RESUMEN
In this study, an inulin-type fructan (PGPI-1-a) was isolated from the roots of Platycodon grandiflorum. PGPI-1-a was composed of (2 â 1)-linked ß-D-fructofuranose (Fruf) and a terminal α-d-glucopyranose (Glcp) with a molecular weight of 12.1 kDa. PM2.5 exposure has brought a great threat to human health in recent years. Therefore, this study explored the effect of PGPI-1-a on the intestinal microbial community structure of rats exposed to PM2.5 using the animal model of PM2.5 inhalation exposure. The results showed that PGPI-1-a could regulate the intestinal microbiota by partly restoring the perturbed levels of Peptoniphilaceae_[G-2] and Lachnospiraceae_[G-2] caused by PM2.5 exposure. In addition, the relative abundance of Butyrivibrio, a butyric acid-producing genera, significantly increased after PGPI-1-a intervention. These results indicated that PGPI-1-a could improve the imbalance of intestinal microbiota due to PM2.5 exposure to a certain extent.
Asunto(s)
Fructanos/farmacología , Microbioma Gastrointestinal/efectos de los fármacos , Inulina/farmacología , Material Particulado/efectos adversos , Platycodon/química , Animales , Butyrivibrio/efectos de los fármacos , Clostridiales/efectos de los fármacos , Fructanos/análisis , Humanos , Inulina/análisis , Masculino , Microbiota , Peso Molecular , Raíces de Plantas/química , RatasRESUMEN
Lipolysis and biohydrogenation in ruminal animals promote the accumulation of saturated fatty acids in their meat and milk. Antibodies were generated against key ruminal lipase contributors Anaerovibrio lipolyticus, Butyrivibrio fibrisolvens, Propionibacterium avidum and acnes. An anti-Pseudomonas lipase antibody was generated to determine if an antibody against a purified protein would be more effective. Each bacterium was cultured and assayed without or with increasing levels of each antibody. Butyrivibrio fibrisolvens H17C also participates in biohydrogenation and therefore the antibody was tested to determine if it could effectively reduce biohydrogenation. Butyrivibrio fibrisolvens was assayed without and with the anti-B. fibrisolvens antibody and linoleic or α-linolenic acid. All antibodies were effective at reducing lipolysis with the anti-Pseudomonas lipase averaging a 78% reduction. The anti-B. fibrisolvens showed a tendency for a reduction (P=0.0713) in biohydrogenation products of α-linolenic acid. Results demonstrate that lipolysis and biohydrogenation can be immunologically inhibited in vitro.
Asunto(s)
Anticuerpos Antibacterianos/química , Ácidos Grasos/química , Lipólisis/fisiología , Propionibacterium/efectos de los fármacos , Butyrivibrio/efectos de los fármacos , Hidrogenación , Ácido Linoleico/química , Lipasa/metabolismo , Ácido alfa-Linolénico/químicaRESUMEN
This study investigated the effects of ensiled mulberry leaves (EML) and sun-dried mulberry fruit pomace (SMFP) on the ruminal bacterial and archaeal community composition of finishing steers. Corn grain- and cotton meal-based concentrate was partially replaced with EML or SMFP. The diets had similar crude protein (CP), neutral detergent fiber (NDF), and metabolizable energy. Following the feeding trial, the steers were slaughtered and ruminal liquid samples were collected to study the ruminal microbiome. Extraction of DNA, amplification of the V4 region of the 16S rRNA gene, and Illumina MiSeq pyrosequencing were performed for each sample. Following sequence de-noising, chimera checking, and quality trimming, an average of 209,610 sequences were generated per sample. Quantitative real-time PCR was performed to examine the selected bacterial species in the rumen. Our results showed that the predominant phyla were Bacteroidetes (43.90%), Firmicutes (39.06%), Proteobacteria (4.31%), and Tenericutes (2.04%), and the predominant genera included Prevotella (13.82%), Ruminococcus (2.51%), Butyrivibrio (2.38%), and Succiniclasticum (2.26%). Compared to the control group, EML and SMFP groups had a higher abundance of total bacteria (p < 0.001); however, the bacterial community composition was similar among the three groups. At the phylum level, there were no significant differences in Firmicutes (p = 0.7932), Bacteroidetes (p = 0.2330), Tenericutes (p = 0.2811), or Proteobacteria (p = 0.0680) levels among the three groups; however, Fibrobacteres decreased in EML (p = 0.0431). At the genus level, there were no differences in Prevotella (p = 0.4280), Ruminococcus (p = 0.2639), Butyrivibrio (p = 0.4433), or Succiniclasticum (p = 0.0431) levels among the groups. Additionally, the dietary treatments had no significant effects on the archaeal community composition in the rumen. Therefore, EML and SMFP supplementation had no significant effects on the ruminal bacterial or archaeal community composition of finishing steers.
Asunto(s)
Archaea/efectos de los fármacos , Bacterias/efectos de los fármacos , Dieta/veterinaria , Microbioma Gastrointestinal/efectos de los fármacos , Morus/química , Extractos Vegetales/farmacología , Rumen/microbiología , Animales , Archaea/aislamiento & purificación , Bacterias/aislamiento & purificación , Bacteroidetes/efectos de los fármacos , Bacteroidetes/aislamiento & purificación , Butyrivibrio/efectos de los fármacos , Butyrivibrio/aislamiento & purificación , Bovinos , Firmicutes/efectos de los fármacos , Firmicutes/aislamiento & purificación , Frutas/química , Hojas de la Planta/química , Prevotella/efectos de los fármacos , Prevotella/aislamiento & purificación , Proteobacteria/efectos de los fármacos , Proteobacteria/aislamiento & purificación , Reacción en Cadena en Tiempo Real de la Polimerasa , Ruminococcus/efectos de los fármacos , Ruminococcus/aislamiento & purificación , Tenericutes/efectos de los fármacos , Tenericutes/aislamiento & purificaciónRESUMEN
BACKGROUND: Marine products can inhibit biohydrogenation in the rumen, but the mechanism is not clear. This study investigated a 20:5 n-3 rich supplement effects on rumen biohydrogenation, microbial change and fermentation characteristics in goats. RESULTS: The supplementation decreased 18:0 proportions in rumen fatty acids (P < 0.001), while it increased cis-9, trans-11 conjugated linoleic acid (CLA) (P < 0.001) and trans-10, cis-12 CLA proportions (P < 0.001). The supplement reduced the number of Butyrivibrio spp. and B. proteoclasticus (P < 0.01). Denaturing gradient gel electrophoresis redundancy analysis indicated that some species, mainly from the rumen of goats receiving the 2.5 and 5.0 g d(-1) supplement, were positively correlated with cis-9, trans-11 CLA proportions; some species, mainly from the rumen of control goats, were positively correlated with 18:0 proportions. The supplement reduced the NH3 -N concentrations and acetate molar proportions in the rumen (P < 0.05), but increased propionate and butyrate molar proportions (P < 0.01), and had no effect on total volatile fatty acid concentration. CONCLUSION: The supplement rich in 20:5 n-3 reduced the biohydrogenation of 18-carbon unsaturated fatty acids with a significant reduction of the 18:0 proportion and this was coupled with the suppression of the abundance of biohydrogenating bacteria and unknown bacteria.
Asunto(s)
Alimentación Animal/análisis , Butyrivibrio/efectos de los fármacos , Ácido Eicosapentaenoico/farmacología , Cabras , Rumen/microbiología , Rumen/fisiología , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Estudios Cruzados , ADN Bacteriano/genética , Dieta/veterinaria , Suplementos Dietéticos , Ácido Eicosapentaenoico/química , Ácidos Grasos , Fermentación , MasculinoRESUMEN
The effect of ethanol and methanol on growth of several ruminal bacterial strains was examined. Ethanol concentrations as low as 0.2% had a significant, but moderate, inhibitory effect on lag time or growth over time and 3.3% ethanol significantly inhibited maximum optical density obtained by both Selenomonas ruminantium and Butyrivibrio fibrisolvens. Little growth of either strain occurred at 10% ethanol concentrations. Methanol concentrations below 0.5% had little effect on either growth or maximum optical density of Selenomonas ruminantium whereas methanol concentrations below 3.3% had little effect on growth or maximum optical density of Butyrivibrio fibrisolvens. Higher methanol concentrations increasingly inhibited growth of both strains and no growth occurred at a 10% methanol concentration. Concentrations of ethanol or methanol used to add hydrophobic compounds to culture media should be kept below 1%.
Asunto(s)
Butyrivibrio/efectos de los fármacos , Etanol/farmacología , Metanol/farmacología , Rumen/microbiología , Selenomonas/efectos de los fármacos , Animales , Butyrivibrio/crecimiento & desarrollo , Medios de Cultivo , Relación Dosis-Respuesta a Droga , Selenomonas/crecimiento & desarrolloRESUMEN
BACKGROUND: Stoned olive pomace (SOP), which represents approximately 50% of the conversion process of olives to olive oil, is largely not utilised and creates costs for its disposal and has negative environmental impacts. In vitro trial experiments were employed to study the effect of feeds integrated with this bio-waste, which is rich in polyphenols, on rumen biohydrogenation, using sheep rumen liquor as inoculum. RESULTS: Fatty acid (FA) analysis and a polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) approach aimed at characterising the microbial community indicated that including SOP in feeds at the level of 50 g/kg and 90 g/kg induced changes in the FA profile and microbial populations. The simultaneous decrease of Butyrivibrio proteoclasticus and accumulation of vaccenic acid was observed. A depression in the populations of Neisseria weaveri, Ruminobacter amylophilus and other unclassified bacteria related to members of the Lachnospiraceae and Pasteurellaceae families was detected, suggesting that these microbial groups may be involved in rumen biohydrogenation. CONCLUSIONS: Supplementation of feeds with SOP alters the rumen bacterial community, including bacteria responsible for the hydrogenation of vaccenic acid to stearic acid, thereby modifying the FA profile of the rumen liquor. Hence, a use of SOP aimed to produce meat or dairy products enriched in functional lipids can be hypothesised.
Asunto(s)
Alimentación Animal , Suplementos Dietéticos , Ácidos Grasos Insaturados/metabolismo , Microbioma Gastrointestinal/efectos de los fármacos , Olea , Rumen/microbiología , Animales , Butyrivibrio/efectos de los fármacos , Butyrivibrio/genética , Electroforesis/veterinaria , Microbioma Gastrointestinal/genética , Hidrogenación/efectos de los fármacos , Técnicas In Vitro , Neisseria/efectos de los fármacos , Filogenia , Reacción en Cadena de la Polimerasa/veterinaria , Rumen/efectos de los fármacos , Rumen/metabolismo , OvinosRESUMEN
Previous studies have shown that adding fish oil (FO) to ruminant animal diets increased vaccenic acid (VA; t11 C18:1) accumulation in the rumen. Therefore, the objective of this study was to evaluate the effect of dietary FO amounts on selected strains of rumen bacteria involved in biohydrogenation. A single-flow continuous culture system consisting of four fermenters was used in a 4 × 4 Latin square design with four 9 days consecutive periods. Treatment diets were as follows: (i) control diet (53:47 forage to concentrate; CON), (ii) control plus FO at 0.5% (DM basis; FOL), (iii) control plus FO at 2% (DM basis; FOM) and (iv) control plus FO at 3.5% (DM basis; FOH). Fermenters were fed treatment diets three times daily at 120 g/day. Samples were collected from each fermenter on day 9 of each period at 1.5, 3 and 6 h post-morning feeding and then composited into one sample per fermenter. Increasing dietary FO amounts resulted in a linear decrease in acetate and isobutyrate concentrations and a linear decrease in acetate-to-propionate ratio. Propionate, butyrate, valerate and isovalerate concentrations were not affected by FO supplementation. Concentrations of C18:0 in fermenters linearly decreased, while concentrations of t10 C18:1 and VA linearly increased as dietary FO amounts increased. The concentrations of c9t11 and t10c12 conjugated linoleic acid were not affected by FO supplementation. The DNA abundance for Butyrivibrio fibrisolvens, Butyrivibrio vaccenic acid subgroup, Butyrivibrio stearic acid subgroup and Butyrivibrio proteoclasticus linearly decreased as dietary FO amounts increased. In conclusion, FO effects on trans fatty acid accumulation in the rumen may be explained in part by FO influence on Butyrivibrio group.
Asunto(s)
Butyrivibrio/efectos de los fármacos , Butyrivibrio/metabolismo , Aceites de Pescado/química , Ácidos Grasos trans/química , Ácidos Grasos trans/farmacología , Animales , Medios de Cultivo/química , Fermentación , Modelos Biológicos , Rumiantes , Ácidos Grasos trans/administración & dosificaciónRESUMEN
The hydrolysis of free fatty acids from lipids is a prerequisite for biohydrogenation, a process that effectively saturates free fatty acids. Anaerovibrio lipolyticus 5s and Butyrivibrio fibrisolvens have long been thought to be the major contributors to ruminal lipolysis; however, Propionibacterium avidum and acnes recently have been identified as contributing lipase activity in the rumen. In order to further characterize the lipase activity of these bacterial populations, each was grown with three different lipid substrates, olive oil, corn oil, and flaxseed oil (3 %). Because different finishing rations contain varying levels of glycogen (a source of free glucose) this study also documented the effects of glucose on lipolysis. P. avidum and A. lipolyticus 5s demonstrated the most rapid rates (P < 0.05) of lipolysis for cultures grown with olive oil and flaxseed oil, respectively. A. lipolyticus, B. fibrisolvens, and P. avidum more effectively hydrolyzed flaxseed oil than olive oil or corn oil, especially in the presence of 0.02 % glucose. Conversely, P. acnes hydrolyzed corn oil more readily than olive oil or flaxseed oil and glucose had no effect on lipolytic rate. Thus, these bacterial species demonstrated different specificities for oil substrates and different sensitivities to glucose.
Asunto(s)
Butyrivibrio/enzimología , Glucosa/metabolismo , Lipasa/metabolismo , Aceites de Plantas/metabolismo , Propionibacterium/enzimología , Rumen/microbiología , Veillonellaceae/enzimología , Animales , Butyrivibrio/efectos de los fármacos , Butyrivibrio/crecimiento & desarrollo , Bovinos , Aceite de Maíz/metabolismo , Medios de Cultivo , Glucosa/farmacología , Aceite de Linaza/metabolismo , Lipólisis/efectos de los fármacos , Aceite de Oliva , Propionibacterium/efectos de los fármacos , Propionibacterium/crecimiento & desarrollo , Rumen/efectos de los fármacos , Especificidad por Sustrato , Veillonellaceae/efectos de los fármacos , Veillonellaceae/crecimiento & desarrolloRESUMEN
Four lactating cows fitted with ruminal cannulae and fed a grass silage-based diet were used in a 4 × 4 Latin square with 28-d periods to investigate the effects of incremental dietary fish oil (FO) supplementation (0, 75, 150, or 300 g/d) on the flow of fatty acids at the omasum and populations of rumen bacteria capable of biohydrogenation. FO decreased silage intake and ruminal volatile fatty acid concentrations and promoted an increase in molar butyrate and propionate proportions at the expense of acetate. Extensive ruminal biohydrogenation of 20:5(n-3) and 22:6(n-3) resulted in corresponding increases in numerous 20- and 22-carbon unsaturated fatty acids at the omasum. Omasal flow of several 20-, 21-, and 22-carbon all-cis (n-3) PUFA exceeded the intake from FO. Supplements of FO also induced a dose-dependent decrease in 18:0 and increased trans 18:1 and trans 18:2 flow at the omasum. Trans-11 was the major 18:1 intermediate in digesta, while FO induced quadratic increases in trans-10 18:1 flow, reaching a maximum of 300 g/d. FO had no substantial influence on omasal flow of CLA. Results suggest that one or more fatty acids in FO inhibit the reduction of trans-18:1 and trans-18:2 intermediates by ruminal microorganisms. qPCR based on 16S rRNA genes in omasal digesta indicated that key Butyrivibrio spp. declined linearly in response to FO. Dose-dependent increases in ruminal outflow of biohydrogenation intermediates containing one or more trans double bonds in response to FO has major implications for host metabolism and the nutritional quality of ruminant foods.
Asunto(s)
Alimentación Animal/análisis , Butyrivibrio/efectos de los fármacos , Bovinos/microbiología , Dieta/veterinaria , Aceites de Pescado/farmacología , Lactancia/fisiología , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Butyrivibrio/clasificación , Bovinos/fisiología , Estudios Cruzados , Suplementos Dietéticos , Relación Dosis-Respuesta a Droga , Ácidos Grasos/metabolismo , Femenino , Aceites de Pescado/química , Omaso/metabolismo , Reacción en Cadena de la Polimerasa/veterinaria , Rumen/efectos de los fármacos , Rumen/microbiología , Sensibilidad y EspecificidadRESUMEN
BACKGROUND: Health-promoting polyunsaturated fatty acids (PUFA) are abundant in forages grazed by ruminants and in vegetable and fish oils used as dietary supplements, but only a small proportion of PUFA finds its way into meat and milk, because of biohydrogenation in the rumen. Butyrivibrio fibrisolvens plays a major role in this activity. The aim of this study was to investigate the mechanisms by which PUFA affect the growth of B. fibrisolvens, how PUFA are metabolized and the metabolic response to growth in the presence of PUFA. RESULTS: Linoleic acid (LA; cis-9, cis-12-18:2) and alpha-linolenic acid (LNA; cis-9, cis-12, cis-15-18:3) increased the lag phase of B. fibrisolvens JW11, LNA having the greater effect. Growth was initiated only when the PUFA had been converted to vaccenic acid (VA; trans-11-18:1). The major fish oil fatty acids, eicosapentaenoic acid (EPA; 20:5(n-3)) and docosahexaenoic acid (DHA; 22:6(n-3)), were not metabolized and prevented growth. Cellular integrity, as determined fluorimetrically by propidium iodide (PI) ingression, was affected as much by 18:1 fatty acids, including VA, as 18:2 fatty acids. The methyl esters of LNA, LA, EPA and DHA had no effect on growth or other measurements. The ATP pool decreased by 2/3 when LA was added to growing bacteria, whereas most acyl CoA pools decreased by >96%. CONCLUSIONS: It was concluded that biohydrogenation occurs to enable B. fibrisolvens to survive the bacteriostatic effects of PUFA, and that the toxicity of PUFA is probably mediated via a metabolic effect rather than disruption of membrane integrity.
Asunto(s)
Butyrivibrio/efectos de los fármacos , Ácidos Grasos Insaturados/farmacología , Acilcoenzima A/análisis , Adenosina Trifosfato/análisis , Animales , Butyrivibrio/metabolismo , Membrana Celular/efectos de los fármacos , Medios de Cultivo , Ácidos Grasos Insaturados/metabolismo , Citometría de Flujo , Hidrogenación , Ácido Linoleico/metabolismo , Ácido Linoleico/farmacología , Metabolismo de los Lípidos , Ovinos/microbiología , Lactato de Sodio/farmacologíaRESUMEN
tet(32) Was identified in three bacterial isolates and in metagenomic DNA from the human oral cavity. The regions immediately flanking the gene were found to have similarities to the mobile elements TnB1230 from Butyrivibrio fibrisolvens, ATE-3 from Arcanobacterium pyogenes, and CTn5 from Clostridium difficile.
Asunto(s)
Boca/microbiología , Proteínas Represoras/genética , Arcanobacterium/efectos de los fármacos , Arcanobacterium/genética , Butyrivibrio/efectos de los fármacos , Butyrivibrio/genética , Clostridioides difficile/efectos de los fármacos , Clostridioides difficile/genética , Elementos Transponibles de ADN/genética , Elementos Transponibles de ADN/fisiología , Modelos Genéticos , Datos de Secuencia Molecular , Filogenia , Proteínas Represoras/clasificaciónRESUMEN
Ruminal microorganisms hydrogenate polyunsaturated fatty acids (PUFA) present in forages and thereby restrict the availability of health-promoting PUFA in meat and milk. The aim of this study was to investigate PUFA metabolism and the influence of PUFA on members of the ruminal microflora. Eleven of 26 predominant species of ruminal bacteria metabolised linoleic acid (LA; cis-9,cis-12-18:2) substantially. The most common product was vaccenic acid (trans-11-18:1), produced by species related to Butyrivibrio fibrisolvens. alpha-Linolenic acid (LNA; cis-9,cis-12,cis-15-18:3) was metabolised mostly by the same species. The fish oil fatty acids, eicosapentaenoic acid (EPA; 20:5(n - 3)) and docosahexaenoic acid (DHA; 22:6(n - 3)) were not metabolised. Cellulolytic bacteria did not grow in the presence of any PUFA at 50 microg ml(-1), nor did some butyrate-producing bacteria, including the stearate producer Clostridium proteoclasticum, Butyrivibrio hungatei and Eubacterium ruminantium. Toxicity to growth was ranked EPA > DHA > LNA > LA. Cell integrity, as measured using propidium iodide, was damaged by LA in all 26 bacteria, but to different extents. Correlations between its effects on growth and apparent effects on cell integrity in different bacteria were low. Combined effects of LA and sodium lactate in E. ruminantium and C. proteoclasticum indicated that LA toxicity is linked to metabolism in butyrate-producing bacteria. PUFA also inhibited the growth of the cellulolytic ruminal fungi, with Neocallimastix frontalis producing small amounts of cis-9,trans-11-18:2 (CLA) from LA. Thus, while dietary PUFA might be useful in suppressing the numbers of biohydrogenating ruminal bacteria, particularly C. proteoclasticum, care should be taken to avoid unwanted effects in suppressing cellulolysis.
Asunto(s)
Butyrivibrio/metabolismo , Clostridium/metabolismo , Eubacterium/metabolismo , Ácidos Grasos Insaturados/metabolismo , Hongos/metabolismo , Ácido Linoleico/metabolismo , Rumen/microbiología , Animales , Butyrivibrio/efectos de los fármacos , Butyrivibrio/crecimiento & desarrollo , Clostridium/efectos de los fármacos , Clostridium/crecimiento & desarrollo , Eubacterium/efectos de los fármacos , Eubacterium/crecimiento & desarrollo , Ácidos Grasos Insaturados/toxicidad , Hongos/efectos de los fármacos , Hongos/crecimiento & desarrollo , Ácido Linoleico/toxicidad , Viabilidad Microbiana/efectos de los fármacosRESUMEN
tet(W) was found responsible for tetracycline resistance (MICs, 4 to > or =32 microg ml(-1)) in dominant bifidobacterial species from the gastrointestinal tracts of healthy humans. The gene from Bifidobacterium longum H66 proved to be identical over a 2.6-kbp region to the recently described tet(W) determinant of Butyrivibrio fibrisolvens.
Asunto(s)
Proteínas Bacterianas/genética , Bifidobacterium/efectos de los fármacos , Heces/microbiología , Resistencia a la Tetraciclina/genética , Antibacterianos/farmacología , Bifidobacterium/clasificación , Bifidobacterium/genética , Bifidobacterium/aislamiento & purificación , Butyrivibrio/efectos de los fármacos , Butyrivibrio/genética , Humanos , Pruebas de Sensibilidad Microbiana , Datos de Secuencia Molecular , Filogenia , Análisis de Secuencia de ADN , Tetraciclina/farmacologíaRESUMEN
This study demonstrates for the first time the in vitro transfer of the erythromycin resistance gene erm(B) between two obligate anaerobes, the human spore-forming pathogen Clostridium difficile and the rumen commensal Butyrivibrio fibrisolvens, suggesting that this event might occur also in the natural environment.
Asunto(s)
Proteínas Bacterianas/genética , Butyrivibrio/genética , Clostridioides difficile/genética , Resistencia a Medicamentos/genética , Transferencia de Gen Horizontal , Metiltransferasas/genética , Animales , Butyrivibrio/efectos de los fármacos , Bovinos , Clostridioides difficile/efectos de los fármacos , Datos de Secuencia Molecular , Rumen/microbiologíaRESUMEN
The proanthocyanidin polymer fractions of the leaves of the forage legume Dorycnium rectum were analysed by acid catalysis with benzyl mercaptan, NMR and ES-MS. The results showed that D. rectum differs from other temperate proanthocyanidin-containing forage legumes in that the range of polymers extends up to very high degrees of polymerisation. Three fractions were characterised as low, medium, and high molecular weight proanthocyanidin fractions with mean degree of polymerisations of 10.3, 41 and 127, respectively. Epigallocatechin was the most abundant extension unit and the terminating flavan-3-ols comprised largely catechin and gallocatechin units in equal proportions. Formation of thiolyated dimer products showed the interflavan-linkages of the lower molecular weight proanthocyanidins to be predominantly C4-->C8 with a small amount of C4-->C6. ES-MS spectra distinguished lower from higher polymeric proanthocyanidins from M2- to M8(2)-. The antibacterial activity of proanthocyanidin fractions against pure cultures of microbes selected from the ruminal population to represent fibre degrading, proteolytic and hyper ammonia producing bacteria in broth culture was evaluated. The activity of proanthocyanidin fractions against Clostridium aminophilum, Butyrivibrio fibrisolvens and Clostridium proteoclasticum was significantly dependent on their structure but not so against Ruminococcus albus and Peptostreptococcus anaerobius. The latter observation was unique in that they were sensitive to all proanthocyanidin fractions evaluated, even at the lowest concentration (100 microg/ml). The results suggest the effects of the extractable proanthocyanidins on rumen microbes should be considered when evaluating an alternative proanthocyanidin-containing forage source for ruminants, such as D. rectum.
Asunto(s)
Antibacterianos/farmacología , Fabaceae/química , Proantocianidinas/farmacología , Rumen/microbiología , Animales , Antibacterianos/química , Butyrivibrio/efectos de los fármacos , Clostridium/efectos de los fármacos , Fabaceae/metabolismo , Estructura Molecular , Peptostreptococcus/efectos de los fármacos , Proantocianidinas/química , Proantocianidinas/aislamiento & purificación , Ruminococcus/efectos de los fármacos , Especificidad de la Especie , Análisis Espectral , Relación Estructura-Actividad , Factores de TiempoRESUMEN
This research developed a community genome array (CGA) to assess the effects of Acacia angustissima on rumen microbiology. A. angustissima produces non-protein amino acids as well as tannins, which may be toxic to animals, and CGA was used to assess the effects of this plant on the ecology of the rumen. CGAs were developed using a 7.5 cmx2.5 cm nylon membrane format that included up to 96 bacterial genomes. It was possible to separately hybridize large numbers of membranes at once using this mini-membrane format. Pair-wise cross-hybridization experiments were conducted to determine the degree of cross-hybridization between strains; cross-hybridization occurred between strains of the same species, but little cross-reactivity was observed among different species. CGAs were successfully used to survey the microbial communities of animals consuming an A. angustissima containing diet but quantification was not precise. To properly quantify and validate the CGA, Fibrobacter and Ruminococcus populations were independently assessed using 16S rDNA probes to extracted rRNA. The CGA detected an increase in these populations as acacia increased in the diet, which was confirmed by rRNA analysis. There was a great deal of variation among strains of the same species in how they responded to A. angustissima. However, in general Selenomonas strains tended to be resistant to the tannins in the acacia while Butyrivibrio fibrisolvens was sensitive. On the other hand some species, like streptococci, varied. Streptococcus bovis-like strains were sensitive to an increase in acacia in the diet while Streptococcus gallolyticus-like strains were resistant. Strep. gallolyticus has independently been shown to be resistant to tannins. It is concluded that there is significant variation in tannin resistance between strains of the same species. This implies that there are specific molecular mechanisms at play that are independent of the phylogenetic position of the organism.