Electrocution as an alternative euthanasia method to blunt force trauma to the head followed by exsanguination for non-viable piglets.

BACKGROUND: On farms, the currently approved and most widely practised method of euthanising non-viable piglets is blunt force trauma to the head followed by exsanguination. However, the use of this method is criticised due to public perceptions and aversion to the methodology by caretakers. Therefore, electrocution after electrical stunning was examined as an alternative approach in 80 hybrid piglets. Initially, electrocution was simulated with finite element analysis using a computer piglet-model, where current density in the heart was visualised and size and position of the electrodes were defined. The following step investigated electrical parameters for electrocution in anaesthetised piglets; first, with a constant voltage power source and then with a constant current power source. The electrical stunning was examined using the constant current supply. Finally, the results of electrical stunning and electrocution were verified in 25 healthy piglets with a body weight between 1 and 2 kg. Unconsciousness was proven by testing palpebral, corneal and nociceptive reflexes. Time of death was confirmed by electroencephalography (EEG) and electrocardiography (ECG) records. RESULTS: Stunning succeeded with the preset of 1.3 A and 50 Hz, placing the electrodes on both sides of the head between the eyes and ears using different timespans between 8 and 20 s. Prolonged electrical flow resulted in reduced paddling movements after the epileptic seizure, and allowed undisturbed reflex tests and installation of electrodes for EEG and ECG recording during electrocution. Using 0.75 A and 400 Hz, pin-shaped electrodes were first positioned on both sides of the chest for 5 s, followed by a break of 20-30 s and a second current flow, whereby the electrodes were placed above the withers and the sternum for 5 s. Cardiac arrest and an isoelectric EEG were induced within 3 min after the onset of the electrical flow through the chest. The most obvious indicator of effective stunning and electrocution was termination of rhythmic breathing. Piglets with cardiac arrest showed only single gasps lasting up to 3 min after electrocution. CONCLUSIONS: The evaluated stunning and electrocution protocol might ease concerns about timely piglet euthanasia. However, this should be verified in non-viable piglets to exclude influencing factors like dehydration and diseases.

The identification of effective welfare indicators for laboratory-housed macaques using a Delphi consultation process.

Despite the importance for both animal welfare and scientific integrity of effective welfare assessment in non-human primates, there has been little or no consensus as what should be assessed. A Delphi consultation process was undertaken to identify the animal- and environment-based measures of welfare for laboratory-housed macaques and to determine their relative importance in on-site welfare assessments. One-hundred fifteen potential indictors were identified through a comprehensive literature search, followed by a two-round iterative electronic survey process to collect expert opinion. Stable group response and consensus about the validity, reliability, and feasibility of the proposed indicators (67.5% agreement) was achieved by the completion of Round Two. A substantially higher proportion of environment-based measures (72%: n = 44/61) were considered as valid, reliable, and feasible compared to the animal-based measures (22%: n = 12/54). The indicators that ranked most highly for assessing welfare were the presence of self-harm behaviours and the provision of social enrichment. This study provides an empirical basis upon which these indicators can be validated and then integrated into assessment tools developed for macaques and emphasises the need to include both animal- and environment-based indicators for accurate welfare monitoring.

Systems for evaluation of welfare on dairy farms.

Animal welfare is an essential component of dairy production and several systems exist to evaluate the welfare of dairy cows. Here, we review and compare three well-known systems that operate at farm level from around the world (FARM, Welfare Quality®, and The Code of Welfare) and discuss their advantages and limitations. Despite having some commonalities, the programs evaluate different elements. We also briefly review an emerging system (Integrated Diagnostic Welfare System) that might address some of the shortcomings of the existing systems, especially the possibility of automating the evaluation of animal well-being and identifying any cause of poor welfare. None of the aforementioned systems has been fully validated for their ability to assess animal welfare using independent measurements. The future holds increased attention around the well-being of dairy cows and increased use of sensing technologies. There is an urgent need for dairy welfare evaluation systems that are scientifically validated, holistic, and that can take advantage of the use of sensing technologies to continuously monitor animal welfare.

Identifying obstacles preventing the uptake of tunnel handling methods for laboratory mice: An international thematic survey.

Handling of laboratory mice is essential for experiments and husbandry, but handling can increase anxiety in mice, compromising their welfare and potentially reducing replicability between studies. The use of non-aversive handling (e.g., tunnel handling or cupping), rather than the standard method of picking mice up by the tail, has been shown to enhance interaction with a handler, reduce anxiety-like behaviours, and increase exploration and performance in standard behavioural tests. Despite this, some labs continue to use tail handling for routine husbandry, and the extent to which non-aversive methods are being used is currently unknown. Here we conducted an international online survey targeting individuals that work with and/or conduct research using laboratory mice. The survey aimed to identify the handling methods currently being used, and to determine common obstacles that may be preventing the wider uptake of non-aversive handling. We also surveyed opinions concerning the current data in support of non-aversive handling for mouse welfare and scientific outcomes. 390 complete responses were received and analysed quantitatively and thematically. We found that 35% report using tail handling only, and 43% use a combination of tail and non-aversive methods. 18% of respondents reported exclusively using non-aversive methods. The vast majority of participants were convinced that non-aversive handling improves animal welfare and scientific outcomes. However, the survey indicated that researchers were significantly less likely to have heard of non-aversive handling and more likely to use tail handling compared with animal care staff. Thematic analysis revealed there were concerns regarding the time required for non-aversive methods compared with tail handling, and that there was a perceived incompatibility of tunnel handling with restraint, health checks and other routine procedures. Respondents also highlighted a need for additional research into the impact of handling method that is representative of experimental protocols and physiological indicators used in the biomedical fields. This survey highlights where targeted research, outreach, training and funding may have the greatest impact on increasing uptake of non-aversive handling methods for laboratory mice.

Keel bone fractures induce a depressive-like state in laying hens.

In commercial flocks of laying hens, keel bone fractures (KBFs) are prevalent and associated with behavioural indicators of pain. However, whether their impact is severe enough to induce a depressive-like state of chronic stress is unknown. As chronic stress downregulates adult hippocampal neurogenesis (AHN) in mammals and birds, we employ this measure as a neural biomarker of subjective welfare state. Radiographs obtained longitudinally from Lohmann Brown laying hens housed in a commercial multi-tier aviary were used to score the severity of naturally-occurring KBFs between the ages of 21-62 weeks. Individual birds' transitions between aviary zones were also recorded. Focal hens with severe KBFs at 3-4 weeks prior to sampling (n = 15) had lower densities of immature doublecortin-positive (DCX+) multipolar and bipolar neurons in the hippocampal formation than focal hens with minimal fractures (n = 9). KBF severity scores at this time also negatively predicted DCX+ cell numbers on an individual level, while hens that acquired fractures earlier in their lives had fewer DCX+ neurons in the caudal hippocampal formation. Activity levels 3-4 weeks prior to sampling were not associated with AHN. KBFs thus lead to a negative affective state lasting at least 3-4 weeks, and management steps to reduce their occurrence are likely to have significant welfare benefits.

[Ethics in Farm animals: The role of the veterinarian in animal protection on the example of bovines]. / Ethik in der Nutztierhaltung: Die Rolle der Tierärzte im Tierschutz am Beispiel Rind.

INTRODUCTION: In the present study, farm animal practitioners in Switzerland were questioned about their reported violations of the animal welfare law in cattle and their response to the detection of violations in the year 2017. The answers from 34 participants could be included in the evaluation. In 2017, 7.8% of the farms seen by participating practices were found to be in violation of the animal welfare law. It could be shown that the participating veterinarians reported only a small part (8.7%) of the detected offenses to the veterinary offices. In 91.7% of the cases, they responded to the violations and in only 8.3% of the cases they did not respond or they waited. Most often, they informed the livestock owners about the violation (66.1%) or advised them on the implementation of the animal welfare law (24.0%). The most common reason for the decision of reporting violations or not was the severity of the offense or animal suffering. There are many reasons why violations are not reported. The improvement of the situation after a reference, advice or threat of reporting to the veterinary office and the perception of the violation as minor are the most common ones. In addition, veterinarians see their role more in information and advice than in surveillance and repression.

INTRODUCTION: Dans le cadre de la présente étude, des vétérinaires praticiens dans le domaine des animaux de rente en Suisse ont été interrogés sur les violations de la Loi sur la protection des animaux qu'ils avaient constatées chez les bovins et sur leurs réactions à ces violations en 2017. Les réponses de 34 participants ont pu être incluses dans l'évaluation. En 2017, 7,8% des exploitations vues par les pratiques participantes ont été jugées en violation de la loi sur la protection des animaux. Il a pu être démontré que les vétérinaires participants n'ont signalé qu'une petite partie (8,7%) des infractions constatées aux services vétérinaires. Dans 91,7% des cas, ils ont réagi aux violations et dans seulement 8,3% des cas, ils ne l'ont pas fait ou ont attendu. Le plus souvent, ils ont rendu les éleveurs attentifs à la violation (66,1%) ou les ont conseillés sur la mise en œuvre de la loi sur la protection des animaux (24,0%). La raison la plus courante de la décision de signaler ou non les violations était la gravité de l'infraction ou l'estimation personnelle de la souffrance des animaux. Il existe de nombreuses raisons pour lesquelles les violations ne sont pas signalées. L'amélioration de la situation après une information, un avis ou une menace de dénonciation aux services vétérinaires et la perception de la violation comme mineure sont les plus courantes. De plus, les vétérinaires voient davantage leur rôle dans l'information et les conseils que dans la surveillance et la répression.

Antibiograms and risk factors of Salmonella isolates from laying hens and eggs in Jimma Town, South Western Ethiopia.

OBJECTIVES: Salmonella is the most important causes of foodborne illness especially from poultry and poultry products. So the aim of this study was to carryout phenotypic characterization, antimicrobials susceptibility pattern and risk factors of Salmonella isolates from farms and markets eggs, cloacae swabs of chickens and stool of egg collectors. A cross-sectional study was conducted from January 2018 to September 2018. Samples were, processed; Salmonella was isolated, phenotypically identified by OmniLog and antimicrobials susceptibility were carried out. RESULT: Over all; 11 (2.65%) of Salmonella enterica were phenotypically characterized out of 415 samples from farms egg content (n = 83), farms eggshell (n = 83), cloacae (n = 83), market eggshell (n = 83) and market egg contents (n = 83) with 2.4%, 0%, 2.4%, 4.8% and 3.6% prevalence, respectively. Out of isolates, 8 (72.72%) displayed multidrug resistance. All isolates showed susceptibility to Gentamicin, Kanamycin and Streptomycin. Lack of separating cracked eggs, washing hand, eggs stay longer unsold, and mixing excreta with feed were associated risk factors for Salmonella presence (P-value < 0.05). The presence of drug resistant Salmonella enterica within egg/and chicken can pose serious health problem. Good hygienic practices are important to reduce risk factors of Salmonella contamination.

Saving donkeys from the skin trade.

Georgina Mills explains how UK vets have been helping donkeys caught up in the skin trade in Brazil.

Discussing modern poultry farming systems-insights into citizen's lay theories.

A growing public concern, especially about animal welfare problems in poultry farming systems in the EU, has been addressed by numerous (mostly quantitative) studies. However, in-depth research about the underlying reasons for the lack of acceptance concerning modern poultry farming is rare. Debates on animal welfare are often polarized. Thus, this study involved 8 exploratory focus group discussions in 4 German cities. To understand the different positions and to simulate controversial debates on animal welfare, the study includes vegetarians/vegans (as a critical and involved group) and meat eaters. The actual level of knowledge about modern poultry production among participants was heterogeneous, varying between detailed specialized knowledge and misinformation. It was found that improvements for poultry animal welfare that have already been achieved, such as the ban of conventional cages for laying hens in the EU, were not yet recognized by the wider public. A central finding was that participants mostly use lay theories and conclude that the actual poultry husbandry systems make a high use of antibiotics necessary, which has a negative influence on the meat products and, thus, endangers consumer health. The results also indicate that an industrialized agri-food system (rather than farmers) is held primarily responsible for the perceived problems in the poultry value chain. Furthermore, participants are aware of their responsibility and also blame their buying behavior.

Assessing Youth Perceptions and Knowledge of Ethics at 4-H Horse Shows in Louisiana.

Due to the increased awareness of animal welfare issues and sensitized attitudes throughout society, youth animal projects provide a unique opportunity to assess and impact youth understanding and attitudes regarding animal welfare. Animal ethics training is not an uncommon requirement for participants in 4-H livestock programs. However, participants in 4-H horse projects are not universally held to the same standard. The purpose of this study was to assess youth knowledge, attitudes, and confidence in understanding of equine welfare at the 4-H state horse show, as well as to determine the beliefs and observations of unethical practices, and if differences exist between those parameters in youth competitors. The results suggest that 4-H competitors are interested in ethics and believe that unethical behavior rarely occurs within the state 4-H horse project. However, youth report observing common unethical behaviors at the show, suggesting a disconnect between what they believe is unethical and what they actually observe. Educational opportunities exist within these animal projects and should be enhanced to address the gap in understanding.

Whither Zoos? An Inescapable Question.

As a founding coeditor of the Journal of Applied Animal Welfare Science and a member of the International Advisory Committee of the Center for Zoo Animal Welfare and Ethics (CZAWE), I welcome this opportunity to publish for the third time (see earlier issues Volume 16, Number 4 [2013] and Volume 18, Supplement [2015]) selected proceedings from the Fourth Global Animal Welfare Congress of the Detroit Zoological Society's CZAWE. I have the honor of presenting a brief overview of the congress, entitled "Zoos and Aquariums as Welfare Centers: Ethical Dimensions and Global Commitment," which was held May 3, 2017, through May 6, 2017.

Are views towards egg farming associated with Brazilian and Chilean egg consumers' purchasing habits?

In many industrialised countries, public rejection of intensive animal production systems has led to the development of legislation and industry actions that have resulted in significant changes in animal care at the farm level. However, little is known about the views of citizens from emerging countries regarding animal production. The aims of this study were to explore the views of Brazilian and Chilean consumers towards egg farming, and to investigate if these views are associated with participants' eggs purchasing habits and reported willingness to pay (WTP) more for eggs produced in the conditions they perceive as important. In an open question, participants (n = 716) were asked to describe an ideal egg production farm and explain their reasons. This was followed by closed questions asking egg purchasing habits, willingness to pay for eggs produced in the conditions they perceive as important and demographic information. Participants main concerns were with animal welfare, naturalness, hygiene, production, and ethical aspects, which many associated with improved health, sensory, and nutritional quality of the eggs. The views of participants towards an ideal egg production farm were associated, to some extent, with type of egg purchasing habits and WTP a premium for organic or free-range eggs. Our results suggest a demand for more natural, animal friendly egg production systems; furthermore, they indicate a disconnect between lay citizens' expectations and industry practices, given that intensive confined systems typically fail to supply many of the expected characteristics.

Perception of animal welfare issues during Chinese transport and slaughter of livestock by a sample of stakeholders in the industry.

China is the world's biggest livestock producer, and has a rapidly expanding intensive livestock production in response to growing demand. The large size of the country and geographical dispersion of the livestock production systems means that animals are often transported long distances to slaughter. This study investigated perceptions of animal welfare issues by stakeholders in the Chinese transport and slaughter industry using utility scores and adaptive conjoint analysis. An initial workshop for experts in this field identified key concerns; these were then included in a questionnaire, which was distributed electronically to stakeholders. Stakeholders, particularly those with higher levels of education, were most concerned about the absence of pre-slaughter stunning and failure to maintain unconsciousness throughout the slaughter process. For all livestock species electrical stunning was considered the best method of stunning and blunt trauma the worst; for cattle and sheep stunning using a penetrating captive bolt was considered preferable to the use a percussive captive bolt. Other concerns considered very important were journey quality and livestock workers' experience and attitudes. Heat stress and closed-sided vehicles were of greater concern than cold stress. Loading facilities and journey length were considered of intermediate importance, while lairage and methods for catching chickens were of least concern. The importance of some welfare concerns, e.g. livestock having to remain standing during a journey, was more commonly recognised by stakeholders who reported a high level of knowledge and experience. Therefore, these welfare issues could be a focus for future training activities. Compared to respondents directly involved in livestock transport, respondents involved in teaching and researching within livestock production rated the presented animal welfare issues as more important. These results can be used to guide development of training programmes, animal welfare research, and certification and regulatory control to target challenges to animal welfare in livestock transport and slaughter in China.

Rabbit meat production and consumption: State of knowledge and future perspectives.

Since the 1970s, in some European countries rabbit meat production has progressively become a highly specialized industry, which has made Europe the second (after China) largest rabbit meat producer in the world. However, the industry is currently facing a critical period due to structural weaknesses, progressive and constant reductions in consumption, and raising criticism related to welfare conditions and other ethical issues. This trend is questioning the future of the rabbit meat industry, which could lead to the loss of knowledge and technical expertise acquired over decades of major investments and research efforts (a valuable cultural and professional heritage for future generations). In the present review, we provide an overview of the rabbit meat industry, market and value chain, we depict consumer's attitude towards rabbit meat, highlighting strengths and weaknesses, and factors driving their current purchase behavior. Finally, we will attempt to outline possible strategies to ensure a sustainable future for the production of rabbits for commercial meat purposes.

Antimicrobial resistance in aquaculture: Current knowledge and alternatives to tackle the problem.

Aquaculture is a rapidly growing industry that currently accounts for almost half of the fish used for human consumption worldwide. Intensive and semi-intensive practices are used to produce large stocks of fish, but frequent disease outbreaks occur, and the use of antimicrobials has become a customary practice to control them. The selective pressure exerted by these drugs, which are usually present at sub-therapeutic levels for prolonged periods in the water and the sediments, provides ideal conditions for the emergence and selection of resistant bacterial strains and stimulates horizontal gene transfer. It is now widely recognized that the passage of antimicrobial resistance genes and resistant bacteria from aquatic to terrestrial animal husbandry and to the human environment and vice versa can have detrimental effects on both human and animal health and on aquatic ecosystems. A global effort must be made to cease antimicrobial overuse in aquaculture and encourage stakeholders to adopt other disease-prevention measures. Shaping a new path is crucial to containing the increasing threat of antimicrobial resistance.