RESUMEN
Enzymes that degrade ß-glucan play important roles in various industries, including those related to brewing, animal feed, and health care. Csph16A, an endo-ß-1,3(4)-glucanase encoded by a gene from the halotolerant, xerotolerant, and radiotrophic black fungus Cladosporium sphaerospermum, was cloned and expressed in Pichia pastoris. Two isoforms (Csph16A.1 and Csph16A.2) are produced, arising from differential glycosylation. The proteins were predicted to contain a catalytic Lam16A domain, along with a C-terminal domain (CTD) of unknown function which exhibits minimal secondary structure. Employing PCR-mediated gene truncation, the CTD of Csph16A was excised to assess its functional impact on the enzyme and determine potential alterations in biotechnologically relevant characteristics. The truncated mutant, Csph16A-ΔC, exhibited significantly enhanced thermal stability at 50°C, with D-values 14.8 and 23.5 times greater than those of Csph16A.1 and Csph16A.2, respectively. Moreover, Csph16A-ΔC demonstrated a 20%-25% increase in halotolerance at 1.25 and 1.5 M NaCl, respectively, compared to the full-length enzymes. Notably, specific activity against cereal ß-glucan, lichenan, and curdlan was increased by up to 238%. This study represents the first characterization of a glucanase from the stress-tolerant fungus C. sphaerospermum and the first report of a halotolerant and engineered endo-ß-1,3(4)-glucanase. Additionally, it sheds light on a group of endo-ß-1,3(4)-glucanases from Antarctic rock-inhabiting black fungi harboring a Lam16A catalytic domain and a novel CTD of unknown function.
Asunto(s)
Estabilidad de Enzimas , beta-Glucanos , beta-Glucanos/metabolismo , Cladosporium/enzimología , Cladosporium/genética , Dominios Proteicos , Proteínas Fúngicas/genética , Proteínas Fúngicas/química , Proteínas Fúngicas/metabolismo , Endo-1,3(4)-beta-Glucanasa/genética , Endo-1,3(4)-beta-Glucanasa/metabolismo , Endo-1,3(4)-beta-Glucanasa/química , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Clonación Molecular , Temperatura , SaccharomycetalesRESUMEN
A novel glycoside hydrolase (GH) family 16 multi-domain ß-1,3-1,4-glucanase (FsGlc16A) from Fibrobacter sp. UWP2 was identified, heterogeneously expressed, and its enzymatic properties, protein structure and application potential were characterized. Enzymological characterization showed that FsGlc16A performed the optimal catalytic activity at pH 4.5 and 50 °C with a specific activity of 3263 U/mg. FsGlc16A exhibited the substrate specificity towards oat ß-glucan, barley ß-glucan and lichenan, and in addition, it hydrolyzed oat ß-glucan and lichenan into different ß-glucooligosaccharides with polymerization degrees of 3-4, which further illustrated that it belonged to the endo-type ß-1,3-1,4-glucanase. FsGlc16A was classified in subfamily25 of GH16. A 'PXSSSS' repeats domain was identified at the C-terminus of FsGlc16A, which was distinct from the typical GH family 16 ß-1,3-1,4-glucanases. Removing the 'PXSSSS' repeats domain affected the binding of the substrate to FsGlc16A and reduced the enzyme activity. FsGlc16A displayed good potential for the applications, which hydrolyzed oat bran into ß-glucooligosaccharides, and reduced filtration time (18.89 %) and viscosity (3.64 %) in the saccharification process. This study investigated the enzymatic properties and domain function of FsGlc16A, providing new ideas and insights into the study of ß-1,3-1,4-glucanase.
Asunto(s)
Glucanos , Especificidad por Sustrato , Hidrólisis , Glucanos/química , Glucanos/metabolismo , Concentración de Iones de Hidrógeno , Secuencia de Aminoácidos , Temperatura , Dominios Proteicos , beta-Glucanos/metabolismo , beta-Glucanos/química , Glicósido Hidrolasas/química , Glicósido Hidrolasas/metabolismo , Cinética , Endo-1,3(4)-beta-Glucanasa/química , Endo-1,3(4)-beta-Glucanasa/metabolismo , Endo-1,3(4)-beta-Glucanasa/genética , Clonación Molecular , Filogenia , Estabilidad de EnzimasRESUMEN
ß-1,3-glucanases are the main digestive enzymes of plant and fungal cell wall. Transcriptomic analysis of the fungus-growing termite Macrotermes barneyi revealed a high expression of a predicted ß-1,3(4)-glucanase (Mbbgl) transcript in termite gut. Here, we described the cDNA cloning, heterologous expression, and enzyme characterization of Mbbgl. Sequence analysis and RT-PCR results showed that Mbbgl is a termite-origin GH16 ß-1,3(4)-glucanase. The recombinant enzyme showed the highest activity towards laminarin and was active optimally at 50 °C, pH 5.5. The enzyme displayed endo/exo ß-1,3(4)-glucanase activities. Moreover, Mbbgl had weak transglycosylation activity. The results indicate that Mbbgl is an endogenous digestive ß-1,3(4)-glucanase, which contributes to the decomposition of plant biomass and fungal hyphae. Additionally, the multiple activities, pH, and ion stabilities make Mbbgl a potential candidate for application in the food industry.
Asunto(s)
Digestión , Endo-1,3(4)-beta-Glucanasa/metabolismo , Hongos/crecimiento & desarrollo , Isópteros/enzimología , Isópteros/microbiología , Animales , Clonación Molecular , Endo-1,3(4)-beta-Glucanasa/química , Endo-1,3(4)-beta-Glucanasa/genética , Estabilidad de Enzimas , Glicosilación , Concentración de Iones de Hidrógeno , Especificidad por Sustrato , TemperaturaRESUMEN
Bacterial ß-1,3-1,4-glucanase (BG) is an endoglucanase that hydrolyzes linear ß-glucans containing ß-1,3 and ß-1,4 linkages, such as barley ß-glucans. In this study, a BG gene was transformed into the food-grade plasmid pNZ8149 and successfully expressed in Lactococcus lactis NZ3900 using the nisin-controlled gene expression system. To facilitate extracellular secretion, the signal peptide Usp45 was added during vector construction. A histidine tag was also added for affinity purification. BG was extracellularly secreted and was also present in the cells in soluble form. N-terminal amino acid residue analysis of secreted BG revealed that the Usp45 peptide was removed. The optimum temperature and pH for both intracellular and extracellular BG were 40⯰C and 6, respectively. The enzyme kinetic parameters, Vmax, Km, kcat, and kcat/Km, of extracellular BG were 1317.51⯵molâ¯min-1, 1.97â¯mgâ¯ml-1, 588.54 s-1, and 298.26â¯mlâ¯s-1âmg-1, respectively. There was no significant difference in the enzyme kinetic parameters of intracellular and extracellular BG. The growth pattern of transformed L. lactis NZ3900 in ß-glucan-containing liquid medium confirmed ß-glucan degradation by BG. The transformed strain degraded ß-glucans, produced gluco-oligosaccharide, and produced lactic acid. The strain and expression system constructed in this study could be applied to industrial fields requiring BG produced in food-grade lactococcal secretory expression system.
Asunto(s)
Bacillus/enzimología , Proteínas Bacterianas/genética , Endo-1,3(4)-beta-Glucanasa/genética , Alimentos Fermentados/microbiología , Peces/microbiología , Expresión Génica , Lactococcus lactis/genética , Animales , Bacillus/genética , Bacillus/aislamiento & purificación , Proteínas Bacterianas/química , Proteínas Bacterianas/metabolismo , Clonación Molecular , Endo-1,3(4)-beta-Glucanasa/química , Endo-1,3(4)-beta-Glucanasa/metabolismo , Estabilidad de Enzimas , Cinética , Lactococcus lactis/metabolismo , beta-Glucanos/metabolismoRESUMEN
ß-1,3-glucanases, the plant PR-2 family of pathogenesis-related (PR) proteins, can be constitutively expressed and induced in wheat crop to enhance its anti-fungal pathogen defense. This study aimed to investigate the inhibitory effect of wheat ß-1,3-glucanase on fungi most commonly associated with wheat kernel. A ß-1,3-glucanase from wheat was successfully expressed in Pichia pastoris X-33 and its biochemical and antifungal properties were characterized herein. The molecular weight of recombinant ß-1,3-glucanase is approximately 33 kDa. ß-1,3-glucanase displays optimal activity at pH 6.5, remaining relatively high at pH 5.5-8.0. The optimal reaction temperature of ß-1,3-glucanase is 50⯰C, retaining approximately 84.0% residual activity after heat-treated at 50⯰C for 1â¯h. The steady-state kinetic parameters of ß-1,3-glucanase against laminarin was determined and the Km and Vmax were 1.32⯱â¯0.20â¯mg/ml and 96.4⯱â¯4.4 Uâ¯mg-1 protein, respectively. The inhibitory effect of purified ß-1,3-glucanase against the seven fungi commonly associated with wheat kernel was assessed in vitro. ß-1,3-glucanase exerted differential inhibitory effects on hyphal growth of Fusarium graminearum, Alternaria sp., A. glaucus, A. flavus, A. niger, and Penicillium sp. Spore formation and mycelial morphology of Alternaria sp., A. flavus, and A. niger were significantly affected by ß-1,3-glucanase (1U). The present results would help elucidate the mechanism underlying the inhibition of wheat ß-1,3-glucanases on pathogens.
Asunto(s)
Antifúngicos , Endo-1,3(4)-beta-Glucanasa , Hongos Mitospóricos/crecimiento & desarrollo , Proteínas de Plantas , Triticum , Antifúngicos/química , Antifúngicos/aislamiento & purificación , Antifúngicos/farmacología , Endo-1,3(4)-beta-Glucanasa/química , Endo-1,3(4)-beta-Glucanasa/genética , Endo-1,3(4)-beta-Glucanasa/aislamiento & purificación , Endo-1,3(4)-beta-Glucanasa/farmacología , Calor , Concentración de Iones de Hidrógeno , Pichia/enzimología , Pichia/genética , Proteínas de Plantas/química , Proteínas de Plantas/genética , Proteínas de Plantas/aislamiento & purificación , Proteínas de Plantas/farmacología , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/farmacología , Triticum/enzimología , Triticum/genéticaRESUMEN
To resolve the structure of laminarin, the recombinant endo-ß-1,3-glucanase from Coprinopsis cinerea, which has specific activity on ß-1,3 glycosidic bond and could hydrolyze the laminarin with complex structure, was used to hydrolyze laminarin. Then, the structures of enzyme-resistant oligosaccharides were quantitatively and qualitatively analysed by high-performance anion exchange chromatography coupled with mass spectrometry. The laminarin from Laminaria digitata contains 9.51% ß-1,6 glycosidic bonds only in the branches (branch degree 7.68%). The laminarin from Eisenia bicyclis contains more ß-1,6 glycosidic bonds: 19.42% ß-1,6 glycosidic bonds in backbone and more and longer ß-1,6 branches (branch degree 25.99%). The differences in the ratio of glycosidic bonds and branch degree influence their bioactivity: the antioxidant activity and the antimicrobial activity against Gram positive bacteria of the laminarin from E. bicyclis is stronger than the laminarin from L. digitata, but the antimicrobial activity on Gram negative bacteria of the laminarin from E. bicyclis is weaker.
Asunto(s)
Antioxidantes/metabolismo , Agentes de Control Biológico/química , Agentes de Control Biológico/metabolismo , Endo-1,3(4)-beta-Glucanasa/química , Endo-1,3(4)-beta-Glucanasa/metabolismo , Glucanos/metabolismo , Agaricales/enzimología , Antioxidantes/química , Cromatografía Líquida de Alta Presión , Cromatografía por Intercambio Iónico , Clonación Molecular , Endo-1,3(4)-beta-Glucanasa/genética , Glucanos/química , Espectrometría de Masas en TándemRESUMEN
A novel endo-ß-1,3-1,4-glucanase gene (PbBglu16A) was cloned from Paenibacillus barengoltzii and heterogeneously expressed in Escherichia coli. The recombinant ß-1,3-1,4-glucanase (PbBglu16A) was purified to homogeneity with a recovery yield of 78.6% and a specific activity of 431.8Umg-1. The molecular mass of PbBglu16A was estimated to be 44.0kDa by SDS-PAGE. The optimal pH and temperature of PbBglu16A were 6.0 and 55°C, respectively. The enzyme was stable within pH 3.5-9.0 and up to 55°C. PbBglu16A exhibited high substrate specificity towards barley ß-glucan, oat ß-glucan and lichenin. PbBglu16A showed an endo-type cleavage pattern and hydrolyzed endogenous enzyme-deactivated oat bran into ß-gluco-oligosaccharides with a yield of 7.0%, which mainly consisted of trioligosaccharide and tetraoligosaccharide. Further, PbBglu16A could promote mashing with a reduced filtration time (14.0%) and viscosity (3.4%). Thus, PbBglu16A might be a promising candidate for the production of ß-gluco-oligosaccharides and in brewing industry.
Asunto(s)
Endo-1,3(4)-beta-Glucanasa/química , Paenibacillus/enzimología , beta-Glucanos/metabolismo , Clonación Molecular , Electroforesis en Gel de Poliacrilamida , Estabilidad de Enzimas , Escherichia coli , Concentración de Iones de Hidrógeno , Especificidad por SustratoRESUMEN
The aim of this study was to improve the stability of ß-1,3-1,4-glucanase by substituting hydrophobic residue for specific amino acid. The results indicated that the catalytic efficiency, thermostability and halostability were enhanced simultaneously by replacement of Lys48 with Ala (K48A) or Leu (K48L). Comparison of kinetic parameters revealed that catalytic efficiency of mutants is enhanced as a result of the increase in substrate affinity. A great improvement in thermostability and halostability was observed. The half-lives of mutants significantly increased (up to â¼7-fold) at 60-70°C. Moreover, relative enzymatic activities of mutants were observed more than 80% even in the presence of 30% NaCl, and half-lives were increased to 3-fold that of wild-type. Based on above results, when applied to ionic liquid, mutants were more active and stable compared to wild-type. These were the results from improvement of protein functions by the substitution of hydrophobic single residue in adjacent with forming carbohydrate binding cavity. Therefore, this report could be helpful for improvement of the enzyme property and for biotechnological application as well.
Asunto(s)
Alanina/química , Bacillus/química , Proteínas Bacterianas/química , Endo-1,3(4)-beta-Glucanasa/química , Leucina/química , Lisina/química , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Bacillus/enzimología , Proteínas Bacterianas/genética , Biocatálisis , Endo-1,3(4)-beta-Glucanasa/genética , Estabilidad de Enzimas , Calor , Interacciones Hidrofóbicas e Hidrofílicas , Líquidos Iónicos/química , Cinética , Mutagénesis Sitio-Dirigida , Mutación , Alineación de Secuencia , Cloruro de Sodio/química , Especificidad por SustratoRESUMEN
A gene coding endo-ß-1,3(4)-glucanase (ENG16A) was cloned from Coprinopsis cinerea and heterologously expressed in Pichia pastoris. ENG16A only acts on substrates containing ß-1,3 glycosidic bonds but not on substrates containing only ß-1,4- or ß-1,6-glycosidic bonds. Interestingly, compared to the activity of this enzyme towards carboxymethyl (CM)-pachyman containing only ß-1,3-glycosidic bonds, its activity towards barley ß-glucan containing both ß-1,3-glycosidic and ß-1,4-glycosidic bonds was increased by 64.72 %,, its activity towards laminarin containing both ß-1,3-glycosidic and ß-1,6-glycosidic bonds was decreased by 50.83 %. In addition, ENG16A has a higher Km value and Vmax for barley ß-glucan than laminarin, which may be related to the fact that barley ß-glucan contains mainly ß-1,4-glycosidic bonds mixed with a few ß-1,3-glycosidic bonds, whereas laminarin mainly contains ß-1,3-glycosidic bonds with a few ß-1,6-branched glucose residues. The adjacent ß-1,4-glycosidic bond promotes ENG16A to hydrolyse ß-1,3-glycosidic bonds, leading to an increased Vmax; the nearby ß-1,6-glycosidic bonds inhibited its hydrolysis of ß-1,3-glycosidic bonds, resulting in a decreased Vmax. This property is suggested to be related to the mechanism that C. cinerea uses to degrade and utilize hemicellulose in straw medium and to protect its cell wall during the mycelium growth stage.
Asunto(s)
Agaricales/enzimología , Endo-1,3(4)-beta-Glucanasa/metabolismo , Agaricales/genética , Clonación Molecular , Endo-1,3(4)-beta-Glucanasa/química , Endo-1,3(4)-beta-Glucanasa/genética , Expresión Génica , Pichia/genética , Pichia/metabolismo , Especificidad por SustratoRESUMEN
BACKGROUND: Error-prone polymerase chain reaction (PCR) is frequently used in directed evolution of enzymes to modify their quality. In this study, error-prone PCR was used to improve the catalytic efficiency of ß-1,3-1,4-glucanase from Bacillus altitudinis YC-9. RESULTS: By screening, the mutant Glu-3060 with higher activity was selected among 5000 transformants. After induction with isopropyl ß-D-1-thiogalactopyranoside (IPTG), the activity of the mutant Glu-3060 reached 474.6 U mL(-1), resulting in a 48.6% increment of the parent enzyme activity. Research on the characterization of the mutated enzyme showed the optimal pH of the mutated enzyme to be 5.0, which is lower than the parent enzyme, but thermal stability was almost the same between them. Sequence analysis of the mutated enzyme revealed that three amino acids were changed compared with the parent enzyme, including K142N, Q203L and N214D. CONCLUSION: The three-dimensional structure predicted by SWISS-MODEL of the mutated enzyme Glu-3060 showed that the substitution of three amino acids had an effect on the catalytic activity, stability and optimal pH of the enzyme, through changing the charge properties or electron density, forming secondary keys, the acidity of the amino acids and the side chain group. The sum effects of all the factors were increased activity of the mutated enzyme and decreased optimal pH, while the same thermostability was maintained, thereby increasing the suitability of the enzyme for industrial use.
Asunto(s)
Secuencia de Aminoácidos , Bacillus/enzimología , Endo-1,3(4)-beta-Glucanasa/genética , Mutación , Ingeniería de Proteínas , Bacillus/genética , Catálisis , Clonación Molecular , Endo-1,3(4)-beta-Glucanasa/química , Estabilidad de Enzimas , Calor , Concentración de Iones de Hidrógeno , Reacción en Cadena de la Polimerasa/métodos , Conformación ProteicaRESUMEN
BACKGROUND: In China, abalone (Haliotis discus hannai) production is growing annually. During industrial processing, the viscera, which are abundant of cellulase, are usually discarded or processed into low-value feedstuff. Thus, it is of interest to obtain cellulase from abalone viscera and investigate its application for preparation of functional oligosaccharides. RESULTS: A cellulase was purified from the hepatopancreas of abalone by ammonium sulfate precipitation and two-steps column chromatography. The molecular weight of the cellulase was 45 kDa on SDS-PAGE. Peptide mass fingerprinting analysis yielded 103 amino acid residues, which were identical to cellulases from other species of abalone. Substrate specificity analysis indicated that the cellulase is an endo-1,4-ß-glucanase. Hydrolysis of seaweed Porphyra haitanensis polysaccharides by the enzyme produced oligosaccharides with degree of polymerisation of two to four, whose monosaccharide composition was 58% galactose, 4% glucose and 38% xylose. The oligosaccharides revealed 2,2'-diphenyl-1-picrylhydrazyl free radical as well as hydrogen peroxide scavenging activity. CONCLUSION: It is feasible and meaningful to utilise cellulase from the viscera of abalone for preparation of functional oligosaccharides. © 2015 Society of Chemical Industry.
Asunto(s)
Antioxidantes/aislamiento & purificación , Endo-1,3(4)-beta-Glucanasa/aislamiento & purificación , Gastrópodos/enzimología , Oligosacáridos/aislamiento & purificación , Porphyra/química , Algas Marinas/química , Vísceras/enzimología , Secuencia de Aminoácidos , Animales , Antioxidantes/química , Antioxidantes/economía , Antioxidantes/metabolismo , Acuicultura/economía , Secuencia de Carbohidratos , China , Endo-1,3(4)-beta-Glucanasa/química , Endo-1,3(4)-beta-Glucanasa/economía , Endo-1,3(4)-beta-Glucanasa/metabolismo , Estabilidad de Enzimas , Estudios de Factibilidad , Conservantes de Alimentos/química , Conservantes de Alimentos/economía , Conservantes de Alimentos/aislamiento & purificación , Conservantes de Alimentos/metabolismo , Hepatopáncreas/enzimología , Concentración de Iones de Hidrógeno , Hidrólisis , Residuos Industriales/análisis , Residuos Industriales/economía , Peso Molecular , Oligosacáridos/química , Oligosacáridos/economía , Oligosacáridos/metabolismo , Especificidad por Sustrato , TemperaturaRESUMEN
The recent classification of glycoside hydrolase family 5 (GH5) members into subfamilies enhances the prediction of substrate specificity by phylogenetic analysis. However, the small number of well characterized members is a current limitation to understanding the molecular basis of the diverse specificity observed across individual GH5 subfamilies. GH5 subfamily 4 (GH5_4) is one of the largest, with known activities comprising (carboxymethyl)cellulases, mixed-linkage endo-glucanases, and endo-xyloglucanases. Through detailed structure-function analysis, we have revisited the characterization of a classic GH5_4 carboxymethylcellulase, PbGH5A (also known as Orf4, carboxymethylcellulase, and Cel5A), from the symbiotic rumen Bacteroidetes Prevotella bryantii B14. We demonstrate that carboxymethylcellulose and phosphoric acid-swollen cellulose are in fact relatively poor substrates for PbGH5A, which instead exhibits clear primary specificity for the plant storage and cell wall polysaccharide, mixed-linkage ß-glucan. Significant activity toward the plant cell wall polysaccharide xyloglucan was also observed. Determination of PbGH5A crystal structures in the apo-form and in complex with (xylo)glucan oligosaccharides and an active-site affinity label, together with detailed kinetic analysis using a variety of well defined oligosaccharide substrates, revealed the structural determinants of polysaccharide substrate specificity. In particular, this analysis highlighted the PbGH5A active-site motifs that engender predominant mixed-linkage endo-glucanase activity vis à vis predominant endo-xyloglucanases in GH5_4. However the detailed phylogenetic analysis of GH5_4 members did not delineate particular clades of enzymes sharing these sequence motifs; the phylogeny was instead dominated by bacterial taxonomy. Nonetheless, our results provide key enzyme functional and structural reference data for future bioinformatics analyses of (meta)genomes to elucidate the biology of complex gut ecosystems.
Asunto(s)
Proteínas Bacterianas/metabolismo , Celulasa/metabolismo , Endo-1,3(4)-beta-Glucanasa/metabolismo , Glicósido Hidrolasas/metabolismo , Modelos Moleculares , Prevotella/enzimología , Sustitución de Aminoácidos , Apoenzimas/antagonistas & inhibidores , Apoenzimas/química , Apoenzimas/genética , Apoenzimas/metabolismo , Proteínas Bacterianas/antagonistas & inhibidores , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Biocatálisis , Dominio Catalítico , Celulasa/antagonistas & inhibidores , Celulasa/química , Celulasa/genética , Celulosa/química , Celulosa/metabolismo , Endo-1,3(4)-beta-Glucanasa/antagonistas & inhibidores , Endo-1,3(4)-beta-Glucanasa/química , Endo-1,3(4)-beta-Glucanasa/genética , Inhibidores Enzimáticos/química , Inhibidores Enzimáticos/metabolismo , Inhibidores Enzimáticos/farmacología , Glucanos/química , Glucanos/metabolismo , Glicósido Hidrolasas/antagonistas & inhibidores , Glicósido Hidrolasas/química , Glicósido Hidrolasas/genética , Calor , Concentración de Iones de Hidrógeno , Mutación , Filogenia , Conformación Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Especificidad por Sustrato , Xilanos/química , Xilanos/metabolismoRESUMEN
Xyloglucan-specific endo-ß-1,4-glucanases (Xegs, EC 3.2.1.151) exhibit high catalytic specificity for ß-1,4 linkages of xyloglucan, a branched hemicellulosic polysaccharide abundant in dicot primary cell walls and present in many monocot species. In nature, GH12 Xegs are not associated with carbohydrate-binding modules (CBMs), and here, we have investigated the effect of the fusion of the xyloglucan-specific CBM44 on the structure and function of a GH12 Xeg from Aspergillus niveus (XegA). This fusion presented enhanced catalytic properties and conferred superior thermal stability on the XegA. An increased k cat (chimera, 177.03 s(-1); XegA, 144.31 s(-1)) and reduced KM (chimera, 1.30 mg mL(-1); XegA, 1.50 mg mL(-1)) resulted in a 1.3-fold increase in catalytic efficiency of the chimera over the parental XegA. Although both parental and chimeric enzymes presented catalytic optima at pH 5.5 and 60 °C, the thermostabilitiy of the chimera at 60 °C was greater than the parental XegA. Moreover, the crystallographic structure of XegA together with small-angle X-ray scattering (SAXS) and molecular dynamics simulations revealed that the spatial arrangement of the domains in the chimeric enzyme resulted in the formation of an extended binding cleft that may explain the improved kinetic properties of the CBM44-XegA chimera.
Asunto(s)
Aspergillus/enzimología , Endo-1,3(4)-beta-Glucanasa/química , Endo-1,3(4)-beta-Glucanasa/metabolismo , Proteínas Fúngicas/química , Proteínas Fúngicas/metabolismo , Glucanos/metabolismo , Xilanos/metabolismo , Secuencia de Aminoácidos , Aspergillus/química , Aspergillus/genética , Endo-1,3(4)-beta-Glucanasa/genética , Proteínas Fúngicas/genética , Glucanos/química , Cinética , Simulación de Dinámica Molecular , Datos de Secuencia Molecular , Ingeniería de Proteínas , Estructura Terciaria de Proteína , Dispersión del Ángulo Pequeño , Especificidad por Sustrato , Difracción de Rayos X , Xilanos/químicaRESUMEN
Plant ß-1,3-glucanases are members of the pathogenesis-related protein 2 (PR-2) family, which is one of the 17 PR protein families and plays important roles in biotic and abiotic stress responses. One of the differentially expressed proteins (spot 842) identified in a recent proteomic comparison between five pairs of closely related maize (Zea mays L.) lines differing in aflatoxin resistance was further investigated in the present study. Here, the corresponding cDNA was cloned from maize and designated as ZmGns. ZmGns encodes a protein of 338 amino acids containing a potential signal peptide. The expression of ZmGns was detectible in all tissues studied with the highest level in silks. ZmGns was significantly induced by biotic stresses including three bacteria and the fungus Aspergillus flavus. ZmGns was also induced by most abiotic stresses tested and growth hormones including salicylic acid. In vivo, ZmGns showed a significant inhibitory activity against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 and fungal pathogen Botrytis cinerea when it overexpressed in Arabidopsis. Its high level of expression in the silk tissue and its induced expression by phytohormone treatment, as well as by bacterial and fungal infections, suggest it plays a complex role in maize growth, development, and defense.
Asunto(s)
Antiinfecciosos/farmacología , Endo-1,3(4)-beta-Glucanasa/genética , Estrés Fisiológico/efectos de los fármacos , Zea mays/enzimología , Secuencia de Aminoácidos , Antifúngicos/farmacología , Arabidopsis/genética , Arabidopsis/microbiología , Aspergillus/efectos de los fármacos , Botrytis/efectos de los fármacos , Clonación Molecular , Endo-1,3(4)-beta-Glucanasa/química , Endo-1,3(4)-beta-Glucanasa/metabolismo , Escherichia coli/efectos de los fármacos , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Genes de Plantas , Concentración de Iones de Hidrógeno , Datos de Secuencia Molecular , Especificidad de Órganos/efectos de los fármacos , Especificidad de Órganos/genética , Filogenia , Enfermedades de las Plantas/genética , Enfermedades de las Plantas/microbiología , Reguladores del Crecimiento de las Plantas/farmacología , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/enzimología , Hojas de la Planta/genética , Plantas Modificadas Genéticamente , Proteínas Recombinantes/metabolismo , Ácido Salicílico/farmacología , Alineación de Secuencia , Análisis de Secuencia de ADN , Especificidad por Sustrato/efectos de los fármacos , Temperatura , Zea mays/efectos de los fármacos , Zea mays/genética , Zea mays/microbiologíaRESUMEN
1,3-1,4-ß-Glucanase received great interest due to its application in brewing and feed industries. Application of 1,3-1,4-ß-glucanase in brewing industry helps make up for the defect that plant-derived ß-glucanases are heat-sensitive. A new strain, CGX5-1, exhibited remarkable 1,3-1,4-ß-glucanase, was isolated from Asian giant hornet nest and identified Bacillus tequilensis. Moreover, a new 1,3-1,4-ß-glucanase gene from B. tequilensis was cloned and measured to be 720 bp encoding 239 amino acids, with a predicted molecular weight of 26.9 kDa. After expressed in Escherichia coli BL21, active recombinant enzyme of 24 kDa was detected in the supernatant of cell culture, with the activity of 2,978.2 U/mL. The new enzyme was stable in the pH 5.0-7.5 with the highest activity measured at pH 6.0. Moreover, it is thermostable within 45 to 60 °C. The property of the new recombinant enzyme makes this enzyme a broad prospect in brewing industry. Moreover, this is the first report on 1,3-1,4-ß-glucanase produced by B. tequilensis.
Asunto(s)
Bacillus/enzimología , Proteínas Bacterianas , Endo-1,3(4)-beta-Glucanasa , Bacillus/genética , Proteínas Bacterianas/biosíntesis , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Proteínas Bacterianas/aislamiento & purificación , Endo-1,3(4)-beta-Glucanasa/biosíntesis , Endo-1,3(4)-beta-Glucanasa/química , Endo-1,3(4)-beta-Glucanasa/genética , Endo-1,3(4)-beta-Glucanasa/aislamiento & purificación , Escherichia coli/genética , Escherichia coli/metabolismo , Expresión Génica , Calor , Concentración de Iones de Hidrógeno , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificaciónRESUMEN
A new ß-1,3-1,4-glucanase gene (PlicA) was cloned from Paenibacillus sp. S09. The ORF contained 717 bp coding for a 238 amino acid protein. PlicA, expressed in Escherichia coli and purified by Ni(2+)-affinity chromatography, had optimum activity at 55 °C and pH 6.2. The specific activity toward barley ß-glucan reached 7,055 U/mg. K m and V max values with barley ß-glucan were 3.7 mg/ml and 3.3 × 10(3) µmol/min mg, respectively. The enzyme exhibited acid- and alkali-tolerance with more than 80 % activity remaining after incubation for 4 h at pH 3.5-12. PlicA was salt-tolerant (>90 % activity retained in 4 M NaCl at 25 °C for 24 h) and salt-activated: activity rising 1.5-fold in 0.5 M NaCl. The thermostability was improved by NaCl and CaCl2. This is the first report of an acid-, alkali- and salt-tolerant bacterial ß-1,3-1,4-glucanase with high catalytic efficiency.
Asunto(s)
Endo-1,3(4)-beta-Glucanasa/aislamiento & purificación , Endo-1,3(4)-beta-Glucanasa/metabolismo , Inhibidores Enzimáticos/metabolismo , Paenibacillus/enzimología , Ácidos/metabolismo , Álcalis/metabolismo , Cromatografía de Afinidad , Clonación Molecular , ADN Bacteriano/química , ADN Bacteriano/genética , Endo-1,3(4)-beta-Glucanasa/química , Endo-1,3(4)-beta-Glucanasa/genética , Estabilidad de Enzimas , Escherichia coli/enzimología , Escherichia coli/genética , Expresión Génica , Concentración de Iones de Hidrógeno , Cinética , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Paenibacillus/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Sales (Química)/metabolismo , Análisis de Secuencia de ADN , Temperatura , beta-Glucanos/metabolismoRESUMEN
A bifunctional enzyme has been created by fusing two Bacillus subtilis enzymes: the ß-1,3-1,4-glucanase (BglS, EC 3.2.1.73) that hydrolyzes plant cell wall ß-glucans and the copper-dependent oxidase laccase (CotA, EC 1.10.3.2) that catalyzes the oxidation of aromatic compounds with simultaneous reduction of oxygen to water. The chimeric laccase/ß-1,3-1,4-glucanase was created by insertion fusion of the bglS and cotA genes, and expressed in Escherichia coli. The affinity-purified recombinant chimeric enzyme showed both laccase and glucanase activities, with a maximum laccase activity at pH 4.5 and 75°C that showed a V(max) 30% higher than observed for the parental laccase. The maximum glucanase activity in the chimeric enzyme was at pH 6.0 and 50°C, with a slight reduction in V(max) by â¼10% compared with the parental glucanase. A decreased K(M) resulted in an overall increase in the K(cat)/K(M) value for the glucanase activity of the chimeric enzyme. The hydrolytic activity of the chimera was 20% higher against natural milled sugarcane bagasse as compared with equimolar mixtures of the separate parental enzymes. Molecular dynamics simulations indicated the approximation of the two catalytic domains in the chimeric enzyme, and the formation of an inter-domain interface may underlie the improved catalytic function.
Asunto(s)
Bacillus subtilis/enzimología , Celulosa/metabolismo , Endo-1,3(4)-beta-Glucanasa/metabolismo , Lacasa/metabolismo , Ingeniería de Proteínas/métodos , Saccharum/metabolismo , Celulosa/química , Endo-1,3(4)-beta-Glucanasa/química , Endo-1,3(4)-beta-Glucanasa/genética , Cinética , Lacasa/química , Lacasa/genética , Simulación de Dinámica Molecular , Oxidación-Reducción , Conformación Proteica , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Saccharum/químicaRESUMEN
Four types of ß-1,3-1,4 glucanase (ß-glucanase, EC 3.2.1.73) genes, designated bglA13, bglA16, bglA51, and bglM2, were found in the cDNA library of Neocallimastix patriciarum J11. All were highly homologous with each other and demonstrated a close phylogenetic relationship with and a similar codon bias to Streptococcus equinus. The presence of expansion and several predicted secondary structures in the 3' untranslated regions (3'UTRs) of bglA16 and bglM2 suggest that these two genes were duplicated recently, whereas bglA13 and bglA16, which contain very short 3'UTRs, were replicated earlier. These findings indicate that the ß-glucanase genes from N. patriciarum J11 may have arisen by horizontal transfer from the bacterium and subsequent duplication in the rumen fungus. ß-Glucanase genes of Streptococcus equinus, Ruminococcus albus 7, and N. patriciarum J11 were cloned and expressed by Escherichia coli. The recombinant ß-glucanases cloned from S. equinus, R. albus 7, and N. patriciarum J11 were endo-acting and had similar substrate specificity, but they demonstrated different properties in other tests. The specific activities and catalytic efficiency of the bacterial ß-glucanases were also significantly lower than those of the fungal ß-glucanases. Our results also revealed that the activities and some characteristics of enzymes were changed during the horizontal gene transfer event. The specific activities of the fungal ß-glucanases ranged from 26,529 to 41,209 U/mg of protein when barley-derived ß-glucan was used as the substrate. They also demonstrated similar pH and temperature optima, substrate specificity, substrate affinity, and hydrolysis patterns. Nevertheless, BglA16 and BglM2, two recently duplicated ß-glucanases, showed much higher k(cat) values than others. These results support the notion that duplicated ß-glucanase genes, namely, bglA16 and bglM2, increase the reaction efficiency of ß-glucanases and suggest that the catalytic efficiency of ß-glucanase is likely to be a criterion determining the evolutionary fate of duplicate forms in N. patriciarum J11.
Asunto(s)
Endo-1,3(4)-beta-Glucanasa/metabolismo , Neocallimastix/enzimología , Clonación Molecular , ADN Bacteriano/química , ADN Bacteriano/genética , ADN de Hongos/química , ADN de Hongos/genética , Endo-1,3(4)-beta-Glucanasa/química , Endo-1,3(4)-beta-Glucanasa/genética , Estabilidad de Enzimas , Escherichia coli/enzimología , Escherichia coli/genética , Concentración de Iones de Hidrógeno , Cinética , Datos de Secuencia Molecular , Neocallimastix/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Ruminococcus/enzimología , Ruminococcus/genética , Análisis de Secuencia de ADN , Streptococcus/enzimología , Streptococcus/genética , Especificidad por Sustrato , TemperaturaRESUMEN
To fulfill the need for acid-tolerant and thermostable ß-1,3-1,4-glucanases, an error-prone PCR and DNA-shuffling approach was employed to enhance the activity of thermostable ß-1,3-1,4-glucanases from Paecilomyces thermophila (PtLic16A) at acidic pH. Mutant PtLic16AM2 was selected and characterized, and showed optimal activity at pH 5.0, corresponding to an acidic shift of 2.0 pH units relative to the wild-type enzyme. Other properties of PtLic16A such as temperature optimum and substrate specificity that are beneficial for industrial applications did not change. Based on the substituted residues of PtLic16AM2, three site-directed mutations, D56G, D221G and C263S, were designed to study these residues' roles. The amino acid residues at positions 56 and 263 were found to be important in determining optimal pH activity. Activity of the D221G variant showed no significant difference from the wild-type. Thus, it appears that the change in optimal pH for PtLic16AM2 was mainly caused by the combination of substitutions D56G and C263S. This study provides a ß-1,3-1,4-glucanase (PtLic16AM2) with high potential for industrial applications.
Asunto(s)
Endo-1,3(4)-beta-Glucanasa/genética , Endo-1,3(4)-beta-Glucanasa/metabolismo , Paecilomyces/enzimología , Paecilomyces/genética , Evolución Molecular Dirigida , Endo-1,3(4)-beta-Glucanasa/química , Estabilidad de Enzimas , Concentración de Iones de Hidrógeno , Modelos Moleculares , Mutagénesis Sitio-Dirigida , Mutación , TemperaturaRESUMEN
1,3-1,4-ß-D-Glucanase has been widely used as a feed additive to help non-ruminant animals digest plant fibers, with potential in increasing nutrition turnover rate and reducing sanitary problems. Engineering of enzymes for better thermostability is of great importance because it not only can broaden their industrial applications, but also facilitate exploring the mechanism of enzyme stability from structural point of view. To obtain enzyme with higher thermostability and specific activity, structure-based rational design was carried out in this study. Eleven mutants of Fibrobacter succinogenes 1,3-1,4-ß-D-glucanase were constructed in attempt to improve the enzyme properties. In particular, the crude proteins expressed in Pichia pastoris were examined firstly to ensure that the protein productions meet the need for industrial fermentation. The crude protein of V18Y mutant showed a 2 °C increment of Tm and W203Y showed â¼30% increment of the specific activity. To further investigate the structure-function relationship, some mutants were expressed and purified from P. pastoris and Escherichia coli. Notably, the specific activity of purified W203Y which was expressed in E. coli was 63% higher than the wild-type protein. The double mutant V18Y/W203Y showed the same increments of Tm and specific activity as the single mutants did. When expressed and purified from E. coli, V18Y/W203Y showed similar pattern of thermostability increment and 75% higher specific activity. Furthermore, the apo-form and substrate complex structures of V18Y/W203Y were solved by X-ray crystallography. Analyzing protein structure of V18Y/W203Y helps elucidate how the mutations could enhance the protein stability and enzyme activity.