RESUMEN
Some pathogen infections and immune system deficiencies have been linked to a few autoimmune diseases. However, the pathogenesis of most autoimmune diseases is unknown. An explanatory hypothesis for the pathogenesis of infection-initiated autoimmune diseases is provided. Virulent pathogen infections create extensive pathogen antigens that frequently require antibodies. These antibodies create extensive antigen-antibody immune complexes, which some immuno-compromised individuals will not adequately eliminate. This will cause inflammatory type III hypersensitivity symptoms, including protease releases that destroy epithelium, mesothelium and endothelium basement membranes, express new immunogenic antigens from previously sequestered basement membrane constituents, and ultimately induce new autoantibodies. This can continue after the infection ends, if the first wave of protease attacks on basement membranes induces new autoantibodies that cause new uncleared antigen-antibody immune complexes and type III hypersensitivity reactions. The secreted proteases and other enzymes will have preferred substrates and these proteases or other enzymes by themselves, or by their processed protein substrates, can express immunogenic antigens that induce new autoantibodies and initiate various autoimmune diseases. In summary, several autoimmune diseases can be initiated in immuno-compromised individuals during extensive pathogen infections, if these individuals have two immune problems: (a) slow or weak initial immune responses that result in a reliance on antibodies and (b) an inability to eliminate the resulting antigen-antibody immune complexes by phagocytosis. These two immune problems and the resulting immune system type III hypersensitivity reaction can explain the causation of several autoimmune diseases, including the most common and the rarest autoimmune diseases, both their differences and their similarities.
Asunto(s)
Enfermedades Autoinmunes/inmunología , Enfermedades Autoinmunes/patología , Huésped Inmunocomprometido/inmunología , Síndromes de Inmunodeficiencia/inmunología , Infecciones/inmunología , Complejo Antígeno-Anticuerpo/inmunología , Autoanticuerpos/inmunología , Humanos , Enfermedades del Complejo Inmune/inmunología , Enfermedades del Complejo Inmune/patologíaRESUMEN
Introduction: Multiple studies have revealed a strong relationship between the development of nephropathy and hepatitis B virus (HBV) infection. The underlying pathogenesis of hepatitis B-related glomerulonephritis (HBV-GN) involves immune complexes, which can be isolated from kidney tissues. Clearance of HBV antigenemia improves renal impairment and proteinuria in HBV-GN patients.Areas covered: In this review, we present our current understanding of the epidemiology, pathogenesis, pathology, diagnosis, and treatment of HBV-GN. We discuss the advantages and disadvantages of oral nucleoside/nucleotide analogs (NAs), and the main pharmaceutical treatment for hepatis B.Expert opinion: Currently, antiviral agents are the main HBV-GN therapeutic agents. Although no randomized controlled clinical trials have compared the efficacy of interferon (IFN) and NA, we suggest IFN treatment for pediatric patients (IFN-α in patients ≥1 year; pegIFN-α in patients ≥3 years) considering treatment duration and absence of resistance. Novel NAs have brought about promising treatment options involving high efficacy viral suppression and low resistance rates. NAs with a high barrier to resistance (e.g. entecavir) are recommended as first-line therapy of HBV-GN. Immunosuppression monotherapy, such as corticosteroids, is of little benefit and potentially harmful to HBV-GN patients due to the possibility of viral reactivation.
Asunto(s)
Glomerulonefritis/diagnóstico , Glomerulonefritis/tratamiento farmacológico , Hepatitis B Crónica/inmunología , Complejo Antígeno-Anticuerpo/inmunología , Antivirales/uso terapéutico , Glomerulonefritis/epidemiología , Glomerulonefritis/etiología , Hepatitis B Crónica/complicaciones , Hepatitis B Crónica/tratamiento farmacológico , Humanos , Enfermedades del Complejo Inmune/inmunología , Enfermedades del Complejo Inmune/patología , Enfermedades del Complejo Inmune/virología , Inmunosupresores/uso terapéutico , Interferón-alfa/uso terapéutico , Riñón/inmunología , Riñón/patología , Nucleósidos/efectos adversos , Nucleósidos/uso terapéutico , Nucleótidos/uso terapéuticoAsunto(s)
Enfermedad de Castleman/diagnóstico , Glomerulonefritis/diagnóstico , Enfermedades del Complejo Inmune/diagnóstico , Enfermedades de Inmunodeficiencia Primaria/diagnóstico , Proteínas Serina-Treonina Quinasas/genética , Adolescente , Síndrome de Bartter/diagnóstico , Enfermedad de Castleman/genética , Enfermedad de Castleman/inmunología , Enfermedad de Castleman/patología , Diagnóstico Diferencial , Femenino , Síndrome de Gitelman/diagnóstico , Glomerulonefritis/genética , Glomerulonefritis/inmunología , Glomerulonefritis/patología , Humanos , Enfermedades del Complejo Inmune/genética , Enfermedades del Complejo Inmune/inmunología , Enfermedades del Complejo Inmune/patología , Péptidos y Proteínas de Señalización Intracelular , Glomérulos Renales/patología , Ganglios Linfáticos/patología , Enfermedades de Inmunodeficiencia Primaria/genética , Enfermedades de Inmunodeficiencia Primaria/inmunología , Enfermedades de Inmunodeficiencia Primaria/patologíaRESUMEN
BACKGROUND: Chronic kidney disease (CKD) has typically a non-immune mediated origin in cats and immune-complex glomerulonephritis (ICGN) is scarcely described. Aims of this study were to characterize ICGN by light and electron microscopy and identify associations with clinico-pathological findings. In addition, comparisons between cats with ICGN and non immune-complex glomerulonephritis (non-ICGN) were performed. Renal samples examined between 2010 and 2019 were considered if both light and electron microscopy were performed. Signalment, feline immunodeficiency virus (FIV) and leukemia virus (FeLV) status, serum creatinine concentration, urine protein-to-creatinine (UPC) ratio, systolic blood pressure (SBP) and International Renal Interest Society (IRIS) stage were retrieved and used for comparisons. RESULTS: Sixty-eight client-owned cats were included. Thirty-seven cats (54.4%) had ICGN and 31 (45.6%) non-ICGN. Eighteen (48.6%) with ICGN had membranous glomerulonephropathy (MGN), 14 (37.8%) membranoproliferative glomerulonephritis (MPGN), and 5 (13.5%) mesangioproliferative glomerulonephritis (MeGN). Clinico-pathological data were not associated with any type of ICGN. Among cats with non-ICGN, 11 (35.5%) had end-stage CKD, 9 (29%) focal segmental glomerulosclerosis, 6 (19.4%) global and multifocal mesangiosclerosis, 2 (6.5%) glomerular atrophy, 2 (6.5%) renal dysplasia and 1 (3.1%) amyloidosis. Eight (25.8%) cats with non-ICGN had chronic interstitial nephritis (CIN) grade 1, 13 (41.9%) grade 2 and 10 (32.3%) grade 3; creatinine and UPC ratio increased with CIN grades (p = 0.001, p < 0.001). Cats with ICGN were more frequently FIV or FeLV-infected (OR:11.4; 95%CI:1.4-94.4; p = 0.024), had higher UPC ratio (OR:6.8; 95%CI:2.5-18.2; p < 0.001) and were younger (OR:0.9; 95%CI:0.7-1.0; p = 0.042) than cats with non-ICGN. CONCLUSIONS: MGN and MPGN were the most common morphological diagnoses of ICGN in cats. Unfortunately, none of the investigated findings differentiated ICGN morphological diagnoses. Serum creatinine concentration and UPC ratio were directly associated with grades of CIN (p = 0.001 and p < 0.001, respectively), confirming previous literature. More ICGN than non-ICGN was observed in cats with retroviral infections, younger cats and higher UPC ratio.
Asunto(s)
Enfermedades de los Gatos/patología , Glomerulonefritis/veterinaria , Enfermedades del Complejo Inmune/veterinaria , Animales , Gatos , Femenino , Glomerulonefritis/inmunología , Glomerulonefritis/patología , Enfermedades del Complejo Inmune/patología , Riñón/patología , Masculino , Estudios RetrospectivosRESUMEN
The atypical chemokine receptor 2 (ACKR2), also named D6, regulates local levels of inflammatory chemokines by internalization and degradation. To explore potential anti-inflammatory functions of ACKR2 in glomerulonephritis, we induced autologous nephrotoxic nephritis in C57/BL6 wild-type and Ackr2-deficient mice. Renal ACKR2 expression increased and localized to interstitial lymphatic endothelium during nephritis. At two weeks Ackr2-/-mice developed increased albuminuria and urea levels compared to wild-type mice. Histological analysis revealed increased structural damage in the glomerular and tubulointerstitial compartments within Ackr2-/- kidneys. This correlated with excessive renal leukocyte infiltration of CD4+ T cells and mononuclear phagocytes with increased numbers in the tubulointerstitium but not glomeruli in knockout mice. Expression of inflammatory mediators and especially markers of fibrotic tissue remodeling were increased along with higher levels of ACKR2 inflammatory chemokine ligands like CCL2 in nephritic Ackr2-/- kidneys. In vitro, Ackr2 deficiency in TNF-stimulated tubulointerstitial tissue but not glomeruli increased chemokine levels. These results are in line with ACKR2 expression in interstitial lymphatic endothelial cells, which also assures efflux of activated leukocytes into regional lymph nodes. Consistently, nephritic Ackr2-/- mice showed reduced adaptive cellular immune responses indicated by decreased regional T-cell activation. However, this did not prevent aggravated injury in the kidneys of Ackr2-/- mice with nephrotoxic nephritis due to simultaneously increased tubulointerstitial chemokine levels, leukocyte infiltration and fibrosis. Thus, ACKR2 is important in limiting renal inflammation and fibrotic remodeling in progressive nephrotoxic nephritis. Hence, ACKR2 may be a potential target for therapeutic interventions in immune complex glomerulonephritis.
Asunto(s)
Glomerulonefritis/prevención & control , Enfermedades del Complejo Inmune/prevención & control , Glomérulos Renales/metabolismo , Túbulos Renales/metabolismo , Receptores de Quimiocina/metabolismo , Inmunidad Adaptativa , Animales , Linfocitos T CD4-Positivos/inmunología , Linfocitos T CD4-Positivos/metabolismo , Células Cultivadas , Quimiotaxis de Leucocito , Modelos Animales de Enfermedad , Progresión de la Enfermedad , Fibrosis , Glomerulonefritis/inmunología , Glomerulonefritis/metabolismo , Glomerulonefritis/patología , Enfermedades del Complejo Inmune/inmunología , Enfermedades del Complejo Inmune/metabolismo , Enfermedades del Complejo Inmune/patología , Mediadores de Inflamación/metabolismo , Glomérulos Renales/inmunología , Glomérulos Renales/patología , Túbulos Renales/inmunología , Túbulos Renales/patología , Activación de Linfocitos , Masculino , Ratones Endogámicos C57BL , Ratones Noqueados , Sistema Mononuclear Fagocítico/inmunología , Sistema Mononuclear Fagocítico/metabolismo , Receptores de Quimiocina/deficiencia , Receptores de Quimiocina/genética , Transducción de SeñalRESUMEN
Human immunodeficiency virus (HIV) infection continues to be a leading cause of morbidity and mortality. HIV-infected individuals are now surviving for a relatively longer period and this is because of easy accessibility to antiretroviral therapy these days. As a result, chronic disease-related complications are now being recognized more often. Kidney disease in HIV-infected children can vary from glomerular to tubular-interstitial involvement. We searched the database to identify various kidney diseases seen in HIV-infected children. We describe the epidemiology, pathogenesis, pathology, clinical and laboratory manifestations, management and outcome of commonly seen kidney disease in HIV-infected children. We also provide a brief overview of toxicity of antiretroviral drugs seen in HIV-infected children. Kidney involvement in HIV-infected children may arise because of HIV infection per se, opportunistic infections, immune mediated injury and drug toxicity. HIV-associated nephropathy is perhaps the most common and most severe form of kidney disease. Proteinuria may be a cost-effective screening test in the long-term management of HIV-infected children, however, there are no definite recommendations for the same. Other important renal diseases are HIV immune complex kidney disease, thrombotic microangiopathy, interstitial nephritis and vasculitis.
Asunto(s)
Nefropatía Asociada a SIDA , Antirretrovirales/efectos adversos , Infecciones por VIH/complicaciones , Enfermedades Renales/complicaciones , Riñón/efectos de los fármacos , Riñón/patología , Proteinuria/diagnóstico , Nefropatía Asociada a SIDA/epidemiología , Nefropatía Asociada a SIDA/etiología , Nefropatía Asociada a SIDA/patología , Nefropatía Asociada a SIDA/terapia , Animales , Antirretrovirales/uso terapéutico , Antirretrovirales/toxicidad , Niño , Humanos , Enfermedades del Complejo Inmune/epidemiología , Enfermedades del Complejo Inmune/etiología , Enfermedades del Complejo Inmune/patología , Enfermedades del Complejo Inmune/terapia , Nefritis/etiología , Nefritis/patología , Nefritis/terapia , Proteinuria/patología , Microangiopatías Trombóticas/etiología , Microangiopatías Trombóticas/patología , Microangiopatías Trombóticas/terapia , Vasculitis/etiología , Vasculitis/patología , Vasculitis/terapiaRESUMEN
Immune complex deposition in kidney allografts can include both recurrent and de novo processes. Recurrent glomerulonephritis is a well-recognized phenomenon and has been shown to be a common cause of allograft failure. De novo immune complex-mediated disease remains relatively poorly characterized, likely owing to the less frequent use of immunofluorescence and electron microscopy in the transplant setting. We performed a retrospective review of kidney allograft biopsies showing glomerular immune complex deposition. Cases with de novo deposits were identified and further organized into two groups depending on whether the immune complex deposition could be clinically and/or histologically classified. Thirty-two patients with de novo immune complex deposition were identified over a 7-year period. A broad range of immune complex-mediated injuries were observed, the majority (63%) of which could be readily classified either clinically or histologically. These included cases of membranous glomerulonephropathy, IgA nephropathy, infection-related glomerulonephritis and glomerulonephritis related to an underlying autoimmune process. A smaller subset of patients (37%) demonstrated immune complex deposition that was difficult to histologically or clinically classify. These patients typically showed mild mesangial immune complex deposition with co-dominant IgG and IgM staining by immunofluorescence microscopy. The presence of concurrent antibody-mediated rejection and donor-specific antibody positivity was significantly higher in the unclassifiable group. The significance of these deposits and their possible relationship to allograft rejection deserves further investigation.
Asunto(s)
Glomerulonefritis/inmunología , Enfermedades del Complejo Inmune/patología , Trasplante de Riñón , Adulto , Anciano , Aloinjertos , Complejo Antígeno-Anticuerpo/inmunología , Femenino , Humanos , Enfermedades del Complejo Inmune/inmunología , Masculino , Persona de Mediana Edad , Estudios RetrospectivosRESUMEN
Ubiquitin C-terminal hydrolase L1 (UCH-L1) is a major deubiquitinating enzyme of the nervous system and associated with the development of neurodegenerative diseases. We have previously shown that UCH-L1 is found in tubular and parietal cells of the kidney and is expressed de novo in injured podocytes. Since the role of UCH-L1 in the kidney is unknown we generated mice with a constitutive UCH-L1-deficiency to determine its role in renal health and disease. UCH-L1-deficient mice developed proteinuria, without gross changes in glomerular morphology. Tubular cells, endothelial cells, and podocytes showed signs of stress with an accumulation of oxidative-modified and polyubiquitinated proteins. Mechanistically, abnormal protein accumulation resulted from an altered proteasome abundance leading to decreased proteasomal activity, a finding exaggerated after induction of anti-podocyte nephritis. UCH-L1-deficient mice exhibited an exacerbated course of disease with increased tubulointerstitial and glomerular damage, acute renal failure, and death, the latter most likely a result of general neurologic impairment. Thus, UCH-L1 is required for regulated protein degradation in the kidney by controlling proteasome abundance. Altered proteasome abundance renders renal cells, particularly podocytes and endothelial cells, susceptible to injury.
Asunto(s)
Glomerulonefritis/enzimología , Enfermedades del Complejo Inmune/enzimología , Podocitos/enzimología , Complejo de la Endopetidasa Proteasomal/metabolismo , Ubiquitina Tiolesterasa/metabolismo , Ubiquitina/metabolismo , Animales , Células Cultivadas , Modelos Animales de Enfermedad , Glomerulonefritis/genética , Glomerulonefritis/inmunología , Glomerulonefritis/patología , Hipotensión/enzimología , Hipotensión/genética , Enfermedades del Complejo Inmune/genética , Enfermedades del Complejo Inmune/inmunología , Enfermedades del Complejo Inmune/patología , Péptidos y Proteínas de Señalización Intracelular/deficiencia , Péptidos y Proteínas de Señalización Intracelular/genética , Proteínas de la Membrana/deficiencia , Proteínas de la Membrana/genética , Ratones Noqueados , Oxidación-Reducción , Podocitos/inmunología , Podocitos/patología , Proteinuria/enzimología , Proteinuria/genética , Proteolisis , Ubiquitina Tiolesterasa/deficiencia , Ubiquitina Tiolesterasa/genética , UbiquitinaciónRESUMEN
A series of 78 cases of glomerulonephritis (GN), in which renal biopsy revealed changes of GN associated with crescent formation, were reviewed. Renal pathology findings were correlated with clinical features including patient's age, renal function, and serologic findings. In most of the cases (71.8%), the crescents were due to immune complex-mediated GN. This was followed by pauci-immune GN (20.5%) and anti-glomerular basement membrane antibody (GBM) GN (7.7%). The percentage of glomeruli with crescents was the highest in cases of anti-GBM disease (mean of 93.3%), followed by pauci-immune GBM (mean of 48.2%) and immune complex GN (30.9%). In cases with the pauci- immune GN, there were additional features of glomerular injury including fibrinoid necrosis, disruption of the GBM, and rupture of Bowman's capsule. These changes were generally more pronounced in a subset of pauci-immune GN associated with serum elevation of antineutrophil cytoplasmic antibody (c-ANCA). In biopsies from patient with immune complex disease, systemic lupus erythematosus was the most common cause of crescentic GN.
Asunto(s)
Glomerulonefritis/patología , Enfermedades del Complejo Inmune/patología , Glomérulos Renales/patología , Centros de Atención Terciaria , Adulto , Anciano , Enfermedad por Anticuerpos Antimembrana Basal Glomerular/inmunología , Enfermedad por Anticuerpos Antimembrana Basal Glomerular/patología , Enfermedad por Anticuerpos Antimembrana Basal Glomerular/fisiopatología , Anticuerpos Anticitoplasma de Neutrófilos/análisis , Complejo Antígeno-Anticuerpo/análisis , Autoanticuerpos/análisis , Biomarcadores/análisis , Biopsia , Femenino , Glomerulonefritis/inmunología , Glomerulonefritis/fisiopatología , Humanos , Enfermedades del Complejo Inmune/inmunología , Enfermedades del Complejo Inmune/fisiopatología , Glomérulos Renales/inmunología , Glomérulos Renales/fisiopatología , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Arabia Saudita , Adulto JovenAsunto(s)
Infecciones por VIH/complicaciones , Enfermedades del Complejo Inmune/patología , Enfermedades Renales/patología , Riñón/patología , Complemento C1q/metabolismo , Complemento C3/metabolismo , Humanos , Enfermedades del Complejo Inmune/etiología , Enfermedades del Complejo Inmune/metabolismo , Inmunoglobulina A/metabolismo , Inmunoglobulina G/metabolismo , Inmunoglobulina M/metabolismo , Riñón/metabolismo , Riñón/ultraestructura , Enfermedades Renales/etiología , Enfermedades Renales/metabolismo , Microscopía , Microscopía Electrónica , Microscopía FluorescenteRESUMEN
BACKGROUND: To clarify the clinical manifestations of pediatric complement component C3 glomerulonephritis (C3GN), we retrospectively evaluated differences in the clinicopathological findings and prognosis between C3GN and immune-complex-mediated membranoproliferative glomerulonephritis (IC-MPGN). METHODS: Thirty-seven patients diagnosed with "idiopathic MPGN" were enrolled in this retrospective study. The patients were divided into two groups, with Group 1 consisting of 19 patients diagnosed with IC-MPGN and Group 2 consisting of 18 patients diagnosed with C3GN. The clinical findings and the prognosis were investigated for both groups. RESULTS: Thirteen patients in Group 2 were identified by mandatory annual school screening for urinary abnormalities. The incidence of macro-hematuria and the frequency of low serum C4 values were lower in Group 2 patients than in Group 1 patients. At the time of the second renal biopsy, urinary protein excretion, incidence of hematuria, frequency of low serum C3 values, and scores for mesangial proliferation, glomerular sclerosis, and interstitial fibrosis were higher in Group 2 patients than in Group 1 patients. At the most recent follow-up examination, the number of patients categorized as non-responding or with end-stage renal disease was higher in Group 2 patients than in Group 1 patients. CONCLUSIONS: Our results suggest that the treatment response and prognosis of patients with C3GN are worse than those of patients with IC-mediated MPGN. Therefore, in the clinical context regarding treatment options and prognosis, it may be useful to classify idiopathic MPGN as C3GN or IC-MPGN. In addition, long-term follow-up of C3GN is necessary.
Asunto(s)
Complemento C3 , Glomerulonefritis Membranoproliferativa/patología , Enfermedades del Complejo Inmune/patología , Niño , Femenino , Glomerulonefritis Membranoproliferativa/epidemiología , Glomerulonefritis Membranoproliferativa/inmunología , Humanos , Enfermedades del Complejo Inmune/epidemiología , Enfermedades del Complejo Inmune/inmunología , Inmunohistoquímica , Incidencia , Masculino , Estudios RetrospectivosRESUMEN
We have observed a predominantly mesangial non-immunoglobulin A immune complex mesangial glomerulopathy (MG) in renal transplants with mesangial deposits by immunofluorescence and electron microscopy. Clinicopathological features of 28 patients with MG were analyzed and compared with 28 transplant controls, matched for age, sex, ethnicity, donor type, estimated glomerular filtration rate, and interval from transplant to biopsy. Indications for biopsy in the MG group were allograft dysfunction in 64%, allograft dysfunction/proteinuria in 29%, and proteinuria in 7%. Biopsy indications in controls were allograft dysfunction (61%), allograft dysfunction/proteinuria (18%), proteinuria (14%), and delayed graft function (7%). Most MG cases had mild mesangial hypercellularity with endocapillary proliferation in 2 and crescents in 2 without fibrinoid necrosis. Immunoglobulin M-dominant deposits were present in 83%, and immunoglobulin G was dominant in 17% with mesangial deposits in 93% of cases by electron microscopy. Compared with controls, MG had higher Banff interstitial inflammation score (i) (P = .036) and was associated with concurrent acute T-cell-mediated rejection (P = .023), but not with acute or chronic antibody-mediated rejection. MG patients and controls had similar prevalence of polyomavirus nephropathy and Epstein-Barr virus infection. At follow-up, most MG patients had stable estimated glomerular filtration rate with no or stable proteinuria. Disease-specific graft survival was not different in MG versus controls. We conclude that, in view of the apparent self-limited nature of this lesion, additional treatment may not be required in these patients. Awareness of this lesion may thus spare patients unwarranted further intervention.
Asunto(s)
Glomerulonefritis/patología , Enfermedades del Complejo Inmune/patología , Trasplante de Riñón/efectos adversos , Adolescente , Adulto , Aloinjertos , Niño , Femenino , Técnica del Anticuerpo Fluorescente , Mesangio Glomerular/patología , Glomerulonefritis/epidemiología , Glomerulonefritis/etiología , Humanos , Enfermedades del Complejo Inmune/epidemiología , Enfermedades del Complejo Inmune/etiología , Masculino , Persona de Mediana Edad , Prevalencia , Adulto JovenRESUMEN
In this issue of Blood, Gros et al report that glycoprotein VI (GPVI) promotes the proinflammatory role of platelets by increasing neutrophil secretion and toxicity while at the same time repairing the vascular damage inflicted by neutrophil activation, thereby maintaining vascular integrity. Significantly, this effect is independent of hemostasis.
Asunto(s)
Plaquetas/metabolismo , Enfermedades del Complejo Inmune/patología , Inflamación/patología , Neutrófilos/patología , Glicoproteínas de Membrana Plaquetaria/metabolismo , AnimalesRESUMEN
Platelets protect vascular integrity during inflammation. Recent evidence suggests that this action is independent of thrombus formation and requires the engagement of glycoprotein VI (GPVI), but it remains unclear how platelets prevent inflammatory bleeding. We investigated whether platelets and GPVI act primarily by preventing detrimental effects of neutrophils using models of immune complex (IC)-mediated inflammation in mice immunodepleted in platelets and/or neutrophils or deficient in GPVI. Depletion of neutrophils prevented bleeding in thrombocytopenic and GPVI(-/-) mice during IC-mediated dermatitis. GPVI deficiency did not modify neutrophil recruitment, which was reduced by thrombocytopenia. Neutrophil cytotoxic activities were reduced in thrombocytopenic and GPVI(-/-) mice during IC-mediated inflammation. Intravital microscopy revealed that in this setting, intravascular binding sites for platelets were exposed by neutrophils, and GPVI supported the recruitment of individual platelets to these spots. Furthermore, the platelet secretory response accompanying IC-mediated inflammation was partly mediated by GPVI, and blocking of GPVI signaling impaired the vasculoprotective action of platelets. Together, our results show that GPVI plays a dual role in inflammation by enhancing neutrophil-damaging activities while supporting the activation and hemostatic adhesion of single platelets to neutrophil-induced vascular breaches.
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Plaquetas/metabolismo , Enfermedades del Complejo Inmune/patología , Inflamación/patología , Neutrófilos/patología , Glicoproteínas de Membrana Plaquetaria/metabolismo , Animales , Modelos Animales de Enfermedad , Enfermedades del Complejo Inmune/complicaciones , Mediadores de Inflamación/metabolismo , Ratones , Ratones Endogámicos C57BL , Ratones NoqueadosRESUMEN
There were performed the studies of genotoxic stress and the ways of immunocompetent cells death (apoptosis and necrosis) in the modeling of immune system damage by immunization of CBA mice with the bovine serum albumin. Immunofluorescence studies of immunized mice were established the fixation of immune complexes in liver tissue, spleen, kidney and the aorta. Histological studies of these organs showed vascular system affection and, to a lesser extent, parenchyma. It has been shown that DNA comets index increases in 1,4 time in the lymph node cells and in 1,5 time in the thymus cells in the presence of BSA immunization. We also observed an increase in the number of cells with maximum damage DNA thymus preparations (3.4 fold) and lymph nodes (3.3-fold), respectively, indicating strong genotoxic stress. There were shown the reduce of live ICC number and their death increase, including the pro-inflammatory and immunogenic necrotic way. In that way, data which were obtained on the experimental model is evidenced that generalized immunecomplex pathologic process leads to DNA damage and ICC death both central and peripheral organs of the immune system. ICC genotoxic stress and their death amplification by the necrotic way may play a significant role in the immunecomplex deseases development. These factors of peripheral blood lymphocytes can serve as a prospective test system for assessing the severity of autoimmune and immune complex diseases and their treatment effectiveness.
Asunto(s)
Complejo Antígeno-Anticuerpo/sangre , Daño del ADN/inmunología , Enfermedades del Complejo Inmune/patología , Necrosis/patología , Timo/patología , Animales , Aorta/inmunología , Aorta/metabolismo , Aorta/patología , Apoptosis/inmunología , Bovinos , Ensayo Cometa , Modelos Animales de Enfermedad , Femenino , Enfermedades del Complejo Inmune/sangre , Enfermedades del Complejo Inmune/inducido químicamente , Enfermedades del Complejo Inmune/inmunología , Inmunización , Riñón/inmunología , Riñón/metabolismo , Riñón/patología , Ganglios Linfáticos/inmunología , Ganglios Linfáticos/metabolismo , Ganglios Linfáticos/patología , Ratones , Ratones Endogámicos CBA , Necrosis/inducido químicamente , Necrosis/inmunología , Necrosis/metabolismo , Albúmina Sérica Bovina/administración & dosificación , Bazo/inmunología , Bazo/metabolismo , Bazo/patología , Timo/inmunología , Timo/metabolismoRESUMEN
AIM: Interactions between the co-stimulatory molecule programmed death 1 (PD-1) and its ligands, PD-L1 and PD-L2, constrain T-cell responses and help maintain peripheral tolerance. Glomerulonephritis can result from a variety of antigens, both self and foreign, and from humoural and cellular effector responses. These studies aimed to define the role of PD1 and its ligands in circulating immune complex glomerulonephritis induced by immunity to a foreign antigen. METHODS: Immune complex glomerulonephritis was initiated by injecting BALB/c mice with horse spleen apoferritin intraperitoneally daily for 14 days. Inhibitory anti-mouse PD-1, anti-PD-L1 or anti-PD-L2 antibodies were administered every other day. Renal disease and immune responses were studied. RESULTS: Daily injection of horse spleen apoferritin-induced proliferative immune complex glomerulonephritis in control antibody-treated mice, but inhibiting PD-1 did not augment renal injury. Specifically, blocking PD-1 did not increase serum antigen-specific antibodies or increase glomerular immunoglobulin G deposition, the hallmark of injury in this model. Furthermore, C3 deposition was unaffected and glomerular macrophages were reduced after anti-PD-1 antibodies. However, anti-PD-1 administration did increase splenocyte proliferation and cytokine production including interferon-γ, interleukin (IL)-4, and IL-17, but not IL-10. Neutralizing either PD-L1 or PD-L2 alone did not result in major alterations in renal injury. CONCLUSION: The endogenous PD-1/PD-L pathway does not limit acute experimental foreign antigen-induced circulating immune complex glomerulonephritis.
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Antígenos , Apoferritinas , Antígeno B7-H1/inmunología , Glomerulonefritis/inmunología , Enfermedades del Complejo Inmune/inmunología , Glomérulos Renales/inmunología , Proteína 2 Ligando de Muerte Celular Programada 1/inmunología , Receptor de Muerte Celular Programada 1/inmunología , Animales , Anticuerpos/administración & dosificación , Antígeno B7-H1/antagonistas & inhibidores , Antígeno B7-H1/metabolismo , Células Cultivadas , Citocinas/inmunología , Citocinas/metabolismo , Modelos Animales de Enfermedad , Glomerulonefritis/inducido químicamente , Glomerulonefritis/metabolismo , Glomerulonefritis/patología , Enfermedades del Complejo Inmune/inducido químicamente , Enfermedades del Complejo Inmune/metabolismo , Enfermedades del Complejo Inmune/patología , Inmunidad Humoral , Inmunoglobulina G/inmunología , Inmunoglobulina G/metabolismo , Mediadores de Inflamación/inmunología , Mediadores de Inflamación/metabolismo , Glomérulos Renales/efectos de los fármacos , Glomérulos Renales/metabolismo , Glomérulos Renales/patología , Macrófagos/inmunología , Macrófagos/metabolismo , Masculino , Ratones Endogámicos BALB C , Proteína 2 Ligando de Muerte Celular Programada 1/antagonistas & inhibidores , Proteína 2 Ligando de Muerte Celular Programada 1/metabolismo , Receptor de Muerte Celular Programada 1/antagonistas & inhibidores , Receptor de Muerte Celular Programada 1/metabolismo , Transducción de Señal , Bazo/inmunología , Bazo/metabolismo , Factores de TiempoRESUMEN
We systematically examined by immune histology the lungs of some widely used mouse models of asthma. These models include sensitization by multiple intraperitoneal injections of soluble ovalbumin (OVA) or of OVA with alum, followed by three intranasal or aerosol challenges 3 days apart. Within 24 h after a single challenge there is fibrinoid necrosis of arterial walls with deposition of immunoglobulin (Ig) and OVA and infiltration of eosinophilic polymorphonuclear cells that lasts for about 3 days followed by peribronchial B-cell infiltration and slight reversible goblet cell hypertrophy (GCHT). After two challenges, severe eosinophilic vasculitis is present at 6 h, increases by 72 h, and then declines; B-cell proliferation and significant GCHT and hyperplasia (GCHTH) and bronchial smooth muscle hypertrophy recur more prominently. After three challenges, there is significantly increased induced bronchus-associated lymphoid tissue (iBALT) formation, GCHTH, and smooth muscle hypertrophy. Elevated levels of Th2 cytokines, IL-4, IL-5, and IL-13, are present in bronchial lavage fluids. Sensitized mice have precipitating antibody and positive Arthus skin reactions but also develop significant levels of IgE antibody to OVA but only 1 week after challenge. We conclude that the asthma like lung lesions induced in these models is preceded by immune complex-mediated eosinophilic vasculitis and iBALT formation. There are elevations of Th2 cytokines that most likely produce bronchial lesions that resemble human asthma. However, it is unlikely that mast cell-activated atopic mechanisms are responsible as we found only a few presumed mast cells by toluidine blue and metachromatic staining limited to the most proximal part of the main stem bronchus, and none in the remaining main stem bronchus or in the lung periphery.
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Asma/patología , Bronquios/patología , Enfermedades del Complejo Inmune/patología , Tejido Linfoide/patología , Vasculitis/patología , Animales , Asma/inmunología , Bronquios/química , Bronquios/inmunología , Citocinas/análisis , Modelos Animales de Enfermedad , Eosinofilia , Femenino , Enfermedades del Complejo Inmune/inmunología , Inmunohistoquímica , Tejido Linfoide/química , Tejido Linfoide/inmunología , Ratones , Ratones Endogámicos BALB C , Mucosa Respiratoria/química , Mucosa Respiratoria/inmunología , Mucosa Respiratoria/patología , Vasculitis/inmunologíaRESUMEN
Small vessel vasculitis is a life-threatening condition and patients typically present with renal and pulmonary injury. Disease pathogenesis is associated with neutrophil accumulation, activation, and oxidative damage, the latter being driven in large part by myeloperoxidase (MPO), which generates hypochlorous acid among other oxidants. MPO has been associated with vasculitis, disseminated vascular inflammation typically involving pulmonary and renal microvasculature and often resulting in critical consequences. MPO contributes to vascular injury by 1) catabolizing nitric oxide, impairing vasomotor function; 2) causing oxidative damage to lipoproteins and endothelial cells, leading to atherosclerosis; and 3) stimulating formation of neutrophil extracellular traps, resulting in vessel occlusion and thrombosis. Here we report a selective 2-thiouracil mechanism-based MPO inhibitor (PF-1355 [2-(6-(2,5-dimethoxyphenyl)-4-oxo-2-thioxo-3,4-dihydropyrimidin-1(2H)-yl)acetamide) and demonstrate that MPO is a critical mediator of vasculitis in mouse disease models. A pharmacokinetic/pharmacodynamic response model of PF-1355 exposure in relation with MPO activity was derived from mouse peritonitis. The contribution of MPO activity to vasculitis was then examined in an immune complex model of pulmonary disease. Oral administration of PF-1355 reduced plasma MPO activity, vascular edema, neutrophil recruitment, and elevated circulating cytokines. In a model of anti-glomerular basement membrane disease, formerly known as Goodpasture disease, albuminuria and chronic renal dysfunction were completely suppressed by PF-1355 treatment. This study shows that MPO activity is critical in driving immune complex vasculitis and provides confidence in testing the hypothesis that MPO inhibition will provide benefit in treating human vasculitic diseases.
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Acetamidas/farmacología , Inhibidores Enzimáticos/farmacología , Membrana Basal Glomerular/efectos de los fármacos , Glomerulonefritis/prevención & control , Enfermedades del Complejo Inmune/prevención & control , Peroxidasa/antagonistas & inhibidores , Pirimidinas/farmacología , Pirimidinonas/farmacología , Vasculitis/prevención & control , Animales , Membrana Basal Glomerular/patología , Glomerulonefritis/enzimología , Glomerulonefritis/inmunología , Glomerulonefritis/patología , Humanos , Enfermedades del Complejo Inmune/enzimología , Enfermedades del Complejo Inmune/inmunología , Enfermedades del Complejo Inmune/patología , Pulmón/irrigación sanguínea , Pulmón/efectos de los fármacos , Pulmón/inmunología , Ratones , Infiltración Neutrófila/efectos de los fármacos , Transducción de Señal/efectos de los fármacos , Vasculitis/enzimología , Vasculitis/inmunología , Vasculitis/patologíaRESUMEN
BACKGROUND: Traditionally, antineutrophil cytoplasmic autoantibody-associated vasculitis (AAV) is histologically characterized by pauci-immune glomerulonephritis. However, more and more literature has reported immune complex (IC) deposits to be found in renal specimen from patients with AAV. The role that these IC deposits play in the development of AAV, as well as their clinical and pathological significance, is worthy of studying. OBJECTIVES: The objective of this study was to analyze the clinical and pathological characteristics of Chinese patients with AAV having renal IC deposition. METHODS: A retrospective study was performed on 34 patients with AAV in Shanghai Ruijin Hospital with renal IC deposition. Clinical and pathological data were collected and studied and compared with other 76 AAV patients having classic pauci-immune glomerulonephritis. RESULTS: Thirty-four patients were enrolled in this study, with a mean age of 56.4 ± 16.4 years and a male-female ratio of 1:1.3 (19/15). Twenty-seven patients (79.4%) had impaired renal function, with an average serum creatinine of 4.4 ± 3.2 mg/dL. C3 (82.4%) and immunoglobulin M (50%) were the most common IC deposits observed in the kidneys. During the follow-up (median, 39 months), 6 patients (17.7%) died, and 11 (32.4%) finally progressed to end-stage renal disease despite immunosuppressive therapy. Compared with patients having classic pauci-immune glomerulonephritis, patients with renal IC deposits had similar clinical and laboratory features except for more proteinuria (2374 ± 2221 vs 1444 ± 1956 mg/24 h, P = 0.002), a higher prevalence of nephrotic syndrome (30.3% vs 9.6%, P = 0.007) and hypocomplementemia (86.8 ± 33.1 vs 110 ± 45.5 mg/dL, P = 0.029), and also a higher risk for progressing to end-stage renal disease (32.4% vs 13.1%, P = 0.018). CONCLUSIONS: Patients with AAV with renal IC deposition might have a worse renal prognosis than those having classic pauci-immune glomerulonephritis.
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Vasculitis Asociada a Anticuerpos Citoplasmáticos Antineutrófilos/diagnóstico , Vasculitis Asociada a Anticuerpos Citoplasmáticos Antineutrófilos/patología , Complejo Antígeno-Anticuerpo/metabolismo , Enfermedades del Complejo Inmune/diagnóstico , Enfermedades del Complejo Inmune/patología , Riñón/metabolismo , Riñón/patología , Corticoesteroides/uso terapéutico , Adulto , Anciano , Vasculitis Asociada a Anticuerpos Citoplasmáticos Antineutrófilos/mortalidad , China , Comorbilidad , Progresión de la Enfermedad , Femenino , Estudios de Seguimiento , Glomerulonefritis/diagnóstico , Glomerulonefritis/mortalidad , Glomerulonefritis/patología , Humanos , Enfermedades del Complejo Inmune/mortalidad , Estimación de Kaplan-Meier , Masculino , Persona de Mediana Edad , Síndrome Nefrótico , Prevalencia , Pronóstico , Estudios Retrospectivos , Resultado del TratamientoRESUMEN
Most hypersensitivity reactions to insect stings are immediate, ranging from transient local reactions of little medical consequence to fatal anaphylaxis. Rarely, some patients have delayed reactions after a period of apparent normality which manifest as systemic features which can be life-threatening. A 3-year-old boy was attacked by a swarm of bees, estimated to be about 200 in number. There was an immediate cutaneous reaction which was treated at a local hospital. After 9 days, he presented with oliguria, dark-coloured urine, pedal oedema, hypertension and acute kidney injury (AKI). He was managed conservatively with fluid restriction, control of blood pressure and peritoneal dialysis, and renal function returned to normal gradually over the following 9 days. The delayed-onset AKI and other laboratory abnormalities suggested a immune-mediated type III hypersensitivity reaction leading to renal insufficiency. After improvement of initial hypersensitivity reactions, patients with bee stings should be followed up in order to detect any late-onset complications which might be life-threatening.