RESUMEN
Abortion caused by the parasite Neospora caninum is an important threat to the livestock industry worldwide. Trophoblasts and caruncular cells play major roles in initiating innate immune responses and controlling parasite infection at the fetal-maternal interface. In the present study, bovine uterine epithelial cells (BUECs) and bovine trophoblastic (BT) cells treated with bovine interferon-gamma (IFN-γ), IFN-alpha (IFN-α) and IFN-tau (IFN-τ) followed by infection with N. caninum were examined by measuring the mRNA expression levels of numerous pregnancy-associated proteins and observing parasite growth to elucidate the host-parasite interaction at the uteroplacental region. N. caninum infection increased the expression of prolactin-related protein 1 (PRP1), pregnancy-associated glycoprotein 1 (PAG1), and cytokines (TNF-α, IL-8 and IL-10) in BUECs and of IL-8 in BT cells. Bovine IFN-γ inhibited IL-8 and TNF-α expression in BUECs and IL-8 in BT cells. In contrast, the expression of the interferon-stimulated gene OAS1 was significantly increased by treatment of the infected BT cells with IFN-γ. However, treatment with bovine IFNs did not inhibit N. caninum growth in either cell line. In conclusion, our results suggest that bovine IFN-γ plays a crucial role in control of pathogenesis in uterus and induction of inflammatory response in the placental region following N. caninum infection, rather than growth inhibition of the parasites.
Asunto(s)
Coccidiosis , Citocinas , Endometrio , Células Epiteliales , Neospora , Proteínas Gestacionales , Trofoblastos , Animales , Bovinos , Neospora/fisiología , Trofoblastos/parasitología , Trofoblastos/metabolismo , Femenino , Citocinas/metabolismo , Citocinas/genética , Células Epiteliales/parasitología , Endometrio/parasitología , Endometrio/metabolismo , Endometrio/citología , Coccidiosis/parasitología , Coccidiosis/veterinaria , Proteínas Gestacionales/genética , Proteínas Gestacionales/farmacología , Embarazo , Enfermedades de los Bovinos/parasitología , Regulación de la Expresión Génica , Interacciones Huésped-ParásitosRESUMEN
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Asunto(s)
Humanos , Femenino , Persona de Mediana Edad , Endometrio/diagnóstico por imagen , Ultrasonografía Mamaria/métodos , Filariasis/diagnóstico por imagen , Endometrio/parasitología , Mama/diagnóstico por imagen , Mama/parasitología , Enfermedades de la Mama/parasitología , Diagnóstico DiferencialAsunto(s)
Endometrio/parasitología , Enterobiasis/complicaciones , Enterobius/patogenicidad , Trastornos de la Menstruación/parasitología , Adulto , Animales , Endometrio/fisiopatología , Enterobiasis/tratamiento farmacológico , Enterobius/efectos de los fármacos , Femenino , Humanos , Trastornos de la Menstruación/tratamiento farmacológico , Resultado del TratamientoRESUMEN
The present study was conducted in 300 female sex workers (FSWs) from Surat city in 2005-2006. Vaginal swabs, endocervical swabs and serum samples were collected from each of these FSWs. Vaginal samples were screened for bacterial vaginosis (BV), candidiasis and Trichomonas vaginalis . Endocervical swabs were screened for gonococcal infection. Serological tests for human immunodeficiency virus (HIV), hepatitis B surface antigen (HBsAg) and syphilis were performed. From a total of 300 FSWs, BV was detected in 40 (13.33%), trichomoniasis in six (2%), candidiasis in 31 (10.33%), HIV seropositivity in 35 (11.66%), HBsAg reactivity in 10 (3.33%) and rapid plasma regain (RPR) reactivity in 20 (6.66%) cases. RPR-positive serum samples were confirmed by the treponema pallidum hemaglutination test. Gonococcal infection was not found in any of the FSWs. Of the total of 35 HIV-positive patients, 20 patients had associated coinfection. Of the 35 HIV-seropositive FSWs, BV was detected in six (17.14%), candidiasis in six (17.14%), syphilis in five (14.28%) and HBsAg in two (5.71%). One (2.85%) HIV-positive FSW was positive for both candidiasis and syphilis.
Asunto(s)
Genitales Femeninos/microbiología , Genitales Femeninos/parasitología , Enfermedades de Transmisión Sexual/epidemiología , Candidiasis/epidemiología , Comorbilidad , Endometrio/microbiología , Endometrio/parasitología , Femenino , Gonorrea/epidemiología , Infecciones por VIH/epidemiología , Hepatitis B/epidemiología , Humanos , India/epidemiología , Suero/inmunología , Trabajo Sexual , Sífilis/epidemiología , Tricomoniasis/epidemiología , Vagina/microbiología , Vagina/parasitología , Vaginosis Bacteriana/epidemiologíaAsunto(s)
Endometrio/parasitología , Enterobiasis/diagnóstico , Adulto , Femenino , Humanos , Persona de Mediana EdadRESUMEN
Tritrichomonas foetus is an obligate parasite of the bovine urogenital tract producing infection associated with inflammatory changes, abortion, and infertility, Tritrichomonas mobilensis was isolated from squirrel monkey colon, and symptoms involve diarrheal complications. Both tritrichomonads produced hemagglutinins with the properties of sialic acid-specific lectins. Assays on the adherence of these protozoans to Chinese hamster ovary (CHO) cells and to bovine cervical and monkey colon mucus were performed to assess the function of the lectins in adhesion. Sialic acid at concentration as low as 2 mM inhibited the adhesion to CHO cells, less effectively to the mucus. Predigestion with Clostridium perfringens sialidase prevented the adhesion to both epithelial cells and the mucus. Inhibition of endogenous sialidases with 2,3-dehydro-2-deoxy-NeuAc increased the adhesion of T. mobilensis to CHO cells. Specific anti-T. foetus lectin (TFL) and anti-T. mobilensis lectin (TML) antibodies inhibited adhesion of the trichomonads to the epithelial cells and to the mucus. TFL histochemistry disclosed the presence of lectin ligands on keratinized vaginal epithelia, cervical mucosa, and mucin and on endometrial glands and their secretions. TML histochemistry showed reactivity with the luminal membranes of colonic glandular epithelium and less with the colonic mucin. Both lectins bound to the surface membrane of CHO cells. Anti-lectin antibodies showed granular cytoplasmic and strong membrane localization of the lectins in both tritrichomonads. Although the 2 tritrichomonads have different habitats, the results indicate that both these protozoa use lectins with sialic acid specificity for adhesion to mucosal surfaces.
Asunto(s)
Ácido N-Acetilneuramínico/farmacología , Tritrichomonas foetus/fisiología , Tritrichomonas/fisiología , Animales , Anticuerpos Monoclonales/inmunología , Anticuerpos Antiprotozoarios/inmunología , Células CHO , Bovinos , Adhesión Celular/efectos de los fármacos , Adhesión Celular/inmunología , Moco del Cuello Uterino/parasitología , Cuello del Útero/parasitología , Colon/parasitología , Cricetinae , Endometrio/parasitología , Femenino , Inmunoglobulina G/inmunología , Inmunohistoquímica , Mucosa Intestinal/parasitología , Lectinas/inmunología , Lectinas/fisiología , Ratones , Neuraminidasa/metabolismo , Neuraminidasa/farmacología , Conejos , Saimiri , Tritrichomonas/efectos de los fármacos , Tritrichomonas/inmunología , Tritrichomonas foetus/efectos de los fármacos , Tritrichomonas foetus/inmunología , Vagina/parasitologíaAsunto(s)
Endometrio/parasitología , Strongyloides stercoralis/aislamiento & purificación , Estrongiloidiasis/diagnóstico , Adulto , Animales , Antinematodos , Dilatación y Legrado Uterino , Endometrio/patología , Femenino , Estudios de Seguimiento , Humanos , Strongyloides stercoralis/efectos de los fármacos , Estrongiloidiasis/tratamiento farmacológico , Estrongiloidiasis/parasitología , Tiabendazol/uso terapéutico , Resultado del TratamientoRESUMEN
The human pinworm Enterobius vermicularis is normally found within the human gastrointestinal tract. Pregnant females migrate out of their host's anus at night to lay their eggs perianally. As a consequence of this nocturnal migration some worms find their way into adjacent orifices, most commonly the female genitourinary tract, producing irritative symptoms such as vulvovaginitis. A case of pinworm infestation of the uterus presented as postmenopausal bleeding.
Asunto(s)
Enterobiasis/complicaciones , Posmenopausia , Hemorragia Uterina/parasitología , Anciano , Endometrio/parasitología , Femenino , HumanosRESUMEN
PIP: This brief letter to the editor reports a case of filariasis diagnosed from a Papanicolaou smear (figure included). She was a 40-year-old woman who had been fitted with a Copper T 200 device 1 month before returning to complain of profuse bleeding during her first postinsertion menstrual cycle. The IUD was removed on Day 6 of cycle, and a routine smear showed 2 adult microfilariae in a background of erythrocytes. A figerprick blood smear did not confirm the diagnosis. The explanation for the endometrial smear finding is that the patient was an asymptomatic carrier and because of the rupture of endometrial capillaries during her menstrual bleeding, these organisms had escaped into the endometrial cavity.^ieng
Asunto(s)
Endometrio/parasitología , Filarioidea/aislamiento & purificación , Dispositivos Intrauterinos de Cobre , Microfilarias/aislamiento & purificación , Adulto , Endometrio/citología , Femenino , HumanosRESUMEN
Serologic testing for Toxoplasma antibodies (dye test, indirect fluorescent antibody test on serum, and complement-fixation test) was done in 96 women with habitual abortions and 61 women with sporadic abortions. In 61 of these women, endometrial biopsies were examined for content of T. gondii (animal inoculation procedures, immunofluorescence microscopy). Control subjects were 59 women with no spontaneous abortions. In seven patients (one control patient and six with habitual abortion), tachyzoites of T. gondii were observed in repeated biopsies from the endometrium and in menstrual blood by the immunofluorescence method. In no case, however, could T. gondii be isolated from the endometrium by inoculations. Moreover, the serologic results obtained among the patients with habitual abortion did not differ significantly from those obtained among the ones with sporadic abortion or among the control subjects. Five of the women with Toxoplasma-positive endometrium were serologically negative. Treatment of the Toxoplasma-positive women with antitoxoplasma drugs led to removal of parasites from the endometrium. T. gondii was not observed in the semen of the husbands.