RESUMEN
Metarhizium anisopliae is an important class of entomopathogenic fungi used for the biocontrol of insects, but its virulence is affected by insect immunity. We identified a novel FK506 binding protein gene that was differentially expressed between control and Metarhizium-treated Locusta migratoria manilensis. We hypothesized that this protein played an important role in Metarhizium infection of L. migratoria and could provide new insights for developing highly efficient entomopathogenic fungi. We, therefore, cloned the specific gene and obtained its purified protein. The gene was then named FKBP52, and its dsRNA (dsFKBP52) was synthesized and used for gene interference. Bioassay results showed that the mortality of L. migratoria treated with dsFKBP52â +â Metarhizium was significantly lower than that of other treatments. Furthermore, immune-related genes (MyD88, Dorsal, Cactus, and Defensin) in L. migratoria treated with dsFKBP52â +â Metarhizium showed significant upregulation compared to that treated with Metarhizium only. However, the activities of peroxidase (POD), superoxide dismutase (SOD), and calcineurin (CaN) showed fluctuations. These results suggest that the FKBP52 gene may play a crucial role in the innate immunity of L. migratoria. The effect of its silencing indicated that this immunity-related protein might be a potential target for insect biocontrol.
Asunto(s)
Proteínas de Insectos , Locusta migratoria , Metarhizium , Proteínas de Unión a Tacrolimus , Animales , Locusta migratoria/genética , Locusta migratoria/inmunología , Metarhizium/fisiología , Metarhizium/genética , Proteínas de Unión a Tacrolimus/genética , Proteínas de Insectos/genética , Proteínas de Insectos/metabolismo , Control Biológico de Vectores , Inmunidad Innata , Secuencia de AminoácidosRESUMEN
The timing and mechanism by which a host insect initiates an immune response are critical to successful defense against infection. Pathogen recognition, a prerequisite for host defense, has long been recognized to take place during the insect epidermis invasion by fungus. Here we report that insect can sense the fungal pathogen before host cuticle is penetrated by fungus. We discovered the upstream pattern recognition receptor (PRR) genes of the Toll pathway were upregulated in both the integument and fat body early during fungal germination on the epicuticle of Locusta migratoria manilensis. The Toll signaling pathway was strongly activated in the fat body at the penetration stage. RNAi of Myd88 increased the susceptibility of locusts to fungal infection, but that of Cactus showed the opposite effect. In addition, ß-1, 3-glucan (laminarin), the main component of the cell wall of the pathogenic fungus Metarhizium acridum, was capable of activating the Toll signaling pathway (Spaetzle and Cactus) when it was applied on the host cuticle. These results demonstrate that host epidemis can effectively defend fungal infection by detecting ß-1, 3-glucan on the fungal cell wall and activate the Toll signaling pathway even before fungal penetration.
Asunto(s)
Enfermedades de los Peces/inmunología , Proteínas de Insectos/genética , Locusta migratoria/inmunología , Metarhizium/fisiología , Micosis/inmunología , Proteoglicanos/metabolismo , Receptores de Reconocimiento de Patrones/genética , Receptores Toll-Like/genética , Animales , Pared Celular/inmunología , Proteínas de Unión al ADN/genética , Resistencia a la Enfermedad , Proteínas de Drosophila/genética , Proteínas de Insectos/metabolismo , Fosfoproteínas/genética , Receptores de Reconocimiento de Patrones/metabolismo , Transducción de Señal , Receptores Toll-Like/metabolismo , Regulación hacia ArribaRESUMEN
Immune response and reproductive success are two vital energy-consuming processes in living organisms. However, it is still unclear which process is prioritized when both are required. Therefore, the present study was designed to examine this question arising for one of the world's most destructive agricultural pests, the migratory locust, Locusta migratoria. Transcripts from the ovaries and fat bodies of newly emerged locusts were analyzed, using RNA-seq based transcriptome and qualitative real-time PCR, at 4 h and 6 d after being infected with the gram-positive bacteria Micrococcus luteus. Changes in the main biological pathways involved in reproduction and immunization were analyzed using bioinformatics. After 4 h of infection, 348 and 133 transcripts were up- and down-regulated, respectively, whereas 5699 and 44 transcripts were up- and down-regulated, respectively, at 6 d after infection. Moreover, KEGG analysis indicated that vital pathways related with immunity and reproduction, such as Insulin resistance, FoxO signaling, Lysosome, mTOR signaling, and Toll-like receptor signaling pathways were up-regulated. Among the differentially expressed genes, 22 and 17 were related to immunity and reproduction, respectively. The expression levels of PPO1 and antimicrobial peptide defensin 3 were increased (log2FC = 5.93 and 6.75, respectively), whereas those of VgA and VgB were reduced (log2FC = -17.82 and -18.13, respectively). These results indicated that locust allocate energy and resources to maintain their own survival by increasing immune response when dealing with both immune and reproductive processes. The present study provides the first report of expression levels for genes related with reproduction and immunity in locusts, thereby providing a reference for future studies, as well as theoretical guidance for investigations of locust control.
Asunto(s)
Inmunidad Innata/genética , Proteínas de Insectos/genética , Locusta migratoria/genética , Micrococcus luteus/fisiología , Reproducción/genética , Transcriptoma , Animales , Biología Computacional , Regulación de la Expresión Génica , Locusta migratoria/inmunología , Locusta migratoria/microbiología , Transducción de SeñalRESUMEN
BACKGROUND: The migratory locust Locusta migratoria is one of the most important agricultural pests worldwide. The nuclear export factor 1 (NXF1) protein plays a crucial role in mediating mRNA transport from the nucleus to the cytoplasm. This study evaluates whether NXF1 could be a potential target for RNAi-mediated pest control of L. migratoria. RESULTS: We cloned and characterized the nuclear export factor lm-nxf1 of L. migratoria. Lm-nxf1 was expressed in all tissues examined, including head, fat body, hemolymph, trunk, leg and midgut, with high expression observed in the hemolymph and fat body. Injection of lm-nxf1 dsRNA into hemolymph resulted in inhibition of mRNA export in hemocytes, which were used as a target for observing mRNA export. Total hemocyte levels were reduced by ca. 97% in lm-nxf1-dsRNA-treated locusts, and high insect mortality occurred with LT50 = 7.75 day as compared with 18.15 day for gfp-dsRNA-treated controls. Further, the locust intestine became atrophy, and the opportunistic pathogens Enterobacter aerogenes, Klebsiella pneumoniae and Enterobacter asburiae were specifically detected in midgut after lm-nxf1 dsRNA treatment. CONCLUSIONS: The results reveal that knockdown of the lm-nxf1 gene affects the survival of L. migratoria, indicating that lm-nxf1 is a potential target for RNAi-mediated pest control. © 2018 Society of Chemical Industry.
Asunto(s)
Microbioma Gastrointestinal/genética , Técnicas de Silenciamiento del Gen , Locusta migratoria/genética , Locusta migratoria/microbiología , Proteínas de Transporte Nucleocitoplasmático/deficiencia , Proteínas de Transporte Nucleocitoplasmático/genética , Interferencia de ARN , Animales , Transporte Biológico/genética , Regulación de la Expresión Génica/genética , Hemocitos/citología , Locusta migratoria/inmunología , Locusta migratoria/fisiología , Proteínas de Transporte Nucleocitoplasmático/metabolismo , ARN Mensajero/metabolismoRESUMEN
20-hydroxyecdysone (20E) has been implicated in regulating the immune response in insects. Conflicting conclusions on 20E regulating immunity have been reported in model holometabolous species. However, in hemimetabolous insects, the role of 20E as an immune-suppressor or activator and the mechanism remains unclear. The migratory locust Locusta migratoria is a representative member of hemimetabolous insects. Here, digital gene expression (DGE) profiles of Locusta migratoria treated with 20E were analyzed. Pattern recognition receptors [peptidoglycan recognition protein (PGRP-SA), PGRP-LE, and gram-negative binding protein (GNBP3)] and antimicrobial peptides (defensin, diptericin, and i-type lysozyme) were significantly induced by 20E in fat body. These immune-related genes significantly increased their mRNA levels during the high-20E stage. Antibacterial activities in plasma were enhanced after 20E injection and during the high-20E developmental stage. Conversely, when 20E signal was suppressed by RNAi of EcR (ecdysone receptor), the expression levels of these genes and antibacterial activities failed to be increased by 20E injection and during the high-20E developmental stage, and the mortality increased after being infected by entomogenous fungus. The knockdown of PGRP-SA inhibited the expression level of defensin, diptericin and i-type lysozyme in fat body and reduced antibacterial activities in plasma. 20E injection could not significantly induce the expression of antimicrobial peptides after RNAi of PGRP-SA. These results demonstrated that 20E enhanced the immune response by activating PGRP-SA in L. migratoria.
Asunto(s)
Infecciones Bacterianas/inmunología , Proteínas Portadoras/metabolismo , Ecdisterona/inmunología , Escherichia coli/inmunología , Proteínas de Insectos/metabolismo , Locusta migratoria/inmunología , Receptores de Esteroides/metabolismo , Staphylococcus aureus/inmunología , Animales , Péptidos Catiónicos Antimicrobianos/metabolismo , Células Cultivadas , Clonación Molecular , Regulación del Desarrollo de la Expresión Génica , Inmunidad Innata , Micosis/inmunología , Ninfa , ARN Interferente Pequeño/genética , Receptores de Esteroides/genéticaRESUMEN
Hemocytes are the first line of defense in the invertebrate immune system. Understanding their roles in cellular immunity is important for developing more efficient mycoinsecticides. However, the exact classification of hemocytes has been inconsistent and the various types of phagocytes in Locusta migratoria are poorly defined. Herein, the Wright-Giemsa staining method and microscopy were employed to characterize the hemocytes of L. migratoria following infection by Metarhizium acridum. Hemocytes were classified into four types, including granulocytes, plasmatocytes, prohemocytes, and oenocytoids, based on size, morphology, and dye-staining properties. Each type of hemocyte was classified into several subtypes according to different ultrastructural features. At least four subtypes of granulocytes or plasmatocytes, including small-nucleus plasmatocytes, basophil vacuolated plasmatocytes, homogeneous plasmatocytes, and eosinophilic granulocytes, carried out phagocytosis. The percentage of total phagocytes increased two days after infection by M. acridum, then gradually declined during the next two days, and then increased sharply again at the fifth day. Our data suggested that plasmatocytes and granulocytes may be the major phagocytes that protect against invasion by a fungal pathogen in L. migratoria. Total hemocytes in locusts significantly increased in the initial days after infection and decreased in the late period of infection compared to controls. In the hemocoel, hyphal bodies were recognized, enwrapped, and digested by the phagocytes. Then, the broken hyphal pieces were packaged as vesicles to be secreted from the cell. Moreover, locusts might have a sensitive and efficient cellular immune system that can regulate phagocyte differentiation and proliferation before fungi colonize the host hemolymph.
Asunto(s)
Hemocitos/clasificación , Locusta migratoria/inmunología , Fagocitos/clasificación , Coloración y Etiquetado/métodos , Animales , Hemocitos/citología , Locusta migratoria/citología , Locusta migratoria/parasitología , Masculino , Metarhizium , Micosis/veterinaria , Fagocitos/citologíaRESUMEN
BACKGROUND: Metarhizium is an important class of entomopathogenic fungi in the biocontrol of insects, but its virulence is affected by insect immunity. To clarify the mechanism in virulence of Metarhizium, we compared the immunological differences in Locusta migratoria L. when exposed to two strains of Metarhizium anisopliae (Ma). RESULTS: The virulence of Ma IMI330189 was significantly higher than that of Ma IBC200614 to locust, and IMI330189 overcame the hemocytes and began destroying the hemocytes of locust at 72 h after spray, while locust is immune to IBC200614. IMI330189 could overcome the humoral immunity of locust by inhibiting the activities of phenol oxidase (PO), esterases, multi-function oxidases (MFOs) and acetylcholinesterases in locust while increasing the activities of glutathione-S-transferases (GSTs), catalase and aryl-acylamidase (AA). However IBC200614 inhibit the activities of GSTs and AA in locust and increase the activities of MFOs, PO, superoxide dismutase, peroxidase and chitinase in locust. The changes of enzymes activities in period of infection showed that the time period between the 2nd and the 5th day after spray is critical in the pathogenic process. CONCLUSION: These results found the phenomenon that Ma initiatively broke host hemocytes, revealed the correlation between the virulence of Ma and the changes of enzymes activities in host induced by Ma, and clarified the critical period in the infection of Ma. So, these results should provide guidance for the construction of efficient biocontrol Ma strains.
Asunto(s)
Hemocitos , Hypocreales , Proteínas de Insectos , Locusta migratoria , Oxidorreductasas , Animales , Hemocitos/enzimología , Hemocitos/inmunología , Hemocitos/microbiología , Hypocreales/inmunología , Hypocreales/metabolismo , Hypocreales/patogenicidad , Proteínas de Insectos/inmunología , Proteínas de Insectos/metabolismo , Locusta migratoria/enzimología , Locusta migratoria/inmunología , Locusta migratoria/microbiología , Oxidorreductasas/inmunología , Oxidorreductasas/metabolismo , Control Biológico de VectoresRESUMEN
Insect cell cultures played central roles in unraveling many insect physiological and immunological processes. Regardless, despite imminent needs, insect cell lines were developed primarily from Dipteran and Lepidopteran orders, leaving many important insects such as Orthopteran locusts under-represented. Besides the lack of cell lines, the slow progress in development of in vitro techniques is attributed to poor communications between different laboratories regarding optimized primary cell cultures. Therefore, we report here about methods developed for primary cell culture of Locusta migratoria hemocyte and phagocytic tissue cells by which we could maintain viable hemocytes in vitro for over 5 d and phagocytic tissue cells for over 12 d. 2-Mercaptoethanol and phenyl-thiourea supplements in Grace's medium together with addition of fetal bovine serum 30 min after cell seeding resulted in a successful setup of the primary cell cultures and a week-long survival of the hemocytes and phagocytic tissue cells in vitro.
Asunto(s)
Locusta migratoria/citología , Fagocitosis/inmunología , Cultivo Primario de Células , Animales , Hemocitos/citología , Locusta migratoria/inmunologíaRESUMEN
BACKGROUND: The migratory locust, Locusta migratoria manilensis, is an immensely destructive agricultural pest that forms a devastating and voracious gregarious phase. The fungal insect pathogen, Metarhizium acridum, is a specialized locust pathogen that has been used as a potent mycoinsecticide for locust control. Little, however, is known about locust immune tissue, i.e. fat body and hemocyte, responses to challenge by this fungus. METHODS: RNA-seq (RNA sequencing) technology were applied to comparatively examine the different roles of locust fat body and hemocytes, the two major contributors to the insect immune response, in defense against M. acridum. According to the sequence identity to homologies of other species explored immune response genes, immune related unigenes were screened in all transcriptome wide range from locust and the differential expressed genes were identified in these two tissues, respectively. RESULTS: Analysis of differentially expressed locust genes revealed 4660 and 138 up-regulated, and 1647 and 23 down-regulated transcripts in the fat body and hemocytes, respectively after inoculation with M. acridum spores. GO (Gene Ontology) enrichment analysis showed membrane biogenesis related proteins and effector proteins significantly differentially expressed in hemocytes, while the expression of energy metabolism and development related transcripts were enriched in the fat body after fungal infection. A total of 470 immune related unigenes were identified, including members of the three major insect immune pathways, i.e. Toll, Imd (immune deficiency) and JAK/STAT (janus kinase/signal transduction and activator of transcription). Of these, 58 and three were differentially expressed in the insect fat body or hemocytes after infection, respectively. Of differential expressed transcripts post challenge, 43 were found in both the fat body and hemocytes, including the LmLys4 lysozyme, representing a microbial cell wall targeting enzyme. CONCLUSIONS: These data indicate that locust fat body and hemocytes adopt different strategies in response to M. acridum infection. Fat body gene expression after M. acridum challenge appears to function mainly through activation of innate immune related genes, energy metabolism and development related genes. Hemocyte responses attempt to limit fungal infection primarily through regulation of membrane related genes and activation of cellular immune responses and release of humoral immune factors.
Asunto(s)
Locusta migratoria/inmunología , Locusta migratoria/microbiología , Metarhizium/fisiología , Transcriptoma/inmunología , Animales , Locusta migratoria/genética , Datos de Secuencia Molecular , Análisis de Secuencia de ADN , Transcriptoma/genéticaRESUMEN
The locust cellular defense is mediated by hemocytes and hematopoietic tissue. In Locusta migratoria, the hemocytes and hematopoietic tissue mutually assist each other in clearing invading pathogens from circulation. A ß-1, 3-glucan infection induces nodule formation and apoptotic, TUNEL positive, cells in the hematopoietic tissue and massive loss of hemocytes in the circulation, calling for instant proliferation of hemocytes and hematopoietic tissue cells to assure continued host cellular defense. As the locust hematopoietic tissue persists at the adult stage, it was originally designated as being the major source for the replenishment process. Revisiting post infection hemocyte proliferation, using immunofluorescence based tests for DNA synthesis and mitosis, evidenced the lack of ß-1, 3-glucan induced cell proliferation in the hematopoietic tissue. Instead these tests identified the circulating hemocytes as the major source for hemocyte replenishment in the circulation. The hematopoietic tissue, however, undergoes a continuous, slow and infection independent regeneration, thereby accumulating potential phagocytes despite infection, and might serve a prophylactic role in containing pathogens in this swarming insect.
Asunto(s)
Apoptosis/inmunología , Hemocitos/citología , Hemocitos/inmunología , Inmunidad Celular/inmunología , Locusta migratoria/inmunología , Animales , Candida albicans/inmunología , Proliferación Celular , Sistema Hematopoyético/inmunología , Locusta migratoria/genética , Fagocitos/citología , Fagocitos/inmunología , Fagocitosis/inmunología , Proteoglicanos , Sarcina/inmunología , beta-Glucanos/inmunologíaRESUMEN
Insect immune responses are precisely regulated to maintain immune balance. In this study, the Fas-associated factor 1 (FAF1) gene of Locusta migratoria manilensis, a homologue of the caspar gene that functions as a specific negative regulator in the antibacterial immunity pathway, was cloned. Gene expression analysis showed that FAF1 was expressed throughout the developmental stages and in all tested tissues, but its transcription levels varied significantly. Thus, FAF1 appears to be tightly regulated and is probably involved in multiple physiological processes. In addition, the antimicrobial peptide gene prolixicin was cloned and characterized. After bacterial challenge, prolixicin was rapidly up-regulated, whereas FAF1 was markedly down-regulated. This result was consistent with the observation that prolixicin was hyperactivated when FAF1 was suppressed by RNA interference. Moreover, after bacterial infection, the survival rate of FAF1-knockdown locusts was much higher than that of the wild-type. Taken together, these findings strongly suggest that FAF1 shares a similar function as caspar in Drosophila and may be involved in the negative regulation of antibacterial immunity in locusts.
Asunto(s)
Proteínas de Insectos/metabolismo , Locusta migratoria/inmunología , Proteínas Adaptadoras Transductoras de Señales , Animales , Proteínas Reguladoras de la Apoptosis , Embrión no Mamífero/metabolismo , Escherichia coli , Femenino , Técnicas de Silenciamiento del Gen , Locusta migratoria/metabolismo , Masculino , Ninfa/metabolismo , Interferencia de ARN , Análisis de Secuencia de ADNRESUMEN
Pattern recognition proteins, which form part of the innate immune system, initiate host defense reactions in response to pathogen surface molecules. The pattern recognition protein ß-1,3-glucan recognition protein (ßGRP) binds to ß-1,3-glucan on fungal surfaces to mediate melanization via the prophenoloxidase (PPO)-activating cascade. In this study, cDNA encoding a 53-kDa ßGRP (LmßGRP) was cloned from Locusta migratoria manilensis. LmßGRP mRNA shown to be constitutively expressed specifically in hemocytes and was highly upregulated following fungal infection. LmßGRP-silenced (LmßGRP-RNAi) mutant locusts exhibited significantly reduced survival rate following fungal infection (Metarhizium acridum) compared with the wild-type. Furthermore, LmßGRP-RNAi mutants exhibited abnormally loose stools indicative of a gut defect. 16S rRNA gene analysis detected the opportunistic pathogenic bacterium, Vibrio vulnificus in LmßGRP mutant but not wild-type locusts, suggesting changes in the composition of gut bacterial communities. These results indicate that LmßGRP is essential to gut immunity in L. migratoria manilensis.
Asunto(s)
Proteínas Portadoras/inmunología , Proteínas de Insectos/inmunología , Lectinas/inmunología , Locusta migratoria/inmunología , Locusta migratoria/microbiología , Animales , Bacterias , Proteínas Portadoras/metabolismo , Hongos , Tracto Gastrointestinal/inmunología , Tracto Gastrointestinal/microbiología , Proteínas de Insectos/metabolismo , Lectinas/metabolismo , Filogenia , beta-Glucanos/metabolismoRESUMEN
The insect immune response to fungal infection is poorly understood at the molecular level. To explore the molecular basis of this process, a novel method to analyze the gene transcripts of insects in response to pathogenic fungus was established. A normalized cDNA library based on the SMART method combined with DSN (duplex-specific nuclease) treatment was constructed using mRNA extracted from the fat body and hemocytes of Locusta migratoria manilensis 6-24h after being topically infected with Metarhizium anisopliae var. acridum. Analysis of 259 unigenes out of 303 sequenced inserts from the cDNA library revealed that the cDNA library was not contaminated with M. anisopliae transcripts and validated the presence of the immune-related genes characterized here. These results suggest that this method overcame the difficulties of contamination from a fungal source in constructing the host cDNA library from mycosed insects and proved that this method is reliable and feasible for investigation of host genes in response to fungal infection. Further studies of the expressed sequence tags from this library will provide insights into the molecular basis of insect immune response to fungal infection.
Asunto(s)
Etiquetas de Secuencia Expresada , Biblioteca de Genes , Inmunidad Innata/genética , Locusta migratoria/genética , Locusta migratoria/microbiología , Metarhizium , Animales , Secuencia de Bases , Biología Computacional , Cuerpo Adiposo/metabolismo , Hemocitos/metabolismo , Locusta migratoria/inmunología , Datos de Secuencia Molecular , Oligonucleótidos/genética , ARN Mensajero/metabolismo , Análisis de Secuencia de ADNRESUMEN
Glycoproteins play important roles in insect physiology. Infection with pathogen always results in the differential expression of some glycoproteins, which may be involved in host-pathogen interactions. In this report, differentially-expressed glycoproteins from the hemolymph of locusts infected with Metarhizium anisopliae were analyzed by two-dimensional electrophoresis (2-DE) and PDQuest software. The results showed that 13 spots were differentially expressed, of which nine spots were upregulated and four were downregulated. Using MS/MS with de novo sequencing and NCBI database searches, three upregulated proteins were identified as locust transferrin, apolipoprotein precursor, and hexameric storage protein 3. These proteins have been reported to be involved in the insect innate immune response to microbial challenge. Due to the limited available genome information and protein sequences of locusts, the possible functions of the other 10 differentially-expressed spots remain unknown.
Asunto(s)
Glicoproteínas/biosíntesis , Hemolinfa/química , Proteínas de Insectos/biosíntesis , Locusta migratoria/parasitología , Metarhizium/inmunología , Animales , Electroforesis en Gel de Poliacrilamida , Glicoproteínas/análisis , Glicoproteínas/genética , Interacciones Huésped-Parásitos , Proteínas de Insectos/análisis , Proteínas de Insectos/genética , Locusta migratoria/inmunología , Mapeo PeptídicoRESUMEN
Two isolates of Metarhizium anisopliae var acridum were tested for their effects on the locust immune system and for comparison with the effects of challenge by injection with laminarin. Isolate IMI 330189 (referred to hereafter as Met 189) is highly pathogenic whether applied topically as conidia or injected as blastospores. However, isolate ARSEF 728 (referred to hereafter as Met 728) is pathogenic only when injected as blastospores, suggesting that the lack of pathogenicity of topically applied conidia from this isolate is due to a failure to penetrate the insect cuticle and gain access to the haemocoel. After topical application of conidia from Met 189, no activation of prophenoloxidase is detected, but injection of blastospores from Met 189 brings about a transient increase in phenoloxidase activity in the haemolymph in both adult locusts and 5th instar nymphs, although this does not prevent fungal-induced mortality. Co-injection of adipokinetic hormone-I (AKH-I) with blastospores prolongs the activation of prophenoloxidase in the haemolymph of adult locusts, and enhances it in nymphs. It is argued that the lack of activation of prophenoloxidase in nymphs shown previously (Mullen, L., Goldsworthy, G., 2003. Changes in lipophorins are related to the activation of phenoloxidase in the haemolymph of Locusta migratoria in response to injection of immunogens. Insect Biochemistry and Molecular Biology 33, 661-670), reflects differences in the sensitivity of the immune system between adults and nymphs rather than distinct qualitative differences, and this is confirmed in this study by the demonstration that doses of laminarin higher than those used previously (>or=100 microg) do activate the prophenoloxidase cascade in 5th instar nymphs. Nodules are formed in locusts of all ages in response to fungal infection or injection of laminarin, although there is wide variation in the number, size and distribution of nodules formed. During the examination of 5th instar nymphs for nodule formation, a previously unknown phenomenon was observed in which the salivary glands melanise in response to injections of blastospores or high doses of laminarin. In c. 85% of such nymphs, this reaction is so strong that the whole salivary gland is intensely black. Such a response is not observed in the salivary glands of mature adult locusts.
Asunto(s)
Catecol Oxidasa/metabolismo , Precursores Enzimáticos/metabolismo , Hormonas de Insectos/farmacología , Locusta migratoria/inmunología , Hongos Mitospóricos/fisiología , Administración Tópica , Animales , Femenino , Glucanos , Hemolinfa/metabolismo , Inyecciones , Locusta migratoria/efectos de los fármacos , Locusta migratoria/microbiología , Masculino , Hongos Mitospóricos/patogenicidad , Ninfa/efectos de los fármacos , Ninfa/inmunología , Ninfa/microbiología , Polisacáridos/administración & dosificación , Transducción de Señal/efectos de los fármacos , Esporas Fúngicas/patogenicidad , Esporas Fúngicas/fisiologíaRESUMEN
Photorhabdus is an entomopathogenic bacterium belonging to the Enterobacteriaceae. The genome of the TT01 strain of Photorhabdus luminescens was recently sequenced and a large number of toxin-encoding genes were found. Genomic analysis predicted the presence on the chromosome of genes encoding a type three secretion system (TTSS), the main role of which is the delivery of effector proteins directly into eukaryotic host cells. We report here the functional characterization of the TTSS. The locus identified encodes the secretion/translocation apparatus, gene expression regulators and an effector protein - LopT - homologous to the Yersinia cysteine protease cytotoxin YopT. Heterologous expression in Yersinia demonstrated that LopT was translocated into mammal cells in an active form, as shown by the appearance of a form of the RhoA GTPase with modified electrophoretic mobility. In vitro study showed that recombinant LopT was able to release RhoA and Rac from human and insect cell membrane. In vivo assays of infection of the cutworm Spodoptera littoralis and the locust Locusta migratoria with a TT01 strain carrying a translational fusion of the lopT gene with the gfp reporter gene revealed that the lopT gene was switched on only at sites of cellular defence reactions, such as nodulation, in insects. TTSS-mutant did not induce nodule formation and underwent phagocytosis by insect macrophage cells, suggesting that the LopT effector plays an essential role in preventing phagocytosis and indicating an unexpected link between TTSS expression and the nodule reaction in insects.
Asunto(s)
Proteínas Bacterianas/fisiología , Locusta migratoria/microbiología , Fagocitosis/inmunología , Photorhabdus/metabolismo , Spodoptera/microbiología , Secuencia de Aminoácidos , Animales , Proteínas Bacterianas/genética , Proteínas Bacterianas/inmunología , Membrana Celular/metabolismo , Cisteína Endopeptidasas/genética , GTP Fosfohidrolasas/metabolismo , Genoma Bacteriano , Proteínas Fluorescentes Verdes/genética , Células HeLa , Humanos , Locusta migratoria/inmunología , Locusta migratoria/ultraestructura , Datos de Secuencia Molecular , Photorhabdus/inmunología , Photorhabdus/ultraestructura , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Spodoptera/inmunología , Spodoptera/ultraestructura , Proteína de Unión al GTP rhoA/metabolismoRESUMEN
BACKGROUND: Recent reports of fatal asthma cases associated with swarms of locusts affecting African countries have highlighted the importance of this insect in causing asthma morbidity and mortality. However, only limited information is available about the allergic health outcomes such as asthma and its determinants in exposed individuals. In this study, workers exposed to the African migratory locust Locusta migratoria were evaluated for allergic health outcomes as well as the nature of the offending allergens. METHODS: Ten scientists and technicians exposed to locusts in a laboratory were investigated for locust-related allergy using questionnaires and immunological tests. The presence of allergy was determined by quantification of specific IgE and IgG to L. migratoria using the UniCAP system and via skin-prick testing (SPT). The allergens were characterized by Western blot and ImmunoCAP inhibition assays. RESULTS: Six of the 10 workers experienced symptoms ranging from urticaria and rhinoconjuctivitis to asthma. Seven individuals demonstrated sensitivity on SPT and five had specific IgE antibodies to L. migratoria. Significant cross-reactivity was demonstrated for allergens in the locust faeces, body and wings but not to cockroach allergens. Novel allergens with molecular weights of approximately 70 kDa were identified in locust wings, which are distinctly different from other known allergen sources from locusts. CONCLUSION: Exposure to L. migratoria allergens is a potential sensitizer in exposed individuals. Raised levels of locust-specific IgE can be readily quantified. The wings of this insect species have been identified as a novel allergen source.