RESUMEN
In insects, some sterols are essential not only for cell membrane homeostasis, but for biosynthesis of the steroid hormone ecdysone. Dietary sterols are required for insect development because insects cannot synthesize sterols de novo. Therefore, sterol-like compounds that can compete with essential sterols are good candidates for insect growth regulators. In this study, we investigated the effects of the plant-derived triterpenoids, cucurbitacin B and E (CucB and CucE) on the development of the fruit fly, Drosophila melanogaster. To reduce the effects of supply with an excess of sterols contained in food, we reared D. melanogaster larvae on low sterol food (LSF) with or without cucurbitacins. Most larvae raised on LSF without supplementation or with CucE died at the second or third larval instar (L2 or L3) stages, whereas CucB-administered larvae mostly died without molting. The developmental arrest caused by CucB was partially rescued by ecdysone supplementation. Furthermore, we examined the effects of CucB on larval-prepupal transition by transferring larvae from LSF supplemented with cholesterol to that with CucB just after the L2/L3 molt. L3 larvae raised on LSF with CucB failed to pupariate, with a remarkable developmental delay. Ecdysone supplementation rescued the developmental delay but did not rescue the pupariation defect. Furthermore, we cultured the steroidogenic organ, the prothoracic gland (PG) of the silkworm Bombyx mori, with or without cucurbitacin. Ecdysone production in the PG was reduced by incubation with CucB, but not with CucE. These results suggest that CucB acts not only as an antagonist of the ecdysone receptor as previously reported, but also acts as an inhibitor of ecdysone biosynthesis.
Asunto(s)
Drosophila melanogaster , Ecdisona , Triterpenos/farmacología , Animales , Bombyx/efectos de los fármacos , Bombyx/metabolismo , Proteínas de Drosophila/efectos de los fármacos , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/efectos de los fármacos , Drosophila melanogaster/crecimiento & desarrollo , Drosophila melanogaster/metabolismo , Ecdisona/antagonistas & inhibidores , Ecdisona/biosíntesis , Regulación del Desarrollo de la Expresión Génica , Hormonas Juveniles/farmacología , Larva/efectos de los fármacos , Larva/crecimiento & desarrollo , Larva/metabolismo , Metamorfosis Biológica/efectos de los fármacos , Muda/efectos de los fármacos , Técnicas de Cultivo de Órganos , Extractos Vegetales/farmacología , Pupa/efectos de los fármacos , Pupa/crecimiento & desarrollo , Pupa/metabolismoRESUMEN
With the rapid development and spread of resistance to insecticides among anopheline malaria vectors, the efficacy of current World Health Organization (WHO)-approved insecticides targeting these vectors is under threat. This has led to the development of novel interventions, including improved and enhanced insecticide formulations with new targets or synergists or with added sterilants and/or antimalarials, among others. To date, several studies in mosquitoes have revealed that the 20-hydroxyecdysone (20E) signaling pathway regulates both vector abundance and competence, two parameters that influence malaria transmission. Therefore, insecticides which target 20E signaling (e.g. methoxyfenozide and halofenozide) may be an asset for malaria vector control. While such insecticides are already commercially available for lepidopteran and coleopteran pests, they still need to be approved by the WHO for malaria vector control programs. Until recently, chemicals targeting 20E signaling were considered to be insect growth regulators, and their effect was mostly studied against immature mosquito stages. However, in the last few years, promising results have been obtained by applying methoxyfenozide or halofenozide (two compounds that boost 20E signaling) to Anopheles populations at different phases of their life-cycle. In addition, preliminary studies suggest that methoxyfenozide resistance is unstable, causing the insects substantial fitness costs, thereby potentially circumventing one of the biggest challenges faced by current vector control efforts. In this review, we first describe the 20E signaling pathway in mosquitoes and then summarize the mechanisms whereby 20E signaling regulates the physiological processes associated with vector competence and vector abundance. Finally, we discuss the potential of using chemicals targeting 20E signaling to control malaria vectors.
Asunto(s)
Anopheles , Ecdisona , Hormonas Juveniles/farmacología , Mosquitos Vectores , Animales , Anopheles/efectos de los fármacos , Anopheles/fisiología , Ecdisona/antagonistas & inhibidores , Ecdisona/metabolismo , Ecdisterona/farmacología , Humanos , Resistencia a los Insecticidas/efectos de los fármacos , Insecticidas/farmacología , Estadios del Ciclo de Vida/efectos de los fármacos , Malaria/transmisión , Control de Mosquitos/métodos , Mosquitos Vectores/efectos de los fármacos , Mosquitos Vectores/fisiología , Transducción de Señal/efectos de los fármacosRESUMEN
BACKGROUND: Dibenzoylhydrazine analogues have been developed successfully as a new group of insect growth regulators, called ecdysone agonists or moulting accelerating compounds. A notable feature is their high activity against lepidopteran insects, raising the question as to whether species-specific analogues can be isolated. In this study, the specificity of ecdysone agonists was addressed through a comparative analysis in two important lepidopterans, the silkworm Bombyx mori L. and the cotton leafworm Spodoptera littoralis (Boisd.). RESULTS: When collections of non-steroidal ecdysone agonists containing different mother structures (dibenzoylhydrazine, acylaminoketone, tetrahydroquinoline) were tested, in vitro reporter assays showed minor differences using cell lines derived from both species. However, when compounds with high ecdysone agonist activity were examined in toxicity assays, larvicidal activity differed considerably. Of note was the identification of three dibenzoylhydrazine analogues with > 100-fold higher activity against Bombyx than against Spodoptera larvae. CONCLUSION: The present study demonstrated that species-specific ecdysone-agonist-based insecticides can be developed, but their species specificity is not based on differences in the activation of the ecdysone receptor but rather on unidentified in vivo parameters such as permeability of the cuticle, uptake/excretion by the gut or metabolic detoxification.
Asunto(s)
Bombyx/efectos de los fármacos , Genes Reporteros/genética , Hormonas Juveniles/toxicidad , Muda/efectos de los fármacos , Spodoptera/efectos de los fármacos , Pruebas de Toxicidad/métodos , Animales , Bombyx/metabolismo , Línea Celular , Ecdisona/agonistas , Ecdisona/antagonistas & inhibidores , Hidrazinas/toxicidad , Larva/efectos de los fármacos , Receptores de Esteroides/metabolismo , Especificidad de la Especie , Spodoptera/metabolismoRESUMEN
C-type lectins participate in pathogen recognition and other defense responses in innate immunity as well as in cell-cell interactions. A new cDNA encoding a 335-residue polypeptide containing two tandem C-type lectin domains was cloned from the haemocytes of Helicoverpa armigera (Ha-lectin). Northern hybridizations revealed that the mRNA of Ha-lectin was expressed constitutively in haemocytes, and was up-regulated following injections of bacteria, yeast, or virus. Ha-lectin expression was also induced in the fat body when larvae were injected with bacteria, yeast or 20-hydroxyecdysone and a non-steroidal ecdysone agonist, RH-2485. The Ha-lectin was detected in granular haemocytes. The recombinant protein (rHa-lectin) expressed in Escherichia coli had hemagglutinating and sugar-binding activities. The native Ha-lectin protein was identified in haemocytes and plasma using a polyclonal antiserum raised against rHa-lectin by immunoblotting techniques. All together, our results suggest that the Ha-lectin gene is involved in innate immunity, and may also participate in the molting process.
Asunto(s)
Hemocitos/metabolismo , Lectinas Tipo C/metabolismo , Mariposas Nocturnas/metabolismo , Secuencia de Aminoácidos , Animales , Clonación Molecular , Ecdisona/antagonistas & inhibidores , Ecdisona/farmacología , Ecdisterona/farmacología , Hidrazinas/farmacología , Hormonas Juveniles/farmacología , Larva/metabolismo , Lectinas Tipo C/genética , Datos de Secuencia Molecular , Mariposas Nocturnas/efectos de los fármacos , Mariposas Nocturnas/genéticaRESUMEN
In holometabolous insects such as mosquito, Aedes aegypti, midgut undergoes remodeling during metamorphosis. Insect metamorphosis is regulated by several hormones including juvenile hormone (JH) and 20-hydroxyecdysone (20E). The cellular and molecular events that occur during midgut remodeling were investigated by studying nuclear stained whole mounts and cross-sections of midguts and by monitoring the mRNA levels of genes involved in 20E action in methoprene-treated and untreated Ae. aegypti. We used JH analog, methoprene, to mimic JH action. In Ae. aegypti larvae, the programmed cell death (PCD) of larval midgut cells and the proliferation and differentiation of imaginal cells were initiated at about 36h after ecdysis to the 4th instar larval stage (AEFL) and were completed by 12h after ecdysis to the pupal stage (AEPS). In methoprene-treated larvae, the proliferation and differentiation of imaginal cells was initiated at 36h AEFL, but the PCD was initiated only after ecdysis to the pupal stage. However, the terminal events that occur for completion of PCD during pupal stage were blocked. As a result, the pupae developed from methoprene-treated larvae contained two midgut epithelial layers until they died during the pupal stage. Quantitative PCR analyses showed that methoprene affected midgut remodeling by modulating the expression of ecdysone receptor B, ultraspiracle A, broad complex, E93, ftz-f1, dronc and drice, the genes that are shown to play key roles in 20E action and PCD. Thus, JH analog, methoprene acts on Ae. aegypti by interfering with the expression of genes involved in 20E action resulting in a block in midgut remodeling and death during pupal stage.
Asunto(s)
Aedes/crecimiento & desarrollo , Ecdisona/fisiología , Tracto Gastrointestinal/crecimiento & desarrollo , Insecticidas/farmacología , Metopreno/farmacología , Aedes/efectos de los fármacos , Animales , Ecdisona/antagonistas & inhibidores , Tracto Gastrointestinal/efectos de los fármacos , Larva/efectos de los fármacos , Pupa/efectos de los fármacosRESUMEN
In this paper we investigate in vivo and in vitro effects of orally administered azadirachtin and ecdysone on the phagocytic responses of Rhodnius prolixus 5th-instar larval hemocytes to the yeast Saccharomyces cerevisiae. Groups of insects fed non-treated blood (control) and insects that received azadirachtin plus ecdysone in the blood meal were inoculated with yeast cells in the hemocele. The injected yeast cells disappeared rapidly from the hemolymph, being removed completely by 90min after inoculation. In the insects treated only with azadirachtin the clearance of free yeast circulating particles was significantly delayed compared to the two previously mentioned groups. It was demonstrated that the binding of yeast cells to hemocytes was reduced in the insects treated only with azadirachtin in comparison to both non-treated control and azadirachtin plus ecdysone-treated groups. Phagocytosis occurred when yeast cells were added to hemocyte monolayers prepared with hemolymph from blood fed insects, treated or not with azadirachtin plus ecdysone, so that yeast cells were rapidly bound to hemocytes and internalized in high numbers. By contrast, insects treated with azadirachtin exhibited a drastic reduction in the quantity of yeast cell-hemocyte binding and subsequent internalization. In all groups, the hemocytes attached to the glass slides were predominantly plasmatocytes. The magnitude and speed of the cellular response suggests that hemocyte phagocytosis is one of the main driving forces for the clearance of free circulating yeast cells from the hemolymph. We propose that ecdysone modulates phagocytosis in R. prolixus larvae, and that this effect is antagonized by azadirachtin.
Asunto(s)
Ecdisona/inmunología , Hemocitos/inmunología , Fagocitosis/inmunología , Rhodnius/inmunología , Saccharomyces cerevisiae/fisiología , Animales , Ecdisona/antagonistas & inhibidores , Inmunidad Celular , Insecticidas/farmacología , Larva/inmunología , Limoninas/farmacología , Rhodnius/microbiologíaRESUMEN
The steroidal moulting hormones (ecdysteroids) mediate developmental transitions in insects, and their regulation is mainly controlled by the production and inactivation of these steroid hormones at the appropriate developmental times. One route of metabolism of ecdysteroids in insects involves EO (ecdysone oxidase)-catalysed conversion into 3-dehydroecdysteroid, which undergoes reduction to the corresponding 3-epiecdysteroid. By a twin-stranded bioinformatics approach, employing both phylogenomics and model structure-based analysis, we first predicted that DmEO (the EO of Drosophila melanogaster) corresponds to the protein product of gene CG9504. When CG9504 was expressed in COS7 cells, significant conversion of ecdysone into 3-dehydroecdysone was observed. Quantitative PCR and enzyme assay showed that DmEO was mainly expressed in the midgut during the late instars at a time corresponding to a hormone titre peak. DmEO shares only 27% amino acid sequence identity with Spodoptera littoralis (Lepidoptera) EO, yet key substrate-binding residues are well conserved. A model of DmEO is consistent with an inability to catalyse reaction of cholesterol derivatives. The significance of DmEO in ligand activation is discussed in relation to new evidence suggesting that 3-dehydro- and 3-epiecdysteroids may be functionally active as ligands in a novel, atypical ecdysteroid signalling pathway involving the Drosophila orphan nuclear receptor, DHR38, rather than being merely hormone inactivation products.
Asunto(s)
3-Hidroxiesteroide Deshidrogenasas/química , 3-Hidroxiesteroide Deshidrogenasas/metabolismo , Drosophila melanogaster/enzimología , Drosophila melanogaster/crecimiento & desarrollo , Regulación del Desarrollo de la Expresión Génica/fisiología , 3-Hidroxiesteroide Deshidrogenasas/genética , Secuencia de Aminoácidos , Animales , Benzoatos/farmacología , Sitios de Unión , Ecdisona/análogos & derivados , Ecdisona/antagonistas & inhibidores , Ecdisona/química , Ecdisona/metabolismo , Regulación Enzimológica de la Expresión Génica , Hidrazinas/farmacología , Larva/efectos de los fármacos , Larva/enzimología , Larva/genética , Larva/metabolismo , Datos de Secuencia Molecular , Filogenia , Unión Proteica , Pupa/enzimología , Pupa/metabolismo , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Transducción de Señal , Distribución TisularRESUMEN
Many plant compounds are able to modulate herbivore growth and reproduction by directly interacting with steroid hormones systems. In insects, several classes of phytochemicals, including brassinosteroids and related substances, interfere with molting and reproduction. The effects of the molting-hormone antagonist pesticide DI-31, a brassinosteroid analogue, on Aedes aegypti mosquito larvae were tested in two different exposure scenarios. After static exposure of first-instar larvae, the calculated NOEC, LOEC, and LC(50) values referenced to 19 d were 0.03, 0.036, and 0.04 mg/mL, respectively. Semistatic exposure of fourth-instar larvae revealed them to be slightly less susceptible than the younger larvae (NOEC 0.03 mg/mL, LOEC 0.036 mg/mL, LC(50) 0.049 mg/mL referenced to 19 days). In both cases mortality was immediate and larval development was retarded. This study suggests that A. aegypti could be a useful model for the detection of hormonally active substances such as DI-31.
Asunto(s)
Ecdisona/antagonistas & inhibidores , Plaguicidas/toxicidad , Esteroides/toxicidad , Aedes , Animales , Bioensayo , Monitoreo del Ambiente/métodos , Larva/crecimiento & desarrollo , Dosificación Letal MedianaRESUMEN
For normal development of Manduca sexta larvae, the ecdysteroid titer must drop following its sudden rise at the start of the molting cycle; this sudden decline in titer may be due to myoinhibitory peptide I (MIP I), which has an inhibitory effect on the release of ecdysone by the prothoracic glands of Bombyx mori in vitro. Using an antiserum to MIP, we have demonstrated secretion of an MIP-like peptide by the epiproctodeal glands of Manduca sexta, which are located on each proctodeal nerve, just anterior to the rectum. These MIP-immunoreactive glands are also present in B. mori. In fourth-instar larvae of M. sexta, the epiproctodeal glands show a distinct cycle of synthesis and sudden release of MIP that coincides with the time of the rapid decline in ecdysteroid titer. The function of the epiproctodeal glands appears to be the timely release of MIP during the molting cycle, so as to inhibit the prothoracic glands and thus to facilitate the sudden decline in ecdysteroid titer. In addition, we have found that MIP immunoreactivity is co-localized with that of crustacean cardioactive peptide (CCAP) in the 704 interneurons; these peptides appear to be co-released at the time of ecdysis. It is known that CCAP can initiate the ecdysis motor program; our results suggest that MIP may also be involved in activating ecdysis behavior.
Asunto(s)
Bombyx/fisiología , Ecdisona/antagonistas & inhibidores , Larva/fisiología , Manduca/fisiología , Muda , Péptidos/farmacología , Animales , Inmunohistoquímica , Técnicas In Vitro , Larva/crecimiento & desarrollo , Microscopía Confocal , Neurosecreción , Péptidos/metabolismoRESUMEN
The role of ecdysteroids in crustacean embryo development and the susceptibility of the developing embryo to the antiecdysteroidal properties of an environmental chemical were evaluated. The agricultural fungicide fenarimol was shown to exhibit antiecdysteroidal activity to the crustacean Daphnia magna by lowering endogenous ecdysone levels and delaying molting in a concentration-dependent fashion that was mitigated by co-exposure to exogenous 20-hydroxyecdysone. Exposure of either gravid maternal organisms or isolated embryos to fenarimol resulted in embryo abnormalities ranging from early partial developmental arrest to incomplete development of antennae and shell spines. Developmental abnormalities were associated with suppressed ecdysone levels in the embryos and the abnormalities could be prevented by co-exposure to 20-hydroxyecdysone. Developmental abnormalities caused by the antiecdysteroid were associated with reduced fecundity of the parental organisms. These results demonstrate that ecdysteroids are critical to normal crustacean embryo development and environmental antiecdysteroids can disrupt normal embryo development and compromise the production of viable offspring. Antiecdysteroidal activity may provide a means by which environmental chemicals impact crustacean species while not affecting vertebrates.
Asunto(s)
Daphnia/embriología , Ecdisona/antagonistas & inhibidores , Muda/fisiología , Animales , Anomalías Congénitas/fisiopatología , Anomalías Congénitas/veterinaria , Femenino , Fertilidad , Fungicidas Industriales/farmacología , Pirimidinas/farmacologíaRESUMEN
At the onset of Drosophila metamorphosis, the steroid hormone 20-OH ecdysone directly induces a small number of early puffs in the polytene chromosomes of the larval salivary gland. Proteins encoded by the early genes corresponding to these transcriptional puffs then regulate the activity of both the early puffs themselves and a much larger set of late puffs. Three of these early genes encode transcription factors that play critical regulatory roles during metamorphosis. Here we report the cloning, DNA sequence, genomic structure, ecdysone inducibility, and temporal expression of an early gene residing in the 23E early puff and denoted E23 (Early gene at 23). In contrast to other early genes, E23 encodes a protein with similarity to ATP-binding cassette transporters. Using heat shock-inducible transgenes, we found that E23 overexpression not only produces phenotypic abnormalities and lethality, but also interferes with ecdysone-mediated gene activation, demonstrating that E23 is capable of modulating the ecdysone response. Our results suggest the existence of a previously unrecognized regulatory mechanism for modulating steroid hormone signaling in Drosophila.
Asunto(s)
Transportadoras de Casetes de Unión a ATP/metabolismo , Proteínas de Drosophila , Drosophila melanogaster/genética , Ecdisona/antagonistas & inhibidores , Genes de Insecto/genética , Proteínas de Insectos/metabolismo , Metamorfosis Biológica/genética , Activación Transcripcional , Transportadoras de Casetes de Unión a ATP/química , Transportadoras de Casetes de Unión a ATP/genética , Secuencia de Aminoácidos , Animales , Clonación Molecular , Drosophila melanogaster/efectos de los fármacos , Drosophila melanogaster/crecimiento & desarrollo , Drosophila melanogaster/metabolismo , Ecdisona/farmacología , Perfilación de la Expresión Génica , Genes Letales/genética , Proteínas de Insectos/química , Proteínas de Insectos/genética , Larva/efectos de los fármacos , Larva/genética , Metamorfosis Biológica/efectos de los fármacos , Datos de Secuencia Molecular , Familia de Multigenes/genética , Fenotipo , Pupa/efectos de los fármacos , Pupa/genética , ARN Mensajero/genética , ARN Mensajero/metabolismo , Alineación de Secuencia , Transcripción Genética/efectos de los fármacos , Transcripción Genética/genética , Activación Transcripcional/efectos de los fármacosRESUMEN
APP is a precursor of beta amyloid deposited in Alzheimer's disease (AD). Although genetic studies established that mutations in APP cause familial AD (FAD), the mechanism for neuronal death by FAD mutants has not been well understood. We established neuronal cells (F11/EcR/V642I cells) in which V642I APP was inducibly expressed by ecdysone. Treatment with ecdysone, but not vehicle, killed most cells within a few days, with rounding, shrinkage, and detachment as well as nuclear fragmentation. Death was suppressed by Ac-DEVD-CHO and pertussis toxin. Electron microscopic analysis revealed that apoptosis occurred in ecdysone-treated cells. V642I-APP-induced death was suppressed by the anti-AD factors estrogen and apoE2. These data demonstrate not only that expression of this FAD gene causes neuronal apoptosis, but that F11/EcR/V642I cells, the first neuronal cells with inducible FAD gene expression, provide a useful model system in investigating AD disorders.
Asunto(s)
Enfermedad de Alzheimer/metabolismo , Precursor de Proteína beta-Amiloide/genética , Precursor de Proteína beta-Amiloide/metabolismo , Apoptosis/genética , Neuronas/metabolismo , Enfermedad de Alzheimer/genética , Clorometilcetonas de Aminoácidos/farmacología , Sustitución de Aminoácidos , Precursor de Proteína beta-Amiloide/farmacología , Animales , Apolipoproteína E2 , Apolipoproteína E4 , Apolipoproteínas E/farmacología , Apoptosis/efectos de los fármacos , Línea Celular , Supervivencia Celular/efectos de los fármacos , Supervivencia Celular/genética , Inhibidores de Cisteína Proteinasa/farmacología , Relación Dosis-Respuesta a Droga , Ecdisona/antagonistas & inhibidores , Ecdisona/farmacología , Estradiol/farmacología , Expresión Génica/efectos de los fármacos , Células Híbridas , Ratones , Modelos Biológicos , Neuronas/efectos de los fármacos , Neuronas/ultraestructura , Oligopéptidos/farmacología , Toxina del Pertussis , Ratas , Receptores de Esteroides/biosíntesis , Proteínas Recombinantes/biosíntesis , Transfección , Factores de Virulencia de Bordetella/farmacologíaRESUMEN
Insect molting hormone (ecdysteroid) inactivation occurs by several routes, including 26-hydroxylation and further oxidation to the 26-oic acids. Thus, the ecdysteroid 26-hydroxylase is a critical enzyme involved in precise regulation of ecdysteroid titers during insect development. Administration of the ecdysteroid agonist, RH-5849 (1,2-dibenzoyl, 1-tert-butyl hydrazone), or 20-hydroxyecdysone to the tobacco hornworm, Manduca sexta, results in induction of ecdysteroid 26-hydroxylase activity in midgut mitochondria and microsomes. The biochemical and kinetic properties of the ecdysteroid 26-hydroxylase were investigated. The mitochondrial enzyme was found to have optimal activity at a pH of 7. 5 in a Hepes or sodium phosphate buffer at 30-37 degrees C. The apparent K(m) of the microsomal 26-hydroxylase for 20-hydroxyecdysone substrate was lower than that of the mitochondrial enzyme for either 20-hydroxyecdysone or ecdysone substrate. The V(max) of the 26-hydroxylase in both subcellular fractions was slightly higher using 20-hydroxyecdysone as substrate compared to ecdysone. Demonstration that activity of the mitochondrial 26-hydroxylase was inhibited by incubation in a CO (or N(2)) atmosphere, taken together with the requirement for reducing cofactor and the efficacy of the P450 inhibitors, ketoconazole and fenarimol, provided strong evidence that the hydroxylase is cytochrome P450-dependent. Indirect evidence suggested that the mitochondrial and microsomal ecdysteroid 26-hydroxylase(s) could exist in a less active dephosphorylated state or more active phosphorylated state. Using Escherichia coli alkaline phosphatase to remove covalently bound phosphate groups, the activity of the 26-hydroxylase was decreased and, conversely, activity was enhanced using a cAMP-dependent protein kinase with appropriate cofactors. In addition, the protein kinase was shown to reactivate the 26-hydroxylase activity in alkaline phosphatase-treated fractions.
Asunto(s)
Ecdisona/antagonistas & inhibidores , Ecdisona/metabolismo , Manduca/enzimología , Esteroide Hidroxilasas/metabolismo , Nucleótidos de Adenina/metabolismo , Fosfatasa Alcalina/metabolismo , Animales , Monóxido de Carbono/metabolismo , Monóxido de Carbono/farmacología , Colestanotriol 26-Monooxigenasa , Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidores , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Inhibidores Enzimáticos del Citocromo P-450 , Sistema Enzimático del Citocromo P-450/metabolismo , Ecdisterona/metabolismo , Activación Enzimática , Concentración de Iones de Hidrógeno , Cinética , Manduca/citología , Microsomas/enzimología , Mitocondrias/enzimología , Nitrógeno/metabolismo , Nitrógeno/farmacología , Oxígeno/metabolismo , Oxígeno/farmacología , Fosforilación , Cianuro de Potasio/farmacología , Esteroide Hidroxilasas/antagonistas & inhibidores , TemperaturaRESUMEN
In crustaceans, production of molting hormones (or ecdysteroids) by the molting glands (Y-organs; YO), is under negative control exerted by a neuropeptide, the molt-inhibiting hormone (MIH). MIH of the crab Carcinus maenas inhibits in vitro steroidogenesis of basal (intermolt crab) or activated (premolt crab) YO. MIH inhibits secretion of the two ecdysteroids synthesized by crab YO, ecdysone (E) secreted throughout the molting cycle, and 25-deoxyecdysone (25dE), secreted during the premolt period. At a MIH concentration of 10(-8) M, E is reduced about 50% and 25dE 94%. Regardless of the molting stage, this inhibition of steroidogenesis is reversible, dose dependent and measurable after 5 min. On intermolt YO, MIH induced cGMP increase and 8BrcGMP mimics the effect of MIH: at this stage cGMP seems to be involved with MIH inhibition of steroidogenesis. On premolt YO MIH induced a transient increase of cAMP (2-fold) and a long-lasting enhancement of cGMP (60-fold). On active YO, we demonstrated that a low concentration (10(-5) M) of dbcAMP, 8BrcAMP, 8BrcGMP, or agents increasing intracellular cAMP, mimic MIH effects and inhibit steroidogenesis. From these observations it is concluded that both cyclic nucleotides are involved in the mode of action of MIH on activated YO. At this premolt period, MIH/cAMP may act cooperatively with MIH/cGMP in the inhibitory control of steroidogenesis by crab YO.
Asunto(s)
Braquiuros/metabolismo , AMP Cíclico/fisiología , GMP Cíclico/fisiología , Ecdisona/antagonistas & inhibidores , Hormonas de Invertebrados/metabolismo , 1-Metil-3-Isobutilxantina/farmacología , 8-Bromo Monofosfato de Adenosina Cíclica/farmacología , Animales , Braquiuros/fisiología , Bucladesina/farmacología , Colforsina/farmacología , GMP Cíclico/análogos & derivados , GMP Cíclico/farmacología , GMP Dibutiril Cíclico/farmacología , Relación Dosis-Respuesta a Droga , Ecdisona/biosíntesis , Ecdisona/metabolismo , Hormonas de Invertebrados/farmacologíaRESUMEN
The search for antitumor compounds from Southeast Asian plants led to ponasterones, the first phytoecdysteroids, just a year after structure determination of ecdysone and 20-hydroxyecdysone. An independent study of Chinese herb constituents by Takemoto et al. at Tohoku University led to the simultaneous and totally independent discovery of phytoecdysteroids. These findings greatly facilitated research in insect and crustacean physiology. The original structural studies on various phytoecdysteroids have led to interdisciplinary bioorganic studies in the area related to ecdysone receptor, ecdysone biosynthetic precursor (or its storage form), crustacean molt inhibitory factors, and so on.
Asunto(s)
Ecdisterona/análogos & derivados , Plantas/química , Animales , Crustáceos , Ecdisona/antagonistas & inhibidores , Ecdisona/química , Ecdisterona/química , Ecdisterona/historia , Ecdisterona/aislamiento & purificación , Historia del Siglo XXRESUMEN
Attempts to isolate the molt-inhibiting hormone (MIH) of crustaceans from crab eyestalks (ES) resulted in the characterization of xanthurenic acid as an inhibitor of ecdysone biosynthesis in the cultured Y-organ-complex (YOC) homogenate. It was also found that 3-hydroxy-L-kynurenine present in the ES is transformed into xanthurenic acid in the YOC and body fluid. Its mode of inhibitory action in ecdysone biosynthesis is probably inactivation of cytochrome P-450.