Asunto(s)
Enfermedades de los Bovinos/prevención & control , Herpes Simple/veterinaria , Herpesvirus Bovino 2/metabolismo , Timidina Quinasa/análisis , Vacunas Virales/administración & dosificación , Animales , Bovinos , Cobayas , Herpesvirus Bovino 2/inmunología , Herpesvirus Bovino 2/patogenicidad , Vacunas Virales/química , VirulenciaRESUMEN
Polypeptides synthesized in bovine testes cells infected with bovine herpesvirus type 2 were labeled with [35S]methionine and were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Thirty-eight virus-induced proteins, ranging in molecular weight from 32,000 to 149,000, were detectable by analysis of whole cell lysates between postinfection hours 2 and 36. A similar number was immunoprecipitated by rabbit antiserum to bovine herpesvirus type 2. Twelve proteins incorporated [3H]glucosamine. On the basis of their temporal characteristics and their pattern of synthesis in the presence of cycloheximide and dactinomycin, 3 proteins, including at least one that was nondetectable in the absence of drugs, were classified as alpha proteins, 4 as beta proteins, 1 as a beta/gamma protein, and 27 as gamma proteins. Host cell protein synthesis was not reduced substantially until postinfection hours 22 to 28.