RESUMEN
The genus Daphnia O.F. Mller, 1776 (Crustacea: Cladocera) still has a confused taxonomy for several objective and subjective reasons. Still there are many taxa with inadequately described morphology, primarily among the subgenus Daphnia (Ctenodaphnia) Dybowski Grochowski, 1895. We provide a redescription of an Australian endemic taxon Daphnia (Ctenodaphnia) pusilla (Serventy, 1929) according to recent standards of morphological study with special attention to the thoracic limbs. We conclude that main differences between thoracic limbs of the subgenera D. (Ctenodaphnia) and Daphnia s. str. concern the limb I only as it is well-known among the cladocerans of other families. But still only a few species of D. (Ctenodaphnia) have been studied adequately, and efforts to redescribe their morphology need to be continued.
Asunto(s)
Cladóceros , Daphnia , Animales , Daphnia/anatomía & histología , HumanosRESUMEN
Predation is a major selective agent, so that many taxa evolved phenotypically plastic defensive mechanisms. Among them are many species of the microcrustacean genus Daphnia, which respond to an increased predation risk by developing inducible morphological alterations. Some of these features are obvious and easily recognized, e.g., crests in D. longicephala, while others are rather hidden, such as the bulkier shape of D. magna induced by the presence of the tadpole shrimp Triops. In this study we investigated the extraordinary diversity of morphological adaptations in the presence of predators with different foraging strategies in six predator-prey systems. For the first time we were able to analyze the unexposed and predator-exposed morphs comprehensively using three-dimensional scanning and reconstruction. We show that morphological changes are manifold in appearance between species and predators, and go beyond what has been known from previous 2D analyses. This further demonstrates the enormous trait flexibility of Daphnia. Interestingly, we found that among this variety some species share morphological strategies to counter a predator, while others use a different strategy against the same predator. Based on these intra- and interspecific comparisons, we discuss the mechanisms by which the respective defense might operate. These data therefore contribute to a deeper understanding of the inducible defenses' morphology as well as their diversified modes of operation in Daphnia, being a cornerstone for subsequent investigations, including the determination of costs associated with morphological change.
Asunto(s)
Daphnia/anatomía & histología , Conducta Predatoria/fisiología , Adaptación Fisiológica/fisiología , Animales , Cadena Alimentaria , Agua Dulce , Fenotipo , Alimentos MarinosRESUMEN
The expression of inducible morphological defenses in Daphnia in response to a single predator is a well-known phenomenon. However, predator-specific modifications of the same defensive traits as an adaption to different predator regimes is so far only described for Daphnia barbata. It is unknown if this accounts only for this species or if it is a more widespread, general adaptive response in the genus Daphnia. In the present study, we therefore investigated whether a clone of the pond-dwelling species Daphnia similis responds to different predatory invertebrates (Triops cancriformis; Notonecta maculata) with the expression of predator-specific modifications of the same defensive traits. We showed that Triops-exposed individuals express a significantly longer tail-spine, while body width decreased in comparison to control individuals. Additionally, they also expressed inconspicuous defenses, that is, significantly longer spinules on the dorsal ridge. The Notonecta-exposed D. similis showed a significantly longer tail-spine, longer spinules and a larger spinules bearing area on the dorsal ridge than control individuals as well. However, a geometric morphometric analysis of the head shape revealed significant, predator-specific changes. Triops-exposed individuals expressed a flattened head shape with a pronounced dorsal edge, while Notonecta-exposed individuals developed a high and strongly rounded head. Our study describes so far unrecognized inducible defenses of D. similis against two predators in temporary waters. Furthermore, the predator-dependent change in head shape is in concordance with the 'concept of modality', which highlights the qualitative aspect of natural selection caused by predators.
Asunto(s)
Cladóceros/anatomía & histología , Daphnia/anatomía & histología , Agua Dulce , Conducta Predatoria/fisiología , Animales , Cabeza/anatomía & histología , Fenotipo , Análisis de Componente PrincipalRESUMEN
BACKGROUND: Daphnia species reproduce by cyclic parthenogenesis involving both sexual and asexual reproduction. The sex of the offspring is environmentally determined and mediated via endocrine signalling by the mother. Interestingly, male and female Daphnia can be genetically identical, yet display large differences in behaviour, morphology, lifespan and metabolic activity. Our goal was to integrate multiple omics datasets, including gene expression, splicing, histone modification and DNA methylation data generated from genetically identical female and male Daphnia pulex under controlled laboratory settings with the aim of achieving a better understanding of the underlying epigenetic factors that may contribute to the phenotypic differences observed between the two genders. RESULTS: In this study we demonstrate that gene expression level is positively correlated with increased DNA methylation, and histone H3 trimethylation at lysine 4 (H3K4me3) at predicted promoter regions. Conversely, elevated histone H3 trimethylation at lysine 27 (H3K27me3), distributed across the entire transcript length, is negatively correlated with gene expression level. Interestingly, male Daphnia are dominated with epigenetic modifications that globally promote elevated gene expression, while female Daphnia are dominated with epigenetic modifications that reduce gene expression globally. For examples, CpG methylation (positively correlated with gene expression level) is significantly higher in almost all differentially methylated sites in male compared to female Daphnia. Furthermore, H3K4me3 modifications are higher in male compared to female Daphnia in more than 3/4 of the differentially regulated promoters. On the other hand, H3K27me3 is higher in female compared to male Daphnia in more than 5/6 of differentially modified sites. However, both sexes demonstrate roughly equal number of genes that are up-regulated in one gender compared to the other sex. Since, gene expression analyses typically assume that most genes are expressed at equal level among samples and different conditions, and thus cannot detect global changes affecting most genes. CONCLUSIONS: The epigenetic differences between male and female in Daphnia pulex are vast and dominated by changes that promote elevated gene expression in male Daphnia. Furthermore, the differences observed in both gene expression changes and epigenetic modifications between the genders relate to pathways that are physiologically relevant to the observed phenotypic differences.
Asunto(s)
Metilación de ADN , Daphnia/genética , Epigénesis Genética , Epigenómica/métodos , Regiones Promotoras Genéticas/genética , Animales , Daphnia/anatomía & histología , Daphnia/metabolismo , Femenino , Expresión Génica , Histonas/genética , Histonas/metabolismo , Lisina/metabolismo , Masculino , Metilación , Fenotipo , Factores SexualesRESUMEN
BACKGROUND: Several genetic lineages of obligate parthenogenetic Daphnia pulex, a common zooplankton species, have invaded Japan from North America. Among these, a lineage named JPN1 is thought to have started colonization as a single genotype several hundred to thousand years ago and subsequently produced many genotypes in Japan. To examine the phenotypic variations due to ecological drivers diverging the genotypes in new habitats, we measured heritability and variation in 17 traits, including life history, morphology and digestive traits, and the genetic distance among the D. pulex JPN1 genotypes in Japan. RESULTS: We found that most of the traits measured varied significantly among the genotypes and that heritability was highest in the morphological traits, followed by the digestive and life history traits. In addition, 93% of the variation in these traits was explained by the first three components in the principal component analysis, implying that variation of these heritable traits is not random but rather converged into a few directions. These relations among traits revealed the potential importance of predation pressures and food conditions as factors for diverging and selecting different genotypes. However, the magnitude of the difference in any single trait group did not correlate with the genetic distance. CONCLUSIONS: Our findings show that the divergent traits evolved within D. pulex JPN1 lineage without genetic recombination, since their ancestral clone invaded Japan. Large variations and covariations of the phenotypic traits, irrespective of the genetic distance among the genotypes, support the view that the invasive success of D. pulex JPN1 was promoted by a genetic architecture that allowed for large phenotypic variations with a limited number of functionally important mutations without recombination.
Asunto(s)
Daphnia/genética , Rasgos de la Historia de Vida , Animales , Daphnia/anatomía & histología , Daphnia/crecimiento & desarrollo , Genotipo , Japón , América del Norte , Partenogénesis , Fenotipo , ZooplanctonRESUMEN
The water flea Daphnia pulex, a small crustacean that lives in freshwater ponds, undergoes parthenogenesis and gamogenesis according to environmental conditions. In D. pulex, different morphological characteristics can be observed using a microscope during aging. In this study, we recorded the growth, reproduction, and survival indicators of parthenogenetic D. pulex females to analyze the morphological changes that occur during senescence. We observed the growth and development of the parthenogenetic females on the 7th, 14th, 21st, and 28th days by using scanning electron microscopy and ultrastructure of the gonads on the 7th, 14th and 21st days with transmission electron microscopy. We found that body length and reproductive capacity in the parthenogenetic individuals first increased and then decreased with aging, and the survival rate decreased gradually. The depression of the brood pouch on the back of the parthenogenetic females implied a decline in gonadal function and gradual aging. During senescence, the number of lipid droplets in the gonads of the parthenogenetic females decreased, and the integrity of the mitochondria and peroxisome was destroyed. The results of our study revealed the morphological characteristics of senescence in D. pulex.
Asunto(s)
Envejecimiento , Daphnia/anatomía & histología , Gónadas/anatomía & histología , Gónadas/ultraestructura , Partenogénesis , Animales , Daphnia/crecimiento & desarrollo , Femenino , Microscopía Electrónica de RastreoRESUMEN
Metabolic rate (MR) often scales with body mass (BM) following a power function of the form MR=aBM b , where log(a) is the allometric intercept and b is the allometric exponent (i.e. slope on a log-log scale). The variational properties of b have been debated, but very few studies have tested for genetic variance in b, and none have tested for a genotype-by-environment (G×E) interaction in b Consequently, the short-term evolutionary potentials of both b and its phenotypic plasticity remain unknown. Using 10 clones of a population of Daphnia magna, we estimated the genetic variance in b and assessed whether a G×E interaction affected b We measured MR on juveniles of different sizes reared and measured at three temperatures (17, 22 and 28°C). Overall, b decreased with increasing temperature. We found no evidence of genetic variance in b at any temperature, and thus no G×E interaction in b However, we found a significant G×E interaction in size-specific MR. Using simulations, we show how this G×E interaction can generate genetic variation in the ontogenetic allometric slope of animals experiencing directional changes in temperature during growth. This suggests that b can evolve despite having limited genetic variation at constant temperatures.
Asunto(s)
Metabolismo Basal/fisiología , Daphnia/genética , Daphnia/metabolismo , Temperatura , Adaptación Fisiológica , Animales , Tamaño Corporal , Daphnia/anatomía & histología , Daphnia/crecimiento & desarrollo , Genotipo , Consumo de OxígenoRESUMEN
Variation in eye size is ubiquitous across taxa. Increased eye size is correlated with improved vision and increased fitness via shifts in behavior. Tests of the drivers of eye size evolution have focused on macroevolutionary studies evaluating the importance of light availability. Predator-induced mortality has recently been identified as a potential driver of eye size variation. Here, we tested the influence of increased predation by the fish predator, the alewife (Alosa pseudoharengus) on eye size evolution in waterfleas (Daphnia ambigua) from lakes in Connecticut. We quantified the relative eye size of Daphnia from lakes with and without alewife using wild-caught and third-generation laboratory reared specimens. This includes comparisons between lakes where alewife are present seasonally (anadromous) or permanently (landlocked). Wild-caught specimens did not differ in eye size across all lakes. However, third-generation lab reared Daphnia from lakes with alewife, irrespective of the form of alewife predation, exhibited significantly larger eyes than Daphnia from lakes without alewife. This genetically based increase in eye size may enhance the ability of Daphnia to detect predators. Alternatively, such shifts in eye size may be an indirect response to Daphnia aggregating at the bottom of lakes. To test these mechanisms, we collected Daphnia as a function of depth and found that eye size differed in Daphnia found at the surface versus the bottom of the water column between anadromous alewife and no alewife lakes. However, we found no evidence of Daphnia aggregating at the bottom of lakes. Such results indicate that the evolution of a larger eye may be explained by a connection between eyes and enhanced survival. We discuss the cause of the lack of concordance in eye size variation between our phenotypic and genetic specimens and the ultimate drivers of eye size.
Asunto(s)
Daphnia/anatomía & histología , Peces , Conducta Predatoria , Distribución Animal , Animales , Connecticut , Ojo/anatomía & histología , Femenino , Cadena Alimentaria , LagosRESUMEN
We examined the morphology of Daphnia individuals maintained in our laboratory for several years, originally collected in Lake Inbanuma, Chiba, Japan. We determined partial sequences of the mitochondrial cytochrome c oxidase subunit I and 12S rRNA genes from specimens in the cultured material. These animals are morphologically similar to D. obtusa Kurz, 1874 , but genetically distinct from this species. Our detailed observation shows that the morphological characteristics in the female and male individuals of our material are highly congruent with those of D. mitsukuri Ishikawa, 1896 , which has not been identified positively for more than 120 years since its original description, with its taxonomic identity having been questioned for almost 90 years. Based on our morphological and genetic data, we conclude that D. mitsukuri should be regarded as a taxonomically valid species. A search among public DNA sequence databases suggests D. mitsukuri is also distributed in China, although these Chinese sequences have been labeled as 'Daphnia pulex', representing misidentification.
Asunto(s)
Daphnia/anatomía & histología , Daphnia/genética , Animales , Daphnia/clasificación , Femenino , Filogenia , Especificidad de la EspecieRESUMEN
The freshwater crustacean Daphnia is known for its ability to develop inducible morphological defences that thwart predators. These defences are developed only in the presence of predators and are realized as morphological shape alterations e.g. 'neckteeth' in D. pulex and 'crests' in D. longicephala. Both are discussed to hamper capture, handling or consumption by interfering with the predator's prey capture devices. Additionally, D. pulex and some other daphniids were found to armour-up and develop structural alterations resulting in increased carapace stiffness. We used scanning transmission electron microscopy (STEM) and confocal laser scanning microscopy (CLSM) to identify predator-induced structural and shape alterations. We found species specific structural changes accompanying the known shape alterations. The cuticle becomes highly laminated (i.e. an increased number of layers) in both species during predator exposure. Using nano- and micro-indentation as well as finite element analysis (FEA) we determined both: the structure's and shape's contribution to the carapace's mechanical resistance. From our results we conclude that only structural alterations are responsible for increased carapace stiffness, whereas shape alterations appear to pose handling difficulties during prey capture. Therefore, these defences act independently at different stages during predation.
Asunto(s)
Exoesqueleto/fisiología , Fenómenos Biomecánicos , Daphnia/fisiología , Exoesqueleto/anatomía & histología , Animales , Daphnia/anatomía & histología , Agua Dulce , Microscopía Confocal , Microscopía Electrónica de Rastreo , Microscopía Electrónica de TransmisiónRESUMEN
This study analyses the histological and cellular morphology of the testis and sperm development in the male Daphnia magna Straus 1820. Due to the rarity of males and predominately parthenogenetic lifecycle of Daphnia, there has been limited detailed information on males in contrast to the well-studied female. Using light and electron microscopy approaches, we describe the morphology of the testis during the progression from an immature to mature testis. The testis has an encasing muscular mesh sheath outside the basal lamina, beneath which is a thin somatic epithelial cell layer. Internal to the epithelium are the spermatogonial stem cells and subsequent syncytial clusters of the germ cells as they progress through spermatogenesis; spermatozoa occupy the entire testis in sexually mature D. magna. We describe the structure of developing and mature spermatozoa; mature spermatozoa are non-flagellated, ovoid in shape with plasmalemma filapodia and are encased in an extracellular capsule.
Asunto(s)
Daphnia/anatomía & histología , Daphnia/citología , Genitales Masculinos/anatomía & histología , Espermatogénesis , Espermatozoides/citología , Animales , Daphnia/ultraestructura , Genitales Masculinos/ultraestructura , Masculino , Espermatozoides/ultraestructura , Testículo/anatomía & histología , Testículo/ultraestructuraRESUMEN
Worldwide, urbanization leads to tremendous anthropogenic environmental alterations, causing strong selection pressures on populations of animals and plants. Although a key feature of urban areas is their higher temperature ("urban heat islands"), adaptive thermal evolution in organisms inhabiting urban areas has rarely been studied. We tested for evolution of a higher heat tolerance (CTMAX ) in urban populations of the water flea Daphnia magna, a keystone grazer in freshwater ecosystems, by carrying out a common garden experiment at two temperatures (20°C and 24°C) with genotypes of 13 natural populations ordered along a well-defined urbanization gradient. We also assessed body size and haemoglobin concentration to identify underlying physiological drivers of responses in CTMAX . We found a higher CTMAX in animals isolated from urban compared to rural habitats and in animals reared at higher temperatures. We also observed substantial genetic variation in thermal tolerance within populations. Overall, smaller animals were more heat tolerant. While urban animals mature at smaller size, the effect of urbanization on thermal tolerance is only in part caused by reductions in body size. Although urban Daphnia contained higher concentrations of haemoglobin, this did not contribute to their higher CTMAX . Our results provide evidence of adaptive thermal evolution to urbanization in the water flea Daphnia. In addition, our results show both evolutionary potential and adaptive plasticity in rural as well as urban Daphnia populations, facilitating responses to warming. Given the important ecological role of Daphnia in ponds and lakes, these adaptive responses likely impact food web dynamics, top-down control of algae, water quality, and the socio-economic value of urban ponds.
Asunto(s)
Aclimatación , Adaptación Fisiológica/genética , Tamaño Corporal , Daphnia/anatomía & histología , Daphnia/genética , Calor , Animales , Daphnia/fisiología , Ecosistema , Variación Genética , Genotipo , Lagos , UrbanizaciónRESUMEN
Altering thermal environments impose strong selection pressures on organisms, whose local persistence depends on adaptive phenotypic plastic and genetic responses. Thus far, adaptive change is monitored using phenotypic shifts or molecular markers, although inevitable obstacles are inherent in both methods. In order to circumvent these, it is necessary to find a causal link between adaptive alleles and fitness. Combining both approaches by linking genetic analyses and life-history measurements, a potential genotype-phenotype relationship can be assessed and adaptation at the molecular level demonstrated. For our study, clonal lineages of the freshwater keystone species D. galeata from seven different populations distributed along a latitudinal gradient across Europe were tested for local thermal adaptation in common garden experiments. Fitness-related life-history responses were quantified under different thermal regimes and experimental clones were genotyped at three candidate gene marker loci to investigate a potential genotype-phenotype association. The analyses of the life-history data showed a significant temperature effect on several fitness-related life-history traits recorded in our experiments. However, we could not detect evidence for a direct association at neither candidate gene locus between genotypes and life-history traits. The observed phenotypic shifts might therefore not be based on the tested marker loci EA, M and TF, or in general not coding sequence-based and thus rather reveal phenotypic plasticity in response to thermal variation. Nonetheless, we revealed significant genotype by environment (GxE) interactions at all tested loci, potentially reflecting a contribution of marker loci to certain life-history trait values and contribution of multiple genetic loci to phenotypic traits.
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Daphnia/genética , Termotolerancia/fisiología , Animales , Daphnia/anatomía & histología , Femenino , Estudios de Asociación Genética , Sitios Genéticos/genética , Reproducción/fisiología , Análisis de Secuencia de ADN , Temperatura , Termotolerancia/genéticaRESUMEN
Species of the genus Daphnia O.F. Müller, 1785 (Cladocera: Daphniidae) have become very important models in evolutionary biology research. Previous morphological and genetic evidence suggests that numerous closely related "species groups" exist within the subgenus Daphnia (Ctenodaphnia) Dybowski & Grochowski, 1895, containing both described and undescribed species. The Daphnia similis group is among these species groups. The aim of the present paper is to revise the taxonomy of the Daphnia (Ctenodaphnia) similis group in the Old World with both morphological and genetic evidence (based on mitochondrial COI and 12S rRNA genes). We found that there are at least four species in the Old World D. similis species group: D. similis Claus, 1876; D. sinensis Gu, Xu, Li, Dumont et Han, 2013; D. similoides Hudec, 1991 and D. inopinata sp. nov. These four taxa of the similis-group, confused previously with D. similis, have different distributional ranges in the Old World, from extremely wide, spanning several biogegraphic regions (as D. sinensis), to regional endemics (D. similoides) and even species known so far from a single locality (D. inopinata sp. nov.). The Daphnia similis group provides another example in the cladocerans whereby the study of males yields more valuable characters for taxonomy than the study of parthenogenetic females.
Asunto(s)
Daphnia/clasificación , Distribución Animal , Estructuras Animales/anatomía & histología , Estructuras Animales/crecimiento & desarrollo , Animales , Evolución Biológica , Tamaño Corporal , Daphnia/anatomía & histología , Daphnia/genética , Daphnia/crecimiento & desarrollo , Ecosistema , Femenino , Masculino , Tamaño de los Órganos , FilogeniaRESUMEN
Daphnia (Crustacea, Cladocera) are well known for their ability to form morphological adaptations to defend against predators. In addition to spines and helmets, the carapace itself is a protective structure encapsulating the main body, but not the head. It is formed by a double layer of the integument interconnected by small pillars and hemolymphatic space in between. A second function of the carapace is respiration, which is performed through its proximal integument. The interconnecting pillars were previously described as providing higher mechanical stability against compressive forces. Following this hypothesis, we analyzed the carapace structure of D. pulex using histochemistry in combination with light and electron microscopy. We found the distal integument of the carapace to be significantly thicker than the proximal. The pillars appear fibrous with slim waists and broad, sometimes branched bases where they meet the integument layers. The fibrous structure and the slim-waisted shape of the pillars indicate a high capacity for withstanding tensile rather than compressive forces. In conclusion they are more ligaments than pillars. Therefore, we measured the hemolymphatic gauge pressure in D. longicephala and indeed found the hemocoel to have a pressure above ambient. Our results offer a new mechanistic explanation of the high rigidity of the daphniid carapace, which is probably the result of a light-weight construction consisting of two integuments bound together by ligaments and inflated by a hydrostatic hyper-pressure in the hemocoel. J. Morphol. 277:1320-1328, 2016. © 2016 Wiley Periodicals, Inc.
Asunto(s)
Exoesqueleto/anatomía & histología , Daphnia/anatomía & histología , Resistencia a la Tracción , Exoesqueleto/fisiología , Exoesqueleto/ultraestructura , Animales , Fuerza Compresiva , Daphnia/fisiologíaRESUMEN
Predation is one of the most important drivers of natural selection. In consequence a huge variety of anti-predator defenses have evolved in prey species. Under unpredictable and temporally variable predation pressure, the evolution of phenotypically plastic defensive traits is favored. These "inducible defenses", range from changes in behavior, life history, physiology to morphology and can be found in almost all taxa from bacteria to vertebrates. An important group of model organisms in ecological, evolutionary and environmental research, water fleas of the genus Daphnia (Crustacea: Cladocera), are well known for their ability to respond to predators with an enormous variety of inducible morphological defenses. Here we report on the "twist", a body torsion, as a so far unrecognized inducible morphological defense in Daphnia, expressed by Daphnia barbata exposed to the predatory tadpole shrimp Triops cancriformis. This defense is realized by a twisted carapace with the helmet and the tail spine deviating from the body axis into opposing directions, resulting in a complete abolishment of bilateral symmetry. The twisted morphotype should considerably interfere with the feeding apparatus of the predator, contributing to the effectiveness of the array of defensive traits in D. barbata. As such this study does not only describe a completely novel inducible defense in the genus Daphnia but also presents the first report of a free living Bilateria to flexibly respond to predation risk by abandoning bilateral symmetry.
Asunto(s)
Cladóceros/fisiología , Daphnia/anatomía & histología , Daphnia/fisiología , Conducta Predatoria , Animales , Daphnia/ultraestructura , Torsión MecánicaRESUMEN
We reveal the neuroanatomy of the optic ganglia and central brain in the water flea Daphnia magna by use of classical neuroanatomical techniques such as semi-thin sectioning and neuronal backfilling, as well as immunohistochemical markers for synapsins, various neuropeptides and the neurotransmitter histamine. We provide structural details of distinct neuropiles, tracts and commissures, many of which were previously undescribed. We analyse morphological details of most neuron types, which allow for unravelling the connectivities between various substructural parts of the optic ganglia and the central brain and of ascending and descending connections with the ventral nerve cord. We identify 5 allatostatin-A-like, 13 FMRFamide-like and 5 tachykinin-like neuropeptidergic neuron types and 6 histamine-immunoreactive neuron types. In addition, novel aspects of several known pigment-dispersing hormone-immunoreactive neurons are re-examined. We analyse primary and putative secondary olfactory pathways and neuronal elements of the water flea central complex, which displays both insect- and decapod crustacean-like features, such as the protocerebral bridge, central body and lateral accessory lobes. Phylogenetic aspects based upon structural comparisons are discussed as well as functional implications envisaging more specific future analyses of ecotoxicological and endocrine disrupting environmental chemicals.
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Encéfalo/anatomía & histología , Daphnia/anatomía & histología , Ganglios de Invertebrados/anatomía & histología , Animales , Encéfalo/citología , Agregación Celular , Daphnia/citología , FMRFamida/metabolismo , Ganglios de Invertebrados/citología , Histamina/metabolismo , Imagenología Tridimensional , Modelos Biológicos , Neuronas/metabolismo , Neuropéptidos/metabolismo , Neurópilo/metabolismo , Corteza Olfatoria/anatomía & histología , Péptidos/metabolismo , Taquicininas/metabolismo , Vías Visuales/anatomía & histologíaRESUMEN
Phenotypic plasticity is the ability of a genotype to produce more than one phenotype in order to match the environment. Recent theory proposes that the major axis of genetic variation in a phenotypically plastic population can align with the direction of selection. Therefore, theory predicts that plasticity directly aids adaptation by increasing genetic variation in the direction favoured by selection and reflected in plasticity. We evaluated this theory in the freshwater crustacean Daphnia pulex, facing predation risk from two contrasting size-selective predators. We estimated plasticity in several life-history traits, the G matrix of these traits, the selection gradients on reproduction and survival, and the predicted responses to selection. Using these data, we tested whether the genetic lines of least resistance and the predicted response to selection aligned with plasticity. We found predator environment-specific G matrices, but shared genetic architecture across environments resulted in more constraint in the G matrix than in the plasticity of the traits, sometimes preventing alignment of the two. However, as the importance of survival selection increased, the difference between environments in their predicted response to selection increased and resulted in closer alignment between the plasticity and the predicted selection response. Therefore, plasticity may indeed aid adaptation to new environments.
Asunto(s)
Daphnia/anatomía & histología , Daphnia/genética , Variación Genética , Fenotipo , Selección Genética , Adaptación Biológica , Animales , Cadena AlimentariaRESUMEN
The synthetic flame retardant tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) has been frequently detected in natural waters, and its maximum concentration ever reported is 377 ng/L. However, information on the adverse effects of environmentally relevant concentrations of TDCIPP on aquatic organisms are totally unknown. In this study, <12-h old water fleas, D. magna, were exposed to concentrations of 0, 65±7.1, 550±33, or 6500±1400 ng/L TDCIPP, and dose- and time-dependent effects on reproduction and development were evaluated. Sequences of genes of D. magna were obtained from the National Center for Biotechnology Information and were used to develop PCR arrays for D. magna. Arrays were then used to study transcriptional responses of D. magna to TDCIPP. Exposure to environmentally relevant concentrations of TDCIPP significantly decreased fecundity as well as length of F0 and F1 generations. Transcriptional responses showed that, of the 155 genes tested, expressions of 57 genes were significantly changed, and some changes occurred following exposure to environmentally relevant concentrations (i.e., 65±7.1 and 550±23 ng/L). Furthermore, pathways related to protein synthesis and metabolism and endocytosis were considered to be significantly affected in a dose- and time-dependent manner and might be responsible for TDCIPP-induced reproductive and developmental toxicities.
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Daphnia/crecimiento & desarrollo , Daphnia/genética , Compuestos Organofosforados/toxicidad , Transcripción Genética/efectos de los fármacos , Contaminantes Químicos del Agua/toxicidad , Animales , Daphnia/anatomía & histología , Daphnia/efectos de los fármacos , Endocitosis/efectos de los fármacos , Exposición a Riesgos Ambientales/análisis , Regulación de la Expresión Génica/efectos de los fármacos , Reacción en Cadena de la Polimerasa , Reproducción/efectos de los fármacos , Soluciones , Factores de TiempoRESUMEN
Several studies of eye morphology have analysed macroevolutionary patterns in the diversity of eyes, and although these studies are often linked to environment or behaviour, they provide only indirect evidence of selection. Specific data to show the microevolutionary potential for adaptation by natural selection in eye morphology have been lacking. We document directional selection on eye size, an important determinant of visual capabilities, in a wild population of the freshwater microcrustacean Daphnia. We show that even slight changes in eye size may have major consequences for fitness. An increase in eye diameter of 19.9 µm - slightly more than one standard deviation - is associated with an increase in clutch size of one egg, or an increase of nearly 20% of the mean clutch size. Furthermore, relative eye size is genetically variable and thus could evolve in response to the observed selective pressure. We conclude that selection on incremental variation in eye size may have led to differences observed on broader taxonomic scales.