RESUMEN
Chaperone-mediated autophagy (CMA) is the most selective form of lysosomal proteolysis, in which proteins are individually selected for lysosomal degradation. CMA degradation targets bear a pentapeptide consensus motif that is recognized by the cytosolic chaperone HSPA8 (Hsc70), which participates in the trafficking of the target to the lysosomal surface. From there, it is translocated into the lysosomal lumen, independent of vesicle fusion, in a process dependent upon the lysosomal transmembrane protein LAMP2A. There are limited tools for studying CMA in whole cells and tissues, and many of the best techniques for studying CMA rely on the preparation of lysosome enriched fractions. Such experiments include (1) the in vitro evaluation of CMA substrate uptake activity, (2) the characterization of changes to lysosomal resident and CMA regulatory proteins, and (3) lysosomal targetomics, i.e., the use of quantitative proteomics to characterize lysosomal degradation targets. Previous studies using discontinuous metrizamide gradients have shown that a subpopulation of liver lysosomes is responsible for the majority of CMA activity ("CMA+ "). These CMA+ lysosomes are low density and have higher levels of MTORC2 relative to the "CMA- " lysosomes, which are high density and have higher levels of MTORC1. Because of safety concerns surrounding metrizamide, however, this compound is difficult to obtain, and it is impractically expensive. Here, we have provided protocols for isolation of lysosomal subpopulations for CMA-related analyses from mouse liver using Histodenz, a safe and affordable alternative to metrizamide. Supplementary protocols show how to perform CMA activity assays with appropriate statistical analysis, and how to analyze for lysosomal breakage/membrane integrity. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol: Isolation of lysosomal subpopulations from mouse liver using discontinuous Histodenz gradients Alternate Protocol: Isolation of lysosomes from cultured cells using discontinuous Histodenz gradients Support Protocol 1: Verifying enrichment of lysosomal markers in lysosome-enriched fractions Support Protocol 2: Measuring in vitro uptake of CMA substrates Support Protocol 3: Measuring lysosomal membrane integrity by hexosaminidase assay.
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Autofagia Mediada por Chaperones , Animales , Ratones , Metrizamida , Lisosomas , beta-N-Acetilhexosaminidasas , BioensayoRESUMEN
OBJECTIVES: Management of the neck in patients with oral squamous cell carcinoma (OSCC) is pivotal to oncologic control and survival. However, there is controversy regarding necessity of neck dissection (ND) in patients with clinically node-negative neck. We aimed to assess risk factors for occult metastasis and to explore whether the presence of occult lymph node metastases (LNMs) has an impact on recurrence and survival. MATERIAL AND METHODS: A retrospective cohort study was performed including patients with primary OSCC who underwent radical tumor resection and ND in a high-volume center adhering to the prevailing German guideline. The ND was performed according to a standardized approach. RESULTS: Four hundred twenty-one patients with primary surgically treated OSCC were included. The incidence of occult metastasis was 14.49%. A pathological T stage > 1 (multivariate analysis, odds ratio (OR) 3.958, p = 0.042) and the presence of extranodal extension in LNMs (multivariate analysis, OR 0.287, p = 0.020) were identified as independent risk factors for occult metastasis. When comparing patients with and without occult metastasis, there were no significant differences in terms of progression-free survival (log-rank, p = 0.297) and overall survival (log-rank, p = 0.320). There were no cases of ipsilateral neck recurrence. One patient developed contralateral neck metastasis; however, he initially presented with a unilateral pT1 pN0 tumor. CONCLUSIONS: Overall, our findings suggest that conducting a standardized approach in ND should be applied in terms of management of the neck in order to maintain survival rates and to prevent neck recurrence in OSCC patients. CLINICAL RELEVANCE: None of the risk factors for occult metastasis can be reliably assessed preoperatively. Although elective ND does not guarantee the complete prevention of neck recurrence, it increases the likelihood of either timely removal of micrometastases or strengthens the justification for adjuvant therapy. Consequently, this approach leads to improvements in clinical outcomes.
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Carcinoma de Células Escamosas , Neoplasias de Cabeza y Cuello , Metrizamida/análogos & derivados , Neoplasias de la Boca , Masculino , Humanos , Carcinoma de Células Escamosas/cirugía , Carcinoma de Células Escamosas de Cabeza y Cuello , Disección del Cuello , Neoplasias de la Boca/cirugía , Estudios Retrospectivos , Metástasis LinfáticaAsunto(s)
Angiografía/historia , Medios de Contraste/historia , Arteria Femoral , Animales , Circulación Sanguínea/efectos de los fármacos , Medios de Contraste/toxicidad , Diatrizoato de Meglumina/toxicidad , Perros , Historia del Siglo XX , Yotalamato de Meglumina/toxicidad , Metrizamida/toxicidad , Flujo Sanguíneo Regional/efectos de los fármacos , Ácidos Triyodobenzoicos/toxicidadRESUMEN
Eosinophils are multifunctional leukocytes classically described as being involved in helminth parasitic infections and allergic diseases. Previously restricted to an exclusive role in the release of cytotoxic mediators, they are now also considered to be immunoregulatory cells and potential effectors in innate immune responses. Eosinophils are mainly found in tissues, so specific procedures are needed for their isolation from venous blood and for functional assays. Murine models are very useful for the dissection of eosinophil physiology in vivo. But murine eosinophils significantly differ from human ones. A complete understanding of eosinophil biology therefore requires comparative study of eosinophils from different mammalian species. We summarize here the main experimental protocols used to study human, mouse, and rat eosinophil biology. We focus on technical improvements of existing methods that optimize purification and in vitro functional studies of eosinophils.
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Técnicas de Cultivo de Célula/métodos , Eosinófilos/inmunología , Eosinófilos/parasitología , Inmunidad Innata/inmunología , Neoplasias/inmunología , Animales , Biomarcadores/metabolismo , Separación Celular , Células Cultivadas , Citotoxicidad Inmunológica , Eosinófilos/citología , Citometría de Flujo , Humanos , Metrizamida , Ratones , Ratas , Receptores Inmunológicos/metabolismo , SolucionesRESUMEN
Standing myelography in the horse has been previously described. In that study, metrizamide was used and significant complications were reported. In recent years, the introduction of less-toxic nonionic contrast media has reduced the incidence of complications. This study was undertaken to determine whether standing myelography using a nonionic contrast medium could provide a diagnostic study and be performed safely in the equine patient. Standing myelography was performed in eight horses. The contrast medium used was iohexol. In five horses a myelogram of diagnostic quality was achieved; in one horse contrast flowed only to the level of C6 and in two horses contrast medium did not reach the cervical subarachnoid space. Owing to the difficulty in achieving good flow of the contrast medium in some horses, this procedure may be of limited utility. However, if puncture of the lumbosacral subarachnoid space can be achieved easily and quickly, standing myelography may be a clinically useful procedure. It may be attempted in cases in which the economic value of the patient makes myelography under general anesthesia impractical. In patients presenting for evaluation of ataxia it may be possible to perform a standing myelogram at the time of CSF sample collection from the lumbosacral space.
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Medios de Contraste/administración & dosificación , Enfermedades de los Caballos/diagnóstico por imagen , Metrizamida/administración & dosificación , Mielografía/veterinaria , Médula Espinal/diagnóstico por imagen , Enfermedades de la Columna Vertebral/veterinaria , Animales , Vértebras Cervicales/diagnóstico por imagen , Femenino , Caballos , Inyecciones Espinales/veterinaria , Masculino , Postura , Enfermedades de la Columna Vertebral/diagnóstico por imagen , Espacio Subaracnoideo/diagnóstico por imagenRESUMEN
Hepatic stellate cells (HSCs) are routinely prepared by collagenase/pronase digestion of liver using a perfusion system and subsequent fractionation of the heterogeneous cell suspension on continuous density gradients made out of Nycodenz, metrizamide, stractan, or percoll. Because of their lipid content, stellate cells are the least dense fraction of the nonparenchymal cells, and during centrifugation they float effectively away from other hepatic cells resulting in preparations containing almost 80% stellate cells. The degree of purity can be increased by further enrichment of cells by methods like centrifugal elutriation or Scatter-activated cell sorting. We present a detailed protocol from our laboratory to obtain a high number of pure, viable, freshly isolated hepatic stellate cells from rat liver. This two-step protocol (collagenase/pronase digestion and Nycodenz gradient) yields a preparation of approx 4-5 x 107 cells enriched in 74% HSC having a viability of at least 76% as estimated by Trypan blue exclusion test. Further purification by centrifugal elutriation results in virtually pure HSC preparations ( >98%).
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Técnicas de Cultivo de Célula/métodos , Hepatocitos/citología , Hígado/citología , Animales , Diferenciación Celular , Separación Celular , Supervivencia Celular , Centrifugación por Gradiente de Densidad , Colagenasas/metabolismo , Medios de Cultivo , Fibroblastos/metabolismo , Yohexol/farmacología , Metabolismo de los Lípidos , Hígado/metabolismo , Hígado/patología , Masculino , Metrizamida/farmacología , Modelos Químicos , Perfusión , Ratas , Ratas Sprague-Dawley , Azul de Tripano/farmacologíaRESUMEN
Specific targeting of liposome-formulated cytotoxic drugs or antigens to receptors expressed selectively on target cells represents an effective strategy for increasing the pharmacological efficacy of the delivered molecules. We have developed a feasible technique to selectively attach antibodies and fragments thereof, but also small-mol-wt ligands such as peptides, carbohydrates, or any molecules that recognize and bind target antigens or receptors to the surface of small unilamellar liposomes. Our concept is based on the site-specific functionalization of the ligands to be attached to the liposomes by thiol groups. These thiol groups can easily be introduced to antibodies or peptides by addition of cysteines, preferably at sites that do not interfere with the receptor binding domains. Optimally, the site-specific modification is introduced at the C-terminal end of the ligand, separated by an inert spacer sequence located between the thiols and the specific part of the ligand. The thiol-reactive molecules on the liposome surface are maleimides that are linked to phospholipids composing the liposome bilayer membrane. We illustrate the coupling method of a functionalized single-chain antibody fragment with binding specificity to ED-B fibronectin, an isoform of fibronectin exclusively expressed in tumor tissues, to long circulating small unilamellar poly(ethylene glycol) liposomes.
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Fragmentos de Inmunoglobulinas/inmunología , Fragmentos de Inmunoglobulinas/toxicidad , Liposomas , Neoplasias/tratamiento farmacológico , Neoplasias/inmunología , Línea Celular Tumoral , Sistemas de Liberación de Medicamentos , Humanos , Fragmentos de Inmunoglobulinas/química , Lípidos/química , Liposomas/síntesis química , Liposomas/química , Metrizamida , Estructura MolecularRESUMEN
BACKGROUND: Treatment with anti-CD154 monoclonal antibody (mAb) plus a donor-specific transfusion (DST) of spleen cells prolongs skin allograft survival in mice through a mechanism involving deletion of host alloreactive CD8(+) T cells. It is unknown if other lymphohematopoietic cell populations can be used as a DST. METHODS: Murine recipients of allogeneic skin grafts on day 0 were either untreated or given a DST on day -7 plus 4 doses of anti-CD154 mAb on days -7, -4, 0, and +4. Deletion of CD8(+) alloreactive cells was measured using "synchimeric" CBA recipients, which circulate trace populations of TCR transgenic alloreactive CD8(+) T cells. RESULTS: Transfusion of splenocytes, thymocytes, lymph node cells, or buffy coat cells led to prolonged skin allograft survival in recipients treated with anti-CD154 mAb. In contrast, bone marrow DST failed to delete host alloreactive CD8(+) T cells and was associated with brief skin allograft survival. Transfusions consisting of bone marrow-derived dendritic cells or a mixture of splenocytes and bone marrow cells were also ineffective. CONCLUSIONS: Donor-specific transfusions of splenocytes, thymocytes, lymph node cells, or buffy coat cells can prolong skin allograft survival in recipients treated with costimulation blockade. Bone marrow cells fail to serve this function, in part by failing to delete host alloreactive CD8(+) T cells, and they may actively interfere with the function of a spleen cell DST. The data suggest that transplantation tolerance induction protocols that incorporate bone marrow cells to serve as a DST may not be effective.
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Anticuerpos Monoclonales/uso terapéutico , Transfusión Sanguínea , Células de la Médula Ósea/fisiología , Ligando de CD40/fisiología , Supervivencia de Injerto , Trasplante de Piel , Animales , Linfocitos T CD8-positivos/inmunología , Células Dendríticas/trasplante , Depleción Linfocítica , Metrizamida/farmacología , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Bazo/citología , Trasplante HomólogoRESUMEN
El objetivo del presente trabajo es exponer un grupo de compuestos iodados con tres y seis átomos de iodo en su molécula que son derivados del ácido benzoico y son utilizados en Radiología como medios de contraste de gran interés clínico. Se estudian sus constantes físico-químicas y las ventajas que presentan los contrastes no iónicos sobre los iónicos. Se describen también los posibles efectos colaterales indeseables en un pequeño pero importante número de pacientes que pueden producirse por su inyección intravascular (AU)
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Humanos , Medios de Contraste/efectos adversos , Compuestos de Yodo/efectos adversos , Yohexol/farmacocinética , Metrizamida/farmacocinética , Yotalamato de Meglumina/farmacocinética , Diatrizoato de Meglumina/toxicidad , Espectroscopía de Resonancia Magnética/métodosRESUMEN
Knowledge of the blast phenotype in myelodysplastic syndrome (MDS) would be valuable, as in other malignancies, but remains sparse. This is mainly because MDS blasts are a minor population in clinical samples, making analysis difficult. Thus, for this blast phenotype study, we prepared blast-rich specimens (using a new density centrifugation reagent for harvesting blasts) from blood and marrow samples of 95 patients with various MDS subtypes and 21 patients with acute leukemia transformed from MDS (AL-MDS). Flow cytometry revealed that a high proportion of the enriched blast cells (EBCs) from almost all patients showed an immunophenotype of committed myeloid precursors (CD34(+)CD38(+)HLA-DR(+)CD13(+)CD33(+)), regardless of the disease subtype. The cytochemical reaction for myeloperoxidase was negative in 58% of the cases. Thus, the EBC phenotype is more immature in MDS than in de novo acute myeloid leukemia. MDS EBCs often coexpressed stem cell antigens and late-stage myeloid antigens asynchronously, but rarely expressed T- and B-lymphoid cell-specific antigens. Markers for myeloid cell maturation (CD10 and CD15) were more prevalent on EBCs from low-risk MDS (refractory anemia [RA] and RA with ringed sideroblasts), whereas markers for myeloid cell immaturity (CD7 and CD117) were more prevalent on EBCs from high-risk MDS (chronic myelomonocytic leukemia, RA with excess blasts [RAEB], and RAEB in transformation) and AL-MDS. A shift to a more immature phenotype of EBCs, accompanying disease progression, was also documented by sequential phenotyping of the same patients. Further, CD7 positivity of EBCs was an independent variable for a poor prognosis in MDS. These data represent new, valuable information regarding MDS.
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Síndromes Mielodisplásicos/patología , Enfermedad Aguda , Anciano , Antígenos CD/análisis , Estudios de Casos y Controles , Separación Celular/métodos , Centrifugación por Gradiente de Densidad/métodos , Progresión de la Enfermedad , Femenino , Citometría de Flujo , Humanos , Inmunofenotipificación , Leucemia/genética , Leucemia/patología , Masculino , Metrizamida , Persona de Mediana Edad , Síndromes Mielodisplásicos/genética , Fenotipo , Pronóstico , Análisis de SupervivenciaRESUMEN
Myeloblast-rich samples, required for investigation of myeloid malignancies, can be obtained only during the untreated stage of leukemia. Existing methods for myeloblast enrichment have various prerequisites that limit their application. In this new method, a mixture of peripheral blood (Mixed PB) from an acute myeloid leukemia (AML) patient and from a healthy control containing 5% myeloblasts was subjected to density gradient centrifugation using a 14.5% metrizamide solution. Both high purity (86.3% +/- 1.5%) and high recovery of viable myeloblasts were achieved. Close to 100-fold blast enrichment, even from Mixed PB containing only 0.15% myeloblasts, was achieved. Similarly, this method highly enriched myeloblasts from unprocessed samples, including marrow cells, from patients with AML, myelodysplastic syndromes, and chronic myeloid leukemia (purity: 2.7% +/- 2.0% before separation, 56.6% +/- 28.3% after separation) (n = 22). The enriched blasts were suitable for various analyses, eg, flow cytometry, immunocytochemistry, cytochemistry, fluorescence in situ hybridization, and gene analysis.
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Centrifugación por Gradiente de Densidad/métodos , Síndromes Mielodisplásicos/patología , Células Mieloides/patología , Enfermedad Aguda , Células de la Médula Ósea/patología , Separación Celular , Centrifugación por Gradiente de Densidad/normas , Citometría de Flujo , Histocitoquímica , Humanos , Leucemia Mielógena Crónica BCR-ABL Positiva/patología , Leucemia Mieloide/patología , MetrizamidaAsunto(s)
Medios de Contraste/historia , Metrizamida/historia , Médula Espinal/diagnóstico por imagen , Tomografía Computarizada por Rayos X/historia , Medios de Contraste/administración & dosificación , Historia del Siglo XX , Humanos , Inyecciones Espinales/historia , Región Lumbosacra , Metrizamida/administración & dosificación , Neurología/historiaRESUMEN
Linear mitochondrial genomes exist in several yeast species which are closely related to yeast that harbor circular mitochondrial genomes. Several lines of evidence suggest that the conversion from one form to another occurred accidentally through a relatively simple mechanism. Previously, we (L.T. & J.N.) reported the identification of the first mitochondrial telomere-binding protein (mtTBP) that specifically binds a sequence derived from the extreme end of Candida parapsilosis linear mtDNA, and sequence analysis of the corresponding nuclear gene MTP1 revealed that mtTBP shares homology with several bacterial and mitochondrial single-stranded (ss) DNA-binding (SSB) proteins. In this study, the DNA-binding properties of mtTBP in vitro and in vivo were analyzed by electron microscopy (EM). When M13 ssDNA was used as a substrate, mtTBP exhibited similar DNA binding characteristics as human mitochondrial SSB: mtTBP formed protein globules along the DNA substrate, and the bound proteins were randomly distributed, indicating that the binding of mtTBP to M13 ssDNA is not highly cooperative. EM analysis demonstrated that mtTBP is able to recognize the 5' single-stranded telomeric overhangs in their natural context. Using isopycnic centrifugation of mitochondrial lysates of C. papsilosis we show that mtTBP is a structural part of mitochondrial nucleoids of C. parapsilosis and is predominantly bound to the mitochondrial telomeres. These data support a dual role of mtTBP in mitochondria of C. parapsilosis, serving both as a typical mitochondrial SSB and as a specific component of the mitochondrial telomeric chromatin.
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Candida/química , Proteínas de Unión al ADN/metabolismo , Proteínas de Unión al ADN/ultraestructura , Proteínas Fúngicas/metabolismo , Proteínas Fúngicas/ultraestructura , Bacteriófago M13/genética , Western Blotting , Candida/citología , Candida/genética , Centrifugación por Gradiente de Densidad , Cromatina/genética , Cromatina/metabolismo , ADN de Hongos/genética , ADN de Hongos/metabolismo , ADN de Hongos/ultraestructura , ADN Mitocondrial/genética , ADN Mitocondrial/metabolismo , ADN Mitocondrial/ultraestructura , ADN de Cadena Simple/genética , ADN de Cadena Simple/metabolismo , ADN de Cadena Simple/ultraestructura , Proteínas de Unión al ADN/química , Proteínas de Unión al ADN/aislamiento & purificación , Proteínas Fúngicas/química , Proteínas Fúngicas/aislamiento & purificación , Humanos , Metrizamida , Microscopía Electrónica , Unión Proteica , Estructura Cuaternaria de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/ultraestructura , Relación Estructura-Actividad , Especificidad por Sustrato , Telómero/genética , Telómero/metabolismoRESUMEN
Employing antisera against various subfractions of rat liver mitochondria (mitoplast, inner membrane, intermembrane, and matrix) as well as metabolically radiolabeled BRL-3A rat liver cells, we undertook a search for the presence of glycoproteins in this major cellular compartment for which little information in regard to glycoconjugates was available. Subsequent to [35S]methionine labeling of BRL-3A cells, a peptide:N-glycosidase-sensitive protein (45 kDa) was observed by SDS-polyacrylamide gel electrophoresis of the inner membrane immunoprecipitate, which was reduced to a molecular mass of 42 kDa by this enzyme. The 45-kDa protein was readily labeled with [2-3H]mannose, and indeed the radioactivity of the inner membrane immunoprecipitate was almost exclusively present in this component. Moreover, antisera directed against mitochondrial NADH-ubiquinone oxidoreductase (complex I) or F1F0-ATPase (complex V) also precipitated a 45-kDa protein from BRL-3A cell lysates as the predominant mannose-radiolabeled constituent. Endo-beta-N-acetylglucosaminidase completely removed the radiolabel from this glycoprotein, and the released oligosaccharides were of the partially trimmed polymannose type (Glc1Man9GlcNAc to Man8GlcNAc). Cycloheximide as well as tunicamycin resulted in total inhibition of radiolabeling of the inner membrane glycoprotein, and moreover, pulse-chase studies employing metrizamide density gradient centrifugation demonstrated that the glycoprotein was initially present in the endoplasmic reticulum (ER) and subsequently appeared in a mitochondrial location. Early movement of the glycoprotein to the mitochondria after synthesis in the ER was also evident from the limited processing undergone by its N-linked oligosaccharides; this stood in contrast to lysosomal glycoproteins in which we noted extensive conversion to complex oligosaccharides. Our findings suggest that the 45-kDa glycoprotein migrates from ER to mitochondria by the previously observed contact sites between the two organelles. Furthermore, the presence of this glycoprotein in at least two major mitochondrial multienzyme complexes would be consistent with a role in mitochondrial translocations.
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Retículo Endoplásmico/fisiología , Glicoproteínas de Membrana/química , Mitocondrias Hepáticas/química , Amidohidrolasas/metabolismo , Animales , Línea Celular , Centrifugación por Gradiente de Densidad , Complejo I de Transporte de Electrón , Glicosilación , Masculino , Manosa/metabolismo , Metrizamida/metabolismo , NADH NADPH Oxidorreductasas/análisis , Oligosacáridos/análisis , Péptido-N4-(N-acetil-beta-glucosaminil) Asparagina Amidasa , Ratas , Ratas EndogámicasRESUMEN
A method for the isolation of highly purified peroxisomes from guinea pig small intestine was developed. This two-stage process involved a rate-dependent banding of a light-mitochondria lambda-fraction followed by a density-dependent banding of the catalase enriched fractions obtained from the first step, using a horizontal rotor. Furthermore, the subcellular localization of glucose-6-phosphate dehydrogenase (NADP+-dependent) activity in guinea pig small intestine was examined. Analysis of density-gradient fractions indicated that approximately 3-4% of the cellular NADP+-dependent glucose-6-phosphate dehydrogenase activity is associated with peroxisomal fractions and that it is localized to the matrix of peroxisomes. It is therefore suggested that a peroxisomal source of NADPH may be utilized by enzyme systems that use NADPH specifically as a reductant.
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Glucosafosfato Deshidrogenasa/metabolismo , Intestino Delgado/metabolismo , Microcuerpos/enzimología , Animales , Biomarcadores/química , Fraccionamiento Celular/métodos , Centrifugación por Gradiente de Densidad , Cobayas , Masculino , Metrizamida/metabolismo , Microcuerpos/ultraestructura , Microscopía Electrónica , NADP/metabolismo , Octoxinol/farmacologíaRESUMEN
A prospective clinical trial comparing adverse postmyelographic effects and myelographic quality of metrizamide and iohexol was conducted. Using a predetermined, randomized assignment, 24 horses exhibiting neurologic signs were administered either metrizamide (180 mgl/ml) or iohexol (180 mgl/ml) via cerebellomedullary puncture. Each horse was evaluated postmyelographically for adverse effects. Myelographic quality was assessed by a numerical scoring method. Adverse effects were observed more frequently with metrizamide (21) compared with iohexol (6) myelography (p < 0.05). Seizures, intensification of preexisting neurologic signs and prolonged anesthetic recovery were the most common complications after myelography. There was no difference in myelographic quality (p > 0.05). We conclude that iohexol is safer than metrizamide for equine myelography and that quality myelograms can be obtained with either contrast medium.
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Medios de Contraste , Enfermedades de los Caballos/diagnóstico por imagen , Caballos , Yohexol , Metrizamida , Mielografía/veterinaria , Periodo de Recuperación de la Anestesia , Anestesia por Inhalación/veterinaria , Anestesia Intravenosa/veterinaria , Animales , Medios de Contraste/administración & dosificación , Medios de Contraste/efectos adversos , Método Doble Ciego , Femenino , Fiebre/inducido químicamente , Fiebre/veterinaria , Enfermedades de los Caballos/inducido químicamente , Caballos/clasificación , Yohexol/administración & dosificación , Yohexol/efectos adversos , Masculino , Metrizamida/administración & dosificación , Metrizamida/efectos adversos , Mielografía/métodos , Estudios Prospectivos , Punciones/veterinaria , Intensificación de Imagen Radiográfica , Distribución Aleatoria , Seguridad , Convulsiones/inducido químicamente , Convulsiones/veterinaria , Enfermedades de la Médula Espinal/diagnóstico por imagen , Enfermedades de la Médula Espinal/veterinariaRESUMEN
BACKGROUND AND OBJECTIVES: Otogenic cerebrospinal fluid (CSF)fistula referrs to the abnormal communication between the CSF and the middle ear space due to defects in the inner ear, and is one of the causes of recurrent meningitis. MATERIALS AND METHODS: We report on five children with congenital otogenic CSF fistula, presented by recurrent meningitis and confirmed by surgical exploration. We also propose diagnostic steps for detecting otogenic fistula in the children based on our experiences and paper review. RESULTS: We used the metrizamide CT in the diagnostic procedure for most cases, although not all. The temporal bone CT was an useful initial diagnostic step for clinically suspicious cases. CONCLUSION: No one test or combination of specific tests were found to accurately predict the presence or absence of CSF fistula. It is thought that the only way to diagnose the CSF fistula is by surgical exploration. If the CSF fistula was suspected, aggressive diagnostic evaluation was needed in order to prevent recurrence.
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Niño , Humanos , Líquido Cefalorraquídeo , Diagnóstico , Oído Interno , Oído Medio , Fístula , Meningitis , Metrizamida , Recurrencia , Hueso TemporalRESUMEN
An arteriovenous malformation model with a transparent nidus was constructed to investigate the embolization behavior of polyvinyl acetate (PVAc) solution relative to flow velocity in the feeding artery and injection speed. It was found that the liquid embolic material flowed distally when injected too fast or when the flow velocity was too low. In addition, we found that solution consisting of PVAc plus metrizamide was a better embolic material than solution containing only PVAc.