RESUMEN
Pure gonadal dysgenesis is a situation when the karyotype is 46, XY, but for various reasons there is a disorder of differentiation of Wolffian and Mullerian structures and in consequence the phenotype is female. It is known that abdominal gonads and the presence of Y chromosome allow to qualify this condition as a high risk of tumor. In most cases breast development is limited because of lack or low level of estrogen. A 27-year-old patient with differences of sexual development (DSD), was admitted to the Department of Endocrinological Gynecology for a control examination. In the history: dysgerminoma, primary amenorrhea and ambiguous karyotype. The patient has not taken hormonal replacement therapy. The breast development is Tanner stage V.
Asunto(s)
Disgerminoma , Disgenesia Gonadal 46 XY , Disgenesia Gonadal , Neoplasias Ováricas , Disgerminoma/diagnóstico , Femenino , Disgenesia Gonadal/diagnóstico , Disgenesia Gonadal/patología , Disgenesia Gonadal 46 XY/diagnóstico , Disgenesia Gonadal 46 XY/genética , Disgenesia Gonadal 46 XY/patología , Gónadas/patología , Humanos , Neoplasias Ováricas/patologíaRESUMEN
Reproductive health of men has declined over time including reduced semen quality specifically sperm count, increased incidence of infertility, and testicular cancers. Our recent findings suggest that these disease states possibly arise as a result of disruption of testicular stem cells biology by perinatal insults including exposure to endocrine disrupting chemicals. Testicular stem cells include relatively quiescent, very small embryonic-like stem cells (VSELs), and actively dividing spermatogonial stem cells (SSCs). Both VSELs and SSCs express estrogen receptors and are directly vulnerable to endocrine disruption. Exposing mice pups to estradiol (20 µg/pup/day on days 5-7) or diethylstilbestrol (2 µg/pup/day on days 1-5) affected spermatogenesis during adult life with reduced numbers of tubules in stage VIII, tetraploid cells and sperm. These mice were infertile and majority of diethylstilbestrol treated mice revealed testicular cancer-like changes. An increase in VSEL numbers, observed by both flow cytometry and qRT-PCR, was associated with marked reduction of c-KIT positive spermatogonial cells. VSELs undergo epigenetic changes due to endocrine disruption that results in blocked differentiation (impaired spermatogenesis) leading to reduced sperm count and infertility, and their excessive self-renewal initiates cancer-like changes in adult life. Thus, testicular dysgenesis syndrome (TDS) has a stem cell rather than a genetic basis.
Asunto(s)
Disruptores Endocrinos/efectos adversos , Exposición a Riesgos Ambientales/efectos adversos , Disgenesia Gonadal/inducido químicamente , Infertilidad Masculina/etiología , Salud del Hombre , Salud Reproductiva , Células Madre/efectos de los fármacos , Testículo/efectos de los fármacos , Factores de Edad , Animales , Diferenciación Celular/efectos de los fármacos , Disgenesia Gonadal/diagnóstico , Disgenesia Gonadal/metabolismo , Disgenesia Gonadal/fisiopatología , Humanos , Infertilidad Masculina/diagnóstico , Infertilidad Masculina/metabolismo , Infertilidad Masculina/fisiopatología , Masculino , Factores de Riesgo , Espermatogénesis/efectos de los fármacos , Células Madre/metabolismo , Células Madre/patología , Síndrome , Testículo/metabolismo , Testículo/patologíaRESUMEN
Ectopic splenic tissue in any location could represent accessory spleen, a common congenital abnormality, splenosis, the presence of numerous nodules of ectopic splenic tissue after spleen trauma/surgery or splenogonadal fusion, a rare congenital malformation found mostly in males. Ectopic splenic tissue in the ovary is usually found in the context of an extensive pelvic splenosis. Solitary ovarian splenosis presenting not as a part of pelvic splenosis, but rather as a solitary mass masquerading an ovarian tumor is extremely rare. Differential diagnosis in this case includes mostly splenosis and splenogonadal fusion of the discontinuous type. Relevant literature and possible pathogenesis are discussed.
Asunto(s)
Coristoma/patología , Enfermedades del Ovario/patología , Bazo , Coristoma/diagnóstico , Femenino , Disgenesia Gonadal/diagnóstico , Humanos , Persona de Mediana Edad , Enfermedades del Ovario/diagnóstico , Esplenosis/diagnósticoRESUMEN
Disorders of sex development (DSD) are defined as discrepancy between chromosomal, gonadal and anatomic sex. The basic principles for the management of DSD include a multidisciplinary approach for gender assignment. Clinical assessment includes a detailed history and examination of external genitalia. Most of the disorders with symmetrical gonades indicate hormonal cause while asymmetrical gonades are found in chromosomal DSDs. Karyotyping will indicate a 46XX DSD, 46 XY DSD or mosicism. Internal anatomy is defined by ultrasonography, genitoscopy and laparoscopy. Human chorionic gonadotrophins (hCG) stimulation test is carried out in under-virilised males to see the function of Leydig cells in testes. The Most common cause of 46XX DSD is congenital adrenal hyperplasia (CAH). The decision of gender assignment surgery is to be taken in a multidisciplinary environment and with informed consent of the parents. Most of 46 XX CAH patients, even if markedly virilised, and 46 XY complete androgen insensitivity syndrome are raised as females. Similarly, most of 5-α reductase deficiency and 17-ß hydroxysteroid dehydrogenase deficiency patients are assigned to the male gender. The decision in cases of mixed gondal dysgenesis and ovotesticular DSD is based on the development of external and internal genitalia. Patients with androgen biosynthetic defects, partial androgen insensitivity syndrome are usually assigned to the male gender.
Asunto(s)
Trastornos del Desarrollo Sexual/diagnóstico , 17-Hidroxiesteroide Deshidrogenasas/deficiencia , Hiperplasia Suprarrenal Congénita/diagnóstico , Síndrome de Resistencia Androgénica/diagnóstico , Colestenona 5 alfa-Reductasa/deficiencia , Trastorno del Desarrollo Sexual 46,XY/diagnóstico , Femenino , Disgenesia Gonadal/diagnóstico , Ginecomastia/diagnóstico , Humanos , Cariotipificación , Masculino , Errores Congénitos del Metabolismo Esteroideo/diagnósticoRESUMEN
Pfaffia glomerata (Spreng.) Pedersen, popularly known as "Brazilian ginseng," is used as medicinal plant in Brazil to treat inflammatory diseases in general. Previous studies showed that its extract increases the nitric oxide (NO) levels. Knowing that NO downregulates steroidogenesis and that alterations in the action/production of androgens during perinatal life could alter testis development, the present studies sought to investigate the reproductive toxicity of Pfaffia glomerata on male mice exposed to hydroalcoholic extract in utero and during lactation. The present study shows that P. glomerata extract does not alter body weight, tubular diameter and testis function in male mice. Although a reduction in the testis weight was observed in the animals that received the highest dose directly in early post-natal life, our findings show clearly that P. glomerata may not act as an endocrine disruptor, and it is not an "antiandrogenic" compound that could lead to testicular dysgenesis syndrome.
Asunto(s)
Disgenesia Gonadal/diagnóstico , Panax/química , Extractos Vegetales/toxicidad , Efectos Tardíos de la Exposición Prenatal/diagnóstico , Testículo/efectos de los fármacos , Andrógenos/biosíntesis , Animales , Animales Lactantes , Peso Corporal/efectos de los fármacos , Brasil , Modelos Animales de Enfermedad , Femenino , Disgenesia Gonadal/etiología , Disgenesia Gonadal/patología , Humanos , Lactancia , Masculino , Exposición Materna/efectos adversos , Ratones , Óxido Nítrico/metabolismo , Tamaño de los Órganos/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/administración & dosificación , Raíces de Plantas/química , Embarazo , Efectos Tardíos de la Exposición Prenatal/etiología , Efectos Tardíos de la Exposición Prenatal/patología , Testículo/patologíaRESUMEN
The differential diagnosis of differences or disorders of sex development (DSD) belongs to the most complex fields in medicine. It requires a multidisciplinary team conducting a synoptic and complementary approach consisting of thorough clinical, hormonal and genetic workups. This position paper of EU COST (European Cooperation in Science and Technology) Action BM1303 'DSDnet' was written by leading experts in the field and focuses on current best practice in genetic diagnosis in DSD patients. Ascertainment of the karyotpye defines one of the three major diagnostic DSD subclasses and is therefore the mandatory initial step. Subsequently, further analyses comprise molecular studies of monogenic DSD causes or analysis of copy number variations (CNV) or both. Panels of candidate genes provide rapid and reliable results. Whole exome and genome sequencing (WES and WGS) represent valuable methodological developments that are currently in the transition from basic science to clinical routine service in the field of DSD. However, in addition to covering known DSD candidate genes, WES and WGS help to identify novel genetic causes for DSD. Diagnostic interpretation must be performed with utmost caution and needs careful scientific validation in each DSD case.
Asunto(s)
Trastornos del Desarrollo Sexual/diagnóstico , Secuenciación del Exoma , Cariotipo , Secuenciación Completa del Genoma , Hiperplasia Suprarrenal Congénita/diagnóstico , Hiperplasia Suprarrenal Congénita/genética , Variaciones en el Número de Copia de ADN , Trastornos del Desarrollo Sexual/genética , Unión Europea , Disgenesia Gonadal/diagnóstico , Disgenesia Gonadal/genética , Humanos , Biología Molecular , Técnicas de Diagnóstico Molecular , Guías de Práctica Clínica como Asunto , Análisis de Secuencia de ADNRESUMEN
Patients with complete XY gonadal dysgenesis (GD) show a high predisposition to germ cell tumors (GCT). Patients with coexistence of GCT and GD have been reported previously. Here we present a 15-year-old girl with mixed GCT and GD who also developed an intra-abdominal synovial sarcoma one year after the treatment. This is the first report, to our knowledge, of synovial sarcoma associated with XY GD.
Asunto(s)
Neoplasias Abdominales/diagnóstico , Disgenesia Gonadal/diagnóstico , Neoplasias de Células Germinales y Embrionarias/diagnóstico , Neoplasias Ováricas/diagnóstico , Sarcoma Sinovial/diagnóstico , Neoplasias Abdominales/tratamiento farmacológico , Neoplasias Abdominales/cirugía , Adolescente , Niño , Resultado Fatal , Femenino , Humanos , Metástasis Linfática , Neoplasias de Células Germinales y Embrionarias/tratamiento farmacológico , Neoplasias de Células Germinales y Embrionarias/cirugía , Neoplasias Ováricas/tratamiento farmacológico , Neoplasias Ováricas/cirugía , Sarcoma Sinovial/tratamiento farmacológico , Sarcoma Sinovial/cirugíaRESUMEN
PURPOSE: In this report, we characterize the timing and behavior of malignant ovarian germ cell tumors (GCTs) in pediatric patients with dysgenetic gonads compared to those with normal gonadal development. PATIENTS AND METHODS: Patients from the Children's Oncology Group AGCT0132 with malignant ovarian GCTs were included. Within this population, we sought to identify patients with gonadoblastoma, streak ovaries, or other evidence of gonadal dysgenesis (GD). Patients with malignant GCTs containing one or more of the following histologies-yolk sac tumor, embryonal carcinoma, or choriocarcinoma-were included. Patients were compared with respect to event-free survival (EFS) and overall survival (OS). RESULTS: Nine patients with GD, including seven with gonadoblastoma (mean age, 9.3 years), were compared to 100 non-GD patients (mean age, 12.1 years). The estimated 3-year EFS for patients with GD was 66.7% (95% CI 28.2-87.8%) and for non-GD patients was 88.8% (95% CI 80.2-93.8%). The estimated 3-year OS for patients with GD was 87.5% (95% CI 38.7-98.1%) and for non-GD patients was 97.6% (95% CI of 90.6-99.4%). CONCLUSION: Patients presenting with nongerminomatous malignant ovarian GCTs in the context of GD have a higher rate of events and death than counterparts with normal gonads. These findings emphasize the importance of noting a contralateral streak ovary or gonadoblastoma at histology for any ovarian GCT and support the recommendation for early bilateral gonadectomy in patients known to have GD with Y chromosome material. In contrast to those with pure dysgerminoma, these patients may represent a high-risk group that requires a more aggressive chemotherapy regimen.
Asunto(s)
Disgenesia Gonadal/mortalidad , Neoplasias de Células Germinales y Embrionarias/mortalidad , Neoplasias Ováricas/mortalidad , Adolescente , Adulto , Niño , Preescolar , Supervivencia sin Enfermedad , Femenino , Disgenesia Gonadal/diagnóstico , Disgenesia Gonadal/patología , Disgenesia Gonadal/terapia , Humanos , Lactante , Recién Nacido , Neoplasias de Células Germinales y Embrionarias/diagnóstico , Neoplasias de Células Germinales y Embrionarias/patología , Neoplasias de Células Germinales y Embrionarias/terapia , Neoplasias Ováricas/diagnóstico , Neoplasias Ováricas/patología , Neoplasias Ováricas/terapia , Tasa de SupervivenciaRESUMEN
We present two new cases of Warsaw Breakage Syndrome (WABS), an autosomal recessive cohesinopathy, in sisters aged 13 and 11 years who both had compound heterozygous mutations in DDX11. After exclusion of Fanconi anemia, Bloom syndrome and Nijmegen breakage syndrome, whole exome sequencing revealed two novel variants-c.1523T>G, predicting (p.Leu508Arg) and c.1949-1G>A (IVS19-1G>A), that were confirmed with Sanger sequencing in both affected individuals. DDX11 encodes an iron-sulfur-containing DNA helicase, and mutations in this gene have been reported in the five WABS cases previously identified to date. The sisters reported here display the distinguishing clinical features of WABS: pre- and post-natal growth restriction, microcephaly, intellectual disability, sensorineural hearing loss with cochlear abnormalities, and facial dysmorphic features. In addition, our cases had early menarche at 8 and 10 years of age, bilateral small thumbs, and the younger, more severely affected sister had small fibulae. These findings broaden the WABS phenotype and the limb malformations demonstrate further clinical overlap with Fanconi anemia and other cohesinopathies, such as Roberts Syndrome.
Asunto(s)
ARN Helicasas DEAD-box/genética , ADN Helicasas/genética , Disgenesia Gonadal/genética , Pérdida Auditiva Sensorineural/genética , Discapacidad Intelectual/genética , Anomalías Múltiples/diagnóstico , Anomalías Múltiples/genética , Anomalías Múltiples/fisiopatología , Adolescente , Niño , Rotura Cromosómica , Anemia de Fanconi/diagnóstico , Anemia de Fanconi/genética , Anemia de Fanconi/fisiopatología , Femenino , Predisposición Genética a la Enfermedad , Disgenesia Gonadal/diagnóstico , Disgenesia Gonadal/fisiopatología , Pérdida Auditiva Sensorineural/diagnóstico , Pérdida Auditiva Sensorineural/fisiopatología , Humanos , Discapacidad Intelectual/diagnóstico , Discapacidad Intelectual/fisiopatología , Mutación , FenotipoRESUMEN
We report a novel syndromic disorder of sex development observed in three male siblings, presenting with the association of micropenis without hypospadias, cryptorchidism, very low level of antimüllerian hormone in the neonatal period, and no persistent müllerian duct structures, suggesting a progressive regression of testicular function. The patients described here showed a striking neurological involvement including bilateral periventricular cysts observed in the anterior part of the frontal horns prenatally and increasing in size and number over time, associated with infra and supratentorial parenchymal atrophy, dilated ventricular system, corpus callosum hypoplasia, severe intellectual disability, and epilepsy. Associated features included a distinctive facies, joint contractures, retinopathy, and hearing loss. Pathological examination was consistent with testicular dysgenesis and leukoencephalopathy with spongiosis and microcalcifications. To the best of our knowledge, this disease, characterized by a recognizable pattern of malformations, has not been previously reported. An exhaustive genetic and metabolic evaluation was normal. Autosomal recessive inheritance was considered to be likely, on the basis of SNP studies. We hope that the detailed description provided here of the clinical, radiological, and pathological findings observed in this family will help to identify further unrelated patients, and ultimately, to clarify the genetic basis of this condition. © 2017 Wiley Periodicals, Inc.
Asunto(s)
Trastornos del Desarrollo Sexual/diagnóstico , Trastornos del Desarrollo Sexual/genética , Disgenesia Gonadal/diagnóstico , Disgenesia Gonadal/genética , Leucoencefalopatías/diagnóstico , Leucoencefalopatías/genética , Fenotipo , Testículo/anomalías , Encéfalo/patología , Preescolar , Facies , Resultado Fatal , Enfermedades de los Genitales Masculinos/patología , Hormonas Esteroides Gonadales/sangre , Humanos , Recién Nacido , Imagen por Resonancia Magnética , Masculino , Pene/anomalías , Pene/patología , Hermanos , SíndromeRESUMEN
Recent studies on gonadal histology have improved the understanding of germ cell malignancy risk in patients with disorders of sex development (DSD), and evidence-based gonadal management strategies are gradually emerging. Especially in 46,XY DSD and 45,X/46,XY DSD, which are characterized by gonadal dysgenesis, the risk of germ cell malignancy is significantly increased. This paper summarized the progress over the past 10 years in malignancy risk assessment in patients with DSD, and its implications for optimal surgical handling of the involved gonads.
Asunto(s)
Disgenesia Gonadal/diagnóstico , Disgenesia Gonadal/cirugía , Niño , Árboles de Decisión , Femenino , Humanos , Masculino , Procedimientos Quirúrgicos Urológicos/métodosRESUMEN
Mutation of the Wilms tumor suppressor gene (WT1) has been recognized as one of the etiologies of steroid-resistant nephrotic syndrome (SRNS). The mutation is also responsible for gonadal dysgenesis in 46,XY individuals. Early recognition of the presence of Y chromosome is of particular importance because of the high risk of gonadal tumor. We present here three cases of steroid-resistant nephrotic syndrome with WT1 mutation and 46,XY karyotype. Patient 1 and 2 have intron splice site (IVS9+5G A) mutation. Patient 3 has c.1301GA (p. R434H) mutation. All cases had normal female external genitalia at birth and eluded the diagnosis of gonadal dysgenesis until later in life. We suggest that chromosomal analysis should be promptly performed in female patients with early-onset steroid-resistant nephrotic syndrome.â©.
Asunto(s)
Disgenesia Gonadal/genética , Síndrome Nefrótico/genética , Proteínas WT1/genética , Anomalías Múltiples , Adolescente , Preescolar , Resistencia a Medicamentos , Femenino , Disgenesia Gonadal/diagnóstico , Humanos , Lactante , Mutación , Síndrome Nefrótico/tratamiento farmacológico , EsteroidesRESUMEN
The co-occurrence of gonadal agenesis alongside hypoplastic derivatives of the paramesonephric ducts has rarely been observed. PATIENT(S): 16-year-old dizygotic twin sisters were referred to our department because of primary amenorrhea. X-ray, bone densitometry, ultrasonography, pelvic MRI and measurement of pituitary, ovary, and thyroid hormones were performed. Both twins showed hypergonadotropic hypogonadism, bilateral gonadal agenesis, fallopian tube, uterus, and vaginal hypoplasia but normal kidney and urinary tract structures and skeletal system. Analysis of Q-banded chromosomes in peripheral blood for the search for centromeric X-chromosome DNA and SRY gene was normal as well as the molecular analysis of FMR1, GDF9, and BMP15 genes. Estradiol gel was administered for one year followed by estroprogestin treatment. Both twins growth increased; breast development was stimulated and first menses occurred. Deregulation in the expression of the various HOX genes along the axis of the developing reproductive tract in a determinate time of development may be one of the mechanisms involved in the origin of this complex and rare association.
Asunto(s)
Amenorrea/diagnóstico , Disgenesia Gonadal/diagnóstico , Conductos Paramesonéfricos/anomalías , Adolescente , Amenorrea/congénito , Femenino , Humanos , Gemelos DicigóticosRESUMEN
Matthew-Wood syndrome (MWS), also termed Microphthalmia, syndrome 9 (MCOPS9, MIM 601186), Spear syndrome, or pulmonary hypoplasia, diaphragmatic hernia, anophthalmia and cardiac defects syndrome (PDAC syndrome), is an autosomal recessive disorder characterised by ocular, respiratory and cardiac abnormalities. Mutations in retinoic acid 6 gene (STRA6) have been reported in clinically diagnosed patients with MWS. Here we presented a case with MWS, who has characteristic findings of the syndrome as well as dextrocardia as an undescribed feature, and bilateral streak gonads which was described only in one patient previously. Molecular analysis showed a homozygous exonic missense mutation in the STRA6 gene.
Asunto(s)
Anoftalmos/genética , Dextrocardia/genética , Disgenesia Gonadal/genética , Enfermedades Pulmonares/genética , Proteínas de la Membrana/genética , Microftalmía/genética , Mutación Missense/genética , Anoftalmos/diagnóstico , Consanguinidad , Dextrocardia/diagnóstico , Exones/genética , Femenino , Disgenesia Gonadal/diagnóstico , Homocigoto , Humanos , Recién Nacido , Enfermedades Pulmonares/diagnóstico , Microftalmía/diagnóstico , Linaje , Embarazo , MortinatoAsunto(s)
Infecciones por Chlamydia , Chlamydia trachomatis/aislamiento & purificación , Trompas Uterinas , Disgenesia Gonadal , Laparoscopía/métodos , Ovario , Enfermedad Inflamatoria Pélvica , Adulto , Infecciones por Chlamydia/complicaciones , Infecciones por Chlamydia/diagnóstico , Infecciones por Chlamydia/fisiopatología , Trompas Uterinas/anomalías , Trompas Uterinas/diagnóstico por imagen , Femenino , Disgenesia Gonadal/complicaciones , Disgenesia Gonadal/diagnóstico , Disgenesia Gonadal/fisiopatología , Humanos , Ovario/anomalías , Ovario/diagnóstico por imagen , Enfermedad Inflamatoria Pélvica/complicaciones , Enfermedad Inflamatoria Pélvica/diagnóstico , Enfermedad Inflamatoria Pélvica/microbiología , Enfermedad Inflamatoria Pélvica/fisiopatología , Resultado del TratamientoRESUMEN
Puberty is a defining time of many adolescents' lives. It is a series of events that includes thelarche, pubarche, and menarche. Primary amenorrhea is the absence of menarche. There are numerous etiologies including outflow tract obstructions, gonadal dysgenesis, and anomalies of the hypothalamic axis. This review's aims are to define primary amenorrhea and describe the various causes, their workups, associated comorbidities, and treatment options. At the end, a generalist should be able to perform an assessment of an adolescent who presents with primary amenorrhea and, if warranted, begin initial treatment.
Asunto(s)
Amenorrea/etiología , Amenorrea/terapia , Genitales Femeninos/anomalías , Disgenesia Gonadal/complicaciones , Ovario/anomalías , Enfermedades de la Hipófisis/complicaciones , Adenohipófisis , Adolescente , Amenorrea/diagnóstico , Femenino , Disgenesia Gonadal/diagnóstico , Disgenesia Gonadal/terapia , Humanos , Enfermedades de la Hipófisis/diagnóstico , Enfermedades de la Hipófisis/terapia , Síndrome del Ovario Poliquístico/complicaciones , Síndrome del Ovario Poliquístico/terapiaRESUMEN
STUDY QUESTION: What is the optimal protocol of management for phenotypic female patients with Y chromosome or Y-derived sequences, in particular for adult patients? SUMMARY ANSWER: Immediate gonadectomy, long-term hormone therapy and psychological care are suggested to be the optimal management for older phenotypic female patients with Y chromosome or Y-derived sequences. WHAT IS KNOWN ALREADY: Phenotypic female patients with Y chromosome or Y-derived sequences are at increasing risk of developing gonadal tumors with age. Early diagnosis and safe guidelines of management for these patients are needed. STUDY DESIGN, SIZE, DURATION: One hundred and two phenotypic women with Y chromosome or Y-derived sequences were included in a straightforward, retrospective-observational study conducted over a period of 26 years from January 1985 to November 2010. PARTICIPANTS/MATERIALS, SETTING AND METHODS: Patients aged 16-34 years presenting to our Academic Department of Gynecology with symptoms of disorders of sex development were subjected to history taking, hormonal evaluation, conventional cytogenetic analysis, PCR, histopathology and immunohistochemistry. Features of the gonads were examined and the outcome of prophylactic gonadectomy evaluated. MAIN RESULTS AND THE ROLE OF CHANCE: Among the patients recruited in our study, 48 patients (47.1%) were diagnosed with complete/partial androgen insensitivity syndrome (CAIS/PAIS) (46XY), 33 cases (32.4%) with gonadal dysgenesis (46XY) and the remaining subjects (20.1%) with mixed gonadal dysgenesis (with sex chromosome structural abnormalities). The total incidence of malignancy was 17.6%. Seventeen patients (16.7%) had gonadoblastoma, while one patient (1.0%) with gonadal dysgenesis had dysgerminoma. Gonadoblastoma were observed in 2/21 patients with sex chromosome structural abnormalities (9.5%), 3/33 patients with gonadal dysgenesis (9.1%), 9/30 patients with CAIS (30.0%) and 3/18 patients with PAIS (16.7%). LIMITATIONS, REASONS FOR CAUTION: Selection bias in this cohort study may affect data interpretation due to the low incidence of disorders of sex development in the general population. WIDER IMPLICATIONS OF THE FINDINGS: The risk for malignant transformation may occur in early life and highly increase with age in patients with Y chromosome or Y-derived sequences. Optimal timing of gonadectomy should be decided by multiple factors including the subgroup of disorder, age and degree of patient's maturity. In addition, gonadal biopsy is suggested when the disease is diagnosed and any evidence of premalignancy warranties gonadectomy. STUDY FUNDING/COMPETING INTEREST(S): This work was supported by the National Key Scientific Research Project (2013CB967404), Natural Science Funds of Zhejiang Province (Y13H04005), Zhejiang Qianjiang talent plan (2013R10027), the Fundamental Research Funds for the Central Universities and Key Projects in the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period (2012BAI32B04). The authors have no conflicts of interest to declare. TRIAL REGISTRATION NUMBER None.
Asunto(s)
Cromosomas Humanos Y/ultraestructura , Trastornos Gonadales/genética , Gonadoblastoma/genética , Adolescente , Adulto , Síndrome de Resistencia Androgénica/diagnóstico , Síndrome de Resistencia Androgénica/genética , Aberraciones Cromosómicas , Citogenética , Femenino , Genitales/patología , Trastornos Gonadales/diagnóstico , Trastornos Gonadales/cirugía , Disgenesia Gonadal/diagnóstico , Disgenesia Gonadal/genética , Gonadoblastoma/diagnóstico , Gonadoblastoma/cirugía , Humanos , Inmunohistoquímica , Masculino , Fenotipo , Estudios Retrospectivos , Riesgo , Factores Sexuales , Adulto JovenRESUMEN
Malouf syndrome is a rare congenital disorder involving the heart, genitalia, skin and skeletal characteristics. In the present study, we report on the sporadic case of a young female with dilated cardiomyopathy, hypergonadotropic hypogonadism, a small chin, bilateral blepharoptosis, marfanoid elongated fingers and hypothyroidism. Malouf syndrome may be caused by heterozygous mutations in the lamin A/C (LMNA) gene. Genetic analyses and autopsy were performed. In spite of the patient's features, sequence analysis of the coding region of the LMNA gene including exon-intron boundaries identified only one benign polymorphism: homozygous silent variant 1698C>T (H566). There is a possibility that the sequence analysis may have not detected intronic mutations or mutations in portions of the 5'- and 3'-untranslated regions, which would confirm the clinical diagnosis.
Asunto(s)
Cardiomiopatía Dilatada/diagnóstico , Disgenesia Gonadal/diagnóstico , Hipogonadismo/diagnóstico , Insuficiencia Ovárica Primaria/diagnóstico , Adulto , Cardiomiopatía Dilatada/complicaciones , Cardiomiopatía Dilatada/etiología , Cardiomiopatía Dilatada/genética , Cardiomiopatía Dilatada/patología , Resultado Fatal , Femenino , Disgenesia Gonadal/complicaciones , Disgenesia Gonadal/etiología , Disgenesia Gonadal/genética , Disgenesia Gonadal/patología , Humanos , Hipogonadismo/complicaciones , Hipogonadismo/etiología , Hipogonadismo/genética , Hipogonadismo/patología , Lamina Tipo A/genética , Laminopatías , Mutación/genética , Insuficiencia Ovárica Primaria/etiologíaRESUMEN
Congenital uterine anomalies are often asymptomatic. They may present with infertility, recurrent miscarriage, preterm delivery, abnormal lie in pregnancy and other obstetric complications. We report the case of a 38-year old patient with unicornuate uterus without rudimentary horn and with unilateral left ovarian agenesis and unilateral left renal agenesis who gave birth to eleven children. Anomaly was incidentally diagnosed during laparoscopic sterilization.