RESUMEN
Acute thromboembolic events appear to be frequent in patients with SARS-CoV-2 infection. We report a case of an intubated patient, who developed a threatening lower limb ischemia. Intra-arterial fibrinolysis and intravenous heparin infusion did not lead to complete recanalization of the tibial arteries, which were successfully treated by surgical embolectomy.
Asunto(s)
Infecciones por Coronavirus/patología , Isquemia/virología , Extremidad Inferior/fisiopatología , Neumonía Viral/patología , Aorta/patología , Betacoronavirus , COVID-19 , Fibrinolíticos/uso terapéutico , Heparina/uso terapéutico , Humanos , Isquemia/cirugía , Extremidad Inferior/virología , Masculino , Persona de Mediana Edad , Pandemias , SARS-CoV-2 , Trombosis , Tibia/irrigación sanguíneaRESUMEN
BACKGROUND: We report a case that presented as fever with positive Epstein-Barr Virus (EBV) IgM antibody combined with subcutaneous nodules on lower extremities and cervical lymphadenopathy firstly misdiagnosed as infectious mononucleosis, which was proven as subcutaneous panniculitis-like T-cell lymphoma by subcutaneous nodule biopsies. METHODS: Appropriate serum and bacteriological laboratory tests were carried out for the cause of fever. An ultrasound and subcutaneous nodule biopsies were performed. RESULTS: EBV IgM antibody was positive. An ultrasound revealed multiple subcutaneous nodules, which were prone to be lipoma on lower extremities and cervical lymphadenopathy. Subcutaneous nodule biopsies were firstly misdiagnosed as lipoma, while pathology consultation for the subcutaneous nodule biopsies diagnosed subcutaneous panniculitis-like T-cell lymphoma. CONCLUSIONS: When patients have persistent fever with positive EBV IgM antibody combined other system involvements, especially lymphadenopathy and multiple subcutaneous nodules, it should differentiate lymphoma from infectious diseases.
Asunto(s)
Fiebre/diagnóstico , Inmunoglobulina M/inmunología , Mononucleosis Infecciosa/diagnóstico , Extremidad Inferior/patología , Linfadenopatía/diagnóstico , Linfoma de Células T/diagnóstico , Paniculitis/diagnóstico , Tejido Subcutáneo/patología , Adulto , Anticuerpos Antivirales/inmunología , Biopsia , Diagnóstico Diferencial , Femenino , Fiebre/etiología , Herpesvirus Humano 4/inmunología , Herpesvirus Humano 4/fisiología , Humanos , Mononucleosis Infecciosa/complicaciones , Mononucleosis Infecciosa/virología , Extremidad Inferior/virología , Linfadenopatía/etiología , Linfoma de Células T/complicaciones , Cuello , Paniculitis/complicaciones , Derivación y Consulta , Tejido Subcutáneo/virologíaRESUMEN
BACKGROUND: The aim of this study was to assess how anterior cruciate ligament (ACL) ruptures with subsequent surgery reconstruction impact on the professional career of A1-A2 Italian women's volleyball league players. METHODS: Using an observational study with a retrospective case-series design for ACL ruptures, 125 teams with 1488 players were monitored. Subjects had to report level, role, injury modality, lower limb injured, laterality, period of the season and age. RESULTS: A total of 34 ACL ruptures were reported. Thirty-three (97%) were non-contact and 1 (3%) with contact. Twenty-one (61.7%) occurred in landing from a jump attack, 3 (8.8%) in landing from wall jump, 1 (3%) with apparent contact and 9 (26.5%) in other landing conditions. The most injured knee was the left limb (22, 64.7%) respect to the right limb (12, 35.3%). Fourteen (41.2%) ruptures occurred in spikers, 10 (29.4%) in middle blockers, 6 (17.6%) in setters, 3 (8.8%) in liberos and 1 (3%) in opposite hitters. Nine (26.5%) occurred in pre-season period, 16 (47%) in the first round, 4 (11.8%) in the second round, and 5 (14.7%) during play-off. The average age of the first ACL rupture was 23±3 years. CONCLUSIONS: We observed that female volleyball players of A1-A2 Italian volleyball league occurred mostly in a left non-contact ACL rupture during a landing condition and the spikers were the players most at risk. Therefore, it is desirable that coaches teach players variations of landing in order to avoid possible chronic overloading of ACL.
Asunto(s)
Lesiones del Ligamento Cruzado Anterior/epidemiología , Voleibol/lesiones , Adolescente , Adulto , Lesiones del Ligamento Cruzado Anterior/cirugía , Femenino , Humanos , Italia/epidemiología , Traumatismos de la Rodilla/epidemiología , Traumatismos de la Rodilla/cirugía , Extremidad Inferior/lesiones , Extremidad Inferior/virología , Estudios Retrospectivos , Voleibol/estadística & datos numéricos , Adulto JovenRESUMEN
In vivo infection of mosquitoes is an important method to study and characterize arthropod-borne viruses. Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that is transmitted primarily by Aedes mosquitoes. In this chapter, we describe a protocol for infection of CHIKV in two species of Aedes mosquitoes, Aedes aegypti and Aedes albopictus, together with the isolation of CHIKV in different parts of the infected mosquito such as midgut, legs, wings, salivary gland, head, and saliva. This allows the study of viral infection, replication and dissemination within the mosquito vector.
Asunto(s)
Aedes/virología , Infecciones por Alphavirus/virología , Virus Chikungunya/patogenicidad , Animales , Virus Chikungunya/aislamiento & purificación , Virus Chikungunya/fisiología , Femenino , Cabeza/virología , Humanos , Insectos Vectores/virología , Intestinos/virología , Extremidad Inferior/virología , Saliva/virología , Glándulas Salivales/virología , Replicación Viral , Alas de Animales/virologíaRESUMEN
With the aim of simplifying recombinant-adeno-associated virus (rAAV) delivery in muscle, a new femoral intra-arterial technique was designed and tested in rodents (rats and mice). Two serotypes, several promoters and transgenes (reporter or therapeutic) were tested using this administration route. The new route is both easy to perform and efficient. Its usefulness as a tool to assess gene delivery constructs in the muscle was established in the context of recombinant AAV serotypes 1 and 2, and with the ubiquitous CMV and two muscle-specific (C5-12 and CK6) promoters. Both serum monitoring of a secreted protein (murine alkaline phosphatase: muSEAP) and slide staining were used to compare the different constructs. Significantly different patterns of expression in kinetics of expression (muSEAP) and homogeneity of fiber transduction (staining) were evidenced with the different promoters tested, and compared with intra-muscular expression patterns. Detailed studies of differential transduction in leg and thigh muscles showed equivalent efficacy, except in rectus femoris, and to a lesser extent in soleus. In light of these results and prior data, intra-arterially mediated gene transfer mechanism is discussed.