RESUMEN
Transplanting metabolic reactions from one species into another has many uses as a research tool with applications ranging from optogenetics to crop production. Ferredoxin (Fd), the enzyme that most often supplies electrons to these reactions, is often overlooked when transplanting enzymes from one species to another because most cells already contain endogenous Fd. However, we have shown that the production of chromophores used in Phytochrome B (PhyB) optogenetics is greatly enhanced in mammalian cells by expressing bacterial and plant Fds with ferredoxin-NADP+ reductases (FNR). We delineated the rate limiting factors and found that the main metabolic precursor, heme, was not the primary limiting factor for producing either the cyanobacterial or plant chromophores, phycocyanobilin or phytochromobilin, respectively. In fact, Fd is limiting, followed by Fd+FNR and finally heme. Using these findings, we optimized the PCB production system and combined it with a tissue penetrating red/far-red sensing PhyB optogenetic gene switch in animal cells. We further characterized this system in several mammalian cell lines using red and far-red light. Importantly, we found that the light-switchable gene system remains active for several hours upon illumination, even with a short light pulse, and requires very small amounts of light for maximal activation. Boosting chromophore production by matching metabolic pathways with specific ferredoxin systems will enable the unparalleled use of the many PhyB optogenetic tools and has broader implications for optimizing synthetic metabolic pathways.
Asunto(s)
Proteínas de Arabidopsis , Proteínas Bacterianas , Ferredoxinas , Optogenética , Fitocromo B , Sulfito Reductasa (Ferredoxina) , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/biosíntesis , Proteínas de Arabidopsis/genética , Proteínas Bacterianas/biosíntesis , Proteínas Bacterianas/genética , Ferredoxinas/biosíntesis , Ferredoxinas/genética , Células HEK293 , Humanos , Fitocromo B/biosíntesis , Fitocromo B/genética , Sulfito Reductasa (Ferredoxina)/biosíntesis , Sulfito Reductasa (Ferredoxina)/genética , Synechococcus/genética , Synechococcus/metabolismoRESUMEN
The unicellular green alga Chlamydomonas reinhardtii reacts to sulfur (S) starvation with the increased expression of numerous genes. One gene which is induced in illuminated anaerobic S-deprived cells is the ferredoxin-5 gene (FDX5). To test FDX5 transcriptional regulation in aerobic cultures, we used a real-time PCR analysis and an artificial microRNA approach. We demonstrated that FDX5 gene is controlled by S deprivation independently of anoxia-treatment. The Ser/Thr kinase SNRK2.1 is necessary for expression of FDX5 during deprivation to S. Copper response regulator 1 (CRR1) is not involved in FDX5 up-regulation in S-deficient cells under aerobic conditions. Furthermore, expression of FDX5 is negatively regulated by nitric oxide (NO). Moreover, truncated hemoglobin 1 (THB1) underexpression resulted in the decrease in FDX5 transcript abundance in S-deficient cells under aerobic conditions. Together, our results imply that the FDX5 gene is controlled by NO in THB1-dependent pathway under conditions of depleted S supply.
Asunto(s)
Chlamydomonas reinhardtii/metabolismo , Ferredoxinas/biosíntesis , Regulación de la Expresión Génica de las Plantas , Proteínas de Plantas/biosíntesis , Azufre/deficiencia , AerobiosisRESUMEN
Optogenetics is a powerful tool to precisely manipulate cell signaling in space and time. For example, protein activity can be regulated by several light-induced dimerization (LID) systems. Among them, the phytochrome B (PhyB)-phytochrome-interacting factor (PIF) system is the only available LID system controlled by red and far-red lights. However, the PhyB-PIF system requires phycocyanobilin (PCB) or phytochromobilin as a chromophore, which must be artificially added to mammalian cells. Here, we report an expression vector that coexpresses HO1 and PcyA with Ferredoxin and Ferredoxin-NADP+ reductase for the efficient synthesis of PCB in the mitochondria of mammalian cells. An even higher intracellular PCB concentration was achieved by the depletion of biliverdin reductase A, which degrades PCB. The PCB synthesis and PhyB-PIF systems allowed us to optogenetically regulate intracellular signaling without any external supply of chromophores. Thus, we have provided a practical method for developing a fully genetically encoded PhyB-PIF system, which paves the way for its application to a living animal.
Asunto(s)
Ferredoxina-NADP Reductasa/biosíntesis , Ferredoxinas/biosíntesis , Hemo Oxigenasa (Desciclizante)/biosíntesis , Optogenética , Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH/genética , Oxidorreductasas/biosíntesis , Ficobilinas/biosíntesis , Ficocianina/biosíntesis , Línea Celular Tumoral , Vectores Genéticos/genética , Células HeLa , Humanos , Luz , Ficobilinas/genética , Ficocianina/genética , Transducción de Señal/genéticaRESUMEN
Ferredoxins are small, acidic proteins containing iron-sulfur clusters that are widespread in living organisms. They play key roles as electron carriers in various metabolic processes, including respiration, photosynthesis, fermentation, nitrogen fixation, carbon dioxide fixation, and hydrogen production. However, only several kinds of ferredoxins are commercially available now, greatly limiting the investigation of ferredoxin-related enzymes and metabolic processes. Here we describe the heterologous overproduction of 2[4Fe4S]- and [2Fe2S]-type clostridial ferredoxins and [2Fe2S]-type agrobacterial ferredoxin. Adding extra iron and sulfur sources to the medium in combination with using Escherichia coli C41(DE3) harboring pCodonplus and pRKISC plasmids as host greatly enhanced iron-sulfur cluster synthesis in the three ferredoxins. After induction for 12 h in terrific broth and purification by affinity chromatography and anion exchange chromatography, approximately 3.4 mg of streptavidin (Strep)-tagged and 3.7 mg of polyhistidine (His)-tagged clostridial 2[4Fe4S] ferredoxins were obtained from 1 l of culture. Excitingly, after induction for 24 h in terrific broth, around 40 mg of His-tagged clostridial [2Fe2S] and 23 mg of His-tagged agrobacterial [2Fe2S] ferredoxins were purified from 1 l of culture. The recombinant ferredoxins apparently exhibited identical properties and physiological function to native ferredoxins. No negative impact of two different affinity tags on ferredoxin activity was found. In conclusion, we successfully developed a convenient method for heterologous overproduction of the three kinds of ferredoxins with satisfactory yields and activities, which would be very helpful for the ferredoxin-related researches.
Asunto(s)
Ferredoxinas/biosíntesis , Proteínas Hierro-Azufre/biosíntesis , Proteínas Recombinantes/biosíntesis , Agrobacterium/genética , Cromatografía de Afinidad , Clostridium/genética , Espectroscopía de Resonancia por Spin del Electrón , Escherichia coli/genética , Ferredoxinas/química , Ferredoxinas/aislamiento & purificación , Hierro/química , Proteínas Hierro-Azufre/química , Proteínas Hierro-Azufre/aislamiento & purificación , Plásmidos/biosíntesis , Plásmidos/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/aislamiento & purificaciónRESUMEN
Sulfate adenylyltransferase gene and 4Fe-4S ferredoxin gene are the key genes related to sulfur and iron oxidations during bioleaching system, respectively. In order to better understand the bioleaching and microorganism synergistic mechanism in chalcopyrite bioleaching by mixed culture of moderate thermophiles, expressions of the two energy metabolism genes and community dynamics of free and attached microorganisms were investigated. Specific primers were designed for real-time quantitative PCR to study the expression of these genes. Real-time PCR results showed that sulfate adenylyltransferase gene was more highly expressed in Sulfobacillus thermosulfidooxidans than that in Acidithiobacillus caldus, and expression of 4Fe-4S ferredoxin gene was higher in Ferroplasma thermophilum than that in S. thermosulfidooxidans and Leptospirillum ferriphilum. The results indicated that in the bioleaching system of chalcopyrite concentrate, sulfur and iron oxidations were mainly performed by S. thermosulfidooxidans and F. thermophilum, respectively. The community dynamics results revealed that S. thermosulfidooxidans took up the largest proportion during the whole period, followed by F. thermophilum, A. caldus, and L. ferriphilum. The CCA analysis showed that 4Fe-4S ferredoxin gene expression was mainly affected (positively correlated) by high pH and elevated concentration of ferrous ion, while no factor was observed to prominently influence the expression of sulfate adenylyltransferase gene.
Asunto(s)
Biota , Cobre/metabolismo , Ferredoxinas/genética , Perfilación de la Expresión Génica , Microbiología del Suelo , Sulfato Adenililtransferasa/genética , Ferredoxinas/biosíntesis , Concentración de Iones de Hidrógeno , Hierro/metabolismo , Oxidación-Reducción , Reacción en Cadena en Tiempo Real de la Polimerasa , Sulfato Adenililtransferasa/biosíntesis , Azufre/metabolismoRESUMEN
Ferredoxin 1 (FDX1; adrenodoxin) is an iron-sulfur protein that is involved in various metabolic processes, including steroid hormone synthesis in mammalian tissues. We investigated the transcriptional regulation of FDX1 in ovarian granulosa cells. Previously, we reported that the NR5A family, including steroidogenic factor-1 (SF-1) and liver receptor homolog-1 could induce differentiation of human mesenchymal stem cells (hMSCs) into steroidogenic cells. A ChIP assay showed that SF-1 could bind to the FDX1 promoter in differentiated hMSCs. Luciferase reporter assays showed that transcription of FDX1 was synergistically activated by the NR5A family and 8Br-cAMP treatment through two SF-1 binding sites and a CRE-like sequence in a human ovarian granulosa cell line, KGN. Knockdown of FDX1 attenuated progesterone production in KGN cells. These results indicate transcription of FDX1 is regulated by the NR5A family and cAMP signaling, and participates in steroid hormone production in ovarian granulosa cells.
Asunto(s)
Ferredoxinas/genética , Células de la Granulosa/metabolismo , Receptores Citoplasmáticos y Nucleares/metabolismo , Factor Esteroidogénico 1/metabolismo , 8-Bromo Monofosfato de Adenosina Cíclica/farmacología , Adrenodoxina/genética , Animales , Diferenciación Celular , Línea Celular Tumoral , AMP Cíclico/metabolismo , Proteínas de Unión al ADN/metabolismo , Femenino , Ferredoxinas/biosíntesis , Regulación de la Expresión Génica , Células HeLa , Humanos , Células Madre Mesenquimatosas/metabolismo , Progesterona/biosíntesis , Regiones Promotoras Genéticas , Unión Proteica , Interferencia de ARN , ARN Interferente Pequeño , Ratas , Ratas Wistar , Transducción de Señal , Factor Esteroidogénico 1/efectos de los fármacos , Transcripción GenéticaRESUMEN
Heterometallic [AgFe(3)S(4)] iron-sulfur clusters assembled in wild-type Pyrococcus furiosus ferredoxin and two variants, D14C and D14H, are characterized. The crystal structure of the [AgFe(3)S(4)] D14C variant shows that the silver(I) ion is indeed part of the cluster and is coordinated to the thiolate group of residue 14. Cyclic voltammetry shows one redox pair with a reduction potential of +220 mV versus the standard hydrogen electrode which is assigned to the [AgFe(3)S(4)](2+/+) couple. The oxidized form of the [AgFe(3)S(4)] D14C variant is stable in the presence of dioxygen, whereas the oxidized forms of the [AgFe(3)S(4)] wild type and D14H variants convert to the [Fe(3)S(4)] ferredoxin form. The monovalent d (10) silver(I) ion stabilizes the [Fe(3)S(4)](+/0) cluster fragment, as opposed to divalent d (10) metal ions, resulting in more than 0.4 V difference in reduction potentials between the silver(I) and, e.g., zinc(II) heterometallic [MFe(3)S(4)] ferredoxins. The trend in reduction potentials for the variants containing the [AgFe(3)S(4)] cluster is wild type ≤ D14C < D14H and shows the same trend as reported for the variants containing the [Fe(3)S(4)] cluster, but is different from the D14C < D14H < wild type trend reported for the [Fe(4)S(4)] ferredoxin. The similarity in the reduction potential trend for the variants containing the heterometallic [AgFe(3)S(4)] cluster and the [Fe(3)S(4)] cluster can be rationalized in terms of the electrostatic influence of the residue 14 side chains, rather than the dissociation constant of this residue, as is the case for [Fe(4)S(4)] ferredoxins. The trends in reduction potentials are in line with there being no electronic coupling between the silver(I) ion and the Fe(3)S(4) fragment.
Asunto(s)
Proteínas Arqueales/química , Complejos de Coordinación/química , Ferredoxinas/química , Hierro/química , Pyrococcus furiosus , Plata/química , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Anisotropía , Proteínas Arqueales/biosíntesis , Proteínas Arqueales/genética , Cristalografía por Rayos X , Espectroscopía de Resonancia por Spin del Electrón , Escherichia coli , Ferredoxinas/biosíntesis , Ferredoxinas/genética , Modelos Moleculares , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Oxidación-Reducción , Ingeniería de Proteínas , Estabilidad ProteicaRESUMEN
Mammalian adrenodoxin (Adx) has been known for many years as an essential electron mediator in mitochondrial cytochrome P450 systems. Because of its ability to support several cytochrome P450 enzymes, it is involved not only in adrenal steroid hormone biosynthesis but also in vitamin D and bile acid metabolism. Recently, Adx is increasingly gaining attention because of its potential for pharmaceutical industry and biotechnology. With human cytochromes P450 becoming important drug targets, suitable Adx-based screening systems have to be developed to test putative new drugs. Moreover, in artificial systems, Adx has been shown to functionally interact with diverse bacterial cytochromes P450 catalyzing a variety of chemically interesting reactions. Putative biotechnological applications of such Adx-containing reconstituted systems are discussed.
Asunto(s)
Adrenodoxina/fisiología , Ferredoxinas/fisiología , Adrenodoxina/biosíntesis , Adrenodoxina/química , Animales , Proteínas Bacterianas/química , Proteínas Bacterianas/fisiología , Coenzimas/biosíntesis , Sistema Enzimático del Citocromo P-450/química , Sistema Enzimático del Citocromo P-450/fisiología , Evaluación Preclínica de Medicamentos , Ferredoxinas/biosíntesis , Ferredoxinas/química , Humanos , Mitocondrias/enzimología , Oxidación-Reducción , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/químicaRESUMEN
Amino-acid selective isotope labeling of proteins offers numerous advantages in mechanistic studies by revealing structural and functional information unattainable from a crystallographic approach. However, efficient labeling of proteins with selected amino acids necessitates auxotrophic hosts, which are often not available. We have constructed a set of auxotrophs in a commonly used Escherichia coli expression strain C43(DE3), a derivative of E. coli BL21(DE3), which can be used for isotopic labeling of individual amino acids or sets of amino acids. These strains have general applicability to either soluble or membrane proteins that can be expressed in E. coli. We present examples in which proteins are selectively labeled with (13)C- and (15)N-amino acids and studied using magic-angle spinning solid-state NMR and pulsed EPR, demonstrating the utility of these strains for biophysical characterization of membrane proteins, radical-generating enzymes and metalloproteins.
Asunto(s)
Complejo IV de Transporte de Electrones/biosíntesis , Proteínas de Escherichia coli/biosíntesis , Ferredoxinas/biosíntesis , Marcaje Isotópico/métodos , Secuencias de Aminoácidos , Sitios de Unión , Espectroscopía de Resonancia por Spin del Electrón , Complejo IV de Transporte de Electrones/química , Escherichia coli/genética , Proteínas de Escherichia coli/química , Ferredoxinas/química , Enlace de Hidrógeno , Hierro/química , Proteínas de la Membrana/biosíntesis , Proteínas de la Membrana/química , Modelos Moleculares , Resonancia Magnética Nuclear Biomolecular , Organismos Modificados Genéticamente , Oxidación-Reducción , Subunidades de Proteína/biosíntesis , Subunidades de Proteína/química , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/química , Espectroscopía Infrarroja por Transformada de Fourier , Azufre/químicaRESUMEN
Photosynthetic organisms experience changes in light quantity and light quality in their natural habitat. In response to changes in light quality, these organisms redistribute excitation energy and adjust photosystem stoichiometry to maximize the utilization of available light energy. However, the response of other cellular processes to changes in light quality is mostly unknown. Here, we report a systematic investigation into the adaptation of cellular processes in Synechocystis species PCC 6803 to light that preferentially excites either photosystem II or photosystem I. We find that preferential excitation of photosystem II and photosystem I induces massive reprogramming of the Synechocystis transcriptome. The rewiring of cellular processes begins as soon as Synechocystis senses the imbalance in the excitation of reaction centers. We find that Synechocystis utilizes the cyclic photosynthetic electron transport chain for ATP generation and a major part of the respiratory pathway to generate reducing equivalents and carbon skeletons during preferential excitation of photosystem I. In contrast, cytochrome c oxidase and photosystem I act as terminal components of the photosynthetic electron transport chain to produce sufficient ATP and limited amounts of NADPH and reduced ferredoxin during preferential excitation of photosystem II. To overcome the shortage of NADPH and reduced ferredoxin, Synechocystis preferentially activates transporters and acquisition pathways to assimilate ammonia, urea, and arginine over nitrate as a nitrogen source. This study provides a systematic analysis of cellular processes in cyanobacteria in response to preferential excitation and shows that the cyanobacterial cell undergoes significant adjustment of cellular processes, many of which were previously unknown.
Asunto(s)
Perfilación de la Expresión Génica , Luz , Complejo de Proteína del Fotosistema I/metabolismo , Complejo de Proteína del Fotosistema II/metabolismo , Synechocystis/metabolismo , Adenosina Trifosfato/biosíntesis , Amoníaco/metabolismo , Arginina/metabolismo , Complejo IV de Transporte de Electrones/metabolismo , Ferredoxinas/biosíntesis , Regulación Bacteriana de la Expresión Génica , Genes Bacterianos , NADP/biosíntesis , Análisis de Secuencia por Matrices de Oligonucleótidos , ARN Bacteriano/metabolismo , Synechocystis/genética , Synechocystis/fisiología , Urea/metabolismoRESUMEN
Ferredoxin (Fd) is the major iron-containing protein in photosynthetic organisms and is central to reductive metabolism in the chloroplast. The Chlamydomonas reinhardtii genome encodes six plant type [Fe2S2] ferredoxins, products of PETF, FDX2-FDX6. We performed the functional analysis of these ferredoxins by localizing Fd, Fdx2, Fdx3, and Fdx6 to the chloroplast by using isoform-specific antibodies and monitoring the pattern of gene expression by iron and copper nutrition, nitrogen source, and hydrogen peroxide stress. In addition, we also measured the midpoint redox potentials of Fd and Fdx2 and determined the kinetic parameters of their reactions with several ferredoxin-interacting proteins, namely nitrite reductase, Fd:NADP+ oxidoreductase, and Fd:thioredoxin reductase. We found that each of the FDX genes is differently regulated in response to changes in nutrient supply. Moreover, we show that Fdx2 (Em = -321 mV), whose expression is regulated by nitrate, is a more efficient electron donor to nitrite reductase relative to Fd. Overall, the results suggest that each ferredoxin isoform has substrate specificity and that the presence of multiple ferredoxin isoforms allows for the allocation of reducing power to specific metabolic pathways in the chloroplast under various growth conditions.
Asunto(s)
Proteínas Algáceas/biosíntesis , Chlamydomonas reinhardtii/metabolismo , Cloroplastos/metabolismo , Ferredoxinas/biosíntesis , Regulación de la Expresión Génica/fisiología , Proteínas Protozoarias/biosíntesis , Proteínas Algáceas/genética , Animales , Secuencia de Bases , Chlamydomonas reinhardtii/genética , Chlamydomonas reinhardtii/crecimiento & desarrollo , Cloroplastos/genética , Cobre/metabolismo , Cobre/farmacología , Ferredoxinas/genética , Regulación de la Expresión Génica/efectos de los fármacos , Genoma del Cloroplasto/fisiología , Peróxido de Hidrógeno/farmacocinética , Hierro/metabolismo , Hierro/farmacología , Datos de Secuencia Molecular , Oxidantes/farmacología , Estrés Oxidativo/efectos de los fármacos , Estrés Oxidativo/fisiología , Oxidorreductasas/genética , Oxidorreductasas/metabolismo , Proteínas Protozoarias/genéticaRESUMEN
The catalytic turnover of cytochrome P450( cam ) from Pseudomonas putida requires two auxiliary reduction partners, putidaredoxin (Pd) and putidaredoxin reductase (PdR). We report the functional expression in Escherichia coli of tricistronic constructs consisting of P450( cam ) encoded by the first cistron and the auxiliary proteins, Pd and PdR by the second and the third. Transformed bacterial whole cells efficiently oxidized (1R)-(+)-camphor to 5-exo-hydroxycamphor and, interestingly, limonene to (-)-perillyl alcohol. These bioengineered E. coli cells possess a heterologous self-sufficient P450 catalytic system that may have advantages in terms of low cost and high yield for the production of fine chemicals.
Asunto(s)
Alcanfor 5-Monooxigenasa/biosíntesis , Escherichia coli/genética , Ferredoxinas/biosíntesis , Dosificación de Gen , NADH NADPH Oxidorreductasas/biosíntesis , Pseudomonas putida/enzimología , Biotransformación , Alcanfor/metabolismo , Alcanfor 5-Monooxigenasa/genética , Ciclohexenos/metabolismo , Escherichia coli/enzimología , Ferredoxinas/genética , Expresión Génica , Limoneno , Monoterpenos/metabolismo , NADH NADPH Oxidorreductasas/genética , Pseudomonas putida/genética , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/genética , Terpenos/metabolismoRESUMEN
Ferredoxin (Fd) is the soluble protein that accepts electrons from photosystem I (PSI) and makes them available to stromal enzymes in higher plant chloroplasts. In linear electron flow, Fd mainly donates electrons to Fd:NADPH reductase (FNR) which generates NADPH for use in the Calvin cycle, but Fd may also return electrons to the thylakoid plastoquinone pool, forming a cyclic electron flow. Many higher plants contain two different photosynthetic Fd proteins, but there are no conserved sequence differences that allow their division into evolutionary groups. In the model C3 photosynthesizing dicot, Arabidopsis thaliana, there are two such photosynthetic Fds, and we have exploited RNA interference (RNAi) techniques to specifically decrease transcript abundance of different Fds in this plant. Surprisingly, the perturbation of photosynthesis, as measured by cholorophyll fluorescence, in RNAi lines of the two different photosynthetic Fds shows opposite trends. Linear electron flow is retarded in lines with lower Fd2 (the most abundant Fd species) levels and under certain circumstances enhanced in lines with lower Fd1 (the minor isoprotein) levels. These data are evidences for at least partially differentiated roles of Fd1 and Fd2 in photosynthetic electron transfer, possibly in the partition of electrons into linear and cyclic electron flow.
Asunto(s)
Arabidopsis/metabolismo , Ferredoxinas/biosíntesis , Fotosíntesis , Interferencia de ARN , Secuencia de Aminoácidos , Arabidopsis/química , Arabidopsis/genética , Arabidopsis/crecimiento & desarrollo , Ferredoxinas/química , Ferredoxinas/genética , Cinética , Datos de Secuencia Molecular , Filogenia , Hojas de la Planta/química , Hojas de la Planta/clasificación , Hojas de la Planta/genética , Hojas de la Planta/metabolismo , Alineación de Secuencia , Homología de Secuencia de AminoácidoRESUMEN
The heterocyst is a specialized cell for nitrogen fixation in some filamentous cyanobacteria. Here we report that a rubrerythrin (RbrA) from Anabaena sp. PCC 7120 functions as a peroxidase in heterocysts and plays an important role in protection of nitrogenase. The electron donor for RbrA in H(2)O(2) reduction is NADPH and the electron transfer from NADPH to RbrA depends on ferredoxin:NADP(+) oxidoreductase. A rbrA mutant (r27) grew much more slowly than the wild type under diazotrophic conditions. Its nitrogenase activity measured in air was only 8% of that measured under anoxic conditions. Staining r27 filaments with 2',7'-dichlorodihydrofluorescein diacetate indicated that heterocysts had a higher H(2)O(2) concentration than the vegetative cells. The expression of rbrA was controlled by two promoters and the promoter for the smaller transcript was regulated by HetR. Spatial expression of rbrA was studied and the results showed that the transcription is localized predominantly in heterocysts. In a mutant lacking nifH and rbrA, the H(2)O(2) concentration in heterocysts was lower than that in the vegetative cells, suggesting that NifH is involved in H(2)O(2) generation. Our results demonstrate that RbrA is a critical enzyme for H(2)O(2) decomposition and provide evidence that nitrogenase autoprotection is important in heterocysts.
Asunto(s)
Anabaena/enzimología , Proteínas Bacterianas/fisiología , Ferredoxinas/fisiología , Peróxido de Hidrógeno/metabolismo , Peróxido de Hidrógeno/toxicidad , Nitrogenasa/efectos de los fármacos , Peroxidasas/fisiología , Aerobiosis , Anabaena/genética , Anabaena/crecimiento & desarrollo , Anaerobiosis , Proteínas Bacterianas/biosíntesis , Proteínas Bacterianas/genética , Ferredoxina-NADP Reductasa/metabolismo , Ferredoxinas/biosíntesis , Ferredoxinas/genética , Regulación Bacteriana de la Expresión Génica , Hemeritrina , NADP/metabolismo , Fijación del Nitrógeno/fisiología , Orgánulos/enzimología , Oxidorreductasas/genética , Oxidorreductasas/fisiología , Peroxidasas/biosíntesis , Peroxidasas/genética , Regiones Promotoras Genéticas , RubredoxinasRESUMEN
Twelve of the fifteen potential P450 enzymes from the bacterium Novosphingobium aromaticivorans, which is known to degrade a wide range of aromatic hydrocarbons, have been produced via heterologous expression in Escherichia coli. The enzymes were tested for their ability to bind a range of substrates including polyaromatic hydrocarbons. While two of the enzymes were found to bind aromatic compounds (CYP108D1 and CYP203A2), the others show binding with a variety of compounds including linear alkanes (CYP153C1) and mono- and sesqui-terpenoid compounds (CYP101B1, CYP101C1, CYP101D1, CYP101D2, CYP111A1, and CYP219A1). A 2Fe-2S ferredoxin (Arx-A), which is associated with CYP101D2, was also produced. The activity of five of the P450 enzymes (CYP101B1, CYP101C1, CYP101D1, CYP101D2, and CYP111A2) was reconstituted with Arx-A and putidaredoxin reductase (of the P450cam system from Pseudomonas putida) in a Class I type electron transfer system. Preliminary characterisation of the majority of the substrate oxidation products is reported.
Asunto(s)
Proteínas Bacterianas , Sistema Enzimático del Citocromo P-450 , Sphingomonadaceae/enzimología , Proteínas Bacterianas/biosíntesis , Proteínas Bacterianas/genética , Proteínas Bacterianas/aislamiento & purificación , Alcanfor/análogos & derivados , Alcanfor/química , Sistema Enzimático del Citocromo P-450/biosíntesis , Sistema Enzimático del Citocromo P-450/genética , Sistema Enzimático del Citocromo P-450/aislamiento & purificación , Ferredoxinas/biosíntesis , Ferredoxinas/genética , Ferredoxinas/aislamiento & purificación , Análisis Espectral , Sphingomonadaceae/genética , Especificidad por SustratoRESUMEN
Thermoanaerobacter tengcongensis, one of many thermophilic organisms, survives harsh living conditions in temperatures ranging from 50 to 80 degrees C. In this comprehensive analysis, we present a robust approach, 2-DE and MALDI-TOF MS, to compare and identify the bacterial proteins responding to the temperature stress. In total, 164 spots of 2-DE were found with the significant changes in spot volume at three culture temperatures, 55, 75, and 80 degrees C, respectively; furthermore, 87 unique proteins were characterized by MS. Our results reveal that the electrophoretic images of the bacterial proteins, extracted from two culture temperatures (55 and 75 degrees C), had similar patterns; however, the bacteria cultured at 80 degrees C had dramatically decreased their spot volumes. Additionally, the temperature-sensitive proteins are broadly divided into two groups: specific expression at certain temperatures and consistent changes of expression responsive to temperature. For instance, three proteins closely related with redox regulation, dihydrolipoamide acyltransferase, NADH:ubiquinone oxidoreductase, and ferredoxin, were only detected in the bacteria cultured at 55 degrees C. Whereas, two chaperonins, GroES and GroEL, were found to show a consistent increase during the elevated temperatures with the determinations, either by MS or Western blot. The proteomic information, thus expedites our understanding of the molecular mechanisms regarding how thermophilic bacteria adapt to the alterations in living environment.
Asunto(s)
Proteínas Bacterianas/metabolismo , Proteoma/química , Thermoanaerobacter/metabolismo , Aciltransferasas/biosíntesis , Western Blotting , Chaperonina 10/biosíntesis , Chaperonina 60/biosíntesis , Complejo I de Transporte de Electrón/biosíntesis , Electroforesis en Gel Bidimensional , Ferredoxinas/biosíntesis , Calor , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Thermoanaerobacter/crecimiento & desarrolloRESUMEN
The human malaria parasite (Plasmodium falciparum) possesses a plastid-derived organelle called the apicoplast, which is believed to employ metabolisms crucial for the parasite's survival. We cloned and studied the biochemical properties of plant-type ferredoxin (Fd) and Fd-NADP+ reductase (FNR), a redox system that potentially supplies reducing power to Fd-dependent metabolic pathways in malaria parasite apicoplasts. The recombinant P. falciparum Fd and FNR proteins were produced by synthetic genes with altered codon usages preferred in Escherichia coli. The redox potential of the Fd was shown to be considerably more positive than those of leaf-type and root-type Fds from plants, which is favourable for a presumed direction of electron flow from catabolically generated NADPH to Fd in the apicoplast. The backbone structure of P. falciparum Fd, as solved by X-ray crystallography, closely resembles those of Fds from plants, and the surface-charge distribution shows several acidic regions in common with plant Fds and some basic regions unique to this Fd. P. falciparum FNR was able to transfer electrons selectively to P. falciparum Fd in a reconstituted system of NADPH-dependent cytochrome c reduction. These results indicate that an NADPH-FNR-Fd cascade is operative in the apicoplast of human malaria parasites.
Asunto(s)
Ferredoxina-NADP Reductasa/química , Ferredoxinas/química , Plasmodium falciparum/enzimología , Animales , Clonación Molecular , Cristalización , Cristalografía por Rayos X , Transporte de Electrón , Ferredoxina-NADP Reductasa/biosíntesis , Ferredoxinas/biosíntesis , Modelos Moleculares , Oxidación-Reducción , Proteínas Recombinantes/aislamiento & purificación , Fracciones Subcelulares/enzimologíaRESUMEN
The model iron-sulfur (Fe-S) protein ferredoxin (Fd) from Synechocystis sp. PCC 6803 has been simultaneously produced and matured in a cell-free production system. After 6 h of incubation at 37 degrees C, Fd accumulated to >450 microg/mL. Essentially all was soluble, and 85% was active. Production and maturation of the protein in the cell-free system were found to be dependent in a coupled manner on the concentration of the supplemented iron and sulfur sources, ferrous ammonium sulfate and cysteine, respectively. The recombinant expression of ISC helper proteins during cell extract preparation did not increase cell-free Fd accumulation or activity, although the efficiency of iron and cysteine utilization increased. Fd maturation was independent of protein production rate, and proceeded at a constant rate throughout the period of active translation. In addition, incubation of denatured apo Fd with cell-free reaction components resulted in recovery of Fd activity, supporting the interpretation that maturation mechanisms did not act co-translationally. Incubation at 28 degrees C increased total and active protein accumulation, but decreased the ratio of active to total Fd produced. In summary, the high product yields and folding efficiency make the cell-free system described here an attractive platform for the study of Fe-S protein production and maturation. The system enables both small-volume, high throughput investigations as well as larger scale production. To our knowledge, this is the first demonstration of directed, high-yield production and maturation of an Fe-S protein in a cell-free system.
Asunto(s)
Ferredoxinas/biosíntesis , Expresión Génica , Proteínas Hierro-Azufre/biosíntesis , Hierro/metabolismo , Azufre/metabolismo , Sistema Libre de Células , Clonación Molecular , Escherichia coli/enzimología , Escherichia coli/genética , Escherichia coli/metabolismo , Ferredoxinas/genética , Genes Bacterianos , Proteínas Hierro-Azufre/genética , Cinética , Proteínas Recombinantes/metabolismoRESUMEN
The plant-type ferredoxin/ferredoxin-NADP(+) reductase (Fd/FNR) redox system found in parasites of the phylum Apicomplexa has been proposed as a target for novel drugs used against life-threatening diseases such as malaria and toxoplasmosis. Like many proteins from these protists, apicomplexan FNRs are characterized by the presence of unique peptide insertions of variable length and yet unknown function. Since three-dimensional data are not available for any of the parasite FNRs, we used limited proteolysis to carry out an extensive study of the conformation of Toxoplasma gondii FNR. This led to identification of 11 peptide bonds susceptible to the action of four different proteases. Cleavage sites are clustered in four regions of the enzyme, which include two of its three species-specific insertions. Such regions are thus predicted to form flexible surface loops. The protein substrate Fd protected FNR against cleavage both at its N-terminal peptide and at its largest sequence insertion (28 residues). Deletion by protein engineering of the species-specific subdomain containing the latter insertion resulted in an enzyme form that, although catalytically active, displayed a 10-fold decreased affinity for Fd. In contrast, removal of the first 15 residues of the enzyme unexpectedly enhanced its interaction with Fd. Thus, two flexible polypeptide regions of T. gondii FNR are involved in Fd interaction but have opposite roles in modulating the binding affinity for the protein ligand. In this respect, T. gondii FNR differs from plant FNRs, where the N-terminal peptide contributes to the stabilization of their complex with Fd.
Asunto(s)
Ferredoxina-NADP Reductasa/metabolismo , Ferredoxinas/metabolismo , Unión Proteica/fisiología , Estructura Terciaria de Proteína/fisiología , Toxoplasma/metabolismo , Secuencia de Aminoácidos , Animales , Ferredoxina-NADP Reductasa/biosíntesis , Ferredoxinas/biosíntesis , Modelos Biológicos , Modelos Moleculares , Datos de Secuencia Molecular , Péptidos/metabolismo , Ingeniería de Proteínas/métodos , Especificidad de la Especie , Relación Estructura-Actividad , Especificidad por SustratoRESUMEN
A thermophilic, obligately chemolithoautotrophic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6, assimilates carbon dioxide via the reductive tricarboxylic acid cycle. A gene encoding a ferredoxin involved in this cycle as an electron donor (HtFd1) was cloned and sequenced. Interestingly, another ferredoxin gene (encoding HtFd2) was found in tandem with the HtFd1 gene. These two ferredoxin genes overlapped by four bp, and transcriptional analysis revealed that they are co-transcribed as an operon. The deduced amino acid sequences of HtFd1 and HtFd2 were 42.9% identical and each contained four cysteine residues that serve as probable ligands to an iron-sulfur cluster. Spectroscopic analyses of the purified recombinant ferredoxins heterologously expressed in Escherichia coli indicated that each ferredoxin contains a single [4Fe-4S]2+/1+ cluster.