RESUMEN
Small ruminant lentiviruses (SRLVs) are widespread and infect goats and sheep. Several reports also suggest that SRLVs can infect wild ruminants. The presence of specific antibodies against SRLVs has been identified in wild ruminants from Poland, but no studies have been conducted to detect proviral DNA of SRLVs in these animals. Therefore, the purpose of this study was to examine samples from Polish wild ruminants to determine whether these animals can serve as reservoirs of SRLVs under natural conditions. A total of 314 samples were tested from red deer (n = 255), roe deer (n = 52) and fallow deer (n = 7) using nested real-time PCR. DNA from positive real-time PCR samples was subsequently used to amplify a CA fragment (625 bp) of the gag gene, a 1.2 kb fragment of the pol gene and an LTR-gag fragment. Three samples (0.95%) were positive according to nested real-time PCR using primers and probe specific for CAEV (SRLV group B). All the samples were negative for the primers and probe specific for MVV (SRLV A group). Only SRLV LTR-gag sequences were obtained from two red deer. Phylogenetic analysis revealed that these sequences were more closely related to CAEV than to MVV. Our results revealed that deer can carry SRLV proviral sequences and therefore may play a role in the epidemiology of SRLVs. To our knowledge, this is the first study describing SRLV sequences from red deer.
Asunto(s)
ADN Viral , Ciervos , Infecciones por Lentivirus , Provirus , Animales , Ciervos/virología , Polonia/epidemiología , Provirus/genética , Infecciones por Lentivirus/veterinaria , Infecciones por Lentivirus/virología , Infecciones por Lentivirus/epidemiología , ADN Viral/genética , Lentivirus/aislamiento & purificación , Lentivirus/genética , Lentivirus/clasificación , Filogenia , Reacción en Cadena en Tiempo Real de la Polimerasa/veterinariaRESUMEN
The high genetic heterogeneity of small ruminant lentiviruses (SRLV) renders the genetic characterization of the circulating strains crucial for the epidemiological investigation and the designation of effective diagnostic tools. In Greece, research data regarding the genetic diversity of the circulating SRLV strains is scarce, hindering the implementation of efficient surveillance and control programs. The objective of the study was to genetically characterize SRLV strains isolated from intensive dairy sheep farms in Greece and evaluate the variability of the immunodominant regions of the capsid protein. For this reason, a total of 12 SRLV-infected animals from four intensive dairy sheep farms with purebred Chios and Lacaune ewes were used for the amplification and sequencing of an 800 bp gag-pol fragment. The phylogenetic analyses revealed a breed-related circulation of strains; Chios ewes were infected with strains belonging exclusively to a separate group of genotype A, whereas strains belonging to subtype B2 were isolated from Lacaune ewes. Immunodominant epitopes of capsid protein were quite conserved among the strains of the same genotype, except for the Major Homology Region which showed some unique mutations with potential effects on viral evolution. The present study contributes to the extension of the current knowledge regarding the genetic diversity of SRLV strains circulating in sheep in Greece. However, broader genetic characterization studies are warranted for the exploration of possible recombinant events and the more comprehensive classification of the circulating strains.
Asunto(s)
Variación Genética , Genotipo , Infecciones por Lentivirus , Filogenia , Enfermedades de las Ovejas , Animales , Ovinos , Grecia , Enfermedades de las Ovejas/virología , Infecciones por Lentivirus/veterinaria , Infecciones por Lentivirus/virología , Femenino , Proteínas de la Cápside/genética , Lentivirus/genética , Lentivirus/aislamiento & purificación , Lentivirus/clasificaciónRESUMEN
Small ruminant lentiviruses (SRLV) are viruses that retro-transcribe RNA to DNA and show high rates of genetic variability. SRLV affect animals with strains specific for each host species (sheep or goats), resulting in a series of clinical manifestations depending on the virulence of the strain, the host's genetic background and farm production system. The aim of this work was to present an up-to-date overview of the genomic epidemiology and genetic diversity of SRLV in Italy over time (1998-2019). In this study, we investigated 219 SRLV samples collected from 17 different Italian regions in 178 geographically distinct herds by CEREL. Our genetic study was based on partial sequencing of the gag-pol gene (800 bp) and phylogenetic analysis. We identified new subtypes with high heterogeneity, new clusters and recombinant forms. The genetic diversity of Italian SRLV strains may have diagnostic and immunological implications that affect the performance of diagnostic tools. Therefore, it is extremely important to increase the control of genomic variants to improve the control measures.
Asunto(s)
Infecciones por Lentivirus , Lentivirus/clasificación , Rumiantes/virología , Animales , Enfermedades de las Cabras/virología , Cabras , Italia/epidemiología , Infecciones por Lentivirus/epidemiología , Infecciones por Lentivirus/veterinaria , Infecciones por Lentivirus/virología , Ovinos , Enfermedades de las Ovejas/virologíaRESUMEN
Small ruminant lentiviruses (SRLVs) are a group of highly divergent viruses responsible for global infection in sheep and goats. In a previous study we showed that SRLV strains found in mixed flocks in Poland belonged to subtype A13 and A18, but this study was restricted only to the few flocks from Malopolska region. The present work aimed at extending earlier findings with the analysis of SRLVs in mixed flocks including larger numbers of animals and flocks from different part of Poland. On the basis of gag and env sequences, Polish SRLVs were assigned to the subtypes B2, A5, A12, and A17. Furthermore, the existence of a new subtypes, tentatively designed as A23 and A24, were described for the first time. Subtypes A5 and A17 were only found in goats, subtype A24 has been detected only in sheep while subtypes A12, A23, and B2 have been found in both sheep and goats. Co-infection with strains belonging to different subtypes was evidenced in three sheep and two goats originating from two flocks. Furthermore, three putative recombination events were identified within gag and env SRLVs sequences derived from three sheep. Amino acid (aa) sequences of immunodominant epitopes in CA protein were well conserved while Major Homology Region (MHR) had more alteration showing unique mutations in sequences of subtypes A5 and A17. In contrast, aa sequences of surface glycoprotein exhibited higher variability confirming type-specific variation in the SU5 epitope. The number of potential N-linked glycosylation sites (PNGS) ranged from 3 to 6 in respective sequences and were located in different positions. The analysis of LTR sequences revealed that sequences corresponding to the TATA box, AP-4, AML-vis, and polyadenylation signal (poly A) were quite conserved, while considerable alteration was observed in AP-1 sites. Interestingly, our results revealed that all sequences belonging to subtype A17 had unique substitution T to A in the fifth position of TATA box and did not have a 11 nt deletion in the R region which was noted in other sequences from Poland. These data revealed a complex picture of SRLVs population with ovine and caprine strains belonging to group A and B. We present strong and multiple evidence of dually infected sheep and goats in mixed flocks and present evidence that these viruses can recombine in vivo.
Asunto(s)
Cabras/virología , Infecciones por Lentivirus/transmisión , Lentivirus/genética , Recombinación Genética , Ovinos/virología , Animales , Lentivirus/clasificación , Infecciones por Lentivirus/virología , Filogenia , Secuencias Repetidas TerminalesRESUMEN
Small ruminant lentiviruses (SRLVs) infections lead to chronic diseases and remarkable economic losses undermining health and welfare of animals and the sustainability of farms. Early and definite diagnosis of SRLVs infections is the cornerstone for any control and eradication efforts; however, a "gold standard" test and/or diagnostic protocols with extensive applicability have yet to be developed. The main challenges preventing the development of a universally accepted diagnostic tool with sufficient sensitivity, specificity, and accuracy to be integrated in SRLVs control programs are the genetic variability of SRLVs associated with mutations, recombination, and cross-species transmission and the peculiarities of small ruminants' humoral immune response regarding late seroconversion, as well as intermittent and epitope-specific antibody production. The objectives of this review paper were to summarize the available serological and molecular assays for the diagnosis of SRLVs, to highlight their diagnostic performance emphasizing on advantages and drawbacks of their application, and to discuss current and future perspectives, challenges, limitations and impacts regarding the development of reliable and efficient tools for the diagnosis of SRLVs infections.
Asunto(s)
Infecciones por Lentivirus/diagnóstico , Infecciones por Lentivirus/inmunología , Lentivirus/genética , Lentivirus/inmunología , Rumiantes/virología , Pruebas Serológicas/veterinaria , Animales , Virus de la Artritis-Encefalitis Caprina/genética , Virus de la Artritis-Encefalitis Caprina/inmunología , Enfermedades de las Cabras/diagnóstico , Enfermedades de las Cabras/virología , Cabras/virología , Lentivirus/clasificación , Lentivirus/aislamiento & purificación , Seroconversión , Pruebas Serológicas/métodos , Ovinos/virología , Enfermedades de las Ovejas/diagnóstico , Enfermedades de las Ovejas/virología , Virología/métodos , Virus Visna-Maedi/genética , Virus Visna-Maedi/inmunologíaRESUMEN
Maedi-visna virus (MVV) and caprine arthritis encephalitis virus (CAEV), referred to as small ruminant lentiviruses (SRLVs), belong to the genus Lentivirus of the Retroviridae family. SRLVs infect both sheep and goats, causing significant economic losses and animal welfare damage. Recent findings suggest an association between serological status and allelic variants of different genes such as TMEM154, TLR9, MYD88 and CCR5. The aim of this work was to investigate the role of specific polymorphisms of these genes in SRLVs infection in some sheep flocks in Italy. In addition to those already known, novel variants in the TMEM154 (P7H, I74V, I105V) gene were detected in this study. The risk of infection was determined finding an association between the serological status and polymorphisms P7H, E35K, N70I, I74V, I105V of TMEM154, R447Q, A462S and G520R in TLR9 gene, H176H* and K190K* in MYD88 genes, while no statistical association was observed for the 4-bp deletion of the CCR5 gene. Since no vaccines or treatments have been developed, a genetically based approach could be an innovative strategy to prevent and to control SRLVs infection. Our findings are an important starting point in order to define the genetic resistance profile towards SRLVs infection.
Asunto(s)
Resistencia a la Enfermedad/genética , Infecciones por Lentivirus/genética , Infecciones por Lentivirus/veterinaria , Lentivirus/genética , Proteínas de la Membrana/genética , Factor 88 de Diferenciación Mieloide/genética , Polimorfismo de Nucleótido Simple , Receptores CCR5/genética , Receptor Toll-Like 9/genética , Animales , Variación Genética , Italia , Lentivirus/clasificación , Infecciones por Lentivirus/inmunología , Infecciones por Lentivirus/prevención & control , Proteínas de la Membrana/clasificación , Proteínas de la Membrana/inmunología , Factores de Riesgo , Ovinos , Enfermedades de las Ovejas/genética , Enfermedades de las Ovejas/inmunología , Enfermedades de las Ovejas/virologíaRESUMEN
The env gene in Small Ruminant Lentiviruses (SRLV) encodes the surface glycoprotein (SU) that divides into conserved (C1-C4) and variable regions (V1-V5). SRLV region V4 has been found to be homologous to the V3 region of human lentivirus (HIV). HIV V3 is responsible for tropism and the development of nervous clinical patterns when there is a tendency to conserve amino acids in specific "signature pattern" positions. The goal of this study was to identify signature patterns in the V4 region of the SU, which is encoded by the SRLV env gene. Secondarily, to understand how these signature patterns are associated with different clinical status in naturally infected sheep and goats. Starting with 244 samples from seropositive animals from nine Mexican states, we amplified the V4 region using nested PCR and obtained 49 SRLV sequences from peripheral blood leukocytes. Based on phylogenetic analysis results, we identified three groups: asymptomatic genotypes A (Ssx GA) and B (Ssx GB), as well as animals with arthritic presentation, genotype B (A GB). Similarity levels between group sequences ranged from 67.9%-86.7%, with a genetic diversity ranging from 12.7%-29.5% and a dN / dS ratio that indicated negative selection. Analyses using Vespa and Entropy programs identified four residues at positions 54, 78, 79 and 82 in SU region V4 as possible signature patterns, although with variable statistical significance. However, position 54 residues "N" (p = 0.017), "T" (p = 0.001) and "G" (p = 0.024) in groups A GB, Ssx GA and Ssx GB respectively, best characterized the signature patterns. The results obtained identified a signature pattern related to different genotypes and clinical status by SRLV in sheep and goats.
Asunto(s)
Variación Genética , Infecciones por Lentivirus/veterinaria , Lentivirus/genética , Proteínas del Envoltorio Viral/genética , Animales , Infecciones Asintomáticas , Femenino , Genotipo , Enfermedades de las Cabras/virología , Cabras , Lentivirus/clasificación , Infecciones por Lentivirus/virología , Masculino , Filogenia , Análisis de Secuencia de ADN , Ovinos , Enfermedades de las Ovejas/virología , TranscriptomaRESUMEN
Small ruminant lentiviruses (SRLVs) are found in sheep in Germany and Iran. SRLVs have been classified into four genotypes: A-C and E. Genotype A has been subdivided into 20 subtypes. Previous studies suggested that, first, the ancestors of genotype A are those SRLVs found in Turkey, second, the evolution of SRLVs is related to the domestication process, and, third, SRLV infection was first observed in sheep in Iceland and the source of that infection was a flock imported from Germany. This study generated, for the first time, partial SRLV sequence data from German and Iranian sheep, enhancing our knowledge of the genetic and evolutionary relationships of SRLVs, and their associations with the domestication process. Based on 54 SRLV sequences from German and Iranian sheep, our results reveal: (1) SRLV subtypes A4, A5, A11, A16 and A21 (new) are found in German sheep and A22 (new) in Iranian sheep. (2) Genotype A has potentially an additional ancestor (A22), found in Iran, Lebanon and Jordan. (3) Subtype A22 is likely an old version of SRLVs. (4) The transmission routes of some SRLVs are compatible with domestication pathways. (5) This study found no evidence of Icelandic subtype A1 in German sheep.
Asunto(s)
Infecciones por Lentivirus , Lentivirus/clasificación , Lentivirus/aislamiento & purificación , Filogenia , Enfermedades de las Ovejas , Oveja Doméstica/virología , Animales , Asia , Domesticación , Europa (Continente) , Infecciones por Lentivirus/transmisión , Infecciones por Lentivirus/veterinaria , Infecciones por Lentivirus/virología , Ovinos , Enfermedades de las Ovejas/transmisión , Enfermedades de las Ovejas/virologíaRESUMEN
Lentiviral replication mediated by reverse transcriptase is considered to be highly error prone, leading to a high intra-individual evolution rate that promotes evasion of neutralization and persistent infection. Understanding lentiviral intra-individual evolutionary dynamics on a comparative basis can therefore inform research strategies to aid in studies of pathogenesis, vaccine design, and therapeutic intervention. We conducted a systematic review of intra-individual evolution rates for three species groups of lentiviruses-feline immunodeficiency virus (FIV), simian immunodeficiency virus (SIV), and human immunodeficiency virus (HIV). Overall, intra-individual rate estimates differed by virus but not by host, gene, or viral strain. Lentiviral infections in spillover (nonadapted) hosts approximated infections in primary (adapted) hosts. Our review consistently documents that FIV evolution rates within individuals are significantly lower than the rates recorded for HIV and SIV. FIV intra-individual evolution rates were noted to be equivalent to FIV interindividual rates. These findings document inherent differences in the evolution of FIV relative to that of primate lentiviruses, which may signal intrinsic difference of reverse transcriptase between these viral species or different host-viral interactions. Analysis of lentiviral evolutionary selection pressures at the individual versus population level is valuable for understanding transmission dynamics and the emergence of virulent and avirulent strains and provides novel insight for approaches to interrupt lentiviral infections.IMPORTANCE To the best of our knowledge, this is the first study that compares intra-individual evolution rates for FIV, SIV, and HIV following systematic review of the literature. Our findings have important implications for informing research strategies in the field of intra-individual virus dynamics for lentiviruses. We observed that FIV evolves more slowly than HIV and SIV at the intra-individual level and found that mutation rates may differ by gene sequence length but not by host, gene, strain, an experimental setting relative to a natural setting, or spillover host infection relative to primary host infection.
Asunto(s)
Evolución Biológica , Interacciones Huésped-Patógeno , Infecciones por Lentivirus/virología , Lentivirus/fisiología , Animales , Gatos , Evolución Molecular , Síndrome de Inmunodeficiencia Adquirida del Felino/virología , Variación Genética , VIH/genética , Infecciones por VIH/virología , Humanos , Virus de la Inmunodeficiencia Felina/genética , Lentivirus/clasificación , Primates , Síndrome de Inmunodeficiencia Adquirida del Simio/virología , Virus de la Inmunodeficiencia de los Simios/genéticaRESUMEN
Small-ruminant lentivirus (SRLV) infections are widespread in Poland, and circulation of subtypes A1, A12, A13, A16, A17, B1 and B2 has been documented. The aim of this study was to characterize the SRLV strains circulating in sheep and goats in mixed flocks in the Malopolska region, where the highest seroprevalence has been detected. Phylogenetic analysis revealed that most of the isolates from sheep belonged to subtype A13, suggesting that this subtype may be predominant in the Malopolska region. Furthermore, the existence of a new subtype, tentatively designated as A18, was described for the first time. This work extends the current knowledge on the distribution of SRLV subtypes in sheep and goats in Poland and provides further information on the genetic diversity of SRLV. The new data are important for both epidemiological studies and eradication programs and provide insight into the evolution of SRLV.
Asunto(s)
Enfermedades de las Cabras/virología , Infecciones por Lentivirus/veterinaria , Lentivirus/genética , Lentivirus/aislamiento & purificación , Enfermedades de las Ovejas/virología , Secuencia de Aminoácidos , Animales , Productos del Gen gag/química , Productos del Gen gag/genética , Cabras , Lentivirus/química , Lentivirus/clasificación , Infecciones por Lentivirus/virología , Datos de Secuencia Molecular , Filogenia , Polonia , Alineación de Secuencia , OvinosRESUMEN
INTRODUCTION: Small-ruminant lentivirus (SRLV) infection is widespread across Europe. It causes substantial economic losses in sheep breeding. The main route of SRLV infection is through the mother's milk, especially colostrum However, infection can also occur via contact between infected and healthy animals. It should be noted that the mechanisms of contact infection are still relatively poorly understood. The virus can also spread through a flock via an aerogenic mechanism. OBJECTIVE: Due to the increased risk of SRLV infection in sheep bred in an alcove system, this study sought to define the effect of various selected factors associated with alcove breeding on the frequency of SRLV infection in sheep. MATERIAL AND METHODS: Risk factors associated with small-ruminant lentivirus (SRLV) infection were analyzed among flocks of sheep in central-eastern Poland. Ninety-eight sheep flocks were selected for detailed investigation and included 6,470 ewes and 15 breeds and lines. Serologic testing of blood samples was used to identify infected animals and evaluate the epidemiologic status of particular flocks. Specific antibodies for Maedi Visna Virus (MVV) were detected via ELISA. Questionnaires were used to gather information concerning risk factors. RESULTS: The study's results indicate that factors associated with environmental conditions under which sheep are kept play a significant role in determining the risk of SRLV infection. CONCLUSIONS: Special attention should be focused on airborne contamination associated with the technologies used in sheep breeding. Breeding technologies that limit airborne contamination in farm buildings should be employed. In developing programmes to eliminate SLRV in sheep flocks, improvement of zoohygenic conditions should also be considered.
Asunto(s)
Infecciones por Lentivirus/veterinaria , Lentivirus/aislamiento & purificación , Enfermedades de las Ovejas/virología , Crianza de Animales Domésticos/instrumentación , Animales , Anticuerpos Antivirales/sangre , Ensayo de Inmunoadsorción Enzimática , Europa (Continente)/epidemiología , Femenino , Vivienda para Animales , Lentivirus/clasificación , Lentivirus/genética , Lentivirus/fisiología , Infecciones por Lentivirus/sangre , Infecciones por Lentivirus/epidemiología , Infecciones por Lentivirus/virología , Masculino , Factores de Riesgo , Ovinos , Enfermedades de las Ovejas/diagnóstico , Enfermedades de las Ovejas/epidemiologíaRESUMEN
Small Ruminant Lentiviruses (SRLVs) are widespread in many countries and cause economically relevant, slow, and persistent diseases in sheep and goats. Monitoring the genetic diversity of SRLVs is useful to improve the diagnostic tools used in the eradication programs. In this study, SRLVs detected in Spanish Assaf sheep with different grades of lymphoproliferative mastitis were sequenced. Genetic characterization showed that most samples belonged to type A and were closer to Spanish SRLV isolates previously classified as A2/A3. Four samples belonged to subtype B2 and showed higher homology with Italian B2 strains than with Spanish B2 isolates. Amino acid sequences of immuno-dominant epitopes in the gag region were very conserved while more alterations were found in the LTR sequences. No significant correlations were found between grades of mastitis and alterations in the sequences although samples with similar histological features were phylogenetically closer to each other. Broader genetic characterization surveys in samples with different grades of SRLV-lesions are required for evaluating potential correlations between SRLV sequences and the severity of diseases.
Asunto(s)
Variación Genética , Infecciones por Lentivirus/veterinaria , Lentivirus/clasificación , Lentivirus/aislamiento & purificación , Glándulas Mamarias Animales/patología , Enfermedades de las Ovejas/virología , Animales , Genotipo , Lentivirus/genética , Infecciones por Lentivirus/patología , Infecciones por Lentivirus/virología , Filogenia , Análisis de Secuencia de ADN , Homología de Secuencia , Ovinos , Enfermedades de las Ovejas/patología , EspañaRESUMEN
BACKGROUND: The apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3; A3) gene family appears only in mammalian genomes. Some A3 proteins can be incorporated into progeny virions and inhibit lentiviral replication. In turn, the lentiviral viral infectivity factor (Vif) counteracts the A3-mediated antiviral effect by degrading A3 proteins. Recent investigations have suggested that lentiviral vif genes evolved to combat mammalian APOBEC3 proteins, and have further proposed that the Vif-A3 interaction may help determine the co-evolutionary history of cross-species lentiviral transmission in mammals. RESULTS: Here we address the co-evolutionary relationship between two New World felids, the puma (Puma concolor) and the bobcat (Lynx rufus), and their lentiviruses, which are designated puma lentiviruses (PLVs). We demonstrate that PLV-A Vif counteracts the antiviral action of APOBEC3Z3 (A3Z3) of both puma and bobcat, whereas PLV-B Vif counteracts only puma A3Z3. The species specificity of PLV-B Vif is irrespective of the phylogenic relationships of feline species in the genera Puma, Lynx and Acinonyx. We reveal that the amino acid at position 178 in the puma and bobcat A3Z3 is exposed on the protein surface and determines the sensitivity to PLV-B Vif-mediated degradation. Moreover, although both the puma and bobcat A3Z3 genes are polymorphic, their sensitivity/resistance to PLV Vif-mediated degradation is conserved. CONCLUSIONS: To the best of our knowledge, this is the first study suggesting that the host A3 protein potently controls inter-genus lentiviral transmission. Our findings provide the first evidence suggesting that the co-evolutionary arms race between lentiviruses and mammals has occurred in the New World.
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Citosina Desaminasa/genética , Interacciones Huésped-Patógeno/genética , Infecciones por Lentivirus/transmisión , Infecciones por Lentivirus/virología , Lentivirus/fisiología , Animales , Gatos , Citosina Desaminasa/química , Citosina Desaminasa/metabolismo , Resistencia a la Enfermedad , Evolución Molecular , Productos del Gen vif , Virus de la Inmunodeficiencia Felina/clasificación , Virus de la Inmunodeficiencia Felina/genética , Lentivirus/clasificación , Mutación con Pérdida de Función , Modelos Moleculares , Filogenia , Polimorfismo Genético , Conformación Proteica , Proteolisis , Relación Estructura-Actividad , Treonina/química , Treonina/genéticaRESUMEN
Divergence date estimates are central to understand evolutionary processes and depend, in the case of molecular phylogenies, on tests of molecular clocks. Here we propose two non-parametric tests of strict and relaxed molecular clocks built upon a framework that uses the empirical cumulative distribution (ECD) of branch lengths obtained from an ensemble of Bayesian trees and well known non-parametric (one-sample and two-sample) Kolmogorov-Smirnov (KS) goodness-of-fit test. In the strict clock case, the method consists in using the one-sample Kolmogorov-Smirnov (KS) test to directly test if the phylogeny is clock-like, in other words, if it follows a Poisson law. The ECD is computed from the discretized branch lengths and the parameter λ of the expected Poisson distribution is calculated as the average branch length over the ensemble of trees. To compensate for the auto-correlation in the ensemble of trees and pseudo-replication we take advantage of thinning and effective sample size, two features provided by Bayesian inference MCMC samplers. Finally, it is observed that tree topologies with very long or very short branches lead to Poisson mixtures and in this case we propose the use of the two-sample KS test with samples from two continuous branch length distributions, one obtained from an ensemble of clock-constrained trees and the other from an ensemble of unconstrained trees. Moreover, in this second form the test can also be applied to test for relaxed clock models. The use of a statistically equivalent ensemble of phylogenies to obtain the branch lengths ECD, instead of one consensus tree, yields considerable reduction of the effects of small sample size and provides a gain of power.
Asunto(s)
Evolución Molecular , Modelos Genéticos , Filogenia , Animales , Ascomicetos/clasificación , Ascomicetos/genética , Teorema de Bayes , Simulación por Computador , Ciclooxigenasa 1/genética , ADN/genética , Bases de Datos Genéticas , Productos del Gen env/genética , Humanos , Lentivirus/clasificación , Lentivirus/genética , Distribución de Poisson , Primates/clasificación , Primates/genética , Proteínas/genética , Estadísticas no Paramétricas , Factores de TiempoRESUMEN
The HIV-1 RNA genome contains complex structures with many structural elements playing regulatory roles during viral replication. A recent study has identified multiple RNA structures with unknown functions that are conserved among HIV-1 and two simian immunodeficiency viruses. To explore the roles of these conserved RNA structures, we introduced synonymous mutations into the HIV-1 genome to disrupt each structure. These mutants exhibited similar particle production, viral infectivity, and replication kinetics relative to the parent NL4-3 virus. However, when replicating in direct competition with the wild-type NL4-3 virus, mutations of RNA structures at inter-protein domain junctions can cause fitness defects. These findings reveal the ability of HIV-1 to tolerate changes in its sequences, even in apparently highly conserved structures, which permits high genetic diversity in HIV-1 population. Our results also suggest that some conserved RNA structures may function to fine-tune viral replication.
Asunto(s)
VIH-1/genética , Lentivirus/genética , ARN Viral/química , Secuencia de Bases , Secuencia Conservada , Infecciones por VIH/virología , VIH-1/química , VIH-1/fisiología , Humanos , Secuencias Invertidas Repetidas , Lentivirus/química , Lentivirus/clasificación , Lentivirus/fisiología , Infecciones por Lentivirus/virología , Conformación de Ácido Nucleico , ARN Viral/genética , ARN Viral/metabolismo , Replicación ViralRESUMEN
Viruses have evolved specialized molecular mechanisms to transfer their genome efficiently into host cells. Viruses can be repurposed into viral vectors to achieve controlled gene transfer to desired cells. One of the most popular classes of vectors, lentiviral vectors (LVs), transduce mammalian cells efficiently. LVs are pseudotyped with various heterologous viral envelopes to alter their tropism. While the most common example is the envelope glycoprotein from vesicular stomatitis virus (VSVG), many other viral proteins have also been used. Pseudotyping LVs with a diverse set of naturally occurring or engineered viral envelopes has allowed targeted transduction of specific cell types. Many exciting studies are further uncovering new specificities and shortcomings of pseudotyped LVs. These studies will expand the toolbox to make LVs that cater to the specific requirements of transduction. This review provides a comprehensive overview of various viral envelope pseudotypes used with LVs, their specificities, advantages, and drawbacks.
Asunto(s)
Vectores Genéticos/genética , Lentivirus/genética , Transducción Genética/métodos , Terapia Genética/métodos , Lentivirus/clasificación , Lentivirus/fisiología , Proteínas del Envoltorio Viral/genética , Proteínas del Envoltorio Viral/metabolismoRESUMEN
G-quadruplexes (G4s) are secondary structures of nucleic acids that epigenetically regulate cellular processes. In the human immunodeficiency lentivirus 1 (HIV-1), dynamic G4s are located in the unique viral LTR promoter. Folding of HIV-1 LTR G4s inhibits viral transcription; stabilization by G4 ligands intensifies this effect. Cellular proteins modulate viral transcription by inducing/unfolding LTR G4s. We here expanded our investigation on the presence of LTR G4s to all lentiviruses. G4s in the 5'-LTR U3 region were completely conserved in primate lentiviruses. A G4 was also present in a cattle-infecting lentivirus. All other non-primate lentiviruses displayed hints of less stable G4s. In primate lentiviruses, the possibility to fold into G4s was highly conserved among strains. LTR G4 sequences were very similar among phylogenetically related primate viruses, while they increasingly differed in viruses that diverged early from a common ancestor. A strong correlation between primate lentivirus LTR G4s and Sp1/NFκB binding sites was found. All LTR G4s folded: their complexity was assessed by polymerase stop assay. Our data support a role of the lentiviruses 5'-LTR G4 region as control centre of viral transcription, where folding/unfolding of G4s and multiple recruitment of factors based on both sequence and structure may take place.
Asunto(s)
Secuencia Conservada , G-Cuádruplex , Lentivirus/genética , Regiones Promotoras Genéticas , Secuencias Repetidas Terminales , Animales , Secuencia de Bases , Sitios de Unión , Duplicado del Terminal Largo de VIH , VIH-1/genética , Humanos , Lentivirus/clasificación , Filogenia , Posición Específica de Matrices de Puntuación , Unión Proteica , Factor de Transcripción Sp1/metabolismoRESUMEN
Small ruminant lentiviruses (SRLV) globally affect welfare and production of sheep and goats and are mainly controlled through elimination of infected animals, independently of the viral kinetics within the single animal. Control programs are based on highly sensitive serological tests, however the existence of low antibody responders leads to the permanent presence of seronegative infected animals in the flock, thus perpetuating the infection. On the other hand, long-term non-progressors show a detectable antibody response not indicative of a shedding animal, suggesting immune contention of infection. In this study, we analyse two goat populations within the same herd, harbouring low or high proviral SRLV loads respectively, both showing a robust antibody response. In vivo findings were confirmed in vitro since fibroblastic cell lines obtained from one high and one low proviral load representative goats, showed respectively a high and a faint production of virus upon infection with reference and field circulating SRLV strains. Differences in virus production were relieved when strain CAEV-Co was used for experimental infection. We analysed LTR promoter activity, proviral load, entry step and production of virus and viral proteins. Intriguingly, proteasomal activity was higher in fibroblasts from low proviral load animals and proteasome inhibition increased viral production in both cell lines, suggesting the implication of active proteasome-dependent restriction factors. Among them, we analysed relative expression and sequences of TRIM5α, APOBEC3 (Z1, Z2, Z3 and Z2-Z3) and BST-2 (Tetherin) and found a global antiviral status in low proviral carriers that may confer protection against viral shedding and disease onset.
Asunto(s)
Enfermedades de las Cabras/virología , Infecciones por Lentivirus/veterinaria , Lentivirus/clasificación , Desaminasas APOBEC/genética , Desaminasas APOBEC/metabolismo , Animales , Biomarcadores , Línea Celular , ADN Viral/genética , Ensayo de Inmunoadsorción Enzimática/veterinaria , Fibroblastos/metabolismo , Fibroblastos/virología , Regulación de la Expresión Génica , Predisposición Genética a la Enfermedad , Cabras , Humanos , Lentivirus/genética , Infecciones por Lentivirus/virología , Provirus , Purinas , Proteínas Virales/genética , Proteínas Virales/metabolismo , Internalización del Virus , Esparcimiento de VirusRESUMEN
The major challenges in diagnosing small ruminant lentivirus (SRLV) infection include early detection and genotyping of strains of epidemiological interest. A longitudinal study was carried out in Rasa Aragonesa sheep experimentally infected with viral strains of genotypes A or B from Spanish neurological and arthritic SRLV outbreaks, respectively. Sera were tested with two commercial ELISAs, three based on specific peptides and a novel combined peptide ELISA. Three different PCR assays were used to further assess infection status. The kinetics of anti-viral antibody responses were variable, with early diagnosis dependent on the type of ELISA used. Peptide epitopes of SRLV genotypes A and B combined in the same ELISA well enhanced the overall detection rate, whereas single peptides were useful for genotyping the infecting strain (A vs. B). The results of the study suggest that a combined peptide ELISA can be used for serological diagnosis of SRLV infection, with single peptide ELISAs useful for subsequent serotyping.
Asunto(s)
Ensayo de Inmunoadsorción Enzimática/veterinaria , Infecciones por Lentivirus/veterinaria , Lentivirus/genética , Péptidos/química , Enfermedades de las Ovejas/virología , Animales , Anticuerpos Antivirales/sangre , Genotipo , Lentivirus/clasificación , Infecciones por Lentivirus/diagnóstico , Infecciones por Lentivirus/virología , Masculino , Reacción en Cadena de la Polimerasa/veterinaria , Pruebas Serológicas , Ovinos , Enfermedades de las Ovejas/diagnósticoRESUMEN
BACKGROUND: A significant fraction of mammalian genomes is composed of endogenous retroviral (ERV) sequences that are formed by germline infiltration of various retroviruses. In contrast to other retroviral genera, lentiviruses only rarely form ERV copies. We performed a computational search aimed at identification of novel endogenous lentiviruses in vertebrate genomes. FINDINGS: Using the in silico strategy, we have screened 104 publicly available vertebrate genomes for the presence of endogenous lentivirus sequences. In addition to the previously described cases, the search revealed the presence of endogenous lentivirus in the genome of Malayan colugo (Galeopterus variegatus). At least three complete copies of this virus, denoted ELVgv, were detected in the colugo genome, and approximately one hundred solo LTR sequences. The assembled consensus sequence of ELVgv had typical lentivirus genome organization including three predicted accessory genes. Phylogenetic analysis placed this virus as a distinct subgroup within the lentivirus genus. The time of insertion into the dermopteran lineage was estimated to be more than thirteen million years ago. CONCLUSIONS: We report the discovery of the first endogenous lentivirus in the mammalian order Dermoptera, which is a taxon close to the Primates. Lentiviruses have infiltrated the mammalian germline several times across millions of years. The colugo virus described here represents possibly the oldest documented endogenization event and its discovery can lead to new insights into lentivirus evolution. This is also the first report of an endogenous lentivirus in an Asian mammal, indicating a long-term presence of this retrovirus family in Asian continent.