RESUMEN
To compare free-water corrected diffusion tensor imaging (DTI) measures in the normal-appearing periependymal area between AQP4-IgG-seropositive NMOSD and multiple sclerosis (MS) to investigate occult pathophysiology. This prospective study included 44 patients (mean age, 39.52 ± 11.90 years; 14 men) with AQP4-IgG-seropositive NMOSD (n = 20) and MS (n = 24) who underwent DTI between April 2014 and April 2020. Based on free-water corrected DTI measures obtained from normal-appearing periependymal voxels of (1) lateral ventricles and (2) the 3rd and 4th ventricles as dependent variables, MANCOVA was conducted to compare the two groups, using clinical variables as covariates. A significant difference was found between AQP4-IgG-seropositive NMOSD and MS in the 3rd and 4th periependymal voxels (λ = 0.462, P = 0.001). Fractional anisotropy, axial diffusivity was significantly decreased and radial diffusivity was increased in AQP4-IgG-seropositive NMOSD in post-hoc analysis, compared with MS (F = 27.616, P < 0.001, F = 7.336, P = 0.011, and F = 5.800, P = 0.022, respectively). Free-water corrected DTI measures differ in the periependymal area surrounding the diencephalon and brain stem/cerebellum between MS and NMOSD, which may suggest occult white matter injury in areas with distribution of AQP-4 in NMOSD.
Asunto(s)
Acuaporina 4/inmunología , Autoanticuerpos/sangre , Epéndimo/diagnóstico por imagen , Inmunoglobulina G/sangre , Neuromielitis Óptica/diagnóstico por imagen , Adulto , Autoanticuerpos/inmunología , Encéfalo/diagnóstico por imagen , Imagen de Difusión Tensora , Epéndimo/anomalías , Epéndimo/inmunología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Neuromielitis Óptica/sangre , Neuromielitis Óptica/inmunología , Estudios ProspectivosRESUMEN
The administration of microbial neuraminidase into the brain ventricular cavities of rodents represents a model of acute aseptic neuroinflammation. Ependymal cell death and hydrocephalus are unique features of this model. Here we demonstrate that activated microglia participates in ependymal cell death. Co-cultures of pure microglia with ependymal cells (both obtained from rats) were performed, and neuraminidase or lipopolysaccharide were used to activate microglia. Ependymal cell viability was unaltered in the absence of microglia or inflammatory stimulus (neuraminidase or lipopolysaccharide). The constitutive expression by ependymal cells of receptors for cytokines released by activated microglia, such as IL-1ß, was demonstrated by qPCR. Besides, neuraminidase induced the overexpression of both receptors in ventricular wall explants. Finally, ependymal viability was evaluated in the presence of functional blocking antibodies against IL-1ß and TNFα. In the co-culture setting, an IL-1ß blocking antibody prevented ependymal cell death, while TNFα antibody did not. These results suggest that activated microglia are involved in the ependymal damage that occurs after the administration of neuraminidase in the ventricular cavities, and points to IL-1ß as possible mediator of such effect. The relevance of these results lies in the fact that brain infections caused by neuraminidase-bearing pathogens are frequently associated to ependymal death and hydrocephalus.
Asunto(s)
Muerte Celular/inmunología , Epéndimo/inmunología , Microglía/inmunología , Neuraminidasa/farmacología , Animales , Células Cultivadas , Epéndimo/citología , Interleucina-1beta , Lipopolisacáridos/farmacología , Masculino , Ratas , Ratas WistarRESUMEN
A previously unreported population of foam cells (foamy macrophages) accumulates in the invasive fibrotic meninges during gap regeneration of transected adult Axolotl spinal cord (salamander Ambystoma mexicanum) and may act beneficially. Multinucleated giant cells (MNGCs) also occurred in the fibrotic meninges. Actin-label localization and transmission electron microscopy showed characteristic foam cell and MNGC podosome and ruffled border-containing sealing ring structures involved in substratum attachment, with characteristic intermediate filament accumulations surrounding nuclei. These cells co-localized with regenerating cord ependymal cell (ependymoglial) outgrowth. Phase contrast-bright droplets labeled with Oil Red O, DiI, and DyRect polar lipid live cell label showed accumulated foamy macrophages to be heavily lipid-laden, while reactive ependymoglia contained smaller lipid droplets. Both cell types contained both neutral and polar lipids in lipid droplets. Foamy macrophages and ependymoglia expressed the lipid scavenger receptor CD36 (fatty acid translocase) and the co-transporter toll-like receptor-4 (TLR4). Competitive inhibitor treatment using the modified fatty acid Sulfo-N-succinimidyl Oleate verified the role of the lipid scavenger receptor CD36 in lipid uptake studies in vitro. Fluoromyelin staining showed both cell types took up myelin fragments in situ during the regeneration process. Foam cells took up DiI-Ox-LDL and DiI-myelin fragments in vitro while ependymoglia took up only DiI-myelin in vitro. Both cell types expressed the cysteine proteinase cathepsin K, with foam cells sequestering cathepsin K within the sealing ring adjacent to the culture substratum. The two cell types act as sinks for Ox-LDL and myelin fragments within the lesion site, with foamy macrophages showing more Ox-LDL uptake activity. Cathepsin K activity and cellular localization suggested that foamy macrophages digest ECM within reactive meninges, while ependymal cells act from within the spinal cord tissue during outgrowth into the lesion site, acting in complementary fashion. Small MNGCs also expressed lipid transporters and showed cathepsin K activity. Comparison of 3H-glucosamine uptake in ependymal cells and foam cells showed that only ependymal cells produce glycosaminoglycan and proteoglycan-containing ECM, while the cathepsin studies showed both cell types remove ECM. Interaction of foam cells and ependymoglia in vitro supported the dispersion of ependymal outgrowth associated with tissue reconstruction in Axolotl spinal cord regeneration.
Asunto(s)
Ambystoma mexicanum/inmunología , Epéndimo/citología , Epéndimo/inmunología , Células Espumosas/inmunología , Meninges/citología , Meninges/inmunología , Regeneración de la Medula Espinal/inmunología , Ambystoma mexicanum/metabolismo , Animales , Catepsina K/inmunología , Femenino , Masculino , Vaina de Mielina/metabolismo , Médula Espinal/inmunologíaRESUMEN
It has been suggested that urate plays a protective role in neurons, while hyperuricemia is correlated with atherosclerosis and cardiovascular disease. However, whether there is a system that directly transports urate into the brain remains to be clarified. In this study, the localization of glucose transporter 9 (GLUT9) and urate transporter 1 (URAT1), which are known to be representative reabsorptive urate transporters, was immunohistochemically examined in autopsied human brains. Immunoreactivity of GLUT9 was observed on the apical side of the cytoplasm of epithelial cells in the choroid plexus and in the cilia of ependymal cells of the human brain. Immunoreactivity of URAT1 was observed on the basolateral side of the cytoplasm of epithelial cells in the choroid plexus. In addition, immunoreactivity of GLUT9 and URAT1 was not observed in microvessels of the human brains. The choroid plexus and renal proximal tubule were similar in having a polarized distribution of these two transporters with the two transporters on opposite membranes, but the two transporters' distribution differs between the choroid plexus and the kidney in terms of which membrane (apical/basal) expresses which transporter. These findings support the hypothesis of the direct transport of intravascular urate into the central nervous system through the choroid plexus.
Asunto(s)
Encéfalo/inmunología , Plexo Coroideo/inmunología , Células Epiteliales/inmunología , Proteínas Facilitadoras del Transporte de la Glucosa/análisis , Proteínas Facilitadoras del Transporte de la Glucosa/inmunología , Transportadores de Anión Orgánico/análisis , Transportadores de Anión Orgánico/inmunología , Proteínas de Transporte de Catión Orgánico/análisis , Proteínas de Transporte de Catión Orgánico/inmunología , Encéfalo/citología , Encéfalo/metabolismo , Plexo Coroideo/citología , Plexo Coroideo/metabolismo , Epéndimo/inmunología , Células Epiteliales/metabolismo , Humanos , Inmunohistoquímica , Túbulos Renales Proximales/inmunologíaRESUMEN
HSV type 1 (HSV-1) is one of the leading etiologies of sporadic viral encephalitis. Early antiviral intervention is crucial to the survival of herpes simplex encephalitis patients; however, many survivors suffer from long-term neurologic deficits. It is currently understood that HSV-1 establishes a latent infection within sensory peripheral neurons throughout the life of the host. However, the tissue residence of latent virus, other than in sensory neurons, and the potential pathogenic consequences of latency remain enigmatic. In the current study, we characterized the lytic and latent infection of HSV-1 in the CNS in comparison with the peripheral nervous system following ocular infection in mice. We used RT-PCR to detect latency-associated transcripts and HSV-1 lytic cycle genes within the brain stem, the ependyma (EP), containing the limbic and cortical areas, which also harbor neural progenitor cells, in comparison with the trigeminal ganglia. Unexpectedly, HSV-1 lytic genes, usually identified during acute infection, are uniquely expressed in the EP 60 d postinfection when animals are no longer suffering from encephalitis. An inflammatory response was also mounted in the EP by the maintenance of resident memory T cells. However, EP T cells were incapable of controlling HSV-1 infection ex vivo and secreted less IFN-γ, which correlated with expression of a variety of exhaustion-related inhibitory markers. Collectively, our data suggest that the persistent viral lytic gene expression during latency is the cause of the chronic inflammatory response leading to the exhaustion of the resident T cells in the EP.
Asunto(s)
Encefalitis por Herpes Simple/virología , Epéndimo/virología , Herpes Simple/inmunología , Linfocitos T/inmunología , Latencia del Virus/fisiología , Animales , Modelos Animales de Enfermedad , Encefalitis por Herpes Simple/inmunología , Epéndimo/inmunología , Citometría de Flujo , Herpesvirus Humano 1/fisiología , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Reacción en Cadena en Tiempo Real de la PolimerasaRESUMEN
The broad variety of substances that inhibit the action of the ubiquitin-proteasome system (UPS)-known as proteasome inhibitors-have been used extensively in previous studies, and they are currently frequently proposed as a novel form of cancer treatment and as a protective factor in intracerebral hemorrhage treatment. The experimental data on the safest route of proteasome inhibitor administration, their associated side effects, and the possible ways of minimizing these effects have recently become a very important topic. The aim of our present study was to determine the effects of administering of MG-132, lactacystin and epoxomicin, compounds belonging to three different classes of proteasome inhibitors, on the ependymal walls of the lateral ventricle. Observations were made 2 and 8 weeks after the intraventricular administration of the studied substances dissolved in dimethyl sulfoxide (DMSO) into the lateral ventricle of adult Wistar rats. Qualitative and quantitative analysis of brain sections stained with histochemical and inmmunofluorescence techniques showed that the administration of proteasome inhibitors caused a partial occlusion of the injected ventricle in all of the studied animals. The occlusion was due to ependymal cells damage and subsequent ependymal discontinuity, which caused direct contact between the striatum and the lateral nuclei of the septum, mononuclear cell infiltration and the formation of a glial scar between these structures (with the activation of astroglia, microglia and oligodendroglia). Morphologically, the ubiquitin-positive aggregates corresponded to aggresomes, indicating impaired activity of the UPS and the accumulation and aggregation of ubiquitinated proteins that coincided with the occurrence of glial scars. The most significant changes were observed in the wall covering the striatum in animals that were administered epoxomicin, and milder changes were observed in animals administered lactacystin and MG-132. Interestingly, DMSO administration also caused damage to some of the ependymal cells, but the aggresome-like structures were not formed. Our results indicate that all of the studied classes of proteasome inhibitors are detrimental to ependymal cells to some extent, and may cause severe changes in the ventricular system. The safety implications of their usage in therapeutic strategies to attenuate intracerebral hemorrhagic injury and in brain cancer treatment will require further studies.
Asunto(s)
Ventrículos Laterales/efectos de los fármacos , Inhibidores de Proteasoma/administración & dosificación , Inhibidores de Proteasoma/efectos adversos , Animales , Atrofia/inducido químicamente , Epéndimo/efectos de los fármacos , Epéndimo/inmunología , Epéndimo/metabolismo , Epéndimo/patología , Glioma Subependimario/inducido químicamente , Ventrículos Laterales/inmunología , Ventrículos Laterales/metabolismo , Ventrículos Laterales/patología , Masculino , Complejo de la Endopetidasa Proteasomal/metabolismo , Ratas , Ratas Wistar , Formación de Roseta , Ubiquitina/metabolismoRESUMEN
Glial cells, which constitute more than 50% of the mass of the central nervous system and greatly outnumber neurons, are at the vanguard of neuroendocrine research in metabolic control and obesity. Historically relegated to roles of structural support and protection, diverse functions have been gradually attributed to this heterogeneous class of cells with their protagonism in crescendo in all areas of neuroscience during the past decade. However, this dramatic increase in attention bestowed upon glial cells has also emphasized our vast lack of knowledge concerning many aspects of their physiological functions, let alone their participation in numerous pathologies. This minireview focuses on the recent advances in our understanding of how glial cells participate in the physiological regulation of appetite and systemic metabolism as well as their role in the pathophysiological response to poor nutrition and secondary complications associated with obesity. Moreover, we highlight some of the existing lagoons of knowledge in this increasingly important area of investigation.
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Astrocitos/metabolismo , Metabolismo Energético , Epéndimo/metabolismo , Homeostasis , Microglía/metabolismo , Obesidad/metabolismo , Animales , Astrocitos/inmunología , Epéndimo/citología , Epéndimo/inmunología , Epéndimo/patología , Humanos , Hipotálamo/citología , Hipotálamo/inmunología , Hipotálamo/metabolismo , Hipotálamo/patología , Microglía/inmunología , Obesidad/inmunología , Obesidad/patologíaRESUMEN
Epitope H contains an O-linked N-acetylglucosamine (O-GlcNAc) residue in a specific conformation and/or environment recognized by mouse IgM monoclonal antibody H (mabH). Epitope H is present in several types of cells and in several polypeptides outside the CNS. Previous results have shown that in the adult human brains, epitope H is confined mostly to a minority of fibrous astrocytes, and it is greatly upregulated in the reactive astrocytes. Post-translational modification with O-GlcNAc occurs on many proteins involved in several cell processes, such as cell cycle progression, apoptosis, proteasome degradation pathways, and modulation of cellular function in response to nutrition and stress. Hypoxia is one of the major causes of cellular stress. Therefore, in this study, we used the mAbH and the indirect immunoperoxidase method to investigate the expression of epitope H in ependymal cells in brains of persons who died with signs of hypoxic encephalopathy. The results of the present study showed that practically all ependymal cells showed cytoplasmic staining for epitope H in supranuclear cytoplasm in the brain of two premature neonates and in ten infants who died with signs of hypoxic encephalopathy. However, the overwhelming majority of ependymal cells of the nine human embryos taken from legal abortions, ranging from 26 days until 13 weeks of gestational age, and of the ten infants' brains without any sign of hypoxic encephalopathy remained negative. Only occasionally did the ependymal cells show weak cytoplasmic staining in some foci. In addition, the reactive astrocytes in the hypoxic brains showed strong cytoplasmic staining, confirming previous results.
Asunto(s)
Acetilglucosamina/análisis , Epéndimo/inmunología , Epítopos/análisis , Hipoxia Fetal/inmunología , Hipoxia Encefálica/inmunología , Anticuerpos Monoclonales , Astrocitos/inmunología , Citoplasma/inmunología , Epéndimo/embriología , Epéndimo/patología , Hipoxia Fetal/patología , Técnica del Anticuerpo Fluorescente Indirecta , Edad Gestacional , Humanos , Hipoxia Encefálica/embriología , Hipoxia Encefálica/mortalidad , Hipoxia Encefálica/patología , Recién Nacido , Recien Nacido Prematuro , Regulación hacia ArribaRESUMEN
Viral infection of the central nervous system can lead to disability and death. Yet the majority of viral infections with central nervous system involvement resolve with only mild clinical manifestations, if any. This is generally attributed to efficient elimination of the infection from the brain coverings, i.e. the meninges, ependyma and chorioplexus, which are the primary targets of haematogeneous viral spread. How the immune system is able to purge these structures from viral infection with only minimal detrimental effects is still poorly understood. In the present work we studied how an attenuated lymphocytic choriomeningitis virus can be cleared from the central nervous system in the absence of overt disease. We show that elimination of the virus from brain ependyma, but not from brain parenchyma, could be achieved by a T cell-dependent mechanism operating independently of major histocompatibility class I antigens and perforin. Considering that cytotoxic T lymphocyte-mediated cytotoxicity is a leading cause of viral immunopathology and tissue damage, our findings may explain why the most common viral intruders of the central nervous system rarely represent a serious threat to our health.
Asunto(s)
Encéfalo/inmunología , Epéndimo/inmunología , Antígenos de Histocompatibilidad Clase I/fisiología , Coriomeningitis Linfocítica/inmunología , Coriomeningitis Linfocítica/terapia , Perforina/fisiología , Linfocitos T/inmunología , Animales , Encéfalo/virología , Epéndimo/patología , Epéndimo/virología , Coriomeningitis Linfocítica/patología , Coriomeningitis Linfocítica/virología , Virus de la Coriomeningitis Linfocítica/inmunología , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Linfocitos T/virología , Linfocitos T Citotóxicos/inmunología , Linfocitos T Citotóxicos/virología , Virus de la Estomatitis Vesicular Indiana/inmunología , Carga Viral/inmunologíaRESUMEN
BACKGROUND AND PURPOSE: Recombinant interferon-beta is proven as an effective long-term treatment in patients with multiple sclerosis (MS). Unlike in other chronic inflammatory diseases, endogenous synthesis of type I interferons (IFN-alpha and IFN-beta) has not been studied extensively in MS. Mx proteins A and B (MxA and MxB) are intracellular proteins that are induced exclusively by type I IFNs. We investigated the expression of Mx proteins in post-mortem brain tissue of IFN-beta-naïve MS patients as a marker for endogenous synthesis of type I IFNs. METHODS: By employing monoclonal antibodies specific for MxA and MxB positive staining was detectable predominantly in reactive astrocytes within the MS plaques but also in endothelial and ependymal cells as well as in lymphocytic infiltrates. RESULTS: This is of interest in view of results previously published by our group and others that Mx protein concentrations measured by ELISA increase in blood samples from MS patients after IFN-beta therapy. CONCLUSIONS: In MS, Mx proteins are detectable in plaques suggesting endogenous synthesis of type I IFNs as part of the acute inflammatory process.
Asunto(s)
Encéfalo/metabolismo , Encefalitis/metabolismo , Proteínas de Unión al GTP/metabolismo , Interferón Tipo I/biosíntesis , Esclerosis Múltiple/metabolismo , Adulto , Anciano , Anticuerpos Monoclonales , Astrocitos/inmunología , Astrocitos/metabolismo , Astrocitos/patología , Biomarcadores/análisis , Biomarcadores/metabolismo , Encéfalo/inmunología , Encéfalo/patología , Quimiotaxis de Leucocito/inmunología , Encefalitis/inmunología , Encefalitis/fisiopatología , Células Endoteliales/inmunología , Células Endoteliales/metabolismo , Células Endoteliales/patología , Epéndimo/inmunología , Epéndimo/metabolismo , Epéndimo/patología , Femenino , Proteínas de Unión al GTP/análisis , Gliosis/inmunología , Gliosis/metabolismo , Gliosis/patología , Humanos , Inmunohistoquímica , Linfocitos/inmunología , Linfocitos/metabolismo , Linfocitos/patología , Masculino , Persona de Mediana Edad , Esclerosis Múltiple/inmunología , Esclerosis Múltiple/fisiopatología , Proteínas de Resistencia a Mixovirus , Adulto JovenRESUMEN
Neural stem cells (NSCs) in the subventricular zone (SVZ) continuously generate olfactory bulb interneurons in the adult rodent brain. Based on their ultrastructural and antigenic properties, NSCs, transient amplifying precursor cells, and neuroblasts (B, C, and A cells, respectively) have been distinguished in mouse SVZ. Here, we aimed to identify these cell types in rat SVZ ultrastructurally and at the light microscopy level, and to determine the antigenic properties of each cell type using gold and fluorescence immunolabeling. We found astrocytes with single cilia (NSCs, correspond to B cells) and neuroblasts (A cells). We also observed mitotic cells, ependymal cells, displaced ependymal cells, and mature astrocytes. In contrast, transient amplifying precursor cells (C cells) were not detected. The NSCs and neuroblasts had epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor alpha (PDGFRalpha) expressed on the ciliary apparatus and were the only cell types incorporating the proliferation marker BrdU. Throughout mitosis, EGFR and PDGFRalpha were associated with the microtubule of the mitotic spindle. Ependymal and displaced ependymal cells also expressed EGFR and PDGFRalpha on their cilia but did not incorporate BrdU. Our findings indicate that the NSCs in adult rat SVZ give rise directly to neuroblasts. During mitosis, the NSCs disassemble the primary cilium and symmetrically distribute EGFR and PDGFRalpha among their progeny.
Asunto(s)
Encéfalo/citología , Neurogénesis/fisiología , Neuronas/inmunología , Neuronas/ultraestructura , Células Madre/inmunología , Células Madre/ultraestructura , Animales , Astrocitos/inmunología , Astrocitos/metabolismo , Astrocitos/ultraestructura , Encéfalo/fisiología , Bromodesoxiuridina , Diferenciación Celular/fisiología , Proliferación Celular , Ventrículos Cerebrales/citología , Ventrículos Cerebrales/fisiología , Cilios/metabolismo , Cilios/ultraestructura , Epéndimo/inmunología , Epéndimo/metabolismo , Epéndimo/ultraestructura , Receptores ErbB/metabolismo , Inmunohistoquímica , Masculino , Microscopía Fluorescente , Microscopía Inmunoelectrónica , Mitosis/fisiología , Neuronas/metabolismo , Ratas , Ratas Wistar , Receptor alfa de Factor de Crecimiento Derivado de Plaquetas/metabolismo , Huso Acromático/metabolismo , Huso Acromático/ultraestructura , Células Madre/metabolismoRESUMEN
The subventricular zone of the striatal wall of adult rodents is an active neurogenic region for life. Cubic multiciliated ependyma separates the subventricular zone from the cerebrospinal fluid (CSF) and is involved in the control of adult neurogenesis. By injecting neuraminidase from Clostridium perfringens into the right lateral ventricle of the rat, we provoked a partial detachment of the ependyma in the striatal wall. The contralateral ventricle was never affected and was used as the experimental control. Neuraminidase caused widening of the intercellular spaces among some ependymal cells and their subsequent detachment and disintegration in the CSF. Partial ependymal denudation was followed by infiltration of the CSF with macrophages and neutrophils from the local choroid plexus, which ependymal cells never detached after neuraminidase administration. Inflammation extended toward the periventricular parenchyma. The ependymal cells that did not detach and remained in the ventricle wall never proliferated. The lost ependyma was never recovered, and ependymal cells never behaved as neural stem cells. Instead, a scar formed by overlapping astrocytic processes sealed those regions devoid of ependyma. Some ependymal cells at the border of the denudated areas lost contact with the ventricle and became located under the glial layer. Concomitantly with scar formation, some subependymal cells protruded toward the ventricle through the ependymal breaks, proliferated, and formed clusters of rounded ventricular cells that expressed the phenotype of neuroblasts. Ventricular clusters of neuroblasts remained in the ventricle up to 90 days after injection. In the subventricular zone, adult neurogenesis persisted.
Asunto(s)
Cuerpo Estriado/citología , Epéndimo/citología , Neuraminidasa/administración & dosificación , Neuronas/metabolismo , Células Madre/metabolismo , Animales , Diferenciación Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Cuerpo Estriado/efectos de los fármacos , Epéndimo/efectos de los fármacos , Epéndimo/inmunología , Inmunohistoquímica , Inflamación/inmunología , Inyecciones Intraventriculares , Masculino , Microscopía Confocal , Microscopía Electrónica , Neuronas/efectos de los fármacos , Neuronas/ultraestructura , Ratas , Ratas Wistar , Células Madre/efectos de los fármacos , Células Madre/ultraestructuraRESUMEN
Experimental autoimmune encephalomyelitis (EAE) animal models is an autoimmune demyelinating disease of the central nervous system, which is widely accepted as an animal for human multiple sclerosis (MS). Ependymal cells line the spinal canal and cerebral ventricles and proliferate in response to damage. These cells have the potential to differentiate into neural support cells. However, there is controversy as whether the response of the ependymal cells is a result of injury or repair. This study demonstrates using the rat EAE model a proliferative response of the ependymal cells occurred as a result of the disease. Interestingly, a more pronounced ependymal proliferative effect was seen in animals being fed a phase 2 enzyme inducer. The data suggests ependymal cells play a role in the post-inflammatory response of the brain and also may be involved in the remyelination process.
Asunto(s)
Encefalomielitis Autoinmune Experimental/inmunología , Encefalomielitis Autoinmune Experimental/patología , Epéndimo/inmunología , Epéndimo/patología , Inmunidad Innata/inmunología , Esclerosis Múltiple/inmunología , Esclerosis Múltiple/patología , Animales , Proliferación Celular , Femenino , Ratas , Ratas Endogámicas LewAsunto(s)
Epéndimo/citología , Inmunohistoquímica/métodos , Imitación Molecular , Proteínas no Estructurales Virales/inmunología , Animales , Anticuerpos Monoclonales/inmunología , Citoplasma/metabolismo , Epéndimo/inmunología , Epéndimo/metabolismo , Ratones , Ratones Endogámicos BALB C , Proteínas no Estructurales Virales/metabolismoRESUMEN
The habenular-interpeduncular pathway is involved in the modulation of several functions including neuroendocrine and stress responses. Interleukin-18 (IL-18) is a pro-inflammatory cytokine predominantly studied as a modulator of immune functions and also produced in the adrenal cortex following activation of the hypothalamic-pituitary-adrenal axis. In the central nervous system, IL-18 was demonstrated to induce sleep and to influence long-term potentiation and was proposed to mediate local inflammatory reactions. The present study investigated the localization of IL-18 and its expression following either acute or chronic restraint stress in the brain of adult male Wistar rats. Using immunocytochemistry and in situ hybridization we report the unprecedented localization of IL-18 in the neurons of the superior part of the medial habenula (MHbS), their projections to the interpenducular nucleus and its expression in the ependymal cells surrounding the third and the lateral ventricles. In addition, acute (2 h) or chronic (6 h/day for 3 weeks) restraint stress induced a strong elevation of IL-18 immunostaining in the MHbS but not in ependymal cells. The present data suggest that IL-18 may participate in the modulation of stress responses in the MHbS. They also suggest that ependymal cells may be the source of IL-18 previously reported in the cerebrospinal fluid (CSF). The role of IL-18 in the ependyma and the CSF remains to be elucidated.
Asunto(s)
Vías Eferentes/metabolismo , Epéndimo/metabolismo , Habénula/metabolismo , Interleucina-18/metabolismo , Neuroinmunomodulación/fisiología , Neuronas/metabolismo , Estrés Fisiológico/inmunología , Animales , Líquido Cefalorraquídeo/inmunología , Líquido Cefalorraquídeo/metabolismo , Vías Eferentes/citología , Vías Eferentes/inmunología , Epéndimo/citología , Epéndimo/inmunología , Habénula/citología , Habénula/inmunología , Inmunohistoquímica , Interleucina-18/genética , Interleucina-18/inmunología , Ventrículos Laterales/citología , Ventrículos Laterales/inmunología , Ventrículos Laterales/metabolismo , Masculino , Neuronas/citología , Neuronas/inmunología , ARN Mensajero/metabolismo , Ratas , Ratas Wistar , Estrés Fisiológico/líquido cefalorraquídeo , Estrés Fisiológico/metabolismo , Tercer Ventrículo/citología , Tercer Ventrículo/inmunología , Tercer Ventrículo/metabolismoRESUMEN
To test the hypothesis that macrophage migration inhibitory factor (MIF) plays a role in macrophage invasion during virus-induced encephalitis, we analyzed the expression and cellular localization of MIF in the Borna disease virus (BDV)-infected rat brain, monitored monocyte/macrophage infiltration, and evaluated the influence of anti-inflammatory treatment with dexamethasone. MIF mRNA expression was restricted to neurons and remained unchanged after BDV infection or after dexamethasone treatment of either BDV-infected or uninfected control rats. In contrast, MIF protein immunoreactivity (ir) was not only seen in neurons but also in glia. After BDV-induced encephalitis and treatment of uninfected rats with dexamethasone, MIF ir was only slightly altered in neurons but moderately enhanced in tanycytes, ependyma, and choroid plexus epithelium and markedly increased or induced in astrocyte end-feet at the blood-brain barrier (BBB). The increase in MIF ir in astrocytes after BDV infection was blocked by dexamethasone. The induction or enhancement of MIF ir at the BBB significantly correlated with reduced numbers of infiltrating ED1-positive monocytes/macrophages after BDV infection. Increased macrophage invasion was observed in regions where no astrocytic MIF was detected. The BDV- or dexamethasone-induced accumulation of MIF protein in astrocytes in vivo in absence of detectable astrocytic MIF mRNA expression is most likely due to MIF translocation from neurons rather than to a constitutive or induced MIF mRNA expression in astrocytes. In conclusion, we provide evidence that translocation of MIF from neurons or other extracellular sources into astrocytes is likely to modulate the inflammatory process during the course of virus-induced encephalitis by limiting monocyte/macrophage migration through the BBB.
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Astrocitos/metabolismo , Enfermedad de Borna/inmunología , Virus de la Enfermedad de Borna/inmunología , Encéfalo/inmunología , Encéfalo/virología , Movimiento Celular/inmunología , Factores Inhibidores de la Migración de Macrófagos/metabolismo , Macrófagos/inmunología , Animales , Antiinflamatorios no Esteroideos/farmacología , Astrocitos/inmunología , Astrocitos/virología , Barrera Hematoencefálica/efectos de los fármacos , Barrera Hematoencefálica/inmunología , Enfermedad de Borna/metabolismo , Enfermedad de Borna/fisiopatología , Virus de la Enfermedad de Borna/patogenicidad , Encéfalo/metabolismo , Movimiento Celular/efectos de los fármacos , Dexametasona/farmacología , Modelos Animales de Enfermedad , Ectodisplasinas , Encefalitis Viral/inmunología , Encefalitis Viral/metabolismo , Encefalitis Viral/fisiopatología , Endocitosis/efectos de los fármacos , Endocitosis/inmunología , Epéndimo/efectos de los fármacos , Epéndimo/inmunología , Epéndimo/metabolismo , Femenino , Factores Inhibidores de la Migración de Macrófagos/genética , Factores Inhibidores de la Migración de Macrófagos/farmacología , Macrófagos/metabolismo , Macrófagos/virología , Proteínas de la Membrana/inmunología , Proteínas de la Membrana/metabolismo , ARN Mensajero/efectos de los fármacos , ARN Mensajero/metabolismo , Ratas , Ratas Endogámicas Lew , Regulación hacia Arriba/efectos de los fármacos , Regulación hacia Arriba/inmunologíaRESUMEN
The biological effects of interleukin-1 (IL-1) are mediated by two distinct receptors, the p80 type I IL-1 and p68 type II IL-1 receptor proteins (IL-1RI and IL-1RII, respectively), both of which have been recently co-localized to the growth hormone synthesizing cells of the adenohypophysis. Previous studies have shown that IL-1 can bind to specific structures in the central nervous system, but the distribution of IL-1RI and IL-1RII proteins in the adult mouse brain has not been reported. Here we have used immunohistochemistry to study the expression, distribution and cellular localization of both isoforms of the IL-1 receptor proteins in the adult mouse brain. Using a combination of processing techniques (AMeX fixation and cryosectioning), we have immunolabeled brain sections for each isoform of the IL-1R. Both isoforms are expressed in the CNS, particularly in neuronal soma of the granular layer of the dentate gyrus and pyramidal cells of fields CA1-CA4 of Ammon's horn of the hippocampus, in epithelial cells of the choroid plexus and ependymal layer, and in neuronal soma of Purkinje cells of the cerebellum. The IL-1RII isoform, but not IL-1RI, is expressed in specific neuronal soma and proximal cell processes of neurons of the paraventricular gray matter of the hypothalamus. These immunohistochemical data directly demonstrate the neuronal expression of both IL-1R proteins in situ. The distribution and cellular localization of IL-1R proteins in the CNS provide a molecular basis for understanding reciprocal interactions between the immune system and the brain.
Asunto(s)
Química Encefálica/inmunología , Receptores de Interleucina-1/análisis , Factores de Edad , Animales , Plexo Coroideo/química , Plexo Coroideo/inmunología , Epéndimo/química , Epéndimo/inmunología , Femenino , Hipocampo/química , Hipocampo/inmunología , Inmunohistoquímica , Masculino , Ratones , Ratones Endogámicos , Peso Molecular , Neuritis/inmunología , Neuritis/metabolismo , Receptores de Interleucina-1/biosíntesis , Receptores de Interleucina-1/química , Receptores Tipo I de Interleucina-1 , Receptores Tipo II de Interleucina-1RESUMEN
The adult human ependyma expresses no intermediate filament proteins or secretory proteins; the fetal ependyma shows strong immunocytochemical (ICC) expression of vimentin, glial fibrillary acidic protein (GFAP), cytokeratins (CKs) of high molecular weight, glycoproteins, and S-100beta protein. Each has a precise and specific spatial distribution within the developing ependyma and a predictable time of appearance and regression in each region of the ventricular system. Several are coexpressed, but some appear earlier or persist longer than others. Secretory proteins of ependymal cells are important in several developmental processes such as the guidance of axonal growth cones. GFAP is not expressed in the floor plate ependyma at any stage of development, unlike vimentin and CK. The choroid plexus epithelium is a specialized ependyma, with an ICC profile that differs from the surface ependyma: vimentin, CK, and S-100beta protein continue to be expressed throughout fetal and adult life, but GFAP is not expressed. Certain cerebral malformations are associated with specific ICC abnormalities: ependymal S-100beta protein continues to be immunoreactive in disorders of neuroblast migration; ependymal vimentin is focally upregulated in Chiari malformations and congenital aqueductal stenosis. Other mammalian and nonmammalian species have characteristic profiles of ependymal immunoreactivity to the same proteins expressed in humans but exhibit interspecific differences.
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Plexo Coroideo/embriología , Epéndimo/embriología , Animales , Antígenos/inmunología , Diferenciación Celular , Plexo Coroideo/química , Plexo Coroideo/citología , Proteínas del Citoesqueleto/análisis , Proteínas del Citoesqueleto/genética , Desarrollo Embrionario y Fetal , Epéndimo/química , Epéndimo/citología , Epéndimo/inmunología , Histocitoquímica , Humanos , Inmunohistoquímica , Factores de Crecimiento Nervioso , Proteoglicanos/análisis , Subunidad beta de la Proteína de Unión al Calcio S100 , Proteínas S100/análisisRESUMEN
OBJECTIVE: Antiphospholipid antibodies (aPL), especially against phosphatidylserine and cardiolipin, are associated with a variety of neurological disorders. While it is believed aPL react with endothelial cells to cause cerebral thrombosis, it is not known to what degree aPL react with neural tissue nor which particular aPL specificities may be more relevant. We investigated direct aPL reactivity with the central nervous system (CNS) using 3 monoclonal IgM aPL that differentiate between cardiolipin and phosphatidylserine dependent antigens. METHODS: Brain and spinal cord from normal cat Felis domesticus and brain from CD-1 mice were reacted with aPL using indirect immunoperoxidase techniques. Monoclonal aPL were reacted with whole brain myelin by dot immunoblots. RESULTS: Monoclonal D11A4, reactive with cardiolipin and not phosphatidylserine (CL+/PS-), did not react with any portion of the tissue. Both monoclonal 3SB9b (CL-/PS+) and BA3B5C4 (CL+/PS+) reacted in feline and murine CNS. Both labeled myelinated fibers in grey and white matter of brain and spinal cord in an identical pattern with positive control antibody against myelin basic protein and reacted with whole human brain myelin by dot immunoblot. 3SB9b (CL-+PS+) additionally reacted with ependyma and epithelium of the choroid plexus. CONCLUSION: aPL, especially those reactive with phosphatidylserine dependent antigens, react directly with epitopes associated with myelin, brain ependyma, or choroid epithelium. Direct reactivity of aPL with nervous tissue may be relevant to neurological disorders.
Asunto(s)
Anticuerpos Antifosfolípidos/inmunología , Reacciones Antígeno-Anticuerpo/inmunología , Encéfalo/inmunología , Fosfatidilserinas/inmunología , Médula Espinal/inmunología , Animales , Anticuerpos Monoclonales/inmunología , Cardiolipinas/inmunología , Gatos , Corteza Cerebral/inmunología , Plexo Coroideo/inmunología , Epéndimo/inmunología , Humanos , Técnicas para Inmunoenzimas , Ratones , Proteínas de la Mielina/inmunología , Sensibilidad y EspecificidadRESUMEN
Complement is an important effector arm of the human immune response. Binding of proteolytic fragments derived from activation of complement by specific receptors leads to responses as diverse as inflammation, opsonization, and B-cell activation. The importance of characterizing the expression and regulation of complement in the CNS is highlighted by growing evidence that complement plays a significant role in the pathogenesis of a variety of neurological diseases, such as multiple sclerosis and Alzheimer's disease. In vitro studies have demonstrated that astrocytes, the predominant glial cell type in the brain, are capable of expressing or producing a majority of the components of the complement system. Expression of many complement proteins synthesized by astrocytes is regulated by both pro- and anti-inflammatory cytokines, many of which are also produced by several cell types in the CNS. In addition to astrocytes, ependymal cells, endothelial cells, microglia, and neurons have recently been shown to synthesize various complement proteins or express complement receptors on their cell surfaces. Together, these studies demonstrate that several cell types throughout the brain have the potential to express complement and, in many cases, increase expression in response to mediators of the acute phase response. These studies suggest that complement may play a greater role in CNS immune responses than previously thought, and pave the way for better understanding of the dynamics of complement expression and regulation in vivo. Such understanding may lead to therapeutic manipulation of complement host defense functions in a variety of inflammatory and degenerative diseases in the CNS.