RESUMEN
The motivation of our study was to examine the acute effects of intravenously administered a single bolus dose of silver nanoparticles (AgNPs) on rat spermatogenesis and seminiferous tubules morphology. In the treated rats compared to the vehicle treated control animals, the experiments revealed a size-dependent (20nm and 200nm), dose-dependent (5 and 10mg/kg body mass) and time-dependent (24h, 7 and 28days) decrease the epididymal sperm count measured by histological methods. In parallel AgNPs injection increased the level of DNA damage in germ cells, as measured by alkaline comet assay. Histological examination of the testes showed change in the testes seminiferous tubule morphometry in 200nm Ag NPs treated rats. No change of body weight, adipose tissue distribution and the frequency of abnormal spermatozoa was observed. Twenty nanometers AgNP appeared to be more toxic than 200nm ones.
Asunto(s)
Epidídimo/efectos de los fármacos , Nanopartículas del Metal/toxicidad , Mutágenos/toxicidad , Compuestos de Plata/toxicidad , Espermatozoides/efectos de los fármacos , Animales , Peso Corporal/efectos de los fármacos , Ensayo Cometa , Daño del ADN , Relación Dosis-Respuesta a Droga , Epidídimo/patología , Inyecciones Intravenosas , Masculino , Nanopartículas del Metal/clasificación , Mutágenos/clasificación , Tamaño de la Partícula , Ratas , Ratas Wistar , Túbulos Seminíferos/efectos de los fármacos , Túbulos Seminíferos/patología , Compuestos de Plata/clasificación , Recuento de Espermatozoides/métodos , Espermatogénesis/efectos de los fármacos , Espermatogénesis/genética , Espermatozoides/patología , Factores de TiempoRESUMEN
OBJECTIVE: To evaluate the biocompatibility of nano-silver base inorganic antibacterial agents and compare the cytotoxicity in vitro among six types of nano-silver base inorganic antibacterial agents. METHODS: FUMAT T200-4, HN300, Novaron, Kangwang, MOD and SR1000 were diluted to different concentrations, such as 100 g/L, 50 g/L, 25 g/L and 12.5 g/L. The cytotoxicity in vitro of these agents on rat's fibroblast was assayed with MTT method. And the grades of cytotoxicity were compared. RESULTS: High concentrations of nano-silver base inorganic antibacterial agents had cytotoxic effects on rat's fibroblasts L-929. As the concentration decreased, the cytotoxicity decreased. No cytotoxic effects were observed at or below the concentration of 25 g/L. FUMAT T200-4, Kongwang and SR1000, with the carrier of phosphate zirconium, had less cytotoxity than the others. CONCLUSIONS: Nano-Silver base inorganic antibacterial agents, such as FUMAT T200-4, Kangwang, SR1000, have good biocompatibility. And they have the possibility of clinical application. The safe concentration of these agents is at or below 25 g/L.