RESUMEN
Hymenolepis diminuta a zoonotic tapeworm infection in human remains an important cestode model for anthelmintic study as it display common clinical symptoms like other adult human tapeworms during heavy infestation. The use of Lactobacillus as a probiotic is an alternative to drugs which have increased in research and usage considerably during the last decade. The present study aims to determine the anthelmintic efficacy of two probiotics, L. taiwanensis strain S29 and L. plantarum strain S27 against H. diminuta in infected rat. Four groups of animals, each with six numbers were randomly chosen as the negative control (Group I), positive control (infected) (Group II) and the infected treated with two probiotics Group III and Group IV respectively. Another four groups (Group V-VIII) were selected and further subdivided into four sub-groups to investigate the development of larvae to adult during probiotics treatment. Worm burden, egg per gram were determined after treatment with these two probiotics. Furthermore, hematological assays and levels of biochemical markers were estimated, tissue damage was assayed through histological study and intestinal mitochondria detection was done. Worm sustainability reduced about 70-90% and EPG count decreased by 81-94% in probiotics treated groups. A significant level of unsuccessful establishment of larvae was observed in the developmental phase. Improvement in hematological parameter along with some biochemical parameters in the host were significantly observed after treatment with probiotics. The architecture damaged caused in the intestine and mitochondria density due to parasite infection improved significantly as that of control after probiotics treatment.
Asunto(s)
Antihelmínticos , Infecciones por Cestodos , Himenolepiasis , Lactobacillus plantarum , Probióticos , Humanos , Ratas , Animales , Himenolepiasis/tratamiento farmacológico , Himenolepiasis/parasitología , Suiza , Lactobacillus , Antihelmínticos/uso terapéutico , Infecciones por Cestodos/tratamiento farmacológico , Infecciones por Cestodos/prevención & control , Probióticos/uso terapéuticoRESUMEN
Hymenolepiasis is a helminthic and occasionally fatal disease of human imposing heavy economic losses to human society. Present study was aimed to diagnose the school children for the prevalence and control of Hymenolepiasis. A school based cross-sectional analysis of stool samples collected from 188 children aged 06-15 years was carried out (February to June 2016). Two stool samples were collected from each student before diagnosing and after treatment. The samples were fixed in 10% formalin and observed under the light microscope using the methods of direct smear in Lugol's solution, normal saline and flotation techniques. On the basis of drugs accessibility all the H. nana infected children were divided in to 2- groups. Children in group A were treated with albendazole (bendazol) 400mg once orally, group B was treated with albendazole (zentel) 200mg orally. Eggs per gram of faeces were counted in each group before and after treatment. Of the 188 children, current study reveals only 6.08% (n=18/296) infection with H.nana and 10.5% (n=16/151) were diagnosed with co infections. The % efficacy of albendazole (Zentel) and albendazole (bendazol) against Hymenolepis nana infection was reported as 83% and 75% respectively. Present study was concluded that albendazole (zentel) is the drug of choice for the treatment of hymenolepiasis in children.
Asunto(s)
Albendazol/uso terapéutico , Anticestodos/uso terapéutico , Himenolepiasis/tratamiento farmacológico , Administración Oral , Adolescente , Albendazol/administración & dosificación , Animales , Anticestodos/administración & dosificación , Niño , Estudios Transversales , Heces/parasitología , Femenino , Humanos , Himenolepiasis/epidemiología , Hymenolepis nana/efectos de los fármacos , Óvulo/efectos de los fármacos , Pakistán/epidemiología , Cooperación del Paciente , Resultado del TratamientoRESUMEN
Hymenolepis nana is a common intestinal tapeworm that affects humans. Drugs are available for the treatment of this infection, including praziquantel (PZQ), nitazoxanide and niclosamide. Although the drug of choice is praziquantel, due to its high cure rates, indicators of the development of PZQ resistance by different parasites have begun to appear over recent decades. Therefore, this study was a trial to find an alternative to PZQ by assessing the activity of the crude aqueous extract of the medicinal herb Artemisia absinthium against H. nana. In vitro, the extract was used against adult worms at concentrations of 1 and 5 mg/ml, in comparison with 1 mg/ml of PZQ. The times of worm paralysis and death were determined. Ultrastructural morphological changes were studied using transmission electron microscopy (TEM). For the in vivo study, infected mice were divided into untreated, PZQ-treated and A. absinthium-treated groups (400 mg/kg and 800 mg/kg). Pre- and post-treatment egg counts per gram of faeces (EPG) were performed; then, the reduction percentages of the EPG and worm burden were calculated. The best results were obtained with praziquantel. Artemisia absinthium induced worm paralysis, death and ultrastructural alterations, such as tegumental damage, lipid accumulation, and destruction of the nephridial canal and the intrauterine eggs, in a dose-dependent manner. Additionally, significant reductions in the EPG and worm burden were recorded in A. absinthium-treated mice. Although the results obtained with A. absinthium were promising and comparable to PZQ, further studies using different extracts, active ingredients and concentrations against different parasites should be conducted.
Asunto(s)
Artemisia absinthium/química , Hymenolepis nana/efectos de los fármacos , Extractos Vegetales/farmacología , Extractos Vegetales/uso terapéutico , Praziquantel/farmacología , Praziquantel/uso terapéutico , Animales , Antihelmínticos/administración & dosificación , Antihelmínticos/farmacología , Antihelmínticos/uso terapéutico , Heces/parasitología , Himenolepiasis/tratamiento farmacológico , Himenolepiasis/parasitología , Hymenolepis nana/ultraestructura , Ratones , Microscopía Electrónica de Transmisión , Recuento de Huevos de Parásitos , Extractos Vegetales/administración & dosificación , Praziquantel/administración & dosificaciónRESUMEN
The potential therapeutic value of Moringa oleifera extract (MOE), due to its anti-inflammatory and anti-oxidant effects, has been reported previously. In this study, Hymenolepis nana antigen (HNA) in combination with MOE was used in immunization against H. nana infection. Adult worm and egg counts were taken, while histological changes in the intestine were observed. Mucosal mast (MMCs) and goblet cells (GCs) were stained with specific stains, while serum and intestinal IgA were assayed using enzyme-linked immunosorbent assay (ELISA). Reduced glutathione (GSH) and lipid peroxidation (thiobarbituric acid reactive substances, TBARS) were assayed. Real-time polymerase chain reaction (PCR) was used for detection of mRNA expression in ileum tissue. The results demonstrated an improvement in the architecture of intestinal villi, decreased inducible nitric oxide synthase (iNOs) and TBARS, and increased GSH in HNA, MOE and MOE + HNA groups. In the same groups, an increase in GCs, mucin 2 (MUC2), interleukins (IL)-4, -5 and -9, and stem cell factor (SCF) versus a decrease in both interferon-gamma (IFN-γ) and transforming growth factor (TGF-ß) expression appeared. HNA and MOE + HNA increased serum and intestinal IgA, respectively. MOE decreased MMCs and achieved the highest reductions in both adult worms and eggs. In conclusion, MOE could achieve protection against H. nana infections through decreased TGF-ß, IFN-γ and MMC counts versus increased GC counts, T-helper cell type 2 (Th2) cytokines and IgA level.
Asunto(s)
Himenolepiasis/tratamiento farmacológico , Hymenolepis/efectos de los fármacos , Intestinos/efectos de los fármacos , Intestinos/inmunología , Moringa oleifera/química , Extractos Vegetales/uso terapéutico , Animales , Antihelmínticos/administración & dosificación , Antihelmínticos/química , Antihelmínticos/uso terapéutico , Citocinas/efectos de los fármacos , Citocinas/inmunología , Glutatión/análisis , Himenolepiasis/inmunología , Himenolepiasis/parasitología , Inmunoglobulina A/análisis , Inmunoglobulina A/inmunología , Interferón gamma/efectos de los fármacos , Interferón gamma/genética , Interferón gamma/inmunología , Intestinos/parasitología , Peroxidación de Lípido , Ratones , Óxido Nítrico Sintasa/efectos de los fármacos , Óxido Nítrico Sintasa/genética , Recuento de Huevos de Parásitos , Extractos Vegetales/administración & dosificación , Células Th2/efectos de los fármacos , Células Th2/inmunologíaRESUMEN
After cannibalism had appeared in the reproductive units of a white mouse colony, treatment against confirmed Hymenolepis nana, a tapeworm with zoonotic potential, was performed on 67 mice in the reproductive and nursery units. Faecal droppings were evaluated by flotation and sedimentation methods. The sedimentation method revealed a higher number of positive results before, during and after the treatment, but the flotation method yielded some additional positive cases. In the reproductive unit, H. nana eggs were confirmed in 50% of the tested mice by the flotation and in 70% by the sedimentation method. In the nursery units, H. nana eggs were detected in 10.5% of the tested mice by the flotation and in 24.6% by the sedimentation method. A colony of mice was treated against the tapeworm H. nana with praziquantel and emodepside in doses of 2.574 mg praziquantel/100 g body mass and of 0.642 mg emodepside/100 g body mass. The content of the original pipettes (Profender®) was applied as a spot-on on the back of the neck in the area between the shoulders. The application was repeated three times at 14-day intervals. Seven days after the third therapy no H. nana was found in any of the tested mice in the reproductive or the nursery units. After the treatment, cannibalism was no longer observed. This treatment represented one of the steps aimed at improving animal welfare and preventing potential zoonotic disease. The public health significance of this cestode should receive more attention, especially among people who take care of mice, have them as pets, or feed them to reptiles.
Asunto(s)
Himenolepiasis/veterinaria , Hymenolepis nana , Enfermedades de los Roedores/parasitología , Bienestar del Animal , Animales , Antihelmínticos/uso terapéutico , Femenino , Himenolepiasis/tratamiento farmacológico , Himenolepiasis/parasitología , Ciencia de los Animales de Laboratorio , Masculino , Ratones , Praziquantel/uso terapéutico , Enfermedades de los Roedores/tratamiento farmacológicoRESUMEN
AbstractWe describe the case of a 43-year-old human immunodeficiency virus-infected man receiving combined antiretroviral therapy and coinfected with Hymenolepis nana, Hymenolepis diminuta, and Giardia intestinalis, presenting as chronic diarrhea and critical weight loss. Immunological aspects of these interactions are reviewed.
Asunto(s)
Diarrea/diagnóstico , Giardiasis/diagnóstico , Infecciones por VIH/diagnóstico , Himenolepiasis/diagnóstico , Adulto , Animales , Antihelmínticos/uso terapéutico , Fármacos Anti-VIH/uso terapéutico , Antiprotozoarios/uso terapéutico , Coinfección , Diarrea/tratamiento farmacológico , Diarrea/parasitología , Diarrea/virología , Giardia lamblia/efectos de los fármacos , Giardia lamblia/crecimiento & desarrollo , Giardiasis/tratamiento farmacológico , Giardiasis/parasitología , VIH/efectos de los fármacos , VIH/crecimiento & desarrollo , Infecciones por VIH/tratamiento farmacológico , Infecciones por VIH/virología , Humanos , Himenolepiasis/tratamiento farmacológico , Himenolepiasis/parasitología , Hymenolepis diminuta/efectos de los fármacos , Hymenolepis diminuta/crecimiento & desarrollo , Hymenolepis nana/efectos de los fármacos , Hymenolepis nana/crecimiento & desarrollo , Masculino , Metronidazol/análogos & derivados , Metronidazol/uso terapéutico , Nitrocompuestos , Praziquantel/uso terapéutico , Tiazoles/uso terapéutico , Resultado del Tratamiento , Pérdida de PesoAsunto(s)
Antihelmínticos/administración & dosificación , Hidrocarburos Clorados/administración & dosificación , Himenolepiasis/tratamiento farmacológico , Hymenolepis nana/efectos de los fármacos , Animales , Antihelmínticos/síntesis química , Antihelmínticos/química , Modelos Animales de Enfermedad , Humanos , Hidrocarburos Clorados/síntesis química , Hidrocarburos Clorados/química , Himenolepiasis/parasitología , Hymenolepis nana/patogenicidad , RatonesRESUMEN
Bolivia is one of the countries with a high intestinal helminth and protozoan infection rate. Despite the high prevalence of the parasitic infection, nationwide preventive measures for Bolivian children have not yet been implemented. We evaluated the effect of mass stool examination and treatment as a strategy for decreasing the infection rate. This study was conducted between 2013 and 2015 in children aged 2-18 years. A total of 2,033 stool samples (575 in 2013, 815 in 2014 and 642 in 2015) were collected and examined using the formalin-ether medical sedimentation method. As an anthelminthic medicine, nitazoxanide was given to all infected children within 2 months post-examination, each year. The effect of mass stool examination and treatment was evaluated based on the changes in the overall or individual parasitic infection rates during the study period. The overall parasitic infection rate decreased significantly from 65.2% in 2013 to 43.0% in 2015; a 22.2 percentage point decrease (P<0.001). Protozoan infection accounted for a large portion of the parasitic infections, in the following rates: 62.4% in 2013, 49.3% in 2014, and 41.0% in 2015. The rate of the most common helminth infection, Hymenolepis nana, decreased significantly from 9.0% in 2013 to 6.4% in 2014 to 3.4% in 2015 (P<0.001). Prevalence of the most common pathogenic protozoan infection, Entamoeba histolytica, decreased significantly from 19.0% in 2013 to 3.0% in 2015 (P<0.001). Conversely, the rate of Giardia intestinalis increased significantly from 16.5% in 2013 to 21.2% in 2015 (P<0.01). Mass stool examination and treatment for intestinal helminth and protozoan infections was effective for decreasing the overall parasitic infection rate in the study population, excluding Giardia intestinalis. Further studies on the long-term effect of mass stool examination and treatment for decreasing all intestinal parasitic infection rates in Bolivian children are needed.
Asunto(s)
Entamebiasis/tratamiento farmacológico , Heces/parasitología , Giardiasis/tratamiento farmacológico , Helmintiasis/tratamiento farmacológico , Himenolepiasis/tratamiento farmacológico , Parasitosis Intestinales/tratamiento farmacológico , Parasitosis Intestinales/epidemiología , Tamizaje Masivo , Infecciones por Protozoos/epidemiología , Adolescente , Animales , Bolivia/epidemiología , Niño , Preescolar , Estudios Transversales , Entamoeba histolytica/aislamiento & purificación , Entamebiasis/epidemiología , Entamebiasis/parasitología , Entamebiasis/prevención & control , Femenino , Giardia lamblia/aislamiento & purificación , Giardiasis/epidemiología , Giardiasis/parasitología , Giardiasis/prevención & control , Helmintiasis/epidemiología , Helmintiasis/parasitología , Helmintiasis/prevención & control , Humanos , Himenolepiasis/epidemiología , Himenolepiasis/parasitología , Himenolepiasis/prevención & control , Hymenolepis/aislamiento & purificación , Parasitosis Intestinales/prevención & control , Masculino , Nitrocompuestos , Prevalencia , Estudiantes , Tiazoles/uso terapéutico , Adulto JovenRESUMEN
It was established that steroidal genins and their glycosides of the spirostan series and (especially) furostan series show anticestodal activity against Hymeiolepis nana species. Search for anthelminthic agents in the indicated series of compounds is a promising direction of research.
Asunto(s)
Anticestodos/farmacología , Glicósidos/farmacología , Himenolepiasis/tratamiento farmacológico , Hymenolepis nana/efectos de los fármacos , Espirostanos/farmacología , Esteroles/farmacología , Allium/química , Animales , Anticestodos/aislamiento & purificación , Glicósidos/aislamiento & purificación , Himenolepiasis/parasitología , Hymenolepis nana/fisiología , Solanum lycopersicum/química , Masculino , Ratones , Recuento de Huevos de Parásitos , Pruebas de Sensibilidad Parasitaria , Praziquantel/farmacología , Espirostanos/aislamiento & purificación , Esteroles/aislamiento & purificaciónAsunto(s)
Antihelmínticos/síntesis química , Carbamatos/síntesis química , Himenolepiasis/tratamiento farmacológico , Hymenolepis nana/efectos de los fármacos , Salicilanilidas/síntesis química , Animales , Antihelmínticos/aislamiento & purificación , Antihelmínticos/farmacología , Carbamatos/aislamiento & purificación , Carbamatos/farmacología , Himenolepiasis/parasitología , Himenolepiasis/patología , Hymenolepis nana/crecimiento & desarrollo , Ratones , Recuento de Huevos de Parásitos , Salicilanilidas/aislamiento & purificación , Salicilanilidas/farmacología , Ácido Salicílico/química , Cigoto/efectos de los fármacos , Cigoto/fisiologíaRESUMEN
Context Plants and plant products have been used in traditional medicine as anthelmintic agents in human and veterinary medicine. Three species of Senna plant, S. alata (L), S. alexandrina (M) and S. occidentalis (L.) Link (Fabaceae) have been shown to have a vermicidal/vermifugal effect on a zoonotic tapeworm Hymenolepis diminuta (Rudolphi) (Cyclophyllidean). Objective The present study validates the mode of action of these Senna plants on the parasite. The alcoholic leaf extract was determined to obtain information on the intracellular free calcium concentration level. Materials and methods Hymenolepis diminuta was maintained in Sprague-Dawley rat model for 2 months. Live parasites collected from infected rat intestine were exposed to 40 mg/mL concentration of each plant extracts prepared in phosphate buffer saline at 37 °C, till parasite gets paralyzed. The rate of efflux of calcium from the parasite tissue to the medium and the level of intracellular Ca(2+ )concentration were determined by an atomic absorption spectroscopy. Results This study revealed that exposure of the worms to the plant extract leads to disruption in intracellular calcium homeostasis. A significant increase (44.6% and 25%) of efflux in Ca(2+ )from the tissue to the incubated medium was observed. Senna alata showed high rate of efflux (5.32 mg/g) followed by S. alexandria and S. occidentalis (both 4.6 mg/g) compared with control (3.68 mg/g). Discussion and conclusion These results suggest that leaf extracts caused membrane permeability to Ca(2+ )after vacuolization of the tegument under stress and the extracts may contain compound that can be used as a chemotherapeutic agent.
Asunto(s)
Anticestodos/farmacología , Calcio/metabolismo , Himenolepiasis/tratamiento farmacológico , Hymenolepis diminuta/efectos de los fármacos , Intestinos/microbiología , Extracto de Senna/farmacología , Senna , Animales , Anticestodos/aislamiento & purificación , Modelos Animales de Enfermedad , Homeostasis , Himenolepiasis/parasitología , Himenolepiasis/transmisión , Hymenolepis diminuta/crecimiento & desarrollo , Hymenolepis diminuta/metabolismo , Fitoterapia , Plantas Medicinales , Ratas Sprague-Dawley , Extracto de Senna/aislamiento & purificación , Senna/química , Factores de TiempoRESUMEN
Hymenolepis diminuta is a natural parasite of the common brown rat Rattus norvegicus, and provides a convenient model system for the assessment of the anthelmintic activity of novel drugs against cestodes. The experiments described in this paper indicate that treatment of rats infected with H. diminuta with a supernatant extract of papaya latex, containing a mixture of four cysteine proteinases, was moderately efficacious, resulting in a significant, but relatively small, reduction in worm burden and biomass. However, faecal egg output was not affected by treatment. In our experiments these effects were only partially dose-dependent, although specific inhibition by E-64 confirmed the role of cysteine proteinases as the active principles in papaya latex affecting worm growth but not statistically reducing worm burden. Data collected for a further 7 days after treatment indicated that the effects of papaya latex supernatant on worm loss and on worm growth were not enhanced. Our findings provide a starting point for further refinement in formulation and delivery, or assessment of alternative natural plant-derived cysteine proteinases in efforts to develop these naturally occurring enzymes into broad-spectrum anthelmintics, with efficacy against cestodes as well as nematodes.
Asunto(s)
Antihelmínticos/farmacología , Proteasas de Cisteína/farmacología , Himenolepiasis/veterinaria , Hymenolepis diminuta/efectos de los fármacos , Extractos Vegetales/farmacología , Enfermedades de los Roedores/tratamiento farmacológico , Animales , Antihelmínticos/administración & dosificación , Antihelmínticos/aislamiento & purificación , Carica/química , Proteasas de Cisteína/administración & dosificación , Proteasas de Cisteína/aislamiento & purificación , Himenolepiasis/tratamiento farmacológico , Masculino , Recuento de Huevos de Parásitos , Carga de Parásitos , Extractos Vegetales/administración & dosificación , Extractos Vegetales/aislamiento & purificación , Proteínas de Plantas/administración & dosificación , Proteínas de Plantas/aislamiento & purificación , Proteínas de Plantas/farmacología , Ratas , Resultado del TratamientoRESUMEN
Worldwide, traditional usage of herbal medicines is a common practice to treat various parasitic infections. In India, bark decoction of Oroxylum indicum (L.) Kurz. (Bignoniaceae) is used as a traditional medicine to cure intestinal-helminthic infections. This study investigated the anthelmintic efficacy of methanolic bark extract of O. indicum on Hymenolepis diminuta (Cestoda), using both in vitro and in vivo methods. Utilizing a mini-questionnaire, first, we collected information about the pattern of anthelmintic use of this plant. Later, in vitro efficacy of extract was tested at 10, 20 and 30 mg/ml on both the artificially excysted juveniles and adult H. diminuta worms. Herein, scanning electron microscopy (SEM) was also utilized to determine the possible effects of extract on tegumental surfaces of juvenile and adult cestode. In vivo, extract was tested at 250, 500 and 1000 mg/kg in H. diminuta albino rat model, against juvenile and adult cestode. Praziquantel (PZQ) served as reference drug in anthelmintic assays. The acute toxicity of extract was determined as per the OECD guidelines. The field questionnaire data revealed that 78 % of people in the area use O. indicum stem bark against intestinal helminths, and of these, 75 % of people also believed it highly efficacious anthelmintic remedy. In vitro testing of extract revealed significant effects on juvenile worms, and 30 mg/ml of extract caused mortality of juveniles at the initial period (0.25 ± 0.00 h). Conversely, PZQ (1 mg/ml) showed paralysis and mortality of juvenile cestodes in 0.44 ± 0.04 and 1.11 ± 0.06 h, respectively. As determined by SEM, in vitro exposure to extract showed substantial effects on both juveniles and adult worms in the form of wrinkled scolex, distorted tegument and eroded microtriches. In vivo study revealed better efficacy of extract against juveniles than adult stages of parasite. Treatment of rats with 1000 mg/kg of extract caused 79.3 % reduction in EPG counts and 70.8 % of reduction in worm counts, which compared well to PZQ efficacy. In acute toxicity study, administration of a single oral dose of 2000 mg/kg of extract to mice did not cause any signs of toxicity or mortality within 2-week observation. Hence, the LD50 of extract was estimated to be greater than 2000 mg/kg. In conclusion, the bark extract of O. indicum possesses concentration-dependent effects against the larval and adult H. diminuta worms, and thus the extract holds promise in the control of intestinal helminthiases. This also supports the common belief of people about the reputation of this plant as anthelmintic in traditional medicine. There is a need to standardize the formulations of this plant for their possible use as standardized herbal anthelmintic agents in the future.
Asunto(s)
Antihelmínticos/farmacología , Bignoniaceae/química , Hymenolepis diminuta/efectos de los fármacos , Extractos Vegetales/farmacología , Animales , Antihelmínticos/toxicidad , Femenino , Himenolepiasis/tratamiento farmacológico , Himenolepiasis/parasitología , Hymenolepis diminuta/ultraestructura , India , Parasitosis Intestinales/tratamiento farmacológico , Parasitosis Intestinales/parasitología , Dosificación Letal Mediana , Masculino , Medicina Tradicional , Ratones , Microscopía Electrónica de Rastreo , Corteza de la Planta/química , Extractos Vegetales/toxicidad , Praziquantel/farmacología , Distribución Aleatoria , Ratas , Ratas Wistar , Encuestas y CuestionariosAsunto(s)
Antihelmínticos , Hidrocarburos Bromados , Hidrocarburos Clorados , Himenolepiasis/tratamiento farmacológico , Hymenolepis nana , Salicilanilidas , Animales , Antihelmínticos/síntesis química , Antihelmínticos/química , Antihelmínticos/farmacología , Modelos Animales de Enfermedad , Evaluación Preclínica de Medicamentos , Hidrocarburos Bromados/síntesis química , Hidrocarburos Bromados/química , Hidrocarburos Bromados/farmacología , Hidrocarburos Clorados/síntesis química , Hidrocarburos Clorados/química , Hidrocarburos Clorados/farmacología , Ratones , Salicilanilidas/síntesis química , Salicilanilidas/química , Salicilanilidas/farmacologíaAsunto(s)
Antihelmínticos , Hidrocarburos Bromados , Himenolepiasis/tratamiento farmacológico , Hymenolepis nana , Salicilanilidas , Animales , Antihelmínticos/síntesis química , Antihelmínticos/química , Antihelmínticos/farmacología , Modelos Animales de Enfermedad , Hidrocarburos Bromados/síntesis química , Hidrocarburos Bromados/química , Hidrocarburos Bromados/farmacología , Ratones , Salicilanilidas/síntesis química , Salicilanilidas/química , Salicilanilidas/farmacologíaRESUMEN
Unlike Hymenolepis nana that transmits directly from person to person, the transmission of Hymenolepis diminuta to human is via accidentally ingesting of arthropods carrying cysticercoid larvae as intermediate host. In places with poor hygienic conditions, this cestod may cause seldom infections especially in children. Studies carried out on various populations have reported the prevalence rate of H.diminuta between 0.001% and 5.5%. Although the reported cases are mostly children, the disease can be seen in every age group. In this report, a pediatric case of H.diminuta infection is presented. A twenty one-month-old male patient with the symptoms of vomiting 3-4 times a day along with mud-like diarrhea continuing for a week was admitted to the pediatric outpatient clinic. According to the history, it was learned that the house where he lived was above a barn and there was a history of insect swallowing. Laboratory findings revealed iron-deficiency anemia. The macroscopic appearance of the stool was in a pale clay-like form, and by direct microscopic examination with lugol solution, 70-75 µm in diameter, thick-shelled and six central hookleted eggs that are characteristic for H.diminuta were identified. A six-day course of oral niclosamide was administered to the patient beginning with 500 mg on the first day and 250 mg on the following five days, together with the treatment for the iron deficiency anemia. After fifteen days, the oral niclosamide treatment was repeated. No H.diminuta eggs were detected in the parasitological examination performed one month after completion of the second round of treatment. This case has been presented to call attention to the importance of patient anamnesis and microscopic examination in the diagnosis of H.diminuta infection which is a rarely seen parasitosis.
Asunto(s)
Anticestodos/administración & dosificación , Himenolepiasis/diagnóstico , Hymenolepis diminuta/aislamiento & purificación , Niclosamida/administración & dosificación , Administración Oral , Anemia Ferropénica/etiología , Anemia Ferropénica/terapia , Animales , Diarrea , Ingestión de Alimentos , Heces/parasitología , Humanos , Himenolepiasis/tratamiento farmacológico , Himenolepiasis/parasitología , Himenolepiasis/transmisión , Hymenolepis diminuta/clasificación , Lactante , Insectos Vectores/parasitología , Masculino , Enfermedades Raras/diagnóstico , Enfermedades Raras/parasitología , VómitosRESUMEN
Anthelmintic resistance against most of the commercial drugs is a great threat to humans as well as the veterinary live stocks. Hence, new treatment strategies to control helminth infections are essential at this hour. Carex baccans Nees has been traditionally used by Jaintia tribes in Northeast India to get rid of intestinal worm infections. Therefore, the present study was conducted to evaluate in vivo cestocidal activity of root tuber extract of C. baccans and its active component resveratrol against the zoonotic cestode Hymenolepis diminuta in the experimental model rat. The cestocidal activity was determined by monitoring the eggs per gram (EPG) counts in faeces of different treated groups. The result showed that the highest dose of the plant extract (50 mg/kg) and resveratrol (4.564 mg/kg body weight) has significant anthelmintic efficacy against H. diminuta. Crude extract of the plant as well as resveratrol reduced EPG count (56.012 and 46.049 %) and also resulted in decreased worm burden by 44.287 and 31.034 %, respectively. The efficacy of the crude extract and resveratrol can be compared to the reference drug praziquantel. The results exhibits considerable cestocidal potential of root tuber crude extract of C. baccans and resveratrol and justify its folklore use.