to 60 mg/L for 3 days, respectively. Growth and photosynthetic activity parameters were investigated in the presence and absence of EDTA. Growth inhibitions in both aquatic plants were observed in a concentration-dependent manner, irrespective of the presence or absence of EDTA. For instance, based on the specific growth rate (leaves coverage), EC10 values for S. natans were calculated as 12.7 (9.9-15.3) mg/L and 8.0 (5.8-10.3) mg/L with and without EDTA, respectively. In the case of L. minor, EC10 values were calculated as 1.3 (0.8-1.89) mg/L and 2.0 (0.4-4.3) mg/L with EDTA without EDTA, respectively. Significant effects were also observed on the photosynthetic capacity, however there was no change in the increase of boron concentration. Generally, negligible effects of EDTA to the toxicity of boron were observed in the present study. By comparing toxicity results based on the presence and absence of EDTA, which is an essential element in the test medium, the results of this study are expected to be utilized for the ecological risk assessment of boron in aquatic ecosystems.


Asunto(s)
Araceae , Tracheophyta , Contaminantes Químicos del Agua , Boro/toxicidad , Ecosistema , Ácido Edético/farmacología , Contaminantes Químicos del Agua/toxicidad
16.
Biosens Bioelectron ; 253: 116162, 2024 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-38437748

RESUMEN

An electrochemiluminescence (ECL) biosensor with a pair of new ECL emitters and a novel sensing mechanism was designed for the high-sensitivity detection of microRNA-141 (miRNA-141). Sulfur-doped boron nitrogen quantum dots (S-BN QDs) were initially employed to modify the cathode of the bipolar electrode (BPE), while the anode reservoir was [Ir(dfppy)2(bpy)]PF6/TPrA system. The next step involved attaching H1-bound ultra-small WO3-x nanodots (WO3-x NDs) to the S-BN QDs-modified BPE cathode via DNA hybridization. A strong surface plasmon coupling (SPC) effect was observed between S-BN QDs and WO3-x NDs, which allowed for the enhancement of the red and visible ECL emission from S-BN QDs. After target-induced cyclic amplification to produce abundant Zn2+ and Au NPs-DNA3-Au NPs (Au NPs-S3-Au NPs), Zn2+ could cleave DNA at a nucleotide sequence-specific recognition site to release the WO3-x NDs, resulting in the first diminution of cathode ECL signal and the first enhancement of anode ECL signal. Moreover, the ECL signal at cathode decreased for the second time and the emission of [Ir(dfppy)2(bpy)]PF6 was continuously enhanced after the introduction of Au nanoparticles-S3-Au nanoparticles on the cathode surface. Our sensing mode with a dual "on-off" signal conversion strategy shows a good detection capability for miRNAs ranging from 10-17 to 10-10 M, with a limit of detection (LOD) as low as 10-17 M, which has great application potential in biomedical research and clinical diagnosis.


Asunto(s)
Técnicas Biosensibles , Nanopartículas del Metal , MicroARNs , Oro , Boro , Transferencia de Energía , Nitrógeno , Azufre , ADN
17.
J Mol Graph Model ; 129: 108763, 2024 06.
Artículo en Inglés | MEDLINE | ID: mdl-38555799

RESUMEN

The graphene-like monolayer of carbon, boron and nitrogen that maintains the native hexagonal atomic lattice (BCN), is a novel semiconductor with special thermal properties. Herein, with the aid of a non-equilibrium molecular dynamics approach (NEMD), we study phonon thermal rectification in a hybrid system of pure graphene and BCN (G-BCN) in various configurations under a series of positive and negative temperature gradients. We begin by investigating the relation of thermal rectification to sample's mean temperature, T, and the imposed temperature difference, ΔT, between the two heat baths at its ends. We then move to explore the effect of varying strain levels of our sample on thermal rectification, followed by Kapitza resistance calculations at the G-BCN interface, which shed light on the interface effects on thermal rectification. Our simulation results reveal a BCN-configuration-dependent behavior of thermal rectification. Finally, the underlying mechanism leading to a preferred direction for phonons is studied using phonon density of states (DOS) on both sides of the G-BCN interface.


Asunto(s)
Grafito , Simulación de Dinámica Molecular , Carbono , Boro , Calor
18.
Dent Mater J ; 43(2): 255-262, 2024 Mar 29.
Artículo en Inglés | MEDLINE | ID: mdl-38432951

RESUMEN

Surface pre-reacted glass-ionomer (S-PRG) filler is a bioactive glass filler capable of releasing various ions. A culture medium to which was added an S-PRG filler eluate rich in boron was reported to enhance alkaline phosphatase (ALP) activity in human dental pulp-derived stem cells (hDPSC). To clarify the role of boron eluted from S-PRG fillers, the modified S-PRG filler eluate with different boron concentrations was prepared by using an anion exchange material. Therefore, elemental mapping analysis of anion exchange material, adsorption ratio, hDPSCs proliferation and ALP activity were evaluated. For statistical analysis, Kruskal-Wallis test was used, with statistical significance determined at p<0.05. ALP activity enhancement was not observed in hDPSC cultured in the medium that contained the S-PRG filler eluate from which boron had been removed. The result suggested the possibility that an S-PRG filler eluate with controlled boron release could be useful for the development of novel dental materials.


Asunto(s)
Resinas Acrílicas , Boro , Pulpa Dental , Dióxido de Silicio , Humanos , Boro/farmacología , Cementos de Ionómero Vítreo , Aniones , Células Madre
19.
ACS Sens ; 9(3): 1611-1619, 2024 Mar 22.
Artículo en Inglés | MEDLINE | ID: mdl-38471116

RESUMEN

Drug detection in biological solutions is essential in studying the pharmacokinetics of the body. Electrochemical detection is an accurate and rapid method, but measuring multiple drugs that react at similar potentials is challenging. Herein, we developed an electrochemical sensor using a boron-doped diamond (BDD) electrode modified with a molecularly imprinted polymer (MIP) to provide specificity in drug sensing. The MIP is a polymer material designed to recognize and capture template molecules, enabling the selective detection of target molecules. In this study, we selected the anticancer drug doxorubicin (DOX) as the template molecule. In the electrochemical measurements using an unmodified BDD, the DOX reduction was observed at approximately -0.5 V (vs Ag/AgCl). Other drugs, i.e., mitomycin C or clonazepam (CZP), also underwent a reduction reaction at a similar potential to that of DOX, when using the unmodified BDD, which rendered the accurate quantification of DOX in a mixture challenging. Similar measurements conducted in PBS using the MIP-BDD only resulted in a DOX reduction current, with no reduction reaction observed in the presence of mitomycin C and CZP. These results suggest that the MIP, whose template molecule is DOX, inhibits the reduction of other drugs on the electrode surface. Selective DOX measurement using the MIP-BDD was also possible in human plasma, and the respective limits of detection of DOX in PBS and human plasma were 32.10 and 16.61 nM. The MIP-BDD was durable for use in six repeated measurements, and MIP-BDD may be applicable as an electrochemical sensor for application in therapeutic drug monitoring.


Asunto(s)
Técnicas Electroquímicas , Polímeros Impresos Molecularmente , Humanos , Técnicas Electroquímicas/métodos , Boro/química , Mitomicina , Límite de Detección , Electrodos , Doxorrubicina
20.
J Environ Manage ; 356: 120655, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38513589

RESUMEN

High boron (B) stress degrades the soil environment and reduces plant productivity. Sugar beet has a high B demand and potential for remediation of B-toxic soils. However, the mechanism regarding the response of sugar beet plants and rhizosphere soil microbiome to high B stress is not clear. In the potted soil experiment, we set different soil effective B environments (0.5, 5, 10, 30, 50, and 100 mg kg-1) to study the growth status of sugar beets under different B concentrations, as well as the characteristics of soil enzyme activity and microbial community changes. The results showed that sugar beet growth was optimal at 5 mg kg-1 of B. Exceeding this concentration the tolerance index decreased. The injury threshold EC20 was reached at an available B concentration of 35.8 mg kg-1. Under the treatment of 100 mg kg-1, the B accumulation of sugar beet reached 0.22 mg plant-1, and the tolerance index was still higher than 60%, which had not yet reached the lethal concentration of sugar beet. The abundance of Acidobacteriota, Chloroflexi and Patescibacteria increased, which was beneficial to the resistance of sugar beet to high B stress. In summary, under high B stress sugar beet had strong tolerance, enhanced capacity for B uptake and enrichment, and changes in soil microbial community structure. This study provides a theoretical basis for clarifying the mechanism of sugar beet resistance to high B stress and soil remediation.


Asunto(s)
Beta vulgaris , Suelo , Suelo/química , Beta vulgaris/metabolismo , Beta vulgaris/microbiología , Boro , Rizosfera , Verduras , Azúcares/metabolismo
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...