RESUMEN
Understanding the role of horizontal gene transfer (HGT) in adaptation is a key challenge in evolutionary biology. In microbes, an important mechanism of HGT is prophage acquisition (phage genomes integrated into bacterial chromosomes). Prophages can influence bacterial fitness via the transfer of beneficial genes (including antibiotic-resistance genes, ARGs), protection from superinfecting phages, or switching to a lytic lifecycle that releases free phages infectious to competitors. We expect these effects to depend on environmental conditions because of, for example, environment-dependent induction of the lytic lifecycle. However, it remains unclear how costs/benefits of prophages vary across environments. Here, studying prophages with/without ARGs in Escherichia coli, we disentangled the effects of prophages alone and adaptive genes they carry. In competition with prophage-free strains, benefits from prophages and ARGs peaked in different environments. Prophages were most beneficial when induction of the lytic lifecycle was common, whereas ARGs were more beneficial upon antibiotic exposure and with reduced prophage induction. Acquisition of prophage-encoded ARGs by competing strains was most common when prophage induction, and therefore free phages, were common. Thus, selection on prophages and adaptive genes they carry varies independently across environments, which is important for predicting the spread of mobile/integrating genetic elements and their role in evolution.
Asunto(s)
Farmacorresistencia Bacteriana/genética , Escherichia coli K12/genética , Transferencia de Gen Horizontal , Aptitud Genética , Profagos/genética , Antibacterianos , Escherichia coli K12/virologíaRESUMEN
Bp7 is a T-even phage with a broad host range specific to Escherichia coli, including E. coli K-12. The receptor binding protein (RBP) of bacteriophages plays an important role in the phage adsorption process and determines phage host range, but the molecular mechanism involved in host recognition of phage Bp7 remains unknown. In this study, the interaction between phage Bp7 and E. coli K-12 was investigated. Based on homology alignment, amino acid sequence analysis, and a competitive assay, gp38, located at the tip of the long tail fiber, was identified as the RBP of phage Bp7. Using a combination of in vivo and in vitro approaches, including affinity chromatography, gene knockout mutagenesis, a phage plaque assay, and phage adsorption kinetics analysis, we identified the LamB and OmpC proteins on the surface of E. coli K-12 as specific receptors involved in the first step of reversible phage adsorption. Genomic analysis of the phage-resistant mutant strain E. coli K-12-R and complementation tests indicated that HepI of the inner core of polysaccharide acts as the second receptor recognized by phage Bp7 and is essential for successful phage infection. This observation provides an explanation of the broad host range of phage Bp7 and provides insight into phage-host interactions.IMPORTANCE The RBPs of T4-like phages are gp37 and gp38. The interaction between phage T4 RBP gp37 and its receptors has been clarified by many reports. However, the interaction between gp38 and its receptors during phage adsorption is still not completely understood. Here, we identified phage Bp7, which uses gp38 as an RBP, and provided a good model to study the phage-host interaction mechanisms in an enterobacteriophage. Our study revealed that gp38 of phage Bp7 recognizes the outer membrane proteins (OMPs) LamB and OmpC of E. coli K-12 as specific receptors and binds with them reversibly. HepI of the inner-core oligosaccharide is the second receptor and binds with phage Bp7 irreversibly to begin the infection process. Determining the interaction between the phage and its receptors will help elucidate the mechanisms of phage with a broad host range and help increase understanding of the phage infection mechanism based on gp38.
Asunto(s)
Proteínas de la Membrana Bacteriana Externa/genética , Colifagos/genética , Escherichia coli K12/virología , Lipopolisacáridos/metabolismo , Porinas/genética , Receptores Virales/genética , Secuencia de Aminoácidos , Proteínas de la Membrana Bacteriana Externa/metabolismo , Evolución Biológica , Colifagos/clasificación , Colifagos/metabolismo , Escherichia coli K12/genética , Escherichia coli K12/metabolismo , Prueba de Complementación Genética , Especificidad del Huésped , Lipopolisacáridos/química , Interacciones Microbianas/genética , Filogenia , Porinas/metabolismo , Receptores Virales/metabolismo , Alineación de Secuencia , Homología de Secuencia de AminoácidoRESUMEN
Many Escherichia coli phages have been sequenced, but in most cases their sequences alone do not suffice to predict their host specificity. Analysis of phage resistant E. coli K-12 mutants have uncovered a certain set of outer membrane proteins and polysaccharides as receptors. In this review, a compilation of E. coli K12 phage receptors is provided and their functional characterization, often driven by studies on phage resistant mutants, is discussed in the historical context. While great progress has been made in this field thus far, several proteins in the outer membrane still await characterization as phage receptors.
Asunto(s)
Proteínas de la Membrana Bacteriana Externa/metabolismo , Receptores de Bacteriógrafos/metabolismo , Colifagos/fisiología , Escherichia coli K12/metabolismo , Escherichia coli K12/virología , Proteínas de la Membrana Bacteriana Externa/genética , Receptores de Bacteriógrafos/genética , Colifagos/genética , Escherichia coli K12/genéticaRESUMEN
Anti-restriction proteins ArdB/KlcA specifically inhibit restriction (endonuclease) activity of restriction-modification (RM) type I systems. Molecular mechanisms of ArdB/KlcA-based anti-restriction remain unknown. In this study, we quantitate effects of ArdB on protection of unmodified λ phage DNA from EcoKI restriction. After UV irradiations, which produce significant amounts of unmodified chromosomal DNA in Escherichia coli K12 cells, the protective activity of ArdB decreases. Unlike ArdB, DNA-mimicking protein Ocr retains its ability to protect the unmodified λ phage regardless of UV dose. We hypothesize that the observed decrease in ArdB protective activity in UV-treated cells is due to its binding to unmodified chromosomal DNA, which decreases effective concentrations of free ArdB molecules available for λ phage protection against type I restriction enzymes.
Asunto(s)
Bacteriófago lambda/fisiología , Enzimas de Restricción del ADN/metabolismo , Escherichia coli K12/metabolismo , Escherichia coli K12/virología , Proteínas de Escherichia coli/inmunología , Bacteriófago lambda/genética , Enzimas de Restricción del ADN/genética , ADN Bacteriano/genética , ADN Bacteriano/metabolismo , Escherichia coli K12/genética , Escherichia coli K12/efectos de la radiación , Proteínas de Escherichia coli/genética , Rayos UltravioletaRESUMEN
The Keio single gene knockout collection comprises approximately 4000 mutants of E. coli K12 strain BW25113, where each mutant contains a kanamycin resistance cassette in place of a single nonessential gene. This mutant library has proven to be incredibly useful in the fields of bacteriology, chemical genomics, biotechnology, and systems biology, which is evidenced by the greater than 3800 citations that the article describing its construction has garnered in the approximate first 11 years since its publication. Among the various applications of the collection, the most extensive use has been in the assessment of how loss of specific gene function influences phenotypes. In this chapter, we describe pitfalls with use of the collection and procedures that can be employed to ensure robust phenotype assessment of mutations in the library. These include procedures for thorough confirmation of gene deletions by PCR, phage transduction of mutated loci to new host strains, and strategies for genetic complementation.
Asunto(s)
Escherichia coli K12/genética , Escherichia coli K12/metabolismo , Técnicas de Inactivación de Genes , Genes Bacterianos , Estudios de Asociación Genética , Fenotipo , Antibacterianos/farmacología , Bacteriófagos/fisiología , Escherichia coli K12/efectos de los fármacos , Escherichia coli K12/virología , Ingeniería Genética , Mutación , Reacción en Cadena de la Polimerasa , Transducción GenéticaRESUMEN
Antibiotic resistant pathogenic bacteria (ARPB) residual in soil-plant system has caused serious threat against public health and environmental safety. Being capable of targeted lysing host bacteria, phage therapy has been proposed as promising method to control the ARPB contamination in environments. In this study, microcosm trials were performed to investigate the impact of various phage treatments on the dissipation of tetracycline resistant Escherichia coli K-12 and chloramphenicol resistant Pseudomonas aeruginosa PAO1 in soil-carrot system. After 70â¯days of incubation, all the four phage treatments significantly decreased the abundance of the pathogenic bacteria and the corresponding antibiotic resistance genes (tetW and cmlA) in the soil-carrot system (pâ¯<â¯0.05), following the order of the cocktail phage treatment (phages ΦYSZ1â¯+â¯ΦYSZ2)â¯>â¯the polyvalent phage (ΦYSZ3 phage with broad host range) treatmentâ¯>â¯host-specific phage (ΦYSZ2 and ΦYSZ1) treatmentsâ¯>â¯the control. In addition, the polyvalent phage treatment also exerted positive impact on the diversity and stability of the bacterial community in the system, suggesting that this is an environmentally friendly technique with broad applications in the biocontrol of ARPB/ARGs in soil-plant system.
Asunto(s)
Bacteriófagos/fisiología , Farmacorresistencia Microbiana , Escherichia coli K12/virología , Pseudomonas aeruginosa/virología , Microbiología del Suelo , Biodiversidad , Agentes de Control Biológico , Daucus carota/microbiología , Escherichia coli K12/efectos de los fármacos , Escherichia coli K12/patogenicidad , Consorcios Microbianos , Pseudomonas aeruginosa/efectos de los fármacos , Pseudomonas aeruginosa/patogenicidad , Resistencia a la TetraciclinaRESUMEN
The vertical migration of antibiotic resistance pathogenic bacteria (ARPB) and antibiotic resistance genes (ARGs) in the surface soil-vadose soil system has become a new threat to ecological safety and public health; there is an imperative need to develop an efficient technique for targeted control and inactivation of ARPB in these systems. In this work, undisturbed soil columns (0 â¼ -5 m) were constructed to investigate the impact of biochar amendment or/and polyvalent bacteriophage (ΦYSZ-KK) therapy on the vertical control and inactivation of tetracycline-resistant Escherichia coli K-12 and chloramphenicol-resistant Klebsiella pneumonia K-6. The simultaneous application of polyvalent phage and biochar impeded the vertical migration of ARPB from the top soil to lower soil layers and stimulated the ARPB dissipation in the soil column. After 60-day incubation, levels of ARPB and ARGs decreased significantly in the soil column by magnitudes of 2-6. Additionally, high throughput sequencing indicated that the simultaneous application of biochar and phage clearly maintained the structure and diversity of the soil microbial communities (p < 0.05). This work therefore demonstrates that the application of a biochar/phage combination is an environmentally friendly, efficacious measure for the control and inactivation of ARPB/ARGs in vertical soil column systems.
Asunto(s)
Bacteriófagos , Carbón Orgánico , Farmacorresistencia Bacteriana , Escherichia coli K12/virología , Klebsiella pneumoniae/virología , Antibacterianos , Cloranfenicol , Farmacorresistencia Bacteriana/genética , Genes Bacterianos , Riesgo , Microbiología del Suelo , Contaminantes del Suelo , TetraciclinaRESUMEN
Bistable switches are common gene regulatory motifs directing two mutually exclusive cell fates. Theoretical studies suggest that bistable switches are sufficient to encode more than two cell fates without rewiring the circuitry due to the non-equilibrium, heterogeneous cellular environment. However, such a scenario has not been experimentally observed. Here by developing a new, dual single-molecule gene-expression reporting system, we find that for the two mutually repressing transcription factors CI and Cro in the classic bistable bacteriophage λ switch, there exist two new production states, in which neither CI nor Cro is produced, or both CI and Cro are produced. We construct the corresponding potential landscape and map the transition kinetics among the four production states. These findings uncover cell fate potentials beyond the classical picture of bistable switches, and open a new window to explore the genetic and environmental origins of the cell fate decision-making process in gene regulatory networks.
Asunto(s)
Bacteriófago lambda/genética , Escherichia coli K12/genética , Redes Reguladoras de Genes , Genes de Cambio , Proteínas Represoras/genética , Proteínas Reguladoras y Accesorias Virales/genética , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Bacteriófago lambda/metabolismo , Escherichia coli K12/metabolismo , Escherichia coli K12/virología , Genes Reporteros , Proteínas Luminiscentes/genética , Proteínas Luminiscentes/metabolismo , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Proteínas Represoras/metabolismo , Proteínas Reguladoras y Accesorias Virales/metabolismo , Proteína Fluorescente RojaRESUMEN
Ability to efficiently propagate high quantities of bacteriophages (phages) is of great importance considering higher phage production needs in the future. Continuous production of phages could represent an interesting option. In our study, we tried to elucidate the effect of dilution rate on productivity of continuous production of phages in cellstat. As a model system, a well-studied phage T4 and Escherichia coli K-12 as a host were used. Experiments where physiology of bacteria was changing with dilution rate of cellstat and where bacterial physiology was kept constant were performed. For both setups there exists an optimal dilution rate when maximal productivity is achieved. Experimentally obtained values of phage concentration and corresponding productivity were compared with mathematical model predictions, and good agreement was obtained for both types of experiments. Analysis of mathematical model coefficients revealed that latent period and burst size to dilution rate coefficient mostly affect optimum dilution rate and productivity. Due to high sensitivity, it is important to evaluate phage growth parameters carefully, to run cellstat under optimal productivity.
Asunto(s)
Bacteriófagos/fisiología , Reactores Biológicos , Escherichia coli K12/virología , Cultivo de Virus/métodos , Modelos BiológicosRESUMEN
It is important to understand how physiological state of the host influence propagation of bacteriophages (phages), due to the potential higher phage production needs in the future. In our study, we tried to elucidate the effect of bacterial growth rate on adsorption constant (δ), latent period (L), burst size (b), and bacteriophage population growth rate (λ). As a model system, a well-studied phage T4 and Escherichia coli K-12 as a host was used. Bacteria were grown in a continuous culture operating at dilution rates in the range between 0.06 and 0.98 hr-1 . It was found that the burst size increases linearly from 8 PFU·cell-1 to 89 PFU·cell-1 with increase in bacteria growth rate. On the other hand, adsorption constant and latent period were both decreasing from 2.6â10-9 ml·min-1 and 80 min to reach limiting values of 0.5 × 10-9 ml·min-1 and 27 min at higher growth rates, respectively. Both trends were mathematically described with Michaelis-Menten based type of equation and reasons for such form are discussed. By applying selected equations, a mathematical equation for prediction of bacteriophage population growth rate as a function of dilution rate was derived, reaching values around 8 hr-1 at highest dilution rate. Interestingly, almost identical description can be obtained using much simpler Monod type equation and possible reasons for this finding are discussed.
Asunto(s)
Bacteriófago T4/crecimiento & desarrollo , Escherichia coli K12/crecimiento & desarrollo , Crecimiento Demográfico , Escherichia coli K12/virología , Ensayo de Placa Viral , Latencia del Virus/fisiologíaRESUMEN
Enteric viruses encounter diverse environments as they migrate through the gastrointestinal tract to infect their hosts. The interaction of eukaryotic viruses with members of the host microbiota can greatly impact various aspects of virus biology, including the efficiency with which viruses can infect their hosts. Mammalian orthoreovirus, a human enteric virus that infects most humans during childhood, is negatively affected by antibiotic treatment prior to infection. However, it is not known how components of the host microbiota affect reovirus infectivity. In this study, we show that reovirus virions directly interact with Gram positive and Gram negative bacteria. Reovirus interaction with bacterial cells conveys enhanced virion thermostability that translates into enhanced attachment and infection of cells following an environmental insult. Enhanced virion thermostability was also conveyed by bacterial envelope components lipopolysaccharide (LPS) and peptidoglycan (PG). Lipoteichoic acid and N-acetylglucosamine-containing polysaccharides enhanced virion stability in a serotype-dependent manner. LPS and PG also enhanced the thermostability of an intermediate reovirus particle (ISVP) that is associated with primary infection in the gut. Although LPS and PG alter reovirus thermostability, these bacterial envelope components did not affect reovirus utilization of its proteinaceous cellular receptor junctional adhesion molecule-A or cell entry kinetics. LPS and PG also did not affect the overall number of reovirus capsid proteins σ1 and σ3, suggesting their effect on virion thermostability is not mediated through altering the overall number of major capsid proteins on the virus. Incubation of reovirus with LPS and PG did not significantly affect the neutralizing efficiency of reovirus-specific antibodies. These data suggest that bacteria enhance reovirus infection of the intestinal tract by enhancing the thermal stability of the reovirus particle at a variety of temperatures through interactions between the viral particle and bacterial envelope components.
Asunto(s)
Bacillus subtilis/fisiología , Enterocitos/virología , Escherichia coli K12/fisiología , Infecciones por Reoviridae/virología , Reoviridae/fisiología , Acetilglucosamina/análogos & derivados , Acetilglucosamina/metabolismo , Acetilglucosamina/toxicidad , Bacillus subtilis/metabolismo , Bacillus subtilis/ultraestructura , Bacillus subtilis/virología , Células CACO-2 , Endotoxinas/metabolismo , Endotoxinas/toxicidad , Enterocitos/efectos de los fármacos , Enterocitos/microbiología , Enterocitos/patología , Escherichia coli K12/metabolismo , Escherichia coli K12/ultraestructura , Escherichia coli K12/virología , Microbioma Gastrointestinal , Células HeLa , Calor , Humanos , Lipopolisacáridos/metabolismo , Lipopolisacáridos/toxicidad , Proteínas Luminiscentes/genética , Proteínas Luminiscentes/metabolismo , Microscopía Electrónica de Transmisión , Peptidoglicano/metabolismo , Peptidoglicano/toxicidad , ARN/metabolismo , Estabilidad del ARN/efectos de los fármacos , Proteínas Recombinantes/metabolismo , Reoviridae/química , Reoviridae/efectos de los fármacos , Reoviridae/patogenicidad , Infecciones por Reoviridae/metabolismo , Infecciones por Reoviridae/microbiología , Infecciones por Reoviridae/patología , Ácidos Teicoicos/metabolismo , Ácidos Teicoicos/toxicidad , Virión/química , Virión/patogenicidad , Virión/fisiología , Acoplamiento Viral/efectos de los fármacos , Internalización del Virus/efectos de los fármacos , Proteína Fluorescente RojaRESUMEN
Subclonal analysis of spontaneous zygogenesis (Z-mating) products of Escherichia coli K12 was undertaken to grasp the extent of vertical and horizontal evolution in unstable strains expressing one parental or recombinant genome. Isolates were obtained following serial cultures or serial intercrosses. A high diversity of strains was obtained, among which some resumed the phenotype of the partners of the initial or subsequent Z-matings. When non-complementing diploids are infected with a mixture of distinct temperate bacteriophages, lysogenisation occurs at the expenses of the active chromosome only. This event is associated with an alternate expression of prophage and chromosomal genes. Present work provides further evidence for the existence of non-complementing diploidy and opens a novel route for virus research in general.
Asunto(s)
Colifagos/genética , Cruzamientos Genéticos , Escherichia coli K12/genética , Escherichia coli K12/virología , Profagos/genética , Recombinación Genética , Diploidia , Escherichia coli K12/fisiología , Evolución Molecular , Expresión Génica , Genotipo , Lisogenia , Fenotipo , Pase SeriadoRESUMEN
Many studies on phage biology are based on isolation methods that may inadvertently select for narrow-host-range phages. Consequently, broad-host-range phages, whose ecological significance is largely unexplored, are consistently overlooked. To enhance research on such polyvalent phages, we developed two sequential multihost isolation methods and tested both culture-dependent and culture-independent phage libraries for broad infectivity. Lytic phages isolated from activated sludge were capable of interspecies or even interorder infectivity without a significant reduction in the efficiency of plating (0.45 to 1.15). Two polyvalent phages (PX1 of the Podoviridae family and PEf1 of the Siphoviridae family) were characterized in terms of adsorption rate (3.54 × 10(-10) to 8.53 × 10(-10) ml/min), latent time (40 to 55 min), and burst size (45 to 99 PFU/cell), using different hosts. These phages were enriched with a nonpathogenic host (Pseudomonas putida F1 or Escherichia coli K-12) and subsequently used to infect model problematic bacteria. By using a multiplicity of infection of 10 in bacterial challenge tests, >60% lethality was observed for Pseudomonas aeruginosa relative to uninfected controls. The corresponding lethality for Pseudomonas syringae was â¼ 50%. Overall, this work suggests that polyvalent phages may be readily isolated from the environment by using different sequential hosts, and this approach should facilitate the study of their ecological significance as well as enable novel applications.
Asunto(s)
Bacteriófagos/aislamiento & purificación , Bacteriófagos/fisiología , Especificidad del Huésped , Aguas del Alcantarillado/virología , Cultivo de Virus/métodos , Bacteriófagos/clasificación , Bacteriófagos/patogenicidad , ADN Viral , Escherichia coli K12/virología , Podoviridae/aislamiento & purificación , Podoviridae/fisiología , Fagos Pseudomonas/aislamiento & purificación , Fagos Pseudomonas/fisiología , Pseudomonas aeruginosa/virología , Pseudomonas putida/virología , Pseudomonas syringae/virología , Siphoviridae/aislamiento & purificación , Siphoviridae/fisiologíaRESUMEN
We describe a genetic ß-galactoside reporter system using a disk diffusion assay on MacConkey Lactose agar petri plates to monitor maintenance of the bacteriophage λ prophage state and viral induction in Escherichia coli K-12. Evidence is presented that the phage λ major lytic promoters, pL and pR, are activated when cells containing the reporters are exposed to the energy poison carbonyl cyanide m-chlorophenyl hydrazine, CCCP. This uncoupler of oxidative phosphorylation inhibits ATP synthesis by collapsing the proton motive force. Expression of the λ lytic promoters in response to CCCP requires host RecA function and an autocleavable CI repressor, as does SOS induction of the λ prophage that occurs by a DNA damage-dependent pathway. λ Cro function is required for CCCP-mediated activation of the λ lytic promoters. CCCP does not induce an sfi-lacZ SOS reporter.
Asunto(s)
Bacteriófago lambda/efectos de los fármacos , Bacteriófago lambda/fisiología , Carbonil Cianuro m-Clorofenil Hidrazona/metabolismo , Escherichia coli K12/efectos de los fármacos , Escherichia coli K12/virología , Lisogenia/efectos de los fármacos , Activación Viral/efectos de los fármacos , Fusión Artificial Génica , Genes Reporteros , Regiones Promotoras Genéticas , Fuerza Protón-Motriz/efectos de los fármacos , beta-Galactosidasa/análisis , beta-Galactosidasa/genéticaRESUMEN
Rac or rac-like prophage harbors many genes with important physiological functions, while it remains excision-proficient in several bacterial strains including Escherichia coli, Salmonella spp. and Shigella spp. Here, we found that rac excision is induced during biofilm formation, and the isogenic stain without rac is more motile and forms more biofilms in nutrient-rich medium at early stages in E. coli K-12. Additionally, the presence of rac genes increases cell lysis during biofilm development. In most E. coli strains, rac is integrated into the ttcA gene which encodes a tRNA-thioltransferase. Rac excision in E. coli K-12 leads to a functional change of TtcA, which results in reduced fitness in the presence of carbenicillin. Additionally, we demonstrate that YdaQ (renamed as XisR) is the excisionase of rac in E. coli K-12, and that rac excision is induced by the stationary sigma factor RpoS through inducing xisR expression. Taken together, our results reveal that upon rac integration, not only are new genes introduced into the host, but also there is a functional change in a host enzyme. Hence, rac excision is tightly regulated by host factors to control its stability in the host genome under different stress conditions.
Asunto(s)
Biopelículas/crecimiento & desarrollo , ADN Nucleotidiltransferasas/metabolismo , Escherichia coli K12/crecimiento & desarrollo , Profagos/metabolismo , Proteínas Virales/metabolismo , Activación Viral/genética , Liberación del Virus/genética , Secuencia de Aminoácidos , Proteínas Bacterianas/genética , Carbenicilina/farmacología , ADN Nucleotidiltransferasas/genética , Escherichia coli K12/efectos de los fármacos , Escherichia coli K12/genética , Escherichia coli K12/virología , Proteínas de Escherichia coli/genética , Regulación Bacteriana de la Expresión Génica/genética , Regulación Viral de la Expresión Génica/genética , Datos de Secuencia Molecular , Profagos/genética , Alineación de Secuencia , Factor sigma/genética , Sulfurtransferasas/genética , Proteínas Virales/genética , Activación Viral/fisiologíaRESUMEN
The phage induced disinfection rates of Escherichia coli K-12 MG1655 in the presence of coliphage Ec2 were determined under a wide range of phage and bacterial concentrations. These rates were elucidated to determine if phages could be used in water and wastewater treatment systems as a biological based disinfectant. Disinfection rates ranging from 0.13 ± 0.1 to 2.03 ± 0.1 h⻹ were observed for E. coli K12. A multiple linear regression model was used to explain the variance in the disinfection rates, and this model demonstrated an interaction effect between the initial phage and bacterial concentrations. Furthermore, the results were modeled with particle aggregation theory, which over predicted the disinfection rates at higher phage and bacterial concentrations, suggesting additional interactions. Finally, the observed and predicted disinfection rates were used to determine additional design parameters. The results suggested that a phage based disinfection process may be suitable for the inactivation of specific pathogens in plug flow reactors, such as the pathogens in hospital wastewater effluents and the bacteria responsible for foaming and sludge bulking in activated sludge processes.
Asunto(s)
Colifagos/fisiología , Desinfección/métodos , Escherichia coli K12/virología , Interacciones Microbianas , Algoritmos , Reactores Biológicos/microbiología , Reactores Biológicos/virología , Recuento de Colonia Microbiana , Estudios de Factibilidad , Interacciones Huésped-Patógeno , Modelos Lineales , Eliminación de Residuos Sanitarios/métodos , Modelos Biológicos , Reproducibilidad de los Resultados , Aguas del Alcantarillado/microbiología , Aguas del Alcantarillado/virología , Factores de Tiempo , Ensayo de Placa ViralRESUMEN
The genome of transposable phage Mu is packaged as a linear segment, flanked by several hundred base pairs of non-Mu DNA. The linear ends are held together and protected from nucleases by the phage N protein. After transposition into the Escherichia coli chromosome, the flanking DNA (FD) is degraded, and the 5-bp gaps left in the target are repaired to generate a simple Mu insertion. Our study provides insights into this repair pathway. The data suggest that the first event in repair is removal of the FD by the RecBCD exonuclease, whose entry past the N-protein block is licensed by the transpososome. In vitro experiments reveal that, when RecBCD is allowed entry into the FD, it degrades this DNA until it arrives at the transpososome, which presents a barrier for further RecBCD movement. RecBCD action is required for stimulating endonucleolytic cleavage within the transpososome-protected DNA, leaving 4-nt flanks outside both Mu ends. This end product of collaboration between the transpososome and RecBCD resembles the intermediate products of Tn7 and retroviral and retrotransposon transposition, and may hint at a common gap-repair mechanism in these diverse transposons.
Asunto(s)
Bacteriófago mu/genética , Bacteriófago mu/metabolismo , Elementos Transponibles de ADN/genética , Exodesoxirribonucleasa V/metabolismo , Sustitución de Aminoácidos , Reparación del ADN , ADN Viral/genética , ADN Viral/metabolismo , Escherichia coli K12/genética , Escherichia coli K12/metabolismo , Escherichia coli K12/virología , Células HEK293 , Humanos , Modelos Biológicos , Mutagénesis Sitio-Dirigida , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Especificidad de la Especie , Transposasas/química , Transposasas/genética , Transposasas/metabolismo , Proteínas Virales/química , Proteínas Virales/genética , Proteínas Virales/metabolismoRESUMEN
Bacteriophages are considered as attractive indicators for determining drinking water quality since its concentration is strongly correlated with virus concentrations in water samples. Previously, bacteriophage detection was based on a plague assay that required a complicated labelling technique and a time-consuming culture assay. Here, for the first time, a label-free bacteriophage detection is reported by using droplet optofluidic imaging, which uses host-cell-containing microdroplets as reaction carriers for bacteriophage infection due to a higher contact ratio. The optofluidic imaging is based on the effective refractive index changes in the microdroplet correlated with the growth rate of the infected host cells, which is highly sensitive, i.e. can detect one E. coli cell. The droplet optofluidic system is not only used in drinking water quality monitoring, but also has high potential applications for pathogenic bacteria detection in clinical diagnosis and food industry.
Asunto(s)
Bacteriófago lambda , Microbiología de Alimentos , Luz , Técnicas Analíticas Microfluídicas , Dispersión de Radiación , Técnicas de Cocultivo , Escherichia coli K12/crecimiento & desarrollo , Escherichia coli K12/virología , Microbiología de Alimentos/instrumentación , Microbiología de Alimentos/métodos , Técnicas Analíticas Microfluídicas/métodosRESUMEN
AIM: Determine the possibility of Iysogenization of Escherichia coli single strain DNA (ssDNA) by 1ø7 bacteriophage from the Microviridae family and determine the role of phage lø7 lysogeny in genetic variability of these bacteria. MATERIALS AND METHODS: A method of E. coli K12 lysogenization by phage lø7 was developed. A spot-test for the control of resistance of the obtained lysogens against phage lø7 and determination of lysogen lø7 spontaneous production was worked out. Criteria for phage lø7 identification, that is spontaneously produced by E. coli K12 lysogens, were proposed. A kit of isogenic E. coli strains, that vary by mutations in ptsI, ptsH and fruA genes, that code phosphoenolpyruvate (PEP): carbohydrate phosphotransferase system (PTS) proteins, was constructed. RESULTS: The ability of highly virulent bacteriophage lø7 to lysogenize E. coli was shown. A reduction of lø7 titers in ptsI, ptsH and fruA E. coli K12 mutants was demonstrated compared with titers in wild-type bacteria. Lytic bacteriophage lø7 was also able to lysogenize ptsI, ptsH and fruA mutants at a high frequency. Lysogens are resistant to phages lø7, phiX174 of Microvirus genus and spontaneously produce lø7. CONCLUSION: Bacteriophage lø7 of the Microviridae family is able to lysogenize E. coli K12 and vertically transfer genome of this lytic phage. As a result, lytic phage lø7 takes part in bacterial variability as a factor of lysogen selection in bacteria population corresponding to PTS mutants by phenotype.
Asunto(s)
Cromosomas Bacterianos , Colifagos/genética , Escherichia coli K12/virología , Regulación Bacteriana de la Expresión Génica , Variación Genética , Lisogenia/genética , Proteínas Bacterianas/genética , Mapeo Cromosómico , Colifagos/patogenicidad , ADN Bacteriano/genética , ADN de Cadena Simple/genética , Escherichia coli K12/genética , Proteínas de Escherichia coli/genética , Transferencia de Gen Horizontal , Genotipo , Interacciones Huésped-Patógeno , Proteínas de Transporte de Monosacáridos/genética , Mutación , Fenotipo , Sistema de Fosfotransferasa de Azúcar del Fosfoenolpiruvato/genéticaRESUMEN
The Ocr protein, encoded by 0.3 (ocr) gene of bacteriophage T7, belongs to the family of antirestriction proteins that specifically inhibit the type I restriction-modification systems. Native Ocr forms homodimer (Ocr)2 both in solution and in the crystalline state. The Ocr protein belongs to the family of mimicry proteins. F53D A57E and E53R V77D mutant proteins were obtained, which form monomers. It was shown that the values of the dissociation constants Kd for Ocr, Ocr F53D A57E and Ocr F53RV77D proteins with EcoKI enzyme differ in 1000 times: Kd (Ocr) = 10(-10) M, Kd (Ocr F53D A57E and Ocr F53R V77D) = 10(-7) M. Antimodification activity of the Ocr monomeric forms is significantly reduced. We have shown, that Ocr dimeric form has fundamental importance for high inhibitory activity.