RESUMEN
Multifunctional probes are needed to characterize individual cells simultaneously by different techniques to provide complementary information. A preparative method and an in vitro demonstration of function are presented for a dual-function dark field microscopy/surface-enhanced Raman scattering (SERS) liposome probe for cancer. Liposomes composed of zwitterionic lipids are valuable both to limit biofouling and to serve as a modular matrix to incorporate a variety of functional molecules and hence are used here as vehicles for SERS-active materials. Dark field microscopy and SERS represent new combined functionalities for targeted liposomal probes. Two methods of antibody conjugation to SERS liposomes are demonstrated: (i) direct conjugation to functional groups on the SERS liposome surface and (ii) postinsertion of lipid-functionalized antibody fragments (Fabs) into preformed SERS liposomes. In vitro experiments targeting both lymphoma cell line LY10 and primary human chronic lymphocytic leukemia (CLL) cells demonstrate the usefulness of these probes as optical contrast agents in both dark field and Raman microscopy.
Asunto(s)
Leucemia de Células B/diagnóstico por imagen , Liposomas/química , Linfoma/diagnóstico por imagen , Animales , Anticuerpos/inmunología , Línea Celular Tumoral , Colesterol/química , Cabras , Oro/química , Humanos , Leucemia de Células B/inmunología , Linfoma/inmunología , Nanopartículas del Metal/química , Fosfatidilcolinas/química , Ovinos , Espectrometría Raman/métodos , Esfingomielinas/químicaRESUMEN
A 47-year-old man presented with abdominal pain, vomiting, and bone pain. Laboratory findings revealed severe hypercalcemia, anemia, and renal insufficiency with decreased serum parathyroid hormone. FDG PET/CT was performed for characteristics suggestive of multiple myeloma and other occult malignancy. The images revealed widespread osteolytic lesions with only 1 focus of definite abnormal FDG uptake. B-cell acute lymphoblastic leukemia was confirmed by pathological examination following bone marrow biopsy.
Asunto(s)
Fluorodesoxiglucosa F18 , Leucemia de Células B/complicaciones , Leucemia de Células B/diagnóstico por imagen , Osteólisis/complicaciones , Tomografía Computarizada por Tomografía de Emisión de Positrones , Transporte Biológico , Biopsia , Fluorodesoxiglucosa F18/metabolismo , Humanos , Leucemia de Células B/metabolismo , Leucemia de Células B/patología , Masculino , Persona de Mediana Edad , Mieloma Múltiple/complicacionesAsunto(s)
Mama/diagnóstico por imagen , Leucemia de Células B/patología , Infiltración Leucémica/diagnóstico por imagen , Tomografía de Emisión de Positrones , Tomografía Computarizada por Rayos X , Mama/patología , Femenino , Fluorodesoxiglucosa F18 , Humanos , Leucemia de Células B/diagnóstico por imagen , Imagen Multimodal , Radiofármacos , Adulto JovenRESUMEN
Pancreatic infiltration with leukemic cells is a rare manifestation of acute lymphoblastic leukemia. There are only a few reported cases. We report the clinical and radiologic findings of a 4-year-old boy with mature B-cell acute lymphoblastic leukemia and pancreatic involvement. A computed tomography scan of his abdomen demonstrated diffuse hypodense lesions in the pancreas. Plasma amylase and lipase levels at that time were high, but no signs of hypoglycemia or hyperglycemia were observed. After 2 cycles of chemotherapy, the lesions in his pancreas, liver, and kidney disappeared, and his pancreatitis resolved as well. At 11 months' follow-up, after completion of therapy, the patient continues to be in remission.
