Asunto(s)
Herpesvirus Humano 4 , Mononucleosis Infecciosa/complicaciones , Serositis/virología , Membrana Serosa/virología , Enfermedad Aguda , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Niño , Preescolar , Femenino , Humanos , Lactante , Mononucleosis Infecciosa/diagnóstico , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
Peritonitis of viral etiology is rarely reported in the literature; a prior report described a patient undergoing continuous ambulatory peritoneal dialysis who had the disease. We report a case of primary herpetic peritonitis (the agent of which was typed by polymerase chain reaction as herpes simplex virus biotype 1), which caused intestinal perforation, and we review the current literature and provide possible pathophysiologic mechanisms.
Asunto(s)
Herpes Simple/complicaciones , Herpesvirus Humano 1 , Perforación Intestinal/etiología , Peritonitis/virología , Femenino , Herpesvirus Humano 1/aislamiento & purificación , Humanos , Técnicas para Inmunoenzimas , Cuerpos de Inclusión Viral , Persona de Mediana Edad , Peritonitis/complicaciones , Membrana Serosa/virologíaRESUMEN
The prevalence and cellular distribution of human herpesvirus 7 (HHV-7) in archival labial salivary glands was analysed for virus-specific DNA sequences by polymerase chain reaction (PCR) and in situ hybridization signals. In addition, the cellular expression of HHV-7-encoded protein was detected by immunohistochemical staining with a virus-specific monoclonal antibody. Eleven of 20 samples were positive for the HHV-7 DNA sequence by PCR. Eighteen of 20 tissues analysed by in situ hybridization showed signals in ductal, serous and mucous cells. Some nuclei of these cells and also the myoepithelial population were positive. In immunolocalization studies, all 20 salivary glands consistently showed HHV-7-expressed protein in the cytoplasm of ductal cuboidal and columnar cells. The protein was also found in the cytoplasm of mucous and serous acinar cells that were immunopositive for HHV-7. The observations are consistent with the suggestion that the labial salivary gland is a site for virus replication, potential persistence and a source of infective HHV-7 in saliva.
Asunto(s)
Herpesvirus Humano 7/aislamiento & purificación , Glándulas Salivales/virología , Adolescente , Adulto , Anticuerpos Monoclonales , Southern Blotting , Núcleo Celular/ultraestructura , Núcleo Celular/virología , Niño , Citoplasma/ultraestructura , Citoplasma/virología , ADN Viral/análisis , Células Epiteliales/citología , Células Epiteliales/virología , Femenino , Regulación Viral de la Expresión Génica , Infecciones por Herpesviridae , Herpesvirus Humano 7/genética , Humanos , Inmunohistoquímica , Hibridación in Situ , Masculino , Membrana Mucosa/citología , Membrana Mucosa/virología , Reacción en Cadena de la Polimerasa , Saliva/virología , Conductos Salivales/citología , Conductos Salivales/virología , Glándulas Salivales/citología , Análisis de Secuencia de ADN , Membrana Serosa/citología , Membrana Serosa/virología , Proteínas Virales/análisis , Proteínas Virales/genética , Latencia del Virus , Replicación ViralRESUMEN
Reports connect Kaposi's sarcoma-associated herpesvirus (KSHV) with various types of Kaposi's sarcoma (KS) and body-cavity-based lymphomas (primary effusion lymphomas). KSHV DNA sequences have also been detected in a few non-KS malignant vascular tumors. To determine whether KSHV is associated with malignant vascular tumors involving the body cavities, 11 primary vascular tumors of the serous membranes (4 angiosarcomas, 7 hemangioendotheliomas) were tested for KSHV sequences by polymerase chain reaction and Southern blot hybridization. The tumors were from patients who were not known to be immunocompromised. KSHV sequences were detected in the penile KS control but were absent in all 11 specimens studied. Our results were consistent with previous reports that KSHV sequences were rarely detected in non-KS vascular lesions. The results also suggested that although KSHV sequences might be present in primary effusion tumors such as primary effusion lymphoma, they might not be found in other tumors related to body cavities or to serous membranes.