RESUMEN
To test the hypothesis that ultraviolet radiation (UV) modulates photosynthetic responses to elevated CO2 concentration ([CO2]) in plants, saplings of European beech were grown for two vegetation seasons under ambient (400â¯ppm) and elevated (700â¯ppm) atmospheric [CO2]. From April to November the saplings were exposed to (i) ambient UV radiation, (ii) excluded and (iii) enhanced UV (150% of ambient). Gas-exchange and chlorophyll fluorescence techniques were used throughout the second vegetation season together with biochemical analyses of the amount and activity of the Rubisco enzyme. We found support for the hypothesis that an impact of elevated [CO2] on photosynthesis is substantially modulated by UV radiation. Moreover, we found that the [CO2]â¯×â¯UV interaction is changing along the vegetation season: an enhanced UV radiation stimulated a positive effect of elevated [CO2] on plant photosynthesis at the beginning of the vegetation season (short-term effect), whilst long-term cultivation reduced the stimulatory effect of elevated [CO2] (a clear down-regulation of photosynthesis). Down-regulation was, however, not found in plants grown under the conditions of excluded UV radiation. We found evidence that the down-regulation of photosynthesis is associated with a complex acclimation at different hierarchical and functional levels, including an acclimation of primary photochemical reactions, carboxylation activity of Rubisco enzyme, and stomatal conductance.
Asunto(s)
Dióxido de Carbono/farmacología , Fagus/fisiología , Fotosíntesis/efectos de los fármacos , Estaciones del Año , Rayos Ultravioleta , Clorofila/metabolismo , Fagus/efectos de los fármacos , Fagus/efectos de la radiación , Fluorescencia , Gases/metabolismo , Complejo de Proteína del Fotosistema II/metabolismo , Estomas de Plantas/efectos de los fármacos , Estomas de Plantas/fisiología , Estomas de Plantas/efectos de la radiación , Subunidades de Proteína/metabolismo , Ribulosa-Bifosfato Carboxilasa/metabolismoRESUMEN
Under the conditions of ongoing climate change, terrestrial ecosystems will be simultaneously exposed to a permanent rise in atmospheric CO2 concentration and increasing variability of such environmental factors as temperature, precipitation, and UV radiation. This will result in numerous interactions. The interactive effects caused by exposure to such multiple environmental factors are not yet well understood. We tested the hypotheses that enhanced UV radiation reduces the stimulatory effect of elevated CO2 concentration on plant biomass production and that it alters biomass allocation in broadleaved European beech (Fagus sylvatica L.) saplings. Our results after 2 years of exposure confirmed interactive effects of CO2 concentration and UV radiation on biomass production, and particularly on biomass allocation to roots and aboveground biomass. The strongest stimulatory effect of elevated CO2 on aboveground biomass and roots was found under ambient UV radiation, while both low and high UV doses reduced this stimulation. Nitrogen content in the roots and the distribution of nitrogen among leaves and roots were also significantly affected by interaction of CO2 concentration and UV radiation. The observed changes in leaf and root C:N stoichiometry were associated with altered morphological traits, and particularly with a change in the proportion of fine roots. As the biomass allocation and especially the proportion of fine roots can play an important role in effective water and nutrient use and acclimation to future climates, it is essential to obtain a deeper understanding of the links between C:N stoichiometry and biomass accumulation.
Asunto(s)
Biomasa , Dióxido de Carbono/farmacología , Carbono/análisis , Fagus/metabolismo , Nitrógeno/análisis , Rayos Ultravioleta , Fagus/efectos de los fármacos , Fagus/efectos de la radiación , Fotosíntesis/efectos de los fármacos , Fotosíntesis/efectos de la radiación , Hojas de la Planta/anatomía & histología , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/efectos de la radiación , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/metabolismo , Raíces de Plantas/efectos de la radiaciónRESUMEN
Forest functionality and productivity are directly related to canopy light interception and can be affected by potential damage from high irradiance. However, the mechanisms by which leaves adapt to the variable light environments along the multilayer canopy profile are still poorly known. We explored the leaf morphophysiological and metabolic responses to the natural light gradient in a pure European beech (Fagus sylvatica L.) forest at three different canopy heights (top, middle and bottom). Structural adjustment through light-dependent modifications in leaf mass per area was the reason for most of the variations in photosynthetic capacity. The different leaf morphology along the canopy influenced nitrogen (N) partitioning, water- and photosynthetic N-use efficiency, chlorophyll (Chl) fluorescence and quali-quantitative contents of photosynthetic pigments. The Chl a to Chl b ratio and the pool of xanthophyll-cycle pigments (VAZ) increased at the highest irradiance, as well as lutein and ß-carotene. The total pool of ascorbate and phenols was higher in leaves of the top and middle canopy layers when compared with the bottom layer, where the ascorbate peroxidase was relatively more activated. The non-photochemical quenching was strongly and positively related to the VAZ/(Chl aâ +â b) ratio, while Chl a/Chl b was related to the photochemical efficiency of photosystem II. Along the multilayer canopy profile, the high energy dissipation capacity of leaves was correlated to an elevated redox potential of antioxidants. The middle layer gave the most relevant contribution to leaf area index and carboxylation capacity of the canopy. In conclusion, a complex interplay among structural, physiological and biochemical traits drives the dynamic leaf acclimation to the natural gradients of variable light environments along the tree canopy profile. The relevant differences observed in leaf traits within the canopy positions of the beech forest should be considered for improving estimation of carbon fluxes in multilayer canopy models of temperate forests.
Asunto(s)
Fagus/fisiología , Hojas de la Planta/fisiología , Árboles/fisiología , Fagus/efectos de la radiación , Bosques , Italia , Luz , Fotosíntesis , Hojas de la Planta/anatomía & histología , Hojas de la Planta/efectos de la radiaciónRESUMEN
The purpose of this study was to obtain basic information on acclimation capacity of photosynthesis in Siebold's beech seedlings to increasing light intensity under future elevated CO2 conditions. We monitored leaf photosynthetic traits of these seedlings in changing light conditions (before removal of shade trees, the year after removal of shade trees and after acclimation to open conditions) in a 10-year free air CO2 enrichment experiment in northern Japan. Elevated CO2 did not affect photosynthetic traits such as leaf mass per area, nitrogen content and biochemical photosynthetic capacity of chloroplasts (i.e. maximum rate of carboxylation and maximum rate of electron transport) before removal of the shade trees and after acclimation to open conditions; in fact, a higher net photosynthetic rate was maintained under elevated CO2 . However, in the year after removal of the shade trees, there was no increase in photosynthesis rate under elevated CO2 conditions. This was not due to photoinhibition. In ambient CO2 conditions, leaf mass per area and nitrogen content were higher in the year after removal of shade trees than before, whereas there was no increase under elevated CO2 conditions. These results indicate that elevated CO2 delays the acclimation of photosynthetic traits of Siebold's beech seedlings to increasing light intensity.
Asunto(s)
Dióxido de Carbono/metabolismo , Fagus/fisiología , Fotosíntesis , Plantones/fisiología , Aclimatación , Transporte de Electrón , Fagus/efectos de los fármacos , Fagus/efectos de la radiación , Japón , Luz , Nitrógeno/análisis , Fenotipo , Fotosíntesis/efectos de los fármacos , Fotosíntesis/efectos de la radiación , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/fisiología , Hojas de la Planta/efectos de la radiación , Plantones/efectos de los fármacos , Plantones/efectos de la radiación , ÁrbolesRESUMEN
Spring phenology of temperate forest trees is optimized to maximize the length of the growing season while minimizing the risk of freezing damage. The release from winter dormancy is environmentally mediated by species-specific responses to temperature and photoperiod. We investigated the response of early spring phenology to temperature and photoperiod at different stages of dormancy release in cuttings from four temperate tree species in controlled environments. By tracking bud development, we were able to identify the onset of bud swelling and bud growth in Acer pseudoplatanus L., Fagus sylvatica L., Quercus petraea (Mattuschka) Liebl. and Picea abies (L.) H. Karst. At a given early stage of dormancy release, the onset and duration of the bud swelling prior to bud burst are driven by concurrent temperature and photoperiod, while the maximum growth rate is temperature dependent only, except for Fagus, where long photoperiods also increased bud growth rates. Similarly, the later bud burst was controlled by temperature and photoperiod (in the photoperiod sensitive species Fagus, Quercus and Picea). We conclude that photoperiod is involved in the release of dormancy during the ecodormancy phase and may influence bud burst in trees that have experienced sufficient chilling. This study explored and documented the early bud swelling period that precedes and defines later phenological stages such as canopy greening in conventional phenological works. It is the early bud growth resumption that needs to be understood in order to arrive at a causal interpretation and modelling of tree phenology at a large scale. Classic spring phenology events mark visible endpoints of a cascade of processes as evidenced here.
Asunto(s)
Fagus/fisiología , Picea/fisiología , Pinus/fisiología , Quercus/fisiología , Fagus/crecimiento & desarrollo , Fagus/efectos de la radiación , Luz , Fenotipo , Fotoperiodo , Picea/crecimiento & desarrollo , Picea/efectos de la radiación , Pinus/crecimiento & desarrollo , Pinus/efectos de la radiación , Quercus/crecimiento & desarrollo , Quercus/efectos de la radiación , Estaciones del Año , Especificidad de la Especie , Temperatura , Factores de Tiempo , ÁrbolesRESUMEN
Plant species use different strategies for maximizing growth and fitness under changing environmental conditions. At the ecosystem level, seedlings in particular compete with other vegetation components for light and nitrogen (N), which often constitute growth-limiting resources. In this study, we investigated the effect of light availability on the competition for N between seedlings of European beech and sycamore maple and analysed the consequences of this competition for the composition of N metabolites in fine roots. Our results show different strategies in N acquisition between beech and sycamore maple. Both species responded to reduced light availability by adapting their morphological and physiological traits with a decrease in biomass and net assimilation rate and an increase in specific leaf area and leaf area ratio. For beech seedlings, competition with sycamore maple led to a reduction in organic N uptake capacity. Reduced light availability led to a decrease in ammonium, but an increase in glutamine-N uptake capacity in sycamore maple. However, this response was stronger compared with that of beech and was accompanied by reduced growth. Thus, our results suggest better adaptation of N acquisition to reduced light availability in beech compared with sycamore maple seedlings.
Asunto(s)
Acer/metabolismo , Adaptación Fisiológica , Fagus/metabolismo , Nitrógeno/metabolismo , Acer/crecimiento & desarrollo , Acer/efectos de la radiación , Compuestos de Amonio/metabolismo , Biomasa , Isótopos de Carbono/análisis , Fagus/crecimiento & desarrollo , Fagus/efectos de la radiación , Glutamina/metabolismo , Luz , Nitratos/metabolismo , Isótopos de Nitrógeno/análisis , Hojas de la Planta/crecimiento & desarrollo , Hojas de la Planta/metabolismo , Hojas de la Planta/efectos de la radiación , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Raíces de Plantas/efectos de la radiación , Plantones/crecimiento & desarrollo , Plantones/metabolismo , Plantones/efectos de la radiación , Suelo , ÁrbolesRESUMEN
The use of the photochemical reflectance index (PRI) as a promising proxy of light use efficiency (LUE) has been extensively studied, and some issues have been identified, notably the sensitivity of PRI to leaf pigment composition and the variability in PRI response to LUE because of stress. In this study, we introduce a method that enables us to track the short-term PRI response to LUE changes because of photosynthetically active radiation (PAR) changes. The analysis of these short-term relationships between PRI and LUE throughout the growing season in two species (Quercus robur L. and Fagus sylvatica L.) under two different soil water statuses showed a clear change in PRI response to LUE, which is related to leaf pigment content. The use of an estimated or approximated PRI0, defined as the PRI of perfectly dark-adapted leaves, allowed us to separate the PRI variability due to leaf pigment content changes and the physiologically related PRI variability over both daily (PAR-related) and seasonal (soil water content-related) scales. The corrected PRI obtained by subtracting PRI0 from the PRI measurements showed a good correlation with the LUE over both of the species, soil water statuses and over the entire growing season.
Asunto(s)
Fagus/fisiología , Fotosíntesis , Quercus/fisiología , Carbono/metabolismo , Clorofila/metabolismo , Sequías , Fagus/efectos de la radiación , Fluorescencia , Fotoquímica , Hojas de la Planta/metabolismo , Hojas de la Planta/fisiología , Quercus/efectos de la radiación , Agua/metabolismoRESUMEN
BACKGROUND AND AIMS: The coexistence of forest tree species has often been linked to differences among species in terms of their response to light availability during the regeneration stage. From this perspective, species coexistence results from growth-growth or mortality-growth trade-offs along spatial light gradients. Experimental evidence of growth-growth trade-offs in natural conditions is sparse due to various confounding factors that potentially hinder the relationship. This study examined growth hierarchies along light gradients between two tree species with contrasting shade tolerance by controlling potential confounding factors such as seedling size, seedling status, seedling density and species composition. METHODS: Natural regenerated shade-tolerant Fagus sylvatica and shade-intermediate Quercus petraea seedlings were used, and growth rankings over a 4-year period were compared in 8- to 10-year-old tree seedlings. KEY RESULTS: No rank reversal occurs between the two species along the light gradient, or along the density, mixture or seedling size gradients. The shade-tolerant species was always the more competitive of the two. Pronounced effects of initial size on seedling growth were observed, whereas the effects of light and competition by neighbours were of secondary importance. The paramount effect of size, which results from the asymmetric nature of interseedling competition, gives a strong advantage to tall seedlings over the long term. CONCLUSIONS: This study extends previous efforts to identify potential drivers of rank reversals in young tree mixtures. It does not support the classical assumption that spatial heterogeneity in canopy opening explains the coexistence of the two species studied. It suggests that spatial variation in local size hierarchies among seedlings that may be caused by seedling emergence time or seedling initial performance is the main driver of the dynamics of these mixed stands.
Asunto(s)
Adaptación Fisiológica/efectos de la radiación , Fagus/fisiología , Luz , Quercus/fisiología , Plantones/fisiología , Plantones/efectos de la radiación , Simbiosis/efectos de la radiación , Fagus/anatomía & histología , Fagus/crecimiento & desarrollo , Fagus/efectos de la radiación , Francia , Quercus/anatomía & histología , Quercus/crecimiento & desarrollo , Quercus/efectos de la radiación , Regeneración/efectos de la radiaciónRESUMEN
The possibility of photochemical improvement of color stability by UV-irradiation treatment at coating-wood biointerfaces was investigated. The surface of beech wood was partially delignified by exposure to UV-irradiation by passing of samples under UV lamps: a mercury (Hg) lamp with a peak wavelength of 366nm, a gallium (Ga) lamp with peak wavelengths at 410 and 420nm and a combination of a mercury and a gallium (Hg+Ga) lamp. After UV-irradiation, the samples were coated with a UV-curable acrylic coating. The number of passes under the lamps was varied to determine the optimum combination of the number of passes and the lamp type that produces the smallest change in color of the coated wood in an indoor environment. The coated samples that had been UV-irradiated by passing 15 times under both an Hg lamp and a Ga lamp showed the smallest change in color (decrease in the ΔE by 23.23% compared to the untreated coated samples) after 72h of accelerated artificial sunlight exposure. Microscope images of the same samples showed deformation of the cells in wood surface layer and a roughening of the ?wood-coating line", which could be related to a partial removal of lignin. These findings suggest that delignification of wood leads to more photo-stable polymer-wood interfaces in terms of color.
Asunto(s)
Acrilatos/química , Fagus/efectos de la radiación , Lignina/química , Madera/efectos de la radiación , Color , Fagus/química , Fotólisis , Temperatura , Factores de Tiempo , Rayos Ultravioleta , Madera/químicaRESUMEN
Using an infrared camera, we measured the leaf temperature across different canopy positions of a 23-m-tall deciduous forest tree (Fagus sylvatica L.) including typical sun and shade leaves as well as intermediate leaf forms, which differed significantly in specific leaf area (SLA). We calculated a temperature index (I(G)) and a crop water stress index (CWSI) using the surface temperatures of wet and dry reference leaves. Additional indices were computed using air temperature plus 5 °C (I(G) + 5, CWSI + 5) as dry references. The minimum temperature of the wet leaf and the maximum temperature of the dry leaf proved to be most suitable as reference values. We correlated the temperature indices with leaf area-related conductance to water vapor (g(L)) using porometry at the leaf level and using xylem sap flow at the branch level. At the leaf and at the branch level, I(G) and CWSI were equally well suited as proxies of g(L), whereas the relationships of I(G) + 5 and CWSI + 5 with g(L) were only weak or even insignificant. At the leaf level, the correlations of I(G) and CWSI with g(L) were significant in all parts of the crown. The slopes of g(L) vs. I(G) and CWSI did not differ significantly among the crown parts; this indicates that they were not influenced by SLA or irradiance. At the branch level, close correlations (r > 0.8) were found between temperature indices and g(L) across the crown. These results demonstrate that satisfactory relationships between temperature indices and g(L) can be established in tall trees even in those canopy parts that are exposed to relatively low levels of irradiance and exhibit relatively low values of g(L).
Asunto(s)
Fagus/fisiología , Transpiración de Plantas/fisiología , Termografía/métodos , Deshidratación , Fagus/efectos de la radiación , Alemania , Humedad , Luz , Hojas de la Planta/fisiología , Hojas de la Planta/efectos de la radiación , Transpiración de Plantas/efectos de la radiación , Temperatura , Termografía/instrumentación , Árboles/fisiología , Árboles/efectos de la radiación , Agua/fisiología , Viento , Xilema/fisiología , Xilema/efectos de la radiaciónRESUMEN
BACKGROUND AND AIMS: Forest tree saplings that grow in the understorey undergo frequent changes in their light environment to which they must adapt to ensure their survival and growth. Crown architecture, which plays a critical role in light capture and mechanical stability, is a major component of sapling adaptation to canopy disturbance. Shade-adapted saplings typically have plagiotropic stems and branches. After canopy opening, they need to develop more erect shoots in order to exploit the new light conditions. The objective of this study was to test whether changes in sapling stem inclination occur after canopy opening, and to analyse the morphological changes associated with stem reorientation. METHODS: A 4-year canopy-opening field experiment with naturally regenerated Fagus sylvatica and Acer pseudoplatanus saplings was conducted. The appearance of new stem axes, stem basal diameter and inclination along the stem were recorded every year after canopy opening. KEY RESULTS: Both species showed considerable stem reorientation resulting primarily from uprighting (more erect) shoot movements in Fagus, and from uprighting movements, shoot elongation and formation of relay shoots in Acer. In both species, the magnitude of shoot uprighting movements was primarily related to initial stem inclination. Both the basal part and the apical part of the stem contributed to uprighting movements. Stem movements did not appear to be limited by stem size or by stem growth. CONCLUSIONS: Stem uprighting movements in shade-adapted Fagus and Acer saplings following canopy disturbance were considerable and rapid, suggesting that stem reorientation processes play a significant role in the growth strategy of the species.
Asunto(s)
Acer/crecimiento & desarrollo , Acer/efectos de la radiación , Adaptación Fisiológica/fisiología , Fagus/crecimiento & desarrollo , Fagus/efectos de la radiación , Acer/anatomía & histología , Acer/metabolismo , Fagus/anatomía & histología , Fagus/metabolismo , Francia , Gravitropismo/fisiología , Luz , Modelos Biológicos , Hojas de la Planta/anatomía & histología , Hojas de la Planta/crecimiento & desarrollo , Hojas de la Planta/metabolismo , Hojas de la Planta/efectos de la radiación , Brotes de la Planta/anatomía & histología , Brotes de la Planta/crecimiento & desarrollo , Brotes de la Planta/metabolismo , Brotes de la Planta/efectos de la radiación , Árboles/anatomía & histología , Árboles/crecimiento & desarrollo , Árboles/metabolismo , Árboles/efectos de la radiaciónRESUMEN
Xylem vulnerability to cavitation is a key parameter in understanding drought resistance of trees. We determined the xylem water pressure causing 50% loss of hydraulic conductivity (P(50)), a proxy of vulnerability to cavitation, and we evaluated the variability of this trait at tree and population levels for Fagus sylvatica. We checked for the effects of light on vulnerability to cavitation of stem segments together with a time series variation of P(50). Full sunlight-exposed stem segments were less vulnerable to cavitation than shade-exposed ones. We found no clear seasonal change of P(50), suggesting that this trait was designed for a restricted period. P(50) varied for populations settled along a latitudinal gradient, but not for those sampled along an altitudinal gradient. Moreover, mountainside exposure seemed to play a major role in the vulnerability to cavitation of beech populations, as we observed the differences along north-facing sides but not on south-facing sides. Unexpectedly, both north-facing mountainside and northern populations appeared less vulnerable than those grown on the southern mountainside or in the South of France. These results on beech populations were discussed with respect to the results at within-tree level.
Asunto(s)
Sequías , Ecosistema , Fagus/metabolismo , Agua/metabolismo , Xilema/metabolismo , Adaptación Fisiológica , Altitud , Fagus/efectos de la radiación , Francia , Fenotipo , Presión , Estaciones del Año , Luz Solar , Xilema/efectos de la radiaciónRESUMEN
In this study, the effects of different light intensities either in direct sunlight or in the shade crown of adult beech (Fagus sylvatica L.) trees on delta13C and Delta18O were determined under ambient (1 x O3) and twice-ambient (2 x O3) atmospheric ozone concentrations during two consecutive years (2003 and 2004). We analysed the isotopic composition in leaf bulk, leaf cellulose, phloem and xylem material and related the results to (a) meteorological data (air temperature, T and relative humidity, RH), (b) leaf gas exchange measurements (stomatal conductance, g(s); transpiration rate, E; and maximum photosynthetic activity, A(max)) and (c) the outcome of a steady-state evaporative enrichment model. Delta13C was significantly lower in the shade than in the sun crown in all plant materials, whilst Delta18O was increased significantly in the shade than in the sun crown in bulk material and cellulose. Elevated ozone had no effect on delta13C, although Delta18O was influenced by ozone to varied degrees during single months. We observed significant seasonal changes for both parameters, especially in 2004, and also significant differences between the study years. Relating the findings to meteorological data and gas exchange parameters, we conclude that the differences in Delta18O between the sun and the shade crown were predominantly caused by the Péclet effect. This assumption was supported by the modelled Delta18O values for leaf cellulose. It was demonstrated that independent of RH, light-dependent reduction of stomatal conductance (and thus transpiration) and of A(max) can drive the pattern of Delta18O increase with the concomitant decrease of delta13C in the shade crown. The effect of doubling ozone levels on time-integrated stomatal conductance and transpiration as indicated by the combined analysis of Delta18O and delta13C was much lower than the influence caused by the light exposure.
Asunto(s)
Carbono/metabolismo , Fagus/metabolismo , Oxígeno/metabolismo , Ozono/farmacología , Isótopos de Carbono , Celulosa/metabolismo , Fagus/efectos de los fármacos , Fagus/efectos de la radiación , Humedad , Isótopos de Oxígeno , Floema/efectos de los fármacos , Floema/metabolismo , Floema/efectos de la radiación , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/metabolismo , Hojas de la Planta/efectos de la radiación , Estomas de Plantas/efectos de los fármacos , Estomas de Plantas/metabolismo , Estomas de Plantas/fisiología , Estaciones del Año , Luz Solar , Temperatura , Xilema/efectos de los fármacos , Xilema/metabolismo , Xilema/efectos de la radiaciónRESUMEN
Leaf photosynthesis is known to acclimate to the actual irradiance received by the different layers of a canopy. This acclimation is usually described in terms of changes in leaf structure, and in photosynthetic capacity. Photosynthetic capacity is likely to be affected by mesophyll conductance to CO(2) which has seldom been assessed in tree species, and whose plasticity in response to local irradiance is still poorly known. Structural [N and chlorophyll content, leaf mass to area ratio (LMA)] and functional leaf traits [maximum carboxylation rate (V(cmax)), maximum light-driven electron flux (J(max)), and mesophyll conductance (g(i))] were assessed by measuring leaf response curves of net CO(2) assimilation versus intercellular CO(2) partial pressure, along a vertical profile across a beech canopy, and by fitting a version of the Farquhar model including g(i). The measurements were repeated five times during a growth season to catch potential seasonal variation. Irradiance gradients resulted in large decreasing gradients of LMA, g(i), V(cmax), and J(max). Relative allocation of leaf N to the different photosynthetic processes was only slightly affected by local irradiance. Seasonal changes after leaf expansion and before induction of leaf senescence were only minor. Structural equation modelling confirmed that LMA was the main driving force for changes in photosynthetic traits, with only a minor contribution of leaf Nitrogen content. In conclusion, mesophyll conductance to CO(2) displays a large plasticity that scales with photosynthetic capacity across a tree canopy, and that it is only moderately (if at all) affected by seasonal changes in the absence of significant soil water depletion.
Asunto(s)
Dióxido de Carbono/metabolismo , Fagus/metabolismo , Fotosíntesis , Hojas de la Planta/metabolismo , Estaciones del Año , Fagus/química , Fagus/efectos de la radiación , Fotosíntesis/efectos de la radiación , Hojas de la Planta/química , Hojas de la Planta/efectos de la radiaciónRESUMEN
Rare gregarious flowering of understorey bamboo species occurs in temperate and subtropical forests around the world, but the ecological consequences of this phenomenon for forest regeneration are not well understood.Field experiments were conducted in an old-growth temperate forest in Patagonia,Argentina after a massive bamboo flowering event, to examine whether light quality and other changes in microhabitats could affect seed germination and growth of overstorey species. Germination of southern beech (Nothofagus obliqua) was positively correlated with red:far red (R:FR) ratios in a range of microhabitats generated by the death of the understorey bamboo (Chusquea culeou). Experimental modification of understorey R:FR ratios to mimic alternative light environments reversed this germination response in plots with senescent understorey, but not in plots with live bamboo. Laboratory incubations demonstrated a significant interaction between R:FR ratios and thermal amplitude in promoting seed germination. Microhabitats also significantly affected the growth of emerged seedlings. Microenvironmental changes generated by this flowering event appear to have opened a window of opportunity for germination and growth of overstorey species.We demonstrate that natural gradients in light quality associated with this ecological phenomenon are a major component affecting forest regeneration in this ecosystem.
Asunto(s)
Luz , Sasa/fisiología , Argentina , Ecosistema , Fagus/crecimiento & desarrollo , Fagus/fisiología , Fagus/efectos de la radiación , Flores/crecimiento & desarrollo , Flores/fisiología , Germinación , Regeneración , Sasa/crecimiento & desarrollo , Sasa/efectos de la radiaciónRESUMEN
The effects of elevated O3 on photosynthetic properties in adult beech trees (Fagus sylvatica) were investigated in relation to leaf mass per area as a measure of the gradually changing, within-canopy light availability. Leaves under elevated O3 showed decreased stomatal conductance at unchanged carboxylation capacity of Rubisco, which was consistent with enhanced delta 13C of leaf organic matter, regardless of the light environment during growth. In parallel, increased energy demand for O3 detoxification and repair was suggested under elevated O3 owing to enhanced dark respiration. Only in shade-grown leaves,light-limited photosynthesis was reduced under elevated O3, this effect being accompanied by lowered F(v)/F(m). These results suggest that chronic O3 exposure primarily caused stomatal closure to adult beech trees in the field regardless of the within-canopy light gradient. However, light limitation apparently raised the O3 sensitivity of photosynthesis and accelerated senescence in shade leaves.
Asunto(s)
Contaminantes Atmosféricos/farmacología , Fagus/efectos de los fármacos , Luz , Ozono/farmacología , Fotosíntesis/efectos de los fármacos , Biomasa , Respiración de la Célula/efectos de los fármacos , Fagus/fisiología , Fagus/efectos de la radiación , Nitrógeno/metabolismo , Oxidantes Fotoquímicos/farmacología , Hojas de la Planta/anatomía & histología , Hojas de la Planta/efectos de los fármacos , Estomas de Plantas/efectos de los fármacos , Lluvia , Estaciones del AñoRESUMEN
We used height growth data from a 7-year field experiment with European beech (Fagus sylvatica L.) seedlings to test the hypothesis that the effects of above- and belowground resources on height growth depend on seedling size and age. Photosynthetically active radiation (PAR) was determined by hemispheric photography, and estimates of fine root biomass of the overstory trees were used as an inverse proportional surrogate for belowground resource availability. For recently germinated seedlings growing under the canopy of Picea abies (L.) Karst., belowground resource availability affected height growth more than light. During subsequent stages of seedling development, apart from initial seedling size, PAR increasingly determined seedling growth. Besides initial size, seedling age determined the effects of above- and belowground resources on seedling height growth. In seedlings identical in initial size but differing in age, the increase in height growth with increasing PAR was greater in older seedlings than in younger seedlings. The ranking of seedling height by year showed that small differences in size at the end of the first growing season resulted in continuously increasing differences during the following years. Mortality data indicated that the chances of a seedling surviving intraspecific competition was strongly determined by its dominance ranking within the first 5 years after establishment.
Asunto(s)
Fagus/efectos de la radiación , Luz , Plantones/efectos de la radiación , Ecosistema , Fagus/crecimiento & desarrollo , Fotosíntesis/efectos de la radiación , Plantones/crecimiento & desarrollo , Suelo/análisisRESUMEN
Two ecotypes of Japanese beech (Fagus crenata Blume), the Pacific Ocean type (PAO) and the Japan Sea type (JAS), show different responses to high solar irradiance. When PAO and JAS saplings were grown in continuous high-light (H), leaves of JAS became pale green. To elucidate this phenomenon, we investigated in vivo photochemistry based on pigment concentrations of Photosystem (PS) I and PS II and Western blot analysis. In JAS-H leaves, the amount of D1-protein decreased, resulting in decreases in the maximal quantum yield of PS II (F(v)/F(m)) and electron transport rate, whereas PAO-H leaves maintained high activities. The PS I photochemistry determined by measurement of P-700 photo-oxidation showed that the intersystem electron pool size was 1.4 times greater in JAS-H leaves than in PAO-H leaves. Furthermore, the re-reduction kinetics of P-700(+) showed that cyclic electron transport around PS I was 1.2 times faster in PAO-H leaves than in JAS-H leaves. Analysis of the area over the fluorescence induction kinetics indicated that the relative abundance of the PS IIalpha center increased in PAO-H leaves, whereas JAS leaves were observed to have low acclimation capacity to high light. These results demonstrate that PAO leaves possess acclimation mechanisms to continuous high light, whereas JAS leaves are more vulnerable to continuous high light, resulting in reduced leaf longevity owing to photoinhibition caused by increases in the intersystem electron pool size and suppression of photochemistry at the level of PS I and PS II.
Asunto(s)
Fagus/fisiología , Luz , Complejo de Proteína del Fotosistema I/metabolismo , Complejo de Proteína del Fotosistema II/metabolismo , Western Blotting , Clorofila/metabolismo , Clorofila A , Cromatografía Líquida de Alta Presión , Electroforesis en Gel de Poliacrilamida , Fagus/metabolismo , Fagus/efectos de la radiación , Japón , Océanos y Mares , Océano PacíficoRESUMEN
Knowledge of responses of photosynthesis, respiration, and stomatal conductance to cumulative ozone uptake (COU) is still scarce, and this is particularly the case for adult trees. The effect of ozone (O(3)) exposure on trees was examined with 60-year-old beech trees (FAGUS SYLVATICA) at a forest site of southern Germany. Trees were exposed to the ambient O(3) regime (1 x O(3)) or an experimentally elevated twice-ambient O(3) regime (2 x O(3)). The elevated 2 x O (3) regime was provided by means of a free-air O(3) canopy exposure system. The hypotheses were tested that (1) gas exchange is negatively affected by O(3) and (2) the effects of O(3) are dose-dependent and thus the sizes of differences between treatments are positively related to COU. Gas exchange (light-saturated CO(2) uptake rate A(max), stomatal conductance g (s), maximum rate of carboxylation Vc (max), ribulose-1,5-bisphosphate turnover limited rate of photosynthesis J (max), CO(2) compensation point CP, apparent quantum yield of net CO(2) uptake AQ, carboxylation efficiency CE, day- and nighttime respiration) and chlorophyll fluorescence (electron transfer rate, ETR) were measured IN SITU on attached sun and shade leaves. Measurements were made periodically throughout the growing seasons of 2003 (an exceptionally dry year) and 2004 (a year with average rainfall). In 2004 Vc(max), J(max), and CE were lower in trees receiving 2 x O(3) compared with the ambient O(3) regime (1 x O(3)). Treatment differences in Vc (max), J (max), CE were rather small in 2004 (i.e., parameter levels were lower by 10 - 30 % in 2 x O(3) than 1 x O(3)) and not significant in 2003. In 2004 COU was positively correlated with the difference between treatments in A (max), g (s), and ETR (i.e., consistent with the dose-dependence of O(3)'s deleterious effects). However, in 2003, differences in A(max), g (s), and ETR between the two O(3) regimes were smaller at the end of the dry summer 2003 (i.e., when COU was greatest). The relationship of COU with effects on gas exchange can apparently be complex and, in fact, varied between years and within the growing season. In addition, high doses of O(3) did not always have significant effects on leaf gas exchange. In view of the key findings, both hypotheses were to be rejected.
Asunto(s)
Fagus/efectos de los fármacos , Fagus/fisiología , Ozono/farmacología , Fotosíntesis/efectos de los fármacos , Dióxido de Carbono/metabolismo , Respiración de la Célula/efectos de los fármacos , Respiración de la Célula/efectos de la radiación , Desastres , Transporte de Electrón/efectos de los fármacos , Transporte de Electrón/efectos de la radiación , Fagus/efectos de la radiación , Luz , Fotosíntesis/efectos de la radiación , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/efectos de la radiación , Lluvia , TemperaturaRESUMEN
In this study the influence of chronic free-air ozone exposure and of different meteorological conditions in the very dry year 2003 and the more humid year 2004 on the antioxidative system in sun and shade leaves of adult FAGUS SYLVATICA trees were investigated. Contents of ascorbate, glutathione, and alpha-tocopherol, as well as chloroplast pigments were determined under ambient (1 x O(3)) and double ambient (2 x O(3)) ozone concentrations. Ozone affected the antioxidative system in June and July, causing lower ascorbate contents in the apoplastic space, a more oxidized redox state of ascorbate and glutathione and an increase in pigment contents predominantly in the shade crown. For all measured parameters significant differences between the years were observed. In 2004 the redox state of ascorbate and glutathione was in a more reduced state and leaf contents of alpha-tocopherol, pigments of the xanthophyll cycle, beta-carotene, lutein, neoxanthin, and alpha-carotene were lower compared to 2003. Contents of total glutathione and chlorophyll a + b were increased in the second year. These results indicate a strong influence of the drought conditions in 2003 on the antioxidative system of beech overruling the ozone effects. Shade leaves showed lower contents of ascorbate in both years and the redox states of ascorbate and glutathione were more oxidized compared to sun leaves. Contents of photoprotective and accessory pigments generally were enhanced and the de-epoxidation state of the xanthophyll cycle was lower in the shade compared to the sun crown. Exhibiting less antioxidants shade leaves seem to be more sensitive against ozone than sun leaves.