RESUMEN
BACKGROUND: Bladder cancer is one of the most common malignancies but the corresponding diagnostic methods are either invasive or limited in specificity and/or sensitivity. This study aimed to develop a urine-based methylation panel for bladder cancer detection by improving published panels and validate performance of the new panel with clinical samples. METHODS: Related researches were reviewed and 19 potential panels were selected. RRBS was performed on a cohort with 45 samples to reassess these panels and a new panel inherited best markers was developed. The new panel was applied with qMSP platform to 33 samples from the RRBS cohort and the results were compared to those of RRBS. Lastly, another larger cohort with 207 samples was used to validate new panel performance with qMSP. RESULTS: Three biomarkers (PCDH17, POU4F2 and PENK) were selected to construct a new panel P3. P3 panel achieved 100% specificity and 71% sensitivity with RRBS in corresponding cohort and then showed a better performance of 100% specificity and 84% sensitivity with qMSP platforms in a balanced cohort. When validated with 207-sample cohort, P3 with qMSP showed a performance of 97% specificity and 87% sensitivity which was modestly improved compared to the panels it derided from. CONCLUSIONS: Overall, the P3 panel achieved relatively high sensitivity and accuracy in bladder cancer detection.
Asunto(s)
Metilación de ADN , Detección Precoz del Cáncer/métodos , Urinálisis/métodos , Neoplasias de la Vejiga Urinaria/diagnóstico , Orina/química , Anciano , Anciano de 80 o más Años , Biomarcadores de Tumor/genética , Biomarcadores de Tumor/orina , Cadherinas/orina , Encefalinas/orina , Femenino , Humanos , Masculino , Persona de Mediana Edad , Precursores de Proteínas/orina , Sensibilidad y Especificidad , Factor de Transcripción Brn-3B/orinaAsunto(s)
Lesión Renal Aguda/diagnóstico , Biomarcadores/análisis , Sistemas de Atención de Punto/tendencias , Lesión Renal Aguda/prevención & control , Biomarcadores/orina , Encefalinas/análisis , Encefalinas/orina , Humanos , Proteínas de Unión a Factor de Crecimiento Similar a la Insulina/análisis , Proteínas de Unión a Factor de Crecimiento Similar a la Insulina/orina , Unidades de Cuidados Intensivos/organización & administración , Precursores de Proteínas/análisis , Precursores de Proteínas/orina , Inhibidor Tisular de Metaloproteinasa-2/análisis , Inhibidor Tisular de Metaloproteinasa-2/orinaRESUMEN
Leu-enkephalin, its stable analogue [D-Ala2-D-Leu5]-enkephalin and the C-fragment of lipotropin (beta endorphin) injected intravenously in the rat produced antidiuretic responses which were inhibited reversibly by naloxone. It was shown for Leu-enkephalin that injection into the cerebral ventricles was at least ten times more effective than intravenous injection and for [D-Ala2-D-Leu5]-enkephalin that the antidiuretic response was associated with increased excretion of vaspressin in the urine.
